Publications HAL du labo/EPI 37751;1002097

2025

Conference papers

titre
Multi-environment GWAS to decipher the response to natural infections in apricot and peach
auteur
Marie Serrie, Vincent Segura, Alain Blanc, Laurent Brun, Naïma Dlalah, Frédéric Gilles, Laure Heurtevin, Mathilde Le Pans, Véronique Signoret, Sabrina Viret, Jean-Marc Audergon, Bénédicte Quilot, Morgane Roth
article
12th Rosaceae Genomics Conference (RGC 12), May 2025, Sant Feliu de Guíxols, Spain
resume
A great majority of modern apricot and peach varieties remain sensitive to the most problematic pests and diseases. To improve the capacity of varieties to deal with decreased levels of pesticide use, we need a better understanding of resistance and tolerance mechanisms in these crops. To serve this objective, we exposed large and diversified sets of apricot and peach accessions (so-called ‘core-collections’) to natural infections within a multi-environment trial. These core-collections consist in 150 apricot and 206 peach accessions, for which respectively 207,000 and 13,680 SNP data are available, and were observed from 2020 to 2023 at two to three environmentally contrasted locations in South-East of France. Respectively two and five pests and diseases were monitored in apricot and peach. After showing the impact of genetic and GxE effects on phenotypic variance via variance decompositions, we applied several GWAS models to identify associations (i) with conserved effects across environments, (ii), with interactive effects across environments, (iii) with environment-specific effects. We also explored non-additive mechanisms, yielding additional associations. This work led to the identification of 60 high confidence QTLs and 87 candidate genes involving both host-specific and basal resistance mechanisms. Interestingly, candidate regions were enriched for leucine-rich repeat containing receptors (LRR-CRs). We discuss the relevance of environment-dependant QTLs according to the different disease typologies found in this study. This work contributes to the establishment of marker assisted selection strategies to allow for a faster screening of the disease resilience capacity in peach and apricot breeding material.
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A DELETION IN A PHOTOSYNTHESIS-RELATED GENE (PpPSAK) DETERMINES THE ANTHOCYANIN ACCUMULATION IN THE MESOCARP OF FRENCH BLOOD-FLESH PEACHES
auteur
Laure Heurtevin, Carole Confolent, Patrick Lambert, Sylvie Bureau, Thierry Pascal, Zhijun Shen, Bénédicte Quilot-Turion
article
12th Rosaceae Genomics Conference, IRTA, May 2025, Sant Feliu de Guíxols, Spain
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Little is known about the mechanisms by which light controls anthocyanin biosynthesis in fruit flesh. Yet the accumulation of anthocyanin pigments is a major determinant of peach (Prunus persica L. Batch) fruit quality. We studied the recessive blood-flesh trait (bf), identified in French cultivars by mapping and then localized on chromosome 4. On genetic, molecular and transcriptional grounds, we identified the underlying gene linked to this bf trait. A metabolomic study showed that blood-flesh cultivars had high levels of cyanidin-3-glucoside and cyanidin-3-rutinoside in the fruit mesocarp around 45 to 60 days after anthesis. Fine mapping coupled with a positional cloning strategy mapped the bf locus to an 80 kb interval on peach linkage group (LG) 4. Sequence polymorphism analysis of the genes present in this interval identified a single candidate gene for bf as a 21-bp deletion in exon 3 of a PSAK-type gene. A specific marker for the deletion was developed and validated in various genetic backgrounds. A comparative transcriptomic analysis of fruit flesh from a bf and non-bf cultivar at 4 stages of fruit development, in kinetics ranging from 60 days after flowering to fruit ripening, revealed co-expressed genes. Overexpression of PpPSAK in the flesh and midrib of bf fruit correlated with the expression of structural anthocyanin biosynthesis genes. Protein-protein interactions were observed and functional pathways were discussed. This work presents evidence leading to the first discovery of a photosystem-related gene involved in regulating fruit flesh coloration.
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An inter-comparison exercise between spectrometers and handheld sensors to assess biochemical traits of orchard tree leaves
auteur
Karine Adeline, Nathan Sikora, Jean-Baptiste Féret, Sylvain Jay, Morgane Roth, Marie-Noëlle Corre, Sylvie Serino, Valérie Serra, G. Vercambre, Quentin Barra, Nicolas Marlet
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10ème colloque scientifique de la Société Française de Photogrammétrie et Télédétection – Groupe Hyperspectral, May 2025, Grasse, France
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Comment créer des variétés moins gourmandes en pesticides pour l’arboriculture? Exemple des recherches sur l’abricotier et le pêcher à INRAE
auteur
Morgane Roth
article
Conférence pour les clubs Rotary de Sainte Ménéhould et Verdun, Rotary Sainte Menehould, Mar 2025, Valmy, France
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titre
eIF4E1 precision editing provides a solution to the trade-off between the plant development and potyvirus resistance albeit with a low durability
auteur
Jean-Luc Gallois, Kyoka Kuroiwa, Anaë Laurens, Caroline Lebaron, Marianne Mazier
article
20es rencontres de Virologie Végétale, Jan 2025, Aussois, France
resume
components of the plant cell machinery. For example, in Tomato, resistance to the PVY N605 isolate can be achieved by knocking out both eIF4E1 and eIF4E2 genes, but is associated with profound effect on the plant development (Gauffier et al. Plant J., 2016). A solution is to design functional alleles encoding host proteins which retain their initial role in the plant physiology but that the virus cannot recruit any longer. Recently, we showed as a proof of concept the conception of an eIF4E1 resistance allele in cherry tomato obtained by genome editing based on the knowledge resulting from the study of natural functional alleles in crops (Kuroiwa et al., PBJ, 2024). Here, we further assess this allele for its effect on plant development and resistance durability. We launched a whole-plant phenotyping on a set of tomato genotypes where the endogenous eIF4E1gene is edited in the two regions acknowledged to be involved in resistance to potyviruses. We show that the eIF4E1- editing in tomatoes do not affect the plant growth and development in a greenhouse setting. We proceeded with the evaluation of the virus resistance durability. We show that most resistant edited SleIF4E1 suppresses the PVY N605 viral accumulation to a significantly lower level than the wild-type SleIF4E1 but that this resistance can be broken down substantially whithin nine weeks after inoculation. This is confirmed by the virus obtaining signature mutations of resistance breaking. Altogether, this work assesses the efficacy of resistance breeding strategy by the base-editing in tomato SleIF4E1 in an applicational point of view, but calls for further improvement in the resistance allele design and obtention.
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Relations entre virulence, charge virale et transmission du potato virus Y (genre Potyvirus ; famille Potyviridae)
auteur
Lola Chateau, Lucie Tamisier, Thomas Oudin, Roxanne Barosi, Marion Szadkowski, Gregory Girardot, Benoît Moury
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20. Rencontres de virologie végétale (RVV 2025), Jan 2025, Aussois, France
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https://hal.inrae.fr/hal-05147331/file/2025_Chateau_RVV2025.pdf BibTex
titre
Uncovering the genetic basis of quantitative resistance to cucumber mosaic virus in Capsicum annuum using genome-wide association coupled with high-throughput image analysis
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Judith Hirsch, Lucie Tamisier, Marion Szadkowski, Sylvain Piry, Louis McLeod, Anne Massire, Christophe Gros, Benoît Moury, Véronique Lefebvre
article
20es Rencontres de Virologie Végétale (RVV 2025), UMR PHIM, Jan 2025, Aussois, France. pp.C-41
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https://hal.inrae.fr/hal-05147567/file/2025_Hirsch_RVV2025.pdf BibTex

Other publications

titre
Evaluation de la sensibilité variétale aux maladies de la Core Collection abricotier INRAE Gotheron
auteur
Mathilde Le Pans, Pedro Asensio, Jean-Marc Audergon, Laurent Brun, Freddy Combe, Guy Clauzel, Julien Doux, Amandine Fleury, Marie Gaslain, David Gerin-Jean, Luana Gillet, Claire Marrec, Franck Merlin, Léo Moncorger, Frédéric Oboussier, Agnès Pelloie, Océane Perez, Julie Robin, Guillaume Roch, Morgane Roth, Marie Serrie, Véronique Signoret, David Tricon, Sabrina Viret
article
2025
resume
La Core collection abricotiers INRAE est constituée de 150 variétés représentatives de la diversité cultivée mondiale. Un exemplaire de cette Core collection a été implanté sur le site d’INRAE Gotheron en 2018, et a été évalué pour la sensibilité variétale aux maladies en l’absence de protection fongicide de 2020 à 2024. Les approches méthodologiques mises en œuvre et le classement des sensibilités variétales aux maladies seront présentés.
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Poster communications

titre
Deciphering the genetic architecture of plant-pathogen interactions using co-GWAS
auteur
Moustapha Boubacar-Abdou, Vincent Rittener, Pascale Mistral, Karine Leyre, Jacques Lagnel, Patricia Faivre Rampant, Damien Hinsinger, Louis McLeod, Cecile Desbiez, Nathalie Boissot, Lucie Tamisier
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Journées Jeunes Chercheurs du département BAP 2025, May 2025, Bordeaux, France
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The use of resistant varieties is a key strategy for crop protection against pathogens, but its effectiveness is often compromised by the rapid evolution of pathogens. Leveraging the genetic heterogeneity of deployed resistant varieties, through varietal mixtures or crop rotations, emerges as a promising approach to limit pathogen adaptation by constraining their evolution. However, implementing such strategies requires a deep understanding of the genetic architecture of plant-pathogen interactions. This PhD project proposes an innovative methodological framework aimed at characterizing the genetic architecture of interactions between melon (Cucumis melo) and Watermelon Mosaic Virus (WMV). Our objective is to identify the plant QTLs (Quantitative Trait Loci) involved in resistance, as well as the pathogen QTLs controlling virulence. To achieve this, we plan to implement an integrative approach combining: - An experimental co-GWAS approach, by systematically testing all pairwise combinations between 180 melon accessions and 21 sequenced WMV strains under controlled conditions. This approach will enable the detection of associations between genetic variations in the plant and virus, and phenotypes (resistance, virulence). - A natural co-GWAS approach, based on the analysis of the same melon collection naturally infected in the field by aphid vectors of the virus. The viral populations infecting each plant will be sequenced, and association tests will be conducted for all plant-pathogen genetic variant pairs. These analyses will help uncover Gplant × Gpathogen interactions. The originality of this approach lies in the joint analysis of host and pathogen genomes, enabling a detailed understanding of the mechanisms underlying G × G interactions. The results of this PhD work will provide an essential knowledge base for parameterizing models that compare various deployment strategies by estimating their durability.
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https://hal.inrae.fr/hal-05127001/file/Poster_INRAE_Boubacar.pdf BibTex
titre
GWAS On Multiple Traits And Resilience Indicators : A Step Towards Peach Ideotypes Adapted To Pesticide Reduction
auteur
Marie Serrie, Vincent Segura, Jean-Marc Audergon, Morgane Roth, Bénédicte Quilot
article
Rosaceae Genomics Conference 12, May 2025, Sant Feliu de Guíxols, Spain, May 2025, Sant Feliu de Guíxols, Spain
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Fruit trees, as perennial plants, continuously face the threat of various pests and diseases, making disease resilience essential for their survival and productivity. We define resilience as the tree ability to sustain itself or quickly recover after exposure to pathogens, aiming to design ‘resilient ideotypes’ that can maintain growth and productivity despite environmental pressures. This study proposes to evaluate disease resilience in a 206 individual peach core-collection by measuring so-called resilience biomarkers, including trunk growth, flower density, and fruit load. These measurements were conducted alongside visual assessments of 7 pest and disease symptoms in low phytosanitary conditions at three locations in southeastern France during three consecutive seasons. From this large dataset, we developed a multi-disease index and a four-category resilience classification to identify peach varieties that can withstand multiple biotic stresses. Finally, we compared i) multivariate GWAS model, ii) index based GWAS and iii) PCA based multi-trait genome-wide association studies (GWAS). Our findings demonstrated the effectiveness of these biomarkers in defining resilient varieties tailored to their specific environments. Sixteen co-localizing peaks were identified, suggesting possible pleiotropic effects of genes that influence immunity against multiple pests, diseases, and resilience indices. As a result, this study offers initial insights into the genetic structure of disease resilience components in peach. By prioritizing resilience in breeding programs, we aim to develop more sustainable orchards via the use of varieties that can resist diseases while minimizing the use of pesticides.
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https://hal.inrae.fr/hal-05065683/file/T2-PS1.17_GWAS%20ON%20MULTIPLE%20TRAITS%20AND%20RESILIENCE%20INDICATORS_B.QUILOT.pdf BibTex
titre
Pyramiding of root-knot nematode resistance genes in Prunus rootstocks
auteur
Henri Duval, Cyril van Ghelder, Naima Dlalah, Laure Heurtevin, Caroline Callot, Daniel Esmenjaud
article
12th Rosaceae Genomic Conference (RGC12), May 2025, Sant Feliu de Guíxols, Spain
resume
One of the aims of our Prunus rootstock breeding program is to integrate the natural genetic resistances to root-knot nematodes in Prunus species and maximize resistance durability. We have therefore identified and characterized three major genes Ma, RMja and RMia in plum, almond and peach, respectively. While Ma and RMja are orthologs and have a peculiar sequence, RMia is quite different in terms of localization and genome structure. These three TIR-NB-LRR (TNL) genes have different and complementary spectra of resistance to different root-knot nematode species. Consequently, their combination in a single rootstock using gene pyramiding would be of great interest to sustainably control nematode populations. Therefore, we have developed a series of reliable molecular markers that can be used in breeding programs to create and assess three-way hybrid rootstocks containing all three genes.
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https://hal.science/hal-05071386/file/T2-PS1.27_PYRAMIDING%20OF%20ROOT-KNOT%20NEMATODE%20RESISTANCE%20GENES_H.Duval.pdf BibTex
titre
Disentangling different sources of peach leaf curl disease tolerance through linkage and association genetic in several peach populations
auteur
Clotilde Nigon, Naïma Dlalah, Luana Gillet, Sabrina Viret, Frédéric Gilles, Marie Serrie, Véronique Signoret, Camille Micheli, Nathalie Courthieu, Valérie Gallia, Morgane Roth, Bénédicte Quilot-Turion
article
12th Rosaceae Genomic Conference (RGC12), May 2025, Sant Feliu de Guíxols, Spain
resume
Peach leaf curl, a fungal disease caused by the ascomycete Taphrina deformans, triggers spectacular reddish tumorous deformations on the leaves and causes massive defoliations or even the death of peach trees. To prevent significant reductions in fruit production, fruit producers mostly resort to copper based phytopharmaceuticals. Although tolerant varieties do exist, recent varieties do not bear any tolerance. This trait is certainly polygenic and genetic studies and molecular tools are needed to breed tolerant varieties. Hence, the aim of this study is to identify potential different sources of tolerance to peach leaf curl disease and characterise the various loci controlling for this trait. To do so, we bet on the combination of different analyses, populations and study sites. Symptom frequencies were recorded for at least 2 years in pesticide free orchards, on 3 different peach populations: i) a peach core-collection of 206 accessions replicated in two randomized blocs planted at four locations and genotyped with 16K IRSC peach SNP array ; ii) the ‘Malo Konare’ population composed by 212 hybrids coming from a selfing of ‘Malo Konare’, a Bulgarian cultivar planted at one location ; iii) a progeny obtained from a P. davidiana x P. persica pseudo-backcross composed of 123 hybrids replicated in one to two randomized blocs at two locations. The two latter were genotyped with IPSC peach 9K SNP array v1. Multisite and multiyear genome wide association study (GWAS) is implemented on the core-collection dataset while QTL detection is performed on the progeny datasets. Several tolerance QTLs were detected. The next objectives will be i) to set up marker assisted selection methodology to support the integration of leaf curl tolerance into peach breeding programs and ii) to test the impact of accumulation of different factors on the global level of tolerance of a variety and its durability.
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Poster communications
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https://hal.science/hal-05065082/file/Poster_RGC12_Clotilde.pdf BibTex
titre
Developing potyvirus resistance in tomato and potato by targeting susceptibility factors with CRISPR-Cas9
auteur
Linping Wang, Caroline Lebaron, Katrin Artola, Jean-Luc Gallois, Kristiina Makinen
article
International Advances in Plant Virology 2025, Apr 2025, Murcia, Spain
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Viral diseases in crops present an escalating global threat to food security, with potyviruses being particularly problematic. Genome editing offers new opportunities to introduce genetic resistance in crops of interest, and while the eukaryotic translation initiation factor 4E (eIF4E) is commonly studied for enhancing resistance in crop breeding (Kuroiwa et al., 2022), there is an urgent need for novel targets. Our research investigates the VARICOSE (VCS) gene, a susceptibility factor with a proviral effect for potyvirus infection. Our findings indicated that VCS facilitates systemic viral spread and particle formation by associating with the viral helper component proteinase (HCPro) (De et al., 2020). As such, VCS could be a new target to develop genetic resistances. However its inactivation is likely to cause strong developmental defects, which would jeopardize its use in plant breeding. Here, we aim at taking advantage of the presence of two VCS genes in tomato and potato to find a trade-off between resistance to potyviruses and plant development. Using CRISPR-Cas9 genome editing, we initiated an experiment to test if we can develop virus-resistant tomato and potato plants by knocking out the two VCS genes separately or both together. The resulting plants will be analysed for both resistance to potyvirus and for development. We will also aim at pyramiding VCS knock out with eIF4E resistance to see if this could help improving the plant resistance spectrum or resistance durability. This study is expected to provide valuable insights into potyvirus resistance mechanisms and some information to guide future strategies for durable crop protection
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Strategies for developing new genetic resistances to viruses based on the susceptibility factors phosphoglycerate kinases
auteur
Geoffroy Milkoff, Lucas Laflaquière, Nathalie Giovinazzo, Mamoudou Diop, Zoé Terret-Welter, Cécile Labourey, Jean-Luc Gallois
article
20es Rencontre de Virologie Végétale, Jan 2025, Aussois, France
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Plant genes encoding susceptibility factors are good targets to develop genetic resistances to viruses. Although resistances to potyviruses mainly rely on translation initiation factors such as eIF4E, it is important to characterize new host susceptibility factors. In this light, we investigate the role of the metabolic enzymes Phosphoglycerate kinases, which have been shown to act as susceptibility factors to several potyviruses. We show that inactivation of cPGK2, that has been characterized as a natural resistance allele in the Arabidopsis accession Cvi, is not sufficient to trigger resistance to watermelon mosaic virus. We reasoned that this could be caused by the existence of a gene closely homolog to cPGK2 - cPGK1- that could also be targeted by the virus. Interestingly, cPGK1 accumulates predominantly over cPGK2 in leaf cells, suggesting that knocking out cPGK2 should not be sufficient to lower the level of PGK susceptibility factors. Moreover, significantly lower levels of cPGK1mRNA accumulates in the Cvi accession: the natural resistance in Cvi could simultaneously result from a mutated cPGK2 and a lower abundance of cPGK1. We posit that Arabidopsis is a good model to understand the complexity of cPGK based susceptibility/resistance, with the aim of developing effective resistances without altering plant development. Based on this hypothesis, I will try to mimick the Cvi natural resistance to potyviruses in a susceptible Arabidopsis thaliana accession, by a combination of endogenous PGKinactivation and genetic complementation. In parallel, I will investigate the mechanisms by which the plant PGKs are recruited by the virus. Finally, I will present strategies to transfer in Tomato PGK-based resistance to poty- and potexviruses using genome editing techniques. Overall, this work should provide guidelines for designing resistance based on S genes families in crops.
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Attempts at translating resistance to potexvirus from Arabidopsis to tomato by inducing loss-of-susceptibility
auteur
Caroline Lebaron, Jean-Luc Gallois
article
20es rencontres de Virologie Végétale, Jan 2025, Aussois, France
resume
A major goal of plant breeding is to translate genetic resistances to viruses across species and in the genetic background of choice. In the case of susceptibility factors, resistance to viruses can be achieved by genome editing following the inactivation (knock-out) or modification of Susceptibility (S) genes, but how these mechanisms can be translated from model plant to crops is not always clear. Moreover, for breeding purposes, resistance should not be developed at the expense of the plant development. Here, we focus on two S genes involved in resistance to potexvirus, namely nCBP (novel Cap Binding Protein), encoding a protein related to the family of eIF4E translation initiation factors, and EXA1 (Essentials for poteXvirus Accumulations 1), which encodes a GYF domain-containing protein. It has been previously shown that i) inactivating nCBP or EXA1 in Arabidopsis limit the viral propagation of the potexviruses PlAMV (Hashimoto et al. 2016, Plant J) and that ii) EXA1 and eIF4E family members play additive roles on the accumulation of Potexvirus (Nishikawa et al. 2023 J Virol.). Here, to translate those results to crop, we describe the production of ncbp and exa1 mutants in different tomato genetic backgrounds, using genome editing techniques. We will present preliminary results on resistance to viruses as well as effect of the mutations on the plant and fruit development. This might provide useful insight on the transferability of those S genes for breeding purposes, as well as information on the trade-off between resistance and development.
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2024

Conference papers

titre
Integrating time in GWAS pipelines to better understand genetic resistances to bacterial wilt
auteur
Adrien Belny, Richard Berthomé, Elise Albert, Laurent Grivet, Céline Labourey, Grégori Bonnet, Laurence Moreau, Tristan Mary-Huard
article
ENVIE, Impact of Environment on plant immunity and pathogen virulence, Nov 2024, SETE, France., Richard Berthomé; Mathilde Fagard; Valerie Schurdi-Levraud; Chloé Delmas; Frederic Domergue; Marie Foulogne-Oriol, Nov 2024, Bordeaux, France
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Phénotypage haut-débit de la diversité génétique des arbres fruitiers pour une meilleure adaptation au changement climatique (PHADA) - Segmentation des parties boisées
auteur
Khac Lan Nguyen, Marie Weiss, Sylvain Jay, Frédéric Boudon, Evelyne Costes, Morgane Roth
article
1ère Journée thématique sur le deep learning (DL), Réseau 2Neurones, Oct 2024, Nantes, France
resume
Le phénotypage joue un rôle essentiel dans le développement de variétés végétales capables de s'adapter aux conditions climatiques changeantes. Un phénotypage précis et fiable permet de mieux comprendre les mécanismes génétiques à l'origine de l'expression des traits (comme le diamètre du tronc, la floraison ou l’architecture de l’arbre), facilitant ainsi le développement de marqueurs moléculaires utilisables dans la sélection variétale. Dans le cas du phénotypage des arbres fruitiers, les mesures manuelles de traits sur un grand nombre d’arbres sont lentes et fastidieuses et peuvent manquer d’objectivité. Des outils de phénotypage numérique sont actuellement développés dans le cadre du projet PHADA pour remédier à cette limitation. Ces outils sont basés notamment sur des images RVB (Rouge-Vert-Bleu) d’arbres, acquises en visée latérale par stéréovision, et sur des méthodes d’apprentissage profond pour estimer les traits à partir des images. Le premier objectif du projet est d’extraire les parties boisées situées au premier plan à partir d’images RVB acquises en verger, en exploitant ou non l’information de profondeur disponible grâce à la stéréovision. Deux modèles d’apprentissage profond basés sur l’architecture UNet ont été utilisés pour segmenter les parties boisées des arbres situés au premier plan dans les images. Une base d’images annotées incluant une diversité d’espèces, d’années et de sites d’étude a été collectée, afin d’entraîner ces modèles. Dans cette étude, 1151 patches RVB ont été utilisés pour l'entraînement, 163 patches pour la validation et 30 patches pour le test. Parmi ces patches, l’information de profondeur, obtenue via la méthode d’apprentissage profond IGEV, était disponible pour seulement 36 % des images utilisées pour l'entraînement, 23 % des images utilisées pour la validation et la totalité des images de test. Un premier modèle (modèle RVB) a été entraîné à partir des seules images RVB, c’est-à-dire, sans exploiter l’information de profondeur. Le deuxième modèle (modèle RVB_P) a été entraîné en simulant, sur les images stéréos disponibles, l’effet d’un flash à partir de l’image de profondeur. Le modèle 1 a été évalué sur les 30 patches de test non transformés, tandis que le modèle 2 a été évalué sur les mêmes patches après simulation du flash. Le modèle RVB a obtenu un score F1 de 0.92 sur les 30 patches de test non transformés, tandis que le modèle RVB_P a atteint un score F1 de 0.89 sur les 30 patches transformés. Une analyse détaillée des résultats de segmentation révèle que le modèle RVB_P est toutefois supérieur au modèle RVB grâce à l'apport de l’information de profondeur, notamment pour les patches contenant plusieurs rangs d’arbres. En effet, le modèle RVB_P permet d’éliminer quasiment la totalité des arbres situés en arrière-plan, contrairement au modèle RVB. Toutefois, ce dernier est plus performant que RVB_P pour segmenter les rameaux fins, ceux-ci étant moins bien détectés par l’algorithme stéréo IGEV. L’utilisation d’une méthode alternative d’estimation de la profondeur, comme Marigold, est envisagée pour résoudre ce problème.
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https://hal.inrae.fr/hal-04829626/file/Presentation_Nantes_NGUYEN_MW.pdf BibTex
titre
Arabidopsis eIF4E1 protects the translational machinery during TuMV infection and restricts virus accumulation
auteur
Delyan Zafirov, Nathalie Giovinazzo, Cécile Lecampion, Ben Field, Julia Novion Ducassou, Yohann Couté, Karen S Browning, Christophe Robaglia, Jean-Luc Gallois
article
Post-transcriptional Gene Regulation in Plants 2024, Sep 2024, Banyuls Sur Mer, France
resume
Successful subversion of translation initiation factors eIF4E determines the infection success of potyviruses, the largest group of viruses affecting plants. In the natural variability of many plant species, resistance to potyvirus infection is provided by polymorphisms at eIF4E that renders them inadequate for virus hijacking but still functional in translation initiation. In crops where such natural resistance alleles are limited, the genetic inactivation of eIF4E has been proposed for the engineering of potyvirus resistance. However, recent findings indicate that knockout eIF4E alleles may be deleterious for plant health and could jeopardize resistance efficiency in comparison to functional resistance proteins. Here, we explored the cause of these adverse effects by studying the role of the Arabidopsis eIF4E1, whose inactivation was previously reported as conferring resistance to the potyvirus clover yellow vein virus (ClYVV) while also promoting susceptibility to another potyvirus turnip mosaic virus (TuMV). We report that eIF4E1 is required to maintain global plant translation and to restrict TuMV accumulation during infection, and its absence is associated with a favoured virus multiplication over host translation. Furthermore, our findings show that, in the absence of eIF4E1, infection with TuMV results in the production of a truncated eIFiso4G1 protein. Finally, we demonstrate a role for eIFiso4G1 in TuMV accumulation and in supporting plant fitness during infection. These findings suggest that eIF4E1 counteracts the hijacking of the plant translational apparatus during TuMV infection and underscore the importance of preserving the functionality of translation initiation factors eIF4E when implementing potyvirus resistance strategies
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https://hal.inrae.fr/hal-04718732/file/PGRP2024_AbstractBook.pdf BibTex
titre
Setting the scene and formulation of expected outcomes of the joint PRO-GRACE/EMPHASIS workshop for promoting a plant genetic resources community for Europe.
auteur
Véronique Lefebvre, Ignazio Verde
article
Joint PRO-GRACE/EMPHASIS policy symposium and workshop about plant genetic resources and phenotyping, PRO-FRACE European project and EMPHASIS information system, Jun 2024, Brussel, Belgium
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À la recherche d’arbre fruitier résilients pour réduire l’utilisation des pesticides dans les vergers
auteur
Marie Serrie
article
Tersys Implanteus 3rd Summer School (TISS3), SFR Tersys; EUR Implanteus, Jun 2024, Avignon, France
resume
Les vergers fruitiers sont confrontés à l’attaque de multiples bioagresseurs qui sont responsables d’importants dégâts. Malgré la demande urgente de réduction des produits phytosanitaires, les pesticides demeurent le moyen de lutte le plus efficace. Une solution prometteuse pour répondre à cette demande repose sur l’utilisation de la diversité génétique naturelle pour créer des variétés dites ‘résilientes’, c’est-à-dire peu affectées par des perturbations extérieures ou qui retrouvent rapidement leur état de production initial après l’exposition à des stress combinés. Cette étude vise à définir et à caractériser les composantes phénotypiques et génétiques de la résilience chez les Prunus. Pour ce faire, nous avons déployé et maintenu en conditions phytosanitaires réduites deux core-collections de respectivement 150 et 206 accessions d’abricotier et de pêcher dans cinq lieux différents. À travers un suivi fin et intégratif des multiples symptômes de bioagresseurs au fil des années, nous avons décrypté les différentes manifestations phénotypiques de la résilience. Différents modèles de génétique d'associations ont été testés afin d'identifier les marqueurs génétiques liés aux composantes de la résilience. Cette étude représente une première étape indispensable de compréhension des mécanismes de résilience et contribue à une modification profonde et nécessaire des objectifs de création variétale pour tendre vers des systèmes agricoles résilients.
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Determining leaf biochemical and phytosanitary status of orchard trees with spectroscopy from visible to long-wave infrared range
auteur
Karine Adeline, Nathan Sikora, Jean-Pierre Segaud, Sylvain Jay, Jean-Baptiste Féret, Sylvie Serino, Doriane Dumont, Gilles Vercambre, Marie-Noëlle Corre, Morgane Roth
article
25èmes Rencontres Héliospir, Jun 2024, Montpellier, France
resume
The use of spectroscopy data from the visible to short-wave infrared range has proven to be efficient to quantify relevant biochemical traits at leaf scale related to photosynthetic activity, water content, biomass and nutrient allocation, as well as disease stage. We propose to extend this work by (i) completing the spectral analysis with the mid- and long-wave infrared domain, (ii) studying the estimation accuracy and the method robustness by assessing a large panel of different observations, and (iii) deriving biochemical and phytosanitary variables from the same samples. From two INRAE orchard experimental sites located respectively at Avignon (Vaucluse) and Bellegarde (Gard), shoots comprising at least seven leaves were collected from peach trees (Nectarlove cv) undergoing two contrasted fertilization trials (ECOPECHE network), and from apricot trees undergoing low-input treatment with replications of different varieties (CORE COLLECTION network). This data collection was repeated each month from June to October 2023, and shoots were sampled for each tree on the bottom and top parts of the crown (different nutrient/water availability and illumination condition). Leaf directional-hemispherical reflectance and transmittance were measured with integrating spheres coupled with spectroradiometers (Perkin Elmer Lambda 950 instrument for 400 – 2500 nm and Bruker Invenio X for 2500 – 15000 nm). Biochemical traits were also measured, such as leaf-clip SPAD, fresh and dry weight, and leaf dimensions. At shoot scale, pigments (phenolics compounds, chlorophylls and carotenoids) and total nitrogen contents were analyzed in laboratory. We used correlation regressions with SPAD to upscale these data at leaf scale. Scoring of the phytosanitary status quantifying chlorosis, leafhopper attack, and the presence of shot holes and rust, was visually assessed at both leaf and shoot scales by an expert. In total, 155 samples were available. Three methodological concepts to estimate vegetation biochemical and phytosanitary variables are explored: (1) an empirical approach by using the partial least squares regression (PLSR) to estimate all variables, (2) a physical approach based on the iterative inversion of the leaf radiative transfer model PROSPECT to estimate leaf biochemistry (pigments, water and dry matter contents), and (3) a hybrid approach combining both previous ones. For spectra from the visible to short-wave infrared range, all three approaches will be compared while for those from mid- to long-wave infrared range, only the empirical approach will be explored. Results are highlighted in function of tree species and variety, the phenological stage, the crown vertical sampling and fertilization trials. This study aims in assessing agro-ecological practices (ex: phytosanitary use reduction, resilient varieties breeding) from nondestructive spectroscopic proximal sensing measurements giving access to vegetation status. The prospects include the upscaling of these results to outdoor conditions with unmanned aerial vehicle and terrestrial acquisitions at centimetric spatial resolution to target the leaf scale and observe the intra-individual variability inside a tree crown. This work is funded by the project ANR CANOP (ANR-22-CE04-0002).
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Assessing Flowering Intensity of Diverse Malus and Prunus Varieties: A Comparative Analysis of Aerial and Terrestrial Based Stereo Images
auteur
Nathan Guilhot, Jeremy Labrosse, Isabelle Farrera, Guillaume Perez, Florence Meunier, Morgane Roth, Mathias Lefèbvre, Marc Labadie, Sylvain Jay, Marie Weiss, Evelyne Costes, Frédéric Boudon
article
European Horticulture Congress, May 2024, Bucarest, Romania
resume
Assessing flowering intensity in orchards is crucial for effective tree management, yield optimization and to forecast adaptation of varieties in the context of climate change. Flowering intensity is influenced by genetic and environmental factors. For now, the traditional methods to assess flowering intensity are based on visual observation and are tedious and qualitative.

Our aim was to develop an image analysis pipeline to estimate as accurately and efficiently as possible the flowering intensity and compare it to direct observations. Data were acquired on Malus (apple) and Prunus (apricot and peach) species, which exhibit differences in phenology and within-tree flower position that might necessitate adaptation for the assessment of their flowering intensity. We also conducted a comprehensive comparative analysis between two data acquisition protocols: aerial and terrestrial based stereo imagery providing top and lateral views of the trees respectively. They were tested on core-collections of apple and prunus located at INRAE experimental units in Montpellier and Avignon (France), respectively.For aerial based imagery, we employed photogrammetry to generate a colored point cloud. From the terrestrial based imagery, we extracted depth maps using stereovision. In both cases, we focused on the central canopy area to minimize interference with neighboring trees. Semantic segmentation was employed to identify pixels or points corresponding to each tree from the two types of data. We estimated the tree volume and performed a flower identification, using either color filters or semantic segmentation.

Promising results were obtained by comparing the predictions from the pipeline with expert ground truth and heritability values. Subsequently, we examined the advantages and limitations of aerial and terrestrial based stereo imagery in assessing flowering intensity. This exploration presents interesting perspectives for high throughput phenotyping of flowering fruit tree collections of diversity.

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https://hal.science/hal-04734120/file/Guilhot%20et%20al.%20EHC2024_S08_vf.pdf BibTex
titre
Comparative Analysis of Aerial and Terrestrial Imaging Techniques for Assessing Flowering Intensity of Diverse Malus Varieties
auteur
Nathan Guilhot, Jérémy Labrosse, Isabelle Farrera, Guillaume Perez, Florence Meunier, Morgane Roth, Mathias Lefèbvre, Marc Labadie, Sylvain Jay, Marie Weiss, Evelyne Costes, Frédéric Boudon
article
5th European Horticulture Congress (EHC 2024), University of Agronomic Sciences and Veterinary Medicine of Bucharest; Romanian Society of Horticulturists, May 2024, Bucharest, Romania
resume
Assessing flowering intensity in orchards is crucial for effective tree management, yield optimization and to forecast adaptation of varieties in the context of climate change. Flowering intensity is influenced by genetic and environmental factors. For now, the traditional methods to assess flowering intensity are based on visual observation and are tedious and qualitative. Our aim was to develop an image analysis pipeline to estimate as accurately and efficiently as possible the flowering intensity and compare it to direct observations. Data were acquired on Malus domestica (apple) species, which exhibit differences in phenology and within-tree flower position that might necessitate adaptation for the assessment of their flowering intensity. We also conducted a comprehensive comparative analysis between two data acquisition protocols: aerial and terrestrial based stereo imagery providing top and lateral views of the trees respectively. They were tested on core-collections of apple and prunus located at INRAE experimental units in Montpellier and Avignon (France), respectively. For aerial based imagery, we employed photogrammetry to generate a colored point cloud. From the terrestrial based imagery, we extracted depth maps using stereovision. In both cases, we focused on the central canopy area to minimize interference with neighboring trees. Semantic segmentation was employed to identify pixels or points corresponding to each tree from the two types of data. We estimated the tree volume and performed a flower identification, using either color filters or semantic segmentation. Promising results were obtained by comparing the predictions from the pipeline with expert ground truth and heritability values. Subsequently, we examined the advantages and limitations of aerial and terrestrial based stereo imagery in assessing flowering intensity. This exploration presents interesting perspectives for high throughput phenotyping of flowering fruit tree collections of diversity.
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https://hal.inrae.fr/hal-04832117/file/Guilhot%20et%20al_revised_EHC.pdf BibTex
titre
Investigating the multi-disease challenge in apricot through single and multienvironment genome wide association studies
auteur
Marie Serrie, Vincent Segura, Alain Blanc, Laurent Brun, Freddy Combe, Amandine Fleury, Mathilde Le Pans, Véronique Signoret, Sabrina Viret, Jean-Marc Audergon, Bénédicte Quilot-Turion, Morgane Roth
article
1. International ISHS symposium on Apricot and Plum, INRAE PACA - GAFL (UR1052 GAFL Génétique et amélioration des fruits et légumes), Apr 2024, Avignon, France
resume
Over their entire lifetime, apricot trees are exposed to a wide range of pests and diseases incurring for significant sanitary impacts and economic losses. Several sources of partial resistances have been identified but the underlying genetic architecture has yet to be elucidated for most diseases. In this study, the objective is to identify the genetic components underlying resistance – or low susceptibility – of two major diseases in apricot: blossom blight (Monilinia spp.) and leaf rust (Tranzschelia spp.). To do so, a core collection composed by 150 accessions replicated in 5 randomized blocs was grown under low phytosanitary conditions in two environmentally contrasted locations in South-East of France. These accessions have been densely sequenced with the Illumina HiSeq 2000 NGS technique. After monitoring rust and blossom blight damages from 2020 to 2023, we dissected the observed phenotypic variation into genotype, environment and their interactions effects via descriptive statistics and variance decompositions. To identify genetic markers linked to resistance components, we firstly performed genome wide association studies (GWAS) for each environment separately. We found stable genetic effects across the environment, reflected by robust quantitative trait loci (QTLs). In contrasts, some QTLs were only detected in a specific set of trial. To jointly analyse site-specific GWAS results and improve statistical power, we used a meta-analysis GWAS approach. By using a random effect procedure, we were able to consider the heterogeneity of the QTL effects across environments and the correlation between the single-environment GWAS. In the last approach we performed multi-environment GWAS with the MTMM package to dissociate QTLs from QTL by environment interactions effects. The present results provide insights into the genetic basis of blossom blight and rust susceptibility in apricot and therefore contribute to the development of genomics-assisted breeding to improve biotic resilience in apricot varieties.
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Investigating the genetic mechanisms of resilience in apricot and peach to cope with the multiple disease challenge in orchards
auteur
Marie Serrie
article
Doctoriales 2024 - Avignon Université, Avignon Université, Apr 2024, Avignon, France
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Elucidating the Genetics of Fruit Quality Traits in a Pepper Core Collection
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Yury Tikunov, Yi Wu, Giorgio Tumino, Pasquale Tripodi, Véronique Lefebvre, Ilan Paran, Richard G.F. Visser, Arnaud Bovy
article
Plant and Animal Genome XXXI Conference, Jan 2024, San diego, CA, United States
resume
The Horizon 2020 G2PSOL project aimed at characterizing the genetic diversity in genebank accessions of 4 Solanaceous crop species, tomato, pepper, eggplant and potato. Based on a low density GBS analysis of 10.301 pepper genebank accessions, a core collection of 400 C. annuum accessions, representing the genetic variation in this Capsicum species, was selected for high density genotypic analysis and broad phenotypic characterisation in trials at different locations (McLeod et al, 2023). Fruit metabolite content was determined in two trials, conducted in France and Italy, and included specialized metabolites, such as flavonoids, carotenoids, casaicinoids and flavour-related volatiles. GWAS analysis revealed the presence of a strong major QTL for flavonoids on chromosome 5. The most significant SNP was located in the CaMYB12-like gene, which was previously demonstrated as the causal gene underlying flavonoid variation in mapping populations segregating for flavonoid content, by VIGS analysis and by complementation studies in tomato (Wu et al. 2023). In addition, several other mQTLs were identified for carotenoids, capsaicinoids and flavour volatiles. The pepper core collection was also characterized for variation in postharvest water loss, an important determinant of shelf life in pepper fruit. GWAS analysis revealed several significant QTLs associated with water loss characteristics and these are currently being validated in segregating populations.
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Poster communications

titre
Whole genome sequencing and epigenotyping for multiple generations of selection using Oxford Nanopore Technology
auteur
Sonia E Eynard, Cécile Donnadieu, Loïc Flatres-Grall, Carole Iampietro, Sandrine Lagarrigue, Sophie Leroux, Joana Lledo, Marie-José Mercat, Juliette Riquet, Céline Vandecasteele, Frédérique Pitel, Bertrand Servin
article
International congress in Ecology and Evolution, Oct 2024, Lyon, France
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Centre de Ressources Biologiques CRB-Lég INRAE -PACA
auteur
Manon Bouët
article
Journées scientifiques du Dép. BAP, Sep 2024, 27130 Verneuil-sur-Avre, France, Sep 2024, Verneuil-sur-Avre, France
resume
Activités principales, fonctionnement et caractéristiques du CRB Légumes
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https://hal.science/hal-04974184/file/2024_%20TRAMEPOSTER-CRB_Leg_20240917_V2.pdf BibTex
titre
Graph pangenome of eggplant and (its) allied species
auteur
Luciana Gaccione, Marie Bolger, Laura Toppino, Ezio Portis, Sergio Lanteri, Giuseppe Leonardo Rotino, Maria Rosaria, Jaime Prohens, David Alonso, Giuseppe Aprea, Paola Ferrante, Véronique Lefebvre, Emily Idahl, Maximilian Schmidt, Hatice Filiz Boyaci, Giovanni Giuliano, Björn Usadel, Lorenzo Barchi
article
67th annual congress of the Italian Society of Agricultural Genetics, Sep 2024, Bologna, Italy
resume
Recent advances in sequencing technologies and the reduction of their costs have accelerated the assembly of plant genomes. However single reference genomes do not fully capture the species diversity, leading to the development of the pangenome concept. A pangenome encompasses all DNA sequences available for a species or larger taxa, including single nucleotide polymorphisms (SNPs) and small/large structural variants (SVs). To better characterize these variations, the development of graph-based pangenomes have emerged. They store sequence and SV information in nodes and paths, thereby expanding the linear reference genome's coordinate system to more effectively capture genetic diversity. In this study, Oxford Nanopore long reads from 40 accessions, including 33 accessions of Solanum melongena among which the reference line ‘67/3’, as well as 7 accessions of the allied species S. insanum and S. incanum were assembled using NextDenovo. The resulting assemblies were polished with Medaka and chromosome-scale assemblies were generated for six S. melongena and two accessions each of S. insanum and S. incanum, by incorporating chromosome conformation capture (Hi-C) data. The chromosomes of the other 30 accessions were reconstructed using RagTag, based on the reference line ‘67/3’ sequence. A combination of Helixer (Deep Neural Networks ab initio gene prediction) and Braker-3 (both ab initio and evidence-based gene prediction) was applied to annotate each genome, resulting in a gene set ranging from 30,886 to 33,449, with a BUSCO completeness from 95.3 to 97.2%. The obtained assemblies were used to build up two different graph-based pangenomes. The first one, which includes the 40 accessions, was built using the PanGenome Graph Builder (PGGB) pipeline. The second one, comprising only the 33 S. melongena accessions, was obtained with Minigraph-Cactus pangenome pipeline. The latter was then used for genotyping 311 S. melongena accessions from the EU G2P-SOL project core collection, which were sequenced with Illumina short read sequencing. Overall, 4,532 high-quality SVs were identified. The accession panel was phenotyped, among others, for resistance to fungal wilts at two sites: Montanaso Lombardo (Italy), and Antalya (Türkiye), and associations between phenotype and genotype were identified using BLINK model within GAPIT R package, on multi-environment data. Here, we report the genome-wide association (GWA) results for resistance to Fusarium oxysporum f. sp. melongenae and Verticillium dahliae. Our results highlight that the graph pangenome provides an efficient model for representing and analyzing multiple genomes and their variations. This offers a comprehensive resource for eggplant breeding programs and enhances the understanding of eggplant genetic architecture of traits, particularly in relation to fungal wilt resistance.
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https://hal.inrae.fr/hal-04670168/file/Gaccione_et_al_graph_pangenome_Unito_SIGA_2024_Italy.pdf BibTex
titre
Towards a tool to support the design of plant protection solutions in the context of fruit growing
auteur
Julie Borg, Mohamed-Mahmoud Memmah, Jean-Noël Aubertot, Philippe Blanc, Stéphanie Drusch, Pascal Borioli, Danièle Bevacqua, Valérie Gallia, Bertrand Gauffre, Hélène Gautier, Michel Génard, Isabelle Grechi, Pierre P. Franck, Baptiste Labeyrie, Claire Lavigne, Françoise Lescourret, Vincent Mercier, Christophe Mouiren, Didace Ndalla Landou, Frédéric Normand, Marie-Hélène Robin, Antoine Rolland, Julien Ruesche, Natacha Sautereau, Pierre Valsesia, G. Vercambre
article
18th Congress of the European Society for Agronomy (ESA), Aug 2024, Rennes, France. pp.415-416, Book of abstract - 18th Congress of the European Society for Agronomy
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Using non-addive effects in genome-wide association studies and genomic predictions to improve biotic stress tolerance in peach
auteur
Morgane Roth, Marie Serrie, Vincent Segura, Naïma Dlalah, Octave Cabel, Lucas Malbot-Calonnec, Bénédicte Quilot
article
7. International Conference of Quantitative Genetics (ICQG7), Jul 2024, Vienna, Austria.
resume
Accounting for genetic architecture is crucial to breed for sustainable disease resistances and tolerances in plants. Indeed, (i) harnessing together minor and major effects genes allows to design a more durable plant immunity with large spectrum (ii) access to non-additive variance allows for a better exploitation of the total genetic variance when it comes to breeding, which is particularly relevant for clonally propagated crops. Our study system, Prunus persica (peach tree), is a major temperate fruit crop characterized by an overall high susceptibility to several pests and diseases, illustrated by a frequency treatment index around five times higher than in cereals. In this work, we phenotyped symptoms of two pests (leafhopper and twig moth) and four diseases (rust, leaf curl, mildew and shot hole) under low pesticide cover over three years in a peach core-collection replicated at three sites. This population consists in 192 unique accessions representing peach worldwide diversity and has been genotyped with the IRSC 16K SNP array. We used linear mixed models and the natural orthogonal interactions approach (Vitezica et al. 2017) to explicitly decompose genetic variance into additive, dominant and epistatic effects, and genotype x environment interactions. Genome-wide associations studies (GWAS) were performed with single-locus mixed models including kinships accounting for different dominance inheritance patterns. Genomic predictions consisted in a comparison of five GBLUP models incorporating different combinations of non-additive and inbreeding effects. After describing significant non-additive genetic variance and inbreeding effects across traits, we show that in addition to additive quantitative trait loci (QTLs), three to eight additional QTLs have been detected when accounting for dominant architecture. We were also able to improve genomic predictions by up to +0.05 in predictive ability with models incorporating non-additive and inbreeding terms in comparison to the additive baseline GBLUP model. Our results indicate the presence of very contrasted genetic architectures within the six biotic stress responses studied, traits being more strongly influenced either by dominance, epistasis or inbreeding effects. We also present the exploitation of GxE variance to find robust QTLs and improve environment-specific predictions. Finally, we introduce a multitrait approach to exploit jointly the complementary between resistance and tolerance to several biotic stresses. Our results could translate into high genetic gain in peach given its long juvenile phase, and could contribute to a long-term reduction of pesticide reliance in fruit production.
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https://hal.science/hal-04667817/file/ICQG_24_Final.pdf BibTex
titre
Whole genome exon-capture: a targeted approach for addressing apricot genetic diversity on suitable bases for genetic analysis
auteur
Laure Heurtevin, Jacques Lagnel, Frédérique Bitton, Sylvain Santoni, Christine Tollon-Cordet, Audrey Weber, Jean-Marc Audergon
article
ISHS plum and apricot 2024, Apr 2024, Avignon, France. ⟨10.1371/journal.pone.015460⟩
resume
Throughout domestication processes, phenotypic and genetic differentiations have occurred both under environment and human selection pressures. Clear phylogeographic structuration has been stated in apricot, and the challenge for the breeders is to fine tune tools capable of valorizing the available phenotypic and genetic variability both for methodological studies and selection purposes. Micro-array have been developed for peach, peach-related species and cherry, but none are available on plum and apricot. Exon capture strategy has been implemented to give the opportunity to target genes and regions of interest in the genome. Targeted capture provides an efficient and sensitive tool to access genome exons polymorphism in a high-throughput manner. Two approaches were used: one by designing one or two capture probes (in 5’and 3’ UTR of genes) , called baits, on all 37521 Prunus armeniaca Marouch v3 genes (Groppi A et al, 2021) and the other by design ten baits distributed over the coding regions of targeted candidate genes (1981 candidats genes) already described in the prunus literature. A total set of 60,000 80-base baits has been obtained.Initially, the set of baits has been tested on two complementary gene-pools of fourty-eight accessions, one representing apricot genetic diversity and the other recombinants lines. The results were completely in line with the expectations express a huge level of polymorphism suitable for genetic diversity analysis, and biparental population analyses and also suitable for genomic selections studies.
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https://hal.science/hal-05051467/file/poster_capture_apricot.pdf BibTex
titre
Assessing the role of the mirabelle plum (P. domestica subsp. syriaca) in sharka epidemics caused by the plum pox virus in northeastern France
auteur
Marie Brevet, Guillaume Roch, Margaux Champagne, Marion Delame, Quentin Hoffmann, Hervé Bentz, Patrice Duvivier, Sabrina Viret, Gaël Thébaud, Sylvie Dallot
article
Ist International Symposium on Apricot and Plum, Apr 2024, Avignon, France
resume
The Grand Est region is the leading French region for the production of damson (Prunus domestica subsp. insititia) and mirabelle (Prunus domestica subsp. syriaca) plums. Damson production is threatened by sharka, caused by the plum pox virus (PPV) spreading in this region, and control measures based on visual inspection and removal of symptomatic trees are implemented in orchards. In contrast, only very few symptoms have been reported on mirabelle leaves and fruits. Therefore, sharka is generally not surveyed on this species, despite the lack of scientific evidence on the susceptibility of mirabelle to the main PPV strains (M, D and Rec) and on its potential role in sharka epidemics occurring in the Grand Est region. We combined orchard surveys with experimental assays under controlled conditions to (i) estimate PPV prevalence in mirabelle orchards and (ii) evaluate the susceptibility of two mirabelle cultivars (P1725 and P1510) to PPV-M, PPV-D and PPV-Rec isolates after graft- and aphid-inoculation in comparison with a susceptible damson cultivar (P3066). PPV presence was assessed by DAS-ELISA in 7102 leaf samples collected from 4481 trees in 53 mirabelle orchards. PPV was detected in 15% of the orchards, but the overall prevalence was very low (1.7%). The virus was detected mainly in rootstock suckers (2.6%) and occasionally in asymptomatic mirabelle leaves (0.25%). Experiments showed that both of the tested mirabelle cultivars were susceptible to the three main PPV strains regardless of the inoculation method, with typical PPV symptoms on leaves. Key parameters of the infection cycle (transmission rate to test plants, incubation period, viral load, and retransmission rates by aphids from PPV-infected plants) showed that the susceptibility of the mirabelle cultivars is lower than that of the damson cultivar and depends on the PPV strain. Apparent discrepancies between survey and experimental results are discussed
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https://hal.inrae.fr/hal-04810917/file/Dallot-abstract%20ISHS2024%2012032024%20.pdf BibTex
titre
Measuring trunk circumference and flower density of trees using RGB cameras in stone fruit orchards
auteur
Morgane Roth, Sylvain Jay, Marie-Pia D'Argaignon, Nathan Guilhot, Evelyne Costes, Frédéric Boudon, Mathias Lefèbvre, Julien Desforges, Jeremy Labrosse, Marc Labadie, Marie Weiss
article
I International Symposium on Apricot and Plum, Apr 2024, Avignon, France
resume
Digital phenotyping has the potential to sustain the use of genetic resources for fruit tree breeding by increasing the throughput and the accuracy of phenotypic measurements. The aim of this study is to assess the potential of RGB cameras to measure trunk circumference and flower density in apricot and peach. Two vectors were employed to this aim: the ground-based phenotyping pole LITERAL equipped with two RGB cameras, and an unmanned aerial vehicle (UAV) AUTEL Evo II equipped with one RGB camera. We screened a large diversity of peach and apricot accessions planted in a core-collection design within orchards containing 2 to 5 randomized blocs of 206 and 150 genotypes, respectively. For estimating trunk circumference, we used pictures from LITERAL combined with wood segmentation and stereovision algorithms. Although correlation between observed and predicted values for trunks between 10 and 40cm circumference were high (r2= 0.66), trunk estimation errors were comprised between 9.5 and 13.5mm which is insufficient to measure interannual growth. Further analyses revealed that this lack of precision essentially stems from wood segmentation errors which can be addressed by improving the training set. Flower density was estimated either with single flower detection (LITERAL images) or with photogrammetric analyses followed by flower and wood segmentation (UAV images). We discuss the precision and feasibility of these measurements and our strategy to produce analytic pipelines in order to deploy digital phenotyping for trunk circumference and flower density traits which are of major interest for breeding.
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https://hal.science/hal-04776574/file/Poster-scientifique-800x1200mm_ISHS_PlumApricot2024_MRoth_v2.pdf BibTex

Reports

titre
Méthodes d’évaluation des risques sanitaires et environnementaux et des enjeux socio-économiques associés aux plantes obtenues au moyen de certaines nouvelles techniques génomiques (NTG)
auteur
Michel Gautier, Guillaume Fried, Thaura Ghneim-Herrera, Florian Guillou, Valérie Le Corre, Charlotte Lécureuil, Youenn Loheac, David Makowski, Julie Mallet, Marianne Mazier, Morgan Meyer, François Moreau-Gaudry, Sergio Ochatt, Pierre Rougé, Paule Vasseur, Laura Maxim, Bénédicte Apouey, Luc Baumstark, Céline Bonnet, Thierry Brunelle, France Caillavet, Alain Carpentier, Thomas Coutrot, Cécile Detang-Dessendre, Serge Garcia, Julien Gauthey, Emmanuelle Lavaine, Marc Leandri, Christine Le Clainche, Youenn Loheac, Selim Louafi, Eric Plottu, Elodie Rouviere, Maïder Saint Jean, Denis Salles, Louis-Georges Soler, Léa Tardieu, Dylan Cherrier, Legrand Saint-Cyr, Emmanuelle Pic, Alexis Larousse, Mathieu Baudrin, Lucie Eyraud, Karine Fiore, Youssef El Ouadrhiri
article
Saisine n°2021-SA-0019, Anses. 2024
resume
Depuis le 1er janvier 2022, conformément à l’ordonnance n° 2021-1325 du 13 octobre 2021 et au décret n° 2021-1905 du 30 décembre 2021, l’Anses reprend les missions du Haut conseil des biotechnologies (HCB) concernant l’évaluation des risques pour l’environnement de l’ensemble des utilisations de biotechnologies en milieu ouvert, et les impacts socioéconomiques. L’Anses a été saisie dans le cadre de ces nouvelles missions par la Direction générale de la prévention des risques (DGPR) et la Direction générale de l’alimentation (DGAl) sur l’utilisation des nouvelle techniques génomiques (NTG) sur les végétaux. Les autres instances ayant repris les missions du HCB, à savoir le Conseil économique, social et environnemental (CESE) et le Comité consultatif national d’éthique (CCNE), ont également été sollicité sur cette question sur les périmètres correspondant respectivement aux questions sociétales et éthiques. Par ailleurs, il convient de noter que de nombreux acteurs institutionnels publics, organisations professionnelles et syndicales ont également produit des rapports sur les NTG. Le rapport d’expertise collective est réalisé dans le périmètre des missions de l’Anses sur les biotechnologies, incluant l’évaluation des risques sanitaires et environnementaux et les impacts socio-économiques. Il vise à éclairer les demandeurs sur ce périmètre qui couvre seulement une partie des enjeux liés à l’utilisation des NTG dans le domaine de la sélection végétale. Les questions instruites dans ce rapport sont donc limitées à ce périmètre et les conclusions devront être prises en compte uniquement dans ce cadre et mises en perspective avec les avis des autres instances sollicitées. Il convient de noter que cette expertise a été engagée avant la proposition de règlement de la Commission du 5 juillet 20231. Suite à sa publication, l’Anses a décidé de s’autosaisir afin de mener une analyse des critères définissant les plantes NTG de catégorie 1, considérées comme équivalentes aux plantes conventionnelles, exposés dans l’annexe 1 et justifiés par une note technique diffusée par la Commission européenne en octobre (Anses 2023). Cette analyse a été réalisée en parallèle et ses conclusions n’ont donc pas été prises en compte dans le présent travail d’expertise, réalisé dans le cadre du périmètre défini précédemment, qui ne fait pas la distinction entre les plantes NTG de catégorie 1 et 2.
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Preprints, Working Papers, ...

titre
Investigating the genetic architecture of biotic stress response in stone fruit tree orchards under natural infections with a multi-environment GWAS approach
auteur
Marie Serrie, Vincent Segura, Alain Blanc, Laurent Brun, Naïma Dlalah, Frédéric Gilles, Laure Heurtevin, Mathilde Le Pans, Véronique Signoret, Sabrina Viret, Jean-Marc Audergon, Bénédicte Quilot, Morgane Roth
article
2024
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The mapping and introduction of sustainable plant immunity to pests and diseases in fruit tree is still a major challenge in modern breeding. This study aims at deciphering the genetic architecture underlying resistance or tolerance across environments for major pests and diseases in peach ( P. persica ) and apricot ( P. armeniaca ). We set up a multi-environment trial (MET) approach by studying two core collections of 206 peach and 150 apricot accessions deployed under low phytosanitary conditions in respectively three and two environmentally contrasted locations in South-East of France. To capture the complex dynamics of pest and disease spread in naturally infected orchards, visual scoring of symptoms was repeated within and between 3 years, for five and two pests and diseases respectively for peach and apricot, resulting in the maximum of damage score and the AUDPC. These traits were used as phenotypic inputs in our genome-wide association studies (GWAS) strategy, and leading to the identification of: i) non-additive genotype–phenotype associations, ii) environment-shared QTLs iii) environment-specific QTLs, and iv) interactive QTLs which changes in direction (‘antagonist’) or intensity (‘differential’) according to the environment. By conducting GWAS with multiple methods, we successfully identified a total of 60 high confidence QTLs, leading to the identification of 87 candidate genes, the majority belonging to the Leucine-rich repeat containing receptors (LRR-CRs) family gene. Finally, we provided a comparative analysis of our results on peach and apricot, two closely related species. The present results contribute to the development of genomics-assisted breeding to improve biotic resilience in Prunus varieties.
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https://hal.inrae.fr/hal-04759062/file/Serrie_biorxiv_2024.pdf BibTex
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Integrative multi-environmental genomic prediction in apple
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Michaela Jung, Carles Quesada-Traver, Morgane Roth, Maria José Aranzana, Hélène Muranty, Marijn Rymenants, Walter Guerra, Elias Holzknecht, Nicole Pradas, Lidia Lozano, Frédérique Didelot, François Laurens, Steven Yates, Bruno Studer, Giovanni AL Broggini, Andrea Patocchi
article
2024
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ABSTRACT Genomic prediction for multiple environments can aid the selection of genotypes suited to specific soil and climate conditions. Methodological advances allow effective integration of phenotypic, genomic (additive, non-additive), and large-scale environmental (enviromic) data into multi-environmental genomic prediction models. These models can also account for genotype-by-environment interaction, utilize alternative relationship matrices (kernels), or substitute statistical approaches with deep learning. However, the application of multi-environmental genomic prediction in apple remained limited, likely due to the challenge of building multi-environmental datasets and structurally complex models. Here, we applied efficient statistical and deep learning models for multi-environmental genomic prediction of eleven apple traits with contrasting genetic architectures by integrating genomic- and enviromic-based model components. Incorporating genotype-by-environment interaction effects into statistical models improved predictive ability by up to 0.08 for nine traits compared to the benchmark model. This outcome, based on Gaussian and Deep kernels, shows these alternatives can effectively substitute the standard G-BLUP. Including non-additive effects slightly improved predictive ability by up to 0.03 for two traits, but enviromic-based effects resulted in no improvement. The deep learning approach achieved the highest predictive ability for three traits with simpler genetic architectures, outperforming the benchmark by up to 0.10. Our results demonstrate that the tested statistical models capture genotype-by-environment interactions particularly well, and the deep learning models efficiently integrate data from diverse sources. This study will foster the adoption of multi-environmental genomic prediction to select apple cultivars adapted to diverse environmental conditions, providing an opportunity to address climate change impacts.
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A deletion in a photosynthesis-related gene (PpPSAK) determines the anthocyanin accumulation in the mesocarp of French blood-flesh peaches
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Laure Heurtevin, Carole Confolent, Patrick Lambert, Sylvie Bureau, Thierry Pascal, Zhijun Shen, Bénédicte Quilot-Turion
article
2024
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Accumulation of anthocyanin pigments is a primary determinant of fruit quality in peach ( Prunus persica L. Batsch). The recessive blood-flesh trait (bf), identified in French cultivars has been first mapped on chromosome 4. However, the underlying gene has yet been identified. In this study, the genetic, molecular and transcriptional bases of the bf trait were investigated. Blood-flesh cultivars showed high levels of cyanidin-3-glucoside and cyanidin-3-rutinoside in the fruit mesocarp about 45–60 days after anthesis. A fine mapping coupled to a positional cloning strategy mapped the bf locus to a 80-kb interval on peach linkage group (LG) 4. The sequence polymorphism analysis of genes present in this interval allowed the identification of only one candidate gene for bf as being a deletion of 21-bp in exon 3 of a PSAK-like gene. A specific marker of the deletion was developed and validated in various genetic backgrounds. An expression study of PpPSAK as well as structural, regulatory and photosynthesis related genes was performed on a kinetic from 60 days after blooming up to fruit maturity. Overexpression of PpPSAK in flesh and midrib of bf fruit was correlated with expression of anthocyanin biosynthesis structural genes. Interactions between proteins were observed and open discussion on functional pathways.
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Nanopore adaptive sampling to identify the NLR-gene family in melon (Cucumis melo L.)
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Javier Belinchon-Moreno, Aurelie Berard, Aurelie Canaguier, Veronique Chovelon, Corinne Cruaud, Stéfan Engelen, Rafael Feriche-Linares, Isabelle Le-Clainche, William Marande, Vincent Rittener-Ruff, Jacques Lagnel, Damien Hinsinger, Nathalie Boissot, Patricia Faivre Rampant
article
2024
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Nanopore Adaptive Sampling (NAS) offers a promising approach for assessing genetic diversity in targeted genomic regions. Herein, we design and validate an experiment to enrich a set of resistance genes in several melon cultivars as a proof of concept. We showed that each of the 15 regions we identified in two newly assembled melon genomes (subspecies melo) were successfully and accurately reconstructed as well as in a third cultivar from the agrestis subspecies. We obtained a fourfold enrichment, independently from the samples, but with some variations according to the enriched regions. In the agrestis cultivar, we further confirmed our assembly by PCR. We discussed parameters that can influence enrichment and accuracy of assemblies generated through NAS. Altogether, we demonstrated NAS as a simple and efficient approach to explore complex genomic regions. This approach finally unlocks the characterization of resistance genes for a large number of individuals, as required for breeding new cultivars responding to the agroecological transition.
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https://hal.inrae.fr/hal-04670606/file/Belinchon_et_al-bioRxiv-2023.pdf BibTex
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Characterization of a major QTL for sodium accumulation in tomato shoot
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Alexandre Héreil, M. Guillaume, R. Duboscq, Y. Carretero, E. Pelpoir, F. Bitton, C. Giraud, R. Karlova, C. Testerink, Rebecca Stevens, Mathilde Causse
article
2024
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Soil salinity is a serious concern for tomato culture, affecting both yield and quality parameters. Although some genes involved in tomato salt tolerance have been identified, their genetic diversity has been rarely studied. In the present study, we assessed salt tolerance-related traits at juvenile and adult stages in a large core collection and identified salt tolerance QTLs by genome-wide association study (GWAS). The results suggested that a major QTL is involved in leaf sodium accumulation at both physiological stages. We were able to identify the underlying candidate gene, a well-known sodium transporter, called SlHKT1.2. We showed that an eQTL for the expression of this gene colocalized with the sodium content QTL. A polymorphism putatively responsible for its variation was identified in the gene promoter. Finally, to extend the applicability of these results, we carried out the same analysis on a test-cross panel composed of the core collection crossed with a distant line. The results indicated that the identified QTL retained its functional impact even in a hybrid genetic context: this paves the way for its use in breeding programs aimed at improving salinity tolerance in tomato cultivars. Summary statement The genetic diversity of sodium accumulation in tomato shoot under salt stress is associated with an expression QTL of Sl HKT1.2. This gene, already identified as involved in tomato salinity tolerance, has been differentially selected during domestication and is of interest for the development of salinity-tolerant varieties.
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https://hal.inrae.fr/hal-04617354/file/hereil_biorxiv_2024.pdf BibTex
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Proteomic analyses of the Arabidopsis cap-binding complex define core set and TOR-dependent protein components
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Annemarie Matthes, Laurence Ouibrahim, Cécile Lecampion, Carole Caranta, Marlène Davanture, Régine Lebrun, Yohann Couté, Mathieu Baudet, Christian Meyer, Christophe Robaglia
article
2024
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The eukaryotic cap-binding complex (CBC) is a hub for regulations affecting mRNA behaviour including translation, degradation and storage. Beside the core eukaryotic translation initiation factors, other proteins, many of which are yet unknown, are thought to interact stably or transiently with the CBC depending on cell status. The prototype of these regulators is the animal eIF4E binding protein (4E-BP), a direct target of the TOR (Target of Rapamycin) kinase that competes with the cap-binding protein eIF4E, thus repressing translation. In plants, no functional homologs of 4E-BP have so far been characterized. In this work we performed several deep proteomic analyses of the Arabidopsis CBC after cap-affinity purification from wild-type plants. We also investigated the CBC in eIF4E mutant plants, Arabidopsis lines with lower TOR activity, or during infection with eIF4E-dependent potyviruses, conditions which are all affecting translation at the initiation level. These analyses allowed us to define a limited core set of CBC components, which were detected in all samples. Interestingly, we identified proteins, like AGO1 or VCS, which were always detected in conditions where either TOR or mRNA translation were reduced. Meta-analysis of these data revealed several new plant interactors of the CBC, potentially defining pathways related to mRNA stability and degradation, metabolism and viral life cycle. A search for eIF4E binding motifs identified several new potential 4E-BP relatives in plants.
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2023

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titre
Identification des bases génétiques et étude de RESIstances aux pathogènes chez la tomate résiLIENtes face aux Changements climatiquEs.
auteur
Adrien Belny, Sébastien Carrere, Jerome Gouzy, Céline Labourey, Grégori Bonnet, Laurent Grivet, Elise Albert, Tristan Mary-Huard, Richard Berthomé
article
ENVIE, Impact of Environment on plant immunity and pathogen virulence, Richard Berthomé; Mathilde Fagard; Isabelle Darboux; Thierry Langin, Nov 2023, SETE, France
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La robustesse de l’immunité végétale : une piste pour assurer la stabilité des productions agricoles face aux stress environnementaux ?
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Judith Hirsch, William Billaud, Lucie Tamisier, Félicie Lauri, Benoît Moury, Véronique Lefebvre
article
Réunion plénière 2023 du réseau ENVIE, Nov 2023, Sète, France
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Genetics underlying flavonoid variation in pepper fruit
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Yury Tikunov, Yi Wu, Sigal Popovsky-Sarid, Yelena Borovsky, Giorgio Tumino, Kobi Baruch, Pasquale Tripodi, Véronique Lefebvre, Richard G.F. Visser, Ilan Paran, Arnaud Bovy
article
XVIII International Solanaceae Genome Conference, Oct 2023, Montréal, Canada
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Pepper fruit (Capsicum spp.) is a good source of specialized nutritional metabolites such as flavonoids. The regulation of flavonoid biosynthesis has been extensively studied in Arabidopsis, tomato and petunia, but is only partially explored in pepper. In this study, a QTL on chromosome 5 of pepper was found to be highly associated with quantitative variation in flavonoids in an F2 population and later verified in NILs developed from a cross between C.annuum accessions Long Sweet and AC2212. A transcription factor highly homologous to the master regulator of the flavonoid pathway in tomato - SlMYB12 - was identified as a candidate gene underlying the effect of the locus. VIGS of CaMYB12-like in the high-flavonoid accession Long Sweet led to a significant decrease in the expression of flavonoid pathway genes and drastic decrease of flavonoid levels in silenced fruits. Overexpression of CaMYB12-like in the SlMYB12-deficient tomato y mutant led to a partial complementation of its low flavonoid phenotype. In a parallel study ripe fruit of a core collection of about 400 C.annuum accessions was developed and fruit metabolomics was carried out in the G2P-SOL project. A GWAS analysis revealed the most significant association for flavonoid variation precisely in the CaMYB12-like locus, which suggests the general key role of this gene as a master regulator of flavonoid content in C.annuum and makes it a straightforward target for classical breeding. Introgression of the CaMYB12-like allele into two cultivated varieties confirmed the potential of CaMYB12-like to breed for high flavonoid content in different pepper backgrounds.
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https://hal.inrae.fr/hal-04670151/file/Tikunov_SOL2023.pdf BibTex
titre
Evolution, history and genomics of tomato fruit size and shape
auteur
Jose Blanca, Giuseppe Aprea, Paula Ferrante, V.V. Fain, David Alonso, Lorenzo Barchi, Roland Schafleitner, R. Brandt, Mark Timothy Rabanus‐wallace, Andreas Börner, Nils Stein, Jeremy Salinier, Véronique Lefebvre, Arnaud Bovy, Hatice Filiz Boyaci, Gancho Pasev, Ivanoka Tringovska, Dani Zamir, Jaime Prohens, Joaquin Cañizares, Maria José Diez, Giovanni Giuliano
article
XVIII International Solanaceae Genome Conference, Oct 2023, Montréal, Canada
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Within the EU-funded G2P-SOL initiative, a diverse set of 15,504 accessions of tomato and its wild relatives, derived from 7 worldwide genenbaks, were genotyped, featuring >150 K variants from 5 K loci. Additionally, fruit size and shape were determined by analyzing the available historical genebank data and photographs. This dataset was utilized to uncover the evolutionary trajectories of each genomic region, and to conduct a genome-wide association study (GWAS).. Evolutionary patterns were discerned by calculating allelic frequencies across various groups, including S. pimpinellifolium (SP), wild and domesticated S. lycopersicum var. cerasiforme, Spanish vintage, Italian and American early improved materials, as well as modern improved varieties. While most loci in the genome follow a straightforward pattern—exhibiting an allele nearing fixation in cultivated varieties, distinct from the SP allele—most QTLs encompassing known fruit size and shape genes diverged from this simple pattern. Instead, the results revealed more intricate evolutionary histories, highlighting distinctions between loci selected in Spain, Italy, or within more contemporary cultivars.
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https://hal.inrae.fr/hal-04670147/file/Blanca_SOL2023.pdf BibTex
titre
Introduction to evaluation and valorization of plant genetic resources: facilitating their adoption in breeding programmes.
auteur
Véronique Lefebvre, Ignazio Verde
article
1st International Workshop and Training School on the Sustainable Management of Plant Genetic Resources, Oct 2023, Chania Crete, Greece
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Genetics underlying flavonoid variation in pepper fruit.
auteur
Yuri Tikunov, Y. Wu, Sigal Popovsky-Sarid, Yelena Borovsky, Giorgio Tumino, Baruch Ksherim, Pasquale Tripodi, Véronique Lefebvre, Richard G.F. Visser, Ilan Paran, Arnaud Bovy
article
18th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2023, Plovdiv, Bulgaria
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Pepper fruit (Capsicum spp.) is a good source of specialized nutritional metabolites such as lavonoids, which play an important role in both plant physiology and ecology as well as human nutrition. The regulation of flavonoid biosynthesis has been extensively studied in Arabidopsis, tomato and petunia, but is only partially explored in pepper. In this study, a QTL on chromosome 5 of pepper was found to be highly associated with quantitative variation in flavonoids in an F2 population and later verified in NILs developed from a cross between C. annuum accessions Long Sweet and AC2212. A transcription factor highly homologous to the master regulator of the flavonoid pathway in tomato – SlMYB12 – was identified as a candidate gene underlying the effect of the locus. VIGS of CaMYB12-like in the high-flavonoid accession cv. Long Sweet led to a significant decrease in the expression of flavonoid pathway genes and drastic decrease of flavonoid levels in silenced fruits. Overexpression of CaMYB12-like in the SlMYB12- deficient tomato y mutant led to a partial complementation of its low flavonoid phenotype. In a parallel study ripe fruit of a core collection of about 400 C. annuum accessions was developed and fruit metabolomics was carried out in the G2P-SOL project. A GWAS analysis revealed the most significant association for flavonoid variation precisely in the CaMYB12-like locus, which suggests the general key role of this gene as a master regulator of flavonoid content in C. annuum and makes it a straightforward target for classical breeding. Introgression of the CaMYB12-like allele into two cultivated varieties confirmed the potential of CaMYB12-like to breed for high flavonoid content in different pepper backgrounds.
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Conference papers
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https://hal.inrae.fr/hal-04670141/file/Tikunov_et_al_2023_Eucarpia_CapsEgg_Bulgaria.pdf BibTex
titre
Screening of a pepper collection for resistance to powdery mildew caused by Leveillula taurica, a worldwide threat
auteur
Flavie Cussonneau, Y. Awni, M. Ikhfad, Anne Massire, Christophe Gros, Carole Constant, Benoît Moury, Véronique Lefebvre
article
18. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Crops Research Institute (MVCRI); Center of Plant Systems Biology and Biotechnology (CPSBB), Sep 2023, Plovdiv, Bulgaria
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Cultivated peppers (Capsicum annuum L.) are under constant pressure from various pathogens. Among them, the ascomycete Leveillula taurica, the causal agent of powdery mildew, is responsible for severe infections worldwide, resulting in yield losses of up to 40%. The genetic leverage contributes to the current challenge aiming to reduce the use of phytosanitary products and their impact on the environment and human health. Both monogenic and polygenic resistances to L. taurica have been described in C. annuum and related species. However, either resistance remains ineffective under heavy infection, or the genetic determinants have not been sufficiently characterized to assist breeding. Therefore, we aim to establish a catalogue of resistance loci to L. taurica. For this purpose, core collections of C. annuum containing more than 600 accessions altogether were screened in different geographical regions with artificial inoculation to increase the disease pressure. The doubledhaploid line HV12 carrying resistance QTLs and a line carrying the PMR1 resistance gene were used as controls in experiments. PMR1-carrying plants showed significant symptoms, with the presence of sporulation, while HV12 plants displayed one of the highest levels of resistance. Our results suggest that the PMR1 gene used by breeding companies is not sufficient to control the disease. The data collected were used to perform a genome-wide association study (GWAS) to help identify novel genitors for powdery mildew resistance QTLs useful for pepper breeding programs.
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https://hal.inrae.fr/hal-04670120/file/Cussonneau_et_al_2023_Eucarpia_CapsEgg_Bulgaria.pdf BibTex
titre
Comparative genetic architecture of fruit traits in the three major Solanaceae fruit crops.
auteur
Giovanni Giuliano, Véronique Lefebvre, Consortium Gdeuxp-Sol
article
18th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2023, Plovdiv, Bulgaria
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Pepper, eggplant and tomato are among the most important horticultural crops worldwide. Their fruits are essential ingredients in many culinary cultures and constitute a good source of antioxidant compounds and vitamins. All three species belong to the Solanaceae family, and their diploid genomes exhibit very similar gene numbers and extensive synteny. Despite these similarities, the three species exhibit a large diversity of morphological and compositional traits, as well as different (climacteric vs non-climacteric) modes of ripening. n the G2P-SOL project, we constructed core collections of the three species, representative of their worldwide genetic diversity. These collections were genotyped/resequenced and subjected to phenotypic characterization in multiple locations, as well as to metabolic profiling of fruits. GWA analyses identified hundreds of QTLs controlling fruit morphology and composition in each species, many of which are new and exhibit high LOD scores. The genetic architecture of these traits in the three genomes will be discussed, in order to uncover common and species-specific genetic determinants controlling fruit characteristics in these species.
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A novel global Capsicum core collection delivers candidate genes for robust agronomic QTLs through a multi-environment GWAS approach
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Louis McLeod, Lorenzo Barchi, Giorgio Tumino, Pasquale Tripodi, Giovanni Giuliano, Roeland Voorrips, Ilan Paran, Véronique Lefebvre
article
18. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Crops Research Institute (MVCRI); Center of Plant Systems Biology and Biotechnology (CPSBB), Sep 2023, Plovdiv, Bulgaria
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Deciphering the genetic architecture of quantitative traits through genome-wide association mapping on core collections paves the way for breeding adapted crops. We constructed the G2P-SOL pepper world core collection, a set of 423 Capsicum spp. accessions of great genotypic and phenotypic variability, representing the diversity of more than 10,000 pepper accessions from 10 major genebanks. To tackle the modulation of complex quantitative traits by the environment and by the genotype-by-environment (GxE) interaction effects, a highly diverse subset of 350 C. annuum accessions was extensively phenotyped in a multi-location effort. Multi-environment genome-wide association studies (GWAS) were conducted for 23 agronomic traits pertaining to fruit flavor, color, size and shape, and to plant productivity, vigor and precocity. Robust QTLs and environment-specific QTLs were thus detected, and 97 well-known and new genes potentially underlying 53 of the most high-confidence and robust QTLs were highlighted. These results assert the usefulness and universality of the G2P-SOL core collection, which will be available upon request for the pepper community, alongside the genotypic and phenotypic data collected as part of the G2P-SOL project. This unique resource will be a key asset in accelerating gene discovery, developing genetic markers for marker-assisted selection, and breeding pepper varieties adapted to various climatic environments.
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https://hal.inrae.fr/hal-04670129/file/McLeod_et_al_2023_Eucarpia_CapsEgg_Bulgaria.pdf BibTex
titre
Deciphering the pepper-pathogen-environment interactions to reduce pesticide use and cope with climate change
auteur
Véronique Lefebvre
article
18th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2023, Plovdiv, Bulgaria
resume
Occurrence and severity of plant diseases are the result of tripartite interactions between the host plant, the pest or pathogen, and the environmental conditions. Management practices can affect the edges of this disease triangle for a wide variety of pests and pathogens causing significant yield losses in pepper crops (Capsicum annuum L.). Regarding the host plant, biparental progeny analyses and genome-wide association studies were conducted to identify genomic regions and candidate genes involved in plant immunity to a set of diseases as well as immunity durability traits. To uncover the factors of pathogen adaptation, the evolutionary potential of Phytophthora capsici and Potato Virus Y (PVY) is being characterized using pepper lines contrasted for alleles at resistance quantitative trait loci (QTLs). Mutational events responsible for evolution of pathogen virulence and fitness changes are being identified. Studying the influence of the host plant on the gene expression of P. capsici led to the identification of an RxLR effector that partially triggers quantitative resistance. With respect to the environment, the effects of temperature variation on immunity to P. capsici and PVY were assessed in a core collection of pepper, and QTLs affecting quantitative estimators of immunity robustness were identified. The genetic architectures of immunity, durability and robustness of immunity in various environments will be examined, to highlight key genomic regions for sustainable crop health, aimed at reducing pesticide use and coping with climate change.
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https://hal.inrae.fr/hal-04670128/file/Lefebvre_et_al_2023_Eucarpia_CapsEgg_Bulgaria.pdf BibTex
titre
Increasing multi-pest and disease resistance in stone fruit trees through genome-wide association studies
auteur
Marie Serrie, Alain Blanc, Laurent Brun, Freddy Combe, Amandine Fleury, Freddy Gavanon, Frédéric Gilles, Mathilde Le Pans, Béatrice Monnet, Véronique Signoret, Sabrina Viret, Jean-Marc Audergon, Bénédicte Quilot-Turion, Morgane Roth
article
XVI Eucarpia Symposium on Fruit Breeding and Genetics, Eucarpia; International Society for Horticultural Sciences (ISHS); Julius Kühn-Institut (JKI), Sep 2023, Dresden (GERMANY), Germany
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Stone fruit trees must face a multi-disease and pest challenge context over their entire lifetime, incurring for significant damages and economic losses. To date we actually lack varieties that combine a low level of susceptibility against several of these pests and diseases and more information is still needed to decipher the genetic architecture underlying these resistances for stone fruit trees. Our objective is thus to identify the genetic components of multi-disease resistance of two major stone fruit tree species: apricot and peach. To do so, two core collections composed by 150 apricot and 206 peach replicated accessions are maintained under low phytosanitary conditions respectively in two and three contrasted environments in South-East of France. These accessions have been densely genotyped with the Illumina HiSeq 2000 NGS technique for apricot and the IRSC 16K SNP array for peach. We monitored the multiple symptoms of respectively eight and five pests and diseases over the years since 2020 for apricot and 2021 for peach. Thanks to this integrative phenotyping on several sites and years, we were able to study the interactions between pests and diseases and we successfully identified accessions with lower susceptibilities. After deciphering the effects of genotype, environment and their interactions, we performed genome-wide association studies (GWAS) which allowed us to identify genetic markers linked to resistance to these diverse sources of pathogens. These analyses were conducted jointly on diseases affecting both apricot and peach, such as rust, in order to compare the genetic architecture of disease resistance for these two closely related species. Next steps will include multi-trait GWAS to exploit genetic correlations between traits. This work already paves the way for future marker assisted selection for stone fruit trees in order to make multi-disease resistance a concrete goal for breeders.
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Fine-tuning genomic prediction in peach using additive and non-additive genetic effects for phenology and disease tolerance
auteur
Morgane Roth, Marie Serrie, Octave Cabel, David Ray, Jean-Marc Audergon, Bénédicte Quilot-Turion
article
Eucarpia Fruit Genetics and Breeding, Julius Kühn Institute, Sep 2023, Dresden, Germany
resume
Genomic predictions rely on modelling genome-wide marker effects and are especially powerful to predict complex traits. Like for other perennial fruit crops, the implementation of genomic selection could have a big impact on breeding programs by shortening the breeding schemes and/or gaining in selection intensity and reliability. Despite vast available genomic resources in public institutions, both in terms of plant material and genomic information, only few studies have assessed prediction accuracy in peach. In this study, we explore the potential of genomic selection by calculating genomic estimated breeding values for traits with contrasted genetic architecture. As it is known that non-additive effects and directional dominance can strongly affect trait variation, and because these effects can be easily fixed in clonally propagated crops, we also proposed a model comparison from the simplest, purely additive model, to a more complete model accounting for inbreeding, dominance and epistatic effects. Our training set is a core-collection of 192 accessions planted in three sites in different climatic regions of South-East France, and phenotyped for green tip emergence, flowering date (three years of observation), and for leaf curl symptoms observed under zero pesticide (one year of observation). Using linear mixed models, we will present the impact of inbreeding and non-additive effects on trait mean and variance in peach. Genomic prediction will be tested with classical rrBLUP as well as GBLUP approaches using a total of six different models accounting for different combinations of genetic variance and inbreeding parameters. Across-site predictions for phenological traits over years and environments will also be tested. In a translational perspective, it is planned to use the same approach on apricot, a crop closely related to peach, to compare results in the light of crop biology and trait architecture.
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Resistance to viruses based on eIF4E: from natural variation to edited genes
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Jean-Luc Gallois
article
12th International Congress of Plant pathology ICPP2023, The International Society for Plant Pathology; The French Phytopathological Society, Aug 2023, Lyon, France
resume
eIF4E translation initiation factors have emerged as major susceptibility factors for RNA viruses. Natural eIF4E-based resistance alleles are found in many species and are mostly variants that maintain the translation function of the protein. eIF4E genes represent therefore targets for engineering viral resistance, and gene-editing technologies can be used to make up for the lack of natural resistance alleles in some crops. However, redundancy among eIF4E genes can restrict the efficient use of knockout alleles in breeding. Using Arabidopsis, we previously showed how gene-editing technologies can be used to design de novo functional alleles, using knowledge about the natural evolution of eIF4E genes in different species, to drive resistance to viruses without affecting plant physiology. Here, we will also present how this knowledge can be applied to a crop, tomato, using CRISPR-Cas9 base editing. We will show that there is a trade-off to find between resistance and functionality, and discuss these results in the light of resistance durability.
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In search of an attractive kairomone for biological control by mass trapping of Eurytoma amygdali
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Anjélica Leconte, Eugénie Coutagne, Henri Duval, Philippe Lucas, Michael Staudt
article
38th Annual Meeting of the International Society of Chemical Ecology, R. Borges, Jul 2023, Bangalore, India. pp.69
resume
Eurytoma amygdali End., the almond wasp, is an important pest in organic almond orchards potentially causing high crop failure in the southeast of France and in several countries in the Middle East. Currently there is no biological control method against E. amygdali and only one effective pesticide is authorized in France. In order to develop a biocontrol strategy against wasp infestation, we have identified and quantified by GC-MS the volatile organic compounds (VOCs) of the almond tree (Prunus dulcis) in spring, when females are attracted by almond trees to lay eggs in almonds in development, under environmentally-controlled conditions. Emissions of VOCs from almond trees were low and mainly composed of sesquiterpenes. Other VOC classes have been identified as monoterpenes, aldehydes, alcohols, esters and anisole. To find the volatiles most likely to attract E. amygdali during the day we have monitored VOC emissions according to temperature and light gradients by PTR-ToF-MS and GC-MS, with five varieties of P. dulcis during flowering and fruit and leaf ripening. Emissions increased significantly with temperature but not with light. Depending on the almond variety and the phenological stage, the composition and the quantities of volatiles emitted were different. Thirty-eight compounds were then tested by electroantennography (EAG) to observe their detection by the female antennae and identify the most active VOCs. The compounds best detected by females are not those most emitted by the almond tree. Only five compounds did not elicit significant EAG responses when compared to control stimuli and no compounds from the panel were much more active than the rest of EAG-active compounds. We are currently testing the attractiveness on wasps of mixtures of the VOCs most emitted by the almond tree and the most active in EAG in order to develop a kairomone trap, a means of biocontrol by massive trapping against the almond wasp.
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An iterative gene-editing strategy broadens eIF4E1 genetic diversity in Solanum lycopersicum and generates resistance to multiple potyvirus isolates
auteur
Kyoka Kuroiwa, Benoit Danilo, Florian Veillet, Fabien Nogué, Marianne Mazier, Jean-Luc Gallois
article
Plant Biology europe 2023, the French Society of Plant Biology, Jul 2023, Marseille, France
resume
Resistance to potyviruses in plants has been largely provided by the selection of natural variant alleles of eukaryotic translation initiation factors (eIF) 4E in many crops. However, the sources of such variability for breeding can be limited for certain crop species, while new virus isolates continue to emerge. Different methods of mutagenesis have been applied to inactivate the eIF4E genes to generate virus resistance, but with limited success due to the physiological importance of translation factors and their redundancy. Here, we employed genome editing approaches at the base level to induce non-synonymous mutations in the eIF4E1 gene and create genetic diversity in cherry tomato (Solanum lycopersicum var. cerasiforme). We sequentially edited the genomic sequences coding for two regions of eIF4E1 protein, located around the cap-binding pocket and known to be important for susceptibility to potyviruses. We show that the editing of only one of the two regions, by gene knock-in and base editing, respectively, is not sufficient to provide resistance. However, combining amino acid mutations in both regions resulted in resistance to multiple potyviruses without affecting the functionality in translation initiation. Meanwhile, we report that extensive base editing in exonic region can alter RNA splicing pattern, resulting in gene knockout. Altogether our work demonstrates that precision editing allows to design plant factors based on the knowledge on evolutionarily selected alleles and enlarge the gene pool to potentially provide advantageous phenotypes such as pathogen resistance.
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Les variétés d’hier et de demain à la rescousse pour construire un verger zéro pesticide !
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Morgane Roth, Marie Serrie
article
Pint of Science, May 2023, Avignon, France
resume
Nous demandons aujourd’hui aux arbres fruitiers de se débrouiller sans pesticide, mais en sont-ils capables ? Venez découvrir pourquoi et comment mobiliser la diversité génétique pour créer des nouvelles variétés qui répondent à ce challenge. A INRAE nous cherchons à définir ce qu’est un arbre fruitier résilient qui saurait faire face aux défis d’un verger zéro-phyto. Les connaissances en génétique peuvent nous permettre d’atteindre plus rapidement cette cible, notamment en aidant les sélectionneurs à trouver les meilleures combinaisons de parents pour créer les futures générations d'arbres.
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Detection of volatile emissions from almond trees and electroantennographic response of the almond wasp (Eurytoma amygdali) to these volatiles
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Henri Duval, Anjélica Leconte, Adrien Le Navenant, Philippe Lucas, Eugénie Coutagne, Michael Staudt
article
VIII International Symposium on Almonds and Pistachios, University California, May 2023, Davis, United States
resume
Eurytoma amygdali Enderlein, the almond wasp, is an important pest in almond orchards in southeastern France. In order to develop a biocontrol strategy against wasp infestations, we studied the emission of volatiles emitted by almond trees and their detection by adult almond wasps. Our approach consists in identifying and quantifying volatiles released by almond trees at different stages in headspace by GC-MS (Gas Chromatography-Mass Spectrometry) and PTR-ToF-MS (Proton Transfer Reaction - Time of Flight - Mass Spectrometry). These volatile organic compounds (VOCs) are then tested by electroantennography (EAG) and EAG coupled to GC (GC-EAD) to measure their detection by the female antennae. The detected VOCs will be used to test simple mixtures at different ratios on the behavior of wasps in order to develop a kairomone trap.
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Diversity analysis of the INRAE and IRTA collection from the Axiom 60K SNP almond array
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Henri Duval, Felipe Perez de Los Cobos, Eva Coindre, Ignasi Batlle, Iban Eduardo Muñoz, Bénédicte Quilot-Turion, Pere Arús
article
VIII International Symposium on Almonds and Pistachios, University of California, May 2023, Davis, United States
resume
Following the works of the “almond genome” consortium, and the publication of the almond genome reference “Texas”, we developed a 60K SNPs axiom almond array as tools for almond breeders and genetic studies. From a pool of 80 resequenced varieties, a set of 70k SNPs markers was filtered for the development of the array and the Affymetryx score retained 60581 SNPs markers. These SNP were well distributed along the eight chromosomes. The mean and the median for the distance between following SNPs were 2891 bp and 901 bp respectively and there were two gaps with more than 300 000 bp on chromosomes 1 and 2. With this array, we genotyped 219 almond accessions available in the two collections of INRAE in Avignon and IRTA in Reus. We excluded from our statistical analysis wild and hybrid accessions and SNPs with more than 10% of missing data. At the end of the process, we had an initial matrix with 152 individuals and 54K SNPs. Analysis with the SNMF R package showed that two models with five or seven ancestral groups were most probable. The model with five groups contained 21, 14, 19, 14, 12 varieties, and the first three groups included mainly Spanish, Italian and Chinese varieties respectively, while the two other groups and the admixture group contained different origins. The Kinship analysis highlighted the relationships between the varieties and we observed that the identified clusters corresponded well with to origin of the countries. This was particularly the case for the Italian, Chinese, Californian and Spanish varieties. The French varieties were more scattered. Our analysis showed that data provided by the axiom almond array were of good quality. The future steps of our analysis will be the GWAS analysis with some phenological and nut quality traits.
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Defining and characterizing the phenotypic components of disease resilience in stone fruit trees
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Marie Serrie
article
Young Researchers' Days - BAP Department - 2023, INRAE, Apr 2023, Clermont-Ferrand, France
resume
Fruit tree orchards are faced to multiple pests and diseases which are responsible for significant damages and pesticidesstill remain the most effective means of control. In a context of urgent demand for the reduction of phytosanitary products, alternative and durable solutions must be found. To address these challenges, the selection of resilient trees, i.e. trees that are minimally affected by perturbations or which rapidly return to the state pertained before exposure to these disturbances, seems to be a promising but still misunderstood and under-exploited solution. Thus, a first part of my PhD project aims to define and characterize the phenotypic components of resilience in fruit trees. To do so, two core collections of two stone fruit tree species (apricot and peach) with a high genetic diversity have been deployed and are maintained under low phytosanitary conditions in five contrasted environments. By evaluating the symptoms of the diverse pests and diseases over the years, we might be able to decipher the different strategies and phenotypic manifestations of resilience.
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Breeding for resistance/tolerance in fruit crops: New trends
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Bénédicte Quilot-Turion
article
Plant Health Workshop: Advances in plant disease and pest management: a holistic approach, IRTA, Feb 2023, Cabrils, Spain
resume
Focusing on multi-character integration and tree resilience : preserve, screen and study large Prunus genetic resources including wild and relatives and use modern tools and approaches to progress towards developing multi-trait selection for low input production systems (optimization of schemes and methods of selection, genomic selection) and design prototypes to 'protect' the resistances and to respond to multiple stresses.
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Lipid droplets : New actors of the plant virus infection
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Lena Jambou, Luc Sofer, Nathalie Marissal-Arvy, Vincent Simon, Denis Coulon, Claire Bréhélin, Sylvie German-Retana, Jean-Luc Gallois
article
19èmes rencontres de Virologie Végétale, Jan 2023, Aussois, France
resume
Lipid droplets (LDs) are organelles composed of a phospholipid monolayer enclosing neutral lipids, involved in energy production, membrane biogenesis and stress signaling. Recently, LDs have also been shown to be required by some animal (+) RNA viruses for their replication. Like animal viruses, plant (+) RNA viruses have to hijack host proteins, intracellular membranes, and lipids to create an optimized lipid/membrane microenvironment for their efficient viral replication compartment (VRC) assembly. Yet the possible involvement of LDs in plant virus infection remains totally unexplored. Here, our confocal studies revealed that infection by turnip mosaic virus (TuMV, potyvirus) does induce a statistically significant increase of the number of LDs compared to mock-inoculated leaves, both in Arabidopsis and Nicotiana benthamiana. We also evidenced a significant accumulation of neutral lipids in infected leaves, thus confirming that TuMV-infection induces LD biogenesis. In addition, Transmission Electron Microscopy data revealed that the newly formed LDs are recruited to the TuMV-induced VRCs. Interestingly, potato virus A (PVA), another potyvirus species, induces a comparable LD biogenesis. By comparing the mRNA translatomes of TuMV- and mock- infected A. thaliana leaves, we detected in TuMV infected samples an increased translation of the main structural proteins of the leaf LDs, namely the three LDAPs (Lipid Droplet Associated Proteins) and the caleosin CLO3, which is in perfect agreement with the increase in the number of LDs induced by TuMV. Preliminary ELISA assays suggest that an Arabidopsis ko mutant of SEIPIN (a protein involved in the LD budding from the ER) seems less susceptible to TuMV, while LDAP1 overexpressor line (that shows a higher number of LDs than the wild type) displays higher susceptibility. Altogether, our results suggest for the first time, that LD biogenesis plays a pro-viral role in a plant virus infection.
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A plant-specific homolog of DP1/Yop1 family proteins plays a proviral role in potyvirus infection
auteur
Mingshuo Xue, Luc Sofer, Vincent Simon, Roxane Lion, Nathalie Marissal-Arvy, Mamoudou Diop, Jean-Luc J.-L. Gallois, Jens Tilsner, Sylvie German-Retana
article
19èmes Rencontres de Virologie Végétale, Aussois, France, Jan 2023, Aussois, France
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The Potyvirus genus is one of the largest genera of plant RNA viruses responsible for serious diseases in crops worldwide. As potyviruses developed strategies to hijack the host secretory pathway and plasmodesmata (PD) for their transport, the goal of this study was to identify membrane and/or PD-proteins that interact with the 6K2 protein, a potyviral protein involved in replication and cell-to-cell movement of turnip mosaic virus (TuMV). Using Split-ubiquitin membrane Y2H assays we screened an Arabidopsis cDNA library for interactors of the TuMV-6K2. We isolated AtHVA22a (Hordeum vulgare abscisic acid responsive gene 22) that belongs to a multigenic family of transmembrane proteins, homologous to DP1/Yop1 family proteins in yeast and interactors of reticulons. HVA22 DP1/Yop1 family genes are widely distributed in eukaryotes, but the role of HVA22 proteins in plants are not well-known, except the role in blast disease resistance recently described in rice [1]. Interestingly, proteomics analysis of PD fractions purified from Arabidopsis suspension cells showed that AtHVA22a is highly enriched in plasmodesmata proteome, making it a good candidate for the virus cell-to-cell movement [2]. We further confirmed the interaction between 6K2 and AtHVA22a in yeast, as well as in planta by using bimolecular fluorescence complementation (BiFC). Furthermore, we showed that the 6K2/AtHVA22a interaction occurs at the level of the viral replication complexes (VRC) during TuMV infection and partially at PD. The partial relocalization of AtHVA22a at the VRC during TuMV infection seems to be specific to potyvirus infection as it is not induced on potexvirus infection. Finally, we showed that the propagation of TuMV in arabidopsis leaves is increased when AtHVA22a is overexpressed in planta but slowed down upon inactivation of AtHVA22a by CRISPR-Cas9. Altogether, our results indicate that AtHVA22a plays an agonistic effect on TuMV propagation. [1] doi: 10.1016/j.celrep.2022.110941. [2] doi: 10.15252/embr.201847182.
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titre
An iterative gene-editing strategy broadens eIF4E1 genetic diversity in Solanum lycopersicum and generates resistance to multiple potyvirus isolates
auteur
Kyoka Kuroiwa, Benoit Danilo, Florian Veillet, Fabien Nogué, Marianne Mazier, Jean-Luc Gallois
article
19ème Rencontre de Virologie Végétale, Jan 2023, Aussois, France
resume
Resistance to potyviruses in plants has been largely provided by the selection of natural variant alleles of eukaryotic translation initiation factors (eIF) 4E in many crops. However, the sources of such variability for breeding can be limited for certain crop species, while new virus isolates continue to emerge. Different methods of mutagenesis have been applied to inactivate the eIF4E genes to generate virus resistance, but with limited success due to the physiological importance of translation factors and their redundancy. Here, we employed genome editing approaches at the base level to induce non-synonymous mutations in the eIF4E1 gene and create genetic diversity in cherry tomato (Solanum lycopersicum var. cerasiforme). We sequentially edited the genomic sequences coding for two regions of eIF4E1 protein, located around the cap-binding pocket and known to be important for susceptibility to potyviruses. We show that the editing of only one of the two regions, by gene knock-in and base editing, respectively, is not sufficient to provide resistance. However, combining amino acid mutations in both regions resulted in resistance to multiple potyviruses without affecting the functionality in translation initiation. Meanwhile, we report that extensive base editing in exonic region can alter RNA splicing pattern, resulting in gene knockout. Altogether our work demonstrates that precision editing allows to design plant factors based on the knowledge on evolutionarily selected alleles and enlarge the gene pool to potentially provide advantageous phenotypes such as pathogen resistance.
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Trying to develop genetic resistances to potyvirus based on S gene : Analysis of redundancy among chloroplastic PhosphoGlycerate Kinase in Arabidopsis
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Jean-Luc Gallois, Lucas Laflaquière, Nathalie Giovinazzo, Mamoudou Diop
article
19ème Rencontre de Virologie Végétale, Jan 2023, Aussois, France
resume
Plant genes encoding susceptibility factors are good targets to develop genetic resistances to viruses. Although resistances to potyviruses mainly rely on the translation initiation factors such as eIF4E, it is important to characterize new host susceptibility factors. Here, using the Arabidopsis thaliana-potyvirus pathosystem, we assessed an approach to develop genetic resistance to watermelon mosaic virus (WMV) based on cPGKs, which are metabolic enzymes that acts as a susceptibility factor to several plant viruses. We show that inactivation of cPGK2, that has been characterized as a natural resistance allele in the Arabidopsis accession Cvi, is not sufficient to trigger resistance. We reasoned that this could be caused by the existence of a gene closely homolog to cPGK2 - cPGK1- that could also be targeted by the virus. Interestingly, cPGK1 accumulates predominantly over cPGK2 in leaf cells, suggesting that knocking out cPGK2 should not be sufficient to lower the level of PGK susceptibility factors. We therefore analysed the potential mechanism that could explain cPGK2-based natural resistance in Cvi. The polymorphism in the cPGK2 allele of Cvi does not seem to alter its chloroplast addressing, which rules out a disruption of chloroplast targeting as a direct explanation for Cvi resistance. Interestingly, significantly lower levels of cPGK1 mRNA accumulates in the Cvi accession: the natural resistance in Cvi could simultaneously result from a mutated cPGK2 and a lower abundance of cPGK1. Altogether, these results highlight that developing PGK-based resistances in plants could face difficulties already encountered in the development of eIF4E-based resistance, such as the role of genetic redundancy and the need to maintain a balance between plant development and resistance engineering. Our results suggest that Arabidopsis could be a good model to understand the complexity of cPGK-based susceptibility/resistance, with the aim of translating this mechanism into effective resistances into crops.
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Multiple Eggplant Domestication/Adaptation Events Revealed by Genetic Analysis of Around 3,500 Worldwide Accessions
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Lorenzo Barchi, Emmanuel Omondi, Giuseppe Aprea, Mark Timothy Rabanus‐wallace, Laura Toppino, David Alonso, Ezio Portis, Sergio Lanteri, Luciana Gaccione, Maarten van Zonneveld, Roland Schafleitner, Paola Ferrante, Andreas Boerner, Nils Stein, Maria José Díez, Véronique Lefebvre, Jérémy Salinier, Hatice Filiz Boyaci, Richard Finkers, Matthijs Brouwer, Arnaud Bovy, Giuseppe Leonardo Rotino, Jaime Prohens, Giovanni Giuliano
article
Plant & Animal Genome XXX Conference, Jan 2023, San Diego (CA), United States
resume
Eggplant (Solanum melongena L., 2n=2x=24), also known as brinjal or aubergine, is a member of the Solanaceae family and the third most widely grown Solanaceous vegetable after potato and tomato, with a global production of 57 Mt in 2020 (FAOSTAT). In the frame of the EU G2P-SOL project (https://www.g2p-sol.eu/), about 5,900 accessions of eggplant and wild relatives belonging to its primary, secondary and tertiary gene pool were inventoried. A custom set of 5K Single Primer Enrichment Technology (SPET) probes was applied for genotyping around 3,500 accessions originating from 105 countries in five continents and maintained in seven genebanks. After SNPs calling, a whole of 88 misclassified and 591 duplicated accessions were identified. Various hypotheses have been formulated on eggplant domestication, including its origin from a single or from two domestication centers. Our data suggest that domestication occurred independently in South-East Asia and in the Indian subcontinent, with limited genetic exchange between the two regions, and also that migration and consequent admixture of genotypes from different regions has been much more limited than in tomato and pepper, the other two widely cultivated berry-producing Solanaceous crops. Genome-wide association analysis of genetic and phenotypic data scored by genebanks during seed regeneration, in combination with pedo-climatic data from the Indian diversity center, highlighted the role of both anthropogenic and environmental selection in shaping the extraordinary phenotypic and genetic diversity of this crop.
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Exploration of High-Throughput Data for Heat Tolerance Selection in Capsicum annuum
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Nathan Fumia, Michael Kantar, Ya‐ping Lin, Roland Schaftleitner, Véronique Lefebvre, Ilan Paran, Andreas Boerner, Maria José Díez, Jaime Prohens, Arnaud Bovy, Hatice Filiz Boyaci, Gancho Pasev, Pasquale Tripodi, Lorenzo Barchi, Giovanni Giuliano, Derek Barchenger
article
Plant and Animal Genome XXX Conference, Jan 2023, San diego, CA, United States
resume
Recently there has been a substantial increase in high-throughput technologies which generate highly complex large datasets for use in the sciences. Plant breeding and genetics is one field that has benefited from this data explosion where many public and private institutions now implement genomic and phenomic data to predict performance thus informing germplasm selection. However, the multitude of methodologies and data generate a situation of strategic uncertainty. We set out to compare different methods of genomic and phenomic selection in the Capsicum annuum core collection, developed through the G2P-Sol project, producing a combination of unique and similar selected genotypes for heat tolerance. Combined, the methods tested identified a total of 33 genotypes that show tremendous promise for use as parents in heat tolerance breeding; with 13 of these being present in more than one selection method. Combining classical and multispectral phenotyping methods produced better selection results and when separated, comparable results are obtained. Our weighted rank sum index identified 10 genotypes across environments that show heat tolerance, 8 of which are also selected within heat environments. This suggests that different breeding programs can reach similar results despite having different logistical constraints. Therefore, our case study into selection of pepper germplasm with phenomic and genomic data exhibits the potential to compensate for dearth of germplasm knowledge with high-throughput data as well as the converse, to compensate for logistical or financial constraint to new technologies with breeder knowledge.
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Poster communications

titre
Phylogenetic drivers involved in the diversity of plant volatile profiles shape antixenosis resistance of tomato against Tuta absoluta
auteur
Komla Exonam Amegan, Yasmine Fourati, Romain Larbat, Bernard Caromel, Anne Violette Lavoir, Christophe Robin, Alan Kergunteuil
article
XII European Congress of Entomology (ECE 2023), Oct 2023, Heraklion, Greece.
resume
Volatile organic compounds (VOCs) are of first importance in plant defenses since they alter host-plant location by herbivore insects. The worldwide distribution of tomato offers to explore the ecological diversity of defensive VOCs produced by plants supporting a large range of local adaptations. In this study, first, we analyzed the diversity of VOC profiles released by 29 tomato accessions with different natural history and including domesticated and wild relatives. Second, behavioral assays were performed on 10 accessions characterized by strong differences in VOC emissions to elucidate the mechanisms underlying the relationships between the diversity of volatile profiles and antixenosis resistance against a pest of tomato, Tuta absoluta. Among the 77 VOCs detected, a wide diversity of volatile profiles of tomato is reported at both inter- and intra-specific levels. A phylogenetic tree was constructed to study the role of phylogenetic drivers in the distribution of VOC diversity released by tomato. The volatile profile richness and sesquiterpenoid emissions are relatively high in ancestral branches and decreased rapidly with evolution according to an early-burst model. As compared to wild relatives, this study confirmed that domesticated S. lycopersicum with poor volatile profiles and low emissions of sesquiterpenoid is attractive to T. absoluta. Four repellent accessions belonging to S. pennellii, S. habrochaites and S. cheesmaniae were identified. Results stress the importance of both inter- and intra-specific diversity of VOC profiles in antixenosis against T. absoluta. β-myrcene, methyl salicylate and sesquiterpenoids such as (3E,7E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene or α-curcumene could serve as a repellent, whereas δ-elemene and 2-phenylacetaldhyde may help T. absoluta to locate host plants.
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Unraveling the quantitative pepper - Phytophthora capsici interplay: global assessment of isolate aggressiveness
auteur
Guillermo Merino Martin, Anne Massire, Alexandre Bachellez, Suzan H. E. J. Gabriëls, Vivianne G a A Vleeshouwers, Yuling Bai, Véronique Lefebvre
article
18th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2023, Plovdiv, Bulgaria
resume
Pepper is one of the most economically important crops worldwide, but its production is often challenged by plant pathogens such as Phytophthora capsici. This oomycete can cause devastating losses, and its high level of global genetic diversity poses a significant challenge for breeding programs aimed at developing resistant pepper varieties. It is acknowledged that resistance to P. capsici mostly follows a nested pattern. However, to better understand the interactions between pepper and P. capsici, we conducted a study to investigate how different P. capsici isolates from various regions of the world interact with different pepper accessions. We inoculated 11 P. capsici isolates from France, Mexico, Korea, China, the USA, and the Asian Pacific on 9 pepper accessions (Capsicum annuum) with varying degrees of resistance to the pathogen. We then assessed the symptoms of the disease. Our results revealed two key findings. Firstly, we observed a quantitative aspect to the plant-pathogen interaction, with some pepper accessions being more resistant to certain P. capsici isolate than others.Secondly, we observed a diverse range of symptom intensities, demonstrating that some P. capsici isolates have high aggressiveness and can infect a broad range of pepper accessions, while others have low aggressiveness and infect fewer accessions. These findings provide important insights on the P. capsici infectivity patterns and have significant implications for breeding programs aimed at developing resistant pepper varieties. By characterizing the isolates and accessions involved in these interactions, we can begin to better understand the complex dynamics of plant-pathogen interactions and develop more targeted breeding strategies to combat this evastating pathogen. In summary, our study highlights the challenges posed by P. capsici to pepper production and provides valuable insights into the complex interactions between this pathogen and its host.
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An iterative gene-editing strategy broadens eIF4E1 genetic diversity in Solanum lycopersicum and generates resistance to multiple potyvirus isolates
auteur
Kyoka Kuroiwa, Benoit Danilo, Florian Veillet, Fabien Nogué, Marianne Mazier, Jean-Luc Gallois
article
12th International congress of Plant Pathology, Aug 2023, Lyon, France
resume
Resistance to potyviruses have been largely provided by the selection of natural variant alleles of eukaryotic translation initiation factors (eIF) 4E in many crops. However, the sources of such variability are limited for certain species. Different methods of mutagenesis have been applied on the eIF4E genes to generate virus resistance, but with limited success due to their physiological importance and redundancy. Here, we sequentially edited in cherry tomato the genomic sequences coding for two regions of eIF4E1, known to be important for susceptibility to potyviruses. We show that editing of one region is not sufficient but combining amino acid mutations in both regions result in resistance to multiple potyviruses without imparing translation initiation. Our work demonstrates that precision editing allows to design plant factors based on the evolutionarily selected alleles and enlarge the gene pool to potentially provide advantageous phenotypes such as pathogen resistance.
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The in-frame-deletion allele-encoded eIF4E protein confers resistance to cucumber mosaic virus by inhibiting 2B in tomato
auteur
Ghos Sozib, Ayaka Kawabuko, Hiroki Atarashi, Miyuki Suto, Kyoka Kuroiwa, Jean-Luc Gallois, Kenji Nakahara
article
12th International Congress of Plant Pathology, Aug 2023, Lyon, France
resume
Both the host organisms and the infecting viruses rely on the eukaryotic translation initiation factor 4E (eIF4E) for gene expression. Plants utilize the genes from the eIF4E family to develop antiviral resistance. The mechanisms of resistance previously studied are attributed to the lack of proper interactions of the resistant eIF4E protein with viral proteins, hindering the ability of targeted viruses to infect. Our research has demonstrated an alternative mode of eIF4E- mediated resistance. We used CRISPR/Cas9 to create tomato eIF4E1 alleles, including one with an in-frame 9-nucleotide deletion (9DEL) within the eIF4E1 coding sequence. Unlike a 1- nucleotide insertion knockout allele, we showed that the 9DEL deletion was associated with a significant reduction in susceptibility to cucumber mosaic virus (CMV). Here, we present new data shedding light on the molecular mechanism behind the 9DEL-mediated resistance to CMV. The 9DEL-encoded mutant eIF4E1 was found to bind to the CMV-encoded protein 2b through a strong affinity to the C-terminal of 2b. We have previously reported that 2b is a target of autophagy, a primary proteolytic pathway. Our results suggest that the 9DEL appears to enhance the 2b degradation. Consistently, the RNA silencing suppressor activity of 2b was weakened when co-expressed with 9DEL. Since 2b was essential for systemic CMV infection in tomato, we propose that the 9DEL mutation confers resistance to CMV on tomatoes by binding to and inhibiting 2b.
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A PLANT-SPECIFIC HOMOLOG OF DP1/YOP1 FAMILY PROTEINS PLAYS A PROVIRAL ROLE IN POTYVIRUS INFECTION
auteur
Sylvie German-Retana, Luc Sofer, Vincent Simon, Jean‐luc Gallois, Amandine Bordat, Nathalie Arvy, Mamoudou Diop, Jens Tilsner
article
12th INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, Aug 2023, Lyon, France
resume
The Potyvirus genus is one of the largest genera of plant RNA viruses responsible for serious diseases in crops worldwide. As potyviruses hijack the host secretory pathway and plasmodesmata (PD) for their transport, the goal of this study was to identify membrane and/or PD-proteins that interact with the 6K2 protein, a potyviral protein involved in replication and cell-to-cell movement of turnip mosaic virus (TuMV). Using Split-ubiquitin membrane Y2H assays we screened an Arabidopsis cDNA library for interactors of TuMV-6K2. We isolated AtHVA22a (Hordeum vulgare abscisic acid responsive gene 22) that belongs to a multigenic family of proteins homologous to DP1/Yop1 family proteins in yeast and interactors of reticulons. The role of HVA22 proteins in plants are not well-known, except the role in blast disease resistance in rice. Interestingly, proteomics analysis of PD fractions showed that AtHVA22a is highly enriched in Arabidopsis plasmodesmata proteome. We confirmed the interaction between 6K2 and AtHVA22a in yeast, as well as in planta by using bimolecular fluorescence complementation (BiFC) and showed that the interaction occurs at the level of the viral replication complexes (VRC) during TuMV infection. Finally, we showed that the propagation of TuMV in plants is increased when AtHVA22a is overexpressed but slowed down upon mutation of AtHVA22a by CRISPR-Cas9. Altogether, our results indicate that AtHVA22a plays an agonistic effect on TuMV propagation.
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https://hal.inrae.fr/hal-04648035/file/Xue-et-al-ICPP2023-Poster.pdf BibTex
titre
Systematic parent-specific expression perturbations in hybrid endosperms may explain how effective ploidy differences cause hybrid seed failure
auteur
Morgane Roth, Ana Florez-Rueda, Thomas Städler
article
Plant Biology Europe, Jul 2023, Marseille, France
resume
Hybrid seed inviability (HSI) is a common post-zygotic reproductive barrier in angiosperms caused by endosperm mis-development. Recent studies suggest that “effective ploidy” divergence driven by parental conflict may be responsible for HSI, yet these phenomena remain poorly characterized at the molecular level. We analyzed endosperm transcriptomes in 12 reciprocal crosses within and among three diploid wild tomato lineages and tested 5,015 endosperm-expressed informative genes common to all hybrid crosses for Differential Parental Expression (DPE between hybrid and their corresponding intraspecific endosperms). Strikingly, allelic expression is systematically more perturbed in the parent with lower effective ploidy. We illustrate and discuss the complex interplay between parental expression proportions, total and parent-specific expression in abortive endosperms. Overall, our analyses yield candidate genes/pathways that may underpin the unknown epigenomic imbalances underlying HSI.
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Resistance to viruses based on eIF4E: from natural variation to edited genes
auteur
Jean-Luc Gallois, Kyoka Kuroiwa, Delyan Zafirov, Caroline Lebaron, Nathalie Giovinazzo, Christophe Robaglia, Fabien Nogué, Marianne Mazier
article
Plant Biology Europe 2023, Jul 2023, Marseille, France
resume
eIF4E translation initiation factors have emerged as major susceptibility factors for RNA viruses. Natural eIF4E-based resistance alleles are found in many species and are mostly variants that maintain the translation function of the protein. eIF4E genes represent therefore targets for engineering viral resistance, and gene-editing technologies can be used to make up for the lack of natural resistance alleles in some crops. However, redundancy among eIF4E genes can restrict the efficient use of knockout alleles in breeding. Using Arabidopsis and Tomato, we show how gene-editing technologies can be used to design de novo functional alleles, using knowledge about the natural evolution of eIF4E genes in different species, to drive resistance to viruses without affecting plant physiology. We will show that there is a trade-off to find between resistance and functionality, and discuss these results in the light of resistance durability.
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Deciphering plant resilience mechanisms to face the multiple disease challenge in fruit trees
auteur
Marie Serrie, Fabienne Ribeyre, Laurent Brun, Jean-Marc Audergon, Bénédicte Quilot-Turion, Morgane Roth
article
Plant Biology Europe 2023, Jul 2023, Marseille, France
resume
As perennial plants, fruit trees must cope, individually, with the fluctuating threat of multiple pathogens over the years. In this long-lasting battle for plant immunity, disease resilience is emerging as a key mechanism for tree survival and fitness. More fundamental research is required to improve our understanding of disease resilience mechanisms, which in turn could be particularly relevant for a more sustainable fruit production. As this approach is novel for that field, we propose i) a clear definition of resilience for fruit producing trees, ii) a methodology for studying its phenotypic components which requires repeated measures of “resilience biomarkers”, iii) to decipher the genetic architecture of resilience components, and iv) an innovative strategy based on high-throughput phenotyping and genomics for identifying resilient genotypes. All in all, disease resilience appears as a meaningful breeding perspective in a context of unprecedented plant protection restrictions.
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https://hal.inrae.fr/hal-04155954/file/Poster_PBE_Serrie.M.pdf BibTex
titre
The French project LEVEAB: Remove the barriers to growing almonds in organic farming
auteur
Henri Duval, Jean-Michel Montagnon, Philippe Lucas, Jean-Philippe Rouvier, Jimmy Planche, Margaux Allix, Myriam Berud, François Warlop, William Chambeyron, Carole Calcet, Benoît Chauvin-Buthaud, Florence Fraisse, Cédric Portal
article
VIII International Symposium on Almonds and Pistachios:, May 2023, Davis, CA, United States
resume
Despite strong demand, organic almond production in France is limited by pest pressure. The lack of sustainable solutions to control pests and diseases (Eurytoma, Fusicoccum, etc.) is holding back the development of the organic sector and limiting the scope for agroecological progress in conventional production. In 2021, we started the LEVEAB project, whose objectives are to propose biocontrol methods and new active ingredients, and to evaluate their impacts and limits for the cultivation of almond trees in organic orchards. The project includes 4 research pillars. The first one is to study the possibility of a mass trapping method with kairomones for the almond wasp (Eurytoma amygdali). The second pillar consists of testing the effectiveness of new molecules against almond diseases and pest in producer’s fields. The third pillar consists of evaluating certain cover crops for the management of pests and beneficial insects in almond orchards. The objectives of the last pillar are to identify the environmental factors that favor the development of the almond wasp. In the last year of the project, it is planned to transfer and communicate the innovative strategies resulting from the project to almond growers. The first results show that it would be possible to limit the pressure from almond pest and disease and to produce almond in organic orchards.
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titre
Antixenosis Resistance in Wild and Cultivated Tomato Accessions: How Plant Volatilome Influences the Behavior of Tuta absoluta?
auteur
Komla Exonam Amegan, Yasmine Fourati, Romain Larbat, Bernard Caromel, Anne Violette Lavoir, Christophe Robin, Alan Kergunteuil
article
Séminaire annuel de l'Ecole Doctorale SIRENA, Mar 2023, NANCY, France
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Developing gene editing strategies in Tomato as a method to translate new genetic resistance to viruses.
auteur
Caroline Lebaron, Jean-Luc Gallois
article
19ème Rencontre de Virologie Végétale, Jan 2023, Aussois, France
resume
A major goal of plant breeding is to be able to translate genetic resistances to viruses across species and in the genetic background of choice. In the case of susceptibility factors, it is thought that resistance to viruses can be achieved by genome editing following the inactivation (knock-out) of Susceptibility genes. Here, we will present results on three candidate genes whose role on resistance to viruses have been previously demonstrated in Arabidopsis. We present our strategy to develop gene inactivation, in two different tomato genetic background. This allow us to try to i) develop resistances to pepino mosaic virus and potexvirus, which are a threat to tomato crops worldwide but also ii) to aim at enhancing the durability of resistance to potyvirus driven by an eIF4E weak allele. We describe a general procedure using CRISPR-Cas9 strategy to achieve gene knockout in tomato following agrobacterium mediated plant transformation. The targeting a genomic coding sequence of a gene of interest allows to generate indels following Double Strand Breaks (DSB). We will therefore present the different steps from designing the sequence guide RNA (sgRNA), cloning the binary vector that will express in planta both sgRNA and the Cas9 nuclease, to agrobacterium-mediated plant transformation. Following regeneration, we will present the characterization of heterozygous and homozygous gene mutated alleles. Because our aim is to contribute to plant breeding, we assess both the potential effect on resistance to viruses, but also how knocking-out Susceptibility gene may affect tomato plant development. Indeed, we report several plant and fruit abnormal phenotypes associated with those genes’ knock-out, such as a reduction in plant size or abnormal fruit shape and content. This makes of these line interesting tools to investigate developmental biology in Tomato, but together with preliminary resistance assays, suggests that overall, knocking-out those genes is unlikely to constitute strong sources of resistance for Tomato breeding.
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Cross-adaptation of endive necrotic mosaic potyvirus to different species of the Asteraceae is due to VPg mutations with pleiotropic effects
auteur
Benoît Moury, Marion Szadkowski, Catherine Wipf-Scheibel, William Billaud, Jonathan Gaudin, Gregory Girardot, Patrick Gognalons, Judith Hirsch, Thibaud Jayet, Sylvain Piry, Loup Rimbaud, Alexandra Schoeny, Eric Verdin, Raphaël Foriens
article
19. Rencontres de virologie végétale (RVV 2023), Jan 2023, Aussois, France
resume
Cross-adaptation occurs when adaptation to a novel environment, for example a novel host species, drives also an adaptation to another environment to which the organism was not exposed and that did not act as a selection factor. In natural conditions, endive necrotic mosaic virus (ENMV) is mostly confined to wild salsify (Tragopogon pratensis) and infects occasionally lettuce (Lactuca sativa) crops. To investigate the capacity of viruses to infect novel host species and the associated mechanisms, we performed an experimental evolution of ENMV in five species of the family Asteraceae (L. sativa, T. pratensis, Calendula arvensis, Zinnia elegans or Cichorium endivia). Increase in viral pathogenicity was observed in C. arvensis and Z. elegans only and crossadaptations between C. arvensis and Z. elegans were also noticed. Parallel nonsynonymous substitutions were observed at different nucleotide positions in the VPg cistron of the adapted ENMV populations, and were candidates for the observed pathogenicity changes. Some of these were shared between C. arvensis- and Z. elegans-evolved populations, and could explain the crossadaptations. Using a reverse genetics approach with an infectious cDNA clone of ENMV, we validated that several of these mutations were sufficient to confer adaptation to C. arvensis, Z. elegans or both. This result indicates that the molecular mechanisms involved in host jumps of ENMV, i.e. adaptation to novelspecies, can be similar to those involved in resistance breakdown for other potyviruses, i.e.adaptation to novel genotypes carrying resistance genes within a species, since they involve the same viral protein and, partly, the same protein domains. Using a larger set of species of the Asteraceae, we are now testing the hypothesis that such crossadaptations occur preferentially at the within-tribe level in this botanical family.
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https://hal.inrae.fr/hal-04195740/file/Moury%20et%20al%202023%20-%20Aussois%20poster%20%28abstract%29.pdf BibTex
titre
ESCRT factors: new targets for resistance to potyviruses in crops?
auteur
Aimeric Agaoua, Giuseppe Micali, Luc Sofer, Amandine Bordat, Vincent Simon, Vincent Rittener, Jean-Luc Gallois, Cecile Desbiez, Catherine Dogimont, Sylvie German-Retana
article
19. Rencontres de Virologie Végétale, Jan 2023, Aussois (FR), France. 2022
resume
Endosomal sorting complexes required for transport (ESCRT) play a key role in membrane trafficking and are hijacked by several plant RNA viruses for their replication. In melon, the ESCRT protein CmVPS4 was recently described as a new susceptibility factor to watermelon mosaic virus (WMV, potyvirus) [1]. Indeed, a single amino acid change (P30R) in the CmVPS4 protein is responsible for WMV recessive resistance but the underlying molecular mechanisms are currently unknown. Plant ESCRT proteins have been shown to interact with viral proteins of members of the families Tombusviridae and Bromoviridae. In particular, AtVPS4 protein of A. thaliana interacts directly with the p33 replicase of tomato bushy stunt virus (Tombusviridae) for the formation of viral replication compartment (VRC). We therefore hypothesized that the P30R mutation in CmVPS4 protein in melon could prevent an interaction with a so far undetermined WMV protein. One candidate protein was the potyviral 6K2 that induces the formation of endoplasmic reticulum (ER)-derived viral vesicles crucial for potyvirus replication. Here using Split-ubiquitin membrane Y2H assay, we showed that CmVPS4 interacts with WMV-6K2. We confirmed that the orthologous protein AtVPS4 interacts with TuMV-6K2, in both SuY2H and in planta using BiFC. The 6K2/VPS4 interaction occurs at the VRC level during TuMV infection, suggesting that ESCRT complexes could also play a role in the replication of potyviruses. In the melon/WMV pathosystem, how and where the interaction between CmVPS4 and WMV-6K2 occurs and how the P30R amino acid change is responsible for the resistance are questions that are currently being addressed. [1] DOI: 10.1093/jxb/erac135
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Reports

titre
Fruit crops resilience to climate change in the Mediterranean basin. Freeclimb PRIMA Final report from 28/03/2019 to 27/03/2023
auteur
Bénédicte Quilot-Turion
article
1813-2, Inrae. 2023
resume
The FREECLIMB project is build to match objective 1 of the PRIMA framework in developing smart and sustainable farming systems to maintain natural resources and to increase production efficiency. The project targets major fruit tree species with the aim of improving the availability of breeding and germplasm material adapted to limited external resources (input) and future climatic scenarios in the Mediterranean area, through the characterization and exploitation of local biodiversity. This target will be particularly relevant for Mediterranean agriculture where cropping systems have to cope with preservation (or restoration) of the natural resources in a very constrained environmental context (climate change, water scarcity). The project will focus on key ideotypes elaborated in collaboration with Fruit Farming Actors (FFAs, breeders, nurseries, growers) with the core objective of providing diverse germplasm, tools and methods to accelerate exploitation, breeding and selection of resilient varieties in key traditional fruit crops of Mediterranean agriculture (stone fruits such as peach, apricot and almond; Citrus spp.; grape; olive). To these ends, the project will pursue the following specific objectives: i) developing and applying protocols (e.g. phenotyping methods) and integrated tools (e.g. genotyping methods, data analysis) to support the characterization, exploitation and selection of varieties adapted to a range of agro-ecological and management conditions; ii) dissecting the genetic bases of traits/processes linked to sustainability and plant resilience to biotic and abiotic stress, with particular focus on disentangling genotype x environment x management interaction (GxExM); iii) unravelling the molecular, biochemical and physiological basis of plant adaptation to different environmental (soil and climate) and agronomic conditions (water and fertilizer management) and to biotic/abiotic (single or multiple) stresses; iv) developing and applying genomics-based breeding tools to improve introgression and selection efficiency; v) devising adaptation strategies to cope with the combined effects of multiple stresses occurring under field conditions (e.g heat and drought, pests and diseases); vi) exploiting germplasm resources, by identifying and characterizing spontaneous and domesticated sources of biodiversity; vii) transferring project results through training and dissemination activities dedicated especially to breeders, nurseries and growers, particularly in those countries where fruit production is less developed. Considering southern countries prefigure climate change scenarios predicted for northern ones, FREECLIMB will strongly benefit from collaboration between the south and north Mediterranean shores: for each species targeted by the project at least two countries are involved one from the north and one from the south. The balanced composition of the consortium ensures an equal footing approach with particular attention to co-ownership of results, mutual interest, and shared benefits.
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https://hal.inrae.fr/hal-04172252/file/PRIMA_Final_Report_submitted.pdf BibTex
titre
Host factors involved in potyvirus cell-to-cell movement: new sources of resistance ?
auteur
Sylvie German-Retana, Sébastien Mongrand, Jean-Luc Gallois
article
Inrae. 2023, pp.1-39
resume
How do potyviruses move through plants? The PotyMove project aims to gain a better understanding of the plant factors that enable potyviruses to move from one cell to another. Potyviruses cause severe damage to cultivated plants. Infection is based on a succession of interactions between viral proteins and host proteins. The absence or mutation of a factor essential to the virus leads to plant resistance. This is referred to as passive resistance (preventing a virus function from being carried out), as opposed to active resistance (the plant triggers a panoply of reactions to combat the virus). As they move from cell to cell, viruses exploit plasmodesmata, small channels linking plant cells. Viruses are able to modify these channels in order to move, thanks to the action of viral movement proteins. This step is a real bottleneck for viral infection. Knowing the underlying mechanisms makes it possible to identify new sources of resistance that block or reduce this movement at local level, which at whole-plant level translates into a reduction in viral accumulation, but also a reduction in the risk of the appearance and spread of viral strains bypassing other resistances already present. The PotyMove project therefore aims to gain a better understanding of the plant factors that enable potyviruses to move from one cell to another. Using viral movement proteins as baits to trap their plant partners and validate their role in potyvirus movement During cell-to-cell movement, potyviruses exploit plasmodesmata by producing proteins enabling the passage of the viral genome, known as movement proteins. Using these viral proteins as bait, the aim is to trap their partners in plants, thanks to an original approach of plasmodesmata proteomics and an approach of protein-protein interactions in yeast. The role of the plant factors thus identified (called candidates), in the cell-to-cell transport of potyviruses, must then be validated. This is achieved by obtaining plants mutated for these factors and carefully monitoring the infection of viruses tagged with a fluorescent protein in the mutated plants. In parallel, two complementary genetic approaches are being carried out in the model plant Arabidopsis to identify other candidates based on the observation of plants potentially affected in the movement of the virus, and by identifying the genes responsible. Functional validation of the candidates and/or identification of the genes by genetic approaches are the longest stages of the project. Key project results Two new plant factors have been identified and validated as being involved in potyvirus movement: the Remorin protein, which is found associated with particular membrane domains called lipid rafts in plants, and also in plasmodesms. A second protein (currently being published), partially associated with plasmodesmata and also involved in potyvirus movement, appears to be part of a wider network of proteins capable of modifying specific structures in the plant cell's endoplasmic reticulum. The transfer of these results to tomato is still in progress.
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titre
SENSIVAR. Evaluation de la SENSIbilité des VARiétés de pêches/nectarines et d’abricots aux différents bioagresseurs. Rapport 2021-2022
auteur
Bénédicte Quilot-Turion
article
inrae. 2023
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https://hal.inrae.fr/hal-04172249/file/CR_FAM_SENSIVAR_INRAE_2021-22.pdf BibTex

Preprints, Working Papers, ...

titre
Assessing Inter-Individual Genetic Variability in Peach Sugar Metabolism through Reliable Parameter Estimation of a Kinetic Model
auteur
Hussein Kanso, Mohamed-Mahmoud Memah, Valentina Baldazzi, Bénédicte Quilot-Turion, Charlotte Baey
article
2023
resume
Integrating genetic information into dynamical models is key to understand variations among genotypes and improve crop performances face to current agronomic and ecological constraints. A first and necessary step in building gene-to-phenotype models is the calibration of a large number of genotypes. We compared here two different strategies for the calibration of an Ordinary Differential Equations (ODE) kinetic model simulating the accumulation of different sugars during peach fruit development. First, the model was calibrated for each genotype independently using a Genotype-Based (GB) strategy. Two formulations of the problem have been tested, either as a Single-Objective Optimization (GBS) problem or as a Multi-Objective Optimization (GBM) problem. Second, the model was calibrated for all genotypes simultaneously using a Population-Based (PB) strategy. The two strategies were first applied to a set of simulated data and then to a real dataset derived from an interspecific population of 106 peach genotypes. Results showed that the GB strategy allowed for a high goodness of fit for most genotypes, especially in the GBS formulation. However, the estimated parameters suffered from a lack of practical identifiability as independent repetitions of the estimation algorithm did not always converge to the same value for most genotypes. The PB calibration strategy overcame this issue showing a good identifiability of the population parameter values, a goodness of fit comparable to the one obtained with the GB strategy and a good characterisation of parameter variations within the progeny, which is a key to assess the inter-individual genetic variability. These results are an important step towards the development of reliable gene-to-phenotype models.
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2022

Conference papers

titre
COV de l'amandier : discrimination des émissions au printemps pour la mise au point d'un mélange attractif du ravageur Eurytoma amygdali
auteur
Anjelica Leconte, Philippe Lucas, Nicolas Barthes, Bruno Buatois, Joris Huguenin, Eugénie Coutagne, Henri Duval, Michael Staudt
article
GDR MediatEC annual meeting, Nov 2022, Cargèse, France
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titre
The worldwide population structure of over 3,400 eggplant accessions identified by SPET genotyping
auteur
Lorenzo Barchi, Ezio Portis, Sergio Lanteri, David Alonso, Maria Josè Díez, Jaime Prohens, Véronique Lefebvre, Jeremy Salinier, Hatice Filiz Boyaci, Laura Toppino, Giuseppe Leonardo Rotino, Mark Timothy Rabanus-Wallace, Andreas Boerner, Nils Stein, Richard Finkers, Arnaud G. Bovy, Giuseppe Aprea, Matthijs Brouwer, Roland Schafleitner, Giovanni Giuliano
article
XVII SOLANACEAE2022 International Conference on the Plant Family of Solanaceae, Nov 2022, THESSALONIKI, Greece
resume
G2P-SOL (http://www.g2p-sol.eu) is an EU-funded project, bringing together the main European and international genebanks hosting germplasm of the four major Solanaceous crops: potato, tomato, pepper and eggplant. Within the project, about 5,900 eggplant accessions, including wild relatives of the crop, were inventoried. To provide genomic information on around 3,500 representative accessions, a custom set of 5K SPET probes (single primer enrichment technology, TECAN) was designed targeting SNPs evenly distributed all over the genome but mainly in gene-rich regions. After library preparation, Illumina sequencing and quality filtering, reads were aligned to the eggplant reference genome (version 4.1) using BWA-MEM and SNP calling was performed using GATK4.1.9. By removing accessions with low coverage sequencing data, 3,412 were retained and 120K polymorphic sites identified by applying stringent filtering criteria. Among them, 4,306 were SNPs targeted by the 5K probe set, while the remaining were accessory off-target SNPs. The identified SNPs provided information for identifying putative mislabeled accessions (i.e., wrong species assignment), and duplicates within and between genebanks. Moreover, they are exploitable for setting up screening protocols to avoid the acquisition of duplications by ex-situ maintainers. 34 The population structure and genetic relationships of the eggplant accessions were investigated to build up a core collection for GWAS. Furthermore, based on historical phenotypic data available in genebanks, loci associated with key agronomic traits were identified. Our results demonstrate that SPET technology represents a valid alternative to random complexity reduction methods and arrays, proving a suitable tool for the highthroughput genotyping, management, and enhancement of genebank collections.
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https://hal.inrae.fr/hal-04133464/file/Barchi-SOL2022-Oral%20presentation.pdf BibTex
titre
The genetic architecture of fruit quality and nutritional traits in Solanaceous crops
auteur
Giovanni Giuliano, Véronique Lefebvre, GtoPSOL Consortium
article
XVII SOLANACEAE2022 International Conference on the Plant Family of Solanaceae, Nov 2022, THESSALONIKI, Greece
resume
In the frame of the G2P-SOL project, core collections representative of the worldwide genetic diversity of the four major Solanaceous crops (tomato, pepper, eggplant, potato) were created. These collections were phenotyped and subjected to metabolic profiling. Hundreds of novel metabolites have been identified and quantified, and QTLs have been mapped, both in the core collections and in segregating populations. The genetic architecture of some of these traits will be discussed in detail.
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https://hal.inrae.fr/hal-04136950/file/Giuliano-SOL2022-InvitedTalk.pdf BibTex
titre
Towards understanding of metabolic diversity of pepper fruit
auteur
Yuri Tikunov, Louis McLeod, Giorgio Tumino, Y Wu, Jérémy Salinier, Lorenzo Barchi, Roland Schafleitner, Richard Finkers, Mathjis Brouwer, Felice Boyaci, Gancho Pasev, Ilan Paran, Nils Stein, Jaime Prohens, Giovanni Giuliano, Pasquale Tripodi, Véronique Lefebvre, Arnaud Bovy
article
XVII SOLANACEAE2022 International Conference on the Plant Family of Solanaceae, Nov 2022, THESSALONIKI, Greece
resume
Among the Solanacea crops pepper-Capsicum spp-is the only one that can make one either smile or cry owing to presence of specific metabolites which pepper fruits accumulate. Diverse and recognizable flavor of bell pepper or chilli pepper is determined by specific volatile compounds, pungency, caused by capsaicinoids, is essencial in some 44 World's cuisines, fruit color is determined by carotenoids and flavonoids, nutritional quality-by vitamins and antioxidants. World's gene banks possess a great genetic and phenotypic diversity of pepper that could be used to diversify the cultivated pepper germplasm. To study how the genetic diversity of the most cultivated pepper species C. annuum translates into metabolic diversity ripe fruits of a core collection of 450 accessions developed in the EU Horizon 2020 G2P-SOL project (http://www.g2psol.eu/) were profiled using different metabolomics platforms for volatile aroma compounds using GC-MS and for non-volatile secondary metabolites using LC-MS. Wide qualitative and quantitate metabolic variation was observed. Volatile esters, alkanes, terpenes and oxylipin volatiles determined the variation in the composition of aroma compounds. Flavonoids and capsaicinoids had the major contribution to the variation in semi-polar secondary metabolite composition. Compounds from the abovementioned groups revealed highly similar quantitative patterns across the core collection suggesting a coordinate regulation of compounds that belong to the same biosynthetic origin. GWAS was performed using SNP data obtained using genotyping-by-sequencing and revealed multiple associations of the metabolic variation with genomic loci harboring known and novel candidate genes.
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https://hal.inrae.fr/hal-04133409/file/Tikunov-SOL2022-Oral%20presentation.pdf BibTex
titre
SPET genotyping of over 15,000 accessions of tomato reveals the genetic structure and history of the tomato germplasm at world level
auteur
Jose Blanca, Giuseppe Aprea, Paola Ferrante, V.V. Fain, David Alonso, Lorenzo Barchi, Roland Schafleitner, R. Brandt, Mark Timothy Wallace, Andreas Böerner, Nils Stein, Jérémy Salinier, Véronique Lefebvre, Arnaud Bovy, Filiz Boyaci Hatice, Gancho Pasev, Ivanoka Tringovska, Dani Zamir, Jaime Prohens, J Canizares, Maria Jose Díez, Giovanni Giuliano
article
XVII SOLANACEAE2022 International Conference on the Plant Family of Solanaceae, Nov 2022, THESSALONIKI, Greece
resume
Tens of thousands tomato accessions are conserved in genebanks all over the world, constituting a valuable source of genes of interest for tomato breeding. Genetic characterization of such large collections can improve their management. The G2P-SOL project has carried out the largest-scale tomato genotyping to date, by designing a 5k probe Single Primer Enrichment (SPET) tomato platform, and used it to genotype a collection of over 15,000 accessions from worldwide genebanks. Besides wild species, the main genetic groups found were: wild S. lycopersicum var. cerasiforme (SLC) from Meso-and Southamerica, early vintage cultivated tomatoes, that genetically are closer to SLC than to the bulk of S. lycopersicum var. lycopersicum (SLL), vintage tomatoes, mainly from Spain, early improved materials with no introgressions, and more recent materials that do include wild species introgressions. The structure of the early vintage 36 accessions mirrored the early history of the cultivated tomatoes. They were widely spread across Meso-and South America, probably because they were the cultivated tomatoes in those regions before the Spanish arrival. Interestingly, they were also found in other geographic regions controlled or influenced by the Spaniards during the between XVII, and XVIII centuries, such as the western Pacific (mainly the Philippines) and the Mediterranean. History has left many marks in the tomato genomes. For instance, the Italian San Marzano is genetically closer to a population of ovoid brasilian early breeds that genetically are better characterized as SLC than to most European vintage tomatoes, that are SLL.
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Identification des COV actifs chez les femelles Eurytoma amygdali
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Anjelica Leconte, Bruno Buatois, Nicolas Barthes, Eugénie Coutagne, Henri Duval, Michael Staudt, Philippe Lucas
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7ème rencontre annuelle du GDR O3, Oct 2022, Paris, France
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Levier génétique sur abricotier et pêcher: où en sommes-nous?
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Morgane Roth
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Journée protection sanitaire fruits à noyau, Sudexpé - Station de recherche appliquée Fruits et Légumes, Oct 2022, Saint Gilles, France
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Genome-wide association study of salt tolerance in tomato
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Alexandre Héreil, Maxime Guillaume, F. Bitton, Mathilde Causse
article
Solanaceae genome 2022, Thessaloniki University, Oct 2022, Thessaloniki, Greece
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Salinity is a common abiotic stress in many tomato production areas due to the scarcity of fresh water in arid and coastal regions of the world. Therefore, it is necessary to understand the mechanisms underlying genetic diversity for salt sensitivity in order to breed new tolerant cultivars. Several quantitative trait loci (QTLs) related to abiotic stress tolerance have been identified in tomato using genome wide association studies and a few genes underlying them have been successfully identified. In our study, we analysed a whole-genome sequenced panel of 150 tomato genotypes composed of wild accessions (S. pimpinellifollium), cherry tomatoes (S. cerasiforme) and admixed accessions. This panel was phenotyped during early growth and adult stages for both control and salt-stressed treatments. The characterisation of salinity tolerance was achieved on several traits including mineral contents, metabolomics and agronomic traits. A large variability in response was observed for most of the traits and a few tolerant varieties were identified. Then we conducted a genome-wide association study on these traits and identified dozens of QTLs. Several candidate genes within these QTLs have functions related to salinity stress tolerance. Some of them are consistent with previously published QTLs. These results provide new insights into the genetic architecture of tomato salt tolerance and provides new genes to be functionally validated.
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Genetic control of tomato fruit quality : from GWAS to breeding
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Mathilde Causse, Estelle Bineau, Jiantao Zhao, Jose-Luis Rambla, Juliette Benejam, Marie Brault, Yolande Carretero, Esther Pelpoir, Karine Pellegrino, Antonio Granell
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Solanaceae genome 2022, Thessaloniki University, Oct 2022, Thessaloniki, Greece
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Tomato flavor has changed over the course of domestication and later during breeding although it was not a target for breeders until recently. For years consumers complained about the taste of modern varieties. Flavour and nutritional quality thus became important goals for tomato breeders. Although tomato taste is influenced by environmental and post-harvest conditions, the varieties show a large diversity for sensory traits as welle as fruit composition. We will present our recent work intended to improve tomato fruit quality. Since the availability of large numbers of SNP, genome-wide association studies (GWAS) were performed on several panels of tomato lines. We thus performed a meta-analysis of GWAS for 18 traits, combining results on 775 tomato accessions and 2,316,117 SNPs from three GWAS panels. We discovered 305 significant associations for the contents of sugars, acids, amino acids and flavor-related volatiles. We showed for instance that fruit citrate and malate contents have been impacted by selection during domestication and improvement, while sugar content has undergone less stringent selection. Volatile organic compound contents also evolved and some trends for improvement were identified. More recently we checked the inheritance of volatiles at the hybrid level in order to help the production of F1 hybrids with good quality. We will discuss how to use these data to improve tomato quality.
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Phenomic data analysis and management: example in the European community
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Emilie J. Millet, Llorenç Cabrera-Bosquet, Diana Pérez-Valencia, Martin Boer, María Rodríguez-Álvarez, Ítalo Stefanine Correia Granato, Claude Welcker, François Tardieu, Fred A. van Eeuwijk
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7th International Plant Phenotyping Symposium ‘Plant Phenotyping for a Sustainable Future', WUR, Sep 2022, Wageningen, Netherlands
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Studying G×E to dissect plant responses to environmental changes: integration of methods and data
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Emilie J Millet, Diana Pérez-Valencia, María Rodríguez-Álvarez, Martin Boer, Andreas Hund, Lukas Kronenberg, Willem Kruijer, Aude Coupel-Ledru, Ítalo Stefanine Correia Granato, Alain Charcosset, Claude Welcker, François Tardieu, Fred A. van Eeuwijk
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XVIIIth Eucarpia Biometrics in Plant Breeding Conference, Hans-Peter Piepho; Laurence Moreau; Tristan Mary-Huard, Sep 2022, Gif-sur-Yvette, France
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Robustesse de l'immunité du piment à Phytophthora capsici face à une perturbation thermique
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William Billaud, Thibaud Jayet, Valentin Ribaut, Lucie Tamisier, Anne Massire, Bernard Caromel, Sonia Elbelt, Jacques Lagnel, Judith Hirsch, Félicie Lopez-Lauri, Benoît Moury, Véronique Lefebvre
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13. Rencontres de Phytopathologie - Mycologie, Sep 2022, Aussois (France), France. 1p
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La réponse des plantes à l’attaque d’un agent pathogène est dépendante de l’environnement dans lequel elle se trouve. Dans un contexte de changement climatique, et donc de forte augmentation de la variabilité environnementale, il est difficile de prédire la réponse des plantes. Il est alors important de développer des variétés dont le phénotype est stable quel que soit l’environnement de culture. Ce phénomène correspond à la robustesse, qui est définie par une variation faible, voire nulle, d’un trait face à une perturbation donnée. Ce concept, à l’origine identifié chez les animaux, est encore peu étudié chez les plantes. Identifier les régions du génome contrôlant la robustesse d’un trait serait une grande avancée pour l’amélioration des plantes, permettant par exemple de créer des variétés dont l’immunité serait stable. Nos recherches s’intéressent à la robustesse de l’immunité du piment face à une perturbation thermique. Nous avons pour objectifs principaux de (i) définir des méthodes d’estimation de la robustesse d’un trait et (ii) d’identifier les régions du génome qui y sont associées. Pour cela, nous avons mesuré l’immunité d’une collection représentative de la diversité génétique du piment (Capsicum annuum L.) à Phytophthora capsici, dans deux environnements thermiques contrastés. Plusieurs estimateurs de la robustesse de l’immunité entre les deux environnements testés ont été comparés entre eux. L’immunité au sein de chaque environnement est également étudiée. L’obtention, en cours, d’une carte de marqueurs SNP (single-nucleotide polymorphisms) à haute densité par capture de gènes permettra d’identifier, par génétique d’association (genome-wide association studies ou GWAS), les régions du génome du piment responsables de (i) l’immunité à Phytophthora capsici sous chacun des deux environnements testés et (ii) la robustesse de l’immunité face à une perturbation thermique.
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https://hal.inrae.fr/hal-04133317/file/JJC%20AUSSOIS%202022%20Mycologie-WBillaud-soumis.pdf BibTex
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Screening for multi-pest symptoms in an apricot, and a peach core collection
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Marie Serrie, Alain Blanc, Laurent Brun, Guy Clauzel, Frédéric Combe, Frédéric Gilles, Guillaume Roch, Véronique Signoret, Sabrina Viret, Jean Marc Audergon, Bénédicte Quilot-Turion, Morgane Roth
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International Horticultural Congress - IHC 2022, Aug 2022, Angers (FR), France
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Screening local landraces of melon for resilience to salinity stress under hydroponic greenhouse conditions
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T. Ntanasi, G. Ntatsi, I. Karavidas, G. Outos, G. Maloukos, V. Fotopoulos, M. Guillaume, Mathilde Causse, M.J. Clemente, A. Schubert, J. Galmès, M. Conesa, D. Savvas
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31st International Horticultural Congress (IHC) - Horticulture for a World in Transition / International Symposium on Adaptation of Horticultural Plants to Abiotic Stresses, Aug 2022, Angers, France. pp.309-316, ⟨10.17660/ActaHortic.2023.1372.40⟩
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Water shortage and heat driven evaporation in semi-arid climate, such as Mediterranean, that are mainly ascribed to climate change, introduced salinity as a major stress factor in vegetable crop production. Thus, the adaptation of vegetables, especially those susceptible to osmotic stress, is becoming more and more challenging to acquire sustainable cropping systems in saline environment. Unlike melon hybrids, melon landraces are less sensitive to environmental stresses and considered valuable sources of genetic characteristics for plant breeders' interest in breeding programs. This study was designed to assess the impact of salinity on several fruit quality characteristics of three melon landraces namely 'Tendra negro, 'Thrakiotiko' and 'Leyko Amyntaiou'. Both, the widely cultivated Spanish cultivar 'T111 (tipo piel de sapo)' and the commercial hybrid 'Lavigal' served as control. The experiment was carried out at the greenhouse facilities of the Laboratory of Vegetable Production at the Agricultural University of Athens. The different melon genotypes were hydroponically cultivated in an open hydroponic system with perlite as substrate, by applying a nutrient solution of 0.5 mM NaC1 to the non-saline treated plants and 30 mM NaC1 to the salt exposed plants. To investigate the impact of the salt stress on fruit quality, parameters such as the dry matter content, the pulp firmness, the values of color parameters L* a*, b*, hue angle and chroma and the titratable acidity were determined. The results showed that the color parameters and the pulp firmness were cultivar depended and were not affected by the salinity stress applied. Moreover, salinity stress resulted in increased dry matter content of the landrace 'Thrakiotiko, increased titratable acidity of the landrace 'Leyko Amyntaiou'. However, all tested parameters for the 'Tendra negro' landrace were not affected by the stress applied, indicating that this landrace could be a good candidate to be used in breeding programs for improving the tolerance of the commercial melon to salt stress.
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Potato virus Y adaptation to various resistance QTL combinations in pepper and impact on host tolerance
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Thibaud Jayet, William Billaud, Lucie Tamisier, Marion Szadkowski, Catherine Wipf-Scheibel, Loup Rimbaud, Judith Hirsch, Gregory Girardot, Jonathan Gaudin, Alexandra Schoeny, Karine Berthier, Karine Nozeran, Elise Lepage, Sarah de Colle-Guihéneux, Thomas Pressecq, Eric Verdin, Véronique Lefebvre, Benoît Moury
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15. International symposium of plant virus epidemiology, Consejo Superior de Investigaciones Científicas, Madrid, spain, Jun 2022, Madrid, Spain
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Specificity of resistance and tolerance to cucumber vein yellowing virus in melon accessions and evidence for resistance-breaking associated with a single mutation in VPg
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Cecile Desbiez, Maria Luisa Domingo Calap, Michel Pitrat, Catherine Wipf-Scheibel, Gregory Girardot, Immaculada Ferriol, Juan López-Moya, Hervé Lecoq
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15. International symposium of plant virus epidemiology, Consejo Superior de Investigaciones Científicas, Madrid, spain, Jun 2022, Madrid, Spain
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Change of paradigm for breeding disease resistant cultivars robust to varying environments: estimators of the robustness of plant immunity and promising genes for the future
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William Billaud, Thibaud Jayet, Valentin Ribaut, Lucie Tamisier, Anne Massire, Marion Szadkowski, Sarah de Colle-Guihéneux, Bernard Caromel, Sonia Elbelt, Jacques Lagnel, Judith Hirsch, Félicie Lauri, Benoît Moury, Véronique Lefebvre
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European Scientific Conference – Towards Pesticide Free Agriculture “What research to meet the pesticides reduction objectives embedded in the European Green Deal?”, French Research Institute for Agriculture, Food and Environment (INRAE), Jun 2022, Dijon, France
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Genetic control of tomato fruit quality : from GWAS to breeding
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Mathilde Causse, Estelle Bineau, Jiantao Zhao, Juliette Benejam, Marie Brault, Yolande Carretero, Esther Pelpoir, Karine Pellegrino
article
EUCARPIA Tomato congress, May 2022, VALENCIA, Spain
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Tomato flavor has changed over the course of domestication and later during breeding although it was not a target for breeders until recently. Consumers then complained about the taste of modern varieties. Although tomato taste is influenced by environmental and post-harvest conditions, the varieties show a large diversity for fruit composition and texture. We will present our recent work intended to improve tomato fruit quality. Since the availability of large numbers of SNP, genome-wide association studies (GWAS) were performed on several panels of tomato lines. We thus performed a meta-analysis of GWAS for 18 traits, combining results on 775 tomato accessions and 2,316,117 SNPs from three GWAS panels. We discovered 305 significant associations for the contents of sugars, acids, amino acids and flavor-related volatiles. We showed for instance that fruit citrate and malate contents have been impacted by selection during domestication and improvement, while sugar content has undergone less stringent selection. Volatile organic compound contents also evolved and some trends for improvement were identified. More recently we checked the inheritance of volatiles at the hybrid level in order to help the production of F1 hybrids with good quality. We are now assessing how to use these data to improve tomato quality.
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Multi-trait genomic prediction to improve tomato aroma contents
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Juliette Bénéjam, Estelle Bineau, Alexandre Hereil, Henri Desaint, Mathilde Causse
article
EUCARPIA Tomato congress, May 2022, VALENCIA, Spain
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The increasing consumer demand for tastier tomatoes led to the development of several breeding projects for quality. The first ones are based on increasing the sugar content and improving the acid balance (malic and citric acids) as both traits have been widely described as the main contributors to tomato flavor. However, sugar content and fruit weight are primarily regulated by linked genetic regions with opposite effect, so increasing sugar content reduces fruit weight. In this context, other breeding levers have been considered: the most promising is the diversification of tomato aromas. Tomato aromas are combinations of large number of volatile compounds among which 30 have a major effect on taste. Each of these is controlled by a large number of QTLs. Genomic prediction is a statistical method to estimate the breeding value of genotypes based on their genotyping information. It is well suited for the improvement of low effect polygenic traits. Thus, we explored the efficiency of this method for aroma content prediction on GWAS data previously published by Bineau et al. (2021): 44 volatiles assessed by GS-MS in a collection of 121 cherry tomato lines. The ability and accuracy of prediction models adapted to multiple traits were tested by cross-validation in comparison with single trait models. The second challenge was to predict the breeding values of hybrids (derived from the line collection). Third, we explored some index based on combined volatiles content representing aroma to predict global tomato flavour. I will present the latest results on these three axes.
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Improving genomic predictions with inbreeding and non-additive effects in two admixed maize hybrid populations in single and multi-environment contexts
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Laurence Moreau, Morgane Roth, Aurélien Beugnot, Tristan Mary-Huard, Alain Charcosset, Julie B Fiévet
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XXVth EUCARPIA Maize and Sorghum Conference, May 2022, Belgrade, Serbia
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In maize (Zea mays), hybrid selection has led to the structuration of genetic diversity into complementary heterotic groups. This structuration is convenient for creating good performing hybrids but the reduced gene flow between groups increases the risk of intra-group genetic erosion associated with drift and selection, which may hinder long term breeding efficiency. It also complexifies the introduction of diversity from material such as landraces or admixed individuals derived from intergroup hybrids, that cannot be assigned to a specific heterotic group. Crossing admixed lines can generate contrasted levels of inbreeding in the hybrid population. This can reveal substantial non-additive effects, which impact performance and variation for traits showing heterosis These non-additive effects raise specific issues for the implementation of genomic selection. Recent methodological developments modelling inbreeding and non-additive effects with the natural and orthogonal interaction approach (NOIA) have been proposed in order to increase genomic prediction accuracy in hybrid populations. Using two maize populations of hybrids admixed between dent and flint heterotic groups, we compared genomic prediction models incorporating (or not) inbreeding and non-additive effects in single and multi-environment contexts. Both populations showed a strong impact of inbreeding on plant yield, height and flowering time, which was supported by the superiority of prediction models incorporating this effect. The model including additivity, dominance, epistasis and inbreeding effects appeared to be the most robust for prediction across traits and populations and was advantageous when predicting hybrids not observed yet in any environment. Overall, comparing variance decompositions was helpful to guide model selection for genomic prediction. Finally, we recommend the use of models including inbreeding and non-additive parameters following the NOIA approach to increase prediction accuracy in admixed populations. Based on these results we will discuss the opportunities offered by genomic selection to revisit maize hybrid breeding.
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Cucurbit attractivity to Aphis gossypii is under polygenic control
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Severine Monnot, Eva Coindre, Pascale Mistral, Karine Leyre, Joel Chadoeuf, Nathalie Boissot
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12e réunion annuelle du réseau BAPOA, May 2022, Nice, France
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Sélection pour des idéotypes résilients chez les Prunus
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Morgane Roth
article
Animation scientifique « La résilience des plantes face aux variations de leur environnement », Plant Alliance, Mar 2022, En ligne, France
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The Human History of Chilli Peppers Told by 10,000 Genebank Samples
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Mark Timothy Rabanus-Wallace, Pasquale Tripodi, Lorenzo Barchi, Sandip Mallikarjun Kale, Alberto Acquadro, Roland Schafleitner, Jaime Prohens, Maria José Diez, Andreas Boerner, Jérémy Salinier, Bernard Caromel, Arnaud G. Bovy, Filiz Boyaci, Ronny R. Brandt, Axel Himmelbach, Maarten van Zonneveld, Salvatore Esposito, Gancho Pasev, Ezio Portis, Richard Finkers, Sergio Lanteri, Ilan Paran, Véronique Lefebvre, Giovanni Giuliano, Nils Stein
article
Virtual Plant and Animal Genome XXIX Conference, Jan 2022, San Diego (CA), United States
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From vindaloo to ratatouille, kung pao to Tex-Mex, chilli peppers and capsicums (Capsicum spp.) are valued staples in cuisines across the globe, prized for their garish aesthetics, diverse flavours, and pungency. First domesticated in South-and Mesoamerica, the Age of Discovery began a dynamic global trade in peppers, resulting in a highly intermixed distribution of pepper varieties across regions of the globe. This high degree of inter-region overlap makes genetic inference on pepper's history a unique challenge, especially with limited sample numbers. Fortunately, genebanks across the globe preserve an excellent representation of pepper diversity in different regions, and the G2P-Sol project (EU) recently completed the GBS sequencing of over 10,000 such accessions. Leveraging this vast dataset, we developed the novel method ReMIXTURE ("Regional Mixture") to complement traditional methods and allow insight into how the kinds of peppers cultivated in various regions overlap with those from various other regions. Despite low genetic differentiation, we were able to detect the signatures of transatlantic maritime trade routes, overland trade in the Americas and Eurasia, and the role of Africa as an important node joining the Americas to the East. The varying degrees of uniqueness of peppers found in each region were quantified, revealing hotspots of potential diversity (Mesoamerica, Southeast Asia, and Africa in particular) exploitable by breeders with an interest in introducing novel genetic variants and phenotypes to breeding efforts. The study also allowed us to assess aspects of genebank management such as duplicate detection, and to identify the influence of recent selective pressures on genes affecting fruit pungency and other qualities.
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https://hal.inrae.fr/hal-03551615/file/Rabanus-Wallace%202022%20PAG%20San%20Diego%20-%20oral.pdf BibTex
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Worldwide Population Structure of Eggplant Identified by SPET Genotyping over 3,400 Accessions
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Lorenzo Barchi, Ezio Portis, Sergio Lanteri, David Alonso, Maria José Diez, Jaime Prohens, Véronique Lefebvre, Jérémy Salinier, Hatice Filiz Boyaci, Laura Toppino, Giuseppe Leonardo, Mark Timothy Rabanus-Wallace, Andreas Boerner, Nils N. Stein, Richard Finkers, Matthijs Brouwer, Arnaud G. Bovy, Roland Schafleitner, Giuseppe Aprea, Giovanni Giuliano
article
Virtual Plant and Animal Genome XXIX Conference, Jan 2022, San Diego (CA), United States
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G2P-SOL (http://www.g2p-sol.eu) is an EU-funded project, bringing together the main European and international genebanks hosting germplasm of the four major Solanaceous crops: potato, tomato, pepper and eggplant. Within the project, about 5,900 eggplant accessions, including wild relatives of the crop, have been inventoried. To provide genomic information on around 3,500 representative accessions, a custom set of 5K SPET probes (single primer enrichment technology, TECAN) was designed targeting SNPs evenly distributed all over the genome but mainly in gene-rich regions. DNA samples were prepared by the genebanks, while library preparation and Illumina sequencing was performed by IGA Technology Services. After quality filtering, reads were aligned to the eggplant reference genome sequence (version 4.1) using BWA-MEM and SNP calling was performed using GATK-4.1.9. By removing accessions with low coverage sequencing data, 3,412 were retained and 120K polymorphic sites identified by applying stringent filtering criteria. Among them, 4,306 were SNPs targeted by the 5K probe set, while the remaining were accessory off-target SNPs. The identified SNPs provided information for identifying putative mislabeled accessions (i.e., wrong species assignment), duplicates within and between genebanks. Moreover, they are exploitable for setting up screening protocols to avoid the acquisition of duplications by ex-situ maintainers. The population structure and genetic relationships of the eggplant accessions were investigated to build up a core collection for GWAS. Furthermore, based on historical phenotypic data available in genebanks, loci associated with key agronomic traits were identified. Our results demonstrate that SPET technology represents a valid alternative to random complexity reduction methods and arrays, proving a suitable tool for the high-throughput genotyping, management, and enhancement of genebank collections.
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Poster communications

titre
A reference genome of Solanum habrochaites
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Bernard Caromel, Jacques Lagnel, Carole Iampietro, Marie-Claire Le Coant, Marine Sallaberry, Renaud Duboscq, Alexandre Bachellez, Anne Massire, Olivier Bouchez, Jérôme Lluch, Véronique Lefebvre
article
XVII SOLANACEAE2022 International Conference on the Plant Family of Solanaceae, Nov 2022, THESSALONIKI, Greece
resume
Related species have been used in tomato breeding as sources of resistance to many diseases. Genetic maps based on progenies issued from interspecific crosses highlighted chromosomal rearrangements between the cultivated tomato (Solanum lycopersicum) and related species. Genome rearrangements limit therefore the use of the tomato reference genome sequence to study interesting loci in related species. High-quality genomes from tomato-related species are available for S. pimpinellifolium and S. pennellii. However, no high-quality genome is available to date for S. habrochaites, a species carrying resistance to several pests and pathogens. We thus constructed a reference genome of S. habrochaites, using a combination of PacBio Hifi sequencing and Hi-C technology for scaffolding. Following the PacBio Hifi sequencing, we assembled the genome with a total size of 1.03Gbp in 959 contigs, with N50 of 14 Mb, using Hifiasm software. Then, the contigs were scaffolded using Hi-C data to obtain chromosome-scale pseudo-molecules. Finally, the assembly was polished using Illumina short reads. We aim to use this novel reference genome of S. habrochaites to accurately identify resistance QTLs in regions rearranged between the genomes of S. habrochaites and of cultivated tomato.
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https://hal.inrae.fr/hal-04136943/file/Caromel-SOL2022-Poster%20presentation.pdf BibTex
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Projet Cap Zéro Phyto
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Hélène Gautier, Bernard Caromel, Komla Amegan, Anne Massire, Marie-Claire Le Coant, Marc Bardin, Philippe C. Nicot, Jean-François Bourgeay, Magali Duffaud, Anne Violette Lavoir, Romain Larbat, Alan Kergunteuil, Jawad Aarrouf, Laurent Urban
article
Med'agri - Le salon de l'agriculture méditerranéenne, Oct 2022, Avignon, France.
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Débuté en 2021, le projet Cap Zéro Phyto financé par le PPR « Cultiver et protéger autrement » a pour objectif d’étudier indépendamment cinq leviers immunitaires et leurs combinaisons pour protéger notamment les cultures de tomate contre leurs principaux bioagresseurs (la mouche Tuta absoluta, le champignon responsable de la pourriture grise Botrytis cinerea, l’oïdium de la tomate Oidium neolycopersici, l’agent du mildiou Phytophthora infestans et les nématodes).
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https://hal.inrae.fr/hal-03963180/file/INRAE_Projet%20Cap%20Z%C3%A9ro%20Phyto.pdf BibTex
titre
Genetic analysis of tomato response to low Nitrogen conditions
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Henri Desaint, Alexandre Héreil, Yolande Carretero, Esther Pelpoir, Renaud Duboscq, Mathilde Causse
article
Solanaceae genome 2022, Oct 2022, Thessaloniki, Greece
resume
Half of the world population is supported by the use of synthetic fertilizers, nitrogen (N) in all its forms being prominent among them. However, the production and utilization of nitrogen fertilizers have well established detrimental effects on global changes. Notably, the excessive use which impacts water resources and imbalance micro-organisms equilibrium. Therefore, minimizing N input, either through efficient fertilizer management or the identification of high N-use efficient (NUE) genotypes is one of the main challenges to sustain high crop yields while reducing environmental costs. Our study is part of the SusCrop ERA-NET SOLNUE project aiming to provide knowledge and strategies toward N fertilizer reduction on tomato and eggplant, emphasising the genetic solutions. We focus here on tomato with two goals (i) identifying QTLs involved in NUE, N storage or response to N variation in a diversity panel phenotyped under to N fertilization conditions and (ii) highlight the transcriptional regulation of tomato response to N limitation in contrasting lines and hybrids, in leaves and fruits. First results show a significant negative impact of N stress on biomass production while no clear effect was seen on fruit quality. Furthermore, it seems the N stress induces more regulation in leaves compared to fruits with around 20% of the differentially expressed genes (DEG) in common between the two organs. Finally, the level of regulation varies between genotypes and organs.
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Improving genomic predictions with inbreeding and non-additive effects using two hybrid admixed populations in maize
auteur
Morgane Roth, Aurélien Beugnot, Tristan Mary-Huard, Laurence Moreau, Alain Charcosset, Julie Fiévet
article
Eucarpia Biometrics, Sep 2022, Gif-sur-Yvette, France. 2022
resume
Genetic admixture, resulting from the recombination between structural groups, is frequently encountered in breeding populations. In hybrid breeding, crossing admixed lines can generate substantial non-additive genetic variance and contrasted levels of inbreeding which can impact trait variation. This study aimed at testing recent methodological developments for the modelling of inbreeding and non-additive effects in order to increase prediction accuracy in admixed populations. Using two maize (Zea mays) populations of hybrids admixed between dent and flint heterotic groups, we compared a suite of five genomic prediction models incorporating (or not) parameters accounting for inbreeding and non-additive effects with the natural and orthogonal interaction approach (NOIA) in single and multi-environment contexts. In both populations, variance decompositions showed the strong impact of inbreeding on plant yield, height and flowering time which was supported by the superiority of prediction models incorporating this effect (+0.038 in predictive ability for mean yield). In most cases dominance variance was reduced when inbreeding was accounted for. The model including additivity, dominance, epistasis and inbreeding effects appeared to be the most robust for prediction across traits and populations (+0.054 in predictive ability for mean yield). In a multi-environment context, we found that the inclusion of non-additive and inbreeding effects was advantageous when predicting hybrids not yet observed in any environment. Overall, comparing variance decompositions was helpful to guide model selection for genomic prediction. Finally, we recommend the use of models including inbreeding and non-additive parameters following the NOIA approach to increase prediction accuracy in admixed populations.
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.science/hal-04132668/file/Biometrics22_2.pdf BibTex
titre
Estudio genético de 15.504 accesiones de tomate y establecimiento y caracterización de la colección nuclear resultante
auteur
David Alonso, Giuseppe Aprea, Paola Ferrante, Fain V. Vanessa, Cristina Casanova, María Figás, Salvador Soler, Ivanka Tringovska, Stanislava Grozeva, Daniela Ganeva, Gancho Passev, Olivia Demurtas, Manuela Costanzo, Saleh Alseekh, Alisdair Robert Fernie, Jose Luis Rambla, Antonio Granell, Lorenzo Barchi, Roland Schafleitner, Andreas Börner, Jérémy Salinier, Arnaud Bovy, Véronique Lefebvre, Filiz Boyaci, Nils Stein, Jaime Prohens, Maria José Diez Niclos, Giovanni Giuliano
article
X CONGRESO DE MEJORA GENÉTICA DE PLANTAS, Sep 2022, Pontevedra, España
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03737981/file/2022%20Poster%20Abstract_Alonso%20D-Spain-TomatoDiversityG2P-SOL.pdf BibTex
titre
Impact de la résistance partielle du piment sur l'expression des gènes de Phytophthora capsici : conséquences pour la création variétale
auteur
Véronique Lefebvre, Anne Massire, Gaëtan Maillot, Emmanuel Szadkowski, Bernard Caromel, Alexandre Bachellez, Nasradin Touhami, Véronique Brunaud, Guillem Rigaill, Kurt Lamour
article
13e Rencontres de Phytopathologie - Mycologie, Journées Jean Chevaugeon, Sep 2022, Aussois (France), France
resume
La résistance des plantes aux agents pathogènes est qualitative lorsque la progression de la maladie est stoppée ou quantitative lorsque le développement de l’agent pathogène est réduit. Le déploiement des résistances qualitatives dans les agrosystèmes peut exercer une forte pression de sélection sur l’agent pathogène ciblé et aboutit fréquemment à la sélection de variants virulents. En revanche, les résistances quantitatives exercent une pression sélective moindre et sont souvent plus durables. Or l’impact des résistances quantitatives sur les mécanismes moléculaires associés à la pathogénicité est encore mal compris. Ainsi, nous présenterons une analyse du transcriptome in planta de deux isolats de Phytophthora capsici, l'un adapté au piment et l'autre non adapté. Nous décrirons comment des plantes hôtes, sensibles ou partiellement résistantes, modifient la régulation des gènes de P. capsici aux premiers stades de l'interaction. Parmi les gènes différentiellement exprimés par P. capsici entre les quatre interactions hôtes-isolats étudiées, nous avons identifié des gènes codant pour des transporteurs de substances nutritives et inorganiques et des gènes codant des hydrolases, suggérant leur rôle dans le développement de P. capsici qui puise ses nutriments chez l’hôte sensible après avoir dégradé la paroi cellulaire de la plante. L'analyse RNA-seq a également mis en évidence des effecteurs cytoplasmiques RxLR dont l'expression varie soit en fonction de l'isolat, soit en fonction de l’hôte. Nos résultats fournissent un répertoire de gènes de P. capsici régulés au cours de son interaction avec le piment. Le sélectionneur visera alors à créer des variétés de piment qui confèrent un état de famine chez l’agent pathogène.
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04133322/file/JJC%20AUSSOIS%202022%20Mycologie-VLefebvre-soumis.pdf BibTex
titre
Genetic architecture of powdery mildew resistance in pepper and impact of genetic resistance factors on pathogen diversity
auteur
Flavie Cussonneau, Carole Constant, Schalk Van Heerden, Marc Bardin, Benoît Moury, Véronique Lefebvre
article
13. Rencontres de Phytopathologie - Mycologie, Sep 2022, Aussois (France), France
resume
The ascomycete Leveillula taurica causes powdery mildew of pepper, resulting in yield losses of up to 40%. This obligate biotrophic fungus has a wide host range including different plant families such as Solanaceae, Asteraceae and Cucurbitaceae. Molecular diversity studies of this pathogen have revealed different clades distributed by host species [1]. On the host side, both monogenic and polygenic resistance to L. taurica have been described in pepper (Capsicum spp.). However, the identified resistances remain either ineffective under heavy infection or the underlying genetic determinants are insufficiently mapped for breeding. Therefore, we aim to (i) establish a catalogue of resistance loci to L. taurica, based on the diversity of Capsicum annuum, (ii) characterise the genetic and phenotypic diversity of L. taurica worldwide, and (iii) analyse the effect of pepper resistance loci to L. taurica on its diversity and that of other pathogens. The catalogue of pepper resistance loci will be based on linkage analysis in progenies and in two core collections by Genome Wide Association Studies. We will collect a large set of L. taurica isolates from more than fifteen sites worldwide and assess their aggressiveness on three pepper genotypes contrasted for resistance. In addition, description of L. taurica genetic diversity will rely on de novo sequencing that will allow building a new reference genome and deriving a SNP matrix in order to genotype the collected isolates. Finally, we will characterize population dynamics not only of L. taurica, but also of the oomycete Phytophthora capsici and the viruses CMV (Cucumber mosaic virus) and PVY (Potato virus Y) on a set of lines contrasted for alleles at the QTLs determining resistance to L. taurica.
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04133318/file/JJC%20AUSSOIS%202022%20Mycologie-FCussonneau-soumis.pdf BibTex
titre
A plant-specific homolog of DP1/Yop1 family proteins plays a proviral role in potyvirus infection
auteur
Mingshuo Xue, Luc Sofer, Vincent Simon, Roxane Lion, Nathalie Arvy, Jens Tilsner, Sylvie German-Retana, Mamoudou Diop, Jean-Luc Gallois
article
The 41st ASV Annual Meeting, Jul 2022, Madison (US-WI), United States
resume
The Potyvirus genus is one of the largest genera of plant RNA viruses responsible for serious diseases in vegetable and fruit crops worldwide [1]. As potyviruses have developed strategies to hijack the host secretory pathway and plasmodesmata (PD) for their transport, the goal of this study was to identify membrane and/or PD-proteins that interact with one of the viral proteins involved in the cell-to-cell movement of turnip mosaic virus (TuMV) the “second 6 kDa molecular-weight membrane anchoring protein” (6K2). The 6K2 induces the formation of endoplasmic reticulum (ER)-derived viral vesicles, important not only for replication but also for intra- and intercellular movement of TuMV [2]. In Arabidopsis thaliana, AtHVA22a (Hordeum vulgare abscisic acid responsive gene 22) belongs to a multigenic family of transmembrane proteins, homologous to DP1/Yop1 family proteins in yeast and interactors of reticulons, which are responsible for the constriction of ER tubules. The role of HVA22 gene family has been poorly studied in plants, even if its potential involvement in response to abiotic stresses was highlighted [4]. Recent proteomics analysis of PD fractions purified from Arabidopsis thaliana suspension cells showed that AtHVA22a is highly enriched in plasmodesmata proteome [3]. Here, using a Split-ubiquitin membrane Y2H assay we screened an Arabidopsis cDNA library and identified AtHVA22a as an interactor of the 6K2 protein of TuMV. We further confirmed this interaction in yeast and in planta using bimolecular fluorescence complementation (BiFC) and showed that the 6K2/AtHVA22a interaction occurs at the level of the viral replication complexes during TuMV infection and partially at PD levels. Furthermore, overexpression of AtHVA22a increases TuMV propagation in Nicotiana benthamiana while TuMV propagation is slowed down in AtHVA22a-CRISPR/Cas9 mutants. Altogether, our results indicate that AtHVA22a plays an agonistic effect on TuMV propagation. [1] doi:10.1099/jgv.0.000740. [2] doi:10.3389/fmicb.2013.00351. [3] doi:10.15252/embr.201847182. [4] DOI: 10.1023/a:1015593715144
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Poster communications
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https://hal.inrae.fr/hal-04648013/file/German-Retana-et-al-ASV-2022-poster.pdf BibTex
titre
Analyzing Genetic Control Of Traits In Ecophy-genetic Modelling
auteur
Omar Belatmane, Florent Bonneu, Daniel Gourion, M. Memmah, Bénédicte Quilot-Turion
article
Tersys Implanteus st Summer School, Jul 2022, Avignon, France
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04201581/file/Poster_Omar_Belatmane.pdf BibTex
titre
Let's find more resilient trees to reduce the use of phytosanitary products in orchards
auteur
Marie Serrie, Alain Blanc, Laurent Brun, Fréderic Gilles, Véronique Signoret, Sabrina Viret, Jean-Marc Audergon, Bénédicte Quilot-Turion, Morgane Roth
article
Tersys Implanteus 1st Summer School (TISS1), Jul 2022, Avignon, France
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04181863/file/Poster_TISS1_Serrie.M.pdf BibTex
titre
A high-throughput image analysis method for assessing pepper quantitative resistance to Cucumber mosaic virus
auteur
Judith Hirsch, Marion Szadkowski, Sylvain Piry, Christelle Lacroix, Louis McLeod, Benoît Moury, Véronique Lefebvre
article
15. International symposium of plant virus epidemiology, Jun 2022, Madrid, Spain. , 2022
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Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03689913/file/2022_Hirsch_IPVE.pdf BibTex
titre
CAP ZERO PHYTO: Developing the concept of agroecological immunity
auteur
Hélène Gautier, Bernard Caromel, Marie-Noëlle Brisset, Véronique Lefebvre
article
European Scientific Conference ‘Towards Pesticides Free Agriculture’, Jun 2022, Dijon, France
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Poster communications
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titre
Phenotyping peach orchards with increased accuracy and throughput: perspectives of digital phenotyping
auteur
Morgane Roth, Joss Gillet, Mathias Lefebvre, Alexis Comar, Fabien Miart, Adrien Vielix, Etienne David, Bénédicte Quilot-Turion, Véronique Signoret, Marie Serrie, Sabrina Viret, Raul Lopez Lozano, Sylvain Jay, Marie Weiss
article
X International Peach Symposium, May 2022, Nouassa, Greece
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Poster communications
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titre
Which phloem in Cucumis melo is exploited by Aphis gossypii ?
auteur
Pierre Sadon, Marie-Noëlle Corre, Pascale Mistral, Raphaël Lugan, Olivier Chevallier, Nathalie Boissot, Marie Noëlle Corre
article
Réunion scientifique plénière BAPOA, May 2022, Nice, France
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03657121/file/20220502%20-%20Poster%20BAPOA%20version%20finale%20%C3%A0%20imprimer.pdf BibTex

Reports

titre
État des connaissances sur la contribution des technologies d'édition du génome à l’amélioration des plantes pour la transition agroécologique et l'adaptation au changement climatique
auteur
Carole Caranta, Mathilde Causse, Fabien Nogué, Annabelle Déjardin, Emilie Gentilini, Philippe Debaeke, Laurent Hazard, Sophie Le Perchec, Stéphane Lemarié, Guy Richard, Peter Rogowsky, Marie-Violaine Tatry
article
INRAE. 2022
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Reports
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03943821/file/ETUDE%20EDITION%20du%20GENOME%20et%20AGROECOLOGIE_VF.pdf BibTex
titre
PROJET CASDAR : Caractérisation des Ressources Génétiques Prunus pour les stress Biotiques et Abiotiques
auteur
Bénédicte Quilot-Turion
article
inrae. 2022
resume
Le projet CaRessPrunus qui porte sur les Prunus cultivés (pêcher, cerisier et abricotier), adresse ces questions en les abordant sous l’angle génétique qui complète les approches aujourd’hui prise en compte dans les programmes Ecophyto Déphy et Fermes. Il vise la caractérisation des ressources génétiques disponibles pour les stress abiotiques (en lien avec les perspectives de changement climatique) et pour les stress biotiques (hors maladies de quarantaine déjà travaillées par ailleurs). Cette caractérisation ‘multi-traits’ des ressources génétiques s’inscrit dans une démarche à moyen terme de caractérisation des ressources disponibles et à long terme d’optimisation des programmes d’amélioration et de sélection des arbres fruitiers pour des écosystèmes résilients. Elle intègre aussi un enjeu du plan SPAD2 destiné à objectiver l’émergence d’une VATE chez les espèces fruitières. Le projet CaRessPrunus est structuré en 4 actions complémentaires (Figure O.1) destinées : -à identifier, avec les acteurs de la filière, les enjeux liés aux caractères ciblés (indicateurs, priorisation, méthodologie de caractérisation) – (Action 1),-à caractériser les traits liés aux stress abiotiques (Action 2), avec une cible prioritaire la phénologie (dormance, floraison, maturité, sénescence) comprenant tout d’abord une caractérisation de traits identifiés comme importants suite à l’action 1 et une analyse plus fine de la dormance déjà initiée sur un sous-ensemble du matériel étudié dans ce projet (notamment sur le cerisier comme espèce modèle), -à caractériser les traits liés aux stress biotiques (Action 3) (hors maladies de quarantaine travaillées par ailleurs), avec deux approches complémentaires : oune approche ciblée sur les traits précisément identifiés dans l’action 1, oet une approche plus intégratrice destinée à évaluer le comportement de matériels sous faibles intrants phytosanitaires afin d’appréhender les risques spécifiquement induits par la réduction drastique de la protection phytosanitaire dans le cadre de dispositifs expérimentaux mis en place dans le cadre du projet, -à profiter des données de marquage moléculaire disponibles pour identifier les déterminants génétiques et moléculaires des traits associés (Action 4).
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Reports
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04172261/file/Rapport%20final_CASDAR_CaRessPrunus_2022.02.21_BQT.pdf BibTex

Preprints, Working Papers, ...

titre
Mapping the intensity function of a non-stationary point process in unobserved areas
auteur
Edith Gabriel, Francisco J. Rodríguez-Cortes, Jérôme Coville, Jorge Mateu, Joël Chadoeuf
article
2022
resume
Seismic networks provide data that are used as basis both for public safety decisions and for scientific research. Their configuration affects the data completeness, which in turn, critically affects several seismological scientific targets (e.g., earthquake prediction, seismic hazard...). In this context, a key aspect is how to map earthquakes density in seismogenic areas from censored data or even in areas that are not covered by the network. We propose to predict the spatial distribution of earthquakes from the knowledge of presence locations and geological relationships, taking into account any interaction between records. Namely, in a more general setting, we aim to estimate the intensity function of a point process, conditional to its censored realization, as in geostatistics for continuous processes. We define a predictor as the best linear unbiased combination of the observed point pattern. We show that the weight function associated to the predictor is the solution of a Fredholm equation of second kind. Both the kernel and the source term of the Fredholm equation are related to the first-and second-order characteristics of the point process through the intensity and the pair correlation function. Results are presented and illustrated on simulated non-stationary point processes and real data for mapping Greek Hellenic seismicity in a region with unreliable and incomplete records.
typdoc
Preprints, Working Papers, ...
Accès au texte intégral et bibtex
https://hal.science/hal-03452637/file/Gabriel2022_Local_intensity.pdf BibTex

2021

Conference papers

titre
Construction and diversity of the G2P-SOL pepper core collection
auteur
Véronique Lefebvre
article
G2P- SOL FINAL WORKSHOP “Multi-omics-based management of plant genetic resources”, Dec 2021, on line, Italy
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Conference papers
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titre
Une structure emboîtée universelle pour les interactions quantitatives entre les plantes et leurs parasites ?
auteur
Benoît Moury, Jean-Marc Audergon, Sylvie Baudracco-Arnas, Safa Ben Krima, François Bertrand, Nathalie Boissot, Mireille Buisson, Valérie Caffier, Melissa Cantet, Sylvia Chanéac, Carole Constant, Francois F. Delmotte, Catherine Dogimont, Juliette Doumayrou, Frédéric Fabre, Sylvain Fournet, Valérie Grimault, Thierry Jaunet, Isabelle Justafré, Véronique Lefebvre, Denis Losdat, Thierry C. Marcel, Josselin Montarry, Cindy E. Morris, Mariem Omrani, Manon Paineau, Sophie Perrot, Marie-Laure Pilet-Nayel, Youna Ruellan
article
Réunion annuelle du réseau E3GP3, Dec 2021, Visioconférence, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03553078/file/Moury_HAL_INRAE.pdf BibTex
titre
Pepper core collection trials and GWAS
auteur
Véronique Lefebvre
article
The advanced Training school II “Using Genetic Resource Data for Breeding”, Oct 2021, on line, Israel
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Conference papers
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titre
Outside the wild: Risks and mechanisms of host jumps of endive necrotic mosaic potyvirus
auteur
Marion Szadkowski, Catherine Wipf-Scheibel, William Billaud, Cecile Desbiez, Jonathan Gaudin, Gregory Girardot, Patrick Gognalons, Judith Hirsch, Thibaud Jayet, Sylvain Piry, Loup Rimbaud, Alexandra Schoeny, Eric Verdin, Raphael Forien, Julien Papaïx, Lionel Roques, Benoît Moury, Karine Berthier
article
18. Rencontres de virologie végétale (RVV 2021), Cirad; INRAE, Sep 2021, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03368388/file/2021_Szadkowski_RVV.pdf BibTex
titre
Potato virus Y adaptation to various resistance QTL combinations in pepper and impact on host tolerance
auteur
Thibaud Jayet, William Billaud, Lucie Tamisier, Marion Szadkowski, Catherine Wipf-Scheibel, Loup Rimbaud, Judith Hirsch, Gregory Girardot, Jonathan Gaudin, Alexandra Schoeny, Karine Berthier, Karine Nozeran, Elise Lepage, Sarah de Colle-Guihéneux, Thomas Pressecq, Véronique Lefebvre, Benoît Moury
article
18. Rencontres de virologie végétale (RVV 2021), Cirad; Inrae, Sep 2021, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03368394/file/Jayet_HAL-INRAE.pdf BibTex
titre
Many different recipes but one flavor: A universal nested structure of quantitative interactions between plant genotypes and their parasites, including viruses
auteur
Benoît Moury, Jean-Marc Audergon, Sylvie Baudracco-Arnas, Safa Ben Krima, François Bertrand, Nathalie Boissot, Mireille Buisson, Valérie Caffier, Melissa Cantet, Sylvia Chanéac, Carole Constant, François Delmotte, Catherine Dogimont, Juliette Doumayrou, Frédéric Fabre, Sylvain Fournet, Valérie Grimault, Thierry Jaunet, Isabelle Justafré, Véronique Lefebvre, Denis Losdat, Thierry C. Marcel, Josselin Montarry, Cindy E. Morris, Mariem Omrani, Manon Paineau, Sophie Perrot, Marie-Laure Pilet-Nayel, Youna Ruellan
article
18. Rencontres de virologie végétale (RVV 2021), INRAE; CIRAD, Sep 2021, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03553180/file/Moury_HAL-INRAE.pdf BibTex
titre
Determinism of virulence of cucumber vein yellowing virus (CVYV) in melon and insights into the durability of resistance
auteur
Cecile Desbiez, Maria Luisa Domingo Calap, Michel Pitrat, Catherine Wipf-Scheibel, Gregory Girardot, Inmaculada Ferriol, Juan Jose Lopez-Moya, Hervé Lecoq
article
18. Rencontres de Virologie Végétale, Sep 2021, Aussois, France
resume
Genetic resistance is a sustainable way of controlling plant viruses, but it requires the presence of resistance genes in the host germplasm. Besides, its durability can be reduced by resistancebreaking isolates, either pre-existing in the environment or emerging after the deployment of the resistance. In the case of cucumber vein yellowing virus (CVYV, genus Ipomovirus), an emerging virus on cucurbits in the Mediterranean Basin, few resistances are available in melon. The accession PI 164323 was found to display complete resistance to the strain CVYV-Esp, and accession HSD 2458 presented a tolerance, i.e. very mild symptoms in spite of virus accumulation in inoculated plants. The resistance is controlled by one dominant allele Cvy-1 1 , while the tolerance is controlled by a recessive allele cvy-2 independent from Cvy-1 1 . Upon inoculation with eight molecularly diverse CVYV isolates, the resistance was found to be strain-specific since many CVYV isolates induced necrosis on PI 164323, whereas the tolerance presented a broader range. A resistance-breaking isolate inducing severe mosaics on PI 164323 was obtained. It differed from the parental strain by a single amino-acid change in the VPg-coding region. The effect on the mutation was confirmed by reverse genetics using a CVYV infectious clone. Competition experiments suggested a fitness cost of the resistancebreaking mutation in susceptible melon. Our results highlight the need to combine cultural practices and/or additional genes to develop a more durable control of CVYV than the use of the Cvy-1 1 allele alone.
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03555763/file/2021-Desbiez-RVV.pdf BibTex
titre
Experimental evolution of a plant RNA virus: a warning about spurious correlations when studying fitness changes
auteur
Lucie Tamisier, Frédéric Fabre, Marion Szadkowski, Ghislaine Nemouchi, Gregory Girardot, Pauline Millot, Alain Palloix, Benoît Moury
article
18. Rencontres de virologie végétale (RVV 2021), INRAE; CIRAD, Sep 2021, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03553147/file/Tamisier_HAL-INRAE.pdf BibTex
titre
Breeding tomato hybrids for flavour: comparison of GWAS on lines and F1 hybrids
auteur
Estelle Bineau, José Luis Rambla, Santiago Priego, Alexandre Hereil, Frédérique Bitton, Clémence Plissonneau, Antonio Granell, Mathilde Causse
article
Online EUCARPIA congress 2021 - Breeding: the key to innovative solutions, WUR, Aug 2021, Online, Netherlands
resume
While breeders face consumer expectations regarding tomato flavour in the F1 hybrid varieties commercialised, no data are available on the inheritance of QTLs for flavour-related trait in heterozygous plant material. Volatile organic compounds (VOCs) are major determinants of tomato lavour. We quantified 46 VOCs in (1) a panel of 121 small fruited tomato accessions (15 S. pimpinellifolium and 106 S. lycopersicum var. cerasiforme) and (2) in a test cross panel involving the previous panel plus 44 elite cherry tomato lines crossed with a common big fruited line. High and consistent heritabilities were assessed for most VOCs in the two panels, and the line and test cross values for VOC content were strongly correlated. In the two panels, cluster analysis on 46 VOC content matched the metabolic pathways known from the literature. Results suggested a global additivity pattern for most VOC genetic control. We performed genome wide association studies (GWAS) on the line and test cross panels separately, along with a third GWAS on the test cross subset carrying the subset of 121 F1 hybrids corresponding to the line panel. We identified 205, 183 and 138 associations, respectively. Although less than 10% associations overlapped between line and F1 hybrid panels, we identified numerous overlapping associations for VOCs belonging to the same metabolic pathway within each panel. With this study, we highlighted the interest of testcross panel to create tasty F1 hybrid varieties. The clusters of associations in the F1 hybrid panel are new levers to improve tomato flavour by regulating several key VOCs simultaneously, while following only a few genomic regions in the breeding material. We selected seven chromosome regions fitting numerous criteria meant to facilitate breeding. For instance, a 4Mbp region on chromosome 3 carried F1 specific associations for 14 different VOCs derived from the lipid metabolism, eight of which having an impact on tomato overall flavour. This region is supported by several candidate genes also highlighted in previous studies.
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03562573/file/Abstract.Eucarpia.E.Bineau.pdf BibTex
titre
Automated phenotyping provides insights into leaf angle changes in chile pepper response to stress.
auteur
Derek W Barchenger, Roland Schaftleitner, Ilan Paran, Véronique Lefebvre, Pasquale Tripodi, Nils Stein, Richard Finkers, Giovanni Giuliano, Seok-Beom Kang
article
ASHS 2021 Annual Conference, Aug 2021, Denver (Colorado), United States
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Conference papers
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https://hal.inrae.fr/hal-03551646/file/Barchenger%202021%20ASHS%20Denver%20-%20oral.pdf BibTex
titre
Recent advances on peach-brown rot interactions: searching solutions to fight in a sustainable manner
auteur
Bénédicte Quilot-Turion
article
ISHS Peach webinar Current trends and challenges on peach fruit production, Jun 2021, Cyprus, Cyprus. pp.1-21
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03445639/file/ISHS%20Peach%20Webinar%20series%20%28official%20brochure%29.pdf BibTex
titre
Recent advances on peach-brown rot interactions from INRAE's research program
auteur
Bénédicte Quilot-Turion
article
10th ISHS International Peach Symposium A series of 4 HortDialogues webinars on peach 'Current trends and challenges on peach fruit production', International Society for Horticultural Science, Jun 2021, Webinar, Greece
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Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03267904/file/Recent%20advances%20on%20peach-brown%20rot_%20BQuilotTurion.pdf BibTex
titre
Adaptation of GWAS models for plant resistance to viruses: from rediscovering major genes to highlighting of new complex traits in breeding germplasm.
auteur
Severine Monnot, Laurence Moreau, Tristan Mary-Huard, Mélissa Cantet, Nathalie Boissot
article
XIIe Eucarpia Meeting on Cucurbit Genetics and Breeding, May 2021, Almeria, Spain. pp.1-16
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titre
Une histoire de la sélection des espèces légumières en France.
auteur
Mathilde Causse, Michel Pitrat
article
Fruits et Légumes : quelles évolutions, Journées d'études AAF, AEHA et GGEDD, Mar 2021, Paris, France
resume
La sélection met à disposition des producteurs et des consommateurs des variétés qui correspondent aux attentes socio-économiques. Ces attentes varient en fonction du temps. Jusqu'à la fin du XVIII e siècle la société est très majoritairement rurale dans une économie de subsistance. Les légumes les plus consommés sont des légumes-racines (navets), des légumes-feuilles (choux, bettes, poireaux) et des légumes-graines (pois, lentilles, fèves). La sélection et la production de semences sont le fait des producteurs/consommateurs. Nous sommes mal renseignés sur les variétés mais elles sont assez nombreuses et correspondent à une adaptation pédo-climatique locale. Du début du XIX e au milieu du XX e siècle, une économie de marché se développe avec l'exode rural et le développement des transports qui vont entraîner d'une part une localisation des producteurs vers les ceintures vertes des villes et des régions spécialisées et d'autre part le développement des grainetiers avec l'achat des semences. Les variétés se diversifient avec en particulier des efforts d'homogénéisation et une adaptation au forçage sous châssis. Les légumes-fruits deviennent plus importantes (tomate, concombre, courgette). Depuis le milieu du XX e siècle, l'internationalisation des marchés, le développement de l'industrie agro-alimentaire (2 ème à 5 ème gamme), la part prise par la grande distribution dans le commerce des légumes, les évolutions des techniques de production (serres, mécanisation) entraînent le développement de nouvelles variétés répondant à ces demandes. Le secteur de l'innovation variétale et de la production de semences est très dynamique.
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03562674/file/resume3.pdf BibTex
titre
Résistance à Xanthomonas arboricola pv. pruni chez le pêcher
auteur
Naïma Dlalah, Bénédicte Quilot-Turion, Yannick Montrognon
article
Les Rencontre du GIS, GIS, Jan 2021, webinaire, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03269432/file/19-01-21_GIS%20fruits_ReXapPech.pdf BibTex

Master thesis

titre
Identification and characterization of phenotypic and genetic components of resilience in apricot and peach by using genome wide association studies
auteur
Marie Serrie
article
Biologie végétale. 2021
resume
To date, there are still no apricot or peach varieties that are resistant to most of the pests and diseases. The development of new resilient varieties to deal with this issue in the long term is one of the major challenges for the sector. However, the nature of Prunus resilience is still largely unknown. In order to progress in the understanding of this character, a monitoring of two core-collections composed by 150 apricot and 206 peach accessions managed under conditions of low phytosanitary protection and deployed respectively in two and three sites has been set up. More specifically, we observed the damages caused by eight different pests and diseases present in natural conditions as well as three phenological traits of interest. We observed a pronounced phenotypic diversity of these traits within the core-collections as well as very variable heritability values. Moreover, the contribution of the genetic part to the phenotypic variation was significant for most of these traits. We also identified a strong effect of the environment and of the genotype-environment interactions. In addition, thanks to a dense genotyping of the peach core-collection accessions, we were able to perform diversity analyses. Thus, we highlighted an important structuration of the peach core-collection in three large genetic groups. Furthermore, by using genome wide association analyses performed with the multi linear mixt model (MLMM), we detected four SNPs related to two key phenological stages - leaf and floral emergence - as well as two SNPs linked to the resistance of two major peach diseases : coryneum and oriental peach moth or leafminer. Finally, we identified three accessions with low symptoms for all of the pests and diseases studied. This identification of resilience factors and candidate genotypes represents an interesting opportunity for breeding resilience traits.
typdoc
Master thesis
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03880818/file/2021%20APIMET%20SERRIE.pdf BibTex

Other publications

titre
Large-scale geography survey provides insights into the colonization history of a major aphid pest on its cultivated apple host in Europe, North America and North Africa
auteur
S Olvera-Vazquez, C Remoué, A Venon, A Rousselet, O Grandcolas, M Azrine, L Momont, M Galan, L Benoit, G David, A Alhmedi, T Beliën, G Alins, P Franck, A Haddioui, S Jacobsen, R Andreev, S Simon, L Sigsgaard, E Guibert, L Tournant, F Gazel, K Mody, Y Khachtib, A Roman, T Ursu, I Zakharov, H Belcram, M Harry, M Roth, J Simon, S Oram, J Ricard, A Agnello, E Beers, J Engelman, I Balti, A Salhi-Hannachi, H Zhang, H Tu, C Mottet, B Barrès, A Degrave, J Razmjou, Tatiana E Giraud, M Falque, E Dapena, M Miñarro, L Jardillier, P Deschamps, E Jousselin, A Cornille
article
2021, pp.e34. ⟨10.24072/pci.evolbiol.100134⟩
resume
With frequent host shifts involving the colonization of new hosts across large geographical ranges, crop pests are good models for examining the mechanisms of rapid colonization. The microbial partners of pest insects may also be involved in or affected by colonization processes, which has been little studied so far. We investigated the demographic history of the rosy apple aphid, Dysaphis plantaginea, a major pest of the cultivated apple (Malus domestica) in Europe, North Africa and North America, as well as the diversity of its microbiota. We genotyped a comprehensive sample of 714 colonies from Europe, Morocco and the US using mitochondrial (CytB and CO1), bacterial (16s rRNA and TrnpB), and 30 microsatellite markers. We detected five populations spread across the US, Morocco, Western and Eastern Europe and Spain. Populations showed weak genetic differentiation and high genetic diversity, except the ones from Morocco and North America that are likely the result of recent colonization events. Coalescent-based inferences revealed high levels of gene flow among populations during the colonization but did not allow determining the sequence of colonization of Europe, North America and Morroco by D. plantaginea, likely because of the weak genetic differentiation and the occurrence of gene flow among populations. We found that D. plantaginea rarely hosts other endosymbiotic bacteria than its obligate nutritional symbiont Buchnera aphidicola. This suggests that secondary endosymbionts did not play an important role in the rapid spread of the rosy apple aphid. These findings have fundamental importance for understanding pest colonization processes and implications for sustainable pest control programs.
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Other publications
Accès au texte intégral et bibtex
https://hal.science/hal-03619354/file/Olvera-Vazquez_pcjournal_26.pdf BibTex
titre
Une lutte mieux armée face à "Xanthomonas
auteur
Bénédicte Quilot-Turion, Naïma Dlalah, Yannick Montrognon
article
2021, pp.30-31
resume
Dans le cadre d'un projet porté par INRAE d'Avignon et la Sefra, un test ADN a été développé pour cribler efficacement les variétés de pêchers vis à vis de la maladie provoquée par " Xanthomonas". Il représente une aide non négligeable à la sélection et à la caractérisation variétale.
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Other publications
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titre
Des composés de la pêche aux effets fongicides contre la moniliose
auteur
Marie-Noëlle Corre
article
2021
typdoc
Other publications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03298409/file/Corre_ComposesPecheMoniliose_ReussirF_L_2021.jpg BibTex

Poster communications

titre
Identification of volatile organic compounds emitted by the almond tree in view of developing a biocontrol tool for Eurytoma amygdali
auteur
Anjelica Leconte, Philippe P. Lucas, Bruno Buatois, Nicolas Barthes, Eugénie Coutagne, Henri Duval, Michael Staudt
article
Journées GDR MediatEC 2021, Oct 2021, Toulouse, France.
typdoc
Poster communications
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titre
EXploring PhenOtypic SpacE for mining genotypes and alleles in Maize
auteur
Emilie Millet, Claude Welcker, Llorenç Cabrera-Bosquet, Boris Parent, François Tardieu, Denis Vile, Cyrille Violle, Frédéric Chazale, Randall J Wisser
article
4. European Maize Meeting, Sep 2021, Regensburg, Germany
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04154374/file/Poster_MaizeMeeting_MilletE.pdf BibTex

Reports

titre
Projet Phytoscope CPER 2021-2027
auteur
Bénédicte Quilot-Turion
article
PACA P21, INRAE. 2021
resume
Plateforme collaborative d'observation et d'expérimentation pour l'horticulture, l'agro-écologie et la forêt méditerranéenne Site porteur du projet INRA Centre PACA-site d'Avignon (en 2020 INRAE
typdoc
Reports
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04172827/file/Phytoscope%20Descriptif%20scientifique%20V2021.pdf BibTex
titre
Résistance à Xanthimonas arboricula pv.pruni chez le pêcher
auteur
Bénédicte Quilot-Turion
article
AAP SSV 2018 n° C-2018-06, Inrae. 2021
resume
Le projet est organisé en 2 actions. La première action visait à valider un jeu de marqueurs moléculaires associés à la résistance et à la sensibilité à X. pruni dans des fonds génétiques différents de ceux jusque-là testés. Ainsi, un lot d’une quarantaine de variétés connues pour leur moindre ou forte sensibilité à X. pruni a été utilisé pour valider ces marqueurs. Ce test ADN peut être appliqué en routine à des variétés ou descendances en ségrégation pour les caractériser/les cribler vis-à-vis de cette maladie bactérienne. L’objectif est de pouvoir cribler efficacement les variétés de pêchers vis-à-vis de cette maladie et pouvoir ainsi les recommander à la plantation en zones sensibles. Un panel de 102 variétés et hybrides appartenant à des programmes de sélection d’obtenteurs privés et 191 accessions de la collection pêcher INRAE a été caractérisé avec le test ADN. Cela a permis de faire progresser les connaissances quant à la variabilité génétique disponible chez le Pêcher vis-à-vis de X. pruni. Un calendrier des variétés conseillées pour la plantation en zone de forte pression de la maladie a été élaboré à partir de ces résultats (Calendrier Variétés 2020). Un tel outil d’aide à la décision s’adresse potentiellement à différents acteurs de la filière Pêcher : - les sélectionneurs-obtenteurs-éditeurs de variétés de pêcher publiques et privés (aide à la sélection), - les différentes structures publiques ou interprofessionnelles ayant en charge la caractérisation variétale : - les responsables techniciens de développement en charge de la caractérisation variétale organisée dans le cadre de la Charte Fruitière (Ctifl, stations régionales d’expérimentation : vergers de comportement de niveau 1 et 2) - les instances nationales ou communautaires en charge des examens de DHS pêcher (INRA-GEVES-CTPS/INOV/OCVV) pour l’inscription et la protection des nouvelles variétés. In fine, l’information recueillie bénéficie concrètement aux arboriculteurs soucieux de planter des variétés de pêches et de nectarines “résistantes” en zones sensibles à X. pruni. La seconde action avait pour objectif la création de matériel végétal Pêcher résistant à X. pruni, à partir de pro-géniteurs de résistance à cette maladie fournis par une équipe américaine de l’Université de Clemson (USA). Les individus hybrides issus de deux croisements ont été plantés dans une parcelle de verger de la SEFRA (Rhône-Alpes), dans laquelle cette maladie bactérienne est endémique. A partir de ces descendances, des croisements ont été réalisés pour obtenir des individus homozygotes résistants aux 2 loci, qui serviront de géniteurs aux sélectionneurs privés pour développer des gammes variétales complètes de pêches et de nectarines résistantes à la maladie. En outre, ce projet a permis de renforcer les partenariats entre la recherche publique (INRA), les instituts techniques (Ctifl), les stations régionales d’expérimentation (SEFRA) et les organisations professionnelles (AOP Pêches et Abricot de France). Les résultats ont été présentés à de nombreuses réunions (Topo Rencontres du GIS Fruits 2021). Le projet RéXapPêch est déjà notoirement cité dans la filière comme un exemple de projet collaboratif à vocation appliquée réussi et exemplaire.
typdoc
Reports
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04172256/file/Synthese_ReXaPech_2021.pdf BibTex

Preprints, Working Papers, ...

titre
The quasi-universality of nestedness in the structure of quantitative plant-parasite interactions
auteur
Benoît Moury, Jean-Marc Audergon, Sylvie Baudracco-Arnas, Safa Ben Krima, François Bertrand, Nathalie Boissot, Mireille Buisson, Valérie Caffier, Melissa Cantet, Sylvia Chanéac, Carole Constant, François Delmotte, Catherine Dogimont, Juliette Doumayrou, Frédéric Fabre, Sylvain Fournet, Valérie Grimault, Thierry Jaunet, Isabelle Justafré, Véronique Lefebvre, Denis Losdat, Thierry Marcel, Josselin Montarry, Cindy E. Morris, Mariem Omrani, Manon Paineau, Sophie Perrot, Marie-Laure Pilet-Nayel, Youna Ruellan
article
2021
resume
Understanding the relationships between host range and pathogenicity for parasites, and between the efficiency and scope of immunity for hosts are essential to implement efficient disease control strategies. In the case of plant parasites, most studies have focused on describing qualitative interactions and a variety of genetic and evolutionary models has been proposed in this context. Although plant quantitative resistance benefits from advantages in terms of durability, we presently lack models that account for quantitative interactions between plants and their parasites and the evolution of these interactions. Nestedness and modularity are important features to unravel the overall structure of host-parasite interaction matrices. Here, we analysed these two features on 32 matrices of quantitative pathogenicity trait data gathered from 15 plant-parasite pathosystems consisting of either annual or perennial plants along with fungi or oomycetes, bacteria, nematodes, insects and viruses. The performance of several nestedness and modularity algorithms was evaluated through a simulation approach, which helped interpretation of the results. We observed significant modularity in only six of the 32 matrices, with two or three modules detected. For three of these matrices, modules could be related to resistance quantitative trait loci present in the host. In contrast, we found high and significant nestedness in 30 of the 32 matrices. Nestedness was linked to other properties of plant-parasite interactions. First, pathogenicity trait values were explained in majority by a parasite strain effect and a plant accession effect, with no parasite-plant interaction term. Second, correlations between the efficiency and scope of the resistance of plant genotypes, and between the host range breadth and pathogenicity level of parasite strains were overall positive. This latter result questions the efficiency of strategies based on the deployment of several genetically-differentiated cultivars of a given crop species in the case of quantitative plant immunity.
typdoc
Preprints, Working Papers, ...
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03169796/file/2021_Moury_bioRxiv.pdf BibTex

2020

Conference papers

titre
Wounding nectarine fruit disrupts Monilinia laxa infection: deciphering fruit gene pathway involved and the role of phenolic and volatile compounds
auteur
Bénédicte Quilot-Turion, Marie-Noëlle Corre, Guy Costagliola, Laure Heurtevin, Véronique Signoret, Maria Do Carmo Raseira, Maximiliano Dini Vinoly
article
10th Rosaceae Genomics Conference, Centre for research in agricultural genomics, Dec 2020, Barcelone, Spain
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03267785/file/RGC10_QuilotTurion.pdf BibTex
titre
A transposable element from the hAT-superfamily is responsible for the absence or globose-shape phenotype of leaf extrafloral nectaries in peach (Prunus persica L. Batsch)
auteur
P Lambert, C Confolent, N Dlalah, Bénédicte Quilot-Turion, V Signoret, T Pascal
article
10th Rosaceae Genomics Conference, centre for research in agricultural genomics, Dec 2020, Barcelona, Spain
resume
Most peach (Prunus persica L. Batsch) cultivars have extrafloral nectaries (EFNs), or leaf-glands, on the leaf petioles, stipules, or margins (Gregory 1915, Okie 1998). From an extensive study, Gregory (1915) observed that the great majority of varieties was found to have well-defined gland shapes. Moreover gland-shapes were generally homogenous on typical shoots, although some cultivars could exhibit mixed glands. This author identified three main types of leaves, those with reniform glands, those with globose glands and glandless leaves. Fruit breeding programs have inadvertently produced peach cultivars with glandless leaves (Okie 1998), yet without determining the effects on either natural enemies or herbivorous pests (Scorza and Sherman 1996). Empirical observations, however, showed that the absence of EFNs resulted in a higher susceptibility to peach powdery mildew (PPM), a major disease of the peach caused by Podosphaera pannosa var. persicae (Weinhold 1961; Monet 1983). As a result, peach seedlings without leaf-glands were systematically discarded in the breeding programs. The Mendelian inheritance of the leaf-gland phenotype (E/e) was first described by Connors (1921) and the E locus was latter mapped on chromosome 7 of the peach (Dettori et al., 2001), but without identifying the genetic factor involved. In order to address the latter point and implement early diagnosis tools, we developed a mapping population of 833 progenies derived from the selfing of 'Malo Konare', a canning peach cultivar with globose leaf-glands, from Bulgarian origin. This population was used to map and investigate the genetic factor underlying the E locus, using additional resources including NGS peach resources and 148 cultivars from various origins. Our findings demonstrated that a MITE-like Moshan transposable element inserted in the gene controlling the character was responsible for the absence or globose-shape phenotype of the EFNs.
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03267833/file/Abstract_lambert_RGC10.pdf BibTex
titre
Genebank genomics highlights novel centres of diversity and routes of migration in pepper (Capsicum spp.)
auteur
Pasquale Tripodi, Lorenzo Barchi, S Kale, S Esposito, M T Rabanus-Wallace, A Acquadro, R Schafleitner, J Prohens, M J Diez, A Börner, A Bovy, Jérémy Salinier, F Boyaci, G Pasev, R Brandt, Axel Himmelbach, E Portis, R Finkers, S Lanteri, I Paran, Véronique Lefebvre, G Giuliano, N Stein
article
SOL International Meeting 2020, Nov 2020, on line, United States
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03551675/file/Tripodi%202020%20SOL%20genomics%20on%20line%20-%20%20oral%20.pdf BibTex
titre
Identification de composés naturels de la pêche affectant la pathogénicité de Monilinia Spp.
auteur
Marie-Noëlle Corre
article
Rencontres du GIS Fruits, GIS, Oct 2020, Webinaire, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03269430/file/GIS%20Fruits%20MNCorre_28102020.pdf BibTex
titre
eIF4E-based resistance to potyviruses from natural variation to edited genes
auteur
Jean-Luc Gallois
article
International workshop on plant genome editing, Sep 2020, conférence en ligne, France
typdoc
Conference papers
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titre
Deciphering the epigenetic variability in S. lycopersicum accessions and their influence on hybrid vigor
auteur
Luisa Bermudez, Estanislao Burgos, Laura Pascual, Mathilde Causse, Magdalena Rossi, Fernando Carrari
article
SOlanaceae Genomics, Sep 2020, Online, Italy
resume
Heterosis is a fundamental biological phenomenon defined by the superior performance of hybrids over its parents in many economically important traits. Even though heterosis has been extensively used in agriculture, both in plants and animals, the underlying molecular basis remains elusive. This lack of knowledge hampers the rational design of hybrids in order to obtain, in a faster and more efficient way, benefits resulting from heterotic effects. Evidence obtained from several species allows hypothesizing that alterations in the epigenetic state, mediated by changes in the DNA methylation pattern, play a role in determining hybrid vigor. Nevertheless, how parental alleles interact in their progeny to influence the epigenome is poorly understood. We analyzed DNA methylomes, siRNAomes and transcriptomes of three tomato hybrids, and their corresponding divergent parents. Interestingly, the parental methylation, siRNAs and transcriptome patterns did not correlate with the genetic distance between the genotypes and several genes involved in key metabolic categories showed to be epigenetically regulated. Evenmore, hybrids displayed non-additive DNA methylation levels throughout the genome. These results add to the growing evidences that point epigenetic mechanisms as key determinants of hybrid performance and make them able to future applications in breeding programs.
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Conference papers
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titre
Meta-analysis of GWAS provides new insights into genetic control of tomato flavor
auteur
Jiantao Zhao, Christopher Sauvage, Frédérique Bitton, Mathilde Causse
article
Plant Genome and genomics Conference, Mar 2020, Rotterdam, Netherlands
resume
Tomato flavor has changed over the course of long-term domestication and intensive breeding. To understand the genetic control of flavor related traits, we performed a meta-analysis of genome-wide association studies (GWAS) for 18 traits, using 775 tomato accessions and 2,316,117 SNPs from three GWAS panels (Sauvage et al., 2014; Bauchet et al., 2017 and Tieman et al., 2017). In a first step, we imputed SNP data to increase genome coverage for two out of three panels and ran EMMAX software for each panel separately. From each run, the genomic inflation factor and standard errors of the beta coefficients were implemented in two meta-GWAS models: the inverse variance-weighted fixed effect and the Han Eskin random-effect model that takes heterogeneity across studies into account. Then, from the meta-GWAS results, we conducted a statistical fine-mapping of the candidate loci following a heuristic linkage disequilibrium (LD) approach. We discovered 305 significant associations for the contents of sugars, acids, amino acids and flavor-related volatiles. We showed that fruit citrate and malate contents have been impacted by selection during domestication and improvement, while sugar content has undergone less stringent selection. Results suggest that it may be possible to significantly increase volatiles that positively contribute to consumer preferences while reducing unpleasant volatiles, by selection of the relevant allele combinations. Our results provide genetic insights into the influence of human selection on tomato flavor and demonstrate the benefits obtained from meta-analysis.
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03562688/file/Meta_Rotterdam2020.pdf BibTex
titre
Reducing a model of sugar metabolism in peach fruit to explore genetic diversity
auteur
Hussein Kanso, Bénédicte Quilot-Turion, Mohamed-Mahmoud Memah, Olivier Bernard, Jean-Luc Gouzé, Valentina Baldazzi
article
iCROPM 2020 : Crop modelling for the Future, Feb 2020, Montpellier, France. pp.1-5
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03587179/file/ICROPM2020.pdf BibTex
titre
Meta-analysis of GWAS provides new insights into genetic control of tomato flavor.
auteur
Jiantao Zhao, Christopher Sauvage, Frédérique Bitton, Mathilde Causse
article
Plant and Animal Genome 2020, 2020, San Diego, United States
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03542371/file/Meta_PAG2020.pdf BibTex

Master thesis

titre
Chlorogenic acid impact on Monilinia Fructicola liquid culture and cutinase production
auteur
Arthur Lemozy
article
Phytopathology and phytopharmacy. 2020
resume
Monilinia fructicola, a pathogenic fungus of prunus genus, is responsible for brown rot disease, one of the most important disease regarding fruit economy. This study tries to find durable way to fight against this disease by exploiting natural phenolic compounds of peach flesh. We observed the effect of chlorogenic acid (ACQ) on M. fructicola pathogenic capacities by adding it to a liquid culture. Fungal biomass, medium protein content and ACQ stability in the medium were explored and proteomic profiles and targeted gene expression analyses were conducted. ACQ resulted in an increase of the mycelium biomass production as well as of the protein amount. The abundance of proteins secreted by the fungus in the medium was diversely impacted by ACQ. Cutinase abundance was reduced and RT-qPCR results showed a reduction of cutinase gene expression. The expressions of three polygalacturonase genes were also decreased. We observed medium acidification along the culture and demonstrated by HPLC analysis that M. fructicola induced ACQ degradation. The fungus probably used ACQ derivates as carbon source. These observations indicate that ACQ or its derivates can modify pathogenetic factors by modulating gene expression resulting in a modification of enzyme secretion.
typdoc
Master thesis
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-04197201/file/Rapport%20de%20stage_ArthurLemozy.pdf BibTex

Poster communications

titre
Small deletion in a photosynthesis-related gene is involved in anthocyanin accumulation in the mesocarp of bf blood-flesh peaches
auteur
Laure Heurtevin, Carole Confolent, Bénédicte Quilot-Turion, Sylvie Bureau, Carine Le Bourvellec, Zhijun Shen, Jacques Lagnel, Thierry Pascal
article
10th Rosaceae Genomics Conference, Dec 2020, Barcelone, Spain
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03267672/file/C0192.pdf BibTex
titre
Research of candidate genes in peach for resistance to root-knot nematodes and resistance to aphids Myzus persicae
auteur
Henri Duval, Laure Heurtevin, Naïma Dlalah, Jacques Lagnel, Caroline Callot
article
10th Rosaceae Genomics Conference, Dec 2020, Barcelona, Spain
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03267708/file/DUVAL_etal.pdf BibTex
titre
Apricot processing suitability linked to its macronutrient composition: a part of DeshyFruit project
auteur
Barbara Gouble, Patrice Reling, Adrien Servent, Pierre Brat, Véronique Vidal, Sandrine Laurent, Jean-Marc Audergon
article
Third Symposium on Fruit and Vegetable Processing, Nov 2020, Avignon, France. 1p
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03600777/file/P1-11_GOUBLE_Barbara_FVP2020.pdf BibTex
titre
Development of a Snakemake “framework/templates” to study the whole-genome transcriptional profiling from a blood-flesh trait (bf) and non blood-flesh trait (non-bf) cultivars in Prunus persica
auteur
Laure Heurtevin, Thierry Pascal, Bénédicte Quilot-Turion, Martin-Magniette Marie-Laure, Jacques Lagnel
article
JOBIM 2020 Journées Ouvertes de Biologie, Informatique et Mathématique, Jun 2020, Montpellier, France. ⟨10.13140/RG.2.2.15060.63366⟩
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-03267808/file/jobim2020_poster_188.pdf BibTex
titre
A decade of studies in France to decipher the genetic/molecular basis of eggplant resistance to bacterial wilt
auteur
Cyril Jourda, Sylvia Salgon, Arry Morel, Jérémy Guinard, Noura Yahiaoui, Marie-Christine Daunay, Emmanuel Wicker, Stéphane Poussier, Nemo Peeters, Jacques Dintinger
article
Rencontres Plantes-Bactéries, Jan 2020, Aussois, France. SFP, 2020
typdoc
Poster communications
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BibTex
titre
Hybrid de novo genome assembly using Oxford Nanopore Technology, 10X Genomics Linked-Reads sequencing, and Bionano optical map of a non-model species: the case of the melon-cotton aphid Aphis gossypii
auteur
Jacques Lagnel, Rafael Feriche-Linares, Pierre Bertrand, William Marande, Anne Loiseau, Amalia Sayeh, Maxime Manno, Nathalie Boissot
article
JOBIM, 2020, En ligne, France
resume
Insect species challenge high quality genome production because of their small size, the combination of high polymorphism and heterozygosity, the presence of repeat regions, and the pooling of polymorphic individuals to form libraries. No highly resolved genome is available for aphids which are major pests of cultivated plants. Aphis gossypii genome is expected with 339 Mbp in 4 pairs of chromosomes. To minimize heterozygosity, we focused on a lineage, C9, with a low estimated heterozygosity, and we pooled individuals deriving from clonal reproduction. We produced a high quality genome from 10X Genomics Linked-Reads (10X Genomics), long reads (Oxford Nanopore Technology: ONT), and Optical Map (Bionano), in order to compare advantages of combining different methods. We also produced a genome of a more heterozygous clone, NM1, but without an optical map support.
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Reports

titre
Séminaire d’échanges sur les pratiques de recherches interdisciplinaires à INRAE, 7-8 Janvier 2020, Synthèse
auteur
Isabelle Arpin, Olivier Barreteau, Carole Caranta, Christian Ducrot, Patrice Garin, Mourad Hannachi, Isabelle Maillet
article
[0] INRAE. 2020, 22 pp
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titre
Final resume on the research on the research unit Laboratory of Plant Ecophysiological responses to Environmental Stresses (LEPSE) under the supervision of the following institutions and research bodies, Montpellier SupAgro - Institut "Agro", Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement - INRAE
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Nelly Dupin, Erwin Dreyer, Sebastien Carpentier, Mathilde Causse, Sylvain Delzon, Christian Meyer, Thierry Ameglio, Philippe Hintzinger, Peter Rogowsky, Marie-Stephane Tixier
article
HCERES. 2020
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Document de création de l'UE A2M
auteur
Bénédicte Quilot-Turion
article
INRAe. 2020
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Preprints, Working Papers, ...

titre
The genomic basis of colour pattern polymorphism in the harlequin ladybird
auteur
Mathieu Gautier, Junichi Yamaguchi, Julien Foucaud, Anne Loiseau, Aurélien Ausset, Benoit Facon, Bernhard Gschloessl, Jacques Lagnel, Etienne Loire, Hugues Parrinello, Dany Severac, Celine Lopez-Roques, Cecile Donnadieu, Maxime Manno, Helene Berges, Karim Gharbi, Lori Lawson-Handley, Lian-Sheng Zang, Heiko Vogel, Arnaud A Estoup, Benjamin Prud'Homme Ben
article
2020
resume
Many animal species are comprised of discrete phenotypic forms. Understanding the genetic mechanisms generating and maintaining such phenotypic variation within species is essential to comprehending morphological diversity. A common and conspicuous example of discrete phenotypic variation in natural populations of insects is the occurrence of different colour patterns, which has motivated a rich body of ecological and genetic research. The occurrence of dark, i.e. melanic, forms, displaying discrete colour patterns, is found across multiple taxa, but the underlying genomic basis remains poorly characterized. In numerous ladybird species (Coccinellidae), the spatial arrangement of black and orange patches on adult elytra varies wildly within species, forming strikingly different complex colour patterns. In the harlequin ladybird Harmonia axyridis, more than 200 distinct colour forms have been described, which classic genetic studies suggest result from allelic variation at a single, unknown, locus. Here, we combined whole-genome sequencing, population genomics, gene expression and functional analyses, to establish that the gene pannier controls melanic pattern polymorphism in H. axyridis. We show that pannier, which encodes an evolutionary conserved transcription factor, is necessary for the formation of melanic elements on the elytra. Allelic variation in pannier leads to protein expression in distinct domains on the elytra, and thus determines the distinct colour patterns in H. axyridis. Recombination between pannier alleles may be reduced by a highly divergent sequence of ca. 170 kb in the cis-regulatory regions of pannier with a 50 kb inversion between colour forms. This likely helps maintaining the distinct alleles found in natural populations. Thus we propose that highly variable discrete colour forms can arise in natural populations through cis-regulatory allelic variation of a single gene.
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Preprints, Working Papers, ...
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2019

Conference papers

titre
Resistance to aphids in melon: R-cluster and QTLs
auteur
Nathalie Boissot
article
Plant-Insect-Symbiont interaction meeting, 2nd annual meeting of Japan-France PISI-net, Nov 2019, Le Rheu, France
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titre
Resistance to aphids in melon: R-cluster and QTLs
auteur
Nathalie Boissot
article
Plant-Insect-Symbiont interaction meeting, 2nd annual meeting of Japan-France PISI-net, Nov 2019, Le Rheu, France
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Conference papers
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titre
Within-plant genetic drift to control virus adaptation
auteur
Lucie Tamisier, Ghislaine Nemouchi, Marion Szadkowski, Gregory Girardot, Frédéric Fabre, Alain Palloix, Benoît Moury
article
5. Conférence Jacques Monod, Évolution virale sur le continuum mutualiste – parasite, Oct 2019, Roscoff, France
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titre
Résistance aux virus: Quand la variabilité naturelle guide l'édition de gènes
auteur
Jean-Luc Gallois
article
AFBV 9ème Colloquedes Biotechnologies Végétales, Gœthe Institut. Paris, FRA., Oct 2019, Paris, France
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A reduction strategy to simplify a model of sugar metabolism for application to a large panel of genotypes
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Hussein Kanso, Bénédicte Quilot-Turion, Mohamed-Mahmoud Memah, Olivier Bernard, Jean-Luc Gouzé, Valentina Baldazzi
article
FOSBE 2019 - 8th Conference on Foundations of Systems Biology in Engineering, IFAC, Oct 2019, Valencia, Spain
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titre
Dissecting G×E and QTL×E of maize yield in contrasting scenarios of light, heat and water deficit
auteur
Emilie J. Millet, Willem Kruijer, Santiago Alvarez Prado, Llorenç Cabrera-Bosquet, Aude Coupel-Ledru, Sébastien Lacube, Yves Gibon, Sylvain Prigent, Claude Welcker, Alain Charcosset, Fred A. van Eeuwijk, François Tardieu
article
XXIVth Eucarpia Maize and Sorghum Conference, TUM School of Life Sciences Weihenstephan, Oct 2019, Freising, Germany
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titre
Diversification : de nouveaux traits, de nouvelles espèces pour de nouveaux services et une stabilité des rendements en conditions de faibles intrants
auteur
Rémy Cailliatte, Jerome Enjalbert, Mireille Morisson, Delphine Moreau, Isabelle Litrico-Chiarelli
article
Journées Scientifiques du Département de Biologie et Amélioration des Plantes INRA, Sep 2019, Presqu'île de Giens, France
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Impact of host partial resistance on the gene expression of Phytophthora capsici: consequences for pepper breeding
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Véronique Lefebvre, Gaëtan Maillot, Emmanuel Szadkowski, Bernard Caromel, Anne Massire, Alexandre Bachellez, Nasradin Touhami, Guillem Rigaill, Joel Chadoeuf, Kurt Lamour
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant (2019), Sep 2019, Avignon, France
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Capsicum spp. and eggplant genome sequencing and resequencing provide new tools for the characterization of genetic resources
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L Barchi, Acquadro A, E. Portis, Alonso D, P. Gramazio, Vilanova S, M.J. Diez, Jérémy Salinier, A Unlu, L Toppino, Bassolino L, P Tripodi, D’agostino N, Börner A, Brandt R, D. Scaglione, Dicenta E, S Pinosio, G Aprea, Ferrante P, Finkers R, Boyaci Hf, A Bovy, N Stein, Schafleitner R, M.C. Daunay, Véronique Lefebvre, Lanteri S, J. Prohens, G.L. Rotino, Giuliano Sciara
article
17th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France
resume
BACKGROUND We report the current state of the art on genome sequencing and resequencing studies in Capsicum annuum L. and Solanum melongena as well as some of their cultivated and wild related species. The availability of high quality genome sequences in both species has opened the way to the characterization of genetic resources through genotyping-by-sequencing (GBS) approaches. Among them, we focus on the recently developed Single Primer Enrichment Technology (SPET) technique, which we have applied for targeted sequencing of a wide collection of S. melongena accessions and representatives of its primary, secondary and tertiary genepools. RESULTS Pepper Since the beginning of 2014, various consortia have released the genome sequences of domesticated and wild Capsicum species. The whole-genome sequences of C. annuum CM334 and C. chinense PI159236, which carry important disease resistance traits and have been widely used as parental lines of mapping populations, were the first to be released [1]. Next, the genome sequences of C. annuum Zunla-1 and of the wild species Chiltepin (C. annuum var. glabriusculum) were published [2]. Both studies highlighted that the pepper genome size is ~3-3.5 Gb, has a high percentage (over 80%) of repetitive elements, and encodes about 35K genes. Later, the improved version of the reference genome of CM334 and C. chinense PI159236, together with the sequencing of the domesticated C. baccatum were also made available, and contributed to deciphering the evolutionary relationships among the three species as well as to estimating the lineage-divergence times occurring in Capsicum [3]. By adopting the linked-read sequencing technology, the genome sequence of an F1 hybrid obtained by crossing CM334 with a nonpungent C. annuum breeding line was also published [4]. The availability of pepper genome sequences has allowed the resequencing of Capsicum accessions at both targeted genomic regions and the whole genome level. By applying a bulk segregant analysis, it has been possible to identify markers tightly linked to the Pvr4 locus, conferring dominant resistance to three pathotypes of Potyvirus (PVY, [5]). Furthermore, after the resequencing of resistant and susceptible cultivars, SNPs and putative alleles related to resistance against powdery mildew and bacterial wilt have also been detected [6,7]. To provide insights into the process of pepper domestication, the Zunla-1 genome sequence was analysed together with resequencing (20-30X) information of 18 cultivated accessions representative of the major varieties of C. annuum and two semi-wild/wild peppers [2]. This resulted in the identification of 115 genomic regions affected by artificial selection in cultivated peppers. Lastly, genome resequencing (30X) of four genotypes, representative of the main varietal types grown in the Mediterranean region, has been performed. Distinctive variations in miRNAs and resistance gene analogues (RGAs) have been highlighted as well as mutations in the coding sequences and regulatory regions of genes affecting fruit size and shape (Barchi et al., SOLCUC meeting 2017). Within the G2P-SOL EU project (www.g2p-sol.eu), the analysis of genome-wide genotyping-by-sequencing (GBS) data on 9,659 pepper accessions retrieved from the major European (CGN, INRA, IPK, UPV) and Asian (AVRDC) gene banks, Universities and Research Centres has been recently performed and the results obtained reported by Tripodi et al. (see Proceedings of this Meeting). Eggplant: In 2014, Hirakawa and co-authors [8] produced the first unanchored draft of the S. melongena genome sequence using the Nakate-Shinkuro accession. The obtained sequence covered about 70% of its projected 1.2 Gb genome size and more than 42K genes were identified. More recently, the Italian Eggplant Genome Consortium (IEGC) [9] developed a high quality and anchored genome assembly of the eggplant line 67/3, the male parent of an F6 RIL (Recombinant Inbred Line) mapping population. A hybrid assembly, covering 1.22 Gb, was obtained by merging Illumina sequencing data and optical mapping. The female parent of the RIL mapping population (line ‘305E40’) was also sequenced (coverage of 34X), and thanks to low coverage resequencing (1X) of the F6 RILs, the genome assembly was anchored to the 12 chromosomes. Recently, based on Illumina sequencing data, a draft genome assembly of 1.02 Gb in size was developed for the cultivated species S. aethiopicum, which contained as for eggplant, about 76% of repetitive sequences. Furthermore, compared to S. melongena, an expansion of gene families involved in drought or salinity tolerance as well as disease resistance including defence responses was identified. Recently, the resequencing of seven eggplant accessions and one accession of the wild relative S. incanum, which are the parents of a MAGIC population, has been performed (Gramazio et al., see Proceedings of this Meeting). The set of identified SNP polymorphisms has been annotated and currently is being used for further analyses in order to efficiently genotype the MAGIC population with the goal of dissecting key agronomic and morphological traits. More recently, 60 genotypes of the cultivated scarlet eggplant (S. aethiopicum) belonging to the varietal groups, “Gilo” and “Shum”, as well as 5 accessions of its ancestor S. anguivi, were sequenced at a coverage of 30-60X, with the goal of investigating the evolution, population demography and domestication history of the species [10]. Population structure of eggplant germplasm based on Single Primer Enrichment Technology (SPET) genotyping : Within the G2P-SOL EU project (www.g2p-sol.eu), which brings together the major European (CGN, INRA, IPK, UPV) and Asian (AVRDC) gene banks, Universities and Research Centres, 2,912 S. melongena, 305 S. aethiopicum and 122 S. macrocarpon accessions as well as a set of 266 accessions belonging to 29 wild species have been inventoried. The Single Primer Enrichment Technology (SPET) genotyping, recently developed by Nugen® was applied for their targeted genotyping. Starting from more than 12K polymorphic sites found in both coding regions and in the introns/UTRs, a set of 5K best performing SPET probes was used for diversity analyses, and a panel of about 25K high confident (min mean read depth of 30 and max missing data of 0.5%) SNPs evenly distributed throughout the genome were detected. The FastSTRUCTURE analysis (Fig. 1) identified 9 main clusters (K), with S. melongena accessions grouping into 6 sub-clusters. Three further clusters were found, including accessions belonging to S. macrocarpon and S. aethiopicum as well as species belonging to the subgenus Solanum, in good agreement with clustering obtained with a maximum likelihood phylogenetic tree. A total of 1,114 accessions were finally classified as admixed. The first two components of the PCA (Fig. 2) explained about 26% of the genetic variation. The first axis, explaining 15% of the genetic variation separated S. macrocarpon from the other species. The second component grouped separately S. melongena and S. aethiopicum as well as species from the Leptostemonum and Solanum subgenera. Several accessions had an unclear assignment, presumably due to misclassifications in the genebanks or gene flow among species. The gathered information was used for the development of a core collection of genotypes for future Genome Wide Association (GWA) studies. For this purpose, 15 S. melongena accessions and 5 wild species will be re-sequenced at 30X with the goal of identifying new SNPs useful for high resolution SPET genotyping.
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Unlocking genomic diversity of pepper (Capsicum spp.) collections held in genebanks: perspectives for breeding and germplasm management
auteur
Tripodi P, L Barchi, Acquadro A, Schafleitner R, J. Prohens, M.J. Diez, Börner A, Bovy As, Jérémy Salinier, Boyaci Hf, Pasev G, Brouwer M, Wallace T, Kale S, Esposito S, Brandt R, E. Portis, Finkers R, Lanteri S, Paran I, Véronique Lefebvre, Giuliano G, Stein N
article
. 17th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France. pp.1-3
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BACKGROUND The genetic diversity present in cultivars, landraces and wild relatives of modern crops, represents the source of agriculturally important genes to be discovered in our attempts of making crops more resilient and better adapted to the challenges of modern agriculture. In this context, genebanks are pivotal for the collection, preservation and distribution of germplasm; however, better knowledge of the available genetic variability would help improve the management of plant genetic resources. Here we report the analysis of genome-wide genotyping-by-sequencing (GBS) data on 9,659 pepper accessions retrieved from the major European (CGN, INRA, IPK, UPV) and Asian (AVRDC) genebanks, universities and research centres. The collection analyzed is so far the largest and the most diverse ever studied in pepper. The main objectives of this study are to: a) evaluate the genetic diversity using a large set of single-nucleotide polymorphism (SNP) data; b) detect the overlaps within collections and between genebanks; c) detect misclassification of accessions; d) develop core collections for association mapping studies. MATERIALS & METHODS Germplasm studied comprised 7,253 C. annuum accessions, 1,795 accessions belonging to the other four domesticated species (C. frutescens, C. chinense, C. baccatum, C. pubescens) and 43 accessions belonging to the wild species (Table 1). About 3% of the collection was represented by unknown species. Passport information and 42 phenotypic descriptors for plant, flower and fruit traits have been provided by genebank curators in order to enhance the description of plant material. A two restriction enzyme GBS protocol was used [1]; PstI-MspI have been selected based on in silico digestion of the reference genome accession (CM334) [2]. RESULTS GeGBS data provided more than 10,000 SNPs evenly distributed on the genome and used to characterize genetic diversity, population structure and phylogenetic relationships. The analysis allowed identifying five main clusters (K) corresponding to the most represented Capsicum species in the whole collection (Figure 1). Capsicum annuum was subdivided into two sub clusters and include admixed samples. Three other clusters were observed for C. baccatum, C. frutescens and C. chinense, respectively. The remaining species, including those corresponding to the group of purple flowers clustered in an admixed group. The principle component analysis (PCA) plot for the first two principal components suggests a geographical differentiation of the Caribbean, Central Asia and European gene-pools, and a distinction of the three main clades according to the most updated classification [3]. Moreover, the observed ~1,000 admixed accessions, and the incomplete separations between C. frutescens and C. chinense, revealed possible taxonomic misclassifications in the genebanks or gene flow between species resulting in introgressions. The genetic and phenotypic variation existing in the collection studied will be exploited for genome-wide association studies (GWAS). 178 Capsicum and Eggplant EUCARPIA Meeting 2019 | BG-O/03 DISCUSSION & CONCLUSION The genetic information provided in this study is appropriate for a deep investigation of the diversity present across the different Capsicum collections maintained by genebanks. Appropriate strategies including “germplasm genomics” can optimize the management of resources, the exchange of material between genebanks and their use for breeding purposes. Beyond genetic diversity, data can be used for the development of core collections to be exploited in GWAS, facilitating the identification of genomic regions associated with valuable traits and enhancing the efforts made by genebanks in the extensive collection and haracterization of Capsicum germplasm resources.
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titre
Genome-wide association mapping of loci involved in Potato virus Y resistance and tolerance in pepper germplasm
auteur
Lucie Tamisier, Ghislaine Nemouchi, Marion Szadkowski, Véronique Lefebvre, Emmanuel Szadkowski, Gregory Girardot, Renaud Duboscq, Sylvain Santoni, Sarah Gautier, Christopher Sauvage, Alain Palloix, Judith Hirsch, Benoît Moury
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant (2019), Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL). FRA.; Terralia. FRA.; Groupe d'Etude et de Contrôle des Variétés et des Semences (GEVES). FRA., Sep 2019, Avignon, France. 263 p
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Comparative demographic study: reconstructing the evolutionary history of Solanaceae species to better understand the domestication process and outcome.
auteur
Stéphanie Arnoux, Christelle Fraïsse, Renaud Duboscq, Mathilde Causse, Christopher Sauvage
article
Capsicum and Eggplant EUCARPIA Meeting 2019, Sep 2019, Avignon, France
resume
Background: The process of domestication is an ideal framework to study the interplay of evolutionary forces that induced molecular changes over short-time scales. These changes can now be tracked at the genome-wide scale, offering a unique opportunity to decipher the most probable demographic scenarios that occurred and characterize them. In this context and for the first time in plant, we performed a comparative genomic approach to infer the parallelism in the evolutionary history of three major crop species of the Solanaceae family, the eggplant, the pepper and the tomato. In each species, we analyzed and compared the summary statistics of two groups of crop and wild accessions, constituting prior knowledge for demographic inferences. Inferring the demographic scenarios of these three species is an unprecedented opportunity to further characterize each domestication event duration, and therefore improve the inference of the demographic history that were hypothesized through indirect means (human and cultivation history of the areas, ancient written records). Material/Methods : To infer the past demographic events, scenarios of domestication were inferred from the comparison of site frequency spectrum in crop and wild groups. We implemented over 10 demographic models to test whether the divergence between the crop and wild groups occurred (i) in the absence/presence of gene flow, (ii) with gradual/instantaneous change in their effective population size, (iii) in one or two steps. For each species, the most probable scenario was selected according to its Akaike Information Criterion, and associated parameters and their precision were estimated. These parameters were translated to human timing by the use of a range of mutation rate to avoid any bias. Abstract: Results (maximum 1300 characters spaces included) : The comparative study of the demographic inferences modeling the domestication of the three species revealed common features of the domestication processes in the Solanaceae family. In the three species the presence of a bottleneck corroborates the stage of domestication related to cultivation. That specific stage induced a reduction in effective size due to the extraction of few individuals from their wild environment to cultivate them in human managed fields. The demographic models inferred the duration of the domestication event such as the divergence time between the crop and their wild relatives, or when occurred the bottleneck. Consequently, using two mutation rates we could provide a range of estimation: the eggplant domestication occurred 5,938-3,087 years ago, the pepper sampled allowed to estimate the stage of cultivation related to a bottleneck at 6,760-3,514 years before common era (BCE), and for the tomato the domestication seems to have occurred between 7,901 and 4,107 years BCE. Another parameter was the asymmetric gene flow between the wild and crop compartment. Our results highlight that both pepper and tomato have experienced a stronger gene flow from the wild to the crop compartment than crop to wild when it is the opposite for eggplant. Discussion and conclusion Overall, our study provides insights into the convergence of the domestication processes in the Solanaceae family. Our results point out the influence of sympatry and allopatry between the crop and the wild compartments to explain the gene flows. These inferences bring as well new details about the timing of domestication and therefore insights into human history and how and when societies domesticated species. It confirms the importance of understanding how plant species respond to human manipulation. By knowing the past behavior of crops facing domestication events, we may potentially improve modern breeding efforts to sustain future crop breeding.
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Mapping the intensity function of a point process in unobserved windows
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Edith Gabriel, Jérôme Coville, Joel Chadoeuf
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GRASPA, Jul 2019, Pescara, Italy
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Predicting the intensity function of point processes inside unobserved windows
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Edith Gabriel, Francisco Rodriguez-Cortes, Jérôme Coville, Jorge Mateu, Joel Chadoeuf
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Spatial statistics, Jul 2019, Sitges, Spain
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Development and validation of an SNP-based new set of markers useful for early selection for Sharka disease in apricot ( P. armeniaca )
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P. Lambert, C. Confolent, G. Roch, N. Dlalah, A. Remay, A. Bernole, Véronique Decroocq, C. Auvinet, J.M. Audergon
article
XVII International Symposium on Apricot Breeding and Culture, ISHS, Jul 2019, Malatya, Turkey. pp.47-52, ⟨10.17660/ActaHortic.2020.1290.9⟩
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Sharka disease, due to the Plum pox virus, is a major threat to apricot trees. Most of the accessions world-wide and particularly those cultivated in Western Europe and the Mediterranean area are susceptible to the disease. A few resistance sources have been identified to date in cultivars developed in the US from a basis of Central Asian germplasm, all of these sharing the same genomic region associated with resistance to sharka in chromosome 1. Based on this, Simple sequence repeats (SSR) markers framing the MATH genes possibly responsible for the resistance have been developed in this region and a set of 2 SSRs (PGS1.21 and PGS1.24) and an indel (ZP002) was selected for identifying and discarding susceptible individuals at the plantlet stage of the apricot breeding process. However SSR markers could be difficult to implement by small teams due to the investment in hands-on time and equipment. As a consequence we turned to single nucleotide polymorphisms (SNP) to develop an alternative set of markers that could be cheaper and more easily implemented. Taking advantage of the numerous apricot genomic-sequences available at the UGAFL, we identified resistant/susceptible SNP/indel haplotypes and developed a set of three primer triplets (SP353, SP870, and SP871) based on the competitive allele-specific PCR (KASP™) technique by LGC genomics. This set was validated using a wide range of apricot accessions from different geographic origins and genetic backgrounds, concurrently with the original set of three SSRs. The new set gave similar results as the SSR-based set, was more convenient and, as a result, is now routinely used for early selection of apricot seedlings in our breeding programs.
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Evaluation of apricot fruit quality diversity in two countries, France and New Zealand
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Sylvie Bureau, C Scofield, Barbara Gouble, A Mcglone, H Boldingh, C Clark, Jean-Marc Audergon, J Stanley
article
XVII International Symposium on Apricot Breeding and Culture, Jun 2019, Malatya, Turkey
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Evaluation of apricot fruit quality diversity in two countries, France and New Zealand S. Bureau1, C. Scofield2, B. Gouble1, A. McGlone3, H. Boldingh3, C. Clark3, JM. Audergon4, J. Stanley2. 1 INRA, UMR408 Sécurité et Qualité des Produits d’Origine Végétale, Domaine Saint Paul, 228 Route de l’Aérodrome, CS 40509, F-84000 Avignon, France. 2 The New Zealand Institute for Plant and Food Research Limited, Clyde Research Centre, 990 Earnscleugh Rd, RD1, Alexandra 9391, New Zealand. 3 The New Zealand Institute for Plant and Food Research Limited, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand. 4 INRA, UR1052 Génétique et d’Amélioration des Fruits et Légumes, Domaine Saint Maurice, Allée des Chênes, CS 60094, F-84143 Montfavet cedex, France.Abstract New Zealand and French stonefruit industries desire new long-storing, sweet-tasting apricot cultivars. The climacteric nature of apricots (high ethylene production that stimulates softening via cell wall degradation) shortens their storage life. Breeders in France (FR) and New Zealand (NZ) have some apricot populations producing fruit with unusually low climacteric behavior and with a range of total soluble solids (TSS). Trials in FR and NZ shared the same research protocols, for optimal comparison and verification. Fifty genotypes representative of fruit diversity in each country were studied in June-July 2016 in FR and January-February 2017 in NZ. For each genotype, six fruit, selected according to firmness, were characterized for fruit quality traits on the day of harvest. Measurements were: intact fruit: compression firmness, Near Infrared Spectroscopy (NIRS), ethylene production, and skin color; and fruit tissue samples: Mid Infrared Spectroscopy (MIRS), TSS, titratable acidity (TA), and individual sugar and organic acid contents. A large diversity was observed for firmness, from 2 to 55 N in NZ and 2 to 36 N in FR. Ethylene production ranged from 0 to 996 nmol/h.kg in NZ and 0 to 3000 nmol/h.kg in FR, and discriminated between known low- and high-ethylene cultivars. Conversely, the variability was larger in NZ than in FR for biochemical content: TSS ranged from 8 to 24°Brix in NZ and 10 to 22°Brix in FR, and TA from 3 to 514 meq/kg in NZ and 22 to 350 meq/kg in FR. Acceptable prediction of TSS and TA was observed for NIRS and of TSS, TA, and individual sugar and organic acid contents for MIRS. NIRS and MIRS have the ability for rapid fruit characterization and discrimination across countries. The fruit genetic variability opens new opportunities for the breeding programs.
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https://hal.inrae.fr/hal-03601883/file/Turq19_Gouble_FR%20NZ%20apricot%20fruit%20diversity_abstract.pdf BibTex
titre
Genomic selection - which prospects in Prunus armeniaca?
auteur
Mariem Nsibi, Carole Confolent, Barbara Gouble, Sylvie Bureau, Alain Blanc, Guillaume Roch, Patrick Lambert, Timothée Flutre, Jean-Luc Regnard, Christopher Sauvage, Jean Marc Audergon
article
XVII International Symposium on Apricot Breeding and Culture, Jun 2019, Malatya, Turkey
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titre
Bioinformatique et reproductibilité au sein de l’unité Génétique et Amélioration des Fruits et Légumes (GAFL): mise en place et retour d'expérience.
auteur
Jacques Lagnel
article
Journées du PEPI IBIS 2019, Jun 2019, Paris, France
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titre
Tomato breeding advances in genomic era
auteur
Mathilde Causse
article
Tomato News Conference, May 2019, Avignon, France
resume
The main advances in tomato breeding are presented
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titre
Analysis of the Tomato spotted wilt virus RNA-dependent RNA polymerase adaptative evolution and constrained domains using homology protein structure modeling
auteur
Zoé Terret, Grégori Bonnet, Benoît Moury, Jean-Luc Gallois
article
17. Rencontres de Virologie Végétale (RVV 2019), Jan 2019, Aussois, France. 127 p
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https://hal.inrae.fr/hal-02735593/file/2019_Terret_RVV_1.pdf BibTex
titre
Genome-wide association mapping of loci implied in Potato virus Y resistance and tolerance in the pepper germplasm
auteur
Lucie Tamisier, Ghislaine Nemouchi, Marion Szadkowski, Gregory Girardot, Renaud Duboscq, Sylvain Santoni, Sarah Gautier, Christopher Sauvage, Alain Palloix, Judith Hirsch, Benoît Moury
article
17. Rencontres de Virologie Végétale (RVV 2019), Jan 2019, Aussois, France. 127 p
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https://hal.inrae.fr/hal-02734804/file/2019_Tamisier_RVV_1.pdf BibTex
titre
Single Primer Enrichment Genotyping Highlights the Worldwide Population Structure of Tomato and Eggplant Germplasm.
auteur
Lorenzo Barchi, Alberto Acquadro, Ezio Portis, Sergio Lanteri, David Alonso, Pietro Gramazio, Santiago Vilanova, Maria Josè Díez, Jaime Prohens, Jeremy Salinier, Véronique Lefebvre, Gancho Pasev, Stanislava Grozeva, Hatice Filiz Boyaci, Abdullah Unlu, Laura Toppino, Laura Bassolino, Giuseppe Leonardo Rotino, Andreas Boerner, Ronny Brandt, Nils Stein, Richard Finkers, Arnaud G Bovy, Roland Schafleitner, Davide Scaglione, Eleonora Di Centa, Sara Pinosio, Giuseppe Aprea, Paola Ferrante, Giovanni Giuliano
article
XXVII Plant and Animal Genome Conference, Jan 2019, San Diego (CA), United States
resume
G2P-SOL (http://www.g2p-sol.eu) is an EU-funded project, bringing together the main European and international genebanks hosting germplasm of the four major Solanaceous crops: potato, tomato, pepper and eggplant. 23,900 tomato and 5,900 eggplant accessions, including wild relatives of both crops, have been inventoried within the project. To gain information about population structure of the collections, the novel Single Primer Enrichment Technology (SPET, US Patent 9,650,628) developed by NuGEN was used for genotyping. An SNP/indel panel was developed by assaying 14k probes for tomato and 11k for eggplant, evenly distributed in the gene-rich regions, and selecting the 5k best performing probes for each species. DNA samples were prepared by the genebanks using pre-tested protocols, and genotyping partners (ENEA for tomato and University of Torino for eggplant) performed QC and sample normalization. Genotyping and sequencing was performed by IGA Technology Services. Reads were aligned to the eggplant and tomato reference genomes using BWA-MEM and SNP calling was performed using GATK-4.0. We report on the assessment of the genetic relationships in a wide set of tomato and eggplant accessions maintained in genebanks as well as the extent of duplications and possible mis-classifications. The results suggest that SPET genotyping is a reliable, high-throughput, low cost technology for genetic fingerprinting of crops, with a high degree of cross-transferability to their wild relatives. SPET-based higher density genotyping is being developed to characterize the core collections developed in G2P-SOL for GWAS analyses.
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https://hal.inrae.fr/hal-03551688/file/Barchi%202019%20PAG%20San%20Diego%20-%20oral.pdf BibTex

Other publications

titre
Durable management of plant disease resistance - What is the best strategy?
auteur
Elsa Ballini, Judith Hirsch, Benoît Moury, Loup Rimbaud, Anne-Marie Sage-Palloix
article
2019
resume
A l'INRA, l'équipe de recherche "Virologie" de Benoît Moury pose la question de l’efficacité des différentes stratégies d’utilisation des gènes de résistance aux maladies : pyramidage, rotation, mosaïque, mélange variétal…
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Poster communications

titre
Resistance against melon chlorotic mosaic virus and tomato leaf curl New Delhi virus in melon
auteur
Cecile Desbiez, Gustavo Romay, Hervé Lecoq, Catherine Wipf-Scheibel, Pauline Millot, Gregory Girardot, Michel Pitrat
article
International Advances in Plant Virology 2019, Oct 2019, Rome, Italy. , 128 p., 2019, International Advances in Plant Virology 2019 at Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Difesa e Certificazion Via C.G. Bertero 22, 00156 Roma Italy. Programme, abstracts & delegate list. 29-31 October 2019
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https://hal.inrae.fr/hal-02735214/file/2019_Desbiez_IntAdvPlantVirol_1.pdf BibTex
titre
A high-density genotyping strategy based on gene capture in pepper: perspectives for genome wide association study and genetic mapping
auteur
Sonia Elbelt, Jacques Lagnel, Bernard Caromel, Jacques David, Judith Hirsch, Emmanuel Szadkowski, Benoît Moury, Caroline Caporalino, N. Stein, G. Giuliano, Véronique Lefebvre
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France. INRA, Centre de recherche Provence-Alpes-Côte d’Azur, 263 p., 2019, Innovations in Genetics and Breeding of Capsicum and Eggplant. Proceedings of the 17th EUCARPIA Meeting on Genetics and Breeding of Capsicum and Eggplant September 11-13, 2019 | Avignon - France
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https://hal.inrae.fr/hal-02737663/file/2019_Elbelt_Eucarpia_1.pdf BibTex
titre
Diversity of Tobacco etch virus pathotypes faced with pepper resistance sources and durability potential of resistance genes
auteur
Benoît Moury, Bülent B. Arpaci, Vincent Simon, Alain Palloix
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France. INRA, Centre de recherche Provence-Alpes-Côte d’Azur, 263 p., 2019, Innovations in Genetics and Breeding of Capsicum and Eggplant. Proceedings of the 17th EUCARPIA Meeting on Genetics and Breeding of Capsicum and Eggplant September 11-13, 2019 | Avignon - France
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https://hal.inrae.fr/hal-02737252/file/2019_Moury_Eucarpia_1.pdf BibTex
titre
The fascinating diversity of pepper and eggplant collections held at the INRA Centre for Vegetable Germplasm
auteur
Jérémy Salinier, Marie-Christine Brand-Daunay, J Goussopoulos, C Gros, Véronique Lefebvre
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant (2019), Sep 2019, Avignon, France
resume
The INRA Unit for Genetics and Breeding of Fruit and Vegetables has carried out genetic research and breeding on pepper (Capsicum annuum L.) and eggplant (Solanum melongena L.) since 1962 and has progressively gathered numerous genetic resources, which are now managed by the INRA Centre for Vegetable Germplasm. Our goal is to present a comprehensive overview of the current content of both collections, including their wild and cultivated relatives.
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https://hal.inrae.fr/hal-02958665/file/The%20Fascinating%20Diversity%20of%20Pepper%20and%20Eggplant%20Collections%20held%20at%20the%20INRA%20Centre%20for%20Vegetable%20Germplasm.pdf BibTex
titre
Genetic architecture of robustness in Capsicum annuum for resistance to Phytophthora capsici and Potato virus Y under a temperature stress
auteur
Valentin Ribaut, Anne Massire, Judith Hirsch, Emmanuel Szadkowski, Loup Rimbaud, Bernard Caromel, Alexandre Bachellez, Nasradin Touhami, Marion Szadkowski, Benoît Moury, Véronique Lefebvre
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France. INRA, Centre de recherche Provence-Alpes-Côte d’Azur, 263 p., 2019, Innovations in Genetics and Breeding of Capsicum and Eggplant. Proceedings of the 17th EUCARPIA Meeting on Genetics and Breeding of Capsicum and Eggplant September 11-13, 2019 | Avignon - France
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https://hal.inrae.fr/hal-02736058/file/2019_Ribaut_Eucarpia_1.pdf BibTex
titre
Phytophthora capsici aggressiveness toward pepper and tomato
auteur
Emmanuel Szadkowski, Alexandre Bachellez, Nasradin Touhami, Anne Massire, Dylan Storey, Kurt Lamour, Véronique Lefebvre
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant (2019), Sep 2019, Avignon, France. , 2019
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titre
Deciphering the genetic basis of tolerance to Cucumber mosaic virus in pepper
auteur
Judith Hirsch, Marion Szadkowski, Gregory Girardot, Allan Kampfer, Maxime Louis, Sandra Martin, Sabrina Azehaf, Yseult Cardona, Alexandra Schoeny, Pauline Millot, Catherine Wipf-Scheibel, Patrick Gognalons, Cecile Desbiez, Karine Nozeran, Eric Verdin, Alain Palloix, Benoît Moury
article
17. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France. INRA, Centre de recherche Provence-Alpes-Côte d’Azur, pp.88, 2019, Innovations in Genetics and Breeding of Capsicum and Eggplant. Proceedings of the 17th EUCARPIA Meeting on Genetics and Breeding of Capsicum and Eggplant September 11-13, 2019 | Avignon - France
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https://hal.inrae.fr/hal-02737621/file/2019_Hirsch_Eucarpia_Capsicum_and_Eggplant.pdf BibTex
titre
Development of genome-wide KASPar markers from a comparative transcriptomic analysis for mapping QTLs associated with the pungent trait of pepper (Capsicum annuum L.)
auteur
Lihao Wang, J.P. Bouchet, Wang N, Zhang Z, A.M. Sage-Palloix, Y Cao, Zhang B, Alain Palloix, Véronique Lefebvre
article
17th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France
resume
BACKGROUND Pepper (Capsicum spp.), belonging to the Solanaceae family, is an important vegetable crop worldwide owing to its nutritional value and flavor. There are five domesticated pepper species (C. annuum L., C. frutescens L., C. chinense Jacq., C. baccatum L., and C. pubescens Ruiz et Pav.) and more than 20 wild relative species. Global pepper production is approximately 34.5 million tons annually.Under the threat of a changing environment, new varieties with enhanced resistance to biotic and abiotic stresses are demanded by farmers. Marker-assisted selection (MAS) has been used to introduce new and desirable characters into plants, such as disease resistance. The lack of sufficient molecular markers for pepper has been a bottleneck for genetic and linkage studies [1]. MATERIALS & METHODS Transcriptomes from five C. annuum L. accessions (Yolo Wonder, CM334, Perennial, PM687 and PM217) were sequenced. Unigenes of CM334, Perennial, PM687, and PM217 were mapped to the reference transcriptome of Yolo wonder [1]. Positions of SNPs and their flanking sequences were extracted to design KASPar markers. A genetic map based on KASPars was constructed by using Joinmap 4 thanks to 113 F9-recombinant-inbred-lines (RIL) issued from the intraspecific cross ‘Perennial x 83-58’. Capsaicin and dihydrocapsaicin were quantified in the RIL progeny by ultra-high performance liquid chromatography (UPLC, Waters, USA) and a QTL analysis was performed by using the software MapQTL3.0. RESULTS Comparison of the erennial transcriptome with the Yolo Wonder transcriptome revealed 9,037 SNPs corresponding to 4,333 unigenes. Comparison of the transcriptomes of CM334, PM687, and PM217 with the Perennial transcriptome revealed 2,077 SNPs corresponding to 1,674 unigenes. We developed KBioscience Competitive Allele-Specific PCR (KASPar) markers for a set of SNPs. Out of the 975 KASPars developed, 303 (31%) were polymorphic between Perennial and 83-58. We constructed a genetic linkage map with 372 markers (including 278 KASPars) and detected quantitative trait loci (QTL) for the pungent trait (capsaicin and dihydrocapsaicin content) using the F9 RIL progeny. Four QTLs determining the capsaicin and dihydrocapsaicin content were identified. For traits related to the capsaicin content, one major QTL was located between marker BD76366 and Pun1 on chromosome P2; it explained 29% to 41.5% of the phenotyptic variation. Two minor QTLs located on chromosome P2 and P12 explained 8.0% and 11.0%. For the dihydrocapsaicin content, one major and two minor QTLs were located at the same positions as the QTLs of capsaicin content on chromosomes P2 and P12; an additional minor QTL located on chromosome P12 explained 9.9% of the phenotypic variation. DISCUSSION & CONCLUSION This research identified thousands of polymorphic positions among the genomes of pepper accessions. We developed KASPar markers covering the whole-genome and that can be used to locate QTLs or genes at any position. 31% of 975 KASPar markers were polymorphic; they were used to construct a molecular linkage map and identified QTLs associated with the pungent trait. The KASPar markers we have developed have potential value for pepper crop improvement and breeding for altered capsaicin content.
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https://hal.inrae.fr/hal-03551775/file/WangL%20%202019%20Eucarpia%20poster.pdf BibTex
titre
COMPARISON BETWEEN TRADITIONAL AND MODERN FRESH MARKET TOMATO VARIETIES IN THREE EUROPEAN COUNTRIES: SENSORY ANALYSIS AND GENE EXPRESSION PROFILING
auteur
Fiorella Sinesio, Brigitte Navez, Mathilde Causse, Joan Casals, Romero del Castillo, Saggia Civitelli, Valentine Cottet, Antonio Granell, Silvana Grandillo
article
LXIII SIGA ANNUAL CONGRESS, Sep 2019, Napoli, Italy
resume
Tomato (Solanum lycopersicum L.) is one of the most important crops worldwide. Sensory quality of fresh market tomatoes is defined by numerous traits relating to appearance, flavour (taste and aroma) and textural properties. Widespread consumer complaints with the flavor quality of many modern commercial tomato varieties for fresh market have urged sensory quality to become a major breeding objective, and have also fostered the development of niche markets for heirloom, local or traditional varieties. In order to meet consumers’ demand, new breeding targets should be identified based on consumers’ preferences. To gain further insights into the factors underlying tomato sensory quality and driving consumers’ liking, within the framework of the European TRADITOM project (http://traditom.eu/), 17 traditional and modern fresh market tomato varieties, belonging to the typologies “Oxheart”, “Marmande” and “Round” were selected and different partially overlapping sets were grown and evaluated in France, Italy and Spain. Two different growing conditions were tested in France (soilless under greenhouse (HS) vs. soil under plastic tunnel (S)) and in Spain (open air (OA) vs. soil under greenhouse (G)), while in Italy the products were grown in soil under plastic tunnel, and were evaluated at two different ripening stages. In each country fruit quality was assessed at three levels: objective description of sensory properties by trained panels, consumer preference tests and physico-chemical measurements. To determine how consumers perceived the sensory characteristics of the different products, after the overall liking (OL), they were asked to answer a Check-All-That-Apply (CATA) survey, with 24 questions related to fruit appearance, flavor and texture. Principal component analysis (PCA) was carried out to describe the relationships between physicochemical and sensory variables and to identify sample groups. Overall a strong genotype effect on consumer OL was observed; while the effect of different growing conditions on OL was not significant. Correspondence Analysis run on the contingency tables of CATA attributes from each country allowed finding associations between tomato varieties and CATA attributes. Finally, transcriptional profiling of selected genes was conducted on all the samples by a mid-throughput Fluidigm RT-PCR platform. The main sensory and transcriptional profiling results will be discussed. This work was supported by the European Commission Horizon 2020 program (TRADITOM grant agreement no. 634561).
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titre
Deciphering the machinery controlling the degradation of oleosins, the gatekeepers of seed lipid droplets
auteur
Sabine D’andrea, Carine C. Deruyffelaere, Isabelle Bouchez, Jean-Luc Cacas, Jean-Luc Gallois
article
9th European Symposium on Plant Lipids, Jul 2019, Marseille, France
resume
Seed lipid droplets (LDs) of many dicotyledonous plants, including Arabidopsis thaliana, are a good model to investigate the mechanisms controlling LD dynamics. Indeed, LD degradation occurs rapidly and massively in germinated seeds, to provide carbon and energy for seedling growth. Post-germinative mobilization of storage lipids is preceded by the degradation of oleosins, the major structural LD proteins which stabilize LDs in dry seeds. We previously showed that oleosins are marked for degradation by ubiquitination, and extracted from LDs before proteolysis. The mechanisms underlying the dislocation of these LD-anchored proteins from the phospholipid monolayer are yet unknown. We found that a protein called PUX10 is required for proper extraction of ubiquitinated oleosins from the LD surface in young Arabidopsis seedlings. PUX10 localizes to LDs and binds to ubiquitinated proteins. PUX10 also interacts with CDC48A, the ATPase which selectively extract misfolded proteins from the ER in the degradation process known as ERAD (for ER-associated degradation). We propose that PUX10 is an adaptor protein recruiting CDC48A to ubiquitinated oleosins, thus promoting the extraction of oleosins from LDs by the segregase activity of CDC48A. PUX10 and CDC48A are core components of a new LD-associated machinery that we named LD-associated degradation (LDAD) system. Moreover, we observed that PUX10 and CDC48A localize to a specific subpopulation of LDs in germinated seeds, suggesting a functional differentiation of LDs in plants. We also showed that a deubiquitinating enzyme known for its role in intracellular trafficking and vacuolar biogenesis is necessary for the ubiquitination of oleosins.
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https://hal.science/hal-04518499/file/20190707_EuroPlantLipids_Marseille_DAndrea.pdf BibTex
titre
Development and validation of an SNP-based new set of markers useful for early selection for sharka disease in apricot (P. armeniaca)
auteur
Patrick Lambert, Carole Confolent, Guillaume Roch, Naïma Dlalah, Arnaud A. Remay, Anne Bernole, Véronique Decroocq, Clarisse Auvinet, Jean Marc Audergon
article
17. International Symposium on Apricot Breeding and Culture, Jul 2019, Malatya, Turkey. , pp.1, 2019
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https://hal.inrae.fr/hal-02790319/file/SAM%20sharka_XVII%20apricot%20symposium_Lambert_1.pdf BibTex
titre
AllMine, a flexible pipeline for allele mining
auteur
Thomas Bersez, Jean-Luc Gallois, Jacques Lagnel
article
JOBIM (Journées Ouvertes Biologie, Informatique et Mathématiques), Jul 2019, Nantes, France
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titre
Lettuce lines with potyvirus resistance: differential set for study of strains and new genitors of resistance
auteur
Brigitte Maisonneuve, Yannick Bellec, Eric Martin, Hervé Lot, Patrick Gognalons, Benoît Moury
article
9. International conference on genetics and breeding of leafy vegetables. Eucarpia Leafy Vegetables 2019, Jun 2019, Olomouc, Czech Republic. JOLA, v.o.s., pp.44-45, 2019, 9th International Conference on Genetics and Breeding of Leafy Vegetables. Eucarpia Leafy Vegetables 2019. Programme and Proceedings of Abstracts
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https://hal.inrae.fr/hal-03553254/file/Maisonneuve_HAL-INRAE.pdf BibTex
titre
Selections issued from CEP Innovation and CENTREX and INRA - Innovative apricot breeding program in France.
auteur
C Auvinet, C Pitiot, G Roch, A Blanc, F Gilles, G Clauzel, J Broquaire, B Gouble, S Bureau, Jean-Marc Audergon
article
XVII International Symposium on Apricot Breeding and Culture, Jun 2019, Malatya, Turkey. 1p
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https://hal.inrae.fr/hal-03600730/file/Turq19_Auvinet_Innovating%20breeding_poster.pdf BibTex
titre
Deciphering the genetic basis of tolerance to Cucumber mosaic virus in pepper
auteur
Judith Hirsch, Marion Szadkowski, Gregory Girardot, Allan Kampfer, Maxime Louis, Sandra Martin, Sabrina Azehaf, Yseult Cardona, Alexandra Schoeny, Pauline Millot, Catherine Wipf-Scheibel, Patrick Gognalons, Cecile Desbiez, Karine Nozeran, Eric Verdin, Alain Palloix, Benoît Moury
article
17. Rencontres de Virologie Végétale (RVV 2019), Jan 2019, Aussois, France. 127 p., 2019, 17èmes Rencontres de Virologie Végétale : RVV 2019
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https://hal.inrae.fr/hal-02735594/file/2019_Hirsch_RVV_1.pdf BibTex
titre
Identification and characterization of new resistance to viruses in Cucurbitaceae
auteur
Aimeric Agaoua, Cecile Desbiez, Verane Sarnette, Vincent Rittener, Catherine Dogimont
article
17. Rencontres de Virologie Végétale (RVV 2019), Jan 2019, Aussois, France. 127 p., 2019, 17èmes Rencontres de Virologie Végétale : RVV 2019
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Reports

titre
D2.2 Quality annotation protocols for phenotypic platform data
auteur
Nadine Hilgert, Isabelle Sanchez, Emilie J. Millet, Fred van Eeuwijk
article
D2.2, EPPN2020. 2019, 15p
resume
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 731013. This publication reflects only the view of the author, and the European Commission cannot be held responsible for any use which may be made of the information contained therein.

EPPN 2020. The present deliverable specifically addresses quality annotation protocols for phenotyping platform data. We first explain what cleaning phenotypic data is and why it is important to do it and keep track of how it was done. We then provide platform users with clearly described and defined rules for outliers identification and annotation in an automatic and traceable way. An outlier is usually defined as an observation that appears to be inconsistent with the remainder of the dataset. After visiting a number of facilities and discussing with platform users, we have defined three types of outliers to annotate in the phenotypic data: (1) time points within a time course, (2) whole time courses of one or more variables and (3) a whole plant, defined here as a biological replicate deviating from the overall distribution of plants on a multi-criteria basis. This classification of outliers was proven relevant by the consortium partners. In this document, we propose procedures to identify them. For the first two types of outliers, statistical methods already exist and have been adapted and applied to datasets from different platform/species. The «plant outlier» type is new and a method has recently been published (Alvarez Prado et al., 2019). The common idea here is to provide annotated data to the user who, in the end, will decide whether or not to keep the annotated points, time course or plant for further analyses.

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https://hal.inrae.fr/hal-04976453/file/EPPN2020_D2.2-validated.pdf BibTex
titre
Réflexion prospective interdisciplinaire. Approches prédictives pour la biologie et l’écologie. Rapport de synthèse.
auteur
Carole Caranta, Hervé Monod, Hugues Berry, Michaël Chelle, Michel Génard, Fabien Jourdan, Christian Lannou, Emmanuelle Maguin, Françoise Médale, Sylvie Oddou-Muratorio, Claire Rogel Gaillard, Jan Traas
article
[0] INRA. 2019, 40 pp
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https://hal.inrae.fr/hal-02791153/file/0-RapportBiolPredict_Dec2019_final.pdf BibTex

2018

Conference papers

titre
Contribution of Oxford NanoPore technology for the de novo genome assembly of nonmodel species: the case of the Asian ladybird Harmonia axyridis
auteur
Jacques Lagnel, Arnaud A Estoup, Cecile Donnadieu, Celine Lopez-Roques, Maxime Manno, Anne Loiseau, Mathieu Gautier
article
Réunion France Génomique, Nov 2018, Evry, France
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titre
Aphid triggered immunity in melon: key determinants for durable resistance to virus and aphids
auteur
Nathalie Boissot
article
Cucurbitaceae 2018, University of California [Davis] (UC Davis). USA., Nov 2018, Davis, United States
resume
The Vat gene in melon is unique in conferring resistance to both A. gossypii and the viruses it transmits. This double phenotype is controlled by a cluster of genes including a CC-NLR which has been characterized in detail. Copy-number polymorphisms (for the whole gene and for a domain that stands out in the LLR region) and single-nucleotide polymorphisms have been identified in the Vat cluster. The Vat gene structure suggests a functioning so called effector-triggered immunity (ETI), with separate recognition and response phases. During the recognition phase, the VAT protein is thought to interact (likely indirectly) with an aphid effector introduced by aphid salivation within the plant cells. A few hours later, several miRNAs are upregulated in Vat plants. Peroxidase activity increases, and callose and lignin are deposited in the walls of the cells adjacent to the stylet path, disturbing aphid behavior. In aphids feeding on Vat plants, the levels of miRNAs are modified. At the plant level, resistance to aphids is quantitative (aphids escape the plant and display low rates of reproduction). ‘Aphid-ETI’ has qualitative and local effect against non circulative viruses (CMV, ZYMV, WMV) and but quantitative effect against circulative virus such as CABYVs. Durability of ETI is highly variable. At population level, ‘Aphid-ETI’ reduces aphid density and genetic diversity, and durability of the ‘Aphid-ETI’ strongly depends on the agro-ecosystem. Some clones are adapted to Vat resistance, putatively either by introducing a polymorphic effector not triggering ETI, or by adapting to the defenses they triggered. Several ways to enhance ‘Aphid-ETI’ durability will be proposed. ‘Aphid-ETI’ against viruses decreases the intensity of Cucumber Mosaic Virus (CMV) and Cucurbit aphid-borne yellows virus (CABYV) epidemics. Laboratory experiments strongly suggested that non circulative viruses cannot adapt to ‘Aphid-ETI’ and therefore durability of ‘Aphid-ETI’ against CMV is predicted long. Field experiments combined to modelling suggested that highly durable resistance against CABYV could be obtained by combining within a same melon genotype the Vat gene with recessive genes conferring resistance to CABYV. Extension of those results to any other circulative viruses will be discussed.
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https://hal.inrae.fr/hal-02734670/file/Cucurbit%202018%20Summary%20NBoissot_1.pdf BibTex
titre
Barcoding pour étudier les forces sélection/dérive génétique dans des populations de puceron
auteur
Rafael Feriche-Linares, Nathalie Boissot
article
Detection, Gestion et Analyse du Polymorphisme des Genomes Végétaux, EPGV, Oct 2018, Evry, France
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https://hal.inrae.fr/hal-03973500/file/2018-%20Nboissot%20Evry.pdf BibTex
titre
The G2P-SOL project: harnessing the genetic and phenotypic diversity of the four major Solanaceae crops
auteur
Giovanni Giuliano, Véronique Lefebvre, , On Behalf of The G2p-Sol Consortium
article
15th Solanaceae Conference, Sep 2018, Chiang Mai, Thailand
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titre
Single Primer Enrichment genotyping highlights the worldwide population structure of tomato and eggplant germplasm.
auteur
Lorenzo Barchi, A. Acquadro, Ezio Portis, Sergio Lanteri, David Alonso, P. Gramazio, Santiago Vilanova, Maria José Diez, Jaime Prohens, Jérémy Salinier, Véronique Lefebvre, Laura Toppino, L. Bassolino, Giuseppe L. Rotino, D. Scaglione, Dicenta E., S. Pinosio, Giuseppe Aprea, Paola Ferrante, Giovanni Giuliano
article
15th Solanaceae Conference, Sep 2018, Chiang Mai, Thailand
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PUX10 associates with CDC48A and regulates the extraction of ubiquitinated oleosins from seed lipid droplets
auteur
Carine Deruyffelaere, Isabelle Bouchez, Jean-Luc Cacas, Jean-Luc Gallois, Sabine D’andrea
article
14th GERLI Lipidomics Meeting, Sep 2018, St Maximin la Saint-Baume, France
resume
Seed lipid droplets (LDs) of many dicotyledonous plants, including Arabidopsis thaliana, are a good model to investigate the mechanisms controlling LD dynamics. Indeed, LD degradation occurs rapidly and massively in germinated seeds, to provide carbon and energy for seedling growth. Post-germinative mobilization of storage lipids is preceded by the degradation of oleosins, the major structural LD proteins which stabilize LDs in dry seeds. We previously showed that oleosins are marked for degradation by ubiquitination, and extracted from LDs before proteolysis. The mechanisms underlying the dislocation of these LD-anchored proteins from the phospholipid monolayer are yet unknown. Here, we report that a novel LD-associated protein, called PUX10, control this process. We phenotypically characterized and functionally complemented two mutants of PUX10 in Arabidopsis. Using biochemical approaches, we showed that PUX10 deficiency delays the degradation of oleosins and ubiquitinated oleosins, and prevents the extraction of ubiquitinated oleosins from the LD surface. We also identified several functional domains by expressing truncated versions of PUX10 in tobacco leaves or in yeast. In addition to a membrane-anchoring domain, PUX10 possesses two functional UBA and UBX domains that mediate interactions with ubiquitin and the CDC48A AAA-ATPase, respectively. We propose that PUX10 is a LD-anchored scaffolding protein recruiting CDC48A to ubiquitinated oleosins, thus promoting the dislocation of oleosins from LDs by the segregase activity of CDC48A. This mechanism is reminiscent of the ER-associated degradation (ERAD) translocation process but localizes to LDs; we therefore propose to name it LD-associated degradation (LDAD). Importantly, our comprehensive analysis of PUX10 localization using Arabidopsis transgenic lines expressing PUX10-GFP under native promoter revealed that PUX10 is progressively expressed and sequentially targeted to a specific subpopulation of LDs in germinated seeds. PUX10 is therefore the first determinant of a LD subpopulation identified in plants, suggesting a functional differentiation of LDs.
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Impact of heating location, forced ventilation and screens on the energy efficiency and condensation risks inside a cucumber greenhouse.
auteur
Pierre-Emmanuel Bournet, Eric Brajeul, Vincent Truffault, Etienne E. Chantoiseau, R. Naccour
article
XXX International Horticultural Congress IHC2018: III International Symposium on Innovation and New Technologies in Protected Cultivation, International Society for Horticultural Science: ISHS, Aug 2018, Istambul, Turkey. 8p., ⟨10.17660/ActaHortic.2020.1271.4⟩
resume
In the West of France, greenhouse cucumber production is largely developed. Heating makes it possible to maintain a high level of production, but with high energy costs. Growers therefore try to implement new crop management practices to increase the energetic efficiency while keeping good productivity and quality. In this prospect, two treatments were tested considering two greenhouse compartments (307 m2): one with heating tubes at the upper limit of the canopy and a thermal screen (control) and the second one with heating tubes at low position inside the canopy, mechanical ventilation (5 volumes h-1) and thermal + shading screens (innovative). These compartments were equipped with sensor chains, each with four pairs of temperature and humidity probes at four levels. Additional thermocouples measured fruit temperatures. The energy consumption of the compartments was also assessed. Measurements were conducted over a three-month period of time. Results show that a better homogenization of the distributed climate was reached inside the innovative treatment. Also, the innovative treatment made it possible to significantly reduce risks of condensation on fruit compared to the control. Finally, in the innovative compartment, the energy efficiency was notably increased compared to the conventional management, generating the same yield, but saving 17% of the energy. The innovative crop management therefore demonstrated the benefits of the fans combined with low level heating and double screens.
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OSCAR, a national observatory to support the deployment of new grapevine disease resistant varieties in France
auteur
François Delmotte, Soizic Guimier, Isabelle Demeaux, Carole Couture, Christophe J. Schneider, Rémy Cailliatte, Laurent Audeguin, Frédéric Fabre, Laurent Deliere
article
12. International Conference on Grapevine Breeding and Genetics (ICGBG), Jul 2018, Bordeaux, France. 306 p
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Apport de la technologie Oxford NanoPore pour l’assemblage de novo du génome d’espèces non-modèles : le cas de la coccinelle asiatique Harmonia axyridis
auteur
Jacques Lagnel, Arnaud A Estoup, Cecile Donnadieu, Celine Lopez-Roques, Maxime Manno, Anne Loiseau, Mathieu Gautier
article
Journées Ouvertes en Biologie, Informatique et Mathématiques (JOBIM 2018), Société Française de Bio-Informatique (SFBI). FRA. GdR 3003 Bioinformatique Moléculaire (GdR BiM), FRA. Institut Français de Bioinformatique (IFB), FRA. Aix Marseille Université (AMU), FRA. Institut National de la Santé et de la Recherche Médicale (INSERM), FRA. Centre National de la Recherche Scientifique (CNRS), FRA., Jul 2018, Marseille, France
resume
La technologie de séquençage long fragments d'Oxford NanoPore (ONT) est basée sur le séquençage des acides nucléiques via le passage de molécules uniques au travers de nano pores protéiques et permet l’obtention de séquences de plusieurs dizaines de kilobases. Elle a très récemment connu une phase exponentielle de développement et son champ d'application s’est élargi à de nombreux domaines de la génomique et notamment l’assemblage de génome de novo. La coccinelle H. axyridis originaire d'Asie, a été introduite pour être utilisée comme agent de lutte biologique mais a établi des populations invasives en Amérique, en Europe et Afrique du Sud. H. axyridis est devenue une espèce modèle pour l’exploration du déterminisme génétique des caractères impliqués dans le succès invasif de certaines populations. D’autre part, avec plus de 200 morphes de couleur d'élytres contrôlés par un seul locus multi-alléliques, H. axyridis est également une espèce emblématique pour l’étude du déterminisme génétique du polymorphisme de couleur. Néanmoins, mener à bien la caractérisation de l’architecture génétique de ces différents caractères d’intérêt nécessite de disposer de ressources génomiques riches et notamment d’assemblages de génome de bonne qualité (la taille haploïde du génome de H. axyrridis étant estimée à 400 Mb). Suite aux résultats relativement décevants en termes de qualité d’assemblage obtenus à partir de données Illumina classiques (séquençage de banques en PE et MP), nous avons généré des séquences longues issues de la technologie ONT (4 flowcells MinIon, 65X, GeT-PlaGe, INRA-Genotoul, France). Un assemblage de novo a été réalisé en combinant ces longs reads ONT avec des reads courts d’Illumina. Plus précisément, le pipeline d'assemblage comprenait une correction des reads initiaux avec le programme bioinformatique Canu suivi d’un assemblage avec SMARTdenovo. L’assemblage ainsi généré présentant un taux riche en délétions d'homopolymères, une étape de polissage a été réalisée avec des lectures Illumina (banques PE et MP). In fine, la contiguïté de notre assemblage est 200 fois supérieure à notre assemblage initial. La moitié du génome obtenu est contenue dans des séquences génomiques de plus de 1,4 Mb avec une complétude évaluée par l’alignement de gènes orthologues conservés (BUSCO) de plus de 95.5%. Enfin, un contig de 1.3Mb nous a permis une résolution moléculaire fine d’une région du génome de 200 kb caractérisée par un grand nombre de SNPs signant des variations moléculaires importantes spécifiques des différents morphes de couleur d’H. axyridis. L’utilisation de la technologie ONT pour l’assemblage de novo de notre espèce non-modèle s’est ainsi révélée d’un excellent rapport qualité/prix.
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Exploring the genetic variability of peach skin phenolics and triterpenoids as natural defenses against brown rot
auteur
Bénédicte Quilot-Turion, Silvia Scariotto, Marie Noëlle Corre, Leandro de Oliveira-Lino, Véronique Signoret, Maximiliano Dini Vinoly, Lorraine Latchoumane, Maria Do Carmo Raseira, Jean-Luc Poëssel
article
9. International Rosaceae Genomics Conference, Nanjing Agricultural University (NAU). Nanjing, CHN., Jun 2018, Nanjing, China
resume
Brown rot caused by Monilinia spp. provokes dramatic losses of peach in all production regions. In this context, the general objective of our work is to develop sustainable ways to fight against this damaging disease. This study explored phenolic and triterpenoid diversity of fruit skin together with brown rot resistance. A Brazilian peach collection and an interspecific cross between P. davidiana and P. persica, each of 120 genotyped individuals, have been screened for 3 years, for susceptibility to M. fructicola and to M. laxa respectively. Some genotypes of the two populations showed low or null infection the 3 years. Secondary compounds of fruit skin of all genotypes were analyzed two years by HPLC-DAD. Depending on the genotypes, 30 to 40 compounds including triterpenoids, hydroxycinnamic derivatives and flavonoids, were quantified and around 20 were identified by mass spectrometry. Among these, phenolic esters of triterpenoids were identified for the first time in peach fruit. The correlation of quantitative data between years showed their robustness. A large diversity in contents of the compounds was observed. The association and QTL analyses proved to be complementary and led to assumptions about the co-locations of loci controlling different traits, especially for compounds with differential levels in the skin of peaches and nectarines. These analyses will help deciphering the genetic control of fruit skin phenolics and triterpenoids and pave the way to the identification of the underlying genes. Detection of loci related to resistance will provide tools for the implementation of marker assisted selection. Correlations between compounds and infection traits led us to hypothesize that some compounds from peach skin could play a role in the brown rot control. The fungicide activity of these compounds is being tested in vitro.
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https://hal.inrae.fr/hal-02791339/file/C387B32D-49F8-4752-8155-1DE74807FE2F_1 https://hal.inrae.fr/hal-02791339/file/Abstract_2 BibTex
titre
Resistance to sharka in Stone Fruit trees : Genetic and genomic technologies for new breeding strategies
auteur
Véronique Decroocq, Stephane Decroocq, David Tricon, Timothée Flutre, Thierry Pascal, Christophe Tuero, J. Salava, S. Liu, W.S. Liu, J. Rubio, E. Sanchez, H. Prieto
article
9. International Rosaceae Genomics Conference, Jun 2018, Nanjing, China
resume
Resistance to sharka in Stone Fruit trees : Genetic and genomic technologies for new breeding strategies. 9. International Rosaceae Genomics Conference
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https://hal.science/hal-01819033/file/DecroocqV%20Resistance%20to%20sharka%20in%20Stone%20Fruit%20trees_1.pdf BibTex
titre
Genomic selection - which prospects in Prunus armeniaca? Preliminary results issued from fruit quality traits and phenology
auteur
Mariem Nsibi, Carole Confolent, Barbara Gouble, Sylvie Bureau, Alain Blanc, Guillaume Roch, Patrick Lambert, Timothée Flutre, Jean-Luc Regnard, Christopher Sauvage, Jean Marc Audergon
article
9. International Rosaceae Genomics Conference, Jun 2018, Nanjing, China
resume
Genomic selection (GS) refers to a selection method which intends to assess individuals according to their genomic values using genome-wide dense markers. Particularly efficient in animal improvement strategies, opening interesting perspective in crops, it is already engaged in perennial species such as apple where, genetic gain was maximized for fruit quality traits quantitatively inherited by comparison with conventional breeding strategy. In the present work, GS was applied on a biparental apricot population (184 individuals) characterized over two consecutive years for phenology (blooming and maturity dates) and fruit quality traits (fruit weight, color, sugar nd acidity content, ethylene production). A ridge regression (RR-BLUP) modelling has been used. The performance of GS was assessed by cross validation using the accuracy defined as the correlation between true phenotypes values and the estimated ones. The effects of markers density and number, training set size and composition as well as the heritability of the investigated traits were evaluated on the accuracy of the model. The main results issued from the two years multi-annual modelling approach are showing that (i) genomic predictions are accurate even with a little number of markers (>100 markers), (ii) the accuracy increases with the training population’s size and with high heritability traits, (iii) and the optimization of the training set improves the performance of genomic selection model. To conclude, a clear interest exists in continuing the evaluation of the genomic selection in biparental apricot populations even for traits that are difficult to measure. The robustness of the approach needs to be tested both on other progenies and on a large set of genetic resources
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https://hal.science/hal-01830311/file/Apricot%20Selgen_MNsibi_Abstract_1.pdf BibTex
titre
Lutte contre les pucerons des cultures maraichères sous abris : Méthode de production d’auxiliaires
auteur
Christophe Giraud
article
Rencontre EMBA (Ecological Management of Bioagressors in Agroecosystems) 2018, Jun 2018, Montpellier, France
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Towards an integrative modeling approach for peach ideotype design adapted to sustainable agriculture
auteur
Bénédicte Quilot-Turion
article
2. trilateral INUPRAG symposium, Umea Plant Science Centre (UPSC). SWE., Jun 2018, Barcelone, Spain
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https://hal.inrae.fr/hal-02791337/file/UNIPRAG%202018%20Meeting%20Program_1 BibTex
titre
Pest and pathogen resistance in peach
auteur
Patrick Lambert
article
Organic Farming Reasearch and Perspectives (ORGANIST), May 2018, Milan, Italy. pp.24
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https://hal.inrae.fr/hal-02791308/file/2018_Lambert_Organist_1.pdf BibTex
titre
eIF4E-mediated resistance to viruses: from natural variation to synthetic alleles
auteur
Jean-Luc Gallois, Anna Bastet, Camille Gauffier, Christophe Robaglia, Carole Caranta
article
Embo workshop Target of rapamycin (TOR) signaling in photosynthetic organisms, EMBO. Heidelberg, DEU., May 2018, Bischoffsheim, France
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Genomic selection for fruit quality and yield in tomato.
auteur
Jiantao Zhao, Christopher Sauvage, Mathilde Causse
article
XIX EUCARPIA Meeting of the Tomato Working Group., May 2018, Napoli, Italy
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https://hal.inrae.fr/hal-03543135/file/Zhao_Abstract_form_EUCARPIA%20TOMATO%202018.pdf BibTex
titre
Genotype by environment interaction in tomato grown under several abiotic stresses and the genetic basis of plasticity
auteur
Isidore Diouf, Laurent Derivot, Shai Koussevitzky, Yolande Carretero, Frédérique Bitton, Mathilde Causse
article
XIX EUCARPIA Meeting of the Tomato Working Group, May 2018, Napoli, Italy
resume
Abiotic stresses and cultural conditions can lead to different performances of a given genotype when exposed to different environments. The ability of a genotype to change its phenotype is called phenotypic plasticity and has received great interest these last decades. Plant geneticists are aware of the importance of measuring phenotypic plasticity and assessing its genetic basis for breeding purposes. In order to analyse the impact of several stress conditions at the genetic level, we grew a multi-parental tomato population in12 distinct environments. The environments that were located in Israel, France and Morocco comprised optimal cultural conditions and water deficit, salinity and heat stress conditions. Quantitative trait loci (QTL) mapping analysis was performed on these data in three step: (i) main effect QTL were mapped on the trait means for each environment separately; (ii) QTL x Treatment effect were mapped on ‘Treatment specific plasticity’ measured as the difference between the optimal and stressed treatment; (iii) plasticity QTL were mapped based on the Finlay–Wilkinson’s regression slope. Results of phenotypic analysis and QTL detection will be presented as well as some attempt at identifying candidate genes for QTL for plasticity traits.
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https://hal.inrae.fr/hal-03562885/file/Causse_oral_Abstract_form_EUCARPIA%20TOMATO%202018.pdf BibTex
titre
Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance
auteur
Anna Bastet, Baptiste Lederer, Nathalie Giovinazzo, Xavier Arnoux, Sylvie German-Retana, Catherine Reinbold, Véronique Brault, Damien Garcia, Samia Djennane, Sophie Gersch, Olivier Lemaire, Christophe Robaglia, Jean-Luc Gallois
article
20. Journées Francophones de Virologie 2018, Société Française de Virologie (SFV). FRA., Mar 2018, Paris, France
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Apport de la technologie Oxford NanoPore pour l’assemblage de novo du génome d’espèces non-modèles : le cas de la coccinelle asiatique Harmonia axyridis
auteur
Jacques Lagnel, Arnaud A Estoup, Cecile Donnadieu, Celine Lopez-Roques, Maxime Manno, Anne Loiseau, Mathieu Gautier
article
Montpellier Omics days 2018, Université de Montpellier (UM). Montpellier, FRA., Feb 2018, Montpellier, France
resume
La technologie de séquençage long fragments d'Oxford NanoPore (ONT) est basée sur le séquençage des acides nucléiques via le passage de molécules uniques au travers de nano pores protéiques et permet l’obtention de séquences de plusieurs dizaines de kilobases. Elle a très récemment connu une phase exponentielle de développement et son champ d'application s’est élargi à de nombreux domaines de la génomique et notamment l’assemblage de génome de novo. La coccinelle H. axyridis originaire d'Asie, a été introduite pour être utilisée comme agent de lutte biologique mais a établi des populations invasives en Amérique, en Europe et Afrique du Sud. H. axyridis est devenue une espèce modèle pour l’exploration du déterminisme génétique des caractères impliqués dans le succès invasif de certaines populations. D’autre part, avec plus de 200 morphes de couleur d'élytres contrôlés par un seul locus multi-alléliques, H. axyridis est également une espèce emblématique pour l’étude du déterminisme génétique du polymorphisme de couleur. Néanmoins, mener à bien la caractérisation de l’architecture génétique de ces différents caractères d’intérêt nécessite de disposer de ressources génomiques riches et notamment d’assemblages de génome de bonne qualité (la taille haploïde du génome de H. axyrridis étant estimée à 400 Mb). Suite aux résultats relativement décevants en termes de qualité d’assemblage obtenus à partir de données Illumina classiques (séquençage de banques en PE et MP), nous avons généré des séquences longues issues de la technologie ONT (4 flowcells MinIon, 65X, GeT-PlaGe, INRA-Genotoul, France). Un assemblage de novo a été réalisé en combinant ces longs reads ONT avec des reads courts d’Illumina. Plus précisément, le pipeline d'assemblage comprenait une correction des reads initiaux avec le programme bioinformatique Canu suivi d’un assemblage avec SMARTdenovo. L’assemblage ainsi généré présentant un taux riche en délétions d'homopolymères, une étape de polissage a été réalisée avec des lectures Illumina (banques PE et MP). In fine, la contiguïté de notre assemblage est 200 fois supérieure à notre assemblage initial. La moitié du génome obtenu est contenue dans des séquences génomiques de plus de 1,4 Mb avec une complétude évaluée par l’alignement de gènes orthologues conservés (BUSCO) de plus de 95.5%. Enfin, un contig de 1.3Mb nous a permis une résolution moléculaire fine d’une région du génome de 200 kb caractérisée par un grand nombre de SNPs signant des variations moléculaires importantes spécifiques des différents morphes de couleur d’H. axyridis. L’utilisation de la technologie ONT pour l’assemblage de novo de notre espèce non-modèle s’est ainsi révélée d’un excellent rapport qualité/prix.
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titre
VARIATION GENETIQUE ET POTENTIEL D’AMELIORATION DE LA QUALITE NUTRITIONNELLE DES LEGUMES
auteur
Mathilde Causse
article
Association des Sélectionneurs Francais, Feb 2018, Paris, France
resume
Les espèces potagères représentent un groupe d’espèces très hétérogènes, présentant des compositions en phytonutriments très variées. Leur consommation est reconnue comme favorable à l’équilibre nutritionnel et la santé. De nombreuses sources de variation des teneurs en phytonutrients existent (d’ordre physiologique, génétique ou liées aux conditions de production, de récolte, de conservation et de consommation), mais la variation génétique en est souvent la principale. La sélection directe a surtout porté sur l’élimination de composés antinutritionnels ou défavorables plutot que sur l’améliration de teneurs en composés spécifiques. Avec l’avènement des marqueurs moléculaires et de la génomique, les voies de synthèse de la plupart des composés importants ont été disséquées, notamment chez la tomate et de nombreux QTL cartographiés. Quelques gènes ont pu être caractérisés. Le recours aux biotechnologies pour la biofortification a également été envisagé.
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https://hal.inrae.fr/hal-03562894/file/ASF2018_Causse.pdf BibTex
titre
Infrared spectroscopy as a rapid tool to assess apricot fruit quality: comparison of two strategies for a model establishment
auteur
Sylvie Bureau, Catherine M.G.C. Renard, Z. Fakhfackh, Jean Marc Audergon
article
16. International Symposium on Apricot Breeding and Culture, International Society for Horticultural Science (ISHS). INT., 2018, Shenyang, China. pp.5, ⟨10.17660/ActaHortic.2018.1214.24⟩
resume
Infrared spectroscopy is known as a rapid tool to study apricot fruit quality, non-destructively by near-infrared ( NIR, 800-2500 nm) range and destructively on fruit puree by mid-infrared ( MIR, 4000-650 cm(-1)) range. These techniques appear suitable for the determination of soluble solids content ( SSC) and titratable acidity ( TA). However, a major drawback is the need to repeatedly establish calibration equations, which can depend on cultivar, year, etc. The objective here was to evaluate the robustness of NIR and MIR techniques and to determine the best strategy: is it more efficient to build models every year or to build global models combining all years? Eight apricot cultivars, representative of the known apricot diversity for quality traits, have been analysed between 2005 and 2011. The most efficient strategy ( i. e., reliable calibration with a minimum of calibration) was a multi-year model, integrating a limited amount of fruit per year but incremented each year.
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Poster communications

titre
Projets IVD INRA-AgriObtentions variétales pour les légumineuses à graines
auteur
Nathalie Moutier, Mathieu Floriot, Eric Hanocq, Pascal Marget, Rémy Cailliatte, Isabelle Lejeune-Henaut
article
RFL2 Rencontres Francophones Légumineuses, Oct 2018, Toulouse, France. , 2018
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titre
eIF4E-mediated resistance to viruses: from natural variation to synthetic alleles
auteur
Jean-Luc Gallois, Anna Bastet, Nathalie Giovinazzo, Camille Gauffier, Christophe Robaglia
article
International Conference on Arabidopsis Research ICAR 2018, Jun 2018, TURKU, Finland. , 2018
resume
eIF4E translation initiation factors have emerged as major susceptibility factors for RNA viruses. Natural eIF4E-based resistance alleles are found in many species and are mostly variants that maintain the translation function of the protein. eIF4E genes represent major targets for engineering viral resistance, and gene-editing technologies can be used to make up for the lack of natural resistance alleles in some crops, often by knocking out eIF4E susceptibility factors. However, we report here how redundancy among eIF4E genes can restrict the efficient use of knockout alleles in breeding. We therefore discuss how gene-editing technologies can be used to design de novo functional alleles, using knowledge about the natural evolution of eIF4E genes in different species, to drive resistance to viruses without affecting plant physiology. As a proof of concept, we show in Arabidopsis how such trans-species synthetic gene design allows resistance pyramiding and broad spectrum engineering of virus resistance in plants at no yield loss.
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https://hal.inrae.fr/hal-02734736/file/Gallois%20ICAR%202018_1.pdf BibTex
titre
Genome-wide association study of partial resistance to bacterial canker of apricot
auteur
Mariem Omrani, Guillaume Roch, Alain Blanc, Cindy E. Morris, Jean Marc Audergon
article
PAG XXVI - Plant and Animal Genome Conference, Jan 2018, San Diego, United States. 2018
resume
Apricot, a highly valuable crop is threatened by the growing importance of bacterial canker caused by Pseudomonas syringae. Among the key factors able to control the disease, genetic improvement is a promising measure. The variability of susceptibility on branches and the characterization of genetic determinants through a genome wide association study were thus investigated on a core-collection. 73 accessions were annually inoculated in the orchard with an aggressive strain of the bacterium for 4 years. Phenotypic data about the length of both external canker (lgc) and superficial browning (bs) of tissues were collected. The analysis displayed a highly environmental-dependent genetic variation with broad-sense heritabilities of lgc and bs reaching respectively 59% and 78% for the most severe year. Considering the two variance-maximizing years, genetic (G) and genetic x year (GxY) BLUP were predicted for each variable using a linear mixed model. Association analysis were performed with a 63,236 SNP set through both a multi-variate (GEMMA) and a multi-locus genome-wide analysis. By exploiting the between-years (multi-locus model on G and GxY terms) and between-phenotypes (multivariate model on lgc-bs G terms) correlations, 11 significant associations have been detected. Among them, two SNP impacting both lgc and bs expressions over the two studied years and explaining 41% and 31% of the total phenotypic variance were identified on chromosomes 5 and 6. A long-range linkage disequilibrium had been noticed between these two markers suggesting a co-selection effect. The associated SNP reported from this work will open up new opportunities for a Marker-assisted selection strategy.
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Reports

titre
Compte rendu de fin de contrat de recherche, Contrat LactuMel CTPS-CASDAR C-2013-02 : Recherche de résistance aux nématodes à galles chez la laitue
auteur
Brigitte Maisonneuve
article
[Contrat] Gautier semences; Rijk Zwaan; Association Provençale de Recherche et d'Expérimentation Légumière (APREL); Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL); Groupe de Recherche en Agriculture Biologique (GRAB). 2018
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Preprints, Working Papers, ...

titre
Characterization of the early response of Arabidopsis thaliana to Dickeya dadantii infection using expression profiling.
auteur
Frédérique van Gijsegem, Frederique Bitton, Anne-Laure Laborie, Yvan Kraepiel, Jacques Pédron
article
2018
resume
To draw a global view of plant responses to interactions with the phytopathogenic enterobacterale Dickeya dadantii, a causal agent of soft rot diseases on many plant species, we analysed the early Arabidopsis responses to D. dadantii infection. We performed a genome-wide analysis of the Arabidopsis thaliana transcriptome during D. dadantii infection and conducted a genetic study of identified responses. A limited set of genes related to plant defence or interactions with the environment were induced at an early stage of infection, with an over-representation of genes involved in both the metabolism of indole glucosinolates (IGs) and the jasmonate (JA) defence pathway. Bacterial type I and type II secretion systems are required to trigger the induction of IG and JA-related genes while the type III secretion system appears to partially inhibit these defence pathways. Using Arabidopsis mutants impaired in JA biosynthesis or perception, we showed that induction of some IG metabolism genes was COI1-dependent but, surprisingly, JA-independent. Moreover, characterisation of D. dadantii disease progression in Arabidopsis mutants impaired in JA or IG pathways showed that JA triggers an efficient plant defence response that does not involve IGs. The induction of the IG pathway by bacterial pathogens has been reported several times in vitro. This study shows for the first time, that this induction does indeed occur in planta, but also that this line of defence is ineffective against D. dadantii infection, in contrast to its role to counteract herbivorous or fungal pathogen attacks.
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2017

Conference papers

titre
Contournement de la résistance Vat : un peu, beaucoup, pas du tout
auteur
Nathalie Boissot, Pascale Mistral, Virginie Chareyron, Alexandra Schoeny, Hervé Lecoq, Pauline Millot, Flavie Vanlerberghe-Masutti
article
10. journées réseau Biologie et Adaptation des Pucerons et Organismes Associés (BAPOA), Nov 2017, Colmar, France
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titre
Résistance aux pucerons chez le melon et durabilité
auteur
Nathalie Boissot, Alexandra Schoeny, Cecile Desbiez, Frédéric Fabre, Flavie Vanlerberghe-Masutti
article
10. Rencontres du GIS PICLég, Groupement d'Intérêt Scientifique pour la Production Intégrée en Cultures légumières (Gis Piclèg). FRA., Nov 2017, Marseille, France
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titre
Characterization of the RMja resistance gene to root-knot nematodes from the Alnem almond rootstocK
auteur
Henri Duval
article
7. ISHS symposium on Almond, International Society for Horticultural Science (ISHS). INT., Nov 2017, Adélaïde, Australia
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titre
Projet LactuMel, Recherche de résistance aux nématodes à galles chez la laitue
auteur
Brigitte Maisonneuve
article
Restitution de Projets CTPS (CASDAR semences), Comité Technique Permanent de la Sélection (CTPS). FRA. Institut National de la Recherche Agronomique (INRA), FRA., Oct 2017, Paris, France
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titre
Evaluer la sélection génomique au sein de croisements bi-parentaux d’espèces pérennes fruitières génotypés par séquençage : point d’étape du projet FruitSelGen
auteur
Timothée Flutre, Amandine Launay, Caroline Denance, Helene Muranty, Jean Marc Audergon, Carole Confolent, Patrick Lambert, Bénédicte Quilot-Turion, Pierre-François Bert, Elisa Marguerit-Jacob, Elisabeth Dirlewanger, José Quero-Garcia, Stephane Decroocq, Véronique Decroocq, Gisele Butterlin, Eric Duchêne, Evelyne Costes, Jean-Jacques Kelner, Benoit Pallas, Pierre Mournet
article
Séminaire SelGen 2017 : "La sélection génomique, bilan et perspectives", Sep 2017, Paris, France. pp.1
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https://hal.science/hal-01819030/file/2017-09_TFlutre_s%C3%A9minaire-Selgen-Paris_poster-FruitSelGen_1.pdf BibTex
titre
InnoVarTom. Dégradation de la qualité gustative des tomates : un problème de réputation collective ?
auteur
Olivier Bonroy, Mathilde Causse, Aline Fugeray-Scarbel
article
Séminaire SelGen 2017 : "La sélection génomique, bilan et perspectives", Sep 2017, Clichy, France. 1 p
resume
La sélection génomique pourrait permettre de mieux sélectionner les tomates sur le critère de la qualité gustative. Or actuellement, quand une innovation « gustative » (nouvelle typologie de tomate orientée goût) est mise sur le marché, elle perd au fil du temps sa qualité. L’objectif du projet InnoVarTom est de déterminer, à partir d’entretiens semi-directifs, si cette dégradation de la qualité gustative des tomates peut être liée à un problème de détérioration de la réputation collective dans la filière. Nous montrons que les conditions de dégradation de la réputation collective se vérifient effectivement dans la filière tomate.
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https://hal.science/hal-01595680/file/Poster-Innovartom-Bonroy2017_1.pdf BibTex
titre
Variation in phenylpropanoid intermediates associated with adaptation to abiotic stress in the perennial tree species Prunus persica and P. armeniaca
auteur
A. O. Conrad, T. Zhebentyayeva, M. Staton, Jean Marc Audergon, Véronique Decroocq, Z. Liu, C. D. Dardick, C. D. Nelson, A. Abbott
article
Annual Meeting the American-Phytopathological-Society (APS), The American Phytopathological Society (APS). USA., Aug 2017, San Antonio, United States. pp.1
resume
Phenological traits, e.g. dormancy, are mechanisms by which perennial tree species have adapted to stressful conditions that are unfavorable for growth. While there are many mechanisms by which trees adapt and respond to stress, the phenylpropanoid pathway in particular is known to be associated with tree stress response. In this study, Prunus persica (peach) and P. armeniaca (apricot) were used as models to explore the relationship between phenylpropanoids and adaptive trait variation. Concentrations of eight phenylpropanoid intermediates were measured throughout dormancy in floral buds of trees that differed genotypically and phenotypically in chill requirement (peach) and bloom date (apricot). There was a significant interaction effect of time and genotype on the concentration of each of the compounds (mixed ANOVA, P < 0.05). Moreover, concentrations of the flavonoid aglycones, apigenin, kaempferol, and quercetin, dropped precipitously in high chill peach and late flowering apricot, around the endodormancy-ecodormancy transition and at the time when buds began to show signs of blooming (e.g. presence of sepals and petals). These results suggest a potential role of phenylpropanoid intermediates in mediating the resumption of growth and development following winter dormancy. A better understanding of tree responses to stress, will be critical for tree improvement programs aimed at identifying and breeding trees more suitable for rapidly changing environments.
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Deciphering regulation levels of sugar metabolism during peach fruit development through metabolic, enzymatic, genetic analyses and kinetic modeling
auteur
Bénédicte Quilot-Turion, Elsa Desnoues, Valentina Baldazzi, Michel Génard, Veronique Signoret, Patrick Lambert, Carole Confolent, Laure Heurtevin
article
ISHS 9. International Peach Symposium, International Society for Horticultural Science (ISHS). INT., Jul 2017, Bucarest, Romania
resume
Deciphering regulation levels of sugar metabolism during peach fruit development through metabolic, enzymatic, genetic analyses and kinetic modeling. ISHS 9. International Peach Symposium
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titre
Advances in pyramiding resistance genes to root-knot nematodes in Prunus rootstocks
auteur
Henri Duval, Cyril van Ghelder, Guillaume Roch, Daniel Esmenjaud
article
9. International Peach symposiumHS, International Society for Horticultural Science (ISHS). INT., Jul 2017, Bucarest, Romania
resume
Advances in pyramiding resistance genes to root-knot nematodes in Prunus rootstocks. 9. International Peach symposiumHS
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titre
Differential gene expression analysis in the threatened sponge Spongia officinalis following exposure to different temperature conditions.
auteur
V Koutsouveli, Tereza Manousaki, Jacques Lagnel, S Kollias, Costas Tsigenopoulos, C Arvanitidis, C Dounas, Antonios Magoulas, Ana Riesgo, Thanos Dailianis
article
10. World Sponge Conference, National University of Ireland Galway. IRL., Jun 2017, Galway, Ireland
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Genetic diversity and architecture of gene expression in response to water deficit in tomato
auteur
Elise Albert, Christopher Sauvage, Jean-Paul Bouchet, Frederique Bitton, Matthieu Beukers, Renaud Duboscq, Yolande Carretero, Mathilde Causse
article
Improving Agriculture through Genomics CROPS 2017, Jun 2017, Huntsville, United States
resume
Tomato is both a model species thanks to the large genetic and genomic resources available and an important crop. Tomato fruit quality is characterized by a large number of components influenced by the genotypes and the environment, notably water status. In order to unravel the genetic control of these components, quantitative trait loci for transcript amount (eQTL), metabolic and phenotypic traits (phQTL) have been mapped under control and water deficit conditions. We used a RIL population derived from an intraspecific cross between a cherry tomato line and a large fruited line. The genomes of both parents were sequenced and differ by more than 2 million SNPs. RNAseq of parental lines revealed a number of functions affected by water stress, but also some major differences according to the parents. On the RIL population we harvested fruit and leaf samples and performed eQTL mapping for 270 differentially expressed genes using the fluidigm technology. Several eQTL were mapped, some with strong effects, a majority of which acting as cis eQTL, and clusters of eQTL and pQTL were demonstrated. Analysis of the transcriptome of the F1 hybrid allowed the quantification of allele specific expression and identification of almost 2000 cis-regulated genes and 1,450 trans regulated genes. Contrary to trans regulatory divergences, cis regulatory divergences were strongly conserved between organs and between watering regimes. Results will be discussed in regard to the gene functions and co-localisations with metabolite or phenotype pQTLs.
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https://hal.science/hal-01607155/file/CROPS_abstract-eQTL_1.pdf https://hal.science/hal-01607155/file/CROPS_abstract-eQTL_2.docx BibTex
titre
Allier géostatistique et algorithme EM pour cartographier la date d’apparition des hommes
auteur
Edith Gabriel, Frédérik Saltré, Joel Chadoeuf
article
49èmes journées de statistique, May 2017, Avignon, France
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titre
Des résistances aux nématodes à galles du genre Meloidogyne dans le genre Lactuca : espoir pour la sélection de la laitue
auteur
Brigitte Maisonneuve
article
Rencontres techniques phytosanitaires SDQSPV/ Ctifl, légumes et fraise, Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL). FRA., Jan 2017, Bellegarde, France
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Integration of QTL, eQTL and allele specific expression to unravel genotype by watering regime interaction in cultivated tomato
auteur
Elise Albert, Christopher Sauvage, Jean-Paul Bouchet, Frederique Bitton, Matthieu Beukers, Yolande Carretero, Mathilde Causse
article
24. Plant and Animal Genome Conference (PAG), Plant & Animal Genome (PAG). USA., Jan 2017, San Diego, United States
resume
Tomato is both a model species and important crop. Tomato fruit quality is characterized by a large number of components influenced by the genotypes and the environment, notably water status. In order to unravel the genetic control of these components, quantitative trait loci for metabolic and phenotypic traits (pQTL) have been mapped under control and drought conditions. We used a RIL population derived from an intraspecific cross between a cherry tomato line and a large fruited line. The genomes of both parents were sequenced and differ by more than 2 million SNPs. RNAseq of parental lines revealed a number of functions affected by water stress, but also some major differences according to the parents. On the RIL population we harvested fruit and leaf samples and performed eQTL mapping for 100 differentially expressed genes using the fluidigm technology. Several eQTL were mapped, some with strong effects, a majority of which acting as cis eQTL, and clusters of eQTL and pQTL were demonstrated. Analysis of the transcriptome of the F1 hybrid allowed the quantification of allele specific expression and identification of other strong effect cis eQTL. Results will be discussed in regard to the gene functions and co-localisations with metabolite or phenotype pQTLs.
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titre
Impact of selection, genetic drift and viral accumulation on the evolution of a plant RNA virus
auteur
Lucie Tamisier, Gregory Girardot, Ghislaine Nemouchi, Marion Szadkowski, Alain Palloix, Benoît Moury
article
16. Rencontres de Virologie Végétale (RVV 2017), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA., Jan 2017, Aussois, France. 137 p
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https://hal.science/hal-01606407/file/2017_Tamisier_RVV_1.pdf BibTex
titre
A new eIF4E1 allele characterized by RNAseq data mining is associated with resistance to PVY in tomato albeit with a low durability
auteur
Caroline Lebaron, Aurélie Rosado, Christopher Sauvage, Camille Gauffier, Sylvie German-Retana, Benoît Moury, Jean-Luc Gallois
article
16. Rencontres de Virologie Végétale (RVV 2017), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA., Jan 2017, Aussois, France. 137 p
resume
Allele mining on susceptibility factors offers opportunities to find new sources of resistance among crop wild relatives for breeding purposes. As a proof of concept, we used available RNAseq data to investigate polymorphisms among the four tomato genes encoding translation initiation factors (eIF4E1 and eIF4E2, eIFiso4E and the related gene New Cap-binding Protein -nCBP) to look for new potential resistance alleles to potyviruses. By analyzing polymorphism among RNAseq data obtained for 20 tomato accessions, ten belonging to the cultivated type Solanum lycopersicum and 10 belonging to the closest related wild species Solanum pimpinellifolium, we isolated one new eIF4E1 allele, in the Solanum pimpinellifolium LA0411 accession, which encodes a potential new resistance allele, mainly due to a polymorphism associated with an amino acid change within eIF4E1 region II. We confirmed that this new allele, pot1², is indeed associated with resistance to PVY, although with a restricted resistance spectrum and a very low durability potential. This suggests that mutations occurring in eIF4E region II only may not be sufficient to provide efficient and durable resistance in plants. However, our study emphasizes the opportunity brought by RNAseq data to mine for new resistance alleles. Moreover, this approach could be extended to seek for putative new resistance alleles by screening for variant forms of susceptibility genes encoding plant host proteins known to interact with viral proteins.
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Discovering peach QTLs with multiple progeny analysis
auteur
J.R. Hernández Mora, D. Micheletti, Mc.A.M. Bink, W.E. van de Weg, D. Bassi, N. Nazzicari, A. Caprera, M.T. Dettori, S. Micali, Elisabeth Dirlewanger, Patrick Lambert, Thierry Pascal, E. Banchi, M. Troggio, L. Rossini, I. Verde, Bénédicte Quilot-Turion, Francois Laurens, P. Arus, M.J. Aranzana
article
14. EUCARPIA Symposium on Fruit Breeding and Genetics, 2017, Bologne, Italy. ⟨10.17660/ActaHortic.2017.1172.77⟩
resume
Although many peach QTLs responsible for the variability of traits have been identified and published so far, the number of molecular markers currently used in breeding is still limited. One of the reasons is the large QTL intervals produced, in part, by the limited progeny size. Here we report a QTL mapping approach that enlarges the progeny size by analyzing jointly multiple progenies. Our analysis included 1467 individuals from eighteen Prunus progenies, 13 from intra-specific crosses and five inter-specific between peach and closely related species. The progenies were grown in five locations, with no duplication between orchards. Data from phenology, tree, flower and fruit traits, fruit quality and yield measured in different locations and years were subjected to different standardization methods and integrated in a single data file. The populations were genotyped with the 9K SNP Illumina array, which increased considerably the marker density compared to previous studies. The QTL analysis was conducted with FlexQTLTM software. Here we describe and discuss the preliminary QTLs obtained for some of the traits analyzed (maturity date, percentage of red skin color and soluble solid content). The identification of donors of favorable alleles will represent an important tool for marker-assisted breeding. This study has been conducted in the frame of the Fruit Breedomics European project.
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Other publications

titre
Database Of Weeds In Cultivation Fields Of France And Uk, With Ecological And Biogeographical Information
auteur
François Munoz, Guillaume Fried, Laura Armengot, Bérenger Bourgeois, Vincent V. Bretagnolle, Joel J. Chadoeuf, Lucie Mahaut, Christine Plumejeaud-Perreau, Jonathan Storkey, Cyrille Violle, Sabrina Gaba
article
2017, ⟨10.5281/zenodo.1112342⟩
resume
The database includes a list of 1577 weed plant taxa found in cultivated fields of France and UK, along with basic ecological and biogeographical information. The database is a CSV file in which the columns are separated with comma, and the decimal sign is ".". It can be imported in R with the command "tax.discoweed <- read.csv("tax.discoweed_18Dec2017_zenodo.csv", header=T, sep=",", dec=".", stringsAsFactors = F)" Taxonomic information is based on TaxRef v10 (Gargominy et al. 2016), - 'taxref10.CD_REF' = code of the accepted name of the taxon in TaxRef, - 'binome.discoweed' = corresponding latine name, - 'family' = family name (following APG III), - 'taxo' = taxonomic rank of the taxon, either 'binome' (species level) or 'infra' (infraspecific level), - 'binome.discoweed.noinfra' = latine name of the superior taxon at species level (different from 'binome.discoweed' for infrataxa), - 'taxref10.CD_REF.noinfra' = code of the accepted name of the superior taxon at species level. The presence of each taxon in one or several of the following data sources is reported: - Species list from a reference flora (observations in cultivated fields over the long term, without sampling protocol), * 'jauzein' = national and comprehensive flora in France (Jauzein 1995), - Species lists from plot-based inventories in cultivated fields, * 'za' = regional survey in 'Zone Atelier Plaine & Val de Sèvre' in SW France (Gaba et al. 2010), * 'biovigilance' = national survey of cultivated fields in France (Biovigilance, Fried et al. 2008), * 'fse' = Farm Scale Evaluations in England and Scotland, UK (Perry, Rothery, Clark et al., 2003), * 'farmbio' = Farm4Bio survey, farms in south east and south west of England, UK (Holland et al., 2013) - Reference list of segetal species (species specialist of arable fields), * 'cambacedes' = reference list in France (Cambacedes et al. 2002) Life form information is extracted from Julve (2014) and provided in the column 'lifeform'. The classification follows a simplified Raunkiaer classification (therophyte, hemicryptophyte, geophyte, phanerophyte-chamaephyte and liana). Regularly biannual plants are included in hemicryptophytes, while plants that can be both annual and biannual are assigned to therophytes. Biogeographic zones are also extracted from Julve (2014) and provided in the column 'biogeo'. The main categories are 'atlantic', 'circumboreal', 'cosmopolitan, 'Eurasian', 'European', 'holarctic', 'introduced', 'Mediterranean', 'orophyte' and 'subtropical'. In some cases, a precision is included within brackets after the category name. For instance, 'introduced(North America)' indicates that the taxon is introduced from North America. In addition, some taxa are local endemics ('Aquitanian', 'Catalan', 'Corsican', 'corso-sard', 'ligure', 'Provencal'). A single taxon is classified 'arctic-alpine'. Red list status of weed taxa is derived for France and UK: - 'red.FR' is the status following the assessment of the French National Museum of Natural History (2012), - 'red.UK' is based on the Red List of vascular plants of Cheffings and Farrell (2005), last updated in 2006. The categories are coded following the IUCN nomenclature. A habitat index is provided in column 'module', derived from a network-based analysis of plant communities in open herbaceous vegetation in France (Divgrass database, Violle et al. 2015, Carboni et al. 2016). The main habitat categories of weeds are coded following the Divgrass classification, - 1 = Dry calcareous grasslands - 3 = Mesic grasslands - 5 = Ruderal and trampled grasslands - 9 = Mesophilous and nitrophilous fringes (hedgerows, forest edges...) Taxa belonging to other habitats in Divgrass are coded 99, while the taxa absent from Divgrass have a 'NA' value. Two indexes of ecological specialization are provided based on the frequency of weed taxa in different habitats of the Divgrass database. The indexes are network-based metrics proposed by Guimera and Amaral (2005), - c = coefficient of participation, i.e., the propensity of taxa to be present in diverse habitats, from 0 (specialist, present in a single habitat) to 1 (generalist equally represented in all habitats), - z = within-module degree, i.e., a standardized measure of the frequency of a taxon in its habitat; it is negatve when the taxon is less frequent than average in this habitat, and positive otherwise; the index scales as a number of standard deviations from the mean.
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Cépages résistants : la vigne contre-attaque
auteur
Laurent Deliere, Christophe Schneider, Laurent Audeguin, Loïc Le Cunff, Rémy Cailliatte, Emilce Prado, Christine Onimus, Isabelle Demeaux, Soizic Guimier, Frédéric Fabre, François Delmotte
article
2017, pp.34-37
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Manipuler les forces évolutives exercées par les plantes sur les virus pour améliorer la durabilité des gènes de résistance
auteur
Benoît Moury, Julie Quenouille-Lederer, Elsa Rousseau, Lucie Tamisier, Ghislaine Nemouchi, Marion Szadkowski, Gregory Girardot, Pauline Millot, Ludovic Mailleret, Frédéric Grognard, Frédéric Fabre, Alain Palloix
article
2017, pp.63-74
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Poster communications

titre
Study of the graft-compatibility of almond on myrobalan (Prunus cerasifera) rootstocks
auteur
Henri Duval, Patrice Duvivier
article
7. ISHS symposium on Almond, Nov 2017, Adélaïde, Australia. 2017
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PUX10 associates with CDC48A and regulates the dislocation of ubiquitinated oleosins from seed lipid droplets
auteur
Carine Deruyffelaere, Zita Purkrtova, Isabelle Bouchez, Boris Collet, Jean-Luc Cacas, Thierry Chardot, Jean-Luc Gallois, Sabine D’andrea
article
13th GERLI Lipidomics Meeting, Oct 2017, Dijon, France.
resume
In oleaginous seeds, storage lipids are packed in organelles called lipid droplets (LDs) and are degraded post-germinatively to provide carbon and energy for seedling growth. The major LD-associated proteins, called oleosins, form a protective barrier that shields stored lipids from lipases, thus preventing lipolysis. The degradation of this barrier begins just prior lipid degradation in germinating seeds. Oleosin ubiquitination was recently shown to control oleosin degradation in Arabidopsis seedlings1. Ubiquitination of oleosins is surprisingly complex. Three distinct and exclusive motifs can be attached to the major oleosins OLE1 and OLE2 and may channel modified oleosins towards specific degradation pathways according to ubiquitination type. Once ubiquitinated, oleosins are extracted from the LD coat before proteolysis. The molecular mechanism of oleosin extraction is yet unknown. To gain insight to the proteins involved in oleosin extraction, we analyzed proteomic data obtained from LDs isolated in Arabidopsis germinated seeds. We identified a putative ubiquitin-binding protein and an AAA ATPases protein called PUX10 and CDC48A, respectively. To explore their role in LD dynamics, we undertook functional analyses using T-DNA mutants. Because CDC48A-deficient mutants were reported as lethal2, we focused our study on PUX10 mutants. We showed that PUX10 mutation blocks the degradation of all the three types of ubiquitinated oleosins, and prevents the dislocation of ubiquitinated oleosins from LDs, which precedes the degradation. By confocal microscopy and organelle fractionation, we confirmed that PUX10 is an integral LD protein and we identified its membrane-anchoring domain. By microscopy, Y2H, Y3H and BiFC, we demonstrated that PUX10 interacts with ubiquitin and CDC48A through its UBA and UBX domains, respectively. Overall, our data revealed that PUX10 is an adaptor bridging ubiquitinated oleosins and CDC48A, thus allowing the ubiquitin-selective « segregase » activity of CDC48A to separate ubiquitinated proteins from LDs.
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Poster communications
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https://agroparistech.hal.science/hal-04549342/file/20171020%20Carine%20DERUYFFELAERE-GERLI%202017.pdf BibTex
titre
Sélection génomique - Quelles perspectives chez les Prunus ?
auteur
Mariem N'Sibi, Carole Confolent, Patrick Lambert, Sylvie Bureau, Barbara Gouble, Alain Blanc, Guillaume Roch, Mariem Omrani, Timothée Flutre, Christopher Sauvage, Jean Marc Audergon
article
Séminaire SelGen 2017 : "La sélection génomique, bilan et perspectives", Sep 2017, Paris, France. 1 p, 2017
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https://hal.inrae.fr/hal-02789979/file/2017_FruitSelGen-Abricotier_v1_1.pdf BibTex
titre
Are sown flower strips an efficient tool to limit aphid colonization and viral epidemics in melon crops?
auteur
Alexandra Schoeny, Nathalie Boissot, Catherine Wipf-Scheibel, Karine Nozeran, Pascale Mistral, Patrick Gognalons, Pauline Millot, Hervé Lecoq
article
10. Conférence de la Société française de phytopathologie, 12. European foundation for plant pathology conference, May 2017, Malo-les-Bains, France. , 2017, Deepen knowledge in plant pathology for innovative agro-ecology
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https://hal.science/hal-01605708/file/2017_Schoeny_EFPP_Poster_1 https://hal.science/hal-01605708/file/2017_Schoeny_EFPP_Poster2_2.pdf BibTex
titre
A synthetic eIF4E1 resistance allele allows resistance pyramiding and broad resistance spectrum to potyviruses in Arabidopsis thaliana
auteur
Anna Bastet, Baptiste Lederer, Nathalie Giovinazzo, Joan Estevan, Christophe Robaglia, Jean-Luc Gallois
article
16. Rencontres de Virologie Végétale (RVV 2017), Jan 2017, Aussois, France. 2017
resume
Developing genetic resistance is an important alternative to the use of pesticides in order to avoid viral infections in crops. However, this approach can be limited by the lack of natural resistant alleles available for plant breeding. Biotechnologies can supply for this lack by altering susceptibility host factors used by viruses. However, strategies relying on knocking out plant host factors can be undermined by associated developmental default, or gene redundancy (Gauffier et al., 2016). Synthetic biology could offer the opportunity to develop functional synthetic alleles associated with plant resistance at no developmental cost. The translation initiation factors (eIF4E), that are associated with resistance to most single strand positive RNA (including the major group of potyviruses), are the ideal targets to validate such approaches. We focused on the Arabidopsis thaliana eIF4E1 gene that encodes a susceptibility factor to a potyvirus, the Clover yellow vein virus. Artificial resistance alleles were created by directed mutagenesis and used to complement loss-of-function eif4e1 plants for assessing in planta both their functionality and resistance efficiency. By combining these synthetic resistance alleles with others eIF4E factors-mediated resistance, a broad resistance spectrum to potyviruses is expected as well as increased resistance durability. This study will make proof of the feasibility of this approach to obtaining large resistance spectrum in plants with the perspective of extending it to crops.
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Poster communications
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https://hal.science/hal-01594565/file/Bastet_Gallois%20Aussois%202017_1.pdf https://hal.science/hal-01594565/file/Bastet_Gallois%20Aussois%202017_2.docx BibTex
titre
Finding loss-of-susceptibility resistance gene toward tospoviruses in Solanaceae
auteur
Zoé Terret, Grégori Bonnet, Laure Didierlaurent, Jean-Luc Gallois
article
16. Rencontres de Virologie Végétale (RVV 2017), Jan 2017, Aussois, France. 2017
resume
Tospovirus is the only member of the Bunyavirus family which infects plants (the other members infecting animals and humans). Tomato spotted wilt virus (TSWV), the most famous tospovirus, is a major pathogen for tomato and pepper cultures. Because there is no chemical treatment against viruses, the only way to fight them is to develop genetic resistance. Genetic resistances to tospoviruses are available, but are largely overcome. Therefore, there is an urgent need to characterize new sources of resistance. The aim of my PhD is to find new genetic resistances based on susceptibility factors, which are proteins required by the virus to accomplish its cycle and infect the plant. As a first step, I will develop a 3D model of the TSWV RNA-dependent RNA polymerase (RdRp), a key viral protein for its infectious cycle. Based on this model, a part of the RdRp protein will be selected as bait in order to identify plant protein interactors by yeast-two hybrid screening. Finally, I will search for new tomato alleles encoding variant forms of those tomato susceptibility factors. If those variants impair the interaction between the plant factor and the viral protein, those alleles are very likely to be associated with the crop resistance to the pathogen.
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Poster communications
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https://hal.science/hal-01604721/file/Terret_Gallois%20Aussois%202017%20Poster_1.pdf https://hal.science/hal-01604721/file/Terret_Gallois%20Aussois%202017%20Poster_2.docx BibTex
titre
Exploring plant tolerance to viruses as a sustainable means of disease control
auteur
Judith Hirsch, Gregory Girardot, Sandra Martin, Jawad Aarrouf, Alain Palloix, Benoît Moury
article
16. Rencontres de Virologie Végétale (RVV 2017), Jan 2017, Aussois, France. , 137 p., 2017, 16ièmes Rencontres de Virologie Végétale - 15 -19 janvier 2017 - Aussois - France
resume
Exploring plant tolerance to viruses as a sustainable means of disease control. 16. Rencontres de Virologie Végétale (RVV 2017)
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Poster communications
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https://hal.science/hal-01605629/file/2017_Hirsch_RVV_1.pdf BibTex

Videos

titre
Restitution du programme LactuMel
auteur
Brigitte Maisonneuve
article
2017
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Videos
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https://hal.inrae.fr/hal-02786257/file/11_Lactumel_Maisonneuve_V02.mp4 BibTex

2016

Conference papers

titre
Durabilité des résistances médiées par Vat
auteur
Alexandra Schoeny, Nathalie Boissot
article
Rencontre Ecophyto Melon Sud-Est, Dec 2016, Avignon, France. 15 p
resume
Durabilité des résistances médiées par Vat. Rencontre Ecophyto Melon Sud-Est
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titre
Projet LactuMel (2014-2016): Recherche de résistance aux nématodes à galles chez la laitue
auteur
Brigitte Maisonneuve
article
Rencontres du GIS PIClég, Groupement d'Intérêt Scientifique pour la Production Intégrée en Cultures légumières (Gis Piclèg). FRA., Nov 2016, Nantes, France
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titre
Screening a priori durable resistance in tomato to Phytophthora infestans using its pathogenicity effectors
auteur
Emmanuel Szadkowski, Gaëtan Maillot, Alexandre Bachellez, Dorine Achard, Anne Massire, Bernard Caromel, Véronique Lefebvre
article
Effectome meeting 2016, Nov 2016, Lauret, France
resume
Screening a priori durable resistance in tomato to Phytophthora infestans using its pathogenicity effectors. Effectome meeting 2016
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titre
Les porte-greffes de l'amandier
auteur
Henri Duval
article
1. journée de l'amande, Nov 2016, Plan d'Orgon (13), France
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Conference papers
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https://hal.inrae.fr/hal-02743311/file/6_porte-greffes2016_1.pdf https://hal.inrae.fr/hal-02743311/file/Referentiel_amande_2016_2017_2.pdf BibTex
titre
Effet sur la démographie et la composition génétique des populations de pucerons dans les cultures de melons résistants vs sensibles
auteur
Nathalie Boissot
article
3. Meeting Resistance, Nov 2016, Lauret, France
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absent
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titre
La recherche et le développement : une nouvelle organisation au service de nos producteurs. Enjeux et questions posées à la filière fruits à noyau
auteur
Jean Marc Audergon
article
Séminaire AOP Pêches et Abricots de France, Nov 2016, Baillargues, France. 14 p
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Conference papers
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https://hal.inrae.fr/hal-02793499/file/Audergon%20S%C3%A9minaire%20AOP%202016_1.pptx https://hal.inrae.fr/hal-02793499/file/Audergon%20S%C3%A9minaire%20AOP%202016_2.pptx BibTex
titre
ARDU. ARboriculture Durable: approches interdisciplinaires
auteur
Claire Lamine, Sylvaine Simon, Jean Marc Audergon
article
Métaprogramme INRA Gestion durable de la santé des cultures, Oct 2016, Paris, France. 21 p
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Conference papers
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https://hal.inrae.fr/hal-02801861/file/2016_Lamine_ARDUSmash_1.pdf BibTex
titre
A systems biology study in tomato fruit reveals signaling from the ascorbate pool and links with translation and the heat-shock response
auteur
Rebecca Stevens, Pierre Baldet, Jean-Paul Bouchet, Mathilde Causse
article
Annual Conference COST FA1106 “Quality Fruit”, Oct 2016, Porto, Portugal. 36 p
resume
Plants with lowered activity of enzymes catalyzing the final step of ascorbate synthesis or the equilibrium between reduced and oxidized forms have highly pleiotropic phenotypes suggesting regulatory roles for ascorbate and its oxidized forms. Network analysis of the transcriptome, proteome and key metabolites of RNAi lines for ascorbate oxidase, monodehydroascorbate reductase and galactonolactone dehydrogenase has been carried out in orange fruit pericarp of tomato (Solanum lycopersicum). We show a transcriptional switch-type response with expression profiles in ascorbate oxidase lines being inversed compared to the monodehydroascorbate reductase and galactonolactone dehydrogenase lines. Differentially expressed genes are linked to ribosome biogenesis and translation. The inversion between the transcriptome signatures is not specific to tomato fruit as it is conserved in Arabidopsis. The transcriptome response is not correlated with accumulated proteins which, with the metabolites, are correlated to the activity of the ascorbate redox enzymes. Differentially accumulated proteins include metacaspase, protein disulphide isomerase, chaperone DnaK and carbonic anhydrase and the metabolites chlorogenic acid, dehydroascorbate and alanine. Hub genes are related to signaling, the heat-shock response and ribosome biogenesis. This study in a non-photosynthetic tissue reveals signaling from the ascorbate pool related to the heat-shock response, translation and protein synthesis.
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titre
Analyse agroclimatique du contexte de la floraison de l'abricotier (Var Bergeron). Quelques éléments d'analyse à partir d'un retour sur plusieurs décennies
auteur
Patrick Bertuzzi, Jean Marc Audergon, I. García de Cortázar-Atauri
article
Présentation Sefra, Sep 2016, Gotheron, France. 28 p
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Conference papers
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https://hal.inrae.fr/hal-02794141/file/Audergon%20Presentation_SEFRA%202016_1.pdf BibTex
titre
A compartmental epidemiological model for brown rot spreading in stone fruits orchard
auteur
Danièle Bevacqua, Michel Génard, Bénédicte Quilot-Turion, Leandro Oliveira Lino, Vincent Mercier, Françoise Lescourret
article
Hortimodel2016, 19-22 Septembre, Avignon, Sep 2016, Avignon, France. pp.1-40
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Conference papers
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https://hal.inrae.fr/hal-03587185/file/HORTIMODEL_AbstractBook.pdf BibTex
titre
A model-based approach for peach fruit transpiration process and its genetic variability
auteur
M. Memmah, Bénédicte Quilot-Turion, Michel Génard, Imen Soula
article
Hortimodel2016, 19-22 Septembre, Avignon, Sep 2016, Avignon, France. pp.1-40
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Conference papers
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https://hal.inrae.fr/hal-03587187/file/HORTIMODEL_AbstractBook.pdf BibTex
titre
A kinetic model of sugar metabolism in peach fruit allows the exploration of genetic variability
auteur
Elsa Desnoues, Bénédicte Quilot-Turion, Michel Génard, Valentina Baldazzi
article
Hortimodel2016, 19-22 Septembre, Avignon, Sep 2016, Avignon, France. pp.1-39
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Conference papers
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https://hal.inrae.fr/hal-03587210/file/HORTIMODEL_AbstractBook.pdf BibTex
titre
Quantitative trait loci in pepper genome control the effective population size of two RNA viruses at inoculation
auteur
Lucie Tamisier, Elsa Rousseau, Sebastien Barraillé, Ghislaine Nemouchi, Marion Szadkowski, Ludovic Mailleret, Frédéric Grognard, Frédéric Fabre, Benoît Moury, Alain Palloix
article
16. Eucarpia Capsicum and Eggplant meeting, Szent István University. Gödöllo, HUN., Sep 2016, Kecskemét, Hungary. 591 p
resume
Infection of plants by viruses is a complex process that involves several steps: inoculation into plant cells, replication in inoculated cells, cell-to-cell movement during leaf colonization and long-distance movement during systemic infection. The success of the different steps is conditioned by the effective viral population size (Ne) defined as the number of individuals that pass their genes to the next generation. During the infection cycle, the virus population will endure several bottlenecks leading to drastic reductions in Ne and to the random loss of sorne virus variants. If strong enough, these bottlenecks could act against selection by eliminating the fittest variants. Therefore, a better understanding of how plant affects Ne rnay contribute to the developrnent of durable virus-resistant cultivars. We aimed to (i) identify plant genetic factors that control Ne at the inoculation step, (ii) understand the mechanisms used by the plant to control Ne and (iii) compare these genetic factors with other genes controlling virus life cycle and plant resistance durability. The virus effective population size was measured in a segregating population of 152 doubled-haploid lines of Capsicum annuum. Plants were inoculated mechanically either with a Patata virus Y (PVY) construct expressing the green fluorescent protein (OFP), or a necrotic variant of Cucumber mosaic virus (CMV), the CMV-N strain of Fulton. Ne was assessed by counting the number ofprimary infection foci observed on inoculated cotyledons under UV light for PVY -OFP or the number of necrotic local lesionsobserved on inoculated leaves for CMY-N. The numbers of primary infection foci and locallesions were correlated arnong the doubled-haploid lines (r=0.57) and showed a high heritability (h2=0.93 and 0.98 for PVY and CMV, respectively). The effective population size of the two viruses was shown to be controlled by botb common quantitative trait loci (QTLs) and virus-specifie QTLs, indicating the contribution ofboth general and specifie mechanisms. The PVY-specific QTL colocalizes with a QTL that had previously been shown to be involved in PVY accumulation and capacity to break amajor-effect resistance gene down.
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Conference papers
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https://hal.inrae.fr/hal-02738767/file/Tamisier_Palloix%2016th%20eucarpia%202016%20169-176_1.pdf BibTex
titre
Plant genetic background increasing the efficiency and durability of major resistance genes to root knot nematodes can be resolved into a few resistance QTLs
auteur
Arnaud Barbary, Caroline Djian-Caporalino, Philippe Castagnone-Sereno, Nathalie Marteu, Ariane Fazari, Bernard Caromel, Alain Palloix
article
16. Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary. 4 p
resume
With the banning of most chemical nematicides, the control of root knot nematodes (RKNs) in vegetable crops is mainly based on the deployment of single-major resistance genes (R-genes). However, these genes are rare and their efficiency is threatened by RKNs capacities of adaptation. ln pepper, several dominant R-genes are efficient against RKNs, but their efficiency and durability were shown to be increased in partially resistant genetic background. A QTL analysis was performed in such a genetic background, using a F2:3population from the cross between Yolo Wonder, a partially resistant to resistant accession depending on RKN species, and Doux Long des Landes, a susceptible one. The genetic linkage map was constructed from 130 F2 individuals and the 130 F3 families were tested forresistance to the three main RKNs species, M incognita, M arenaria and Mjavanica. Four new major QTLs were mapped into two clusters. The cluster on chromosome Pl includes three tightly linked QTLs with specifie effects against each RKN species. The fourth QTL, specifie of resistance to M javanica, mapped on the pepper chromosome P9, which is known to carry multiple NBS-LRR repeats with major resistance genes to nematodes and other pathogens. The newly discovered cluster on chromosome Pl, displays a broad spectrum of action with major additive effects on resistance. Therefore, it provides innovative potential for breeding new cultivars or rootstocks combining quantitative resistance and major resistance genes and increasing the efficiency as weil as the durability ofRKNs genetic control.
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Conference papers
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https://hal.inrae.fr/hal-02792960/file/Caromel-Palloix%2016th%20eucarpia%202016%20poster%20523-527_1.pdf BibTex
titre
Eggplant resistance to bacterial wilt and to Fusarium wilt: Is there a link?
auteur
Marie-Christine Daunay, Jean Winoc Hennart, Sylvia Salgon, Jacques Dintinger
article
16. Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary. 14 p
resume
INRA URI 052 maintains a germplasm collection of S. melongena and related species. The accessions of this collection that are described in the literature as resistant to Ralstonia solanacearum, agent responsible ofbacterial wilt, were screened at plantlet stage against Fusarium oxysporum f.sp. melongenae on the basis of the number ofleaves wilted and vessels browning. Results show that the panel of accessions tested display phenotypes ranging from fully resistant to fully susceptible to Fusarium wilt, with ail intermediate resistance levels. Results are discussed on the basis ofthe complexity ofresistance evaluation and of what is known in tomato about a link between resistances to both vascular diseases.
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Conference papers
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https://hal.inrae.fr/hal-02801124/file/Daunay%2016th%20eucarpia%202016%2099-111_1.pdf BibTex
titre
Screening of solanaceous wild relatives for graft affinity with eggplant (Solanum melongena L.)
auteur
François Villeneuve, François Latour, Théophile Théry, Patricia Erard, Christine Fournier, Marie-Christine Daunay
article
16. Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary. 9 p
resume
Eggplant (Solanum melongena L.), an important solanaceous crop in tbe world, is susceptible to soilbome bio-agressors, the main of which, in temperate areas, being Verticillium wilt (Verticillium sp.) and root knot nematodes (Meloidogyne spp.). Grafting on resistant rootstock is an alternative rnethod to soil disinfection. In France, the generalization of grafting spread over the 2000's. After several years of utilization of resistant tornato rootstocks for eggplant production, and to a lesser extent, of Solanum torvum rootstocks, damaging wilt-like-symptoms are often observed by growers. Renee it is necessary to identify alternative rootstocks, able to sustain the soil pathogenic cornplex by a combination of vigor and genetic resistance. However, graft affinity between potential rootstocks and eggplant (scion) is the fust step to investigate. Solanaceae family offers a wide choice of candidate rootstock species. The objective ofthis research was to identify solanaceous species baving a good graft affinity with eggplant, Screening of a wide set of genetic resources was carried out at the Centre Technique Interprofessionnel des Fruits et Légumes -Ctifl- (Lanxade and Balandran center) in collaboration with INRA. Experirnents were carried out during a five years period (2011-2015). Several So/anum species displayed a good graft affinity with eggplant and are candidates for further agronomie evaluation.
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Conference papers
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https://hal.inrae.fr/hal-02794118/file/Daunay%2016th%20eucarpia%202016%20152-160_1.pdf BibTex
titre
Genetic mapping of broad-spectrum QTLs and strain-specific major QTL for resistance to Ralstonia solanacearum in eggplant using GBS
auteur
Sylvia Salgon, Cyril Jourda, Christopher Sauvage, Marie-Christine Brand-Daunay, Emmanuel Wicker, Jacques Dintinger
article
16. Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary
resume
Genetic mapping of broad-spectrum QTLs and strain-specific major QTL for resistance to [i]Ralstonia solanacearum[/i] in eggplant using GBS. 16. Eucarpia Capsicum and Eggplant Meeting
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Conference papers
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https://hal.science/hal-01512188/file/Sauvage_Daunay%2016th%20eucarpia%202016%20246_1.pdf BibTex
titre
Greenhouse eggplant production in France: Success and failures of grafting for controlling Verticillium sp.
auteur
François Villeneuve, François Latour, Théophile Théry, Christian Steinberg, Véronique Edel-Hermann, Marie-Christine Brand-Daunay
article
16. Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary. 6 p
resume
After many years of utilisation of grafting on Verticillium wilt resistant rootstocks, mainly tomato rootstocks, growers observe new wilt like symptoms on their grafted eggplants. Accordingly, Ctifl, in close collaboration with lNRA, regional extension services, and seed companies, set up a research designed to get a general picture of the various soil borne phytosanitary problems, and to identify their causees). The princip le of the study was to monitor pathogen distribution changes in eggplant crops in the two main production regions in France. The results show the prevalence of Verticillium wilt and, in the majority of cases and a1l production areas, the simultaneous presence of a number of other bioagressors, including fungi and nematodes. The isolates of Verticillium were identified at the species level (V. dahliae, V. isaacii) and characterized for their race (0 or 1) and their aggressiveness. Surprisingly, the aggressiveness ofmost isolates was relatively low. To our knowledge, this is the first report of V. isaacii in France, but its impact on production across France is unknown at this time.
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https://hal.science/hal-01595652/file/Daunay%2016th%20eucarpia%202016%20poster%20455-460_1.pdf BibTex
titre
A TILLING approach to generate broad-spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy
auteur
Jean-Luc Gallois, Camille Gauffier, Caroline Lebaron, Anna Bastet, Carole Caranta
article
International Advances in Plant Virology, Association of applied biologists, Sep 2016, London, United Kingdom
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https://hal.inrae.fr/hal-03195976/file/virology_2016_ann_pbf.pdf BibTex
titre
Linking genetics, physiology and modelling to gain insight in peach fruit quality
auteur
Bénédicte Quilot-Turion
article
11. National Meeting of the SFBV, Jul 2016, Angers, France
resume
absent
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https://hal.science/hal-01343638/file/Quilot-Turion%20SFBV%202016_1.pdf https://hal.science/hal-01343638/file/Quilot-Turion%20SFBV%202016_2.docx BibTex
titre
From domestication to modern breeding: what have learned from genetic resources in tomato?
auteur
Christopher Sauvage, Mathilde M. Causse
article
11. National Meeting of the SFBV, Jul 2016, Angers, France
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https://hal.inrae.fr/hal-02792606/file/Sauvage%20SFBV%202016_1.pdf https://hal.inrae.fr/hal-02792606/file/Sauvage%20SFBV%202016_2.docx BibTex
titre
The type III effector RipAX2 confers avirulence of [i]Ralstonia solanacearum[/i] to the eggplant AG91-25, carrying the resistance gene Ers1
auteur
Jérémy Guinard, Keke Wang, Fabien Lonjon, Lakshmi Sujeeun, Marco E. Mechan Llontop, Anne-Claire Noel, Patrick Barberis, Stéphane Genin, Marie-Christine Brand-Daunay, Jacques Dintinger, Stéphane Poussier, Boris Vinatzer, Nemo Peeters, Emmanuel Wicker
article
6. International Bacterial Wilt Symposium (IBWS), Jul 2016, Toulouse, France
resume
absent
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https://hal.science/hal-01343635/file/Daunay%206th%20IBWS%202016%20Poster_1.pdf BibTex
titre
From gene to phenotype: genetic control and modeling of sugar metabolism during peach fruit development
auteur
Elsa Desnoues, Valentina Baldazzi, Michel M. Génard, Patrick Lambert, Carole C. Confolent, Laure Heurtevin, Bénédicte Quilot-Turion
article
8. International Rosaceae Genomics Conference, Jun 2016, Angers, France
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https://hal.inrae.fr/hal-02798866/file/Quilot-Turion%208.%20Inter.%20Rosa.%20Geno.%20Conf.%202016_1.pdf https://hal.inrae.fr/hal-02798866/file/Quilot-Turion%208.%20Inter.%20Rosa.%20Geno.%20Conf.%202016_2.docx BibTex
titre
Joint estimation of effective population size and selection coefficient without neutrel markers: method validation and application to experimental evolution of viruses
auteur
Elsa Rousseau, Benoît Moury, Ludovic Mailleret, Rachid R. Senoussi, Alain Palloix, Vincent Simon, Frédéric Grognard, Sophie Valière, Frédéric Fabre
article
13. International plant virus epidemiology symposium, Jun 2016, Avignon, France. 165 p
resume
Experimental evolution studies deserve considerable attention to the estimation of basic evolutionary forces such as selection and genetic drift. With the advent of high-throughput sequencing techniques, these studies gained a renewed attention. However the joint estimation of selection and genetic drift still remain challenging when no neutral markers are available, a common situation with many microbes, such as viruses, due to their small tightly packed genomes.
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https://hal.inrae.fr/hal-02739698/file/Palloix%2013.int.%20plant%20virus%20epid.%20sympo.%202016%2016_1.pdf BibTex
titre
Impact of selection, genetic drift and viral accumulation on the evolution of a plant RNA virus
auteur
Lucie Tamisier, Gregory Girardot, Ghislaine Nemouchi, Marion Szadkowski, Alain Palloix, Benoît Moury
article
13. International plant virus epidemiology symposium, Jun 2016, Avignon, France. 165 p
resume
Genetic control is an efficient way to fight against crop disease, but this method is limited by the pathogen ability to evolve and break the resistance down. In the pepper-Potato virus Y (PVY) pathosystem, studies have demonstrated that the plant genetic background can increase the durability of the major resistance gene (Palloix et al., 2009), especially by constraining pathogen evolution (Quenouille et al., 2013). We conducted an experimental evolution to (i) measure the pathogen ability to adapt to plants combining major resistance gene and partially resistant genetic background and (ii) identify the role of the evolutionary forces induced by the genetic background on virus evolution.
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https://hal.inrae.fr/hal-02739150/file/2016_Tamisier_Avignon_1 BibTex
titre
A priori durable resistance in tomato to Phytophthora infestans using its pathogenicity effectors
auteur
Emmanuel Szadkowski, Gaëtan Maillot, Alexandre Bachellez, Anne Massire, Bernard Caromel, Véronique Lefebvre
article
Journées Jeunes Chercheurs du DBAP, Institut National de Recherche Agronomique (INRA). UMR Institut Jean-Pierre BOURGIN (1318)., Apr 2016, Lyon, France
resume
A priori durable resistance in tomato to Phytophthora infestans using its pathogenicity effectors. Journées Jeunes Chercheurs du DBAP
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Prunus - Réalités et perspectives de l'écologisation en arboriculture fruitière
auteur
Claire Lamine, Servane Penvern, Lucile Garcon, Sibylle Bui, Jean Pluvinage, Sylvaine Simon, Guy Clauzel, Jean Marc Audergon
article
PRUNUS, Mar 2016, Saint Marcel lès Valence, France. 12 p
resume
Le projet « PRUNUS- Réalités et perspectives de l’écologisation en arboriculture fruitière » a été financé par le programme Pesticides* du ministère de l’environnement sur la période 2013-2015 avec pour objectif d’identifier les effets de verrouillage et les leviers de transition vers une écologisation des pratiques agricoles (réduction des pesticides) dans les vergers de pêchers et d’abricotiers de la basse Vallée du Rhône.
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https://hal.science/hal-01313366/file/2016_Lamine_Prunus.pdf BibTex
titre
Mise en place d'un réseau national d'observations harmonisées de la phénologie chez les espèces fruitières.
auteur
Marc M. Bonhomme, Pascale Guillermin, Jean-Michel J.-M. Legave, José Quero-Garcia, Teresa Barreneche, Gilles Orain, Jean-Marc Ebel, V. Mathieu, Jean Marc Audergon
article
Séminaire ACCAF "Recherche et Adaptation au Changement Climatique", Mar 2016, Magny le Hongre, France. 1 p
resume
Dans le cadre du projet ACCAF PerPheClim, la phénologie a été étudiée en tant qu’indicateur de l’impact des changements climatiques. Pour appréhender son évolution, la mise en place d’observatoires implantés en des sites contrastés et suivis sur le long terme est apparue particulièrement utile. De tels observatoires, existaient pour la vigne et les espèces forestières mais pas pour les espèces fruitières (Domergues et al.,2004). Il a donc été décidé de mettre en place un dispositif national en réseau adapté à révéler les interactions génotype x environnement chez les espèces fruitières (Legave, 2009) et d’accompagner le dispositif par la mise en oeuvre de protocoles de suivis harmonisés.
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Des étiquettes gustatives pour les abricots? Une expérimentation économique
auteur
Sandrine Costa-Migeon Costa, Zouhair Bouhsina, Danièle Scandella, Maxime Sebbane, Jean Marc Audergon, Barbara Gouble
article
Colloque restitution ILLIAD, Jan 2016, Montpellier, France
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titre
Caractérisation variétale de la texture des abricots, réponse à la cuisson et analyse des mécanismes
auteur
Barbara Gouble, Albert Ribas-Agusti, Patrice Reling, Sylvie Bureau, Carine Le Bourvellec, Marielle Boge, Jamal Ayour, Catherine M.G.C. Renard, Guy Clauzel, Alain Blanc, Jean Marc Audergon
article
Journées de Restitution du projet ANR-ILLIAD, Jan 2016, Montpellier, France
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La mécanisation de la récolte d’abricots : faisabilité technique, conduite des vergers et comportements des variétés
auteur
Barbara Gouble, Jean Michel Broquaire, Christophe Chamet, Eric Hostanou, Patrice Reling, Sylvie Bureau, Marielle Boge, Adrienne Samper, Jamal Ayour, Catherine M.G.C. Renard, Jean Marc Audergon
article
Journées de Restitution du projet ANR-ILLIAD, Jan 2016, Montpellier, France
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Identification des principaux facteurs biochimiques impliqués dans la réponse différentielle des variétés d’abricot au traitement thermique
auteur
Albert Ribas-Agusti, Barbara Gouble, Sylvie Bureau, Jean Francois Maingonnat, Jean Marc Audergon, Mohamed El Maataoui, Catherine M.G.C. Renard
article
Journées de Restitution du projet ANR-ILLIAD, Jan 2016, Montpellier, France
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titre
Deciphering the genetic control of tomato fruit composition in flavonoids in the resequencing Era
auteur
Mathilde M. Causse, Laura Pascual, Christopher Sauvage, Elise Albert, Jean-Paul Bouchet, Dominique D. Brunel, Marie-Christine Le Paslier
article
24. International Plant and Animal Genome Conference, Jan 2016, San Diego, United States
resume
Identifying the genes controlling the variation of quantitative traits is a key goal for breeders. Genetic variations underlying quantitative traits (QTL) have been mapped by linkage mapping for years and positional cloning identified several QTLs. However linkage mapping is limited to the analysis of traits differing between two lines and the impact of genetic background on QTL effect has been underlined. Multi-allelic Advanced Generation Inter-Cross (MAGIC) populations allow a wide range of variability to be analyzed. We have constructed a MAGIC population by crossing 8 tomato lines, representing a wide range of genetic diversity within the species. The large range of phenotypic variability represented by these lines was then characterized at different scales, metabolomic, proteomic and transcriptomic. The whole genomes of the 8 founder lines were resequenced identifying more than 4 millions SNPs when mapped onto the reference genome. A set of 1536 SNPs markers was then selected to genotype the MAGIC population and used to construct a linkage map. A large increase in recombination frequencies compared to bi-parental populations was shown. QTLs for fruit composition traits were mapped and related to the variations detected at the genome sequence and expression levels in the parental lines. QTL for flavonoid compounds were mapped and candidate genes identified will be presented
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titre
A draft assembly of the almond genome
auteur
Tyler S. Alioto, Fernando Cruz, Jessica Gómez-Garrido, Leonor Frias, Paolo Ribeca, Marta Gut, Konstantinos Alexiou, Werner Howad, Jordi Morata, Josep Casacuberta, Maria J. Rubio-Cabetas, Beatriz Galán, José Luis García, Amit Dhingra, Henri Duval, Angel Fernandez I Marti, Michelle Wirthensohn, Ivo G. Gut, Pere Arús
article
24. International Plant and Animal Genome Conference, Jan 2016, San Diego, United States
resume
Almond is one of the oldest cultivated nut crops with its origin in central and western Asia. The selection of the sweet type [i](Prunus dulcis[/i]) distinguishes the domesticated almond from its bitter wild relatives. It is economically important, especially in California with the highest worldwide production, followed by Australia and Spain. The almond belongs to the same subgenus as the peach, for which there already exists a reference genome. However, to fully understand the genetic underpinnings marking the key phenotypic differences between almond and peach, we have sequenced the genome of the ‘Texas’ almond, one of the traditional cultivars producing a sweet nut. Whole-genome shotgun sequencing of Illumina paired-end libraries gave an initial low-contiguity assembly of 512 Mbp, nearly double the estimated genome size. Counting of k-mers indicates a 275 Mbp genome with substantial heterozygosity as well as repetitive sequence. In order to tackle both problems, we constructed a fosmid library and sequenced 68 pools of ~500 clones per pool. We then assembled the pools, merged them and finished the assembly by scaffolding with paired end and mate pair libraries, which resulted in a 240 Mbp assembly with a scaffold N50 of 500 kbp, a contig N50 of 33.5 kbp and CEGMA completeness of 99%. Two thirds of the assembly was anchored to the peach-almond genetic map, and using re-sequencing data of peach-almond hybrids and their parents we inferred the two haplotypes of the sequenced almond tree. We performed additional validation of the assembly using Oxford Nanopore MinION sequencing.
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Conference papers
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https://hal.science/hal-01344926/file/Duval%20Plant%20and%20Animal%20Genome%202016%201-5_1.pdf https://hal.science/hal-01344926/file/Duval%20Plant%20and%20Animal%20Genome%20XXIV%20Conference%202016_2.htm BibTex

Lectures

titre
Prenez-en de la graine ! Les légumes secs du jardin à l’assiette
auteur
Marie Josephe Amiot-Carlin, Michel Pitrat, Pascal Coquin
article
2016
resume
Consommées depuis plusieurs millénaires, les légumineuses seraient parmi les premières plantes à avoir été cultivées, avec les céréales. Présentes dans de nombreuses recettes traditionnelles à travers le monde, elles ont aujourd’hui toute leur place dans notre alimentation grâce à leurs vertus nutritionnelles et écologiques.
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Lectures
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https://hal.inrae.fr/hal-02798501/file/2016_Expo%20itin%C3%A9rante%20STC_LEGIMINEUSES_1.pdf BibTex

Other publications

titre
Méthodes et dispositifs innovants pour l'évaluation du matériel végétal fruitier, MEDIEVAL
auteur
Sylvie Colleu, Marine Guadagnini-Palau, Jean Marc Audergon, Daniel Plénet, Jean-Luc Regnard, Benoit Jeannequin, Sandrine Codarin, Christian Hilaire, Vincent Mathieu, Julien Ruesch, Yann Bintein
article
2016, pp.33
resume
Ce rapport, rédigé par un groupe de travail du Groupement d'Intérêt Scientifique, GIS Fruits a eu deux objectifs. D'une part dresser un état des lieux et réaliser une analyse critique des dispositifs actuels d’évaluation du matériel végétal fruitier. D'autre part, identifier les nouveaux outils, méthodes et dispositifs qui pourraient être mobilisés pour améliorer l'évaluation du matériel végétal fruitier et faciliter la connaissance et le transfert des informations, ainsi que repérer les pistes de recherches complémentaires pour développer de nouveaux outils et méthodes.
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Other publications
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https://hal.science/hal-01603980/file/Rapport%20de%20Synth%C3%A8se%20M%C3%A9di%C3%A9val_VF-VF_1.pdf BibTex
titre
Des espèces légumières venues du monde entier
auteur
Michel Pitrat
article
2016, pp.1-4
resume
Quelques légumes font encore localement l’objet de cueillette des formes sauvages : la mâche, le pissenlit et d’autres « salades » récoltés dans les prés, les jeunes pousses d’asperge sauvage ([i]Asparagus acutifolius[/i]) ou de tamier dans les haies et les sous-bois, sans oublier tous les champignons sylvestres. Cependant la plupart des légumes que nous consommons sont des formes domestiquées et cultivées. Quelle est l’origine des légumes consommés régulièrement en France et en Europe ? Depuis les travaux de de Candolle, Vavilov, Harlan, nous avons beaucoup d’éléments de réponse à cette question, même s’il subsiste des incertitudes, en particulier à cause de la grande diversité des espèces légumières.
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Other publications
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https://hal.science/hal-01312108/file/2016_Pitrat%20_Jardins%20de%20France%20640_1.pdf BibTex

Poster communications

titre
Improving modern breeding in tomato: the crucial role of genetic resources
auteur
Christopher Sauvage, Mathilde Causse
article
13. Japan Solanaceae Consortium (JSOL2016), Nov 2016, Tokyo, Japan. 2016
resume
The tomato is one of the most produced vegetable in the world and this species is also a model in science to the study of fruit development. Over the past 60 years, many sampling campaigns in Central and South America (source area) helped preserving more than 80,000 accessions of this species, including most of its crop wild relatives. In parallel, the contribution of molecular biology through the development of molecular markers was used to map many QTLs for traits of agronomic interest such as fruit quality and resistance to biotic and abiotic stresses. Since 2012, date of publication of the tomato genome, the combination of the rich genetic resources and high-throughput genomic was used to extend the work to a genome-wide scale to better understand its dynamic, to identify large amount of polymorphic sites but also revisiting scientific questions, such as the evolutionary history (i.e. domestication) of this species and the study of the genetic architecture of quantitative traits through new approaches such as genome-wide association study (GWAS) or genomic selection genomics. The genetic resources of this species are essential for fundamental scientific research but also for the genetic improvement of this species.
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Poster communications
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https://hal.science/hal-01604959/file/Sauvage%20JSOL%202016_1.pdf https://hal.science/hal-01604959/file/Sauvage%20JSOL%202016_2.docx BibTex
titre
Genetic and genomic control of tomato adaptation to water stress
auteur
Elise Albert, Laura Pascual-Banuls, Christopher Sauvage, Jean-Paul Bouchet, Frederique Bitton, Isidore Diouf, Matthieu Beukers, Yolande Carretero, Mathilde Causse
article
Argentina Plant Physiology meeting, Nov 2016, Corrientes, Argentina. , 2016
resume
Genetic and genomic control of tomato adaptation to water stress. Argentina Plant Physiology meeting
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titre
Effect on water deficit on tomato fruit quality - Genetic variability of the fruit response
auteur
Nadia Bertin, Beatrice Brunel, Julien Bonnefoi, Ivana Petrović, Mathilde Causse
article
3. International Symposium on Horticulture in Europe, SHE2016, Oct 2016, Chania, Greece. , 2016
resume
Water deficit is known to improve fleshy fruit quality at the expense of yield. However in tornato, recent studies showed that such effects are highly dependent on genotypes. Thus, breeding tomato plants adapted to grow under limited water supply requires a better understanding of the effects of WD on fruit quality and their genetic variability. ln the present work, four experiments have been performed under greenhouse conditions on the 8 parents of the Multi-Parent Advanced Generation Inter-Cross population which offers the largest allelic variability observed in tomato. Moderate and intensive WDs have been applied during the whole period of fruit development The experiments were performed either in spring or in autumn to analyze the interactions between WD and season. Moreover, interaction between moderate WD and plant fruit load was measured in spring. Fruit quality was assessed through fresh and dry masses, and fruit composition (soluble sugars, organic acids, vitamin C, carotenoids). Results show that fruit fresh mass is reduced p,roportionally to WD intensity, whereas the % dry mass is increased. Big fruit size genotypes are much more sensitive to WD than small fruit size genotypes. The improvement of fruit taste, based on the increase of the sugar:acid ratio, resulted more from concentration effects than from metabolic trigger effects. Trade-offs between fruit size decrease and quality improvement allowed discriminating the 8 accessions for their genetic interest under water limited conditions.
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titre
Genetic dissection of tomato fruit quality in the genome era: new tools for in depth QTL characterization
auteur
Elise Albert, Laura Pascual-Banuls, Christopher Sauvage, Janejira Duangjit, Jean-Paul Bouchet, Frederique Bitton, Isidore Diouf, Yolande Carretero, Mathilde Causse
article
3. International Symposium on Horticulture in Europe, SHE2016, Oct 2016, Chania, Greece. 2016
resume
Tomato fruit quality is characterized by a large number of components influenced by the genotypes and the environment. To better understand t~e genetic control of these components, quantitative trait loci (QTL) have been mapped for years. Today the genome sequence availability changes the paradigm of genetic approaches. QTL have been first identified using linkage mapping populations and positional cloning identified a few QTLs. However Iinkage mapping is Iimited to the analysis of traits differing between two parentallines. Genome-wide association (GWA) has then been proposed to assess a large range ofvariability. ln tomato, we have shown that GWAstudy is possible, using the admixed nature of cherry tomato genomes that reduces the impact of population structure. Nevertheless, GWAsuccess is limited in sorne regions due to the low decay of linkage disequilibrium, which varies along the genome. Rare alleles are also difficult to detect with GWA studies. Multi-parent advanced generation intercross (MAGIC) populations offer an alternative to traditionallinkage and GWAs by increasing the precision of QTLmapping but with equilibrated allelic frequencies. We have developed a MAGICpopulation by crossing eight tomato lines whose genomes were resequenced. We showed the potential of the MAGIC population when coupled with whole genome sequèncing to detect candidate single nuc\eotide polymorphisms (SNP) underlying the QTLs. QTLs for fruit quality traits were mapped and related to the variations detected at the genome sequence and expression levels. The advantages and limitations of the three types of population, in the context of the available genome sequence and resequencing facilities, will be discussed.
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Poster communications
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https://hal.science/hal-01604736/file/Albert_Sauvage_Causse%20SHE%202016%2045_1.pdf BibTex
titre
PICLÉG': a "research, development and stakeholders" all-together initiative for integrated production of field vegetables in France
auteur
Mathilde Causse, Vincent Faloya, Benoit Jeannequin
article
3. International Symposium on Horticulture in Europe, SHE2016, Oct 2016, Chania, Greece. 2016
resume
PICLÉG': a "research, development and stakeholders" all-together initiative for integrated production of field vegetables in France. 3. International Symposium on Horticulture in Europe, SHE2016
typdoc
Poster communications
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https://hal.science/hal-01602854/file/Causse%20SHE%202016%20195%20poster_1.pdf BibTex
titre
Transcriptomic regulation in pepper during the interaction with Phytophthora capsici
auteur
Gaëtan Maillot, Emmanuel Szadkowski, Anne Massire, Jean-Paul Bouchet, Veronique Brunaud, Marie-Laure Martin-Magniette, Sandrine Balzergue, Guillem Rigaill, Kurt Lamour, Véronique Lefebvre
article
16. Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary. 2016
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Poster communications
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https://hal.inrae.fr/hal-02799453/file/Maillot%20Eucarpia%20Hungary%202016%20poster_1.pdf BibTex
titre
GWAS of tomato response to water deficit: focus on major fruit quality traits
auteur
Elise Albert, Vincent Segura, Justine Gricourt, Romain Novaretti, Yolande Carretero, Esther Pelpoir, Julien Bonnefoi, Laurent Derivot, Mathilde Causse
article
13. Solanaceae Conference, Sep 2016, Davis, United States. 2016
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Poster communications
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https://hal.inrae.fr/hal-02799533/file/Albert_Causse%202016%20Poster_1.pdf BibTex
titre
Transcriptomic regulation in pepper during the interaction with Phytophthora capsici.
auteur
Gaëtan Maillot, Emmanuel Szadkowski, Anne Massire, Jean-Paul Bouchet, Veronique Brunaud, Martin-Magniette Marie-Laure, Sandrine Balzergue, Guillem Rigaill, Kurt Lamour, Véronique Lefebvre
article
16th Eucarpia Capsicum and Eggplant Meeting, Sep 2016, Kecskemét, Hungary
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titre
Influence of iron availability on tomato fruit quality
auteur
Ester Izquierdo Alegre, Pierre Fourcroy, Jossia Boucherez, Geneviève Conejero, Nicolas Tissot, Christophe Rothan, Cécile Bres, Jean-Philippe Mauxion, Mathilde Causse, Patricia Siso Terreza, M Alvarez-Ferandez, Javier Abadia, Jean-Francois Briat, Frédéric Gaymard, Christian Dubos
article
11e Colloque National de la Société Française de Biologie Végétale SFBV, Jul 2016, Angers, France. 2016
resume
Influence of iron availability on tomato fruit quality. 11e Colloque National de la Société Française de Biologie Végétale SFBV
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titre
The type III effector RipAX2 confers avirulence of #Ralstonia solanacearum# to the eggplant AG91-25, carrying the resistance gene Ers1
auteur
Jérémy Guinard, Keke Wang, Fabien Lonjon, Lakshmi Sujeeun, Marco E. Mechan Llontop, Anne-Claire Cazalé, Patrick Barberis, Stéphane Genin, Marie-Christine Daunay, Jacques Dintinger, Stéphane Poussier, Boris A. Vinatzer, Nemo Peeters, Emmanuel Wicker
article
6th IBWS, Jul 2016, Toulouse, France. 2016
resume
no abstract
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titre
De la ferme à la fourchette : TRADITOM Un nouveau projet de recherche de l'UE sur la valorisation des variétés traditionnelles de tomate
auteur
Brigitte Navez, Valentine Cottet, Raphael Tisiot, Mathilde Causse, Lilian Fontanet
article
Journées Portes Ouvertes du CTIFL, Jun 2016, Balandran, France. 2016
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Poster communications
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https://hal.inrae.fr/hal-02794688/file/Causse%202016%20Poster_1.pptx https://hal.inrae.fr/hal-02794688/file/Causse%202016%20Poster_2.pptx BibTex
titre
Exploring plant tolerance to viruses as a sustainable means of disease control
auteur
Judith Hirsch, Gregory Girardot, Alain Palloix, Benoît Moury
article
13. International plant virus epidemiology symposium, Jun 2016, Avignon, France. , 165 p., 2016, “Building bridges between disciplines for sustainable management of plant virus diseases”. IPVE 2016. Programme and abstracts
resume
The use of resistant plant cultivars is an efficient, cost-effective and environmentally-friendly method of disease control, particularly against viral pathogens. However, resistance is subject to breakdown, through the often rapid adaptation of viral populations to newly deployed resistant genotypes. As it exerts a weaker selection pressure on parasite populations, plant tolerance to parasites - the mechanisms that reduce the negative impact of infection on host fitness or yield, despite relatively elevated parasite concentrations - appears as an interesting alternative to resistance sensu stricto - mechanisms that reduce pathogen accumulation (1). However, the durability of tolerance has seldom been experimentally tested, and counter-examples exist (2). Moreover, littie is known about the genetic determinants controlling tolerance to pathogens. We have chosen the interaction between pepper and Cucumber mosaic virus (CMV) as a model to study plant tolerance to viral pathogens and evaluate the durability of tolerance.
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Poster communications
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https://hal.inrae.fr/hal-02744161/file/Palloix%202016%20poster%2034_1.pdf BibTex
titre
Resistance sources against a new an old world begomoviruses associated with epidemic diseases in curcubit crops
auteur
Gustavo Romay, Cecile Desbiez, Catherine Wipf-Scheibel, Pauline Millot, Michel Pitrat, Hervé Lecoq
article
13. International plant virus epidemiology symposium, Jun 2016, Avignon, France. , 165 p., 2016, “Building bridges between disciplines for sustainable management of plant virus diseases”. IPVE 2016. Programme and abstracts
resume
The cucurbits include several important vegetables to human consumption which are threatened for epidemic diseases such as those caused by begomoviruses. The Old World begomovirus Tomato leet curl New Delhi virus (ToLCNDV) was first described in India and is currently spreading in some Mediterranean countries. Meanwhile, the New World begomovirus Melon chlorotic mosaic virus (MeCMV) has been only described in Venezuela. This work aims to evaluate melon lines in order to look for resistance sources against these viruses.
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Poster communications
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https://hal.inrae.fr/hal-02740930/file/Pitrat%202016%20poster%2074_1.pdf BibTex
titre
The resistance to virus triggered by aphid inoculation in Vat melon is not systemic
auteur
Pascale Mistral, Nathalie Boissot
article
13. International Plant Virus Epidemiology Symposium, Jun 2016, Avignon, France. 177 p., 2016
resume
Host plant resistance is an essential mean of controlling virus epidemies in crops. Resistance to viruses in plant belongs to three major families, either recessive or dominant resistance genes, as weil as the antiviral defense system based on RNA silencing. Most NBS-LRR antiviral resistances are triggered by the recognition of the NBS-LRR protein and a virus protein, playing the raie of avirulence factor. The Vat resistance gene in melon is unique among the dominant resistance to virus genes. It is a CC-NBS-LRR gene, it is triggered by the recognition between an aphid avirulence factor, delivered in plant cells by Aphis gossypii puncturing, and the CC-NBS-LRR pratein produced by Vat plants. This resistance is efficient against unrelated viruses transmitted on the non persistent mode (Boualem et al., 2016). We investigated if the resistance to virus triggered by A. gossypii in Vat plants is systemic.
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Poster communications
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https://hal.inrae.fr/hal-02739588/file/Mistral_Boissot%202016%20poster%2054_1.pdf BibTex
titre
Impact of iron availability on tomato fruit quality
auteur
Ester Izquierdo Alegre, Pierre Fourcroy, Jossia Boucherez, Geneviève Conejero, Nicolas Tissot, Christophe Rothan, Cécile Bres, Jean-Philippe Mauxion, Mathilde Causse, Patricia Siso Terreza, M Alvarez-Ferandez, Javier Abadia, Jean-Francois Briat, Frédéric Gaymard, Christian Dubos
article
XVIII International Symposium on Iron Nutrition and Interactions in Plants, May 2016, Madrid, Spain. 2016
resume
Impact of iron availability on tomato fruit quality. XVIII International Symposium on Iron Nutrition and Interactions in Plants
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titre
Microstructural changes in cooked apricots related to the loss of texture
auteur
Albert Ribas-Agusti, Barbara Gouble, Jean Marc Audergon, Mohamed El Maataoui, Catherine M.G.C. Renard
article
2nd Euro-Mediterranean Symposium on Fruit and Vegetable Processing, Apr 2016, Avignon, France. 2016
resume
Introduction. The quality of the apricot products is largely defined by their texture, which is significantly lost upon processing. The pectin properties of apricots may play an important role in this loss of texture, even more than the initial firmness, but this is not yet well understood. This study aims to identify a varietal differentiation in the texture-loss properties and to link them with microstructural changes, with a final goal of understanding the mechanisms that influence the texture loss in apricot. Materials and Methods. Fruits were selected from four varieties at similar ripening stages, which were determined by their firmness using a compression test]. The apricot halves were cooked at 85°C in sugar syrup and their texture was measured by the maximum force in the penetrometry test. Microstructure changes were revealed by ruthenium red staining (pectin) and LM19 immunolabelling (unmethylated and low-methylated pectin). Results and Discusson. Variety largely influenced the loss of texture. Orangered was the variety with higher resistance to the texture loss (67% of the initial firmness), followed by Hargrand (74%), Iranien (75%) and Goldrich (86%). The tissues did not show severe damaging after cooking, however, some microstructural changes could be observed. The cells lost turgor as demonstrated by the higher sinuosity of the cell walls. Some intercellular spaces swelled, creating round-shaped microstructures filled with unmethylated pectin, as showed by LM19. Their presence across the varieties showed to be in line with their resistance to the loss of texture. Varietal differences in the biochemical properties must have prompted the ease of pectin to be leached from the tissue or to be retained within the intercellular spaces. Conclusions. We identified a link between the creation of intercellular microstructures and the loss of texture. This study opens the possibility of understanding the creation of these microstructures and the biochemical properties that are implied, which is in the interest of the food industry for the elaboration of higher quality apricot products.
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Poster communications
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https://hal.inrae.fr/hal-02738899/file/FVP16_Ribas_microstrutural%20changes_poster_1.pdf BibTex
titre
Impact of cooking and storage on apricots fruit microconstituents.
auteur
Carine Le Bourvellec, Sylvie Bureau, Patrice Reling, Romain Bott, Albert Ribas, Jean Marc Audergon, Catherine M.G.C. Renard, Barbara Gouble
article
2nd Euro-Mediterranean Symposium on Fruit and Vegetable Processing, Apr 2016, Avignon, France. 2016
resume
Apricots are a rich source of phenolics and carotenoids that may contribute to reduced risks of chronic diseases. However, because of the seasonal availability, apricots are commonly consumed after processing and storage. The different thermal processing methods have been shown to reduce polyphenolic and carotenoid levels in fruit. However information is lacking on how different methods and storage influence the phenolic and carotenoid composition and their content in processed apricots. Four apricot cultivars were processed in two different ways: home cooking (85°C) and industrial processing (95°C). Cans from industrial processing were analyzed immediately and after storage of 2 months at ambient temperature. Phenolics and carotenoids were quantified by HPLC with diode array detection. Industrial processing caused a significantly higher loss of total phenolics compared to domestic cooking. Upon cooking, procyanidins were retained in apricot tissues while flavan-3-ol monomers and hydroxycinnamic acids were partially leached into the syrup. Due to leaching of flavan-3-ol monomer procyanidin’s DPn significantly increased from fruit to canned products. Anthocyanins were not significantly altered by heat treatment. Total carotenoids exhibited no significant change after heat processing. However among carotenoids, a cis-β-carotene isomer was significantly increased after industrial processing, but with lower amounts after domestic cooking. No significant losses were observed for total phenolic compounds after two months in cans at ambient temperature, this was mainly due to procyanidins which were stable over time. However, flavan-3-ol monomers, hydroxycinnamic acids and anthocyanins were significantly lower in the fruit after storage. Total carotenoids compounds significantly decreased over the storage period. Based on HPLC data, carotenoids were more stable than phenolics after heat treatment. Carotenoids and phenolics in fruit decreased with storage time. More interestingly, this study indicates that apricots are a rich source of procyanidins, having profiles similar to those found in apples. Processing changed not only the content of procyanidins in apricot products but also the relative ratio of the different molecular weights.
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titre
Apricot texture: variability as a function of cultivar, influence of maturity and impact of cooking
auteur
Jamal Ayour, Barbara Gouble, Patrice Reling, Albert Ribas, Jean Marc Audergon, Mohamed Benichou, Catherine M.G.C. Renard
article
2nd Euro-Mediterranean Symposium on Fruit and Vegetable Processing, Apr 2016, Avignon, France. 2016
resume
Apricot (Prunus armeniaca L.) is a fleshy fruit of real economic and nutritional interest. The rapid loss of fruit firmness is a decisive factor for quality and to decide apricot use (fresh or processed) and commercialization ways (short or storage). To better understand the texture heterogeneity in apricot, we studied the impact of the harvest stage and the effect of a heat treatment on a large range of cultivars.
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Poster communications
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https://hal.inrae.fr/hal-02742833/file/FVP16_Ayour_apricot%20texture_poster_1.pdf BibTex

2015

Conference papers

titre
Recherche de résistance aux nématodes à galles chez la laitue
auteur
Brigitte Maisonneuve
article
Rencontres du GIS PIClég, Groupement d'Intérêt Scientifique pour la Production Intégrée en Cultures légumières (Gis Piclég). FRA., Nov 2015, Paris, France
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titre
A la recherche de méthodes alternatives aux tests biologiques classiques pour la détermination de la date de sortie d'endodormance, la quête du haut débit par le Groupe de Travail Dormance de Perpheclim et d'ODS.
auteur
Marc Bonhomme, Jean-Paul Charpentier, Vincent Segura, Luc Pâques, Jean Marc Audergon, José Quero-Garcia, Elisabeth Dirlewanger, Rémi Beauvieux, Bénédicte Wenden, Jean-Michel Legave, Isabelle Farrera, Hendrik Davi, Frederic Jean, Isabelle Chuine
article
Colloque francophone PHENOLOGIE, Nov 2015, Clermont-Ferrand, France. 135 p
resume
Pour améliorer les modélisations de la phénologie, nous avons besoin de dates de débourrement et de floraison mais aussi de dates de levée de dormance, sur un grand nombre d’espèces. On est à la recherche de méthodes alternatives aux tests classiques, peu onéreuses et permettant de s’approcher du haut débit. Une approche en spectrométrie proche infrarouge montre une bonne corrélation entre NIRS et paramètre classique de dormance (DMD) sur poudre de bourgeons végétatifs et floraux. Une autre approche concernant la visualisation de callose au niveau de la zone méristématique, considérée comme un marqueur d’état dormant, a été entreprise.
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Conference papers
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https://hal.science/hal-01580642/file/2015_Bonhomme_Orale_Ph%C3%A9no_%7BF8DC2941-1275-420D-B6C7-3EC6F8507BC5%7D.pdf BibTex
titre
Metabolic genome-wide-association unravels trait architecture and natural variation in tomato primary and secondary metabolisms
auteur
Guillaume Bauchet, Stéphane Grenier, Nicolas Samson, Vincent Segura, Aniko Kende, Jules Beekwilder, Katarina Cankar, Arnaud G. Bovy, Jean-Luc Gallois, Julien Bonnet, Laurent Grivet, Elek Segura, Charles Segura, Mathilde Causse
article
12. Solanaceae Conference, Oct 2015, Bordeaux, France
resume
absent
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https://hal.science/hal-01269376/file/Bauchet_Causse%202015%2012th%20Solanaceae%20Conference%2041_1.pdf BibTex
titre
Assessment of factors affecting genomic selection in populations of tomato
auteur
Coralie Picard, Janejira Duangjit, Mathilde M. Causse, Christopher Sauvage
article
12. Solanaceae Conference, Oct 2015, Bordeaux, France
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https://hal.inrae.fr/hal-02795275/file/Causse_Sauvage%2012th%20Solanaceae%20Conference%2030_1.pdf BibTex
titre
Modelling the population history using population genomics : the tomato domestication as a case of study
auteur
Christopher Sauvage, Charlotte Aichholz, Gautier Sarah, Manuel Ruiz, Mathilde Causse, Jacques David, Sylvain Glemin
article
12. Solanaceae Conference, Université de Bordeaux (UB). FRA., Oct 2015, Bordeaux, France
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https://hal.science/hal-01269378/file/Sauvage_Causse%202015%2012th%20Solanaceae%20Conference%2097_1.pdf BibTex
titre
Genotype by watering regime interaction in cultivated tomato: from phenotypes to genes
auteur
Elise Albert, Yolande Carretero, Justine Gricourt, Esther Pelpoir, Romain Novaretti, Claire Duffes, Julien Bonnefoi, Nadia Bertin, Frederique Bitton, Stephanie Pateyron, Jean-Philippe Tamby, Mathilde M. Causse
article
12. Solanaceae Conference, Oct 2015, Bordeaux, France
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https://hal.inrae.fr/hal-02743885/file/Albert_Causse%202015%2012th%20Solanaceae%20Conference%2084_1.pdf BibTex
titre
TALEN and CRISPR strategies for targeted editing of the plant genome
auteur
Marianne Mazier, Fabien F. Nogué
article
CORESTA AP2015, Oct 2015, Izmir, Turkey
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https://hal.inrae.fr/hal-02801029/file/Mazier%202015%20Coresta%20AP_1.pdf https://hal.inrae.fr/hal-02801029/file/Mazier%202015%20Coresta%20AP_2.docx BibTex
titre
Toward a characterization of the pepper host resistance effect on the gene expression of the pathogenic Phytophthora capsici
auteur
Gaëtan Maillot, Anne Massire, Jean-Paul Bouchet, Véronique Brunaud, Marie-Laure Martin-Magniette, Sandrine Balzergue, Kurt Lamour, Véronique Lefebvre
article
12. Solanaceae Conference, Oct 2015, Bordeaux, France
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https://hal.inrae.fr/hal-02795452/file/Maillot_Lefebvre%202015%2012th%20Solanaceae%20Conference%2062_1.pdf BibTex
titre
Genomic variation in tomato, from wild to cultivated
auteur
José Blanca, Javier Montero-Pau, Christopher Sauvage, Guillaume Bauchet, Eudald Illa, Maria José Diez Niclos, David Francis, Mathilde M. Causse, Esther van Der Knaap, Joaquîn Cafiizares
article
12. Solanaceae Conference, Oct 2015, Bordeaux, France
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https://hal.inrae.fr/hal-02800701/file/Causse%202015%2012th%20Solanaceae%20conference%2027_1.pdf BibTex
titre
Vitamin C and cell wall metabolisms in tomato: GDP-D-mannose epimerase (GME) a key actor of these interrelated pathways
auteur
Louise Gilbert, Marie Dumont, Carine Ferrand, Celine Bournonville, Antoine Monier, Joana Jorly, Isabelle Atienza, Michel Hernould, Rebecca Stevens, Arnaud Lehner, Jean-Claude Mollet, Christophe Rothan, Patrice Lerouge, Pierre Baldet
article
The 12th Solanaceae Conference SOL 2015, Oct 2015, Bordeaux, France
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https://hal.inrae.fr/hal-02739180/file/Stevens%202015%2012th%20Solanaceae%20Conference%2050_1.pdf BibTex
titre
Pectinmethylesterase activity heterogeneity in apricot fruit
auteur
Albert Ribas-Agusti, Barbara Gouble, Jean Marc Audergon, Catherine M.G.C. Renard
article
EuroFoodChem XVIII, Oct 2015, Madrid, Spain
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Yield prediction based on QTLs for yield components using crop eco-physiological models
auteur
M. Wubs, Egbert Heuvelink, J. J. Magan, Alain Palloix, Fredericus van Eeuwijk
article
MODEL-IT, Symposium on modelling for Horticulture, Oct 2015, Wageningen, Netherlands
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https://hal.inrae.fr/hal-02796656/file/Palloix%202015%20Symp.%20model.%20Hort._1.pdf https://hal.inrae.fr/hal-02796656/file/Palloix%202015%20Symp.%20model.%20Hort._2.docx BibTex
titre
Identification de géniteurs de résistance à des nématodes à galles du genre Meloidogyne chez la laitue
auteur
Brigitte Maisonneuve
article
Journée nationale Salade, Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL). FRA., Oct 2015, Bellegarde, France
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RNA-Seq study of host resistance and isolate aggressiveness on gene expression in [i]Phytophthora capsici[/i]
auteur
Gaëtan Maillot, Anne Massire, Jean-Paul Bouchet, Veronique Brunaud, Marie-Laure Magniette, Sandrine Balzergue, Kurt Lamour, Véronique Lefebvre
article
8. Effectome Meeting, Sep 2015, Lauret, France
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absent
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https://hal.science/hal-01210021/file/Maillot%202015%20effectome%20abstract_1.pdf https://hal.science/hal-01210021/file/Maillot%202015%20effectome%20abstract_2.docx BibTex
titre
Effect of shrinkage on prediction accuracy of methionine and proline fruit contentsin a broad-based tomato population
auteur
Janejira Duangjit, Mathilde M. Causse, Christopher Sauvage
article
Genomics, Bioinformatics and System Biology Conference (GBSBC 2015), Sep 2015, Bangkok, Thailand
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https://hal.inrae.fr/hal-02739886/file/Causse_Sauvage%202015%20GBSBC%201-6_1.pdf BibTex
titre
Spatial pattern of Eurasian woolly mammoth extinctions during the Late Pleistocene based on statistical inference from fossil records
auteur
Frédérik Saltré, Joel J. Chadoeuf, B.W. Brook, Damien A. Fordham, Marta Rodriguez-Rey, Corey J.A. Bradshaw
article
9. IALE World Congress, International Association of Landscape Ecology (IALE). USA., Jul 2015, Portland Oregon, U.S. Virgin Islands
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https://hal.inrae.fr/hal-02793379/file/Chadoeuf%202015%20abstract_1.pdf https://hal.inrae.fr/hal-02793379/file/Chadoeuf%202015%20abstract_2.docx BibTex
titre
The PERPHECLIM ACCAF Project - perennial fruit crops and forest phenology evolution facing climatic changes
auteur
Inaki Garcia de Cortazar Atauri, Jean Marc Audergon, Patrick Bertuzzi, Christel Anger, Marc Bonhomme, Isabelle Chuine, Hendrik Davi, Sylvain Delzon, Eric Duchêne, Jean-Michel Legave, Helene Raynal, Christian Pichot, Cornelis van Leeuwen
article
XVI International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. pp.89 - 93, ⟨10.17660/ActaHortic.2018.1214.15⟩
resume
Phenology is a bio-indicator of climate evolution. Measurements of phenological stages on perennial species provide actually significant illustrations and assessments of the impact of climate change. Phenology is also one of the main key characteristics of the capacity of adaptation of perennial species, generating questions about its consequences on plant growth and development or on fruit quality. Predicting phenology evolution and adaptive capacities of perennial species needs to override three main methodological limitations: 1) existing observations and associated databases are scattered and sometimes incomplete, rendering difficult implementation of multi-site study of genotype-environment interaction analyses 2) there are not common protocols to observe phenological stages 3) access to generic phenological models platforms is still very limited. In this context, the PERPHECLIM project, which is funded by the Adapting Agriculture and Forestry to Climate Change Meta-Program ( ACCAF) from INRA ( French National Institute of Agronomic Research), aims to develop the necessary infrastructure at INRA level ( observatories, information system, modeling tools) to enable partners to study the phenology of various perennial species ( grapevine, fruit trees and forest trees). Currently, the PERPHECLIM project involves 28 research units in France, mainly from INRA institutes. Five activities have been developed: define protocols and observation forms to observe phenology for various species of interest for the project : organize observation training, develop generic modeling solutions to simulate phenology ( Phenological Modelling Platform software and modelling platform solutions), support the building of research projects at national and international levels, develop environment/genotype observation networks for fruit-tree species, and develop an information system to manage data and documentation concerning phenology. Finally, the PERPHECLIM project aims to build strong collaborations with public ( Observatoire des Saisons) and private ( technical institutes) sector partners in order to allow a more direct transfer of knowledge.
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titre
Infrared spectroscopy as a rapid tool to assess apricot fruit quality
auteur
Sylvie Bureau, Catherine M.G.C. Renard, Jean Marc Audergon
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China
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https://hal.inrae.fr/hal-02742207/file/Audergon%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2013_1.pdf BibTex
titre
Solanaceae pollen tubes and vegetative organs have structurally divergent cell wall xyloglucan
auteur
Flavien Dardelle, François Le Mauff, Corinne Loutelier-Bourhis, Muriel Bardor, Christophe Rihouey, Mathilde Causse, Patrice Lerouge, Azeddine Driouich, Jean-Claude Mollet, Arnaud Lehner
article
International Symposium on Plant Signaling and Behavior (PSB, 3, 2015), Jun 2015, Paris, France
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Aramis®" strategy: a durable manner to develop a sharka resistant apricot cultivar
auteur
Guillaume Roch, Jean Marc Audergon, Thierry Candresse, Véronique Decroocq, David Tricon, Sylvie Dallot, Magaly Lamberet, Guy Clauzel
article
16. International Symposium on Apricot Breeding and Culture, International Society for Horticultural Science (ISHS). INT., Jun 2015, Shenyang, China. 298 p
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https://hal.inrae.fr/hal-02744190/file/Roch_Audergon%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2026-27_1.pdf BibTex
titre
Genetic structure of a worldwide germplasm collection of Prunus armeniaca L. species
auteur
Hedia Bourguiba, Jean Marc Audergon, Boris Krška, Tatyana Zhebentyayeva, Arnaud A. Remay, Claudio d'Onofrio, Craig A. Ledbetter, Hiroyuki Iketani, Danilo Christen, Weisheng Liu, Guillaume Roch
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. ⟨10.17660/ActaHortic.2018.1214.35⟩
resume
Analyses of genetic diversity and phylogenetic relationships illuminate the origin and domestication of crop species and have important implications for plant breeding programs and the conservation of genetic resources. Apricot ( Prunus armeniaca L.) genetic resources in collections that are widely dispersed around the world were assessed using 25 simple sequence repeat ( SSR) markers. Analysis of allelic diversity among SSR data revealed at least a double pattern of diffusion from Central Asia to eastern and western countries with a clear east-west loss of genetic diversity related to the genetic bottleneck during apricot domestication. Structure and phylogenetic analysis indicated that accessions from Central Asia and China were genetically most diverse, suggesting that this large region constitutes the apricot center of origin.
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https://hal.inrae.fr/hal-02736957/file/Audergon_Bourguiba%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2020-21_1.pdf BibTex
titre
Mechanical harvest: a realistic challenge for the processed apricot market?
auteur
Barbara Gouble, Patrice Reling, Catherine M.G.C. Renard, J. M. Broquaire, C. Chamet, Jean Marc Audergon
article
16. International Symposium on Apricot Breeding and Culture, International Society for Horticultural Science (ISHS). INT., Jun 2015, Shenyang, China. pp.5, ⟨10.17660/ActaHortic.2018.1214.9⟩
resume
ILLIAD is a French national project in which food-chain sustainability is being evaluated on four products ( wheat, rice, peach and apricot). For apricot, two difficulties for French production have been identified: i) the high cost of manpower in this culture and ii) fruit processors' dependence on surplus fruits from fresh consumption. This leads to very high variability in supply, price and quality from year to year. In this context, one solution might be dedicated orchards for processing industries. At first, cultivars well adapted to processing and with a low demand for inputs were screened for their behavior in mechanical harvest. Mechanical harvest was carried out using tree shaking ( at 40 cm from the ground) and collection on a fabric screen. Results obtained over 2 years on more than 15 cultivars and in two production areas are presented. The low mechanical damage observed just after harvesting or after 7 days of storage for a few cultivars and the possibility of optimizing shaking parameters according to agronomic and physiologic criteria ( fruit falling, maturity heterogeneity, etc.) confirm the suitability of mechanical harvesting and the opportunity to create dedicated orchards.
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Selections issued from CEP Innovation, Centrex and INRA: the apricot breeding program in France
auteur
G. Fremondiere, Alain Blanc, Florent Gilles, Guy Clauzel, J. M. Broquaire, Guillaume Roch, Barbara Gouble, Sylvie Bureau, C. Pitiot, Jean Marc Audergon
article
16. International Symposium on Apricot Breeding and Culture, International Society for Horticultural Science (ISHS). INT., Jun 2015, Shenyang, China. pp.3, ⟨10.17660/ActaHortic.2018.1214.36⟩
resume
French apricot production is located in the south-eastern part of the country, with a focused zone in the Rhone valley and close to the Mediterranean Sea. The north of the Rhone valley is characterized by a continental climate, while the southern region ( Rhone valley and Mediterranean area) is under a warm, typically Mediterranean climate. Obviously, apricot fruit production depends on the development of a specific panel of cultivars in each area of cultivation because of economic and adaptation constraints. In order to enlarge the panel of cultivars for growers of each region, an integrated breeding program has been developed at a national level in France with the participation of INRA, Centrex and CEP Innovation. The main goals of the breeding program are: the elaboration of cultivars suitable for durable production ( including resistance to pests and diseases), production regularity ( including self-fertility and quality of the flowers), and fruit quality ( including taste, attractiveness, and suitability for transport). The evaluation phase is organized in the three main French areas of production ( Perpignan, Nimes and Valence) with three complementary evaluation stages: ( i) observation of hybrids on their own roots ( ii) evaluation of pre-selections after grafting in the three main areas, and ( iii) 'elite' agronomic and commercial assessments in small orchards. A tough diagnosis, that includes biological, serological and molecular analysis, is conducted in parallel for assessing resistance to Plum pox virus ( PPV). The main results issued from this program are presented here, with 10 cultivars, including PPV-resistant Aramis (R) cultivars.
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Functional analysis of Mlo genes in powdery mildew resistance in melon
auteur
Sonia Elbelt, Nathalie Giovinazzo, Verane Sarnette, Marc Bardin, Christelle Troadec, Abdelhafid A. Bendahmane, Catherine Dogimont
article
5. ISHS International Symposium, Curcubits 2015, Jun 2015, Murcia, Spain
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https://hal.inrae.fr/hal-02744309/file/Elbert_Dogimont%202015%205.%20ISHS%20Int.%20Sympo.%20Cucurbits%2043_1.pdf BibTex
titre
Assessing brown rot resistance in peach fruit
auteur
I. Pacheco, Leandro de Oliveira-Lino, A. Ciacciulli, M. Lama, S. Foschi, F. Faoro, Veronique Signoret, D. Bassi, Bénédicte Quilot-Turion
article
XIV Eucarpia Symposium on Fruit Breeding and Genetics, Jun 2015, Bologne, Italy
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https://hal.inrae.fr/hal-02740286/file/Quilot_%20Bologna_2015_1.pdf BibTex
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Discovery of peach QTLs by using pedigree-based analysis
auteur
José Ramon Hernandez, Diego Micheletti, Marco Bink, Eric van de Weg, Daniele Bassi, Nelson Nazzicari, Andrea Caprera, Maria Teresa Dettori, Sabrina Micali, Elisabeth Dirlewanger, Patrick Lambert, Thierry Pascal, Elisa Bianchi, Laura Rossini, Ignazio Verde, Bénédicte Quilot-Turion, Francois Laurens, Pere Arús, Maria Jose Aranzana
article
14. Eucapia Fruit breeding and genetics symposium, Jun 2015, Bologne, Italy. 2 p
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Genetic and genomic control of response to water deficit in cultivated tomato
auteur
Elise Albert, Yolande Carretero, Justine Gricourt, Esther Pelpoir, Romain Novaretti, Claire Duffes, Julien Bonnefoi, Mathilde M. Causse
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, International Society for Horticultural Science (ISHS). INT., Jun 2015, Avignon, France
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https://hal.inrae.fr/hal-02744107/file/Albert_Causse%202015%20ISHS%20Avignon_1.pdf BibTex
titre
Host factors for brown rot resistance in peach fruit
auteur
Leandro de Oliveira-Lino, Michel M. Génard, Bénédicte Quilot-Turion
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, International Society for Horticultural Science (ISHS). INT., Jun 2015, Avignon, France. 56 p
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https://hal.inrae.fr/hal-02738605/file/DeOliveiraLino_Quilot_Avignon_2015_1.pdf BibTex
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Screening tomato germplasm for resistance to late blight
auteur
Bernard Caromel, Coralie Hamers, Nasradin Touhami, Alexandre Renaudineau, Alexandre Bachellez, Anne Massire, Rene Damidaux, Véronique Lefebvre
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, Jun 2015, Avignon, France
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https://hal.inrae.fr/hal-02744090/file/Caromel_Bachellez%202015%20ISHS%20Avignon_1.pdf BibTex
titre
A pyramiding strategy for resistance to rootknot nematodes meloidogyne spp. in prunus rootstock material
auteur
Henri Duval
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, International Society for Horticultural Science (ISHS). INT., Jun 2015, Avignon, France
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https://hal.inrae.fr/hal-02738895/file/Duval%202015%20ISHS%20Avignon_1.pdf BibTex
titre
Physical host factors for brown rot resistance in peach fruit
auteur
Leandro de Oliveira-Lino, Michel Génard, Véronique Signoret, Bénédicte Quilot-Turion
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, International Society for Horticultural Science (ISHS). INT., Jun 2015, Avignon, France. ⟨10.17660/ActaHortic.2016.1137.15⟩
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Brown rot in peach fruits caused by the fungi Monilinia sp. is a common disease that can provoke as much as 30 to 40% of crop losses. Little is known about the fruit resistance factors and the infection process. The aim of this study was to investigate the physical factors of resistance of the fruit and their genetic control. This would provide a tool to rationalize genotype x cultural practice combinations in order to reduce brown rot incidence. Mechanical characteristics of fruit skin (conductance, stomatal density, microcracks) potentially linked to Monilinia (M.) resistance were investigated in 27 cultivars. Two cultivars and a segregating population were phenotyped for fruit cuticular conductance and sensitivity was assessed by two independent infection tests. In the microscopy analyses, high diversity of germination and penetration of fungi through stomata and microcracks were observed. Preliminary results show significant differences in the number of stomata among genotypes. However, no obvious relationship was found between stomata number, cuticular conductance and infection at the early stage of fruit development. At maturity, the population displayed high variability in the resistance to M. laxa, giving hope for breeding programs, despite the low stability of results among tests and years. The knowledge gained from these experiments will be integrated into an existing ecophysiological model in order to concurrently optimize fruit characteristics and cultural practices to reduce infection risks.
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Assessment of diseases susceptibility of peach cultivars in experimental plots and on-farm for organic and low-input systems. Baseline of french case studies
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Claude Eric Parveaud, Johanna Brenner, Sophie-Joy Ondet, Christelle Gomez, Gilles Libourel, François Warlop, Laurent Brun, Vincent Mercier, Guy Clauzel, Jean Marc Audergon
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, International Society for Horticultural Science (ISHS). INT., Jun 2015, Avignon, France
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https://hal.inrae.fr/hal-02738751/file/Audergon_Clauzel%202015%20ISHS%20Avignon_1.pdf BibTex
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Combinations of varietal and technical innovations for the sustainable and integrated management or root-knot nematodes: the gedumen project
auteur
Caroline Djian-Caporalino, Ariane Fazari, Nathalie Marteu, Mireille Navarrete, Arnaud Dufils, Marc Tchamitchian, Claudine Furnion, Amélie Lefevre, Laure Pares, Thierry Mateille, Johannes Tavoillot, Alain Palloix, Anne-Marie Sage-Palloix, Hélène Védie, Claire Goillon, Philippe Castagnone-Sereno
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, International Society for Horticultural Science (ISHS). INT., Jun 2015, Avignon, France
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https://hal.inrae.fr/hal-02739482/file/Palloix_Sage-Palloix%202015%20ISHS%20Avignon_1.pdf BibTex
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Joint estimation of effective population sizes and selection coefficients from time-sampled data: a case study on virus populations submitted to plant resistance
auteur
Elsa Rousseau, Frédéric Fabre, Jérôme Coville, Ludovic Mailleret, Alain Palloix, Rachid R. Senoussi, Frédéric Grognard, Benoît Moury
article
9. colloque de la Société Française de Phytopathologie, Societe Francaise de Phytopathologie (SFP). FRA., Jun 2015, Colmar, France
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https://hal.inrae.fr/hal-02738575/file/2015_Rousseau_SFP_1.pdf BibTex
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A kinetic model of sugar metabolism allows the simulation of sugar concentrations and its genetic variability during peach fruit growth
auteur
Elsa Desnoues, Michel M. Génard, Bénédicte Quilot-Turion, Valentina Baldazzi
article
10. International Symposium on Modelling in Fruit Research and Orchard Management, International Society for Horticultural Science (ISHS). INT., Jun 2015, Montpellier, France
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https://hal.inrae.fr/hal-02744196/file/Desnoues_Quilot%20Montpellier_2015_1.pdf BibTex
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Epidemic reconstruction for the inference of vector dispersal in fragmented landscapes
auteur
David Pleydell, Samuel S. Soubeyrand, Sylvie Dallot, Gerard Labonne, Joel J. Chadoeuf, Gael Thébaud
article
Risques et modèles en épidémiologie humaine, animale & végétale, Université des Antilles et de la Guyane (UAG). Pointe-à-Pitre, FRA., May 2015, Point-à-Pitre, France
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https://hal.inrae.fr/hal-02799006/file/sharka_short_1.pdf BibTex
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Estimation of genetic drift and selection from next generation sequencing time-sampled data
auteur
Elsa Rousseau, Frédéric Fabre, Jérôme Coville, Ludovic Mailleret, Alain Palloix, Rachid Senoussi, Frédéric Grognard, Benoît Moury
article
Modelling biological evolution 2015: linking mathematical theories with empirical realities, Apr 2015, Leicester, United Kingdom
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no abstract
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Effect of the lettuce genotype on the protective efficacy of plant defence stimulators against Bremia
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Brigitte B. Maisonneuve, Caroline Allen-Aubouard
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Eucarpia Leafy Vegetable 2015, Apr 2015, Murcia, Spain
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https://hal.inrae.fr/hal-02739598/file/Maisonneuve%20Eucarpia%202015_1.pdf https://hal.inrae.fr/hal-02739598/file/Maisonneuve%20Eucarpia%202015_2.docx BibTex
titre
PERPHECLIM ACCAF Project perennial fruit crops and forest phenology evolution facing climatic changes.
auteur
Inaki Garcia de Cortazar Atauri, Jean Marc Audergon, Patrick P. Bertuzzi, Christel Anger, Marc M. Bonhomme, Isabelle Chuine, Hendrik Davi, Sylvain S. Delzon, Eric Duchêne, Jean-Michel J.-M. Legave, Helene H. Raynal, Christian Pichot, Cornelis van Leeuwen, . Perpheclim Team
article
General Assembly of the European Geosciences Union (EGU), Apr 2015, Vienne, Austria. pp.EGU2015-9846
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https://hal.inrae.fr/hal-02739606/file/2015_GarciadeCortazar_EGU_Abstract_1.pdf BibTex
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Déterminants génétiques du contournement de la résistance des solanacées maraîchères au virus Y de la pomme de terre : vers la compréhension et la gestion de la durabilité des résistances
auteur
Benoît Moury, Frédéric Fabre, Mekki Ben Khalifa, Hatem Fakhfakh, Alain Palloix
article
26. Forum International des Sciences Biologiques et de Biotechnologie, Association Tunisienne des Sciences Biologiques (ATSB). TUN., Mar 2015, Monastir, Tunisie
resume
De nombreux gènes de résistance sont utilisés chez les solanacées maraîchères (piment et tomate) pour lutter contre le virus Y de la pomme de terre (PVY). Ces résistances ont montré des durabilités très contrastées : certaines résistances sont efficaces depuis plus de 50 ans sans qu’il y ait émergence de souches adaptées (virulentes) de PVY alors que pour d’autres résistances, des souches virulentes sont déjà présentes dans les cultures avant même l’utilisation de variétés porteuses de ces résistances par les agriculteurs. L’identification des mutations du PVY responsables du contournement de ces différentes résistances et de leur impact sur le pouvoir pathogène du virus ont permis de comprendre les raisons de ces disparités. Par ailleurs, une approche de modélisation mathématique des événements biologiques en jeu dans le contournement des résistances a permis de définir des stratégies optimales d’utilisation des variétés cultivées dans un paysage agricole. Ces résultats soulignent l’importance de la connaissance du déterminisme génétique du pouvoir pathogène chez les virus pour lutter efficacement et durablement contre les maladies qu’ils induisent.
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titre
Utilisation raisonnée des fongicides et apport des biofongicides pour lutter contre la fusariose des épis de blé
auteur
Hélène Batina, V. Atanasova-Penichon, A. Fourrey, Sandrine Gélisse, G. Grignon, Valérie Laval, C. Maumene, B. Meleard, Romain Valade, Anne-Sophie Walker, E. Gourdain
article
5ème conférence internationale sur les méthodes alternatives de protection des plantes, AFPP, Mar 2015, Lille, France
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titre
Le projet GEDUNEM : Innovations techniques et variétales pour une gestion durable & intégrée des nématodes à galles dans les systèmes maraîchers sous abris
auteur
Caroline Djian-Caporalino, Ariane Fazari, Nathalie Marteu, Mireille Navarrete, Arnaud Dufils, Marc Tchamitchian, Claudine Furnion, Amélie Lefevre, Laure Pares, Thierry Mateille, Johannes Tavoillot, Alain Palloix, Anne-Marie Sage-Palloix, Hélène Védie, Claire Goillon, I. Forest, Philippe Castagnone-Sereno
article
5. Conférence Internationale sur les Méthodes Alternatives de Protection des Plantes AFPP, Mar 2015, Lille, France
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Conference papers
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https://hal.inrae.fr/hal-02743394/file/Palloix_Sage-Palloix%202015%205.%20Conf.%20Lille_1.pdf https://hal.inrae.fr/hal-02743394/file/Palloix_Sage-Palloix%202015%205.%20Conf.%20Lille_2.doc BibTex
titre
GENIUS project. Genome ENgineering Improvement for Useful plants of a Sustainable agriculture
auteur
Fabien Nogué, Philippe Vergne, Anne-Marie Chèvre, Jean-Eric Chauvin, Oumaya Bouchabke-Coussa, Annabelle Dejardin, Elisabeth Chevreau, Laurence Hibrand-Saint Oyant, Marianne Mazier, Pierre Barret, Emmanuel Guiderdoni, Luc Mathis, Christophe Sallaud, Mireille Matt, Jean-Philippe Pierron, Eric Bonnel, Séverine Foucrier, Alain Toppan, Laure Trannoy, Peter Rogowsky
article
15. Rencontres de Virologie Végétale (RVV), Jan 2015, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.science/hal-01204208/file/Mazier%202015%20Aussois%20R%C3%A9sum%C3%A9%20du%20poster_2.pdf https://hal.science/hal-01204208/file/Mazier%202015%20Aussois%20poster_1.pdf https://hal.science/hal-01204208/file/Mazier%202015%20Aussois%20R%C3%A9sum%C3%A9%20du%20poster_3.docx BibTex
titre
Joint estimation of effective population sizes and selection coefficients from time-sampled data: a case study on virus populations exposed to plant resistance
auteur
Elsa Rousseau, Frédéric Fabre, Jérôme Coville, Ludovic Mailleret, Alain Palloix, Rachid R. Senoussi, Frédéric Grognard, Benoît Moury
article
15. Rencontres de Virologie Végétale (RVV), Jan 2015, Aussois, France
typdoc
Conference papers
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https://hal.inrae.fr/hal-02738942/file/2015_Rousseau_RVV_1.pdf BibTex
titre
The tomato-potyvirus system, a comparative system for natural and induced resistances associated with the initiation factors 4E
auteur
Camille Gauffier, Caroline Lebaron, André Moretti, C. Constant, F. Moquet, G. Bonnet, Carole Caranta, Jean-Luc J.-L. Gallois
article
15. Rencontres de Virologie Végétale, Jan 2015, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02794002/file/OK%28prod%29%20Gauffier%20T017%20Eucarpia%20tomato%202014_1.pdf BibTex
titre
Évaluer l’acceptabilité par les agriculteurs d’innovations variétales et agronomiques pour maîtriser les nématodes à galles en maraîchage sous abri (le projet GEDUNEM)
auteur
Mireille Navarrete, Claudine Furnion, Caroline Djian-Caporalino, Arnaud Dufils, Philippe Castagnone-Sereno, Alain Palloix, Marc Tchamitchian, Ariane Fazari, Nathalie Marteu, Anne-Marie Sage-Palloix, Amélie Lefevre, Laure Pares, Thierry Mateille, Johannes Tavoillot, Hélène Védie, Claire Goillon, Isabelle Forest
article
Les Rencontres du Végétal, AGROCAMPUS OUEST. FRA., Jan 2015, Angers, France. 10 p
resume
Root-knot nematodes (RKN, Meloidogyne spp.) is a major disease in sheltered vegetable crops, present on about 40% of the southern France farms (survey 2007 -2010). As most chemical nematicide products are now banned, it is necessary to find alternative solutions using agronomical techniques. New prototypes of cropping systems have been built previously in a participatory research involving scientists and technical advisors. They are based on RKN varietal resistances and on crop rotations alternating host, non-host species, soil solarisation and nematicide green manure. The Gedunem project aims at assessing the agronomical and nematological effects of these cropping systems (Caporalino et al 2014, same symposium), their mid-term sustainability, and their acceptability by farmers. The oral presentation illustrates this latter question. We compared the 3 cropping systems currently under trial and the actual cropping systems of 28 market-gardeners (Furnion 2014). Farmers were asked to explain if the 3 prototypes were acceptable or not depending on their own farm constraints and strategy. The major bottlenecks that were identified are the cost and time required for some practices (such as the use of resistant pepper as a trap-crop), and the availability of free land in summer on some crop rotations. Moreover the nature and intensity of the bottlenecks depend on the type of farm and the farmers’ motivation to innovate. This study enables to highlight how to adapt the present prototypes in order they may be implemented more easily on commercial farms.
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titre
Conception et évaluation d’innovations variétales et agronomiques pour maîtriser les nématodes à galles en maraîchage sous abri (le projet GEDUNEM)
auteur
Caroline Djian-Caporalino, Philippe Castagnone-Sereno, Ariane Fazari, Nathalie Marteu, Mireille Navarrete, Arnaud Dufils, Marc Tchamitchian, Claudine Furnion, Alain Palloix, Anne-Marie Sage-Palloix, Amélie Lefevre, Laure Pares, Thierry Mateille, Johannes Tavoillot, Hélène Védie, Claire Goillon, Forest Isbell
article
8. Rencontres du végétal, Jan 2015, Angers, France
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Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02801729/file/Palloix_Sage-Palloix%202015%20Renc.%20du%20V%C3%A9g%C3%A9tal_1.pdf BibTex
titre
GnpIS-Asso : A Generic Database for Managing and Exploiting Plant Genetic Association Studies Results Using High Throughput Genotyping and Phenotyping Data
auteur
Delphine Steinbach, Guillaume Merceron, Célia Michotey, Raphaël-Gauthier Flores, Nacer Mohellibi, Cyril Pommier, Christopher Sauvage, Stephane Nicolas, Pierre Dubreuil, Jacques Le Gouis
article
23. Plant and Animal Genome, Jan 2015, San Diego, United States
resume
We will present a new functionality developed in GnpIS information system to manage association studies data (GWAS). A query form is available on http://urgi.versailles.inra.fr/gnpis portal and directly at this URL https://urgi.versailles.inra.fr/association/association/viewer.do#form. The tool allows plant scientists or breeders to get associations values between traits and markers obtained in several association studies. Several filters are available to refine the query, such as the species, trait (s), or marker (s) or panel of germplasms. The tool gives also the possibility in a second round of analysis, to add a new set of filters, i) on statistical values, or ii) on treatment done in the study, or iii) on the location and iv) year of the phenotyping trial or according to the v) statistical model chosen to do the analysis. It allows to view graphically the results with dedicated plots (QQPlot, Manhattan Plot), generated dynamically and to extract data in files to continue the analysis with external tools. After selecting the best markersassociated to trait of interest, the tool allows also to automatically jump on the genome to find where this marker is located on chromosomes and to identify in a very simpler way which genes or other markers or features of interest are near it. This tool is already now used for dealing GWAS studies (association, genotyping and phenotyping data) for 2 species: tomato and maize, data that are already referenced in two 2 publications and will also manage wheat data in 2015.
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titre
Spatial pattern of Eurasian woolly mammoth extinctions during the Late Pleistocene based on statistical inference from fossil records
auteur
Frédérik Saltré, B.W. Brook, Joel J. Chadoeuf, Damien A. Fordham, Marta Rodriguez-Rey, Corey J.A. Bradshaw
article
International Biogeography Society (IBS 2015), Jan 2015, Bayreuth, Germany
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02792581/file/Chadoeuf%202015%20poster_1.pdf BibTex
titre
Estimation du niveau de dormance des bourgeons avec la NIRS, résultats préliminaires
auteur
Marc M. Bonhomme, Jean-Paul J.-P. Charpentier, Vincent Segura, Luc Pâques, Jean Marc Audergon, José Quero-Garcia, Jean-Michel J.-M. Legave, Isabelle Farrera, Hendrik Davi, Frédéric Jean
article
2. rencontres scientifiques du réseau NIRS INRA, 2015, Orléans, France. 2 p
resume
Pour améliorer les modélisations de la phénologie, nous avons besoin de dates de débourrement et de floraison mais aussi de dates de levée de dormance, sur un grand nombre d’espèces. On est à la recherche de méthodes alternatives aux tests classiques, peu onéreuses et permettant de s’approcher du haut débit. La NIRS était potentiellement un bon candidat. Les essais préliminaires montrent une bonne corrélation entre NIRS et paramètre classique de dormance (DMD) sur poudre de bourgeons végétatifs et floraux. La corrélation est moins bonne sur poudre de tige mais pourrait cependant constituer une alternative dans le cas de screening haut débit.
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02800715/file/2015_Bonhomme_NIRS_1.pdf BibTex

Master thesis

titre
Mise au point du ciblage génique chez la tomate
auteur
Célia Casset
article
Sciences du Vivant [q-bio]. 2015
typdoc
Master thesis
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02798194/file/Celia%20Caset%202015%20rapport_1.pdf BibTex
titre
Evaluation d'une stratégie de sélection génomique dans 3 dispositifs expérimentaux chez la tomate
auteur
Coralie Picard
article
Agricultural sciences. 2015
resume
La sélection pour améliorer les caractères d’intérêts agronomiques est réalisée à l’aide de la SAM ou de la GWA qui capturent difficilement les loci à effets mineurs, et grâce à des étapes de phénotypage souvent longues et couteuses. La sélection génomique (SG) est une méthode de prédiction de la performance des caractères via des modèles statistiques pour sélectionner des plantes élites dans un schéma de sélection, en estimant l’effet de tous les marqueurs qui sous-tendent l’architecture génétique des caractères étudiés. Nous avons évalué l’utilisation de la SG chez la tomate en estimant la précision de la prédiction pour 25 caractères liés à la qualité du fruit pour trois types de populations différentes (GWA, MAGIC et RIL). Une démarche de validation croisée a été menée en estimant le poids de paramètres (taille des populations d’entrainement (PE) et de validation (PV), interactions GxE, modèles statistiques prédictifs, densité de marquage, héritabilité des caractères, type de population) sur la précision de la prédiction. Nos résultats démontrent que l’approche SG semble puissante pour prédire les valeurs phénotypiques (avec des précisions allant de 0.32 à 0.93 lorsque la PE est optimisée, par exemple). Nous observons que (1) plus la PE est grande et plus elle contient des individus proches génétiquement et ayant été phénotypés dans des environnements similaires par rapport à la PV, plus la précision de la prédiction est élevée (2) la précision de la prédiction est plus élevée avec l’optimisation de la PE avec une grande diversité génétique (+ 9% en moyenne) (3) plus l’héritabilité du caractère est élevée, meilleure est la prédiction (4) les différents modèles statistiques conduisent à des prédictions très similaires (5) la densité de marqueurs optimale dépend du DL de la population. Pour conclure, bien que la SG soit une pratique qui n’en est qu’à ses débuts en ce qui concerne le monde du végétal l’application de la SG chez la tomate semble très prometteuse et pourrait être utilisée dans les programmes de sélection
typdoc
Master thesis
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02800021/file/2015_Picard_Coralie_Selection_genomique_tomate-6_1.pdf BibTex

Other publications

titre
Maladies et ravageurs émergents: stabilité pour la tomate
auteur
Michel Pitrat, Yannie Trottin-Caudal
article
2015, pp.21-22
resume
Les cultures de tomate peuvent être attaquées par de nombreux agents pathogènes et ravageurs, aussi bien aériens que telluriques. Suivant le type de culture (en plein champ, sous abris froids ou chauffés), l’importance relative de ces maladies et ravageurs est très différente.
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Other publications
Accès au texte intégral et bibtex
https://hal.science/hal-01312107/file/2015_Pitrat_Jardins%20de%20France%20633_1.pdf BibTex

Poster communications

titre
Stratégie de résistance durable aux nématodes à galles chez les porte-greffe de Prunus par pyramidage - Marquage à haute résolution d’un gène du pêcher et localisation fine d’un gène de l’amandier
auteur
Henri Duval, Cyril van Ghelder, Martin Masse, G. Roch, Hugo Hussie, Daniel Esmenjaud
article
Restitution des projets CTPS, Dec 2015, Paris, France. 2015
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02797924/file/Duval%202015%20Poster%20CTPS%20Pyredune_1.pdf BibTex
titre
Texture deterioration in cooked apricot: characterization of the loss of firmness and visualization of the unmethylated pectin in two varieties with very different patterns
auteur
Albert Ribas, Barbara Gouble, Jean Francois Maingonnat, Jean Marc Audergon, Mohamed El Maataoui, Catherine M.G.C. Renard
article
Fruit Structure, Nov 2015, Angers, France. 2015
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02796049/file/2015_Poster%20fruit%20structure%20Albert%20Ribas_1.pdf BibTex
titre
Mise en place d'un réseau national d'observations harmonisées de la phénologie chez les espèces fruitières
auteur
Marc M. Bonhomme, Pascale Guillermin, Jean-Michel J.-M. Legave, José Quero-Garcia, Teresa Barreneche, Gilles Orain, Jean-Marc Ebel, V. Mathieu, Jean Marc Audergon
article
Colloque francophone PHENOLOGIE, Nov 2015, Clermont-Ferrand, France. INRA UMR PIAF, 135 p., 2015, Colloque francophone Phénologie 2015
resume
Dans le cadre du projet ACCAF PerPheClim, la phénologie a été étudiée en tant qu’indicateur de l’impact des changements climatiques. Pour appréhender son évolution, la mise en place d’observatoires implantés en des sites contrastés et suivis sur le long terme est apparue particulièrement utile. De tels observatoires, existaient pour la vigne et les espèces forestières mais pas pour les espèces fruitières (Domergues et al.,2004). Il a donc été décidé de mettre en place un dispositif national en réseau adapté à révéler les interactions génotype x environnement chez les espèces fruitières (Legave, 2009) et d’accompagner le dispositif par la mise en oeuvre de protocoles de suivis harmonisés.
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02743106/file/2015_Bonhomme_Poster1_Ph%C3%A9no_1.pdf BibTex
titre
Back to the wild: identification and genetic mapping of resistance factors to Melampsora larici-populina in Populus nigra
auteur
Véronique Jorge, Arnaud Dowkiw, Redouane El Malki, Elise Albert, Marie Pégard, Vincent Segura, Vanina Guérin, Celine Ridel, Patrick Poursat, Jose-Luis Almeida Falcon, Patricia Faivre-Rampant, Catherine Bastien
article
5. International Workshop on the Genetics of Tree-Parasite Interactions, Aug 2015, Orléans, France. , 2015, 5th International Workshop on the Genetics of Tree-Parasite Interactions. Book of abstracts
resume
absent
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.science/hal-01269239/file/2015_Jorge_GTPI_comorale_1.pdf BibTex
titre
Are patterns of Populus nigra geographical differentiation for resistance to Melampsora larici-populina dependent of genetic variation of the pathogen populations?
auteur
Catherine Bastien, Arnaud Dowkiw, Patricia Faivre-Rampant, Elise Albert, Marc Villar, Patrick Poursat, Jose-Luis Almeida Falcon, Celine Ridel, Vanina Guérin, Brigitte Viguier, Marijke Steenackers, Véronique Jorge
article
5. International Workshop on the Genetics of Tree-Parasite Interactions, Aug 2015, Orléans, France. , 2015, 5th International Workshop on the Genetics of Tree-Parasite Interactions. Book of abstracts
resume
absent
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.science/hal-01269242/file/2015_Bastien_GTPI_comorale_1.pdf BibTex
titre
PERPHECLIM ACCAF Project - Perennial fruit crops and forest phenology evolution facing climatic changes
auteur
Iñaki García de Cortázar-Atauri, Jean Marc Audergon, Patrick Bertuzzi, Christel Anger, Marc Bonhomme, Isabelle Chuine, Hendrik Davi, Sylvain Delzon, Eric Duchêne, Jean-Michel Legave, Christian Pichot, Hélène Raynal, Cornelis van Leeuwen
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. 298 p., 2015
resume
Phenology is a bio-indicator of climate evolutions. Measurements of phenological stages on perennial species provide actually significant illustrations and assessments of the impact of climate change. Phenology is also one of the main key characteristics of the capacity of adaptation of perennial species, generating questions about their consequences on plant growth and development or on fruit quality. Predicting phenology evolution and adaptative capacities of perennial species needs to override three main methodological limitations: 1) existing observations and associated databases are scattered and sometimes incomplete, rendering difficult the implementation of multi-site study of genotype-environment interaction analyses; 2) there are no common protocols to observe phenological stages; 3) access to generic phenological models platforms is still very limited. In this context, the PERPHECLIM project, which is funded by the Adapting Agriculture and Forestry to Climate Change Meta-Program (ACCAF) from INRA, has the objective to develop the necessary infrastructure at INRA level (observatories, information system, modeling tools) to enable partners to study the phenology of various perennial species (grapevine, fruit trees and forest trees). Currently the PERPHECLIM project involves 27 research units in France. The main activities currently developed are: defining protocols and observation forms to observe phenology for various species of interest for the project; organizing observation training; developing generic modeling solutions to simulate phenology; supporting the building of research projects at national and international level; developing environment/genotype observation networks for fruit trees species; developing an information system managing data and documentation concerning phenology. Finally, PERPHECLIM aims to build strong collaborations with public (Observatoire des Saisons) and private sector partners (technical institutes) in order to allow a more direct transfer of knowledge.
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02743994/file/Audergon%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2041-42_%7B7ABB10B0-A482-4B0F-A381-27F8090AEF89%7D_1.pdf BibTex
titre
Selections issued from CEP INNOVATION-CENTREX and INRA apricot breeding program in France
auteur
G. Femondière, C. Pitiot, Alain Blanc, Frederic Gilles, Jean Marc Audergon, Guy Clauzel, J.M. Broquaire, Barbara Gouble, Sylvie Bureau
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. 2015
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02800714/file/Audergon%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2071_1.pdf BibTex
titre
Mechanical harvest: a realistic challenge for processed apricot market?
auteur
Barbara Gouble, Patrice Reling, Catherine M.G.C. Renard, Jean-Marc Broquaire, Christophe Chamet, Jean Marc Audergon
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. p. 38, 2015
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02794857/file/Audergon%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2038_1.pdf BibTex
titre
ILLIAD project: Sustainable, local or localised, innovative food chains – application to apricot production
auteur
Sylvie Bureau, Barbara Gouble, Jean Marc Audergon, Sandrine Costa-Migeon Costa, Selma Tozanli
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. 2018, ⟨10.17660/ActaHortic.2018.1214.7⟩
resume
ILLIAD (a French national project, 2012 - 2015) proposes a method to analyze the system sustainability of food chains. The sustainability of food systems has been defined in terms of their effects on environment, economics and society, until now. ILLIAD aims to propose a method that allow to take into account the ability of these food systems to perdure or develop, in the long run, and aims to access their ability to increase their positive effects on environment, economics and society.
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02736932/file/Bureau_Illiad%20project_China%202015_poster_1.pdf BibTex
titre
ILLIAD project: sustainable, local or localised, innovative food chains
auteur
Sandrine Costa-Migeon Costa, Sylvie Bureau, Selma Tozanli, Jean Marc Audergon
article
16. International Symposium on Apricot Breeding and Culture, Jun 2015, Shenyang, China. p. 44, 2015
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02740015/file/Audergon%202015%2016.%20Int.%20Symp.%20Apricot%20Chine%2044_1.pdf BibTex
titre
Exploring and exploiting phenotypic and genetic diversity in peach: identification of major genes and QTLs by GWAS
auteur
Maria Jose Aranzana Civit, Jose Ramon Hernandez Mora, D. Micheletti, S. Micali, N. Nazzicari, I. Pacheco, S. Foschi, Teresa Barreneche, Bénédicte Quilot-Turion, L. Wang, R. Ma, Xiaojian Li, I. Iglesias, J. Carbó, M. Troggio, E. Banchi, V. Aramini, M.T. Dettori, A. Caprera, C. da Silva Linge, Thierry Pascal, Patrick Lambert, Z. Gao, I. Verde, D. Bassi, L. Rossini, Francois Laurens, P. Arus
article
14. EUCARPIA Symposium on Fruit Breeding and Genetics, Jun 2015, Bologne, Italy. Acta Horticulturae, 2017, ⟨10.17660/ActaHortic.2017.1172.79⟩
resume
Genetic variability is a key requirement for breeding. Although new peach cultivars are released yearly to the market, the genetic pool of cultivated peaches is very limited. To evaluate the variability available in commercial but also in old local peach accessions we selected a panel of 1,580 accessions maintained and evaluated in four European and one Chinese germplasm collections. Phenotypic data collected over years following common protocols have been integrated in a database, generating a useful tool for breeders and researchers. These accessions were genotyped with the peach 9K SNP array v1. T. Genotypic data distributed the accessions in three main subpopulations (Occidental obtained in breeding programs, Occidental old local varieties and Chinese cultivars). Linkage disequilibrium (LD) was in agreement with previous studies reporting long extension. Phenotypic and genotypic data have been combined in a GWAS study allowing the design of markers for marker assisted selection (MAS). Preliminary analyses on quantitative traits are promising, while further analysis will be required to integrate all data in a single genome-wide association analysis.
typdoc
Poster communications
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BibTex
titre
Genome ENgineering Improvement for Useful plants of a Sustainable agriculture
auteur
Fabien F. Nogué, Philippe Vergne, Anne-Marie Chèvre, Jean-Eric Chauvin, Oumaya Bouchabke, Annabelle Dejardin, Elisabeth E. Chevreau, Laurence Hibrand-Saint Oyant, Marianne Mazier, Pierre P. Barret, Emmanuel Guiderdoni, Luc Mathis, Christophe Sallaud, Mireille Matt, Jean-Philippe Pierron, Eric Bonnel, Séverine Foucrier, Alain Toppan, Laure Trannoy, Peter Rogowsky
article
IUFRO Tree Biotechnology Conference 2015; Treebiotech2015, Jun 2015, Florence, France. 2015, Tree Biotechnology 2015 Conference "Forests: the importance to the planet and society"
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02743151/file/2015_Nogue_TB_S4P1_1.pdf https://hal.inrae.fr/hal-02743151/file/2015_IUFRO_Tree%20Biotechnology_program_2.pdf BibTex
titre
GIS fruits - medieval working group an initiative dedicated to the prospective evaluation of fruit species
auteur
Benoit B. Jeannequin, Marine Guadagnini-Palau, Daniel Plénet, Yann Bintein, Jean Marc Audergon
article
Innovation in Integrated & Organic Horticulture, INNOHORT 2015, Jun 2015, Avignon, France. 2015
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02792488/file/Audergon%202015%20ISHS%20Avignon_1.pdf BibTex
titre
Joint estimation of the strength of genetic drift and selection from next generation sequencing time-sampled data : a case study on the adaptation of virus populations to host plant resistance
auteur
Elsa Rousseau, Frédéric Fabre, Jérôme Coville, Ludovic Mailleret, Alain Palloix, Rachid R. Senoussi, Benoît Moury, Frédéric Grognard
article
SMBE Satellite meeting SMBEBA 2015 "Investigating biological adaptation with NGS: data and models", May 2015, St Martin de Londres, France. , 2015
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https://hal.inrae.fr/hal-02797375/file/Abstract_1 BibTex
titre
Genetic diversity of almond rootstocks. The INRA Prunus rootstock breeding program
auteur
Henri Duval
article
16. Grempa Meeting on Almonds and Pistachios, May 2015, Meknès, Morocco. 2015
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Poster communications
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https://hal.inrae.fr/hal-02801445/file/Duval%202015%20XVI%20grempa%20meeting_1.pdf BibTex
titre
Estimation of genetic drift and selection from next generation sequencing time-sampled data
auteur
Elsa Rousseau, Frédéric Fabre, Jérôme Coville, Ludovic Mailleret, Alain Palloix, Rachid R. Senoussi, Frédéric Grognard, Benoît Moury
article
Modelling biological evolution 2015: linking mathematical theories with empirical realities, Apr 2015, Leicester, United Kingdom. , 2015
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https://hal.inrae.fr/hal-02738798/file/PosterFabre_1.pdf BibTex
titre
PERPHECLIM ACCAF Project - Perennial fruit crops and forest phenology evolution facing climatic changes
auteur
Iñaki García de Cortázar-Atauri, Jean Marc Audergon, Patrick Bertuzzi, Christel Anger, Marc Bonhomme, Isabelle Chuine, Hendrik Davi, Sylvain Delzon, Eric Duchêne, Jean-Michel Legave, Christian Pichot, Hélène Raynal, Cornelis van Leeuwen
article
3. Climate Smart Agriculture, Mar 2015, Montpellier, France. , 298 p., 2015, CSA15 : Parallel Session L2 Climate Smart Strategies
resume
Phenology is a bio-indicator of climate evolutions. Measurements of phenological stages on perennial species provide actually significant illustrations and assessments of the impact of climate change. Phenology is also one of the main key characteristics of the capacity of adaptation of perennial species, generating questions about their consequences on plant growth and development or on fruit quality. Predicting phenology evolution and adaptative capacities of perennial species needs to override three main methodological limitations: 1) existing observations and associated databases are scattered and sometimes incomplete, rendering difficult the implementation of multi-site study of genotype-environment interaction analyses; 2) there are no common protocols to observe phenological stages; 3) access to generic phenological models platforms is still very limited. In this context, the PERPHECLIM project, which is funded by the Adapting Agriculture and Forestry to Climate Change Meta-Program (ACCAF) from INRA, has the objective to develop the necessary infrastructure at INRA level (observatories, information system, modeling tools) to enable partners to study the phenology of various perennial species (grapevine, fruit trees and forest trees). Currently the PERPHECLIM project involves 27 research units in France. The main activities currently developed are: defining protocols and observation forms to observe phenology for various species of interest for the project; organizing observation training; developing generic modeling solutions to simulate phenology; supporting the building of research projects at national and international level; developing environment/genotype observation networks for fruit trees species; developing an information system managing data and documentation concerning phenology. Finally, PERPHECLIM aims to build strong collaborations with public (Observatoire des Saisons) and private sector partners (technical institutes) in order to allow a more direct transfer of knowledge.
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titre
Genetic determinants of response to water deficit in cultivated tomato fruits – QTL x E analysis
auteur
Elise Albert, Claire Duffes, Julien Bonnefoi, Justine Gricourt, Yolande Carretero, Esther Pelpoir, Mathilde M. Causse
article
The Genome Analysis Centre, Jan 2015, Norwich, United Kingdom. 2015
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https://hal.inrae.fr/hal-02799805/file/Albert%202015%20TGAC%20poster_1.pdf BibTex
titre
A new elF4E allele characterized by RNAseq in tomato is associated with resistance to potyviruses
auteur
Caroline Lebaron, Christopher Sauvage, Camille Gauffier, Benoît Moury, Jean-Luc J.-L. Gallois
article
15. Rencontres de Virologie Végétale, Jan 2015, Aussois, France. 2015
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titre
Role of the genetic background on elF4E-based resistance to a PVY strain in tomato
auteur
Camille Gauffier, Caroline Lebaron, André Moretti, C. Constant, F. Moquet, G. Bonnet, Carole Caranta, Jean-Luc J.-L. Gallois
article
15. Rencontres de Virologie Végétale, Jan 2015, Aussois, France. 1 p., 2014
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Reports

titre
Conception d’idéotypes culturaux adaptés à la contrainte hydrique et améliorés sur des critères de qualité gustative et nutritionnelle – Cas d’étude: la tomate
auteur
Mathilde M. Causse, Nadia Bertin
article
[Contrat] Vilmorin; Gautier semences. 2015
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https://hal.inrae.fr/hal-02793458/file/OK%20CR%20ctps%20mars2016-final_1.pdf https://hal.inrae.fr/hal-02793458/file/OK%20CR%20ctps%20mars2016-final_2.docx BibTex
titre
High quality tomate varieties for the fresh and processing markets: Fl GARANCE and Fl TERRADOU
auteur
Rene Damidaux, Anne Lespine, . Asirpa (analyse Socio‐économique Des Impacts de La Recherche Publique Agronomique), . Département Bap
article
[Rapport Technique] INRA. 2015
resume
Research in genetics and disease carried out by INRA in close collaboration with the sector since the 1970s has led to the creation of two emblematic varieties: The F1 GARANCE tomato was originally intended to explore other ways of producing fruit of excellent organoleptic quality while at the same time responding to demands from the sector (fewer treatments, good tolerance of handling). Two innovative points maximise the gustatory potential of F1 GARANCE: its harvest at full ripeness and its storage at ambient temperature. Registration of the F1 TERRADOU tomato, with a very high dry matter content and multiple resistance to diseases, opened the way towards the development of a quality‐based payment grid for producers. The impact on energy use in this sector is highly significant. The qualities of these two tomatoes offer strong indicators for future opportunities to be explored by breeders and producers.
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https://hal.inrae.fr/hal-02794423/file/2015_Damidaux_Projet%20ASIRPA_1.pdf https://hal.inrae.fr/hal-02794423/file/2015_Damidaux_Projet%20ASIRPA_ENG_2.pdf BibTex
titre
Amélioration et homogénéisation des procédures touchant aux bases de données, à l'assurance qualité et la conservation de graines dans le CRB Légumes
auteur
Marie-Christine Daunay, Jérémy Salinier
article
[Contrat] 2015
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titre
Projet ANR VirAphid. Comment utiliser les résistances des plantes pour gérer durablement l'évolution des virus et des pucerons
auteur
Nathalie Boissot, Benoît Moury, Flavie Vanlerberghe-Masutti
article
[Contrat] Institut National de la Recherche Agronomique (INRA). 2015
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Preprints, Working Papers, ...

titre
Joint estimation of the strength of genetic drift and selection from next generation sequencing time-sampled data : a case study on the adaptation of virus populations to host plant resistance
auteur
Elsa Rousseau, Frédéric Fabre, Jérôme Coville, Ludovic Mailleret, Alain Palloix, Rachid Senoussi, Benoît Moury, Frédéric Grognard
article
2015
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no abstract
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2014

Conference papers

titre
Comment utiliser les résistances des plantes pour gérer durablement l'évolution des virus et des pucerons ?
auteur
Nathalie Boissot
article
Programme ANR SYSTERRA: "Agricultures et écosystèmes : du développement durable aux changements globaux - Des programmes thématiques pour impulser des recherches nouvelles ?, Agence Nationale de la Recherche (ANR). FRA., Dec 2014, Paris, France
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titre
Conception d'associations en maraîchage de plein champ : exemple de production de melons associés à des bandes fleuries pour lutter contre les pucerons et les virus
auteur
Alexandra Schoeny, Nathalie Boissot, Jérôme Lambion, Catherine Wipf-Scheibel, Pascale Mistral, Patrick Gognalons, Karine Nozeran, Hervé Lecoq
article
CIAG 2014 : Carrefours de l'Innovation Agronomique "Associations Végétales", Nov 2014, Angers, France
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https://hal.inrae.fr/hal-02744024/file/2014%2011%2020%20CIAg%20Schoeny_1.pdf BibTex
titre
Les acides dicaféoylquiniques comme molécules aphicides de biocontrôle
auteur
Marie-Hélène Sauge, Yvan Rahbé, Gérard Febvay, Jean-Luc Poessel
article
7. Rencontres du réseau Biologie Adaptative des Pucerons et Organismes Associés (BAPOA), Réseau Biologie Adaptative des Pucerons et des Organismes Associés (BAPOA). Le Rheu, FRA., Nov 2014, Avignon, France
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titre
Des bandes fleuries pour lutter contre les pucerons et les virus en culture de melons : retour d’expérience(s)
auteur
Alexandra Schoeny, Nathalie Boissot, Jérôme Lambion, Catherine Wipf-Scheibel, Pascale Mistral, Patrick Gognalons, Karine Nozeran, Hervé Lecoq
article
Journées du réseau Biologie et Adaptation des Pucerons et Organismes Associés (BAPOA), Nov 2014, Avignon, France
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titre
Profil de résistance aux pucerons conféré par le gène Vat: apport des plantes transgéniques
auteur
Veronique Chovelon, Nathalie Boissot, Catherine Dogimont
article
7. journées du réseau Biologie et Adaptation des Pucerons et Organismes Associés, Réseau Biologie Adaptative des Pucerons et des Organismes Associés (BAPOA). FRA., Nov 2014, Avignon, France
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titre
Approaches of the genetic control of tomato fruit composition in the resequencing era
auteur
Laura Pascual, Christopher Sauvage, Guillaume Bauchet, Nelly Desplat, Jean-Paul Bouchet, Dominique D. Brunel, Marie-Christine Le Paslier, Mathilde M. Causse
article
11. Solanaceae conference SOL 2014, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). BRA., Nov 2014, Bahia, Brazil
resume
Identifying the genes controlling the variation of quantitative traits is a key goal for geneticists. Genetic loci underlying quantitative trait variation (QTL) have been mapped by traditional linkage mapping for years and positional cloning identified several QTLs. However linkage mapping is limited to the analysis of traits differing between two lines and the impact of genetic background on QTL effect has been underlined.Thanks to the increase in molecular markers, genome-wide association studies were then proposed to circumvent QTL limitations. ln tomato, a self-pollinated crop, we have shown that association studies are possible, using the admixed nature of cherry tomato genomes that limits the impact of population structure in such an approach. Nevertheless, the results might be restricted by linkage disequilibrium and allele frequency, which vary greatly along the genome. Multi-allelic Advanced Generation Inter-Cross (MAGIC) populations allow a wide range of variability to be analyzed and avoid dealing with population structure.
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Conference papers
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https://hal.inrae.fr/hal-02741967/file/Sauvage_Causse%202014%20SOL2014%2032_1.pdf BibTex
titre
Overview of INRA researches on peach brown rot. First results, current works and prospects
auteur
Bénédicte Quilot-Turion
article
COST FA 1104 "4th Management Committee and all Working Group Meeting"_"Sustainable production of high-quality cherries for the Europe market", Oct 2014, Bordeaux, France
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titre
Un modèle intégré écophysiologique-génétique pour l'exploration d'idéotypes: réflexions à partir du cas des monilioses chez la pêche
auteur
Bénédicte Quilot-Turion, Michel M. Génard, Mohamed Ould Sidi
article
Séminaire-Table ronde AGAP, Oct 2014, Montpellier, France
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titre
Deciphering the genetic control of tomato fruit quality in the resequencing era
auteur
Laura Pascual, Christopher Sauvage, Guillaume Bauchet, Nelly Desplat, Jean-Paul Bouchet, Dominique D. Brunel, Marie-Christine Le Paslier, Mathilde M. Causse
article
3. annual conference of the COST ACTION FA 1106, Mediterranean Agronomic Institute. GRC., Sep 2014, Chania, Greece
resume
Identifying the genes controlling the variation of quantitative traits is a key goal for breeders. Genetic variations underlying quantitative traits (QTL) have been mapped by traditional linkage mapping for years and positional cloning identified several QTLs. However linkage mapping is limited to the analysis of traits differing between two lines and the impact of genetic background on QTL effect has been underlined. Thanks to the increase in molecular markers, genome-wide association studies were then proposed to circumvent QTL limitations. ln tomato, a self-pollinated crop, we have shown that association studies are possible, using the admixed nature of cherry tomato genomes that limits the impact of population structure in such an approach. Nevertheless, the results might be limited by linkage disequilibrium, which varies greatly along the genome. Multi-allelic Advanced Generation Inter-Cross (MAGIC) populations allow a wide range of variability to be analyzed and avoid dealing with population structure.
typdoc
Conference papers
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https://hal.inrae.fr/hal-02744187/file/Sauvage_Causse%202014%20Cost%20Action%2044_1.pdf BibTex
titre
Snapmelon (Cucumis melo L. Momordica Group), an indigenous cucurbit from India with immense value for breeding
auteur
N.P.S. Dhillon, H. Singh, Michel Pitrat, Antonio J. Monforte, James D. Mccreight
article
congrès ISHS 2014 « Sustaining Lives, Livelihoods and Landscapes », International Society for Horticultural Science (ISHS). INT., Aug 2014, Brisbane, Australia
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https://hal.inrae.fr/hal-02741451/file/Pitrat%202014%20ISHS_1.pdf BibTex
titre
An ecological multi-level theory of competition for resources used to analyze density-dependence effects in fruit production
auteur
Michel Génard, Zhanwu Dai, Marion Prudent, Bénédicte Quilot-Turion, Francoise Lescourret
article
29. International Horticultural Congress (IHC2014), International Society for Horticultural Science (ISHS). INT., Aug 2014, Brisbane, Australia
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titre
An ecological multi-Level theory of competition for resources used to analyse density-dependence effects in fruit production
auteur
Michel Génard, Francoise Lescourret, Zhanwu Dai, Bénédicte Quilot-Turion, Marion Prudent
article
29. International Horticultural Congress on Horticulture - Sustaining Lives, Livelihoods and Landscapes (IHC) / Int Symposia on the Physiology of Perennial Fruit Crops and Production Systems and Mechanisation, Precision Horticulture and Robotics, Aug 2014, Brisbane, Australia. ⟨10.17660/ActaHortic.2016.1130.6⟩
resume
Lescourret and Génard (2003) developed a multi-level theory of competition for resources applied to fruit production, considering that any collection of unit parts (cells or seeds in a fruit, fruits in an infructescence or in a tree...) can form a population and the population is subject to competition, whatever the level of organization. The principles of the theory are that the mass of each unit decreases when the number of units in the population increases and that the total mass of the population increases as the number of units increases until it reaches a maximum, after which it decreases. A three-parameter model based on that theory was used to analyse the level of density-dependence, i.e., the effect of the population size on its mass. Comparing the number of cells and the mean cell volume in mesocarp of fruits from peach genotypes showed a strong and undercompensating density-dependence that revealed competition between cells. Similarly, when comparing populations for different fruit species, similar results or exact compensation were observed for several levels (fruit, infructescence, tree…). Because undercompensating or exact density-dependence were found in most of the cases, a simpler two parameter model was proposed by Prudent et al. (2013). This two parameter model was used to analyse the genetic control of competition between seeds in multi-seeded fruits.
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titre
The control of soil borne vascular diseases: Limits of genetic resistance of cultivars and rootstocks for controlling Fusarium oxysporum f. sp. melonis (melon) and Verticillium sp. (eggplant)
auteur
F. Villeneuve, F. Latour, T. Thery, Christian Steinberg, Véronique Edel-Hermann, Michel Pitrat, M-Christin Daunay
article
8. International Symposium on Chemical and Non-Chemical Soil and Substrate Disinfestation, Jul 2014, Turin, Italy
resume
After using resistant cultivars or resistant rootstocks for many years to control soil-borne pathogenic fungi causing wilt disease, growers observe more and more frequently wilt like symptoms in their melon and eggplant cultures. Ctifl, in close collaboration with INRA, regional extension services, and seed companies, set up a study designed to investigate the causative pathological agents. The results show the prevalence of Fusarium oxysporum f. sp. melonis race 1-2 yellowing on melon and of Verticillium dahlia on eggplant. Both pathogens are often found together with other pathogenic soil fungi, which act as aggravating factors. The isolates of F. oxysporum f. sp. melonis collected from infected plants appear to be more aggressive than the reference strains currently used for screening resistant material in breeding programmes. In the case of Verticillium wilt, the taxonomic and race status of the isolates sampled, remain unclear
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titre
Quantification de l’intensité de l’activité biologique dans les sols par l’analyse d’images de lames minces de sol : mise au point méthodologique et quantification de l’impact de 250 ans de mise en culture
auteur
Manon Bajard, David Montagne, Jean-Marc Gilliot, Ophélie Sauzet, Mathieu Cammas
article
12ème Journées Nationales d’Etude des Sols. Le sol en héritage, Jun 2014, Bourget du Lac, France
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https://hal.science/hal-01820570/file/Actes-JES-2014-Session4.pdf BibTex
titre
lnsights into the genetic control of resistance to Aphis gossypii in melon: what we learned from the diversity of aphids
auteur
Nathalie Boissot, Sophie Thomas, Pascale Mistral, Virginie Chareyron, Vincent Rittener
article
2.congrès International Hemipteran-Plant Interactions Symposium. HPIS 2014, Jun 2014, Riverside, United States
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https://hal.inrae.fr/hal-02739307/file/Boissot_Thomas%202014%20HPIS%2027_1.pdf BibTex
titre
Variability of the genetic response of tomato during soil drying and repeated cycles of water deficit and recovery
auteur
Julie Ripoll, Laurent Urban, Béatrice Brunel, Alain Goujon, Jean-Claude L'Hôtel, Mathilde M. Causse, Nadia Bertin
article
Plant Biology Europe FESPB/EPSO Congress 2014, Jun 2014, Dublin, Ireland
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https://hal.inrae.fr/hal-02795616/file/Poster%20Ripoll%20Julie%201_1.pdf BibTex
titre
Protection of the durability of major resistance genes to plant viruses through quantitative resistance, a modeling approach
auteur
Elsa Rousseau, Jérôme Coville, Frédéric Fabre, Ludovic Mailleret, Alain Palloix, Benoît Moury, Frédéric Grognard
article
ECMTB14 - 9. European Conference on Mathematical and Theoretical Biology, Jun 2014, Goteborg, Sweden
resume
The deployment of virus-resistant crops often leads to the emergence of resistance-breaking (RB) pathogens that suppress the yield benefit provided by the resistance [1]. Although break- downs are well known for plant genes conferring total, i.e. qualitative resistance to the virus, they are still poorly understood for plant genetic factors conferring partial, i.e. quantitative resistance. Furthermore, it has been proved for several pathosystems that combining qualita- tive and quantitative resistances can increase the durability of the qualitative resistance [2, 3]. Two mechanisms can explain this result : either (i) an increase of genetic drift in the virus population, or (ii) a decrease of the selection advantage of the RB mutant. Here, we aim at disentangling the role of these two mechanisms on the durability of plant qualitative resistances to viruses.
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Conference papers
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https://hal.inrae.fr/hal-02739537/file/2014_Rousseau_ECMTB14_1.pdf BibTex
titre
Tomato variation, origin and domestication
auteur
Jose Miguel Blanca, J. Monterau-Pau, Christopher Sauvage, Guillaume Bauchet, E. Illa, Monique Diez, Mathilde M. Causse, D. Francis, Joaquin Canizares, E. van Der Knaap
article
XVIII. Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 1 p
resume
A collection of 949 accessions composed by S. lycopersicum var. lycopersicum (SLL; 514), S. l. var. cerasiforme (SLC; 291), S. pimpinellifolium (SP; 128), S. galapagense (SG;3), S. neorickii (SN; 1), S. chmielewski (Schm; 1) and 11 crosses between SP, SLC, SLL and S. pennellii was analyzed. The genotyping was carried out using the 7720 markers of the SolCAP genotyping platform and some key genes involved in the domestication process (CNR/FW2.2, SlKLUH/FW3.2, LC, FAS, OVATE and SUN). Using Principal Component Analyses (PCAs) based on the genetic data a new genetic-based classification divided the accessions in SP (121), SLC (236), SLL (492) and mixture (100). Within SP and Andean SLC a complex geographical structure correlated with the climate was found. Ecuadorian SP was remarkably differentiated from the rest of SP. In non-Andean SLC the structure found was not as marked. In a neighbor phylogenetic network SLC appeared intermediate between SP and SLC. Thus SLC was found to be a semi-domesticate with a key role in the tomato domestication. Andean SLC had a diversity comparable to SP while the non-Andean SLC had a lower diversity comparable to vintage SLL, indicating that the main bottleneck in the tomato domestication happened in the migration from the Andean region to Mesoamerica. Most SLC accessions were reported to have cherry-sized fruit, but interestingly some South American SLC were collected in markets as full-sized vintage tomatoes. Thus the vintage tomato in South America included SLC as well as SLL materials.
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https://hal.inrae.fr/hal-02796249/file/Blanca%20Jose_%20T001_Eucarpia%20tomato%202014_1.pdf BibTex
titre
A multilocus mixed model for GWAS reveals associations for metabolic traits in the tomato, [i]Solanum lycopersicum[/i]
auteur
Christopher Sauvage, Vincent Segura, Guillaume Bauchet, Rebecca Stevens, P.T. Do, A. Fernie, Mathilde Causse
article
XVIIIth Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 1 p
resume
absent
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https://hal.science/hal-01268525/file/Sauvage%20Christopher_P015_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Deciphering ascorbic acid regulation in tomato fruit ripening
auteur
Celine Bournonville, Carine Ferrand, Daniel Just, Virginie V. Garcia, Daniel Jacob, Rebecca Stevens, Hélène Gautier, Christophe Rothan, Pierre Baldet
article
18. EUCARPIA Meeting of the Tomato Working Group, Apr 2014, Avignon, France. 1 p
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https://hal.inrae.fr/hal-02800985/file/Stevens%20Rebecca_%20T021_Eucarpia%20tomato%202014_1.pdf BibTex
titre
The study of domestication reveals a loss of diversity, signature of selection and transcriptional modifications in tomato (Solanum lycopersicum)
auteur
Christopher Sauvage, Benoit Nabholz, S. Gautier, M. Ruiz, Mathilde M. Causse, J. David, S. Glemin
article
XVIIIth Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 1 p
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https://hal.inrae.fr/hal-02794508/file/Sauvage%20Christopher%20_%20T004_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Metabolic profiling of transgenic lines silenced for an MDHAR gene and impact on ascorbate redox state and carbon metabolism
auteur
Vincent Truffault, Guy Costagliola, Hélène Gautier, Rebecca Stevens
article
18. EUCARPIA Meeting of the Tomato Working Group, Apr 2014, Avignon, France. 1 p
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https://hal.inrae.fr/hal-02799822/file/Stevens%20Rebecca%20_%20T009_Eucarpia%20tomato%202014_1.pdf BibTex
titre
The Tomato-Potyvirus system, a comparative system for natural and induced resistances associated with the initiation factors 4E
auteur
Camille Gauffier, Caroline Lebaron, André Moretti, C. Constant, F. Moquet, G. Bonnet, Carole Caranta, Jean-Luc J.-L. Gallois
article
XVIIIth Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 1 p
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Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02798800/file/Gauffier%20Camille%20_%20T017_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Protecting the durability of major resistance genes to plant viruses with quantitative resistance
auteur
Elsa Rousseau, Frédéric Fabre, Ludovic Mailleret, Alain Palloix, Vincent Simon, Sophie Valière, Benoît Moury, Frédéric Grognard
article
Conférence Jacques Monod, Apr 2014, Roscoff, France
resume
The deployment of virus-resistant crops often leads to the emergence of resistance-breaking (RB) pathogens that suppress the yield benefit provided by the resistance (Fabre et al,2012). Although breakdowns are well known for qualitative resistances conferring total resistance to the virus, they are still poorly understood for quantitative resistances conferring partial resistance. Furthermore, it has been proved for several pathosystems that combining qualitative and quantitative resistances can increase the durability of the qualitative resistance (Palloix et al, 2009, Quenouille et al, 2013). Two mechanisms can explain this result : either (i) an increase of genetic drift in the virus population, or (ii) a decrease of the selection advantage of the RB mutant. The purpose of the ongoing work presented here is to disentangle the role of these two mechanisms on the durability of plant qualitative resistances to viruses.To investigate this, we concentrated on a pepper Potato virus Y (PVY) pathosystem on which we report consistent variability in bottlenecks undergone by viral populations at inoculation and in viral accumulation, both determined by the host genotype. We follow by high-throughput sequencing the within-host demo-genetic dynamics of several virus variants in contrasted plant genotypes, carrying a qualitative resistance and different combinations of quantitative resistances. The respective effects of genetic drift and selection are evaluated by fitting models to these data (Fabre, 2012). We further aim at predicting the potential of combining qualitative and quantitative plant resistances to achieve sustainable plant resistance management at the epidemic scale.
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titre
From genetics to marketing (and through hidden connexions and interdependencies): an integrative approach of the ecologisation of fruit production
auteur
Claire Lamine, Jean Marc Audergon, Servane Penvern, Sylvaine S. Simon, Jean Pluvinage
article
11. European IFSA Symposium: Farming systems facing global challenges: Capacities and strategies., Apr 2014, Berlin, Germany. 143 p
resume
This paper discusses a systemic analysis of the changes in the peach and apricot industry since the 1960s, based on a sociological framework anchored in transition and actor network theories, with a specific focus on the place of genetics and breeding strategies in this trajectory, an aspect which indeed is often neglected in the literature. We show how, a long with a process of specialization and intensification of the fruit production at the farm and regional levels, breeding strategies mainly focus on improving yield and technological fruit quality and on extending the production period (from 3 to nearly 6 months for peach production) to offer retailers a continuity in each product type over this entire extended season through “varietal series”. These objectives are thus market-driven and neglect the environmental aspects (even though substantial work has been done for decades on resistant cultivars) and the fruit production constraints, and they lead to create and then market fruits that are easy to store and transport, but often disappointing in terms of taste and quality. These changes are due to the constraints which affect all the socio-technical system up to the breeding process criteria and to a deep reconfiguration of the network of institutions and actors dealing with fruit production in general (research institutes, breeders, producers’ organizations, retailers, etc.). In this paper, we also try to adopt a reflexive perspective about the way we discuss with the projects’ members and partners (researchers from various disciplinary backgrounds, producers’ organizations, marketers and advisors) some key issues that are central to the construction of more ecological and resilient transition pathways at the scale of the agrifood system.
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titre
Conservation and use of natural genetic variation of eggplant in the genomics Era
auteur
J. Prohens, Marie-Christine Daunay, S. Knapp, R. Meyer, S. Vilanova
article
Applied Vegetable Genomics, Feb 2014, Vienne, Austria. 1 p
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Conference papers
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https://hal.inrae.fr/hal-02794179/file/Daunay%202014%20Applied%20vegetable%20genomics%2011_1.pdf BibTex
titre
Variation and origin of the tomato
auteur
J. Blanca, J. Montero-Pau, Christopher Sauvage, Guillaume Bauchet, E. Illa, M. Diez, D. Francis, Mathilde M. Causse, E. van Der Knaap, Joaquin Canizares
article
Applied Vegetable Genomics, Vienna Institute for Nature Conservation and Analyses (VINCA). AUT., Feb 2014, Vienne, Austria. pp.12
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Conference papers
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https://hal.inrae.fr/hal-02739830/file/Sauvage_Bauchet%202014%20Applied%20vegetable%20genomics%2012_1.pdf BibTex
titre
Valorisation of the natural variation available in tomato accessions to decipher the genetic control of fruit quality traits
auteur
Laura Pascual-Banuls, Jiaxin Xu, Christopher Sauvage, Guillaume Bauchet, Nelly Desplat, Jean-Paul Bouchet, Dominique D. Brunel, Mathilde M. Causse
article
Applied Vegetable Genomics, Feb 2014, Vienne, Austria
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Conference papers
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https://hal.inrae.fr/hal-02741808/file/OK%20Sauvage_Bauchet%202014%20Applied%20vegetable%20genomics%2020_1.pdf BibTex
titre
Reconstruction bayésienne d’arbres de transmission à partir de données épidémiologiques et génétiques
auteur
Gael Thébaud, M. J. Morelli, Joel J. Chadoeuf, Donald Peter King, Daniel T. Haydon, Samuel S. Soubeyrand
article
Réunion du Réseau Ecologie des Interactions Durables, Feb 2014, Montpellier, France
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titre
Effet des génotypes des plantes sur l’efficacité des Stimulateurs de Défenses des Plantes (SDP) : exemples des couples oïdium/melon et mildiou/laitue
auteur
Brigitte Maisonneuve, Caroline Allen-Aubouard, Michel Pitrat
article
SIVAL, Jan 2014, Angers, France
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titre
Genome wide sequence-based association studies for dissection of important agronomie traits in apricot
auteur
Véronique Decroocq, Stéphanie Mariette, Fabienne Wong Jun Tai, Stephane Decroocq, David Tricon, Patrick Lambert, Guillaume Roch, Jean Marc Audergon, Macha Nicholski, Alexis Groppi, Aurélien Barré, Filippo Geuna, Daniele Bassi, Stefano Tartarini, Luca Dondini, Tatyana Zhebentyayeva, Albert Abbott
article
Plant and Animal Genome XXII Conference, Jan 2014, San Diego, United States
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Conference papers
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https://hal.inrae.fr/hal-02796089/file/Lambert_Roch%202014%20Plant_1 BibTex
titre
Control of chilling requirement and flowering time in trees; linking genetics and genomics to understand these complex phenological traits in deciduous fruit and forest trees
auteur
Albert G. Abbott, Stéphanie Mariette, Lucia Andreini, Tatyana Zhebentyayeva, Zongrang Liu, Guillaume Roch, David Tricon, Patrick Lambert, Jean Marc Audergon, Macha Nicholski, Alexis Groppi, Aurélien Barré, Catherine Bodenes-Brezard Bodénès, Grégoire G. Le Provost, Christophe Plomion, Antoine Kremer, Christopher Dardick, Ralph Scorza, Fred Hebard, C. Dana Nelson, Scott Merkle, Campbell J. Nairn, Véronique Decroocq
article
Plant and Animal Genome XXII Conference, Jan 2014, San Diego, United States
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https://hal.inrae.fr/hal-02798021/file/Roch_Lambert%202014%20Plant_1 BibTex

Other publications

titre
Légumes : l’innovation pour tous
auteur
Michel Pitrat
article
2014, pp.53-55
resume
Résistance aux bio-agresseurs, diversification, qualité, rusticité, agriculture biologique sont les principales innovations marquantes de ces dernières années en matière d’espèces légumières. L’amateur, qui n’a pas les mêmes exigences que le professionnel, n’est pas oublié...
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https://hal.science/hal-01312110/file/2014_Pitrat_Jardins%20de%20France%20629_1.pdf BibTex
titre
Le melon, toute une histoire !
auteur
Michel Pitrat
article
2014
resume
Membre de la famille des cucurbitacées, le melon ([i]Cucumis melo[/i]) est proche parent du concombre ([i]Cucumis sativus[/i]) et plus éloigné de la pastèque ([i]Citrullus lanatus[/i]). Il est relativement facile de différencier sur les étalages en France ces trois espèces, en particulier la pastèque avec les graines réparties dans la chair et non regroupées dans une cavité centrale. Cependant, au cours de voyages à l’étranger, on peut avoir quelques difficultés à s’y reconnaître. Par ailleurs, les documents anciens ne permettent pas toujours de distinguer ces trois espèces. C’est dans un petit voyage dans le temps et dans l’espace que nous vous convions autour de la diversité biologique du melon.
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https://hal.science/hal-01312109/file/2014_Pitrat_Jardins%20de%20France%20627_1.pdf BibTex
titre
Gérer les épidémies virales en combinant résistances aux virus et résistances aux pucerons : quel intérêt ?
auteur
Nathalie Boissot, Alexandra Schoeny, Pauline Millot, Catherine Wipf-Scheibel, Karine Nozeran, Hervé Lecoq, Frédéric Fabre
article
2014, pp.127-136. ⟨10.15454/1.462267742270331E12⟩
resume
Host plant resistance made it possible to reduce impact of virus diseases in numerous crops. Host plant resistance can either directly target viruses or target their vectors, mainly aphids. In this study, we showed that the melon resistance to the aphid Aphis gossypii reduces incidence of two viruses, CABYV, transmitted on a persistent mode, and CMV, transmitted on a non-persistent mode. Next, we investigated using mathematical modeling the potential of jointly deploying resistance to aphids and to viruses to enhance control of viral epidemics. Various agro-ecological systems were considered, mimicking the situation of melon crops in South-East of France. If the benefits of such a deployment are highly probable in the case of CABYV, they remain largely uncertain (but in no case negative) for the control of CMV.
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https://hal.inrae.fr/hal-02995948/file/2015_Boissot_Innov%20Agron.pdf BibTex

Poster communications

titre
Evaluation of Turkish melon accessions for resistance to Fusarium wilt, downy mildew, powdery mildew, Cucumber mosaic virus and Zucchini yellow mosaic virus
auteur
Ilkour Solmaz, N. Sari, Catherine Dogimont, Michel Pitrat
article
29. International Horticultural Congress on Horticulture - Sustaining Lives, Livelihoods and Landscapes (IHC) / International Symposium on Plant Breeding in Horticulture, Aug 2014, Brisbane, Australia. Acta Horticulturae, 1127, 2016, Acta Horticulturae. ⟨10.17660/ActaHortic.2016.1127.22⟩
resume
The aim of this study was to detect disease resistance in melon genetic resources collected from different regions (south-eastern Anatolia, middle Anatolia, Aegean, Marmara-Thrace and eastern Anatolia) of Turkey. A total of 85 melon and 15 snake melon genotypes were tested against Fusarium wilt (races 1 and 2), downy mildew and powdery mildew (race 3) as fungal diseases, and Cucumber mosaic virus (CMV) and Zucchini yellow mosaic virus (ZYMV) as virus diseases. In the case of Fusarium wilt race 1, eight genotypes were found resistant, 86 genotypes were susceptible and one genotype was segregating. Fifty-four percent of the genotypes were found to be resistant, 28% were susceptible and 18% were segregating for Fusarium wilt race 2. In the case of downy mildew, four genotypes (‘Kav 28’, ‘Kav 71’, ‘Kav 237’ and ‘Kav 255’) were resistant, while 78 genotypes were susceptible and 16 genotypes were segregating. Among all the genotypes tested, only two genotypes (‘Kav 60’ and ‘Kav 277’) were resistant to powdery mildew. While two genotypes (‘Kav 281’ and ‘Ac 33’) were found to be resistant to CMV, none of the genotypes were resistant to ZYMV.
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Poster communications
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https://hal.science/hal-01604952/file/Dogimont_Pitrat%20Acta%20Horticulturae%202016%20133-140_1.pdf BibTex
titre
New advances on the flesh's red pigmentation in peach
auteur
Thierry Pascal, Sylvie Bureau, Laure Heurtevin, Jehan-Baptiste Mauroux, Carole C. Confolent, Patrick Lambert, Virginie Diévart, Christophe Tuero, Jean-Luc Poessel, Zhijun Shen
article
7. International Rosaceae Genomics Conference, Jun 2014, Seattle, United States. 2014
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Poster communications
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https://hal.inrae.fr/hal-02743461/file/Pascal%202014%20RGC7%2087_1.pdf BibTex
titre
Identification and characterization of QTLs for peach fruit quality by pedigree-based analyses (PBA) in the FruitBreedomics project
auteur
Jose Ramon Hernandez, Diego Mlcheletti, Pere Arús, Teresa Barreneche, Daniele Bassi, Marco Bink, Maria Teresa Dettori, Elisabeth Dirlewanger, Patrick Lambert, Francois Laurens, Sabrina Micali, Thierry Pascal, Bénédicte Quilot-Turion, Laura Rossini, Michela Troggio, Verde Lgnazio, Eric van de Weg, Maria José Aranzana
article
7. International Rosaceae Genomics Conference, Jun 2014, Seattle, United States. 2014
resume
absent
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.science/hal-01209974/file/OK%20Lambert_Pascal%202014%20RGC7%20101_1.pdf BibTex
titre
Identification by a RNAseq approach of virulence gene candidates in the non-model species Aphis Gossypii
auteur
Leslie Dutartre-Fricaux, Jean-Paul Bouchet, Coralie Beucher, Nathalie Boissot, Catherine Dogimont
article
2. International Hemipteran-Plant Interactions Symposium. HPIS 2014, Jun 2014, Riverside, United States. 2014
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Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02801072/file/Boissot_Dogimont%202014%20HPIS%2056_1.pdf BibTex
titre
Differential effect of resistance inducers on the susceptibility of lettuce varieties to Sclerotinia sclerotiorum and Botrytis cinerea
auteur
Asti Irawanti Azis, Magali Duffaud, Claire Troulet, Brigitte B. Maisonneuve, Efi Toding Tondok, Suryo Wiyono, Marc Bardin, Philippe C. Nicot
article
13. IOBC-WPRS Meeting of the working group "Biological control of fungal and bacterial plant pathogens". Biocontrol of plant diseases: "from the field to the laboratory and back again", Jun 2014, Uppsala, Sweden. 2014
resume
The preventive use of resistance inducers has been shown to be an interesting method to reduce dependency on pesticides for plant protection. However, little is known on possible differences in the protective effects of such methods for different varieties of a given crop. In the present study, we assessed the effect of three compounds (acibenzolar-S-méthyl, a calcium-based mineral compound and a yeast extract) for the protection of six varieties of lettuce against two major pathogens, Sclerotinia sclerotiorum and Botrytis cinerea. The compounds were sprayed on the plants three days before inoculation. A water spray was used as a control. The protective effect of the compounds was then assessed by comparing the size of lesions developing on inoculated leaves. For both pathogens, none of the compounds fully inhibited disease development. However, reduction in lesion size was observed on some of the leaves. The effect of the three compounds was different for the two pathogens. For tests with B. cinerea, effects of plant treatment were not statistically significant. In contrast, significant effects were found for five of the varieties inoculated with S. sclerotiorum. Overall, the yeast extract provided the highest level of protection against that pathogen. However, for all compounds, the extent of the protective effect depended on the variety. Furthermore, in some cases the effect of the compound was opposite to that desired and disease was more severe on treated plants than on the water control. Possible consequences for field application of such methods will be discussed.
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Poster communications
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https://hal.inrae.fr/hal-02740639/file/2014-Azis_IOBC_1.pdf BibTex
titre
Regulation of tomato ascorbate levels: a candidate gene involved in fruit nutritional value and stress tolerance
auteur
Noé Gest, Vincent Truffault, Hicham El Airaj, Cecile Garchery, Gisèle Riqueau, Mathilde M. Causse, Hélène Gautier, A. Jimenez, Rebecca Stevens
article
XVIIIth Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 1 p., 2014
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Poster communications
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https://hal.inrae.fr/hal-02796653/file/Stevens%20Rebecca_P007_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Deciphering the genetic control of quantitative traits with magic
auteur
Laura Pascual-Banuls, Nelly Desplat, Jean-Paul Bouchet, Aurore Desgroux, Laure Bruguier, B. Chauchard, P. Verschave, Mathilde Causse
article
XVIIIth Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 2014
resume
absent
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.science/hal-01208696/file/Causse%20Mathilde_%20T028_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Combining deficit irrigation and recovery periods can save water and maintain yield and fruit quality in tomato
auteur
Julie Ripoll, Laurent Urban, Béatrice Brunel, Alain Goujon, Jean-Claude L'Hôtel, Mathilde M. Causse, Nadia Bertin
article
18. EUCARPIA Meeting of the Tomato Working Group, Apr 2014, Avignon, France. 2014
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Poster communications
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https://hal.inrae.fr/hal-02743459/file/PosterEucarpia2014_1.pdf BibTex
titre
GWAS reveals candidate regions for metabolic traits in tomato, Solanum lycopersicum
auteur
Guillaume Bauchet, S. Grenier, N. Samson, J. Bonnet, L. Grivet, E. Bolygo, C. Baxter, Mathilde M. Causse
article
XVIIIth Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 1 p., 2014
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Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02800694/file/Bauchet%20Guillaume_%20T024_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Promote adaptation to drought and fruit quality by alternating increased intensity of deficit irrigation and recovery period
auteur
Julie Ripoll, Laurent Urban, Béatrice Brunel, Alain Goujon, Jean-Claude L'Hôtel, Mathilde M. Causse, Nadia Bertin
article
XVIII. Eucarpia Meeting, Vegetable section, Tomato Working group, Apr 2014, Avignon, France. 2014
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02743661/file/OK%20Causse%20Mathilde_P006_Eucarpia%20tomato%202014_1.pdf BibTex
titre
Protecting the durability of major resistance genes to plant viruses with quantitative resistance
auteur
Elsa Rousseau, Frédéric Fabre, Ludovic Mailleret, Alain Palloix, Vincent Simon, Sophie Valière, Benoît Moury, Frédéric Grognard
article
Conférences Jacques Monod : “From emerging to pandemic viruses: interplay between host ecology and viral evolution”, Apr 2014, Roscoff, France. , 2014
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Poster communications
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https://hal.inrae.fr/hal-02738827/file/2014_Rousseau_Conf%20Monod_poster_1.pdf https://hal.inrae.fr/hal-02738827/file/2014_Rousseau_Conf%20Monod_2.pdf BibTex
titre
Genome wide associations for metabolic traits in tomato
auteur
Guillaume Bauchet, S. Grenier, N. Samson, J. Bonnet, L. Grivet, E. Bolygo, C. Baxter, Mathilde M. Causse
article
Applied Vegetable Genomics, Feb 2014, Vienne, Austria. pp.28, 2014
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02740557/file/Bauchet_Causse%20Applied%20vegetable%20genomics%2028_1.pdf BibTex
titre
Étude du rôle des gènes Mlo dans la résistance aux oïdiums du melon
auteur
Sonia Elbelt, Nathalie Giovinazzo, Verane Sarnette, Christelle Troadec, Abdelhafid A. Bendahmane, Catherine Dogimont
article
10. Rencontres de Phytopathologie-Mycologie de la Société Française de Phytopathologie (SFP), Journées Jean Chevaugeon 2014, Jan 2014, Aussois, France. 2014
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Poster communications
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https://hal.inrae.fr/hal-02800948/file/Elbert_Dogimont%202014%2010.%20renc.%20phyto-myco%20Poster_1.pdf BibTex

Reports

titre
Clonage des gènes effecteurs de phytophtora capsici pour guider le développement de variétés résistantes de poivrons
auteur
Véronique Lefebvre
article
[Contrat] Association Provençale de Recherche et d'Expérimentation Légumière (APREL); University of Tennessee; The James Hutton Institute. 2014
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titre
Valorisation des ressources génétiques et génomiques de la tomate pour l'amélioration de la qualité des fruits
auteur
Mathilde M. Causse
article
[Contrat] Institut National de la Recherche Agronomique (INRA); Vilmorin. 2014
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2013

Conference papers

titre
Point sur les projets labellisés. DEFILeg (2010-2013) : Alternatives aux pesticides dans une gestion durable de la protection des cultures,
auteur
Brigitte Maisonneuve
article
6. Rencontres du GIS PIClég, Groupement d'Intérêt Scientifique pour la Production Intégrée en Cultures légumières (Gis Piclèg). FRA., Dec 2013, Paris, France
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titre
LactuMel (2014-2016) : Recherche de résistance aux nématodes à galles chez la laitue
auteur
Brigitte Maisonneuve
article
6. Rencontres du GIS PIClég, Groupement d'Intérêt Scientifique pour la Production Intégrée en Cultures légumières (Gis Piclèg). FRA., Dec 2013, Paris, France
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titre
Iconographie du Moyen Age à la renaissance: Contribution à l'histoire des plantes (Solanacées, curcubitacées)
auteur
Marie-Christine Daunay, Jules Janick, Harry S. Harris
article
Journées d'étude de l'Inst. Nat. d'Hist.de l'Art (Paris) et du Potager du Roi (Versailles), Entre jardin et table: regards sur la nature morte des fruits et légumes, nouvelles approches du vivant, Nov 2013, Paris, France
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titre
How a species becomes polyploid – The Brassica model
auteur
Anne-Marie Chèvre, Frederique Eber, Maryse Lodé-Taburel, Gwenn Trotoux, Virginie Huteau, Olivier Coriton, Cyril Falentin, Gilles Lassalle, Emmanuel Szadkowski, Maria Loreto Suay Llopis, Maria M. Manzanares-Dauleux, Régine Delourme, Sophie Paillard, Eric Jenczewski, Karine Alix-Jenczewski
article
Colloque de Génomique Environnementale : Diversité, Evolution et Fonctionnement du Vivant à l’Ere des Nouvelles Technologies de Séquençage, Nov 2013, Rennes, France
resume
How a species becomes polyploid – The Brassica model. Colloque de Génomique Environnementale : Diversité, Evolution et Fonctionnement du Vivant à l’Ere des Nouvelles Technologies de Séquençage
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titre
The GEDUNEM project: Varietal and technical innovations for the sustainable and integrated management of root-knot nematodes in protected vegetable cropping systems
auteur
Mireille Navarrete, Caroline Djian-Caporalino, Mathilde Chapuis, Marc Tchamitchian, Arnaud Dufils, Amélie Lefevre, Laure Pares, Thierry Mateille, Alain Palloix, Hélène Védie, Claire Goillon, Ariane Fazari, Nathalie Marteu, Philippe Castagnone-Sereno
article
2. International Symposium on Organic Greenhouse Horticulture, International Society for Horticultural Science (ISHS). Leuven, INT., Oct 2013, Avignon, France. 6 p
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titre
New resistance-genes deployment strategies as non chemical alternatives for the durable management of root-knot nematodes in vegetable crops rotation
auteur
Caroline Djian-Caporalino, Alain Palloix, Ariane Fazari, Nathalie Marteu, Arnaud Barbary, Anne-Marie Sage-Palloix, Sabine Risso, Roger Lanza, Catherine Taussig, Philippe Castagnone-Sereno
article
2nd international symposium on organic greenhouse horticulture, International Society for Horticultural Science (ISHS). INT., Oct 2013, Avignon, France. 310 p
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Conference papers
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https://hal.inrae.fr/hal-02744854/file/Palloix%20_2013_%20ISHS-1_1.pdf https://hal.inrae.fr/hal-02744854/file/Palloix%20_2013_%20ISHS-1_2.doc BibTex
titre
Combining systems biology, genome resequencing and MAGIC population to decipher the genetic variation of tomato fruit quality
auteur
Laura Pascual, Jiaxin Xu, Nelly Desplat, Yves Y. Gibon, Annick Moing, Mickael M. Maucourt, Stéphane Bernillon, Catherine Deborde, Jean-Paul Bouchet, Dominique D. Brunel, Marie-Christine Le Paslier, Mathilde M. Causse
article
10. Solanaceae conference (SOL 2013), Oct 2013, Beijing, China
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Conference papers
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https://hal.inrae.fr/hal-02750138/file/SOL2013_combinig-systems-biology_1.pdf BibTex
titre
Towards a better interaction between european Solanaceae germplasm holders and Solanaceae omics researchers
auteur
Marie-Christine Daunay, Willem van Dooijeweert, Roel Hoekstra, Lukas A. Mueller
article
10. Solanaceae Conference: “geno versus pheno”, Oct 2013, Beijing, China
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Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02804268/file/Daunay%202012%20SOL%20newsletter%201-16_1.pdf BibTex
titre
Early history and iconography of the Solanaceae: 3. Tomato
auteur
Marie-Christine Daunay, Jules Janick
article
10. Solanaceae Conference: “geno versus pheno", Oct 2013, Beijing, China
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Conference papers
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https://hal.inrae.fr/hal-02806135/file/Daunay%202012%20SOL%20newsletter%201-16_1.pdf BibTex
titre
Collaborative work on effectors in Phytophthora capsici
auteur
Véronique Lefebvre, Kurt Lamour
article
6. Effectome meeting, Sep 2013, Lauret, France
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titre
Which strategies to search for avirulence factor in resistance plant/aphid interaction
auteur
Leslie Dutartre, Nathalie Boissot
article
6. Effectome meeting, Sep 2013, Lauret, France
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titre
Influence of ascorbate-recycling, light and temperature during tomato fruit ripening on ascorbate pool and ascorbate-degradation
auteur
Vincent Truffault, Hélène Gautier, Rebecca Stevens
article
11. International POG Conference Reactive Oxygen and Nitrogen Species in Plants, Sep 2013, Varsovie, Poland
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Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02745080/file/Truffault_Stevens%202013%20BioTechnologia%20199_1.pdf https://hal.inrae.fr/hal-02745080/file/Truffault_Stevens%202013%20BioTechnologia%20199_2.docx BibTex
titre
Valorization of the natural variation available in tomato accessions to decipher the genetic control of quantitative traits
auteur
Laura Pascual-Banuls, J. Xu, Christopher Sauvage, Guillaume Bauchet, Nelly Desplat, Jean-Paul Bouchet, D. Bruner, Marie-Christine Le Paslier, Mathilde M. Causse
article
57. Annual Congress SIGA, Sep 2013, Foggia, Italy
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titre
Preserving the efficiency of resistance genes against viruses from the individual host plant to agricultural landscapes
auteur
Benoît Moury, Frédéric Fabre, Alain Palloix
article
EMBO Workshop : Green viruses, from gene to landscape, Sep 2013, Hyères-les-Palmiers, France. 129 p
resume
The emergence of virus variants able to circumvent the resistance mechanisms of host plants, i.e. resistance- ‐breaking variants, is a complex phenomenon that involves different evolutionary forces operating at different spatial scales. Resistance breakdown can be decomposed into three major steps: appearance of resistance- ‐breaking virus variants by mutation, accumulation of these variants in plants in competition with wild- ‐type viruses and spread from plant to plant. Resistance breakdown occurs when these three steps are achieved efficiently. If not, the resistance is said durable. Reverse genetics analyses allowed the identification of the mutational pathways responsible for resistance breakdown and the measure of fitness costs associated to these mutations in a number of resistance gene- ‐virus species pairs. However, predicting the consequences of these genetic parameters at higher scales (crop fields, heterogeneous agricultural landscapes) is far beyond our experimental capacities because of the high number of plant- ‐virus combinations to analyze and because of the high number of factors at play during virus emergences. For this purpose, we developed different mathematical models. An analytical model showed that the evolutionary constraint exerted on virus pathogenicity factors (the factors that are recognized by the plant resistance genes) was a good predictor of the durability of the corresponding plant resistance gene. In another approach, simulation models allowed quantifying the respective roles of genetic, demographic and epidemiological factors affecting viral populations during resistance breakdown and estimating what effects on resistance durability could be expected from different leverages available to plant breeders and growers (choice of resistance genes, distribution of resistant and susceptible cultivars among fields, additional control methods acting on epidemics).
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Origin and diversity of the sources of resistance to PPV in apricot: implications and use in marker-assisted resistance to sharka
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Véronique Decroocq, David Tricon, Stephane Decroocq, Stéphanie Mariette, Sevda Babayeva, Patrick Lambert, Guillaume Roch, Jean Marc Audergon, F. Geuna, Daniele Bassi, Luca Dondini, Stefano Tartarini, Jaroslav Salava, Tatyana Zhebentyayeva, Albert Abbott
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2. International Symposium on Plum Plox Virus, Sep 2013, Olomouc, Czech Republic
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https://hal.inrae.fr/hal-02806628/file/Lambert_Roch%202013%20Int.%20Sym.%20Plum%20Pox%20Virus_1.pdf BibTex
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Potentialities of wild relatives for eggplants and eggplants rootstocks breeding
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Marie-Christine Daunay, F. Bletsos, J.W. Hennart, J.P.W. Haanstra, G. van Der Weerden
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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https://hal.inrae.fr/hal-02748311/file/Daunay%202012%20Eucarpia%20231_1.pdf BibTex
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Evaluation of new resistance-genes deployment strategies in the pepper Capsicum annuum for the durable management of root-knot nematodes
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Caroline Djian-Caporalino, Alain Palloix, Ariane Fazari, Nathalie Marteu, Arnaud Barbary, Anne-Marie Sage-Palloix, S. Risso, R. Lanza, C. Taussig, P. Castagnone-Sereno
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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The current restrictions on the use of chemical nematicides have contributed to increase rootknot nematode problems in horticultural crops. ln this context, plant resistance appears as the most effective method of control, but the possible occurrence of virulent nematodes able to reproduce on R-plants may constitute a severe threat to this control strategy. ln Capsicum annuum, resistance to RKN is controlled by several dominant genes - the N and Me genes. To implement a rational management of the R-lines increasing the durability of the R-genes, we tested several R-gene deployment strategies. Experiments were conducted in climate-controlled roorns, in greenhouses, and under 3-years-field agronomie conditions to compare i) the succession of the same R-genes every year, when introgressed in a partially resistant vs. a susceptible genetic background, ii) the alternance, ii) the mixed cultivation and iv) the pyramiding of two R-genes with different modes of action in a single genotype. At the plant level, we previously showed that the choice of the R-genes and the genetic backgrounds in which they are introgressed can lower the frequency of resistance breakdown, and that the pyramiding of two different R-genes in one genotype totally suppressed the emergence of virulent isolates. Here, at the field and rotation level, we confirmed these results and showed that i) alternating different R-genes in rotation is efficient to reduce the selection pressure of R-genes on the pathogens and allows to recycle broken R-genes, and ii) optimal cultivation practices of R-plants increase their "trap" effect and may decrease the amount of pathogens in the soil, below their damage threshold. These results are in good agreement with concepts recently developed from the analysis of other plant-pathogen interactions. The root-knot nematode model could thus contribute to generalize strategies for the breeding and management of R-cultivars strengthening and increasing the durability of qualitative resistances
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https://hal.inrae.fr/hal-02746488/file/Palloix%202013%20Eucarpia%2037-45_1.pdf BibTex
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Analysis of a complex QTL region controlling the broad-spectrum resistance to Phytophthora capsici root rot by comparative mapping and association study in pepper germplasm
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Véronique Lefebvre, Melissa Cantet, Céline Vandecasteele, Sonia S. Vautrin, Jean-Paul Bouchet, Anne Massire, Alexandre Bachellez, Nasradin Touhami, Stéphanie Mallard, Marie-Christine Le Paslier, Anne-Marie Sage-Palloix, Abdelhafid A. Bendahmane, Alain Palloix, Helene H. Berges, Dominique D. Brunel
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15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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https://hal.inrae.fr/hal-02750004/file/Lefebvre_Cantet%202013%20Eucarpia%20139-146_1.pdf BibTex
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Peppers and Potyviruses, a pathosystem teaches how to breed for durable resistance in plants
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Alain Palloix, Julie Quenouille, Estelle Paulhiac, Benoît Moury
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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The combination of major resistance genes with quantitative resistance factors is hypothesized as a promising breeding strategy to preserve the durability of resistant cultivar, as recently observed in three different pathosystems. Using the pepper (Capsicum annuum)/Potato virus Y (PVY, genus Potyvirus) pathosystem, we aimed at identifying plant genetic factors directly affecting the frequency of virus adaptation to the major resistance gene pvr23 and at comparing them with genetic factors affecting quantitative resistance. The resistance breakdown frequency was a highly heritable trait (h2=0.87). Four loci including additive quantitative trait loci (QTLs) and epistatic interactions explained together 70% of the variance of pvr23 breakdown frequency. Three of the four QTLs controlling pvr23 breakdown frequency were also involved in quantitative resistance, strongly suggesting that QTLs controlling quantitative resistance have a pleiotropic effect on the durability ofthe major resistance gene. With the first mapping of QTLs directly affecting resistance durability, this study provides a rationale for sustainable resistance breeding. Surprisingly, a genetic trade-off was observed between the durability of PVY resistance controlled by pvr23 and the spectrum of the resistance against different potyviruses. This trade-off seemed to have been resolved by the accumulation of minor-effect durability QTLs under field selection
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https://hal.inrae.fr/hal-02747945/file/Palloix%202013%20Eucarpia%20323-331_1.pdf BibTex
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Multivariate QTL analyses and predictions of yield related traits in pepper
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Nurudeen Adeniyi Alimi, M.C.A.M. Blink, Alain Palloix, F.A. van Eeuwijk
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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Yield is a key trait in pepper and is often measured simultaneously with other traits over several environments. The understanding and genetic improvement of yield may benefit from the joint analysis of yield with its related traits simultaneously. Linear mixed models have emerged as a flexible approach that correctly model underlying variance-covariance structures among the traits and between environments simultaneously. ln this study, we applied four different QTL approaches based on linear mixed model on five yield related pepper traits measured across four environments. We evaluated the performance of the approaches in terms of the number of QTLs detected for each trait and their explained variance. The QTL models are a single-trait single-environment approach (STSE), a multi-trait approach (MT), a multi-environment approach (ME), and a multi-trait multienvironment approach (MTME). We further compared prediction accuracies between STSE and MT models. The predictions were subjected to a five-fold cross validation. Our results showed that multi-trait and/or multi-environment QTL analyses are more powerful and effective to map pleiotropic QTL and QTL by environment interactions th an performing STSE analysis. The multivariate models further showed improvement over STSE in terms of both number of QTLs and the explained variance. MTME clearly outperformed ail the other methods. With MTME, nine QTLs explaining 51% of genetic variation were identified for yield in the autumn trial in Spain as against three, three and six QTLs explaining 37%,29% and 43% from STSE, ME and MT analyses, respectively. The MT model for yield in SP2 had prediction accuracy of 0.53, against 0.42 from the STSE model. These results confirmed that multivariate analyses of traits have better capabilities to unravel complex traits than single trait approach. Our result showed that trait's prediction accuracy depends not only on prediction model of choice and traits genetic architecture but also on the environmen
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https://hal.inrae.fr/hal-02748944/file/Palloix%202013%20Eucarpia%20273-280_1.pdf BibTex
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Genetic structure of the INRA Capsicum spp. collection using SSR loci: refining the wild origin of cultivated C. annuum and impact of human selection on the structuration of genetic diversity in cultivar types
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Anne-Marie Sage-Palloix, Maryse Nicolaï, Melissa Cantet, Véronique Lefebvre, Alain Palloix
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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Germplasm collections of cultivated plants constitute the source for further genetic progress and gained interest with approaches for tracking allelic variants associated to phenotypic variations within core collections. ln order to explore the structure of genetic variation in pepper (Capsicum spp.) and to select core-collections maximizing the genetic and the phenotypic diversity, a pepper collection including 1352 non redundant accessions from Il Capsicum species from 89 different countries was genotyped using 28 microsatellite (SSR) markers spanning the whole genome. Model-based analysis structured the collection into 6 clusters, with 3 clusters separating the main species complexes, including cultivated species and wild relatives, according to botanic classification (C. frutescens/C. chinense, C. baccatum, C. pubescens), and 3 additional clusters for C. annuum. The relationships between the cultivated C. annuum species and the wild relative (C. annnuum var glabriusculum) was refined. The 3 C. annuum clusters were significantly distinct for plant and fruit descriptors corresponding to cultivar types, showing that the genetic structuration of this cultivated species was strongly impacted by the long term hurnan selection of landraces in prirnary as weil as secondary diversification centres. We settled nested core-collections of 8, 16,32, 64 and 128 C annuum accessions capturing from 37% to 90% of the genetic diversity for further sequencing efforts and establishment of high through put genotyping assays. By compiling phenotypic and genotypic data, a larger core-collection of332 accessions was established, capturing 97% of the C. annuum genetic and phenotypic diversity for further genetic association studies
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https://hal.inrae.fr/hal-02746221/file/Sage-palloix%202013%20Eucarpia%20243-251_1.pdf BibTex
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Towards the deciphering of the genetic factors involved in durability of plant major resistance genes to root knot nematodes in pepper
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Arnaud Barbary, Alain Palloix, Ariane Fazari, Nathalie Marteu, Philippe Castagnone-Sereno, Caroline Djian-Caporalino
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy
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Root-knot nematodes (RKNs), Meloidogyne spp., are extremely polyphagous plant parasites worldwide. Since the use of most chemical nematicides is being prohibited, genetic resistance is an efficient alternative way to protect crops against these pests. However, few resistance genes (Rgenes) are available and some nematode populations may become able to overcome them with time. Sustainable management of these valuable resources is thus a key point of R-gene durability. ln pepper (Capsicum annuum), Me3 is a dominant major R-gene, currently used in breeding programs, that controls M. arenaria, M. incognita and M. javanica, the three main RKNs species. It was introgressed in either a susceptible or a partially resistant (i.e., that shows reduced symptoms) genetic background in either homozygous or heterozygous allelic status. Doux Long des Landes (DLL) was used as susceptible recipient pepper line and Yolo Wonder (YW) as a partially resistant one. Challenging all these genotypes with a high inoculation pressure of an avirulent M. incognita isolate demonstrated that i) the efficiency of the R-gene in reducing the reproductive potential of RKNs is strongly affected by the plant genetic background, ii) the allelic status of the R-genes has no effect on nematode reproduction. These results highlight the primary importance of the choice of both the R-gene and the genetic background into which it is introgressed during the selection of new elite cultivars by plant breeders
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https://hal.inrae.fr/hal-02749808/file/Palloix%202013%20Eucarpia%20281-288_1.pdf BibTex
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How a species becomes polyploid? The Brassica model
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Anne-Marie Chèvre, Frederique Eber, Maryse Lodé-Taburel, Gwenn Trotoux, Virginie Huteau, Olivier Coriton, Cyril Falentin, Gilles Lassalle, Emmanuel Szadkowski, Maria Loreto Suay Llopis, Maria M. Manzanares-Dauleux, Régine Delourme, Sophie Paillard, Eric Jenczewski, Karine Alix-Jenczewski
article
7.European Plant Science Organisation (EPSO) Conference, Sep 2013, Porto Heli, Greece
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How a species becomes polyploid? The Brassica model. 7.European Plant Science Organisation (EPSO) Conference
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Effect of plant genotype on the efficacy of stimulators of plant defences in two horticultural pathosystems
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Brigitte B. Maisonneuve, Caroline Allen-Aubouard, Michel Pitrat
article
4. Breeding for inducible resistance, Aug 2013, Avignon, France
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Four stimulators of plant defences (SPD) were tested on two pathosystems: powdery mildew (PM) due to Podosphaera xanthii on melon and downy mildew (DM) due to Bremia lactucae on lettuce. More than 450 accessions of melon were screened for the stimulation of their defence reactions by a formulation with laminarin, BION® 50WG and CalFlux and more than 400 accessions of lettuce with BION, Calflux and a formulation with yeast extract (ABE IT 56). In the PM/melon pathosystem, only BION was able to induce resistance; in the majority of the accessions resistance was not activated but some accessions fully susceptible to PM in the control sprayed with water were very well protected by BION. In the DM/lettuce pathosystem, the three tested SPD could induce resistance, but again BION was the most effective. Close to half accessions of the 300 susceptible to Bl: 25 and a quarter of the 110 accessions tested with Bl: 26 were well protected against the tested strain by BION and some of these were also protected by CalFlux (13% and 14%, respectively) and few by ABE IT 56 (10% and 4%, respectively). In both pathosystems, the induced resistance seems to be strain-specific as some accessions were protected against one strain and not protected against another strain; nevertheless some promising accessions were protected against both strains
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Light and temperature of ripening tomato fruits interact to regulate ascorbate synthesis, oxidation and recycling
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Hélène Gautier, Capucine Massot, Doriane Bancel, Vincent Truffault, Rebecca Stevens
article
11. International POG Conference Reactive Oxygen and Nitrogen Species in Plants, Jul 2013, Varsovie, Poland
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https://hal.inrae.fr/hal-02744410/file/Stevens_Truffault%202013%20BioTechnologia%20174_1.pdf https://hal.inrae.fr/hal-02744410/file/Stevens_Truffault%202013%20BioTechnologia%20174_2.docx BibTex
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Use of Prunus genetic diversity for peach rootstocks
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Henri Duval
article
8. International Peach Symposium, Jun 2013, Matera, Italy
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ln the Redher classification, the most widely adopted classification of Prunus L., there are more than 200 species divided into five subgenera with three for the stone fruits: Amygdalus, Prunophora and Cerasus. The major peach rootstocks are included in the subgenera Amygdalus (peach and almond species), mainly because there are no or very few problems of incompatibility grafting. Currently, the peach seedlings that are easy to produce and productive, are often used as peach rootstock, except in the calcareous soils where peachxalmond hybrids are preferred. Prunophora species are chosen for a better adaptability to heavy soils, or for introducing resistance genes to nematodes, to fungus or bacterial soil-diseases. The grafting of Prunophora species induces lower vigor for Amygdalus species and can initiate more problems of grafting incompatibility. So, a breeding program which integrates Prunophora species will be longer in time than one using only Amygdalus species. The main selected traits in the world peach rootstock breeding programs worldwide are still nematode resistance, waterlogging, vigor control, calcareous soils, and drought. For each trait, several Prunus genotypes may fit the requested characteristics, and it is important to choose the best crossing strategy which aIlows the breeder to obtain the best performance for aIl traits in order to replace an existing rootstock
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High or low fructose? Consequences for sugar metabolism in peach fruit
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Elsa Desnoues, Yves Y. Gibon, Valentina Baldazzi, Veronique Signoret, Michel M. Génard, Bénédicte Quilot-Turion
article
8. ISHS International Peach Symposium, Jun 2013, Matera, Italy. 1 p
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https://hal.inrae.fr/hal-02740591/file/Peach_Symposium_V2_1.pdf https://hal.inrae.fr/hal-02740591/file/Peach_Symposium_V2_2.doc BibTex
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Biological features, positional cloning and validation of the Ma gene for high-Ievel and complete-spectrum resistance to root-knot-nematodes in Prunus
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Daniel Esmenjaud, Cyril van Ghelder, Joel Polidori, Samira Khallouk, Henri Duval
article
Eighth International Peach Symposium, Jun 2013, Matera, Italy
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Stone fruit crops Prunus spp. grown under Mediterranean climates are severely damaged by root-knot nematodes (RKN) Meloidogyne spp. and breeding for RKN-resistant rootstocks is a promising control alternative to nematicide ban. Resistance (R) genes that confer different spectra have been identified and mapped in plums (Ma and Rjap), peach (RMia) and almond (RMja). Thus sustainable resistance in Prunus is based on pyramiding of R genes in interspecific rootstock mate rial to take into account the risk of resistance breaking in the durable plant-nematode interaction for perennials. The Ma gene from Myrabalan plum has been shown to confer a complete-spectrum, high-Ievel and heat-stable resistance to both mitotic (M. arenaria, M. incognito, M. javanico and M. enterolobii) and meiotic (M. f/oridensis) RKN. Ma triggers a hypersensitive-like reaction (HLR) in raot apices and severe nematode attacks induce the development of subterminal lateral rootlets replacing primary terminal apices and providing an acive, resist,ance response to HLR damage (1). Sustainability of resistance conferred by Ma has been challenged in multi-year experiments applying a high and continuous nematode inoculum pressure by co-cultivation of RKN-infested susceptible tomato plants together with Prunus plants carrying Ma or, for comparison, with R tomato plants carrying the Mi-l reference gene. Galling and virulent nematode individuals have been observed in Mi-l resistant tomatoes but not in Ma-carrying Prunus plants. The positional cloning of the Ma locus in accession P.2175 has been performed using high resolution mapping developed in two successive steps totalling over 3000 segregants. The Ma locus interval has been reduced to a 32-kb cluster of three TIR-NB-LRR genes (TNLl to TNL3) including a pseudogene (TNL2) and a truncated gene (TNL3). Using A. rhizogenes transformed hairy raots and composite plants (2), the best candidate gene, TNLl, comprising the genomic sequence and the native promoter region (15.3 kb), has been validated as Ma as it conferred the same complete-spectrum and high-Ievel resistance as in the donor accession P.2175. The full-Iength cDNA (2048 aa) of Ma is the longest of ail R genes cloned tc-date. Its TNL structure is extended by a huge Cvterminal post-LRR (PL) region (1088 aa) comprising five repeated PL exons (3)
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New insights on blood-flesh determinants in peach
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Thierry Pascal, Carole Confolent, Patrick Lambert, Jean-Luc Poëssel, Bénédicte Quilot-Turion, Z. Shen
article
8. International Peach Symposium, Jun 2013, Matera, Italy
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https://hal.inrae.fr/hal-02744629/file/Pascal%202013%208.%20Int.%20peach%20symp._1.pdf BibTex
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Molecular assisted selection (MAS): a promising approach under development in peach breeding
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Patrick Lambert, Elisabeth Dirlewanger, Bénédicte Quilot-Turion, Michela Troggio, Diego Micheletti, Maria Jose Aranzana, Thierry Pascal
article
8. International Peach Symposium, International Society for Horticultural Science (ISHS). INT., Jun 2013, Matera, Italy
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Facteurs biotiques et abiotiques influençant l’efficacité des SDP
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Marie Torres, Brigitte Maisonneuve, Michel Pitrat
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Colloque Elicitra, Jun 2013, Avignon, France
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Effects of genetic resistance combinations in melon on density and genetic diversity of Aphis gossypii populations in fields
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Sophie Thomas, Flavie Vanlerberghe-Masutti, Nathalie Boissot
article
9. International Symposium on Aphids (9ISA 2013), Jun 2013, Beijing, China
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Since the deployment of genetic resistant cultivars, several cases of breakdown have occurred rising the question of durability. Quantitative resistances are considered broad-spectrum and more durable than qualitative resistances but this has been poorly tested. We studied selection pressures exerted by qualitative and quantitative plant resistances in fields in the aim to understand how local adaptation of pest populations cou Id occur in agrosystems and to develop strategies for the management of resistances. Field trials were set up in three areas with different melon resistance combinations in a homogeneous genetic background, associating a major gene (the Vat gene) and quantitative trait loci (QTLs) known to affect plant acceptance in two clones of Aphis gossypii. Effects of the Vat gene and the QTLson the density and the genetic diversity of A. gossypii field populations were analyzed. Among the 138 multilocus genotypes (MLGs) identified in 2772 aphids collected, only 11 developed colonies. The Vat gene reduced density of aphid populations on melon crops in ail areas and resulted in changes of the clonai diversity and the genetic structure of aphid populations; the clonai richness was reduced to 6 MLGs in colonies collected on Vat-plants. Conversely, the QTLs did not affect aphid density and slightly affected genetic diversity. Along with selection, competition and local extinction probably condition emergence of certain MLGs on Vat-plants. Because of their narrow-spectrum, the QTLs tested did not reduce the risk of emergence of MLGs adapted ta the Vat gene when these MLGs were associated ta the Vat gene.
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Comparison of evolutionary and swarm intelligence-based approaches in the improvement of peach fruit quality
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Abdeslam Kadrani, Mohamed Mahmoud Ould Sidi, Bénédicte Quilot-Turion, Michel M. Génard, Francoise F. Lescourret
article
International Symposium on Operational Research and Applications, May 2013, Marrakech, Morocco. 9 p
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We investigated two major families of algorithms for the multi-objective optimization: evolutionary and swarm intelligence-based optimization approaches. Non-dominated Sorting Genetic Algorithm II (NSGA-II) and multi-objective particle swarm optimization (MOPSO) algorithms are biology inspired and are population-based as use a set of solutions which evolve within the search space. These approaches employ different strategies and computational effort; therefore, a comparison of their performance is needed. This paper presents the application and performance comparison of NSGA-II and one variant of the MOPSO, namely MOPSO-CD which incorporates the crowding distance computation and the constraints handling, to design ideotypes for sustainable fruit production systems. The design of peach ideotypes that satisfy the requirement of high fruit quality and low sensitivity to brown rot in a given environment was formulated as a multi-objective problem, and both NSGA-II and MOPSO-CD are used to find the best combinations of genetic resources and cultural practices adapted to, and respectful of specific environments. Statistically significant performance measures are employed to compare the two algorithms.
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https://hal.inrae.fr/hal-02809764/file/paper_Kadrani_isorap13_1.pdf BibTex
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Intégration du contrôle génétique dans des modèles
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Bénédicte Quilot-Turion
article
Séminaire "concevoir et évaluer des idéotypes", GIS GC-HP2E, Feb 2013, Paris, France
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Intégration des stimulateurs de défense des plantes dans les stratégies de protection des cultures légumières
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Brigitte B. Maisonneuve, Michel Pitrat, Marie Torres
article
Piclèg : quand chercheurs, conseillers agricoles et producteurs de légumes renouvellent les pratiques de culture, SIA (Salon de l'agriculture), Groupement d'Intérêt Scientifique pour la Production Intégrée en Cultures légumières (Gis Piclèg). FRA., Feb 2013, Paris, France
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https://hal.inrae.fr/hal-02805886/file/Maisonneuve_Pitrat%202013%20SIA%20Paris_1.pdf BibTex
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Quantitative resistance loci reduce the breakdown frequency of a major resistance gene. A relevant way for durable resistance breeding
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Julie Quenouille, Estelle Paulhiac, Benoît Moury, Alain Palloix
article
12. IPVE Symposium, International Committee on Plant Virus Epidemiology (ISPP). St Paul.; International Institute of Tropical Agriculture, CGIAR (IITA). Ibadan, NGA., Jan 2013, Arusha, Tanzania. 256 p
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The combination of major resistance genes with quantitative resistance factors is hypothesized as a promising breeding strategy to preserve the durability of resistant cultivars. In three pathosystems, experimental data demonstrate that the durability of a major resistance gene depends on the plant genetic background but the genetic factors involved are still unknown. Using the pepper (Capsicum annuum)/Potato virus Y (PVY)) pathosystem, we aimed to identify genetic factors directly involved in pvr23 resistance breakdown frequency and to compare them with genetic factors affecting quantitative resistance. For QTL mapping experiments, 156 doubled haploid lines carrying the pvr23 resistance allele were tested for pvr23 resistance breakdown frequency, virus accumulation and symptom intensity. Four loci including additive QTLs and epistatic interactions explained together 70% of the variance of pvr23 breakdown frequency. Comparative mapping of the different traits showed that three of the four QTLs controlling the breakdown frequency of the pvr23 allele were also involved in quantitative resistance, indicating that QTLs for quantitative resistance have a pleiotropic effect on the durability of the major resistance gene. This study provides the first mapping of QTLs directly affecting resistance durability and opens the way for sustainable resistance breeding.
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Specificity of eIF4E factors towards resistance to potyviruses in Arabidopsis thaliana
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Aramata Marena, Caroline Callot, André Moretti, Jean-Luc J.-L. Gallois
article
14. Rencontres de Virologie Végétale (RVV 2013), Jan 2013, Aussois, France
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The translational initiation factors eIF4E represent the main resistance to Potyvirus as well as to other families of plant RNA virus. Although those factors are encoded by small multigenic families, it has been shown that each virus can only use specific eIF4E to perfom its cycle in plants. Often, this coincides with the ability of the virus VPg to interact with those host proteins. Using Arabidopsis transgenic approaches, we aimed at better understanding the basis of the virus specificity for eIF4E.
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https://hal.inrae.fr/hal-02745414/file/Gallois%202013%2014.%20RVV_1.pdf BibTex
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The chloroplast phosphoglycerate kinase is involved in rwm1-mediated resistance to Water melon mosaic potyvirus in Arabidopsis
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Laurence Ouibrahim, Joan Estevan, Marianne Mazier, Hervé Lecoq, Cecile Desbiez, Gaelle G. Pagny, Véronique Decroocq, André Moretti, Carole Caranta
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14. Rencontres de Virologie Végétale, Jan 2013, Aussois, France
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Genetic variability study in a wide germplasm of domesticated peach through high throughput genotyping
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Diego Micheletti, Valeria Aramini, Pere Arus, Elisa Banchi, Teresa Barreneche, Daniele Bassi, Elisabeth Dirlewanger, Zhongshan Gao, Laura Gazza, Patrick Lambert, Francois Laurens, Xiongwei Li, Thierry Pascal, Bénédicte Quilot-Turion, Laura Rossini, Michela Troggio, Ignazio Verde, Maria Jose Aranzana
article
21. Plant and Animal Genome Conference, Jan 2013, San Diego, United States
resume
Peach (Prunus persica (L.) Batsch) is one of the most economically important fruit crops in temperate areas. Classical fruit tree breeding is generally slow and inefficient. Molecular markers could improve its efficiency but, although nowadays many Mendelian traits are mapped in peach and SSR markers have been found to be linked to some of the key major genes, its use in breeding programs is still limited. Main reasons for that are insufficient linkage between the markers and the genes and the lack of markers suitable for medium-high degree of multiplexing. To address this limitation, about 1,300 peach cultivars were genotyped with the 9K peach SNP chip (Verde et al. 2012) in the frame of FruitBreedomics project. This germplasm was chosen to be representative of the genetic diversity present in five germplasm collection in Europe and in China. Out of the 8144SNPs present in the chip, about 4300 were positively genotyped and used for the further analysis. The average number of heterozygous loci in the genotyped accessions was 1186 (spanning from 13 to 2775). The preliminary results of the population structure reveal three main subpopulations and the presence of high number of admixed individuals. LD seems to decay at distance longer than ca. 1 Mb. These results will be instrumental for implementing LD-based mapping of QTLs and genes in peach.
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Poster communications

titre
Variation for fruit shape morphology and candidate genes in eggplant materials
auteur
M. Hurtado, M. Plazas, P. Gramazio, S. Vilanova, Marie-Christine Daunay, E. van Der Knaap, J. Prohens
article
15. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2013, Torino, Italy. 2013, Breakthroughs in the geneties and breeding of capsicum and eggplant
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https://hal.inrae.fr/hal-02748085/file/Daunay%202013%20Eucarpia%20381_1.pdf BibTex
titre
A MAGIC population to decipher the genetic control of quantitative traits in tomato
auteur
Laura Pascual, Mathilde M. Causse, Jiaxin Xu, Christopher Sauvage, Guillaume Bauchet, Nelly Desplat, Jean-Paul Bouchet, Aurore Desgroux, Dominique D. Brunel, Marie-Christine Le Paslier
article
7. EPSO Conference, Sep 2013, Porto Heli, Greece. 2013
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https://hal.inrae.fr/hal-02748316/file/Pascual_Causse_Sauvage%202013%20Poster_1.pdf BibTex
titre
The cell wall of tobacco and tomato pollen tubes contains fucosylated xyloglucan not found in somatic cells
auteur
Flavien Dardelle, Arnaud Lehner, Muriel Bardor, Christophe Rihouey, Mathilde Causse, Patrice Lerouge, Azeddine Driouich, Jean-Claude Mollet
article
XIIIth Cell Wall Meeting, Jul 2013, Nantes, France
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titre
How gene flow between wild and cultivated lettuce can influence the dynamic of adaptation of Bremia lactucae
auteur
Romain R. Valade, Brigitte B. Maisonneuve, Aurore Ducasse, R. Michelmore, Claire Neema
article
1. International Conference Wild Plant Pathosystems, Jul 2013, Olomouc, 2013
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https://hal.inrae.fr/hal-02746835/file/Valade_Maisonneuve%202013%20Wild%20Plant%20Pathosystems%20poster_1.pdf BibTex
titre
Building high-density peach linkage maps based on the ISPC 9K SNP chip for mapping mendelian traits and QTLs: benefits and drawbacks
auteur
Jehan-Baptiste Mauroux, Bénédicte Quilot-Turion, Thierry Pascal, Elisabeth Dirlewanger, José Antonio Campoy-Corbalan, M. Troggio, I. Verde, D. Micheletti, M.J. Aranzana, Patrick Lambert
article
8. ISHS International Peach Symposium, Jun 2013, Matera, Italy. 1 p., 2013
resume
“FruitBreedomics” European project has been designed by an international consortium including scientists, stakeholders and breeding companies. One of its aims is to increase the efficiency of breeding programs in apple and peach by developing Marker Assisted Breeding (MAB) using novel genomic tools. In the frame of this project, six peach mapping populations (two intraspecific F2, two interspecific F1 derived from P. davidiana, one self pollinated derived from Bolinha and one advanced pseudo-backcross) segregating for mendelian traits and/or Quantitative Trait Loci (QTLs) for pest resistance and fruit quality were genotyped using an Infinium II Illumina bead-chip containing 8144 SNPs derived from the sequencing of the peach genome. Mapping results were contrasted depending on the population considered. Relevancy of using general medium-range SNP bead- chips for breeding purposes is discussed in this study
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https://hal.inrae.fr/hal-02808695/file/poster_matera_JB_v5_PL_final_1.pdf BibTex
titre
Combination of genetic resistance and management of field borders: towards an effective and sustainable control of Aphis gossypii outbreaks and virus epidemics in cantaloupe melon crops
auteur
Alexandra Schoeny, Patrick Gognalons, Nathalie Boissot, Pascale Mistral, Virginie Chareyron, Catherine Wipf-Scheibel, Hervé Lecoq
article
9. International Symposium on Aphids (9ISA 2013), Jun 2013, Pékin, China. 2013, 9ISA Program & Abstracts book
resume
Aphis gossypii is a major pest of cantaloupe melon crops, causing direct damage (sap uptake) and indirect damage (virus transmission). The cantaloupe gene Vat confers resistance to colonization by A. gossypii and resistance to the non-persistent viruses (CMV, WMV, etc) carried by A. gossypii. lt is however ineffective in blocking the transmission of viruses carried by other aphid species and its use is thus generally coupled with aphicide treatments to limit virus transmission by non-colonizing aphids. Moreover, due to its intraspecific variability, A. gossypii can adapt to various selective pressures (host plants, insecticides); this adaptation ability enhances the risk of resistance breakdown. ln France, cantaloupe protection faces a double challenge: to reinforce the effectiveness of Vat in a context of reducing pesticide use, and ensure the sustainability of Vat in a context of increasing Vat deployment. The hypothesis tested in this project is that an appropriate management of field borders can contribute to regulating the populations of aphids and/or their virus load, thereby reducing the risks of virus epidemies and resistance breakdown. lndeed, literature suggests that flower strips can participate in pest biological control by favoring natural enemies (predators and parasitoids), and strips of non host plants can protect crops from non-persistent viruses by allowing aphids to probe on healthy plants and thus to lose their virus load before reaching the crops. An experimental field approach was initiated to compare three types of field border management (bare soil, grass strip and flower strip) on the effectiveness and sustainability of Vat.
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https://hal.inrae.fr/hal-02749673/file/2013%20ISA%20Beijing%20abstract%20Schoeny_1 BibTex
titre
Diversification of host targets to promote resistance against Potyvirus and Begomovirus in tomato
auteur
Camille Gauffier, Caroline Lebaron, André Moretti, C. Constant, F. Moquet, G. Bonnet, Carole Caranta, Jean-Luc J.-L. Gallois
article
14. Rencontres de Virologie Végétale (RVV 2013), Jan 2013, Aussois, France. 2013
resume
Tomato (Solanum lycopersicum) is one of the most cultivated vegetable in the world, but suffers from important yield losses caused by viral diseases. Therefore, the development and use of cultivars that are genetically resistant to viruses has become a critical factor of competitiveness for both breeders and producers and one of the key stakes for sustainable agriculture. In this context, generating new resistance alleles using biotechnological approaches (e.g., TILLING) appears as a powerful tool to diversify host targets to promote resistance against viruses.
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Poster communications
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https://hal.inrae.fr/hal-02745583/file/Gauffier_Gallois%202013%2014.%20RVV%20Poster_1.pdf BibTex
titre
Couplage entre résistance génétique et aménagement parcellaire : vers une gestion efficace et durable du puceron Aphis gossypii et des épidémies virales en cultures de melon
auteur
Alexandra Schoeny, Patrick Gognalons, Nathalie Boissot, Pascale Mistral, Virginie Chareyron, Catherine Wipf-Scheibel, Hervé Lecoq
article
14. Rencontres de Virologie Végétale (RVV 2013), Jan 2013, Aussois, France. 2013
resume
Le gène Vat confère au melon une résistance à la colonisation par le puceron Aphis gossypii ainsi qu'une résistance aux phytovirus non persistants (CMV, WMV,...) transmis par ces pucerons (Lecoq et al., 1979; Pitrat & Lecoq, 1982 ; Boissot et al., 2010). Il est toutefois inefficace pour bloquer la transmission des virus portés par les autres espèces de pucerons. L'utilisation de Vat est donc généralement couplée à des traitements aphicides afin de limiter la transmission virale par les pucerons non colonisateurs du melon. Or, la réduction progressive de l'usage des produits phytosanitaires dans la protection des cultures imposée par l'évolution de la réglementation (plan Eco-Phyto 2018) conduit à rechercher de nouvelles stratégies permettant d'accompagner la lutte génétique pour la gestion des bio-agresseurs. La bibliographie suggère que l'implantation de bandes de plantes non hôtes (enherbées ou fleuries) à la périphérie des cultures pourrait être une option efficace pour diminuer 1) la pression puceron (et donc le risque de contournement de Vat) en favorisant le développement des ennemis naturels (Pfiffner & Wyss, 2004) et 2) la pression virus en constituant notamment un filtre réduisant la charge virale des pucerons avant qu'ils n'atteignent les cultures (Hooks & Fereres, 2006). L'hypothèse testée dans ce projet est qu'un aménagement adéquat de l'environnement parcellaire peut contribuer à réguler les populations de pucerons et/ou leur potentiel virulifère et ainsi à réduire le risque d'épidémies virales. L'effet de trois types d'aménagement (sol nu, bandes enherbées et bandes fleuries) sur l'efficacité de la résistance médiée par le gène Vat est évalué au champ.
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https://hal.inrae.fr/hal-02749408/file/Schoeny_1 BibTex
titre
Exploring genetic diversity in tomato fruit with a systems biology approach
auteur
Laura Pascual, Jiaxin Xu, Nelly Desplat, Benoit B. Valot, Jean-Paul Bouchet, Dominique D. Brunel, Mathilde M. Causse
article
21. International Plant and Animal Genome, Jan 2013, San Diego, United States. 2013
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https://hal.inrae.fr/hal-02745690/file/Pascual_Causse_Bouchet%202013%20Poster_1.pdf BibTex

Reports

titre
Adaptation de l’abricotier aux changements climatiques : estimation des risques génétiques et géographiques liés aux avortements floraux et à l’époque de floraison Le réseau agroclimatique de l’INRA et la base associée de données
auteur
Inaki Garcia de Cortazar Atauri, Patrick Bertuzzi, Lucia Andreini, François Madon, Jean Marc Audergon
article
[Contrat] 2013
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https://hal.inrae.fr/hal-02809978/file/Raport%20final%20CTPS%20Abricot%202013_1.pdf BibTex

2012

Conference papers

titre
QTL analyses on genotype-specific component traits in a crop simulation model for Capsicum annuum L.
auteur
A. M. Wubs, E. Heuvelink, J. A. Dieleman, J. J. Magan, Alain Palloix, F. A. van Eeuwijk
article
4th International Symposium on Models for Plant Growth, International Society for Horticultural Science (ISHS). INT., Nov 2012, Nanjing, China
resume
QTL for a complex trait like yield tend to be unstable across environments and show QTL by environment interaction. Direct improvement of complex traits by selecting on QTL is therefore difficult. For improvement of complex traits, crop growth models can be useful, as such models can dissect a target trait into a number of component traits. QTL for the component traits are assumed to be more stable across environments. The target trait can be reconstructed from its component traits together with environmental inputs. Instead of observed component traits, QTL fits for component traits may be used when QTL explain a reasonable proportion of the variation in the components. We applied this dissection approach to the target trait total shoot biomass for a population of 149 recombinant inbred lines from the intraspecific cross of Capsicum annuum ‘Yolo Wonder’ and ‘Criollo de Morelos 334’. A simple LINTUL-type simulation model was used, with rate of change of leaf area index and light use efficiency as genotype specific component traits. These two component traits were determined in four phenotyping experiments (spring and autumn cultivations in the Netherlands and Spain), and subjected to QTL analysis. Seven QTL were found for both component traits. For leaf area index development rate 40 to 50% of the observed variance was explained by the QTL, while this was slightly lower for light use efficiency (23-39%). Using the QTL fitted values of the component traits following QTL analysis, the crop simulation model explained 27- 43% of the observed variation in total shoot biomass, which was higher than the variation explained by the QTL for total shoot biomass itself for most experiments. The approach of dissecting a complex trait into its component traits is therefore a promising one. Next step is to extend the model with biomass partitioning
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https://hal.inrae.fr/hal-02747071/file/qtlanalyses_2012_1.pdf BibTex
titre
Fruit size in relation to competition for resources: A common model shared by two species and several genotypes grown under contrasted carbohydrate levels
auteur
Marion Prudent, Zhanwu Z. Dai, Philippe Vivin, Michel M. Génard, Nadia Bertin, Mathilde M. Causse
article
4. International Symposium on Plant Growth Modeling, Simulation, Visualization and Applications, IEEE., Oct 2012, Shanghai, China
resume
Fruit size is one important criterion of fruit external quality affecting consumer acceptance. The effects of seed number on fruit size in two fleshy fruits, grape and tomato, of different genotypes and grown under distinct carbohydrate availability levels were analyzed with a model. The two-parameter model described within-fruit resource competition and was able to well represent the commonly observed decrease in fresh weight per seed along with the increase in number of seeds, regardless of species, genotypes, and carbohydrate levels that were evaluated in this study. However, carbohydrate levels largely modified the correlation between seed number and fresh weight per fruit. In grape, lowering carbohydrate level led to a decrease in the parameter reflecting the potential fresh weight per seed, while in tomato, the response to carbohydrate was genotype-dependent and concerned more the parameter reflecting the competition level for resources. The values of the latter parameter suggested an under-compensating competition for resources in domesticated grape and tomato genotypes. Finally, co-localizations among quantitative trait loci for fruit fresh weight and model parameters indicated that plant susceptibility to competition could underlie the fruit fresh weight determination.
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titre
Compromising NSGA-II performances and stopping criteria: case of virtual peach design
auteur
Mohamed Ould Sidi, Abdeslam Kadrani, Bénédicte Quilot-Turion, Francoise F. Lescourret, Michel M. Génard
article
4. International Conference on Metaheuristics and Nature Inspired Computing, META'2012, Oct 2012, Port El-Kantaoiui, Tunisia
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titre
Protection des laitues contre le mildiou. Diversité génétique de la réactivité aux SDP
auteur
Brigitte Maisonneuve, Caroline Allen-Aubouard, Michel Pitrat
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2012, Avignon, France
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https://hal.inrae.fr/hal-02811361/file/Maisonneuve-Pitrat%202012Miffel%20Avignon_1.pdf BibTex
titre
Intégration du côntrole génétique dans les modèles
auteur
Bénédicte Quilot-Turion
article
Ecole-chercheurs "Conception d'idéotypes de plante pour l'agriculture durable", Oct 2012, Seillac, France
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titre
Experimental evidence of the efficiency of two resistance genes deployment strategies - pyramiding or alternating - for sustainable management of root-knot nematodes
auteur
Caroline Djian-Caporalino, Ariane Fazari, Nathalie Marteu, Anne-Marie Sage-Palloix, S. Risso, R. Lanza, Alain Palloix, Philippe Castagnone-Sereno
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France
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https://hal.inrae.fr/hal-02744512/file/Sage-Palloix_Palloix%202010%20Plant%20resistance%20sustainability%2042_1.pdf BibTex
titre
Contribution of microbial core-collections and exploitation of polygenic resistances to the development of Phytophthora control strategies in solanaceous crops
auteur
Marie-Line Kuhn, Anne Massire, Melissa Cantet, Rene Damidaux, Elvina Gibowski, Youna Ruellan, Véronique Lefebvre, Franck Panabières
article
Plant Resistance Sustainability 2012 International Conference, Oct 2012, La Colle sur Loup, France
resume
Oomycetes form a diverse group of fungus-like eukaryotic microorganisms that include saprophytes as weil as pathogens of animais and some of the most devastating pathogens of dicotyledonous plants. Among them, few species of the genus Phytophthora, such as P. capsici and P. infestons cause enormous economic da mage on Solanaceous crops. Major genes have been identified in tomato to confer resistance against P. infestons, but were rapidly overcome by virulent populations: Pepper resistance towards P. capsici is provided by several quantitative trait loci (QTls). Efficient exploitatio-n of such polygenic resistance sources in plant breeding programs requires a good evaluation of their spectrum and durability. This requires a precise knowledge of the diversity of pathogen populations (with a focus on virulence, aggressiveness and host range) and some of their adaptive traits, such as changes in virulence or adaptation to prevalent cultivars. Theoretically, the use of Phytophthora corecollections reflecting the overall intraspecific diversity would help to assess the a priori durability of resistance sources. We initiated a study to evaluate the potential sustainability of polygenic pepper and tomato resistances to Phytophthora. It implied i) to estimate the diversity of P. infestans and P. caps ici collections and their evolution over time; ii) to develop diverse quantitative and qualitative pathogenic assays to identify the various components underlying polygenic resistances. Collections included isolates from various geographic origins. Genetic diversity was investigated using a combination of neutral markers and genes under positive selection. First results on P. capsici reveal significant variations in the structure and dynamics of regional populations, and suggest that P. caps ici core-collections may be of local interest. We also showed that P. infestons populations are diverse but structured. Hence, strains isolated on tomato or potato differ at the genotypic and phenotypic levels. The use of P. infestons core-collections in breeding programs therefore requires gaining knowledge on the structure and epidemiological characteristics of natural pathogen populations collected on tomato.
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https://hal.inrae.fr/hal-02749550/file/Massire_Damidaux%202012%20Plant%20resistance%20sustainability%2072_1.pdf BibTex
titre
New insights on virulence and evolutionary dynamics of the Ralstonia solanacearum species complex -keys and challenges in the search of durable resistance in solanaceae
auteur
Emmanuel Wicker, Aurore Lebeau, Flora Pensec, Marie-Christine Daunay, Jacques Dintinger
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France
resume
Bacterial wilt, caused by the soilborne betaproteobacterium Ralstonia solanacearum, is a major disease of solanaceous crops, throughout tropical and subtropical areas, and emerging in temperate regions. If genetic resistance is still considered as the most promising control measure, breeding for resistance to Ralstonia solanacearum (Rsol}, has been hindered for decades by the scarcity of high level resistance sources, strong genotype x environment interactions, and the huge genomic and phenotypic plasticity of the pathogen. Ralstonia solanacearum is a species complex composed of four phylotypes related to geographical origins (1: Africa-Asia, Il: American, III: Africa, IV: Indonesia). Solanaceae-pathogenic strains belong to ail four phylotypes, but most epidemiologically active strains belong to phylotype l, liA, IIB, and III, phylotype 1being the most widespread lineage. Extensive screenings of resistant accessions of tomate, eggplant and pepper towards a worldwide R.sol core-collection, lead to formalize grain Solanaceae-Rsol interactions into six main virulence profiles, and allowed to identify highly resistant accessions, mainly in eggplant and pepper. Among the rn, the eggplant MM960 was demonstrated to carry a phylotype I-resistance major dominant gene (Ersl) and two partial resistance QTLs with strain-specific expression. We identified patterns of strain -specificity, but found no evidence of phvlotvpe-specificltv.'phvlotvpe 1containing four of the six pathopprofiles. Searching for durable resistance implies both the identification of bacterial genes responsible for avirulence and virulence, and assessment of the pathogen evolutionary potential. Considering "immune" accessions (five eggplants, two peppers, one tomato) giving true incompatible interactions (no wilt and no colonization), we then searched for genetic factors associated with bacterial virulence and avirulence using comparative genomics hybridization (CGH) data. We thus identified 61 type III effectors fairly to highly associated to avirulence to eggplants Dingras, SM6, Ceylan, Surya, and MM960, peppers CA8 and Perennial, and tomate HW7996. Interestingly, some of these effectors were elsewhere shown to contribute to bacterial fitness. Focusing on the accessions MM960 and Dingras, less than 10 genes were highly associated with avirulence, among which popP2, avirulence gene interacting with the RRS1-Rresistance gene in Arabidopsis thaliana. We inferred evolutionary dynamics of the R.solanacearum species complex (RSSC) at a global scale, performing multi-Iocus sequence analysis (MLSA) and gene genealogies on a worldwide collection. Although recombination was found ubiquitous within the RSSC, we observed phylotype-specific recombination rates and demographic histories. Phylotype 1 (and, to a lesser extent, phylotype III) is highly recombinogenic, and carries molecular signatures of a recent and rapid demographic expansion. Phylotype Il is actually composed of two evolutionary lineages, one (liA) being moderately recombinogenic and expanding, and the other (IIB) being clonai and poorly expanding. Taken together, these findings strongly suggest that phylotype 1 is the most prone to adapt rapidly to new hosts and varieties, thank to its recombination and mutation rates, capacity to disseminate, and large virulence range. To decipher evolutionary dynamics leading to adaptation at the local, field scale, an experimental evolution trial is in course to monitor the impact of Ersl-carrying eggplant MM960 successive craps on the population structure and putative avirulence genes evolution.
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https://hal.inrae.fr/hal-02745024/file/Daunay%202012%20Plant%20resistance%20sustainability%2068-69_1.pdf BibTex
titre
Effects of lettuce cultivar mixtures, differing for resistance genes, on Bremia lactucae incidence
auteur
Brigitte Maisonneuve, Eric Martin, Loic Jean, Claude C. Pope de Vallavieille, Michel Pitrat
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France
resume
Bremia lactucae, the causal agent of lettucedowny mildew,is still an important problem in lettuce production in spite of many resistance genes used over the last 50 years in Europe. Every new gene introduced into commercial varieties has been overcome in a few years. At INRA, new resistance genes were found in two L. virosa accessions and introgressed into butterhead lettuce (lines named Vi). These resistance genes are not yet introduced in commercial varieties; we would like propose a using strategy of these genes that could increase their durability. Which strategies might be efficient to reduce the evolution of the pathogen and ensure a durable resistance with these genes? A lower selection pressure on B. factucae population could reduce the speed of breakdown of the new resistance. A possibility to have a lower selection pressure on the B. factucae populations could be mixture of two cultivars differing for resistance genes. Therefore Bremia contamination has been studied for several winters in cultures of these resistant Vi lines mixed with a susceptible cultivar. ln the same culture, the progression of Bremia on the susceptible plants has been observed. The disease development has been studied after an artificial contamination with two objectives: (i) does the pathogen overcome the resistance, (ii) is the disease seve rit y on the susceptible cultivar acceptable for commercial use? After eight experimentations under a polythene greenhouse, pure stands were compared to mixture in ratio 1:3 (susceptible:resistant) after focal inoculation with a B. factucae isolate common in the region. No sporulation has been observed on the two Vi lines issued from L. virosa. For the vear with high inoculum pressure, the cultivar mixture did not reduce the disease seve rit y on the susceptible cultivar (for example, in winter 2006-2007, 24 contaminated leaves per susceptible plant in pure stand and in mixture; a disease seve rit y of 53 and 48 on the susceptible variety, respectively in pure stand and in mixture). For intermediate to low inoculum pressure, the disease development was slower on the susceptible cultivar grown in mixtures, with less contaminated plants and less contaminated leaves per plant (1.1 in pure stand vs 0.2 in mixture, in winter 2005-2006) at harvest. The disease severity on susceptible cultivar is lower in mixture than in pure stand for example in winter 2005-2006 (index 1.3 vs 9.5); this seve rit y was similar at the beginning of the epidemics for both treatments, but it became much higher in pure susceptible stand on the last week before harvest. Therefore the mixed culture could be an interesting method to reduce disease level on a cultivar susceptible to the strain that is important in the culture and also possibly increase the durability of new resistance by a lower selection pressure on the virulent strains. Nevertheless this cultural practice must be adapted to the needs of growers economically and technically; it could be difficult to have an efficient design of the mixture in a commercial culture. Some other experiments must be done in connection with the growers and the breeders.
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titre
Effects of resistance combinations in melon on Aphis gossypii field populations
auteur
Sophie Thomas, Flavie Vanlerberghe-Masutti, Virginie Chareyron, Pascale Mistral, Nathalie Boissot
article
Congres international plant resistance sustainability 2012, Oct 2012, La Colle-Sur-Loup, France
resume
Host-plant resistance has proven its efficiency in controlling pests, including aphids which are one of the major hemipteran pests in crops. Nevertheless, resistance-breaking biotypes have occurred in several plant-aphid systems. The aim of our field study was to investigate the impact of distinct combinations of aphid-resistance Vat gene and QTLs in melons on the density and genetic structure of field populations of the melon aphid species Aphis qossvpii. We set up field trials with melons combining (i) resistance alleles both at the Vat locus and at QTL (Vat/QTL), (ii) resistance allele at the Vat locus and susceptible alleles at QTL (Vat/-) or (iii) susceptible allele at the Vat locus and resistant alleles at QTL (-/QTL). Ali combinations had a homogenous genetic composition except at the Vat locus and QTL (Boissot et al 2010). Twelve trials were conducted in three distant melon producing areas,.: Southeastern France (SE), southwestern France (SW) and French West Indies (FWI), to take into account that aphid populations are geographically structured (Thomas et al 2012). Aphid population densities were significantly lower on Vat/QTL melon plants than on -/QTL plants over trials, revealing a strong effect of the Vat gene in situ. Conversely, no effect of the QTL was observed on aphid density. We also collected about 2800 aphids from these trials that were distributed in two classes whether they were found as isolated apterous individuals on the leaf soon after melon plantation or whether they were collected from a colony later on. Each aphid was genotyped for eight microsatellite loci. Whatever the resistance combination and area, the genetic diversity decreased dramatically during the melon development, from isolated apterous to colonies, which illustrated a strong host plant selective pressure. We also noticed a Vat gene effect in reducing the genetic diversity of both apterous populations in SE and colonies in SE and SW. Furthermore, according to a genetic clustering analysis, the 138 multilocus genotypes identified were separated in two clusters calied the NMl cluster and the Cucurbit luster. Aphids from the NMl cluster were significantly more affected by the Vat gene as apterous individuals were less numerous on Vat/QTL than on -/QTL melons and they did not develop into colonies on Vat/QTL and on Vat/- melons. ln conclusion, this field study did not provide experimental evidence that the combination of qualitative effective major gene and quantitative polygenic resistance to A. qossypii in melon improves control of the aphid populations. QTLs had no effect on demography or on clonai diversity and structure of aphid populations. On the contrary, the effect of the major resistance gene Vat was shown to de pend on the clonai diversity and structure ofthe aphid populations. However, this clonai diversity might substantially vary in time and space according to many ecological factors, which could prevent or at least slow down the spread of a resistance breaking biotype.
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The GEDUNEM project: Varietal and technical innovations for the sustainable and integrated management of root-knot nematodes in protected vegetable cropping systems
auteur
Caroline Djian-Caporalino, Alain Palloix, Mireille Navarrete, Amélie Lefevre, Thierry Mateille, Hélène Védie, Claire Goillon, Yannie Trottin, P. Boniol, Philippe Castagnone-Sereno
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France
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The recent banning of the most active chemical nematicides raised root-knot nematodes (RKN) as the major problem of vegetable growing in the south-east of France, with over 40% of horticultural farms affected. Resistant (R) cultivars successfully limit nematode attacks of the crop itself and reduce nematode abundance in soil in the short term. But their use in commercial fields faces two major constraints: (1) the limited number of cultivated species with RKN R-genes available (tomate. pepper), which would lead to major changes in crop rotations with socio-economic constraints; (2) the emergence of virulent root-knot nematode populations, able to overcome the resistance conferred by some of the R-genes. Recent research showed that the durability of the resistance per se was possibly increased with an optimal choice of the R-gene or allele combination, and of the genetic background in which the major R-genes where introgressed. However, the requirement for agronomic performance, does not always allow the breeder to fully use the genetic diversity available in R-genes and genetic backgrounds. ln such cases, spatio-temporal deployment strategies of resistant cultivars in the rotation were proposed and provided some success. But combination of genetic resistance with cultivation practices including multicrop rotations, intercultural management and/or prophylactic treatments was poorly tested for its ability to provide complementary selection pressures on the pathogen populations and to increase the durability of the protection. The project aims at identifying innovative strategies across the agrosystem, cornbining varietal resistance in crop rotations with agronomic practices, for a sustainable control of RKN that infect vegetable crops in protected crop systems. This pilot and demonstrative project involves analytical and systemic aspects: il the validation of previous results on the durability of RKN genetic resistance in tomato and pepper by long-term experiments in research stations and in farms, ii/ the impact of agronomic practices on the parasitic pressure in the soil and its contribution in the increase of resistance durability, and iii/ the impact of such innovations on the yield and the economie viability for protected crop systems in a Mediterranean climate. The added value here is to combine together currently dispersed approaches based on synergistic and long-term effects, towards a satisfying level of nematode control over pluri-annual crop sequences. The diversity of partners (research, experimental stations, technical institutes) and associated forces (growers' development structures, 'chambres d'agriculture') brings ail the complementary expertise needed for answering specifie short-term questions as weil as generic mid-long term expectations. 'GEDUNEM' has been launched in the framework of the INRA metaprogramme SMaCH (Sustainable Management of Crop Health).
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https://hal.inrae.fr/hal-02750319/file/Palloix%202012%20Plant%20resistance%20sustainability%2084-85_1.pdf BibTex
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Biological features, positional cloning and validation of the Ma gene for high-Ievel and complete-spectrum resistance to root-knot-nematodes in Prunus
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Cyril van Ghelder, Samira Khallouk, Joel Polidori, Henri Duval, Marc M. Kleinhentz, Elisabeth Dirlewanger, Daniel Esmenjaud
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France
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Stone fruit crops Prunus spp. grown under Mediterranean climates are severely damaged by root-knot nematodes (RKN) Me/oidogyne spp. and breeding for RKN-resistant rootstocks is a promising control alternative to nematicide ban. Resistance (R) genes that confer different spectra have been identified and mapped in plums (Ma and Rjap), peach (RMia) and almond (RMja). Thus sustainable resistance in Prunus is based on pyramiding of R genes in interspecific rootstock mate rial to take into account the risk of resistance breaking in the durable plant-nematode interaction for perennials. The Ma gene from Myrabalan plum has been shown to confer a complete-spectrum, high-Ievel and heat-stable resistance to both mitotic (M. arenaria, M. incognito, M. javanico and M. entera/obii) and meiotic (M. f/oridensis) RKN. Ma triggers a hypersensitive-like reaction (HLR) in raot apices and severe nematode attacks induce _ the development of subterminal lateral rootlets replacing primary terminal apices and providing an acive, resist,ance response to HLR damage (1). Sustainability of resistance conferred by Ma has been challenged in multi-year experiments applying a high and continuous nematode inoculum pressure by co-cultivation of RKN-infested susceptible tomato plants together with Prunus plants carrying Ma or, for comparison, with R tomato plants carrying the Mi-l reference gene. Galling and virulent nematode individuals have been observed in Mi-l resistant tomatoes but not in Ma-carrying Prunus plants. The positional cloning of the Ma locus in accession P.2175 has been performed using high resolution mapping developed in two successive steps totalling over 3000 segregants. The Ma locus interval has been reduced to a 32-kb cluster of three TIR-NB-LRR genes (TNLl to TNL3) including a pseudogene (TNL2) and a truncated gene (TNL3). Using A. rhizogenes transformed hairy raots and composite plants (2), the best candidate gene, TNLl, comprising the genomic sequence and the native promoter region (15.3 kb), has been validated as Ma as it conferred the same complete-spectrum and high-Ievel resistance as in the donor accession P.2175. The full-Iength cDNA (2048 aa) of Ma is the longest of ail R genes cloned tc-date. Its TNL structure is extended by a huge Cvterminal post-LRR (PL) region (1088 aa) comprising five repeated PL exons (3).
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https://hal.inrae.fr/hal-02745737/file/Duval%202012%20Plant%20resistance%20sustainability%2093_1.pdf BibTex
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Constraints on resistance and pathogenicity factors and their consequences on resistance durability
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Benoît Moury, Carole Caranta, Sophie Ewert, Séverine Lacombe, Frederic Fabre, Alain Palloix
article
Plant resistance sustainability 2012 international conference, Oct 2012, La Colle-Sur-Loup, France
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The durability of a major resistance gene is affected by quantitative trait loci which also confer quantitative resistance to virus
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Julie Quenouille-Lederer, Estelle Paulhiac, Pascale Mistral, Ghislaine Nemouchi, Anne-Marie Sage-Palloix, Bruno Savio, Vincent Simon, Benoît Moury, Alain Palloix
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Plant resistance sustainability 2012. International conference, Oct 2012, La Colle-Sur-Loup, France
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https://hal.inrae.fr/hal-02744632/file/2012-Quenouille-La%20colle%20sur%20loup_1.pdf BibTex
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Characterization of adaptation to a resistance gene with a pleiotropic effect, the Vat gene in melon
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Nathalie Boissot, S. Thomas, Virginie Chareyron, Pascale Mistral
article
Congrès international plant Resistance sustainability 2012, Oct 2012, La Colle-Sur-Loup, France. 2 p
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The deployment of resistance genes against aphids, is restricted because few genes have been characterised. The Vat gene, conferring resistance to Aphis gossypii, has been effectively deployed in melon crops since 20 years in South-eastern France. We have studied the adaptation of A. gossypii populations to that Vat gene. Seven clones of A. gossypii were collected in melon producing areas where the Vat gene has been or not deployed. Theses clones were characterized using 8 SSR markers. The phenotypic variability of A. gossypii was shown matching the genetic variability revealed by eight microsatellite markers (Thomas et al, 2012). We phenotyped the seven clones using three resistant melon lines. One line was susceptible to ail clones, the two other lines, Margot and 90625, were known to carry different alleles at the Vat locus. We took advantage of the double phenotype mediated by the Vat gene, the resistance to A. gossypii and the resistance to viruses (CMV, ZYMV...) when inoculated by A. gossypii - melon plants are susceptible to viruses when they are mechanically inoculated or inoculated by other aphids species. Aphid clones triggered three phenotypic responses on Margot or 90625 lines: resistance to aphids and to the virus when inoculated by aphids, susceptibility for both phenotypes, and susceptibility to aphids and resistance to the virus when inoculated by aphids; we did not characterize any clones revealing a melon line resistant to aphids and susceptible to virus when inoculated by aphids. Our results revealed a specifie interaction between plant resistance alleles in melon and aphid clones. The Vat gene belongs to the CC-NBS-LRRresistance gene (Pauquet et al 2004). The avirulence factor in aphid, expected to interact with the Vat protein, is presently unknown, it should be excreted in saliva and introduce in plant tissue during puncturing, and then interacts with the Vat product triggering defence responses (Dogimont et al 2010). These responses are expected to be efficient against aphids and viruses. Our results suggested two adaptation mechanisms of aphids to the Vat mediated resistance: i/ suppression of both phenotypes, i.e. susceptibility to aphids and to virus when inoculated by A. gossypii, what suggests a suppression of the recognition step (non-adapted avirulence product), ii/ susceptibility to aphids associated with resistance to virus when inoculated by aphids, suggested that the recognition step occurs, triggering the defence reponses that are efficient against virus but inefficient against aphids, i.e. aphids are adapted to these defence responses. We only observed adaptations of type i/ when considering the melon line 90625. Because the allele of resistance originating from this line has never been deployed in crops in areas we collected clones, these adaptations, observed in several clones, corresponded to an incomplete spectrum of the resistance efficiency. ln South-eastern France, the resistance that has been deployed is mediated by the allele at the Vat locus present in Margot. ln this area, where A. gossypii diversity is very large (Thomas et al 2012b), two clones, never observed before 2009, exhibited adaptation of type ii/ to that allele of resistance.
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Importance of the genetic background for sustainable resistance: experimental evidence for a major resistance gene to nematodes
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Arnaud Barbary, Alain Palloix, Ariane Fazari, Nathalie Marteu, Philippe Castagnone-Sereno, Caroline Djian-Caporalino
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France
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Root-knot nematodes, Meloidogyne spp., are extremely polyphagous plant parasites worldwide. Since the use of most chemical nematicides is being prohibited, genetic resistance is an efficient alternative way ta protect crops against these pests. However, resistance genes (R-genes) are limited and nematode populations are able ta overcome them with time. Good management of these valuable resources is thus a key point of R-gene durability. ln pepper, Me3 is a dominant major resistance gene, currently used in breeding programs, that control M. arenario, M. incognito and M. javanica, the three main root-knot nematodes species. ln this study, it was introgressed in either a susceptible or a partially resistant genetic background in either homozygous or heterozygous allelic status. Confronting these genotypes with a high inoculation pressure of an avirulent M. incognito isolate or a Me3 virulent laboratory-selected population (obtained by successive re-inoculation on a Me3 R-pepper line) demonstrated i) that the genetic background plays an important raie, Me3 being overcome more easily in a susceptible genetic background than in a partially resistant one, ii) that the allelic status has no effect. These results are in good agreement with concepts recently developed from the analysis of very different plant-pathogen interactions: pepper-virus (Palloix et al., 2009) or rapeseed-Leptosphaeria (Brun et al., 2012). Experiments are now underway ta detect and localise genes or loci providing partial resistance (QTLs = Quantitative Trait Loci) ta root-knot nematodes explaining the differences observed between susceptible and partially resistant genetic backgrounds, and ta determine the effectiveness of their « protective » raie on the major R-genes. Ali these results are of main importance for the creation of new varieties by breeders who have to take into account the plant material used and the resistance gene they want ta introgress.
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https://hal.inrae.fr/hal-02745433/file/Palloix%202012%20Plant%20resistance%20sustainability%2081_1.pdf BibTex
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Domestication and diversification of melon
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Michel Pitrat
article
Cucurbitaceae 2012, Proceedings of the Xth Eucarpia Meeting on Genetics and Breeding of Curcubitaceae, European Association for Research on Plant Breeding (EUCARPIA). FRA., Oct 2012, Antalya, Turkey
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https://hal.inrae.fr/hal-02744680/file/Pitrat%202012%20Eucarpia%2031-40_1.pdf BibTex
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Functional validation of the double phenotype triggered by the melon Vat gene by complementation experiments
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Veronique Chovelon, Veronique Restier, Nathalie Giovinazzo, Abdelhafid A. Bendahmane, Catherine Dogimont
article
10. EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, Oct 2012, Antalya, Turkey. 809 p
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Aphis gossypii is the only species of aphid colonizing melon, causing stunting and leaf curling and transmitting non-persistent viruses. We demonstrated previously by a map-based cloning strategy that a single CC-NBS-LRR gene, named Vat, confers the double resistance to A. gossypii colonization and to unrelated viruses when transmitted by this vector. We confirm here by complementation experiments, the double function mediated by the Vat gene. Two transgenes carrying the Vat gene under the transcriptional control of its own promoter or under the control of the CaMV-35S promoter respectively were inserted via Agrobacterium tumefaciens into the susceptible melon cultivar 'Védrantais'. Transgenic T2 plants were evaluated for their resistance to A. gossypii colonization and to Cucumber mosaic virus (CMV) transmission by this vector, in comparison with susceptible and resistant melon cultivars. Transgenic plants carrying both transgenes exhibited the Vat phenotype, restricting aphid colonization and CMV infection. The Vat gene under the control of its own promoter induced a high level of resistance, similar to the referent resistant cultivar 'Margot', whereas the gene under control of the CaMV-35S promoter induced a slightly weaker level of resistance.
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Resistance of melon to Cucumber Vein Yellowing Virus (CVYV)
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Michel Pitrat, Catherine Wipf-Scheibel, Didier Besombes, Cecile Desbiez, Hervé Lecoq
article
Cucurbitaceae 2012, Proceedings of the Xth Eucarpia Meeting on Genetics and Breeding of Curcubitaceae, European Association for Research on Plant Breeding (EUCARPIA). FRA., Oct 2012, Antalya, Turkey. 8 p
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https://hal.inrae.fr/hal-02748846/file/Pitrat_Besombes%202012%20Eucarpia%20157-164_1.pdf BibTex
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How microsatellite diversity helps to understand the domestication history of melon
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Laurana Serres-Giardi, Catherine Dogimont
article
10. EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, Oct 2012, Antalya, Turkey. 809 p
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Melon (Cucumis melo L.) population structure remains incomplete because of the sampling weakness in some botanical groups and for wild melons. The purpose of this study was to assess the genetic subdivisions in melon germplasm using a more representative sampling of the worldwide diversity and to investigate the localization of melon domestication and diversification. To reach this objective, a set of 20 microsatellite markers was used to genotype 713 accessions including 635 cultivated and feral melons, 66 wild melons and 12 relative Cucumis sp. A two-level structure in melon was revealed using population genetics statistics, clustering methods with genetic distances and Bayesian assignment method. Accessions split into two groups, fitting very well the subspecie melo and agrestis. Agrestis group was clearly substructured according to geographical origins, with African, Far-Eastern and Indian subgroups. The substructure of melo group was less resolute and the four clusters obtained grouped together several botanical groups originating from Europe, America, Middle East or Central Asia. African and Asian wild Cucumis melo were assigned to two distinct clusters in agrestis group, each with cultivated melons from the same respective geographical origin, suggesting that melon was domesticated twice. The domestication of wild African melon would have had a weak impact on the diversification of cultivated melon producing only Sudanese tibish and seinat types. The domestication of wild Asian melon, probably in india, would have first produced Indian cultivated melons and then be at the origin of Far-Eastern melons. Introduction of Eastern melons in the West and successive breeding activities may have then produced melo ssp. These findings are consistent with recent data on the Cucumis genus structure and origin, and with the high frequency of resistance genes found in melon Indian accessions.
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Inheritance of andromonoecy in Tibish
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M.E. Abdelmohsin, A.E. Eljack, E.A. Ahmed, Michel Pitrat
article
Cucurbitaceae 2012, Proceedings of the Xth Eucarpia Meeting on Genetics and Breeding of Curcubitaceae, European Association for Research on Plant Breeding (EUCARPIA). FRA., Oct 2012, Antalya, Turkey
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https://hal.inrae.fr/hal-02748609/file/Pitrat%202012%20Eucarpia%20427-431_1.pdf BibTex
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Domestication bottleneck as a signature of history diffusion of apricot species
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Hedia Bourguiba, Lamia Krichen, Neila Trifi-Farah, Bouchaib Khadari, Ali Mamouni, Samia Trabelsi, Claudio d'Onofrio, José Egea-Caballero, Bayram-Murat Asma, Jean Marc Audergon
article
6. Rosaceae Genomic Conference, Sep 2012, Mezzocorona (Trente), Italy
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Domestication generally implies a loss of diversity in crop species relative to their wild ancestors because of genetic drift through bottleneck effects. The comparison of genetic diversity in Mediterranean areas was assessed. 207 native apricot accessions representatives of the local variability from different Mediterranean countries: Algeria, France, Italy, Morocco, Spain, Tunisia and Turkey in each country were selected excluding those issued from breeding programs. This material was analysed for genetic diversity and structure using a common set of 25 monolocus microsatellites distributed throughout the Prunus genome. According to the geographic origin of the material and using a model‐based clustering method, four main gene pools were revealed, namely ‘Irano‐Caucasian’, ‘North Mediterranean Basin’, ‘South Mediterranean Basin’, and a more transversal one named ‘Adapted Diversity’. A significant gradient of decreasing diversity and allelic richness from the east to the south‐west of the Mediterranean Basin was highlighted. Overall, results suggested that from the Irano‐Caucasian area, apricot was introduced into the Mediterranean Basin through at least two different routes: the first one through the South of Europe and the second one through the North Africa. On the present bases relevant elements have been obtained useful for future management of apricot genetic resources as well as for genetic selection programs related to adaptive traits.
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Genetic variability description in a wide germplasm of domesticated peach through high throughput genotyping
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Diego Micheletti, Valeria Aramini, Pere Arus, Elisa Banchi, Teresa Barreneche, Daniele Bassi, Elisabeth Dirlewanger, Zhongshan Gao, Laura Gazza, Patrick Lambert, Francois Laurens, Xiongwei Li, Thierry Pascal, Bénédicte Quilot-Turion, Laura Rossini, Michela Troggio, Ignazio Verde, Maria Jose Aranzana
article
6. Rosaceous Genomics Conference, Sep 2012, Mezzocorona, Italy. 1 p
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Peach (Prunus persica (L.) Batsch) is one of the most economically important fruit crops in temperate areas. Classical fruit tree breeding is generally slow and inefficient. Molecular markers could improve its efficiency but, although nowadays many Mendelian traits are mapped in peach and SSR markers have been found to be linked to some of the key major genes, its use in breeding programs is still limited. Main reasons for that are insufficient linkage between the markers and the genes and the lack of markers suitable for medium‐high degree of multiplexing. To address this limitation, about 1,300 peach cultivars were genotyped with the 9K peach SNP chip (Verde et al. 2012) in the frame of FruitBreedomics project. This germplasm was chosen to be representative of the genetic diversity present in five germplasm collection in Europe and in China. Out of the 8144SNPs present in the chip, about 4300 were positively genotyped and used for the further analysis. The average number of heterozygous loci in the genotyped accessions was 1186 (spanning from 13 to 2775). The preliminary results of the population structure reveal three main subpopulations and the presence of high number of admixed individuals. LD seems to decay at distance longer than ca. 1 Mb. These results will be instrumental for implementing LD‐based mapping of QTLs and genes in peach.
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https://hal.science/hal-01209951/file/RGC6_Book%20of%20abstracts_FULL_web_1.pdf BibTex
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Diversité génétique de la réactivité des variétés aux stimulateurs de défenses des plantes (SDP), oïdium du melon et mildiou de la laitue.
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Brigitte Maisonneuve, Caroline Allen-Aubouard, Michel Pitrat
article
Rencontre technique Agriculture biologique légumes, Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL). FRA. Institut Technique de l'Agriculture Biologique (ITAB), FRA., Sep 2012, Lanxade, France
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The Ma gene for complete-spectrum resistance to root-knot nematodes in Prunus: biological features, positional cloning and validation
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Daniel Esmenjaud, Cyril van Ghelder, Joel Polidori, Henri Duval, Marc M. Kleinhentz, Elisabeth Dirlewanger
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30. Symposium of the European Society of Nematologists, Sep 2012, Adana, Turkey
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Plus fort, plus loin, plus lent : Comment le fond génétique de la plante peut augmenter la durabilité d’un gène majeur de résistance
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Julie Quenouille, Josselin Montarry, Alain Palloix, Benoît Moury
article
34. Réunion annuelle du Groupe d’Etude de Biologie et Génétique des Populations (Petit Pois Déridé 2012), Aug 2012, Avignon, France
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La création et l’utilisation de variétés de plantes hôtes résistantes aux parasites est le moyen de protection des cultures le plus respectueux des ressources naturelles et de l’environnement. Cependant ces résistances perdent parfois leur efficacité du fait de l’adaptation des bioagresseurs ciblés. Il a récemment été montré, pour trois pathosystèmes différents, que les résistances polygéniques combinant un gène majeur de résistance et un fond génétique partiellement résistant sont plus durables que les résistances monogéniques (le même gène majeur dans un fond génétique sensible). En utilisant le pathosystème virus Y de la pomme de terre (PVY)/piment ([i]Capsicum annuum[/i]), nous avons analysé trois mécanismes qui pourraient expliquer la meilleure durabilité des résistances polygéniques : (i) L’effet, sur l’accumulation virale, de la résistance partielle conférée par le fond génétique, (ii) l’augmentation du nombre de mutations nécessaire pour contourner la résistance et (iii) le ralentissement de la sélection des mutants contournant la résistance. Ces trois mécanismes ont été validés expérimentalement. Le premier mécanisme explique environ 75% de la variation de la fréquence de contournement du gène de résistance pvr23 et est, par conséquent, un déterminant majeur de la durabilité de la résistance. Le fond génétique de la plante a également une influence sur le deuxième mécanisme en complexifiant les voies mutationnelles que le virus peut emprunter pour contourner la résistance et sur le troisième mécanisme en ralentissant la sélection des variants viraux les mieux adaptés. Ces travaux fournissent des connaissances sur les mécanismes biologiques responsables de la meilleure durabilité de la résistance polygénique avec comme objectif de préserver la durabilité des gènes majeurs de résistance exploités dans les programmes de sélection.
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https://hal.science/hal-01208595/file/2012-Quenouille-PPD%20colloque_1.pdf BibTex
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African eggplants and nightshades: an overview of indigenous vegetables
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Marie-Christine Daunay, Anne-Laure Girard
article
9. Solanaceae Conference (SOL2012), Aug 2012, Neuchâtel, Switzerland
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https://hal.inrae.fr/hal-02745546/file/Daunay%202012%20SOL2012%20110_1.pdf BibTex
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Identifying a candidate gene for the broad-spectrum resistance qtl to Phytophthora root rot and blight in pepper
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Véronique Lefebvre, Céline Vandecasteele, Melissa Cantet, Stéphane Mallard, Sonia S. Vautrin, Abdelhafid A. Bendahmane, Helene H. Berges
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9. Solanaceae Conference (SOL2012), Aug 2012, Neuchâtel, Switzerland
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https://hal.inrae.fr/hal-02746226/file/Lefebvre_Cantet%202012%20SOL2012%2097_1.pdf https://hal.inrae.fr/hal-02746226/file/poster_lefebvre_2.pdf BibTex
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Molecular genetic analysis of floral meristem diversity in tomato
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Guillaume Bauchet, Stephane Munos, Christopher Sauvage, Julien Bonnet, Laurent Grivet, Mathilde M. Causse
article
9. Solanaceae Conference (SOL2012), Aug 2012, Neuchâtel, Switzerland
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https://hal.inrae.fr/hal-02746318/file/Bauchet_Sauvage%202012%20SOL2012%2043_1.pdf BibTex
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Contribution to phenomics for quantitative disease resistance in a germplasm collection of Capsicum spp.: a multivariate analysis by self-organizing map
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Melissa Cantet, Guillaume Marie, Anne-Marie Sage-Palloix, Anne Massire, Alain Palloix, Véronique Lefebvre
article
9. Solanaceae Conference (SOL2012), Aug 2012, Neuchâtel, Switzerland. 250 p
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Impact of temperature integration under greenhouse on energy use efficiency, plant growth and development and tomato fruit quality (sugars, acids, vitamin C, carotenoids) depending on variety rootstock combination
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Vincent Truffault, Fatiha Fifel, Hélène Gautier, Gilles G. Vercambre, Serge Le Quillec, Jean Jacques Longuenesse
article
2. Symposium on Horticulture in Europe, Jul 2012, Angers, France. 7 p
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The present study on soil-less tomato crops aims to evaluate and compare on contrasted genotypes the impact of temperature integration (TI) on plant growth and development as well as on fruit yield and quality. TI, taking advantage of plant plasticity regarding temperature, consists in modulating the set points for heating (lower during the night) and ventilation (higher during the day) keeping similar mean 24h temperature for plant development. Four variety-rootstock combinations of tomato plants were studied for two consecutive years (2010 and 2011). Two climate managements were compared: a temperature integration technique (TI) and a control (grower’s technique). In order to test the limit of TI system, TI application lasted longer in 2011 and with temperature settings colder compared to 2010. During the first year of experiment (2010), energy saving was about 22% during TI period. Yield was not modified, nor leaf growth. In 2011, TI led to an 8.6% energy saving compared to control. Yield significantly increased for Plaisance-Beaufort combination and was not affected in other variety-rootstock combinations. In conclusion, for both years TI increased energy use efficiency. Plant development rate was not significantly modified by TI. Leaf area slightly decreased (-11%). Fruit size and composition (sugars, acids, vitamin C) were not modified by TI. Carotenoids (specifically phytoene and lycopene) were improved by TI in particular for Plaisance-Beaufort; this might be related to the reduced leaf area that allowed more light to reach the fruit and consequently promoted carotenoid synthesis. Temperature integration appears as a profitable alternative to classic management of greenhouse climate.
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A multi-Ievel omic approach of tomato fruit quality
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Jiaxin Xu, Laura Pascual, Nelly Desplat, Mireille Faurobert, Yves Y. Gibon, Annick Moing, Mickaël Maucourt, Patricia Ballias, Catherine Deborde, Yan Liang, Jean-Paul Bouchet, Dominique D. Brunel, Marie-Christine Le Paslier, Mathilde M. Causse
article
2. Symposium on Horticulture in Europe, Jul 2012, Angers, France
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Tomato fruit quality is an important trait for tomato consumers, but complex to improve due to the number of components involved and by their polygenic nature. ln order to decipher the genetic diversity and the inheritance of fruit qua lity components at a global level, we conducted a large multi-Ievel omic experiment. A set of 8 contrasted lines and 4 of their F1 hybrids were phenotyped for fruit development traits. Fruits were harvested and pericarp samples analysed at 2 stages (cell expansion and orange) and different scales: (1) primary and secondary metabolome profiles, (2) activities of 28 enzymes involved in primary metabolism, (3) proteome profiles revealed by 2D-PAGE and sequencing of 470 spots showing quantitative variations and (4) gene expression analysis by Digital Gene Expression. ln parallel, the 8 lines were resequenced and more than 3 millions SNPs identified when aligned on the reference tomato genome. This experiment allowed us to address several questions: the range of variability for the metabolic traits and expression data. Correlation networks can be constructed within and between levels of analysis to identify regulatory networks. Diversity of chosen candidate genes can be analysed, relating the polymorphisms at the sequence levels with their expression. Some examples will be presented.
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https://hal.inrae.fr/hal-02748459/file/Faurobert_Bouchet%202012%20SHE%20294_1.pdf BibTex
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Evolution of diversity of fruits and vegetables crops
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Michel Pitrat, Jean Marc Audergon
article
2. Symposium on Horticulture in Europe, Jul 2012, ANGERS, France
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We will answer only to a part of the session title (Horticulture and biodiversity: contribution to its loss, conservation or increase?) which includes the impact of the cultivars and cultivation methods on the biodiversity. Our presentation will focus on the diversity of fruits and vegetables crops in Europe at the species, cultigroup and cultivar levels and its evolution with time. Due to climatic conditions and cultural traditions, only a part of the world fruit and vegetable diversity is cultivated in Europe. Until the Second World War, most of the cultivars were locally based. Since the 1950', major evolutions have affected most of the cultivated species at several levels:
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https://hal.inrae.fr/hal-02746535/file/Pitrat_Audergon%202012%20SHE%20230_1.pdf BibTex
titre
Selection pressure exerted by host resistance genes shapes the population genetic structure of the lettuce downy mildew, Bremia lactucae, in France
auteur
Romain Valade, Aurélie Ducasse, Brigitte Maisonneuve, Claire Neema
article
8° Colloque de la Société Française de Phytopathologie (SFP), Societe Francaise de Phytopathologie (SFP). Paris, FRA., Jun 2012, Paris, France
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absent
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Compromising NSGA-II performances and stopping criteria: case of virtual peach design
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Mohamed Ould Sidi, Abdeslam Kadrani, Bénédicte Quilot-Turion, Francoise F. Lescourret, Michel M. Génard
article
International Conference on Metamaterials, Photonic Crystals and Plasmonics, Apr 2012, Paris, France. 2 p
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Multiobjective evolutionary optimization algorithms allow exploring highly dimensional search (decision) spaces in a reasonable computation time. These methods provide the decision-maker with a set of diversified solutions. The decision-maker will thus have the final choice of the best suited trade-off between criteria. However, a difficulty when using these algorithms is the convenient time to stop computation. Generally, one uses a predefined maximum number of generations as stopping criterion. Unfortunately, this can lead to many unnecessary generations and thus decrease the ratio of solution quality by computation time. Authors of [4] discussed this aspect, and illustrated it with the well-known NSGA-II (Non-dominated Sorting Genetic Algorithm II)[2]. They introduced a new stopping criterion which may partly remedy to this drawback. This criterion consists of stopping the process when the algorithm has performed a given number of iterations without improvement. It is based on the standard deviation of the crowding distance, a diversity control measure of NSGA-II, during a given number of iterations. Authors have tested their criterion only in the case of unconstrained and bi-objective problems and they claimed that it is difficult to use this criterion with NSGA-II for more than 2 criteria and for constrained optimization problems. Consequently, the question we address here is the possibility to use this stopping criterion to improve NSGA-II computation time in case of a constrained three-objective optimization problem. Thus, we compared the results obtained with the original version of NSGA-II to those obtained when modifying this original version by including this stopping criterion. In both studies we used the ‘Virtual Fruit’, a process-based model [3]. Six genetic parameters of the model constitute the decision variables of our problem and will be combined to create the genotypes by optimization. Three traits of fruit quality and sensitivity to brown rot simulated by the model were taken into account to evaluate the solutions (genotypes). Six constraints were also considered. The population size, 400, and the number of generations, 1500, were chosen after performing many preliminary simulations with the original version of the NSGA-II algorithm (data not shown). In order to choose the best values of the two parameters involved in the stopping criterion (L the length of the time window and the threshold value δ lim) adapted to the population size considered, we tested different combinations of values (20, 40, 60, and 80 generations for L combined with values of 0.001, 0.005, 0.0075, and 0.0001 for δ lim). Values of 0.001 and 20 were selected respectively for the threshold and length of time window. We repeat simulations of both versions 13 times. We compared the performances of the two versions using standard performance metrics and statistical analysis. Coverage set, spacing, and dominated volumes ration metrics[1] were used together with the computational time to compare the performances of the original (or) and the modified (sd: standard deviation) versions of NSGA-II. Results are given in table 1. We concluded that the NSGA-II with the stopping criterion based on the standard deviation find very similar results compared to the original NSGA-II but in less computational time.
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Méthodes multicritères pour le tri de fruits virtuels
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Mohamed-Mahmoud Ould-Sidi, Bénédicte Quilot-Turion, Antoine C. Rolland
article
13. Congrès Annuel de la Société Française de Recherche Opérationnelle et d’Aide à la Décision, Apr 2012, Angers, France. 2 p
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https://hal.inrae.fr/hal-02811362/file/ROADEF_2012_MMOS_BQT_AR_1.pdf BibTex
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A generic tool for managing and exploiting genetic association studies results using high throughput genotyping and phenotyping
auteur
Célia Michotey, Frédéric Sapet, Pierre Dubreuil, Jacques Le Gouis, Mathilde Causse, Cyril Pommier, Sophie Durand, Nacer Mohellibi, Raphaël Flores, Catherine Delaitre, Stephane Nicolas, Alain Charcosset, Yannick de Oliveira, Patrice This, Roberto Bacilieri, Alexis Dereeper, Philippe Lashermes, Hadi Quesneville, Delphine Steinbach
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Plant Genomics Seminar 2012, Apr 2012, Pont-Royal en Provence, France
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Identification of a candidate gene for the key broad-spectrum resistance QTL to late blight in pepper
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Véronique Lefebvre, Helene H. Berges, Abdelhafid A. Bendahmane
article
Plant Genomics Seminar 2012, Apr 2012, Pont-Royal en Provence, France
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Determining the gene responsible for a QTL controlling broad-spectrum resistance to pathogens Phytophthora agents are the most devastating pathogens of Dicotyledons. Chemical controls against these pathogens are insufficiently effective, and have serious consequences on the environ ment and human health. An alternative more environmentally friendly is the use of pathogen-resistant varieties. However, monogenic resistances are rare and often specifie to a strain of the pathogen (narrow spectrum); they are thus frequently overcome because of the emergence of new virulent strains. Polygenic resistances have often the advantage of being broad-spectrum. ln addition, they have been demonstrated for some of them more durable. But their use in selection is limited because the controlling loci (named QTLs for Quantitative Trait Loci) are difficult to identify due to their number and their partial individual effect on resistance. ln addition, it is not known in what resistance QTLs differ from monogenic resistance genes. Consequently, we aim to determine the molecular basis of a QTL involved in polygenic resistance to Phytophthora capsici, the agent of late blight in pepper (Capsicum annuum). From the fine genetic map to the identification of a candidate gene: chromosome walking, seguencing and gene annotation The QTL Phy-PS confers a high level of broad-spectrurn resistance towards P. capsici. Identified in several resistant genitors of C annuum, it was mapped to chromosome P5. To facilitate its fine mapping, we derived a population that segregates for the QTL alone. Then a physical map of the locus was constructed by chromosome walking based on two BAC libraries (BACfor Bacterial Artificial Chromosome), and anchored to the fine genetic map. BAC clones covering the locus have been sequenced by classical and new generation sequencing approaches. An automatic annotation pipeline has been adapted to the pepper genome to characterize sequence repeats that are very common in this species, and to predict the coding sequences corresponding to hypothetical functional genes. An analysis of nucleotide and protein homology and a search for protein domains have been achieved to identify among them candidate genes. Finally, severa 1 methods for functional validation have been considered and developed to study the role of candidate genes in the resistance to P. capsici.
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Valorisation of genetic and genomic resources of tomato for the improvement of fruit quality
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Mathilde M. Causse, Dominique D. Brunel, Paul Amouroux
article
Plant Genomics Seminar 2012, Apr 2012, Pont-Royal en Provence, France
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ln this project we intend to develop the resources necessary for high throughput QTL studies in tomato by combining the development of genomic and plant resources including: (1) a large set of SNPs in expressed sequences and flanking regions based on high throughput sequencing technology. (2) a set of 4 Illumina VeraCode chips carrying a total of 1536 SNP (3) Creation and phenotype characterisation of a multiparent advanced generation intercross (MAGIC) population to be used for QTL mapping and latter for fine mapping, as a complement to genetic resources gathered in collections. The population will be characterised for yield related traits and fruit quality (composition in sugars and acids, colour, texture). (4) Differentiai Gene Expression profiles and proteome profiles of the 8 parental lines of the MAGIC populations and the 4 Fl hybrids These resources are developed to be used in the subsequent analyses: (1) Genotyping a biallelic population in order to precisely map ail the markers present on the VeraCode chips (2) Genotypinç the MAGIC population with the Illumina VeraCode chips in order to map QTL (3) Genotyping several genetic resources (collections of cherry tomato cultivars, of old cultivars and a set of elite genotypes), in order to assess linkage disequilibrium and search for associations between SNP markers and fruit related traits (4) Expression profile analysis of the genes and proteins characterised at the 2 stages of expression; comparison among genotypes and Fl hybrids. The final expected result is a genetic map of more than 1000 SNPs which will be associated with QTL locations and expression variations of the parental lines and Fl hybrids.
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https://hal.inrae.fr/hal-02749148/file/Causse%202012%20Genoplante%2085-86_1.pdf BibTex
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Molecular basis of virus resistance mediated by host factors required for the infectious cycle
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Carole Caranta, S. Lacombe, Sophie Ewert, Jean-Luc J.-L. Gallois, Laurence Ouibrahim, André Moretti, Joan Estevan, L. Albar, A. Ghesquiere, J. Bouniol, D. Thiemele, E. Hébrard, D. Fargette, F. Vignols, N. Poulicard, Amal Moumène, Emilce Prado, Monique M. Beuve, Vincent Dumas, Veronique Komar, Emmanuelle Vigne, Gerard Demangeat, Olivier O. Lemaire, Didier Merdinoglu
article
Plant Genomics Seminar 2012, Apr 2012, Pont-Royal en Provence, France. 2 p
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ln recent year, one of the most interesting results that has enabled plant virology to make a significant step forward is the identification of components of the translation initiation complex as essential host factors required for RNA virus multiplication. Although translation initiation factors were demonstrated to be highly conserved determinants of plant resistance to viruses, preliminary data indicate that the molecular basis underlying translation initiation factors-mediated resistance are highly variable. ln parallel, several recessive resistance genes against viruses were identified and demonstrated to be distinct from translation initiation factors. These genes are therefore very good candidates for the discovery of new susceptibility factors. ln this context, the MOVle project airns at (i) the characterization of mechanisms underlying molecular specificity of translation initiation factors mediated resistance, (ii) the study of the potential role of translation initiation factors in RNA virus resistance in economically important crops such as grapevine, and (iii) the identification of new host factors required for viral infection The project is likely to provide fundamental insights into the molecular basis of plant-virus interactions and to greatly facilitate the exploitation of host factors required for the viral cycle as targets to improve plant resistance to viruses.
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https://hal.inrae.fr/hal-02746501/file/Caranta_Gallois%202012%20Genoplante%2098-100_1.pdf BibTex
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Variation in tomato fruit ascorbate levels and consequences of manipulation of ascorbate metabolism on drought stress tolerance
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Vincent Truffault, Noé Gest, Cecile Garchery, Mathilde M. Causse, Renaud Duboscq, Gisèle Riqueau, Christopher Sauvage, Hélène Gautier, Pierre Baldet, Rebecca Stevens
article
2. International Symposium on Biotechnology of Fruit Species, Mar 2012, Nelson, New Zealand
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Tomato is an important crop worldwide and one of the major sources of vitamin C (ascorbate) in the human diet. Ascorbate contributes both to tomato nutritional quality and has roles in stress tolerance and adaptation to the environment. In this study we show the variability that exists in tomato germplasm in terms of ascorbate content (10 to 90 mg/100 g fwt) which could be starting point for evaluating correlations with physiological traits potentially linked to ascorbate. We have then manipulated genes involved in ascorbate metabolism, using RNA interference, to investigate their influence on fruit ascorbate levels, fruit physiology and yield under both normal and drought stress conditions. There is some evidence that one of the genes chosen affects fruit size and yield under different conditions, but clear effects on the fruit ascorbate pool are not seen. We conclude that ascorbate metabolism is complex and can also have wider effects on fruit physiology and growth.
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Modifications of antioxidant enzymes as a tool to improve yield in tomato under water deficit
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Noé Gest, Cecile Garchery, Mathilde M. Causse, Hélène Gautier, Pierre Baldet, Rebecca Stevens
article
2nd International Symposium on Biotechnology of Fruit Species, Mar 2012, Nelson, New Zealand. 228 p
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https://hal.inrae.fr/hal-02745000/file/Stevens%202012%20Biotech%20fruit_1.pdf https://hal.inrae.fr/hal-02745000/file/Stevens%202012%20Biotech%20fruit_2.doc BibTex
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L'abricot, fruit d'un terroir. La diversité de l'abricot, une richesse attachée au terroir
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Jean Marc Audergon, Eric Martin, Alain Blanc, Frederic Gilles, Anne Marie Ferréol, Guy Clauzel, Laurent Brun, Vincent Mercier, Barbara Gouble, Sylvie Bureau, Catherine M.G.C. Renard, Claire Lamine, Véronique Decroocq, Thierry Candresse, Guillaume Roch
article
Abricot de Rhône-Alpes, une stratégie d'avance!, Mar 2012, Mercurol, France
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Apricot susceptibility to blossom brown rot (Monilia spp.) and leaf rust (Tranzschelia spp.) under low-input production system
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Claude Eric Parveaud, Jm Broquaire, Vincent Mercier, Christelle Gomez, Laurent Brun, Guy Clauzel, Armand Guillermin, Jean Marc Audergon
article
15. international conference on organic fruit-Growing, Feb 2012, Hohenheim, Germany. 427 p
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Disease-susceptibility of apricot cultivars is a key-stone towards low-input production systems. Among the main diseases, the susceptibility to Monilinia spp. and leaf rust of apricot cultivars are economically important but rarely assessed. A 5-years study in two sites was conducted to assess the susceptibilities to Monilinia spp. and leaf rust of 16 apricot cultivars under natural conditions (no inoculation). No fungicide and insecticide were applied during the experiment. In Torreilles site, no significant Monilinia spp. was observed. In Gotheron site, the percentage of shoots necrosed by Monilinia spp. ranged from 13% to 100% during 2010-2011. A Genotype x Environment interaction was observed for Monilinia spp. damages. Concurrently a high variability to leaf rust was observed in both sites. The ranking of cultivars susceptibility to leaf rust was similar between both sites
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https://hal.inrae.fr/hal-02746309/file/Apricot_ssceptibility_Conference%20Organic%20Fruit_1.pdf BibTex
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Peach molecular breeding at the INRA Avignon and links to a new European initiative to increase fruit breeding efficiency: FP7 KBBE FruitBreedomics
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Patrick Lambert
article
1. International Congress for Bacterial Diseases of Stone Fruits and Nuts, Feb 2012, Zurich, Switzerland. 28 p
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Genetic diversity of Phytophthora capsici recovered from pepper in Southern France
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D. Gobena, Melissa Cantet, Véronique Lefebvre, K. Lamour
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2012 APS Southern Division Meeting, Feb 2012, Birmingham, Alabama, United States
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Is apple powdery mildew the causal agent of peach rusty spot?
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Levente Kiss, Tünde Jankovics, Nenad Dolovac, Aleksandra Bulajic, Branta Krstic, Thierry Pascal, Marc Bardin, Philippe C. Nicot
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22. Keszthelyi Növényvédelmi Fórum (22nd Plant Protection Forum), Jan 2012, Keszthely, Hungary. 85 p
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Populations de Bremia Lactucae en Provence
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Brigitte B. Maisonneuve, Sandrine S. Jeuniaux, Emilie E. Juillard, Marion M. Lovera, Jérémy J. Gaudin, Romain R. Valade, Claire C. Neema
article
9. Rencontres de Phytopathologie-Mycologie de la Société française de Phytopathologie (SFP), Jan 2012, Aussois, France. pp.5.11
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absent
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https://hal.science/hal-01000208/file/49307_20120116092127326_1.pdf BibTex
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Application of high throughput genotyping techniques in peach germplasm and breeding lines within the fruitbreedomics project
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Diego Micheletti, Valeria Aramini, Pere Arus, Elisa Banchi, Teresa Barreneche, Daniele Bassi, Elisabeth Dirlewanger, Zhongshan Gao, Laura Gazza, Patrick Lambert, Francois Laurens, Xiongwei Li, Thierry Pascal, Bénédicte Quilot-Turion, Laura Rossini, Michela Troggio, Eric van de Weg, Ignazio Verde, Maria Jose Aranzana
article
20. Plant and Animal Genome, Jan 2012, San Diego, United States. 1 p
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Peach (Prunus persica (L.) Batsch) is one of the most economically important fruit crops in temperate areas. Every year new varieties are released from breeding programmes trying to meet the requirements imposed by the market. Classical fruit tree breeding is generally slow and inefficient. Molecular markers could improve its efficiency but, although nowadays many Mendelian traits are mapped in peach and SSR markers have been found to be linked to some of the key major genes, its use in breeding programmes is still limited. Main reasons for that are insufficient linkage between the markers and the genes and the lack of markers suitable for medium-high degree of multiplexing. FruitBreedomics is a European project conceived with the strategic goal to improve the efficiency of fruit breeding by bridging the gap between scientific knowledge and application in breeding. In the frame of this project, and with the goal of obtaining SNP closely linked to agronomic interesting traits, the 9K peach SNP chip (Illumina Inc.) developed by the International Peach SNP Consortium (IPSC) has been used in both segregating populations and in germplasm collections. This chip, containing 8144 SNPs scattered on the 8 peach chromosomes, has been used in about 1,500 individuals belonging to seventeen Prunus crosses (12 peach x peach and 5 interspecific) and in 1,300 peach cultivars from different origins, including some of the founders of the current breeding programs
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Genetic diversity, inheritance and integration of multi-level omic data in tomato fruit
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Jiaxin Xu, Laura Pascual-Banuls, Nelly Desplat, Jean-Paul Bouchet, Yves Y. Gibon, Annick Moing, Dominique D. Brunel, Marie-Christine Le Paslier, Mathilde M. Causse
article
20. Plant and Animal Genome, Jan 2012, San Diego, United States. pp.W643
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Tomato fruit quality is an important trait for tomato breeders, but complex by the number of components and by their polygenic nature. In order to decipher the genetic diversity and the inheritance of fruit quality components at a global level, we conducted a large multi-level omic experiment. A set of 8 contrasted lines and 4 of their F1 hybrids were phenotyped for fruit development traits. Fruits were harvested and pericarp samples analysed at 2 stages (celle expansion and orange) and different scales: (1) primary and secondary metabolome profiles, (2) activities of 28 enzymes involved in primary metabolism, (3) proteome profiles revealed by 2D-PAGE and sequencing of 470 spots showing quantitative variations and (4) gene expression analysis by Digital Gene Expression. In parallel, the 8 lines were resequenced and more than 3 millions SNPs identified when aligned on the reference tomato genome. This experiment allowed us to address several questions: the range of variability for the metabolic traits and expression data. Correlation networks can be constructed within and between levels of analysis to identify regulatory networks. Diversity of chosen candidate genes can be analysed, relating the polymorphisms at the sequence levels with their expression. Some examples will be presented
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The new EU project FruitBreedomics: an integrated approach for increasing breeding efficiency in fruit tree crops
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Francois Laurens, M.J. Aranzana, P. Arus, D. Bassi, J. Bonany, L. Corelli, Charles Eric Durel, J. Mes, Thierry Pascal, A. Patocchi, A. Peil, Bénédicte Quilot-Turion, S. Salvi, S. Tartarini, M. Troggio, A. Vecchietti, R. Velasco, E. van de Weg
article
XX. PAG Plant & Animal Genome, Jan 2012, SanDiego, United States. 8 p
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The european fruit industry is facing economic challenges imposed by increasingly fierce international competition and decreasing fruit consumption, societal demand for a more sustainable production, and biological problems caused by climate changes. Releasing new cultivars that meet these challenges is a major goal of all european breeding programmes. However, addressing them has been slow due to the nature of fruit tree breeding: long term, low efficiency and high cost. For the past 15 years, efficient networks of fruit geneticists and genomicists have progressively been built thanks in particular to EU-funded projects. Europe has thus become a leader in research on fruit genetics aimed at enhancing fruit quality traits as well as resistance to biotic stresses. European teams working on fruit genetics have developed up-to-date tools and skills covering most of the "omics" fields as well as statistics and software development. Although fruit breeding is very active in Europe, very few breeding programmes are really using the output of the fruit genetics/genomics research. A few bottlenecks can explain this situation. To solve that, a new european initiative has been set up by the fruitbreedomic. This large collaborative project, has the strategic goal of improving the efficiency of current fruit breeding programmes by bridging the existing gap between molecular genetics research and application in breeding.
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Sensibilité variétale de l'abricotier au Monilia sur fleur, à la tavelure et à la rouille
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Laurent Brun, Vincent Mercier, Jm Broquaire, Christelle Gomez, Claude Eric Parveaud, Guy Clauzel, Armand Guillermin, Jean Marc Audergon
article
Rencontre Phytosanitaire Fruits à Noyau, Jan 2012, Balandran, France. 11 p
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Infrared spectroscopy: a rapid tool for apricot characterization.
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Sylvie Bureau, Marielle Boge, Catherine C. Renard, Jean Marc Audergon
article
International Symposium on Apricot Breeding and Culture, 2012, Yerevan, Armenia
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Influence of temperature on apricot fruit respiration and ethylene production.
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Barbara Gouble, Patrice Reling, Catherine C. Renard, Jean Marc Audergon
article
International Symposium on Apricot Breeding and Culture, 2012, Yerevan, Armenia
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Inheritance of phenological traits in apricot progenies.
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Jean Marc Audergon, Guy Clauzel, Alain Blanc, Guillaume Roch, Patrick Lambert, D. Ruiz, J.A. Campoy, J.A. Salazar, P. Martínez-Gómez, J. Egea, Sylvie Bureau, Barbara Gouble, Marielle Boge, Patrice Reling, Catherine C. Renard, Luca Dondini, S. Tartarini
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15 th International Symposium on Apricot Breeding and Culture , 2012, Yerevan, Armenia
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Poster communications

titre
Mise en œuvre d’un système de surveillance des enceintes climatiques à l’INRA-PACA
auteur
Frédéric Jean, Olivier Marloie, Marie Noëlle Corre, Damien Gounelle, Mehdi Pringarbe
article
Séminaire AQR, Dec 2012, Nouan le Fuzelier, France. 2012
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https://hal.inrae.fr/hal-02809975/file/Poster_Surveillance_Alarme_Enceintes_UERFM_2012_V2_1 BibTex
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Protection des laitues contre le Bremia par des stimulateurs de défenses des plantes (SDP) diversité génétique de la réactivité des variétés.
auteur
Brigitte Maisonneuve, Anne Pesme-Glemin
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2012, Avignon, France. 2012
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Effects of lettuce cultivar mixtures, differing for resistance genes, on Bremia lactucae incidence.
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Brigitte Maisonneuve, Eric Martin, Loic Jean, Claude C. Pope de Vallavieille, Michel Pitrat
article
Plant resistance sustainability 2012. International conference, Oct 2012, La Colle sur Loup, France. 2012
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https://hal.science/hal-01190799/file/resumes_1.pdf BibTex
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High or low fructose? Consequences for sugar metabolism in peach fruit
auteur
Elsa Desnoues, Yves Y. Gibon, Valentina Baldazzi, Veronique Signoret, Michel M. Génard, Bénédicte Quilot-Turion
article
6. Rosaceous Genomics Conference, Sep 2012, San Michele all’Adige, Italy. 1 p., 2012
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https://hal.inrae.fr/hal-02804340/file/poster_RGC6_ED_1.pdf BibTex
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Identification of genomic regions and candidate genes involved in fruit ripening in two apricot (Prunus armeniaca L.) cultivars
auteur
Patrick Lambert, David Ruiz, Barbara Gouble, Sylvie Bureau, Luis Buendia, Maryse Reich, Patrice Reling, Catherine M.G.C. Renard, Jean Marc Audergon
article
6. Rosaceous Genomics Conference, Sep 2012, Mezzocorona, Italy. 2012
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https://hal.inrae.fr/hal-02807222/file/Lambert%202012%20Rosa.%20Geno.%20Conf.%20ITA_1.pdf BibTex
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What do spring migrants reveal about sex and host selection in the melon aphid
auteur
Sophie Thomas, Nathalie Boissot, Flavie Vanlerberghe-Masutti
article
34. Réunion annuelle du Groupe d’Etude de Biologie et Génétique des Populations (Petit Pois Déridé 2012), Aug 2012, Avignon, France. 2012
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https://hal.inrae.fr/hal-02805852/file/Thomas_Boissot%202012%20PPD%20Poster_1.ppt https://hal.inrae.fr/hal-02805852/file/Thomas_Boissot%202012%20PPD%20Poster_2.ppt BibTex
titre
Molecular marker development for introgression of nematode resistance in potato breeding programs: exploitation of [i]solanum spegazzinii[/i] resistance source
auteur
Laura Chauvin, Marie-Laure Lesage, Sylvain Fournet, Bernard Caromel, Josiane Quéré, Jean-Paul Dantec, Marie-Claire Kerlan
article
9. Solanaceae Conference (SOL2012), Aug 2012, Neuchâtel, Switzerland. 2012, SOL 2012: From the Bench to Innovative Applications
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https://hal.science/hal-01208704/file/Caromel%202012%20SOL2012%2044_1.pdf BibTex
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Comparison of metabolomic, proteomic and genomic variability in tomato fruit
auteur
Mathilde M. Causse, Jiaxin Xu, Laura Pascual, Yves Y. Gibon, Annick Moing, Nelly Desplat, Dominique D. Brunel
article
9. Solanaceae Conference (SOL2012), Aug 2012, Neuchâtel, Switzerland. 2012, SOL 2012: From the Bench to Innovative Applications
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https://hal.inrae.fr/hal-02745839/file/Causse_Xu%202012%20SOL2012%20147_1.pdf BibTex
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Identification of a genetic factor determining the durability of a plant major resistance gene and quantitative resistance to virus accumulation
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Julie Quenouille-Lederer, Estelle Paulhiac, Pascale Mistral, Ghislaine Nemouchi, Anne-Marie Sage-Palloix, Bruno Savio, Vincent Simon, Benoît Moury, Alain Palloix
article
15. International Congress on Molecular Plant-Microbe Interactions (IS-MPMI 2012), Jul 2012, Kyoto, Japan. , 2012, IS-MPMI 2012 XV International congress. Program and abstracts
resume
Genetic resistance provides efficient control of crop diseases but is limited by pathogen counteradaptation. The durability of the pvr23 allele, conferring resistance to Potato virus Y (PVY), was demonstrated to depend on the plant genetic background. In order to identify genetic factors affecting the durability of the pvr23 resistance, QTL mapping was performed using doubled-haploid (DH) lines issued from the F1 between two Capsicum annuum lines: ‘Perennial’ carrying pvr23 in a partially resistant background and ‘Yolo Wonder’ carrying the susceptible pvr2+ allele in a susceptible background. 350 DH lines were genotyped with 234 markers and the linkage map was established. The 156 DH lines carrying the pvr23 allele but segregating for the genetic background were evaluated for two traits: the breakdown frequency of pvr23 (following inoculation with a PVY clone nonpathogenic (avirulent) towards pvr23) and the PVY accumulation (following inoculation with a mutant of the previous PVY clone carrying a single mutation conferring pathogenicity towards pvr23). Genotypic variance was highly significant for the two traits with heritabilities of 0.76 and 0.47. One major QTL, explaining 29% of the variance of pvr23 breakdown frequency was identified on chromosome 3 and two QTLs, explaining 25% and 9% of PVY accumulation variation, were identified on chromosomes 3 and 6, respectively. Interestingly, the major QTL for the 2 traits mapped to the same region of chromosome 3. A putative pleiotropic effect affecting simultaneously the two traits, the underlying mechanism and the perspective in breeding for resistance durability will be discussed.
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Poster communications
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https://hal.inrae.fr/hal-02745421/file/2012-Quenouille-MPMI-Poster_1.pdf https://hal.inrae.fr/hal-02745421/file/2012-Quenouille-MPMI-Abstract_2.pdf BibTex
titre
Integrative approach of tomato fruit texture using multi-block analysis
auteur
Rémy Aurand, Mireille Faurobert, Mohamed Hanafi, Gérard Mazerolles, Raphaël Tisiot, Laurent Rosso, Brigitte Navez, Nadia Bertin
article
2. Symposium on Horticulture in Europe, Jul 2012, Angers, France. 2012
resume
Integrative approach of tomato fruit texture using multi-block analysis. 2. Symposium on Horticulture in Europe
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Poster communications
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https://hal.science/hal-01595152/file/poster_Aurand.R_SHE2012_1.pdf https://hal.science/hal-01595152/file/Integrative%20approach%20of%20tomato%20fruit%20texture_doc%20she2012_2.docx BibTex
titre
The use of a multi-locus mixed model approach for GWAS reveals associations for metabolic traits in the tomato, Solanum lycopersicum
auteur
Christopher Sauvage, Vincent Segura, Guillaume Bauchet, Rebecca Stevens, Thi Phuc Do, Alisdair Fernie, Mathilde Causse
article
4. International Conference on Quantitative Genetics: Understanding Variation in Complex Traits, Jun 2012, Edinburgh, United Kingdom. 2012, 4. International Conference on Quantitative Genetics: Understanding Variation in Complex Traits
resume
Genome-wide association studies have been successful in identifying genes involved in polygenic traits notably in crops, towards their improvement. We propose to apply in a major crop, such as tomato, a recently developed multi-Iocus mixed model as a general method for mapping complex traits in structured populations (Segura et al., 2012). Among major crops, tomato (Solanum Iycopersicum), is an highly valuable vegetable in the world for its health value (Causse et 201 0). SNP beadchip (Hamilton et al., 2012) are available and enable GWAS for traits of interest. Recently, a pilote study defined the optimal conditions for GWAS by using cherry tomato accessions (Ranc et al., 2012). ln our study, we examined a core collection of 180 tomato varieties composed of 20 wild a cessions (S.pimpinellifollium), 130 admixed accessions (S. cerasiforme) and 30 domesticated accessions (S. Iycopersicum). Multi-Iocus GWAS analysis was conducted using the MLMM package (Segura et al., 2012) with 7700 SNP markers and a set of sugar-related, Vitamin C-related and morphological traits as weil as a broad range of metabolites related to central carbon metabolism. The present study is the first one in tomato reporting associations for a large set of traits at the genome scale. We found significant associations for 89 loci with a total of 19 traits including fresh weight, sucrose, ascorbate dehydrogenase, malate or citrate, notably. Identified loci were also concordant with published quantitative trait loci (i.e. Malate), while new loci were identified (for Tocopherol). Moreover, several related metabolites, such as citrate and malate (both involved in Krebs cycle) displayed two identical associations. These results (1) provide a list of candidate loci to be functionally validated and (2) provide a powerful analytical approach for finding genetic variants that can be directly used for crop improvement and deciphering the genetic architecture of complex trait.
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Poster communications
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https://hal.science/hal-01268380/file/Sauvage%202012%20ICQG%20235_1.pdf BibTex
titre
Identification and validation of reference genes for normalization of transcripts levels in roots of potatoes infected by nematodes
auteur
Patricio Castro Quezada, Jawad Aarrouf, Michel Claverie, Eric Grenier, Marie-Claire Kerlan, Bruno Favery, Véronique Lefebvre, Bernard Caromel
article
5. Congrès de la Société Française de Phytopathologie, Jun 2012, Paris, France. 2012
resume
Potato cyst nematodes induce changes in gene expression after infecting the roots. For studying gene expression, reverse transcription quantitative real-time PCR (RT-qPCR) is now a standard method. However, for accurate quantication of transcripts, a set of internal control genes, stable under different experimental conditions, is necessary for transcript normalization.[br/] Very few experimental data on suitable reference genes are available for plant-nematode interactions. In this study, we tested eight potential candidate reference genes identied through a microarray study performed on the pathosystem Arabidopsis/Meloigodygne for normalizing levels of potato gene transcripts, after invasion by nematodes. The transcription proles of these genes were assessed by RT-qPCR. Samples used in these experiments were RNAs, issued from roots of infected and uninfected potato plants, carrying resistant or susceptible alleles against Globodera pallida, following a kinetic of four days. Analyses were performed using the geNorm and NormFinder softwares.[br/] Our results showed that the eight candidate reference genes were stably expressed in our conditions. For accurate normalization in RT-qPCR studies, RPN7 (26S proteasome regulatory subunit, involved in protein synthesis) and MST2 (mercaptopyruvate sulfurtransferase) were the top ranked genes and, therefore, are reliable reference genes in compatible and incompatible potato-nematode interactions.
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Poster communications
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https://hal.science/hal-01208737/file/Lefebvre_Caromel%202012%20SFP_1.pdf BibTex

Reports

titre
Rapport de fin de contrat de recherche - Contrat Resist-laitue : Analyse du mildiou de la laitue et mise en place de stratégies pour apporter des résistances durables
auteur
Brigitte Maisonneuve
article
[Contrat] AgroParisTech; Gautier semences; Rijk Zwaan. 2012
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titre
A tool to conveive sustainable production systems_case of the peach-brown rot couple
auteur
Abdeslam Kadrani
article
[Contract] 0902-011, 2012
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Reports
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https://hal.inrae.fr/hal-02805946/file/rapport%20final%20Quilot%20turion%202012%20Kadrani_1.pdf https://hal.inrae.fr/hal-02805946/file/rapport%20final%20Quilot%20turion%202012%20Kadrani_2.docx BibTex
titre
Analyse et évaluation de la durabilité d'une résistance partielle au Watermelon mosaic virus (WMV) chez le melon
auteur
Michel Pitrat
article
[Contrat] C07-2008, 2012
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2011

Conference papers

titre
Insect resistance in melon and its modification by molecular breeding
auteur
Catherine Dogimont, Nathalie Boissot
article
8. Joint Conference on Solanaceae Genomics (SOL), Nov 2011, Kobe, Japan. 265 p., ⟨10.1007/978-3-662-48535-4_12⟩
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absent
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titre
Disease resistance in melon and its modification by molecular breeding techniques
auteur
Michel Pitrat
article
8. Joint Conference on Solanaceae Genomics (SOL), Nov 2011, Kobe, France. ⟨10.1007/978-3-662-48535-4⟩
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absent
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titre
Phenotypic and genetic trends in the evolution under domestication of Solanaceae
auteur
M-Christin Daunay, Christopher Sauvage, Mathilde M. Causse, Alain Palloix, Jaime Prohens
article
8. Solanaceae Genome Joint conference; 2. Cucurbitaceae Genome Joint conference, Nov 2011, Kobe, Japan
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titre
Phenotypic and genetic trends in the evolution under domestication of melon
auteur
Michel Pitrat
article
8. Solanaceae Genome Joint conference; 2. Cucurbitaceae Genome Joint conference, Nov 2011, Kobe, Japan
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titre
Sensibilités variétales. Pêchers & Abricotier. Bilan de 14 années d’observation
auteur
Claude Eric Parveaud, Christelle Gomez, Gilles Libourel, François Warlop, Laurent Brun, Vincent Mercier, Armand Guillermin, Guy Clauzel, Jean Marc Audergon
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2011, Avignon, France. 19 p
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Conference papers
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https://hal.inrae.fr/hal-02805555/file/Sensibilite%20abricot%20peche%20MIFFEL%202011%20Parveaud%20et%20al_1.pdf BibTex
titre
Genetic and genomic tools in pepper to decipher disease resistance traits: Progress and prospects
auteur
Véronique Lefebvre
article
Chinese-French Workshop on Pepper Genetics and Breeding, Oct 2011, Bejing, China
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titre
Combination of field margin manipulation and genetic resistance to limit Aphis gossypii outbreaks and virus epidemics in melon crops
auteur
Alexandra Schoeny, Patrick Gognalons, Nathalie Boissot, Pascale Mistral, Virginie Chareyron, Catherine Wipf-Scheibel, Hervé Lecoq
article
Towards a multi-scale approach for improving pest management. System management for sustainable insect pest control: from local to regional approaches, Oct 2011, Montpellier, France
resume
Aphis gossypii is a major pest of Cucurbitaceae crops as much by causing primary damages (sap uptake) as by being an important vector of viruses. The Vat gene confers to the melon a resistance to the colonization by A. gossypii as well as a resistance to the not persistent viruses (CMV, WMV,..) transmitted by these aphids. It is however ineffective to block the transmission of the viruses carried by other aphid species. The use of Vat is thus generally coupled with aphicid treatments to limit the viral transmission by the not colonizing aphids. However, the progressive reduction of the usage of the phytosanitary products in the crop protection imposed by the evolution of the regulation (plan Ecophyto 2018) leads to look for new strategies integrating cultural practices and genetics for the management of the bioagressors. The bibliography suggests that the management of field margins (strips sown with non host grass or flower mixtures) could be an effective option to decrease 1) the aphid pressure (and thus the risk of bypassing Vat) by favoring the development of natural enemies and 2) the virus pressure by constituting a filter reducing the viral load of aphids before they reach the crop. The hypothesis tested in this project is that an adequate manipulation of the environment of the crop can contribute to regulate the populations of aphids and\or their viral load and therefore to reduce the risk of viral epidemics. The effect of three types of field margins (naked soil, grassy strips and flower strips) is evaluated on the efficiency of the Vat-mediated resistance.
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Conference papers
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https://hal.inrae.fr/hal-02744706/file/43994_20111011083541165_1.pdf BibTex
titre
Exploitation of knowledge on Oomycete effectors to drive the discovery of durable late blight resistance in tomato
auteur
Véronique Lefebvre
article
4. Effectome Meeting, Sep 2011, Lauret, France
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titre
Genetics and genomics of disease resistance in pepper: focus on Phytophthora threats
auteur
Véronique Lefebvre
article
international symposium commemorating the 20th anniversary of Korea Capsicum Research Cooperative, Sep 2011, South Korea
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Conference papers
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https://hal.inrae.fr/hal-02744884/file/Lefebvre%202011%20Symposium%20Korea%20205-223_1.pdf BibTex
titre
La conservation et la connaissance des ressources génétiques d'espèces végétales cultivées: un enjeu pour l'agriculture et un défi pour les acteurs
auteur
Emmanuel E. Geoffriau, Michel Pitrat, Pascal Coquin, François Balfourier, M-Christin Brand-Daunay, Florence Esnault, Chrystelle Jouy, Jean-Eric Chauvin, Alain Label-Richardson, Francois Boulineau, Helene Burck, Laurence Feugey, Brigitte Maisonneuve, Emilie Balsemin, Claudie Lambert, Anne-Marie Sage-Palloix, Teresa Barreneche, Catherine Delaitre, Stéphane Fourtier, Anne Zanetto, Agnès Grapin, Jean-Marie Prosperi, Audrey Didier
article
Les ressources génétiques face aux nouveaux enjeux environnementaux économiques et sociétaux, Sep 2011, Montpellier, France
resume
absent
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Conference papers
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https://hal.science/hal-01189505/file/43650_20111213111414976_1.pdf https://hal.science/hal-01189505/file/43650_20120106122142128_2.PDF BibTex
titre
Metabolite profiling and feeding bioassays suggest a major role for a dicaffeoylquinic acid in induced resistance of peach to Myzus persicae aphid
auteur
Jean-Luc Poëssel, Marie-Hélène Sauge, Michael Staudt, Claire Dufour, Catherine Deborde, Yves Rahbé, B. Jackson, Cédric Renaud, Mickael M. Maucourt, Marie-Noëlle Corre, H. El-Aouni, Jean Philippe Lacroze, Annick Moing
article
PRIR 2011 Joint International Workshop: "PR-proteins Workshop" and the "Working Group Induced Resistance in Plants Against Insects and Diseases", Sep 2011, Neuchâtel, Switzerland
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titre
Signatures de sélection et évolution d'effecteurs candidats dans des populations pathogènes soumises aux résistances de l'hôte
auteur
Romain R. Valade, Aurélie A. Ducasse, Jean-Baptiste J.-B. Genty, Brigitte B. Maisonneuve, R. R. Michelmore, Claire C. Neema
article
Petit Pois Déridé 2011; 33. Réunion annuelle du Groupe d'Etude de Biologie et Génétique des Populations, Aug 2011, Toulouse, France. 1 p
resume
absent
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Conference papers
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https://hal.science/hal-00999904/file/48349_20120210012231551_1.pdf BibTex
titre
Virulence of Bremia lactucae populations in Southern France between 2006 and 2011
auteur
Brigitte B. Maisonneuve, Sandrine Jeuniaux, Emilie Juillard, Marion M. Lovera
article
Eucarpia Leafy Vegetables 2011, Aug 2011, Lille, France
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https://hal.inrae.fr/hal-02745037/file/51518_20120210103754638_1.pdf BibTex
titre
Current diseases and pests in lettuce in Western Europe
auteur
Brigitte B. Maisonneuve, Dominique Blancard
article
Eucarpia Leafy Vegetables 2011, Aug 2011, Lille, France
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Conference papers
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https://hal.inrae.fr/hal-02745687/file/51050_20120210100304137_1.pdf BibTex
titre
Population structure of Bremia lactucae isolated from lettuce culture in France
auteur
Romain R. Valade, Claire C. Neema, Brigitte B. Maisonneuve
article
Eucarpia leafy Vegetables 2011, Aug 2011, Lille, France. pp.33
resume
absent
typdoc
Conference papers
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https://hal.science/hal-00999915/file/49390_20120210101109176_1.pdf BibTex
titre
Impact of selection pressure exerted by host resistance gene on population genetic structure of Bremia lactucae
auteur
Romain Valade, Aurélie Ducasse, Brigitte Maisonneuve, Claire Neema
article
13. congress of the European Society for Evolutionnary Biology, Aug 2011, Tübingen, Germany. 1 p
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absent
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titre
MDHAR links the ascorbate-glutathione cycle to carbohydrate metabolism and sink organ development in tomato
auteur
Noé Gest, Cecile Garchery, Rebecca Stevens
article
Annual Meeting of the American Society of Plant Biologists, Aug 2011, Minneapolis, United States
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titre
Ascorbate redox control of plant growth, carbon metabolism and source-sink regulation in tomato
auteur
Noé Gest, Cecile Garchery, Mathilde M. Causse, Hélène Gautier, T Do Phuc, Allistair R. Fernie, Ana Jimenez, Pierre Baldet, Christophe Rothan, Rebecca Stevens
article
10. International Conference on reactive Oxygen and Nitrogene species in Plants, Jul 2011, Budapest, Hungary
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Conference papers
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https://hal.inrae.fr/hal-02747094/file/49885_20120106025601491_1.pdf BibTex
titre
Breeding for disease resistance in plants, moving from efficiency to durability
auteur
Alain Palloix
article
4. Plant Protection Congress of Turkey, Jun 2011, Kahramanmaras, Turkey
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Conference papers
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https://hal.inrae.fr/hal-02744503/file/Palloix%202011%20NPP-Turkey_1.pdf https://hal.inrae.fr/hal-02744503/file/Palloix%202011%20NPP-Turkey_2.doc BibTex
titre
Infrared spectroscopy: a rapid tool for apricot characterization
auteur
Sylvie Bureau, Marielle Boge, Catherine M.G.C. Renard, Jean Marc Audergon
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
resume
The objective of this work, using the infrared spectroscopy applied to predict some apricot fruit quality traits, was to test on fruit harvested in 2010 the robustness of models established in 2005 (Bureau et al., 2009a,b). Two wavelength regions have been tested for apricot fruit quality analysis, the near-infrared (NIR, 800-2500 nm) applied on intact fruits and the mid-infrared (MIR, 4000-650 cm(-1)) on fruit slurries. Results of prediction were acceptable for SSC in NIR and for SSC and TA in MIR independently of the year. A methodology was then proposed for each year with i) a selection of a representative set of samples, ii) the development of prediction models using both spectral and classical measurements, and iii) the use of these models to predict quality traits in all other samples of the same year.
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titre
Influence of temperature on apricot fruit respiration and ethylene production
auteur
Barbara Gouble, Patrice Reling, Catherine M.G.C. Renard, Jean Marc Audergon
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
resume
To define optimal permeability of a composite wheat gluten-paper material for a new packaging film, elaborated in the Tailorpack project, physiological parameters modelling was conducted on two contrasted apricot cultivars ('Ravicille' and 'Bergeron'). Effects of temperature on respiration and ethylene production have been monitored and Q(10) were estimated at picking time and during postharvest storage. 'Ravicille' presented higher respiration and ethylene production rates, at all analysed temperatures, than 'Bergeron', while Q(10) values were more or less similar. For both cultivars, Q(10) increased with storage time. This result, added to the variability between cultivars, will have major impact on film conception.
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titre
Inheritance of phenological traits in apricot progenies
auteur
Jean Marc Audergon, David Ruiz, J.A. Campoy, J.A. Salazar, Stefano Tartarini, P. Martínez-Gómez, J. Egea, Patrick Lambert, Alain Blanc, Guillaume Roch, Guy Clauzel, L. Dondini
article
15. International Symposium, Jun 2011, Yerevan, Armenia
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Conference papers
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https://hal.inrae.fr/hal-02747058/file/40268_20111031092439465_1.pdf BibTex
titre
Apricot core collection assessment using morphological characterization
auteur
Lamia Krichen, Hedia Bourguiba, Jean Marc Audergon, Neïla Trifi-Farah
article
15. International Symposium, Jun 2011, Yerevan, Armenia
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Conference papers
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https://hal.inrae.fr/hal-02747317/file/47659_20111031111036415_1.pdf BibTex
titre
Apricot diffusion in the mediterranean basin : a serious loss of genetic diversity
auteur
Hedia Bourguiba, Lamia Krichen, Neïla Trifi-Farah, Bouchaib Khadari, Ali Mamouni, Samia Trabelsi, Claudio d'Onofrio, José Egea-Caballero, Bayram-Murat Asma, Jean Marc Audergon
article
15. International Symposium, Jun 2011, Yerevan, Armenia
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Conference papers
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https://hal.inrae.fr/hal-02749357/file/47861_20111031113806408_1.pdf BibTex
titre
Results of French apricot rootstock trials
auteur
Henri Duval, Martin Masse, Michel Jay, Jean Lichou
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
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Conference papers
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https://hal.inrae.fr/hal-02746087/file/40311_20110908024041711_1.pdf BibTex
titre
Performance of a model to predict the flowering date of apricot in three different regions of South France
auteur
Lucia Andreini, Patrick P. Bertuzzi, Jean Marc Audergon, Sylvain Satger, Marie Elise Liennard, Raffaella Viti
article
15. International Symposium, Jun 2011, Yerevan, Armenia
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Conference papers
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https://hal.inrae.fr/hal-02749445/file/43331_20110912094142240_1.pdf BibTex
titre
Suceptibility to Sharka (Plum pox virus) of several Prunus rootstocks against Dideron and Marcus strains
auteur
Manuel Rubio, Federico Dicenta, Martin Masse, Henri Duval
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
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Conference papers
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https://hal.inrae.fr/hal-02747149/file/45104_20110908030221119_1.pdf BibTex
titre
Inheritance of phenological traits in apricot progenies
auteur
Jean Marc Audergon, Guy Clauzel, Alain Blanc, Guillaume Roch, Patrick Lambert, D. Ruiz, J. A. Campoy, J. A. Salazar, P. Martínez-Gómez, J. Egea, Sylvie Bureau, Barbara Gouble, Marielle Boge, Patrice Reling, Catherine M.G.C. Renard, L. Dondini, S. Tartarini
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
resume
The impact of global changes is becoming a serious concern at a world scale, and its influence could be rather large on perennial plant behaviour. Within fruit species, advances in blooming dates and floral disorders in relation to temperature increase during period of dormancy and bud burst have already been shown. Unfortunately among fruit species, apricot is expected to be one of the most susceptible in relation to the restricted adaptation of the local cultivars to narrow geographic areas. Thus, in order to overcome the negative trends and to safeguard apricot fruit production, the identification of the genetic components involved in blooming and maturity periods becomes of major interest. A global genetic analysis has been conducted on 4 apricot progenies issued from different genetic backgrounds; two progenies were cultivated in Avignon (Fr), one in Bologna (It), and one in Murcia (Sp). The four progenies were phenotyped for blooming and maturity dates at least for 2 years (except the Italian one evaluated only for the maturity date). The segregations have been examined and QTL analyses have been performed on the whole data set. The QTL stability across years has been analysed for the two traits. The influence of the genetic background has been discussed.
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titre
Temperature effect on respiration and ethylene production of two apricot varieties
auteur
Barbara Gouble, Patrice Reling, Jean Marc Audergon, Catherine M.G.C. Renard
article
15. International Symposium, Jun 2011, Yerevan, Armenia
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Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02745887/file/40073_20110912091210918_1.pdf BibTex
titre
Inheritance of fruit quality traits in apricot progenies
auteur
D Ruiz, L. Dondini, J.A. Salazar, Stefano Tartarini, M.D. Nortes, P. Martínez-Gómez, J. Egea, Guy Clauzel, Patrick Lambert, Alain Blanc, Sylvie Bureau, Maryse Reich, Patrice Reling, Barbara Gouble, Catherine M.G.C. Renard, Jean Marc Audergon
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
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https://hal.inrae.fr/hal-02749079/file/46585_20110908112901220_1.pdf BibTex
titre
An integrated apricot breeding program in France joining CEP Innovation - Centrex and INRA
auteur
Jean Marc Audergon, Alain Blanc, Frederic Gilles, Guy Clauzel, J.M. Broquaire, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, C. Pitiot
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia
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https://hal.inrae.fr/hal-02749046/file/47306_20110908102623142_1.pdf BibTex
titre
A rapid tools for physiological study of apricot fruit
auteur
Sylvie Bureau, Barbara Gouble, Jean Marc Audergon, Catherine M.G.C. Renard
article
15. International Symposium, Jun 2011, Yerevan, Armenia
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02745371/file/42568_20111031100213921_1.pdf BibTex
titre
Heuristic value of a ‘Virtual fruit’ model of peach fruit quality and sensitivity to brown rot
auteur
Michel M. Génard, Bénédicte Quilot-Turion, Mohamed Ahmed Ould Sidi, Abdeslam Kadrani, Nadine Hilgert, Francoise F. Lescourret
article
9. International Symposium on modelling in Fruit Research and Orchard management, Jun 2011, Saint-Jean-sur-Richelieu, Canada. 7 p
resume
This work aimed at investigating the heuristic potential of a process-based model of peach fruit expressing seasonal changes in several quality attributes of fruit (e.g., size, percentages of flesh, water, sugar and acid contents) and fruit sensitivity to brown rot (skin density of cracks). Firstly, we showed that this “Virtual Fruit” could be used to analyze the impact of a single mutation which decreased the fruit’s requirement for carbon on peach fruit behavior. The mutation triggered large effects on several variables of the fruit development model (growth, respiration and metabolism) and delayed the fruit developmental rate. Such a virtual approach could lead to new ways of exploring the impact of mutations, or naturally occurring genetic variations, in silico, under different environmental conditions. Secondly, we illustrated how the “Virtual Fruit” could be possibly used to design peach ideotypes with high fruit mass and sweetness and low skin density of cracks. Since these traits are antagonists, we treated this design as a multi-objective optimization problem. An evolutionary algorithm was applied to solve this multi-objective optimization problem based on the “Virtual Fruit”. The optimized variables were six genetic parameters of the “Virtual Fruit”. This optimization procedure provided a large diversity of solutions (ideotypes). Ideotypes with low fruit mass had a high sweetness and low skin density of cracks. In a current breeding scheme, fruit mass would be the only criterion, but alternative schemes could be considered for the future, favoring organoleptic quality or environment friendly practices, or more generally, aiming at finding acceptable trade-offs between several criteria. In this case, modelling and model-based optimization would be helpful.
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titre
Particle swarm optimization for designing ideotypes for sustainable production systems
auteur
Abdeslam Kadrani, Mohamed Mahmoud Ould Sidi, Bénédicte Quilot-Turion, Michel M. Génard, Francoise F. Lescourret
article
ICSI 2011: International conference on swarm intelligence, Jun 2011, Cergy, France. 3 p
resume
Agricultural systems have to adapt, in a changing climatic context, to face the growing social demand in terms of organoleptic, nutritional and environmental quality of food products. Thus, a crucial question for the future is how to manage fruit quality by finding the best combinations of genetic resources and cultural practices adapted to, and respectful of specific environments. This question was addressed using a particle swarm optimization and a process-based model. The obtained results show the interest of such as approach.
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Inheritance of fruit quality traits in apricot progenies
auteur
D. Ruiz, Luca Dondini, J.A. Salazar, Stefano Tartarini, M.D. Nortes, Pedro Martínez-Gómez, J. Egea, Guy Clauzel, Patrick Lambert, A. Blanc, Sylvie Bureau, M. Reich, Patrice Reling, Barbara Gouble, Catherine C. Renard, Jean Marc Audergon
article
15 th International Symposium on Apricot Breeding and Culture , Jun 2011, Yerevan, Armenia
resume
The knowledge on the transmission of fruit quality traits has a great importance in order to optimize the inter-cultivar crosses design and to improve the efficiency of apricot breeding programmes. Four apricot progenies issued from different genetic origins were evaluated during two years; two apricot progenies cultivated in Avignon (France), one in Bologna (Italy), and other one in Murcia (Spain). Fruit quality characterization was based on physical measurements (fruit weight, colour, and firmness) and biochemical measurements (soluble solids content, and titratable acidity). Phenotypic datasets showed distribution of seedlings with regard to the parents, with a high variability for all analyzed quality parameters. An inter-annual variation was observed for the mean values of all the genotypes between the two years of the study, indicating the influence of the environmental conditions on fruit characteristics. Results indicated a quantitative inheritance of all quality traits in the apricot progenies indicating their polygenic nature. In general we observed in most cases a normal distribution of quality traits in the observed populations. However, in contrast to intermediate expected values between the parents, a large number of seedlings showed lower or higher values than their parents for many quality parameters. In this sense, results evidenced an extra genetic influence of the parents (the whole genetic background) on the transmission of fruit quality traits in apricot, which should be taken into consideration with inter-cultivar crosses design.
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Replication of inaccurate viruses and transmissible genetic diversity: crosstalk between modelling and high-throughput sequencing
auteur
Gael Thébaud, Marco Morelli, Sylvie Dallot, Joel J. Chadoeuf, Gerard Labonne, Daniel Haydon
article
4. Plant Virus Ecology Network Workshop (PVEN), May 2011, Montpellier, France
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Bien choisir les espèces et les variétés
auteur
M-Christin Daunay, Richard Brand, Dominique Blancard
article
13. Colloque Scientifique, May 2011, Montpellier, France
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https://hal.inrae.fr/hal-02749852/file/46786_20111109025649439_1.pdf BibTex
titre
Genome admixture of Solanum lycopersicum var. cerasiforme allows successful association mapping of quality-traits and adds insights in tomato domestication
auteur
Mathilde M. Causse, Nicolas N. Ranc, Stephane Munos, Dominique D. Brunel
article
Eucarpia Tomato 2011, 17. Eucarpia Meeting Section Vegetables Working Group Tomato, Apr 2011, Malaga, Spain. 1 p
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titre
Application des algorithmes génétiques pour la conception de systèmes de culture durable
auteur
Mohamed Mahmoud Ould Sidi, Bénédicte Quilot-Turion, Abdeslam Kadrani, Nadine Hilgert, Michel M. Génard, Francoise F. Lescourret
article
12. Congrès annuel de la société française de la recherche opérationnelle et de l'aide à la décision., Mar 2011, Saint-Etienne, France. 2 p
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https://hal.inrae.fr/hal-02809734/file/43547_20110728021149244_1.pdf BibTex
titre
Evolution des souches de Bremia. Durabilité des méthodes de protection
auteur
Brigitte B. Maisonneuve
article
Rencontres phytosanitaires CTIL/SDQPV légumes et fraise, Feb 2011, Plan d'Orgon, France. pp.1-13
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https://hal.inrae.fr/hal-02808721/file/50628_20120217085355550_1.pdf BibTex
titre
Vers des cultures fruitières et légumières à hautes performances environnementales
auteur
Benoit Jeannequin, Francoise Dosba, Daniel Plénet, Michel Pitrat, Jean-Eric Chauvin
article
Salon International de l'Agriculture, Feb 2011, Paris, France. pp.73-85
resume
In France, fruit and vegetable productions contribute 2% of the UAA and are characterised by a diversity of cultivated species and cropping systems with contrasting environmental impacts. Facing new stakes of implementing horticulture with high environment performance, Inra carries out researches targeting a reduction of the environmental impact of these crops and conc eption of innovative production systems. The main results achieved during the recent years concern optimisation of the management climatic conditions in greenhouses, optimised management of irrigation and fertilisation, creation of varieties with multiple resistance as well as durable management of resistances to pests and diseases, conception of production systems leading to reduced phytosanitary risks. Beyond researches in organic farming, the implementation of integrated pest management in fruit production and, more recently, integrated biological control in greenhouses are main contributions to environment preservation. Present approaches of Inra are more global and target low input production systems with high environmental and economic performances. They require modelling of complex systems and multiple partnerships. To feed and study these questions, Inra reinforces its collaborative structures, especially through Group of Scientific Interest (GIS) such as GIS PICLeg and, soon, GIS Fruits
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https://hal.science/hal-01512047/file/Jeannequin_2011_1.pdf BibTex
titre
Génétique d'association pour la qualité chez la tomate
auteur
Mathilde M. Causse, Nicolas N. Ranc, Stephane Munos, Dominique D. Brunel
article
Assemblée Générale de l'ASF : Génétique d’association et Amélioration des Plantes, Evolution des outils de génotypage et de phénotypage à haut débit, Feb 2011, Versailles, France. 9 p
resume
Chez la tomate, l’amélioration pour la qualité du fruit est rendue difficile par la multiplicité et la complexité des caractères. La cartographie de QTL a permis la caractérisation génétique de ces caractères. L’objectif est maintenant d’identifier les gènes sous-jacents aux QTL. Nous avons utilisé la cartographie par déséquilibre de liaison (DL) dans ce but. Pour éviter les fausses associations entre les caractères et les polymorphismes moléculaires, la structure génétique a été prise en compte dans l’analyse. La tomate cultivée montre un faible niveau de diversité génétique, ce qui réduit la résolution de cartographie. Le génome des tomates de type cerise (S. lycopersicum var. cerasiforme) est une mosaïque entre celui de la tomate cultivée et celui de l’ancêtre sauvage. Ce mélange devrait augmenter la résolution des études d’association. Nous avons utilisé une « core collection » focalisée sur des accessions de type cerise pour réaliser une étude d’association, focalisée sur le chromosome 2. Nous avons analysé l’étendue du DL en fonction de la distance génétique et physique. Des associations entre des polymorphismes et les phénotypes étudiés ont été détectés avec des méthodes prenant en compte la structure génétique. Nous avons montré l’intérêt d’utiliser la structure en mosaïque du génome des accessions de type cerise pour surmonter les limitations de résolution dans les analyses d’associations chez une espèce cultivée autogame. Nous avons validé des QTL identifiés précédemment et nous avons trouvé des associations avec de nouveaux QTL et de nouveaux gènes candidats.
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Mejoramiento genético en el melocotonero: novedades y perspectivas para la resistencia a los bioagresores y la calidad del fruto
auteur
Thierry Pascal, Patrick Lambert, Bénédicte Quilot-Turion, Véronique Decroocq, Marie-Helene Sauge-Collet
article
Jornada de Especializacion - Innovacion Varietal en Melocoton, Nectarina y Paragueyo: Actualizacion, Cogullada, Feb 2011, Zaragoza, España
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Towards the identification of new host factors required for plant susceptibility to potyviruses: novel routes to resistance
auteur
Laurence Ouibrahim, André Moretti, Aurélie Salgues, Ana Giner Rubio, Hervé Lecoq, Christophe Robaglia, Carole Caranta
article
13. Rencontres de Virologie Végétale, Jan 2011, Aussois, France
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https://hal.inrae.fr/hal-02747103/file/50751_20120402092050925_1.pdf BibTex
titre
Genome-wide association genetics to identify loci involved in responses to Potyviruses in plants
auteur
Valerie Schurdi-Levraud, Zofia Nehr, Patrick Cosson, Carole Caranta, F. Roux, Frederic Revers
article
13. Rencontres de Virologie Végétale, Jan 2011, Aussois, France. p. 82
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https://hal.inrae.fr/hal-02807052/file/50374_20120402090450412_1.pdf BibTex
titre
Réplication des virus "infidèles" et diversité génétique transmissible : apports croisés de la modélisation et du séquençage à haut débit
auteur
Gael Thébaud, Marco Morelli, Sylvie Dallot, Joel J. Chadoeuf, Gerard Labonne, Daniel Haydon
article
13. Rencontres de Virologie Végétale (RVV 2011), Societe Francaise de Phytopathologie (SFP). FRA. Institut National de la Recherche Agronomique (INRA).; Agropolis International. Montpellier, FRA., Jan 2011, Aussois, France
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Des régions clé de la protéine pvr2-eIF4E1 du piment gouvernent la résistance au potyvirus et son spectre
auteur
Séverine Lacombe, Sophie Ewert, André Moretti, Claire Fouet, Carole Caranta
article
13. Rencontres de Virologie Végétale, Jan 2011, Aussois, France. p. 27
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https://hal.inrae.fr/hal-02811302/file/50322_20120402090926901_1.pdf BibTex
titre
Pourquoi une résistance polygénique est-elle plus durable qu’une résistance monogénique ?
auteur
Julie Quenouille, Josselin Montarry, Alain Palloix, Benoît Moury
article
13. Rencontres de Virologie Végétale, Jan 2011, Aussois, France
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https://hal.inrae.fr/hal-02750189/file/47732_20110131102352846_1.pdf BibTex
titre
Estimating risk factors for virus dissemination from incomplete epidemiological surveillance data
auteur
David Pleydell, Gael Thébaud, Samuel S. Soubeyrand, Sylvie Dallot, Sylvain Grizard, Joel J. Chadoeuf, Gerard Labonne
article
13. Rencontres de Virologie Végétale (RVV 2011), Jan 2011, Aussois, France
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Analyse et enjeux de la valeur agronomique technologique et environnementale chez les espèces fruitières
auteur
Gilbert Grivault, Richard Brand, Laurent Brun, Charles Eric Durel, Daniel Plenet, François Warlop, Jean Marc Audergon
article
6. Rencontres du Végétal, Jan 2011, Angers, France
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https://hal.inrae.fr/hal-02750112/file/42626_20110222085517852_1.pdf BibTex
titre
Le Groupe « Espèces Fruitières » du Département Génétique et Amélioration des Plantes de l'INRA, un partenaire au service de l’environnement et de l’innovation pour les territoires à vocation fruitière, les producteurs et les consommateurs
auteur
Jean Marc Audergon, Teresa Barreneche, Mathilde M. Causse, Elisabeth E. Chevreau, Evelyne E. Costes, Beatrice Denoyes-Rothan, Olivier Pailly, Yves Lespinasse
article
6. Rencontres du Végétal, Jan 2011, Angers, France
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https://hal.inrae.fr/hal-02750402/file/41794_20110222093648304_1.pdf BibTex
titre
Temperature effect on respiration and ethylene production of two apricot varieties.
auteur
Barbara Gouble, Patrice Reling, Jean Marc Audergon, Catherine C. Renard
article
15 th International Symposium on Apricot Breeding and Culture , 2011, Yerevan, Armenia
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An integrated apricot breeding program in France joining CEP Innovation - Centrex and INRA.
auteur
Jean Marc Audergon, Alain Blanc, Gilles Fréderic, Guy Clauzel, M. Broquaire, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, C. Pitiot
article
International Symposium on Apricot Breeding and Culture, 2011, Yerevan, Armenia
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Other publications

titre
La conservation et la connaissance des ressources génétiques d'espèces végétales cultivées : un enjeu pour l'agriculture et un défi pour les acteurs
auteur
Emmanuel E. Geoffriau, Michel Pitrat, Pascal Coquin, François Balfourier, Marie- Christine Daunay, Florence Esnault, Chrystelle Jouy, Jean-Eric Chauvin, Alain Label-Richardson, François Boulineau, Helène Burck, Laurence Feugey, Brigitte Maisonneuve, Emilie Balsemin, Claudie Lambert, Anne-Marie Sage-Palloix, Teresa Barreneche, Catherine Delaitre, Stéphane Fourtier, Anne Zanetto, Agnès Grapin, Jean-Marie Prosperi, Audrey Didier
article
2011, non paginé
resume
La conservation et la connaissance des ressources génétiques d'espèces végétales cultivées : un enjeu pour l'agriculture et un défi pour les acteurs
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Poster communications

titre
Metabolite profiling and feeding bioassays suggest a major role for a dicaffeoylquinic acid in induced resistance of peach to Myzus persicae aphid
auteur
Jean-Luc Poessel, Marie-Hélène Sauge, Michael Staudt, Claire Dufour, Catherine Deborde, Yvan Rahbé, B. Jackson, Cédric Renaud, Mickael M. Maucourt, Marie Noëlle Corre, H. El-Aouni, Jean Philippe Lacroze, Annick Moing
article
PRIR 2011 Joint meeting of the "PR-proteins Workshop" and the "Working Group Induced Resistance in Plants Against Insects and Diseases", Sep 2011, Neuchatel, Switzerland. 154 p., 2011, PR-Proteins and Induced Resistance Against Pathogens and Insects. Abstracts book
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https://hal.inrae.fr/hal-02748156/file/41817_20111027014430108_1.pdf BibTex
titre
Improvement of the differential lettuce set for Bremia virulence evaluation: new sativa monogenic lines
auteur
Brigitte B. Maisonneuve
article
Eucarpia Leafy Vegetables 2011, Aug 2011, Lille, France. 2011, Proceedings Eucarpia Leafy Vegetables 2011
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https://hal.inrae.fr/hal-02747007/file/51609_20120210104128452_1.pdf BibTex
titre
Développement de biopesticides d’origine végétale pour l’horticulture : méthodes de production, spectre d’action et validation in vivo de l’efficacité des acides dicaféoylquiniques
auteur
Jean-Luc Poessel, Camille Croset, Marie Noëlle Corre, Elodie Lecerf, Jawad Aarrouf, Marie-Hélène Sauge, Stéphanie Servier, Jean Philippe Lacroze, Fanny Calmels, Claire Dufour, Michele Loonis, Philippe C. Nicot, Marc Bardin, Claire Troulet, Catherine Deborde, Vincent Lebot, Antoine Champagne, Gérard Febvay, Yvan Rahbé, Jean-Luc Verdeil
article
ANR - Grand colloque Biologie et Santé 2011, Jul 2011, Lyon, France. 1 p., 2011
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titre
An Integrated Apricot Breeding Program in France Joining CEP Innovation - CENTREX and INRA
auteur
Jean Marc Audergon, Alain Blanc, Frederic Gilles, Guy Clauzel, Jean Michel Broquaire, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, Guillaume Frémondière, Christelle Pitiot
article
15. International Symposium on Apricot Breeding and Culture, Jun 2011, Yerevan, Armenia. 2011
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https://hal.inrae.fr/hal-02746028/file/Erev11_Audergon_apricot%20breeding_poster_1.pdf BibTex
titre
Ectopic expression of translation initiation factor eIF4E extends the resistance spectrum against Lettuce mosaic virus in lettuce
auteur
Marianne Mazier, Raquel Sanjuan, Fabrice Flamain, Verane Sarnette, Emmanuel Botton, Sylvie S. German-Retana, Mathieu Cammas, André Moretti, Brigitte B. Maisonneuve, Carole Caranta
article
13. Rencontres de virologie végétale, Jan 2011, Aussois, France. p. 70, 2011
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https://hal.inrae.fr/hal-02746846/file/51777_20120330092446079_1.pdf BibTex

Reports

titre
Rapport final du projet : HortiBiope. Développement de biopesticides d'origine végétale pour l'horticulture: méthodes de production, spectre d'action et validation in vivo de l'efficacité des acides dicaféoylquiniques
auteur
Jean-Luc Poessel, Marie-Hélène Sauge, Claire Dufour, Philippe C. Nicot
article
[Contrat] 2011
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titre
Molecular basis of virus resistance mediated by host factors required for the infectious cycle
auteur
Carole Caranta
article
[Contrat] 2011
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https://hal.inrae.fr/hal-02807142/file/Compte-rendu%20final_MOVIe_%202011_1.pdf BibTex
titre
Influence de la diversité génétique du virus et de l’hôte sur le contournement d’une résistance au virus Y de la pomme de terre chez le piment
auteur
Ludovic Saint-Félix
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2011, 48 p
resume
Les résistances génétiques permettent une lutte efficace contre les maladies des cultures mais sont limitées par les capacités de contournement des pathogènes. Chez le piment, le fond génétique peut améliorer la durabilité de la résistance au virus Y de la pomme de terre (PVY) due au gène majeur pvr23 tandis que chez le PVY, le gène CI accentue ses capacités de contournement via les substitutions dans le gène d’avirulence (VPg). Nous avons déterminé une corrélation entre durabilité et résistance quantitative contrôlée par le fond génétique chez des variétés de piment non apparentées, et montré que les fonds génétiques favorisant la durabilité de pvr23 sont répandus dans la diversité génétique. Parallèlement, chez le PVY, nous avons recherché les mutations du gène CI impliquées dans l’amélioration du contournement, par création de virus recombinants. Deux acides aminés du tiers C-terminal de la protéine pourraient expliquer ces propriétés. La résistance partielle, mesurée par la concentration virale, apparaît comme un critère a priori de sélection pour la durabilité
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https://hal.inrae.fr/hal-02811163/file/45375_20110620114946678_1.pdf BibTex
titre
Résistance génétique du melon à Aphis gossypii
auteur
Marion Chambrier
article
[Stage] Institut Universitaire de Technologie (IUT), Digne les Bains, FRA. 2011, 53 p
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titre
Functional confirmation of candidate genes for a broad-spectrum resistance QTL against Phytophthora in Solanaceae
auteur
Véronique Lefebvre
article
[Contract] Institut National de la Recherche Agronomique (INRA). 2011
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Nouvelles sources de résistance au puceron Aphis gossypii chez le melon
auteur
Nathalie Boissot
article
[Contrat] 2011
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titre
Recherche d’interactions entre la capside du Raspberry Bushy Dwarf Virus (RBDV) et les protéines eIF4G d’Arabidopsis
auteur
Aramata Marena
article
[Stage] Faculté des Sciences de Luminy. Université de la Méditerranée (Aix Marseille 2), Marseille, FRA. 2011, 25 p
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https://hal.inrae.fr/hal-02805234/file/45379_20110721032223919_1.pdf BibTex

2010

Conference papers

titre
Acides caféoylquiniques et résistance au Monilinia
auteur
Bénédicte Quilot-Turion, Jean-Luc Poessel
article
Réunion finale HortiBiope, Dec 2010, Avignon, France
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titre
Vers l'intégration de composantes génétiques dans les modèles en vue de la création d'idéotypes
auteur
Bénédicte Quilot-Turion
article
Journées Modélisation du Réseau Qualité du Département EA, Nov 2010, Chateauneuf de Gadagne, France
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titre
QTLs detection for phenological traits within the Rosaceae family.
auteur
Elisabeth Dirlewanger, José Quero-Garcia, Loick Le Dantec, Evelyne E. Costes, Beatrice Denoyes-Rothan, Amélia Gaston, Bénédicte Quilot-Turion, Patrick Lambert, Jean Marc Audergon, David Ruiz, Jean-Marc J.-M. Celton, L. Dandini, S. Tartarini, E. Illa, P. Arus
article
5. Rosaceae Genomic Conference RGC5, Agricultural Research Council (ARC). Cape-Town, ZAF., Nov 2010, Cate-Town, South Africa
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titre
The french peach breeding program (INRA): an integrative approach for pest/disease resistance and fruit quality
auteur
Patrick Lambert, Jean-Luc Poessel, Camille Croset, Marie Noëlle Corre, Bénédicte Quilot-Turion, Veronique Signoret, Véronique Decroocq, David Tricon, Marie-Hélène Sauge, Sylvie Bureau, Catherine M.G.C. Renard, Thierry Pascal
article
5. Rosacea Genomics Conference, Nov 2010, Stellenbosch, South Africa. 1p
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https://hal.inrae.fr/hal-02819259/file/47008_20110331113855581_1.pdf BibTex
titre
QTL detection for phenological traits within the rosaceae family
auteur
Elisabeth Dirlewanger, José Quero-Garcia, Loick Le Dantec, Patrick Lambert, David Ruiz, Jean-Marc J.-M. Celton, L. Dondini, E. Illa, Bénédicte Quilot-Turion, Jean Marc Audergon, Stefano Tartarini, Pere Arus, Evelyne E. Costes, Beatrice Denoyes-Rothan
article
5. International Rosaceae Genomics Conference, Nov 2010, Stellenbosch, South Africa. 1 p
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titre
Towards optimization of anther culture and genotypic response for haploid plant production in sweet pepper
auteur
Alain Palloix
article
2. International Conference on Applied Biotechnology. African City of Technology, Oct 2010, Khartoum, Sudan
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titre
Analysis of the genetic determinism of phenological traits on Punus fruit species ans its interaction with envirnmental and climatic conditions
auteur
Elisabeth Dirlewanger, José Quero-Garcia, Maria-Das-Dores Lafargue, Loick Le Dantec, Jacques Joly, Jean-Yves Chevrollier, Patrick Lambert, Bénédicte Quilot-Turion, Jean Marc Audergon, Luca Dondini, Stefano Tartarini, Eudald Illa, Pere Arus, Anton Ivancic, Philippe Letourmy
article
Interdisciplinary congress ACCAE "Adapting to climate change : agriculture and ecosystems", Oct 2010, Clermont-Ferrand, France. 2 p
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titre
Étude du mode d’action des acides dicaféoylquiniques, molécules naturelles aphicides
auteur
Stéphanie Servier, Marie-Hélène Sauge, Gérard Febvay, Marie Noëlle Corre, Camille Croset, Jean Philippe Lacroze, Yvan Rahbé, Jean-Luc Poessel
article
16. Colloque de Biologie de l’Insecte, Oct 2010, Lyon, France. 126 p
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titre
Étude du mode d’action des acides dicaféoylquiniques, molécules naturelles aphicides
auteur
Stéphanie Servier, Marie-Hélène Sauge, Gérard Febvay, Marie Noëlle Corre, Camille Croset, Jean Philippe Lacroze, Yvan Rahbé, Jean-Luc Poessel
article
16. Colloque de Biologie de l’Insecte, Oct 2010, Lyon, France. 1 p
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titre
Intelligent fluorosensor: a fast tool to determine fluorescence characteristics
auteur
Alain Palloix
article
10. Nordic Phytosynthesis Congress (NPC10), Oct 2010, Tartu, Estonia
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Projet ANR Systerra : VirAphid Comment utiliser les résistances des plantes pour gérer durablement l'évolution des virus et des pucerons ?
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Nathalie Boissot
article
Journées du réseau Biologie et Adaptation des Pucerons et Organismes Associés (BAPOA), Oct 2010, Lyon, France
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Next-generation sequencing as source of SNP and structural variants information to improve breeding programs in Populus nigra
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Stefania Giacomello, Giusi Zaina, Fabio Marroni, Sara Pinosio, Cristian del Fabbro, Simone Scalabrin, Federica Cattonaro, Frederique Bitton, Marie-Christine Le Paslier, Dominique D. Brunel, Stéphane Schlub, Catherine Bastien, Véronique Jorge, Jennifer Dewoody, Maurizio Sabatti, Wout Boerjan, Gail Taylor, Patricia P. Faivre-Rampant, Michele Morgante
article
5. International Poplar Symposium;IPS V, Sep 2010, Orvieto, Italy
resume
Progress in applying new methods to forest tree breeding has understandably been slower because of the difficulty to acquire genomic data in the major commercial species. The situation has improved rapidly through whole genome sequencing in Populus trichocarpa, and the construction of large EST databases in poplar. Nevertheless, the genomic tools to develop molecular markers for advanced breeding are still not adequate, in particular for the native European poplar species, P. nigra. In the framework of a joint resequencing effort undertaken by the EU funded projects Evoltree, Noveltree and EnergyPoplar, we aim at producing a sequence-marker map made of SNPs, short InDels and copy number variants covering the whole black poplar genome by means of next generation sequencing. First, by using the Illumina/Solexa technology we resequenced three different European individuals at a coverage > 20X in order to obtain both SNP and structural variants information and a draft sequence for the black poplar genome. Pilot analyses showed the feasibility of using P. trichocarpa genome sequence as reference for P. nigra, given that 75% of P. nigra reads were uniquely mapped on the P. trichocarpa sequence. We will present data on types and frequency of sequence variants at the whole genome level based on the sequences of the three individuals. Then, to maximize SNP discovery all over the genome, sequence reads obtained from 50 additional European clones, which have been resequenced at 2X coverage, will be aligned to the black poplar draft consensus sequence. Such SNP detection will be supported and confirmed by P. nigra sequences of several gene fragments, which were resequenced in different natural European populations by using the traditional Sanger method. The final SNPs data will be used for high throughput genotyping of an association population made of 1100 poplar clones established in two experimental sites, in order to monitor genetic variation during the selection process and develop Marker-Assisted-Selection and Gene-Assisted-Selection breeding programs.
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https://hal.inrae.fr/hal-02755743/file/IPS-V%20Book%20of%20Abstracts_1.pdf BibTex
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Quantitative resistance to Plum pox virus in Prunus davidiana P1908: a possible resource for durable resistance in peach
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Patrick Lambert, M. Rubio, Christophe Tuero, Alexandre Bachellez, Thierry Pascal
article
International symposium on Plum pox virus; SharCo research worshop, Sep 2010, Sofia, Bulgaria
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Analysis of the resistance to PPV in a F1 progeny derived from the apricot cultivars 'Goldrich' and 'Moniqui'. Identification of the QTLs involved
auteur
Patrick Lambert, Guillaume Roch, Aurore Graillat, Jean Leonetti, Eric Martin, Anne Marie Ferréol, Alain Blanc, Jean Marc Audergon
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International symposium on Plum pox virus; SharCo research workshop, Sep 2010, Sofia, Bulgaria
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Genomic organization of the resistance to Plum pox virus in distinct apricot progenies through a quantitative meta-analysis
auteur
Véronique Decroocq, Grégoire Marandel, J. Salava, A. Abbott, Jean Marc Audergon, Patrick Lambert, Thierry Candresse, M.L. Badenes
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International symposium on Plum pox virus 2010 - SharCo research workshop, Sep 2010, Sofia, Bulgaria
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Near isogenic lines of tomato for texture contrasted QTL showed altered pericarp histology and cell wall chemistry
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Marc Lahaye, Bernard Quemener, Marie Francoise M. F. Devaux, Mervin Poole, Mathilde M. Causse, Graham Seymour
article
7. Solanaceae Conference, Sep 2010, Dundee, United Kingdom
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“Genetical proteomics” to tomato fruit quality
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Mireille Faurobert, Rémy Aurand, Emadeldin Konozy, David Page, Jean-Paul Bouchet, Mathilde M. Causse
article
7. Solanaceae Conference, Sep 2010, Dundee, United Kingdom
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Genome admixture of Solanum lycopersicum var cerasiforme allows successful association mapping in tomato
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Nicolas N. Ranc, Stephane Munos, Marie-Christine Le Paslier, Aurelie A. Chauveau, Rémi Bounon, Sophie Rolland, Jean-Paul Bouchet, Dominique D. Brunel, Mathilde M. Causse
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7. Solanaceae Conference, Sep 2010, Dundee, United Kingdom
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La variabilité des gènes d’avirulence viraux conditionne t-elle la durabilité des résistances chez les plantes ?
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Benoît Moury, Bérenger Janzac, Frederic Fabre, Alain Palloix
article
Colloque national d'écologie scientifique, Sep 2010, Montpellier, France
resume
Afin de définir de nouveaux critères prédictifs de la durabilité des résistances des plantes à leurs bioagresseurs, nous avons testé l’existence d’un lien entre d’une part la durabilité des résistances aux virus et d’autre part la diversité moléculaire des facteurs d’avirulence correspondants ou bien la contrainte évolutive s’exerçant sur ces facteurs. En effet, l’émergence de populations virales capables de contourner une résistance nécessite l’accumulation d’une ou plusieurs mutations dans ces facteurs d’avirulence. Or, plus un facteur d’avirulence est variable, plus il aura de chances que sa séquence possède ou acquière les mutations particulières permettant au virus de s’adapter à la résistance correspondante et moins cette résistance sera durable. Par ailleurs, plus l’évolution du facteur d’avirulence est contrainte, plus les mutations qui pourraient apparaître dans ce gène auront des effets négatifs sur la fitness du virus et plus la résistance correspondante sera durable. Afin de tester ces hypothèses, nous avons retenu 20 pathosystèmes plante-virus pour lesquels il était possible d’estimer l’ensemble de ces paramètres. La contrainte évolutive exercée sur les facteurs d’avirulence a été estimée par le ratio entre les taux de substitutions non synonymes et synonymes affectant les gènes codant pour ces facteurs. Les résultats n’indiquent aucun lien significatif entre la diversité moléculaire des facteurs d’avirulence et la durabilité des résistances correspondantes. Par contre, un lien significatif a été observé entre la contrainte évolutive exercée sur les facteurs d’avirulence et la durabilité des résistances correspondantes. Nos résultats suggèrent également que l’estimation de cette contrainte évolutive pourrait être utilisée comme un prédicteur précoce de la durabilité potentielle des gènes de résistance, utilisable en amont dans les schémas de création variétale.
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Identification d’indicateurs de durabilité des résistances des plantes aux maladies
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Josselin Montarry, Bérenger Janzac, Alain Palloix, Benoît Moury
article
Colloque national d'écologie scientifique, Sep 2010, Montpellier, France
resume
Une résistance est dite durable si elle a conservé son efficacité après avoir été utilisée de manière prolongée sur de grandes surfaces et en présence de l’agent pathogène. Cette définition ne permet pas de prédire la durabilité des résistances, et pour identifier des indicateurs de durabilité, il est nécessaire de comprendre les modalités d’adaptations des populations pathogènes. L’interaction entre le piment et le virus Y de la pomme de terre constitue un système d’étude pertinent pour rechercher des critères de durabilité, entre autre parce que les résistances disponibles présentent des durabilités contrastées. Nous cherchons ici à expliquer la forte durabilité de la résistance dominante qui est conférée au piment par le gène Pvr4. En effet, ce gène de résistance est déployé sur le terrain depuis une vingtaine d’années sans qu’aucune adaptation du virus ciblé n’ait été observée. Pour ce faire, nous nous sommes intéressés au déterminisme génétique de l’adaptation du virus aux génotypes de piment porteurs de Pvr4, ce qui consiste à identifier la ou les mutations responsable(s) de cette adaptation (mutations de virulence), mais aussi à l’impact de ces mutations sur la fitness du virus, en mesurant des « coûts de virulence » et d’éventuelles compensations de ces coûts. Une unique substitution nucléotidique dans la région codant la polymérase virale suffit pour l’adaptation. Il semble donc que, dans le cas de Pvr4, le nombre de mutations conférant la virulence n’explique pas la forte durabilité observée. Les mesures de la fitness relative des variants virulents et avirulents dans des plantes sensibles inoculées par les deux virus en mélange montrent que l’adaptation à Pvr4 confère au PVY un fort coût de compétitivité. A plus long terme, aucune mutation compensatrice n’a été trouvée, et la réversion mutationnelle semble être la seule solution pour le variant virulent de regagner de la fitness. Le fort coût de virulence explique donc en partie la durabilité observée du gène majeur de résistance Pvr4. Cependant, nous proposons que le mécanisme exploité par le virus pour compenser les coûts de virulence constitue également un indicateur de la durabilité des résistances.
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Identification of molecular markers linked to ms8 gene in sweet pepper (Capsicum annuum L.)
auteur
G. Bartoszewski, I. Stepien, P. Gawronski, Cesary Waszczak, Véronique Lefebvre, Alain Palloix, A. Kilian, K. Niemirowicz-Szczytt
article
14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain
resume
Nuclear ms8 gene is a single recessive gene which can be used to develop the male sterility system, applicable in sweet pepper hybrid seed production. Such a nuclear male sterility system would be more effective if molecular markers of ms8 gene were available. Thus we have made an attempt to find molecular markers linked to ms8 locus. A male sterile plant of line 320 was crossed with Elf variety in order to develop F2 mapping population 320 x Elf. Line 320 is a male sterile Bulgarian-type red fruited line and Elf is a blocky-type yellow fruited sweet pepper variety. RAPD and DArT technologies combined with BSA (Bulked Segregant Analysis) were used to identify molecular markers of ms8 gene. Seven RAPD markers linked to rns8 gene were identified and converted to dominant SCAR markers. Two developed SCAR markers were segregating in the doubled haploid population from the F1 (Yolo Wonder x Perennial) and were mapped on the lower arm of the pepper chromosome P4 COSII markers available for lower arm of chromosome P4 were tested in 320 x Elf population and two markers were segregating showing rather weak linkage with ms8 locus. DArT BSA analysis resulted in the identification of seven DArT markers potentially linked to rns8 locus. The applicability of the identified markers in the breeding programmes will be subject to further studies.
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Durable management of root-knot nematodes Meloidogyne spp. in pepper (Capsicum annuum) using resistant genotypes
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Caroline Djian-Caporalino, Alain Palloix, Ariane Fazari, Nathalie Marteu, Michel Bongiovanni, Anne-Marie Sage-Palloix, Ghislaine Nemouchi, Philippe Castagnone-Sereno
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14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain
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Providing genomic tools to increase the efficiency of molecular breeding for complex traits in pepper
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Maryse Nicolaï, Anne-Marie Sage-Palloix, Ghislaine Nemouchi, Bruno Savio, A. Vercauteren, M. Vuylsteke, Véronique Lefebvre, Alain Palloix
article
14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain
resume
The aim of the SPICY European project ("Smart tools for Prediction and Improvement of Crop Yield", KBBE-2008-211 347) is to develop a suite of tools for molecular breeding of crop plants for sustainable and competitive agriculture. The model crop is Pepper (Capsicum annuum). A crop growth model will be constructed to predict the phenotypic response of a genotype under a range of environmental conditions. Molecular markers of the Quantitative Trait Loci (QTLs) for yield-related traits and for model parameters are needed for phenotype prediction. To improve the estimation of allelic values at QTLs, functional markers (sequence polymorphism controlling the phenotypic variation) are expected instead of QTL flanking markers. The genomic part of this project exptores functions underlying QTLs by quantitative genomics using both a priori (genes reported in literature as playing an important role in growth responses) and global gene expression polymorphism that is genes that are differentially expressed in the RIL population, (eQTL) . SNPs in the genes of interest will be obtained from high-throughput sequencing and mapped in pepper genome by SNPlex using the 297 RIL population. SNP positions in the genetic map will be confronted with positions of eQTLs and trait QTLs. Colocalization of a structural gene (SNP), a trait QTL and an eQTL will argue in favour of causal relationships between the identified gene and the trait. Because functional validation cannot be achieved for many genes in pepper, validation will be attempted through genetic association in the pepper germplasm collection. Successful candidate genes will provide us with potential allelic values for phenotype prediction.
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Crop growth models for the -omics era: the EU-SPICY project
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R.E. Voorrips, Alain Palloix, A. Dieleman, Marco Bink, Egbert Heuvelink, Grégory van Der Heijden, M. Vuylsteke, Chris Glasbey, A. Barocsi, J. Magan, F. van Eeuwijk
article
14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain
resume
The prediction of phenotypic responses from genetic and environment al information is an area of active research in genetics, physiology and statistics. Rapidly increasing amounts of phenotypic information become available as a consequence of high throughput phenotyping techniques, while more and cheaper genotypic data follow from the development of new genotyping platforms. A wide array of -omics data can be generated linking genotype and phenotype. Continuous monitoring of environmental conditions has become an accessible option. This weal th of data requires a drastic rethinking of the traditional quantitative genetic approach to modeling phenotypic variation in terms of genetic and environmental differences. Where in the past a single phenotypic trait was partitioned in a genetic and environmental component by analysis of variance techniques, nowadays we desire to model multiple, interrelated and often time dependent, phenotypk t raits as a function of genes (QTLs) and environmental inputs, whi le we would like to include transcription information as well. The EU proj ect 'Smart tools for Predicti on and Improvement of Crop Yield'(KBBE2008-211347), or SPICY, aims at the development of genotype-ta-phenotype models that fully integrate genetic , genomic, physiological and environmental information to achieve accurate phenotypic predictions across a wide variety of genetic and environmental configurations. Pepper (Capsicum annuum) is chosen as the model crop, because of the availability of genetica lly characterized populations and of generic models for continuous crop growth and greenhouse production. In the presentation the objectives and structure of SPICY as well as its philosophy will be discussed.
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Public and commercial collections of heirloom eggplant and pepper : a case study
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Guillaume Roch, Jean-Paul Bouchet, Anne-Marie Sage-Palloix, M-Christin Daunay
article
14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain
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Exploratory QTL analyses of some pepper physiological traits in two environments
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Nurudeen Adeniyi Alimi, M.C. Bink, A. Dieleman, Anne-Marie Sage-Palloix, R.E. Voorrips, Véronique Lefebvre, Alain Palloix, F.A. van Eeuwijk
article
14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain
resume
The use of molecular breeding technlques has increased insight into the genetics behind phenotypic differences and led to selectlon of genotypes havlng favourable traits. Continuous monitoring of environmental conditions has also become an accessible option. Rather than single trait evaluation, we would prefer smarter approaches capable of evaluating multiple, often correlated and time dependent traits simultaneously as a function of genes (QTLs) and environmental inputs, where we would like to include intermediate genomic information as well. In this paper, an exploratory QTL analysis over two environments was undertaken using available genetic and phenotypic data from segregating recombinant inbred lines (RIL) of pepper (Capsicum annuum). We focused on vegetative traits, e.g. stem length, speed of stem development, number of internodes ete. We seek to improve the estimation of allelic values of these traits under the two environments and determine possi ble QTL x E interaction. Almost identical QTLs are detected for each trait under the two environments but with varying LOD scores. No clear evidence was found for presence of QTL by environment interactions, despite differences in phenotypes and in magnitude of QTLs expression. Within the EU project SPICY (Voorrips et al., 2010 this issue), a larger number of environments will be studied and more advanced statistical analysis tools witl be considered. The correlation between the traits will also be modelled. The identification of markers for the important QTL (Nicotaï et al., 2010 this issue) will improve the speed and accuracy of genomic prediction of these complex phenotypes.
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The virtual fruit: towards a tool to design ideotypes for substainable production systems
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Bénédicte Quilot, Mohamed Mahmoud Ould Sidi, Abdeslam Kadrani, Michel M. Génard, Francoise F. Lescourret, Nadine Hilgert
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11. ESA Congress, Aug 2010, Montpellier, France
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Genetic diversity of Tunisian apricot germplasm assessed by AFLP and SSR markers
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Lamia Krichen, Hedia Bourguiba, N. Trifi-Farah, Jean Marc Audergon
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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QTL analysis for fruit ripening traits in apricot (Prunus armeniaca L.)
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Patrick Lambert, David Ruiz, Barbara Gouble, Sylvie Bureau, Maryse Reich, Patrice Reling, Alexandre Bachellez, Catherine M.G.C. Renard, Jean Marc Audergon
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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Bayesian model clustering analysis of apricot in the mediterranean basin
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Hedia Bourguiba, Lamia Krichen, N. Trifi-Farah, Bouchaib Khadari, Aurélia Mamouni, Samia Trabelsi, C. Donofrio, J. Egea, B.M. Asma, Barbara Gouble, Jean Marc Audergon
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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More efficient conservation and use of vegetable genetic resources in Europe: ECPGR achievements and perspectives
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M-Christin Brand-Daunay, N. Bas, W. van Dooijeweert, R. van Treuren, M.J. Diez, E.R.J. Keller, K. Smékalova, C. Allender, D. Astley, F. Branca, Emmanuel E. Geoffriau, T. Kotlinska, J. Engels, L. Maggioni
article
28. International Horticultural Congress on Science and Horticulture for People (IHC2010); 3. International Symposium on Plant Genetic Resources, Aug 2010, Lisbon, Portugal
resume
The European Cooperative Programme for Plant Genetic Resources (ECPGR) is a regional network funded by the European countries and coordinated by Bioversity International. The Vegetables Network with representatives of 42 countries, is one of the crop specific ECPGR networks (http://www.ecpgr.cgiar.org/ networks/vegetables.html). It consists of six Working Groups (WGs), i.e., on Allium, Brassica, Cucurbits, Leafy Vegetables, Solanaceae and Umbellifer Crops. Sharing responsibilities for the ex situ conservation of European vegetable crops genetic resources is the highest priority of the Vegetables Network. It is foreseen that the rationalization of the collections will lead to higher cost-efficiency, and improvement of plant genetic resources documentation and quality. These efforts will be continued within the framework of the AEGIS initiative (A European Genebank Integrated System) (http://www.aegis.cgiar.org/). Challenges for the Vegetables Network include the identification of the so-called Most Appropriate Accessions (MAA) for each crop for their inclusion in the decentrally managed European Collection, and the development of agreed crop specific technical standards for conservation. Achievements of the Network in recent years include the development of European Central Crop Databases (ECCDBs), quality standards for collection man-agement of seed-propagated crops and cryopreserved material, safety duplication improvement and definition of minimum characterization descriptors. Several EU-funded projects have initiated and accelerated the activities of the WGs. Apart from further improvements within the framework of AEGIS, the Network is planning a number of other initiatives, such as improving collaboration at the global level (Allium), developing molecular characterization protocols (lettuce), filling the gaps in the conservation of wild relatives (Brassica and Umbellifer Crops), and improving the Network’s communication with the scientific community and the public at large.
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Use of different methodological approaches to study sugar contents in stonefruits
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L. Dondini, M. Adami, Stefano Tartarini, Daniele Bassi, David Ruiz, Patrick Lambert, Thierry Pascal, Bénédicte Quilot, Jean Marc Audergon, Barbara Gouble, Sylvie Bureau, Maryse Reich, Catherine M.G.C. Renard, Christel Renaud, Elisabeth Dirlewanger, Mickael M. Maucourt, Catherine Deborde, Annick Moing, E. Illa, W. Howad, Pere Arus
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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Inheritance of fruit flesh phenolic compounds in peach and apricot: identification quantitative variations and QTL comparative mapping
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Camille Croset, Jean Marc Audergon, Marie Noëlle Corre, Patrick Lambert, Bénédicte Quilot, Barbara Gouble, Sylvie Bureau, L. Dondini, Stefano Tartarini, Christel Renaud, Elisabeth Dirlewanger, Pere Arus, Jean-Luc Poessel
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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Genetic structure of Mediterranean apricots by SSR fingerprinting
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Hedia Bourguiba, Lamia Krichen, Neïla Trifi-Farah, Bouchaib Khadari, Ali Mamouni, Samia Trabelsi, Claudio d'Onofrio, José Egea-Caballero, David Ruiz, B.M. Asma, Barbara Gouble, Jean Marc Audergon
article
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources, Aug 2010, Lisbon, Portugal. ⟨10.17660/ActaHortic.2011.918.39⟩
resume
Apricot (Prunus armeniaca L.) is an important fruit species especially in the Mediterranean bassin area. The genetic structure of apricot cultivars in this region was studied in order to shed light on the evolutionary history of this species. A sample of 207 patrimonial accessions was collected from different Mediterranean countries: Algeria, France, Italy, Morocco, Tunisia, Turkey, and Spain. Molecular characterization was performed using 25 SSR markers, selected according to high level of polymorphism, transferability between Prunus species, and localisation over the eight linkage groups of the Prunus genome. Statistical treatment of the dataset was undertaken using a model-based Bayesian clustering analysis implemented in the STRUCTURE program. It allowed assignment of the studied accessions into four distinct genetic clusters (Fst=0.122). A gradient of decreasing genetic diversity from the east to the south-west of the Mediterranean area was also evidenced. An interesting new pattern based on the discrimination of a ‘Maghreb’ cluster was revealed for the first time. These results clearly open new research lines dealing with the establishment of a nested core collection at the Mediterranean level, and with the prospective development of genetic association studies.
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Rapid characterization of apricot fruit quality using near and mid-infrared spectroscopy: Study of the model robustness
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Sylvie Bureau, Maryse Reich, Catherine M.G.C. Renard, D. Ruiz, Jean Marc Audergon
article
28. International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Postharvest Technology in the Global Market, Aug 2010, Lisbonne, Portugal. ⟨10.17660/ActaHortic.2012.934.20⟩
resume
The objective of this work was to evaluate the robustness of Near Infrared (NIR) and Mid-Infrared (MIR) models on a set of apricots with large phenotypic variability (eight contrasted cultivars, four maturity stages, and 180 hybrids from a cross between 'Goldrich' and 'Moniqui'). The error of prediction obtained by NIR models on intact fruit was 9.2% for soluble solids content (SSC) and 16.5% for titratable acidity (TA). Using MIR models on slurries, this error was 6.1% for SSC and 8.6% for TA. Overall, the prediction performance of models established using large phenotypic variability was stable enough for global quality traits such as SSC and TA.
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QTLs involved in phenological traits in several Prunus species (peach, apricot and sweet cherry)
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Elisabeth Dirlewanger, Maria-Das-Dores Lafargue, Jacques Joly, Yves Tauzin, Bénédicte Quilot, Patrick Lambert, David Ruiz, Jean Marc Audergon, L. Dondini, Stefano Tartarini, E. Illa, Pere Arus, José Quero-Garcia
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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Large Scale Phenotyping and Data Analysis of Pepper Genotypes in the EU-SPICY Project
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J.A. Dieleman, J.J. Magan, A.M. Wubs, Alain Palloix, S. Lenk, C. Glasbey, F.A. van Eeuwijk
article
28. International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Cropsti, Aug 2010, Lisbonne, Portugal. 446 p
resume
In breeding the best genotypes for diverse conditions, ideally the breeder should test all his crossings under all these conditions. Especially with complex physiological traits like yield, which exhibit large variation, this would require many expensive and large field trials. The EU project “Smart tools for Prediction Improvement of Crop Yield” (SPICY) aims at the development of a suite of tools to help breeders in predicting phenotypic response of genotypes for complex traits under a range of environmental conditions. Pepper is used as a model crop. The primary tool to be developed is an integrated gene-to-phenotype model, which integrates the approaches of QTL and crop growth modelling. To generate data to develop and validate these models, a recombinant inbred line (RIL) population of Capsicum annuum ‘Yolo Wonder’ × ‘Criollo de Morelos 334’ was phenotyped at two sites (Netherlands and Spain) and in two seasons (spring and autumn). Phenotyping was done both manually by measuring characteristics like fruit set and development rate, and by newly developed image analysis and fluorescence tools permitting high throughput phenotyping of dynamic trait expression. In the analysis of the data, the complex trait yield was analysed in the components contributing to the total fresh fruit yield. The results of the phenotyping experiments will be used as input in the crop growth models. The genotype-to-phenotype model, combination of crop growth models and QTL analysis tools which will be developed in the EU-SPICY project will be able to predict phenotypic response based on genetic information
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The new EU project FruitBreednomics: an integrated approach for increasing breeding efficiency in fruit tree crops
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Francois Laurens, M.J. Aranzana, Pere Arus, Joan Bonany, L. Corelli-Grappadelli, Bao Diep, Andrea Patocchi, Andreas Peil, Bénédicte Quilot-Turion, Silvio Salvi, A. Vecchietti, E. van de Weg
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28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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A tentative typology of apricot fruit maturation
auteur
Barbara Gouble, Saadeddine Ben Ali, Guy Clauzel, Sylvie Bureau, Patrice Reling, Catherine M.G.C. Renard, Jean Marc Audergon
article
28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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titre
Genetic analysis of peach and apricot fruit characters based on QTL analysis and candidate gene
auteur
Pere Arus, Bénédicte Quilot, E. Illa, Loick Le Dantec, Annick Moing, Patrick Lambert, Jean-Luc Poessel, Jean Marc Audergon, L. Dondini, Stefano Tartarini, W. Howad, Elisabeth Dirlewanger
article
28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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titre
More efficient conservation and use vegetable genetic resources in Europe: ECPGR achievements
auteur
M-Christin Daunay, C. Allender, D. Astley, N. Bas, Francesco Branca, M.J. Diez-Niclos, W. van Dooijeweer, Emmanuel E. Geoffriau, J. Keller, T. Kotlinska, K. Smekalova, R. van Treuren, J.L. Engels Maggioni
article
28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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titre
Rapid characterization of apricot fruit quality using near and mid-infrared spectroscopy: study of the model robustness
auteur
Sylvie Bureau, David Ruiz, Maryse Reich, Jean Marc Audergon, Catherine M.G.C. Renard
article
28. International Horticultural Congress, Aug 2010, Lisboa, Portugal. 1 p
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titre
Infrared spectroscopy used for the fruit quality characterisation of apricot hybrids from a cross between two constrasted cultivars
auteur
Sylvie Bureau, David Ruiz, Maryse Reich, Barbara Gouble, Jean Marc Audergon, Catherine M.G.C. Renard
article
28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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titre
Development of tools to improve prediction of crop yield
auteur
J.A. Dieleman, F. van Eeuwijk, Egbert Heuvelink, Alain Palloix, M. Vuylsteke, Chris Glasbey, G. Heijden, Marco Bink, R.E. Voorrips, A. Barocsi, J.J. Magan
article
28. International Horticultural Congress, Aug 2010, Lisboa, Portugal
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titre
Estimating risk factors for PPV dissemination from incomplete epidemiological surveillance data
auteur
David Pleydell, Gael Thébaud, Samuel S. Soubeyrand, Sylvie Dallot, Sylvain Grizard, Joel J. Chadoeuf, Gerard Labonne
article
International Symposium on Plum Pox Virus, Aug 2010, Sofia, Bulgaria
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titre
La résistance des plantes cultivées aux insectes phloémophages : bases génétiques et moléculaires
auteur
Catherine Dogimont
article
7. Conférence Internationale Francophone d'Entomologie, Jul 2010, Louvain la Neuve, Belgique
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titre
Glycoalcaloid contents in a family from a cross between a diploid S. tuberosum and a diploid S. sparsipilum clone are under polygenic control
auteur
Marie-Claire Kerlan, E. Bonnel, Sylvie Marhadour, Bernard Caromel, Jean-Eric Chauvin
article
EAPR-EUCARPIA congress, Jun 2010, Wageningen, Netherlands
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titre
Genetic and molecular control of organoleptic quality components in tomato
auteur
Mathilde M. Causse
article
Gordon Research Conference Postharvest Physiology, Jun 2010, Tilton, United States. 1p
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titre
A mutation in the NIb cistron of Potato virus Y confers virulence towards the Pvr4 resistance of pepper and a high competitiveness cost in susceptible cultivar
auteur
Bérenger Janzac, Josselin Montarry, Alain Palloix, Olivier Navaud, Benoît Moury
article
11. International Plant Virus Epidemiology Symposium, Jun 2010, Ithaca, United States
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https://hal.inrae.fr/hal-02751150/file/36012_20101103122844551_1.pdf BibTex
titre
Host factors required for plant susceptibility to viruses: targets to improve plant resistance to viruses
auteur
Carole Caranta, Laurence Ouibrahim, Séverine Lacombe, Christophe Robaglia, Jean-Luc J.-L. Gallois
article
EMBO WORKSHOP 2010, Jun 2010, Fenestrelle, Italy
resume
Because of their small genome encoding generally less thari a dozen proteins the viral infectious cycle completely relies on the use of cellular factors. The completion of the viral cycle is therefore the result of a complex interplay between virus-encoded and host-encoded factors. ln this scheme, absence or mis-adequacy of a single host factor (also called susceptibility factors) led to plants fully or partially resistant to viruses. ln the plant natural diversity, these non-functional versions of host factors required for the viral cycle were demonstrated to correspond to recessive resistance genes. ln recent year, one of the results that has enabled plant virology to make a step forward is the identification of components of the translation initiation complex as essential host factors required for RNA virus infection. Although translation initiation factors were demonstrated to be highly conserved determinants of plant resistance to viruses, several data indicate that the molecular basis underlying translation initiation factors-mediated resistance are highly variable. ln parallel, several recessive resistance genes against viruses were identified and demonstrated to be distinct from translation initiation factors. These genes are therefore very good candidates for the discovery of new susceptibility factors. Recent results on (i) the specificity in the use of translation initiation factors by RNA viruses and on (ii) the identification of new host susceptibility factors, will illustrate this to ic.
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Conference papers
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https://hal.inrae.fr/hal-02754753/file/Caranta_Gallois%202010%20Embo_1.pdf BibTex
titre
Development of a bac library and bac end sequencing for pedunculate oak (Quercus robur L.) : new tools for oak genome studies
auteur
Patricia P. Faivre-Rampant, Frederique Bitton, Clément Boussardon, Catherine Bodenes-Brezard, Emilie E. Chancerel, Grégoire G. Le Provost, Jean-Marc Frigerio, Cyril Cabane, Frédéric Gavory, Antoine Kremer, Christophe Plomion
article
Conference on Forest Ecosystem Genomics and Adaptation, Jun 2010, Madrid, Spain
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titre
Genes flow in populations of Bremia lactucae, the causal agent of downy mildew of lettuce, in France
auteur
Romain R. Valade, Brigitte B. Maisonneuve, Claire C. Neema
article
Congrès du réseau de Génétique Moléculaire des Oomycètes, Jun 2010, Toulouse, France
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https://hal.inrae.fr/hal-02750444/file/40009_20100610081515958_1.pdf BibTex
titre
Programme d'amélioration génétique du pêcher (INRA)
auteur
Thierry Pascal, Patrick Lambert, Jean-Luc Poessel, Véronique Decroocq, Marie-Helene Sauge-Collet
article
12. Colloque Scientifique de la Société Nationale d'Horticulture de France (SNHF) : 'L'Arbre Vulnérable mais Durable’, Société Nationale d'Horticulture de France (SNHF). FRA., May 2010, Paris, France. 56 p
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titre
Chromosome landing at a quantitative trait locus involved in resistance to cyst nematode in potato
auteur
Bernard Caromel, Marie-Claire Kerlan, Patricio Castro Quezada, Jawad Aarrouf, Helene Berges, Didier Mugniery, Véronique Lefebvre
article
4. Annual Meeting; COST 872 workshop, May 2010, Lisbon, Portugal
resume
Chromosome landing at a quantitative trait locus involved in resistance to cyst nematode in potato. 4. Annual Meeting; COST 872 workshop
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https://hal.science/hal-01462645/file/49322_20120208085006382_1.pdf BibTex
titre
Chromosome landing at a quantitative trait locus involved in resistance to cyst nematode in potato.
auteur
Bernard Caromel, Marie-Claire Kerlan, Paulo Castro, Jawad Aarrouf, Helene H. Berges, Didier Mugniery, Véronique Lefebvre
article
Cost 872 Workshop & MC Meeting,, May 2010, Lisbonne, Portugal
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titre
Caractérisation des interaction plantes/oomycètes: approches génomiques et transcriptomiques de l'interaction et résistances quantitatives des Solanacées aux oomycètes
auteur
Véronique Lefebvre, Franck Panabières
article
1. Journées Scientifiques Inra PACA, Apr 2010, Cogolin, France
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titre
Opportunities of genetic association studies in pepper: estimation of the genetic structure and linkage disequilibrium in a germplasm sample
auteur
Melissa Cantet, Stephanie Mallard, Maryse Nicolaï, Anne-Marie Sage-Palloix, Véronique Lefebvre
article
2. International Symposium on Genomics of Plant Genetic Resources, Apr 2010, Bologne, Italy
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Conference papers
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https://hal.inrae.fr/hal-02751906/file/33647_20100517090213387_1.pdf BibTex
titre
Monipech: limiter la moniliose en verger en associant génotypes résistants et opérations culturales raisonnées. Quel rôle pour les composés phénoliques
auteur
Bénédicte Quilot-Turion, Jean-Luc Poessel
article
Journées métabolites secondaires, pôle PHI, Apr 2010, Avignon, France
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titre
Cloning candidate genes for a broad-spectrum resistance QTL against Phytophthora capsici in pepper
auteur
Melissa Cantet, Véronique Lefebvre, Stéphane Mallard, Abdelhafid A. Bendahmane, Adnane Boualem, Christelle Troadec, Helene H. Berges, Sonia S. Vautrin
article
Plant Genomics seminar 2010, Mar 2010, Mallemort, France. 2 p
resume
Late blight, caused by the pathogen Phytophthora spp. (Oomycete), is one of the most damaging diseases of Solanaceae. The deployment of resistant varieties is the most effective, economical and environmentally safe control of Phytophthora. Contrary to monogenic resistances that are easily overcome by the pathogen, polygenic resistances generally confer a durable control of disease severity. However, their molecular basis remains unknown since no resistance QTl to Phytophthora has ever been characterized. Phy-P5, the major effect Phytophthora capsici resistance QTl in pepper, has been mapped on the chromosome P5. It was detected in 3 pepper accessions and displayed a broad-spectrum resistance to several P. capsici isolates. We airn to determine the molecular basis of Phy-PS by isolating the responsible gene deploying a positional cloninq strategy.
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titre
PROTIC workshop: A bioinformatics environment for proteome data validation, analysis and integration
auteur
Renaud Flores, Johann J. Joets, Olivier Langella, Benoit B. Valot, Michel M. Zivy, Antoine de Daruvar, L. Gil, D. Jacob, Jean-Paul Bouchet, Mireille Faurobert, Dominique Jeannin, Karine Leyre, Céline C. Lalanne, Christophe Plomion, Delphine D. Vincent
article
Plant Genomics seminar 2010, Mar 2010, Pont Royal en Provence, France
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Conference papers
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https://hal.inrae.fr/hal-02758409/file/Faurobert%202010%20Genoplante%2072-73_1.pdf BibTex
titre
QTL GpaVsspI Pdt/nématodes
auteur
Bernard Caromel
article
Rencontres Scientifiques du DGAP, Mar 2010, La Colle sur Loup, France
typdoc
Conference papers
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https://hal.inrae.fr/hal-02815542/file/Caromel%202010%20Rencontres%20DGAP%2033_1.pdf BibTex
titre
Diversité SSR des collections Aubergine et Piment
auteur
Marie-Christine Daunay
article
Rencontres Scientifiques du DGAP, Mar 2010, La Colle sur Loup, France. 1 p
resume
Objectifs du projet: structurer avec 20 SSR "aubergine" une partie de la collection d'aubergine de l'INRA (la partie "aubergine" a été réduite de moitié, et la partie "piment" a été sortie du projet du fait du budget alloué très inférieur au budget demandé).
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Conference papers
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https://hal.inrae.fr/hal-02824898/file/Brand-Daunay%202010%20Rencontres%20DGAP%2042_1.pdf BibTex
titre
Providing genomic tools to improve phenotype prediction for complex traits
auteur
Maryse Nicolaï, Nurudeen Adeniyi Alimi, Anne-Marie Sage-Palloix, Ghislaine Nemouchi, Bruno Savio, Véronique Lefebvre, M. Vuylstecke, Marco Bink, Fred van Eeuwijk, Alain Palloix
article
International Conference, Feb 2010, Vienne, Austria
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Conference papers
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https://hal.inrae.fr/hal-02757361/file/34368_20100428020151614_1.pdf BibTex
titre
Les apports de la génétique pour la qualité de la tomate
auteur
Mireille Faurobert
article
Journée Qualité et consommation de la tomate, Feb 2010, Plan d'Orgon, France. 14 diapos
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Conference papers
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https://hal.inrae.fr/hal-02823865/file/35792_20100517080454196_1.pdf BibTex
titre
Integrating genetic information into an ecophysiological model: example of peach fruit quality
auteur
Bénédicte Quilot-Turion, Michel M. Génard
article
Indo French Symposium, Jan 2010, Lucknow, India
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Conference papers
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https://hal.inrae.fr/hal-02755910/file/34524_20100401092430366_1.pdf BibTex
titre
Genetic and genomic approach of tomato fruit quality
auteur
Mathilde M. Causse, Mireille Faurobert, Jamila Chaïb, Stephane Munos, Nicolas N. Ranc
article
Indo French Symposium, Jan 2010, Lucknow, India
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Conference papers
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https://hal.inrae.fr/hal-02754042/file/36834_20100401083208384_1.pdf BibTex
titre
Genetic and molecular diversity of fruit traits in melon
auteur
Catherine Dogimont
article
Indo French Symposium, Jan 2010, Lucknow, India
typdoc
Conference papers
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https://hal.inrae.fr/hal-02750884/file/37649_20100401095112261_1.pdf BibTex
titre
Candidate gene mapping and QTL analysis involved in agronomical and fruit quality traits in Prunus species in the European integrated project ISAFRUIT
auteur
Elisabeth Dirlewanger, Gaëlle Cardinet, Christel Renaud, Loick Le Dantec, Catherine Deborde, Mickael M. Maucourt, Annick Moing, Camille Croset, Patrick Lambert, Jean-Luc Poessel, David Ruiz, Bénédicte Quilot, Jean Marc Audergon, Barbara Gouble, Sylvie Bureau, Maryse Reich, Patrice Reling, Stefano Tartarini, Luca Dondini, Eudald Illa
article
International Plant & Animal Genomes Conference, Jan 2010, San Diego, United States
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titre
New genotype to phenotype models at the intersection of genetics, physiology and statistics; smart tools for prediction and improvement of cropyield
auteur
Fred van Eeuwijk, Anja Dieleman, Alain Palloix, Marnik Vuylsteke, Chris Glasbey, Attila Barocsi, Juan Jose Magan Canadas, Ep Heuvelink, Gerie van Der Heijden, Eric van de Weg, Roeland Voorrips, Marco Bink
article
18. Plant & Animal Genome, Jan 2010, San Diego, United States
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titre
Sex determination in curcubits: genetic and epigenetic implications
auteur
A Martín, Christelle Troadec, Adnane Boualem, Mazen Rajab, Romain Fernandez, Halima H. Morin, Michel Pitrat, Catherine Dogimont, Abdelhafid A. Bendahmane
article
Plant and Animal Genomes XVIII Conference, Jan 2010, San Diego, United States
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titre
Séquençage d'un clone BAC de pomme de terre, afin de développer des marqueurs moléculaires pour la cartographie à haute résolution et l'atterrissage chromosomique au locus GpaVspl, impliqué dans la résistance au nématode à kyste Globodera pallida chez la pomme de terre
auteur
Bernard Caromel
article
Les rencontres scientifiques du DGAP, 2010, La Colle sur Loup, France
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Poster communications

titre
Pericarp histology and cell wall chemistry of tomato lines with altered fruit firmness
auteur
Marc Lahaye, Bernard Quemener, Marie Francoise M. F. Devaux, M. Pool, Mathilde M. Causse, G. Seymour
article
7. Solanaceae Conference (SOL2010), Sep 2010, Dundee, United Kingdom. 2010
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titre
Dynamique de l'adaptation de Bremia lactucae face aux résistances variétales de la plante hôte
auteur
Romain R. Valade, Claire C. Neema, Brigitte B. Maisonneuve
article
Colloque national d'écologie scientifique, Sep 2010, Montpellier, France. 2010, Ecologie 2010
typdoc
Poster communications
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https://hal.inrae.fr/hal-02751391/file/34963_20101124120149731_1.pdf BibTex
titre
Impact of genotype, fruit ripening and UV-B radiations on phenolic profile of cherry tomato fruits
auteur
Michele Loonis, Nathalie Mora, Marie Noëlle Corre, Hélène Gautier, Jean-Luc Poessel, Claire Dufour
article
25. International Conference on Polyphenols, Aug 2010, Montpellier, France. 2010
resume
Tomato or Solanum lycopersicum is one of the most widely grown fruits for consumption either as raw or cooked. Beneficial health effects have been attributed to its high content in micronutrients such as carotenoids, mainly lycopene and β-carotene, and vitamin C. Phenolic compounds, which constitute the most diversified class of tomato micronutrients, participate to the fruit quality by their contribution to the colour, taste and aroma, and, owing to their antioxidant properties, could also contribute to healthpromoting effects of tomato consumption. Different studies have shown that total phenolic content of tomato fruit is greatly affected by the genotype, stage of ripening and environmental factors. There is an increasing interest to study the variations of individual compounds according to these factors, especially in cherry tomatoes which are often used in genetic improvement for their high organoleptic quality.
typdoc
Poster communications
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https://hal.inrae.fr/hal-02758492/file/Poessel_2010%2025.%20Int.%20Conf.%20Poly.%20Poster_1.pdf BibTex
titre
The genetic basis of adaptation in Arabidopsis lyrata
auteur
Helmi Kuittinen, Bénédicte Quilot, Paivi H. Leinonen, Johanna Leppälä, Outi Savolainen
article
Annual Meeting of the Society for Molecular Biology and Evolution (SMBE), Jul 2010, Lyon, France. 1 p., 2010
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02816703/file/50860_20120227124455049_1.pdf BibTex
titre
Méthodes d'échantillonnage du mildiou de la laitue en vue d'analyser les populations françaises de cet oomycète
auteur
Brigitte B. Maisonneuve, Loic Jean
article
8. Rencontres de Phytopathologie-Mycologie de la Société Française de Phytopathologie (SFP), Jan 2010, Aussois, France. 2010, Journées Jean Chevaugeon 2010
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02752958/file/35227_20100614083852592_1.pdf BibTex
titre
Structure des populations de Bremia lactucae, l'agent du mildiou de la laitue en France
auteur
Romain R. Valade, Brigitte B. Maisonneuve, Claire C. Neema
article
8. Rencontres de Phytopathologie - Mycologie de la Société Française de Phytopathologie (SFP), Jan 2010, Aussois, France. 2010, Journées Jean Chevaugeon 2010
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02751992/file/42816_20111028025104568_1.pdf BibTex

Reports

titre
Écophyto R&D : Quelles voies pour réduire l’usage des pesticides ? Résumé de l'étude
auteur
Jean-Pierre Butault, Charles-Antoine Dedryver, Christian Gary, Laurence Guichard, Florence Jacquet, Jean-Marc Meynard, Philippe C. Nicot, Michel Pitrat, Raymond Reau, Benoit Sauphanor, Isabelle Savini, Therese Volay
article
[Rapport de recherche] INRA; Ecophyto. 2010, 8 p
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Reports
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https://hal.science/hal-03277913/file/EcophytoR%26D_R%C3%A9sum%C3%A9.pdf BibTex
titre
Caractérisation de mutants d'Arabidopsis pour les facteurs d'initiation de la traduction : impact sur la résistance aux Potyvirus
auteur
Marie Salvini
article
[Stage] Faculté des Sciences de Luminy. Université de la Méditerranée (Aix Marseille 2), Marseille, FRA. 2010, 30 p
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Reports
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https://hal.inrae.fr/hal-02814794/file/45380_20110722085028686_1.pdf BibTex
titre
SOLstlS: Services Web et Infoservices en Protéomique Végétale
auteur
Elise Larsonneur
article
[Stage] Université d'Évry-Val-d'Essonne (UEVE), Evry, FRA. 2010, 26 p. + 9 p. annexes
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titre
Identification du (des) gène(s) lié(s) au QTL contrôlant le rapport des concentrations fructose/glucose dans la pêche
auteur
Mathieu Cammas
article
[Stage] France. Université Claude Bernard Lyon 1 (UCBL), Villeurbanne, FRA. 2010, 27 p
resume
Un phénotype particulier de pêcher présentant des fruits déficitaires en fructose à été détecté dans une population interspécifique de pêcher (Prunus davidiana x Prunus persica). Ce phénotype particulier ce retrouve chez environ ¼ des individus de cette population (le reste de la population présentant des quantités de fructose habituellement rencontrées dans les pêches commerciales) et semble être contrôlé par un QTL cartographié sur le chromosome 1 du génome du pêcher. Nous avons tenté de déterminer le gène ou le groupe de gènes liés à ce QTL par une démarche dite de gène candidat. Les candidats positionnels on été recherché parmi les gènes annotés lors du séquençage du génome du pêcher (données disponibles depuis le 1er mai 2010). Des marqueurs microsatellite ont été défini pour réduire la taille de la portion du génome sensée contenir ce QTL afin de limiter le nombre de gènes candidats. Les gènes candidats fonctionnels ont été choisis parmi les gènes codant des enzymes du métabolisme du fructose avec l’appui d’analyses d’activités enzymatiques. La zone de présence du QTL initialement d’environ 1500kb à pu être réduite à une séquence de moins de 500kb contenant théoriquement 160 gènes environ. L’annotation actuelle de cette portion du génome ne comprend que 51 gènes. Un gène codant pour une phosphofructokinase pyrophosphate dépendante (PFP) à été identifié sur le chromosome 5. Bien que n’étant pas un candidat dans cette étude, il à été tenté d’étudier les séquences de ses différents allèles présents dans la population afin de déterminer son implication dans un différentiel d’activité enzymatique observé entre les deux phénotypes étudiés. L’annotation plus en détail des gènes présents au niveau du QTL et la recherche de candidats autre que des enzymes du métabolisme du fructose (transporteurs de sucre, protéines régulatrices…) semble une bonne voie pour orienter la suite des recherches.
typdoc
Reports
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02818496/file/46486_20110720034533733_1.pdf BibTex
titre
Écophyto R&D : quelles voies pour réduire l’usage des pesticides ? Synthèse du rapport de l'étude
auteur
Jean-Pierre Butault, Charles-Antoine Dedryver, Christian Gary, Laurence Guichard, Florence Jacquet, Jean-Marc Meynard, Philippe C. Nicot, Michel Pitrat, Raymond Reau, Benoit Sauphanor, Isabelle Savini, Therese Volay
article
[Rapport Technique] Ministère de l'Ecologie, de l'Energie, du Développement Durable et de la Mer. 2010, 90 p
resume
La loi de programmation relative au Grenelle de l’environnement fixe l’objectif de réduire de moitié les usages des produits phytopharmaceutiques en 10 ans en accélérant la diffusion de méthodes alternatives sous réserve de leur mise au point. Dès 2007, à la demande des ministères en charge de l’environnement et de l’agriculture, l’Inra a lancé une étude sur les itinéraires culturaux économes en pesticides. Quatre filières ont été prises en compte : grandes cultures, arboriculture fruitière, viticulture et cultures légumières. Pour chacune d’elles, différentes stratégies de limitation du recours aux produits phytosanitaires ont été analysées, au vu des connaissances disponibles. L’impact économique et environnemental du recours à ces stratégies a été évalué, au niveau national (en France métropolitaine), pour différents scénarios. L’étude comporte également une analyse des jeux d’acteurs en amont et en aval des filières agricoles face à la réduction d’usage des pesticides. L'étude a par ailleurs défini les modalités de conception d’un réseau d’acquisition et de diffusion de références agronomiques. Ces résultats seront mobilisés pour la mise en place du réseau de fermes de références, d’expérimentation et de démonstration prévu par le plan ministériel Ecophyto 2018. Ecophyto R&D a mobilisé une centaine d’experts issus de plus de 30 organismes, parmi lesquels l'Inra, des établissement d'enseignement supérieur, des orgnaismes de développement agricole, les services techniques des ministère, etc. Les résultats ont pour fonction d'éclairer la décision publique et d'identifier des moyens pour la mettre en oeuvre. Ils ne constituent pas une source directement utilisable pour piloter des changements techniques au niveau local ou au niveau d'une exploitation agricole.
typdoc
Reports
Accès au texte intégral et bibtex
https://hal.science/hal-01172967/file/38087_20100212033830140_1.pdf BibTex

2009

Conference papers

titre
Specificity of Gafsa oasis on the genetic structure of apricot (Prunus armeniaca L.) germplasm
auteur
Hedia Bourguiba, Lamia Krichen, Jean Marc Audergon, N. Trifi-Farah
article
3. Meeting international sur l'aridoculture et les cultures oasiennes, Dec 2009, Djerba, Tunisia
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titre
Proteomic analysis of salt-stressed tomato (Solanum lycopersicum)
auteur
Arafet Manaa, Mireille Faurobert, Salwa Mouelhi, Samira Smiti, Ezzeddine Zid, Hela Ben Ahmed
article
3. Meeting international sur l'aridoculture et les cultures oasiennes, Dec 2009, Djerba, Tunisia
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titre
Working group on Solanaceae
auteur
Willen van Dooijeweert, M-Christin Daunay
article
3. meeting Vegetables Network, Nov 2009, Catania, Italy
typdoc
Conference papers
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https://hal.inrae.fr/hal-02754615/file/50922_20120402020540539_1.pdf BibTex
titre
QTL meta-analysis for late blight resistance in potato including the two novel resistance sources Solanum sparsipilum and S. spegazzinii
auteur
Véronique Lefebvre, Sarah Danan, Patrick Signoret, Jean-Baptiste Veyrieras
article
6. Solanaceae Genome Workshop, Nov 2009, New Delhi, India
typdoc
Conference papers
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https://hal.inrae.fr/hal-02751899/file/35889_20100428090851699_1.pdf BibTex
titre
PHY-P5, a major broad-spectrum resistance qtl conferring resistance to Phytophthora in pepper
auteur
Stephanie Mallard, Sophie Ewert, Anne Blattes, Véronique Lefebvre
article
6. Solanaceae Genome Workshop, Nov 2009, New Delhi, India
typdoc
Conference papers
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https://hal.inrae.fr/hal-02755733/file/36775_20100428100520905_1.pdf BibTex
titre
Ascorbate levels and tomato cold storage
auteur
Barbara Gouble, Noé Gest, Rebecca Stevens, Patrice Reling, Cecile Garchery, Mathilde M. Causse, Catherine C.M.G.C Renard, David Page
article
 FAVHealth, Oct 2009, Avignon, France. 384 p
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titre
GDP-d-mannose 3,5-epimerase (GME) plays a key role at the intersection of ascorbate and non-cellulosic cell-wall biosynthesis in tomato
auteur
Louise Gilbert, Moftah Alhagdow, Adriano Nunes-Nesi, Bernard Quemener, Fabienne Guillon, Brigitte B. Bouchet, Mireille Faurobert, Barbara Gouble, David Page, Virginie V. Garcia, Johann J. Petit, Rebecca Stevens, Mathilde M. Causse, Alisdair Fernie, Marc Lahaye, Christophe Rothan, Pierre Baldet
article
EUSOL Workshop, Oct 2009, Toledo, Spain
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titre
Evolution of plant fluorometry to intelligent fluorosensor concept for high troughput screening of 150 pepper genotypes
auteur
Alain Palloix
article
Workshop "Leaf Optics 2009", Oct 2009, Ebernburg, Germany
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titre
Use of EcoTILLING to identify natural allelic variants of melon candidate genes involved in fruit ripening plant gems
auteur
C Esteras, Laura Pascual-Banuls, M Saladie, Catherine Dogimont, J Garcia-Mas, F Nuez, B Pico
article
Plant Genomics European Meeting, Oct 2009, Lisbonne, Portugal
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titre
Genetic variability in apricot cell wall texture componants
auteur
Xavier Falourd, Bernard Quemener, Marie Francoise M. F. Devaux, Jean Marc Audergon, Marc Lahaye
article
4. Isafruit General Assembly, Sep 2009, Angers, France
typdoc
Conference papers
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https://hal.inrae.fr/hal-02817999/file/Isafruit2009Angers_1.pdf BibTex
titre
Insights about the higher durability of polygenic over monogenic plant resistances to viruses
auteur
Benoît Moury, Valérie Ayme, Bérenger Janzac, Alain Palloix
article
Conférence Jacques-Monod. Understanding emergence of infectious diseases: focus on new experimental and theoretical approaches to virus evolution, Sep 2009, Roscoff, France. 1 p
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titre
Development of modular fluorometers and compact fluorosensors, and their applications in plant science
auteur
Alain Palloix
article
11. International Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes, Sep 2009, Budapest, Hungary
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titre
Effect of postglacial colonization on genetic architecture of flowering and morphology in A. Lyrata
auteur
Helmi Kuittinen, Bénédicte Quilot-Turion, Païvi Leinonen, Johanna Leppälä, Anne Niittyvuopio, Outi Savolainen
article
AMEGO meeting, Aug 2009, Reykjavik, Iceland
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titre
Tacuinum Sanitatis: medieval horticulture and health
auteur
Jules Janick, M-Christin Daunay, Harry Paris
article
ASHS Annual Conference, Jul 2009, St Louis, United States
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titre
Exploration of late blight quantitative resistance in potato diversity: QTL meta-analysis including the novel resistance sources Solanum sparsipilum and Solanum spegazzinii
auteur
Véronique Lefebvre, Sarah Danan, Jean-Baptiste Veyrieras, Patrick Signoret, Bernard Caromel, Jean-Eric Chauvin
article
14. International Congress on Molecular Plant-Microbe Interactions, Jul 2009, Quebec, Canada
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titre
Fruit metabolome changes of antisens transformants of fruit specific phosphenolpyruvate carboxylase (S1-ppc2) under various environmental conditions
auteur
Cécile Cabasson, Martine Dieuaide Noubhani, Y. Ben Akal-Ben Fatma, Mickael M. Maucourt, Catherine Deborde, B. Beauvois, Marie-Hélène Andrieu, Aline Le Menn, J.L. Giraudel, Annick Moing
article
8. Colloque national de la SFBV, Jul 2009, Strasbourg, France
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titre
Gene and metabolite regulatory network analyses in tomato fruit
auteur
Annick Moing, Fabien F. Mounet, Catherine Deborde, Mickael M. Maucourt, Johann J. Petit, Virginie V. Garcia, Stéphane Bernillon, Gwanaelle Le Gall, Ian J. Colquhoun, Marianne Defernez, Jean Luc Giraudel, Rebecca Stevens, Pierre Baldet, Mireille Faurobert, Mathilde M. Causse, Christophe Rothan, Martine Lemaire Lemaire-Chamley, Dominique D. Rolin
article
Metabomeeting 2009, Jul 2009, Norwich, United Kingdom
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titre
Is Phy-P5 a major broad-spectrum resistance QTL conferring resistance to Phytophthora in Solanaceae?
auteur
Stephanie Mallard, Sophie Ewert, Anne Blattes, Caroline Callot, Morgane Ardisson, Véronique Lefebvre
article
14. International Congress on Molecular Plant-Microbe Interactions, Jun 2009, Quebec, Canada
typdoc
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titre
French peach genetic improvement program: news and perspectives for the resistance to the pests and diseases
auteur
Thierry Pascal, Patrick Lambert, Jean-Luc Poessel, Véronique Decroocq, Marie-Hélène Sauge
article
7. International Peach Symposium, Jun 2009, Lleida, Spain
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titre
Management of peach tree reference collections: ongoing research & development project relevant to the community plant variety rights protection system
auteur
Chrystelle Jouy, M.H. Gandelin, Cecile C. Collonnier, Christelle Guitouni, David Zhang, Stéphane Fouilloux, Thierry Pascal, Christophe Tuero, Z.S. Füstös, Z.S. Szani, L. Zubor, P. Chomé, M.T. Badenes, G. Soler, J. Martinez, J.M. Alonso, Ignazio Verde, M.T. Dettorri, G. Della Strada, S. Semon
article
7. International Peach Symposium, Jun 2009, Lleida, Spain
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titre
Genetic diversity of the melon aphid Aphis gossypii Glover in different melon growing areas of France
auteur
Sophie Thomas, Pascale Mistral, Virginie Chareyron, Bastien Barral, Nathalie Boissot, Flavie Vanlerberghe-Masutti
article
8. International Symposium on Aphids, Jun 2009, Catania, Italy
typdoc
Conference papers
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https://hal.inrae.fr/hal-02752596/file/27461_20090625114957977_1.pdf BibTex
titre
Mapping a large set of candidate genes related with fruit quality in peach
auteur
E. Illa, Pere Arus, Elisabeth Dirlewanger, Loick Le Dantec, Gaëlle Cardinet, Patrick Lambert, Jean Marc Audergon, W. Howad
article
7. International Peach Symposium, Jun 2009, Lleida, Spain. 1 p
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titre
Analysis of a Prunus persica [(L.) Batsch] x (P. persica x P davidiana) progeny for the identification of major genes conferring resistance to Plum Pox Virus (PPV)
auteur
Sabrina Micali, J. Giovinazzi, M.T. Dettorri, L. Ferretti, E. Vendramin, R. Quarta, G. Pasquini, Thierry Pascal, E. Giordani, M. Barba, Ignazio Verde
article
7. International Peach Symposium, Jun 2009, Lleida, Spain
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titre
Peach fruit quality : candidate genes or map-based cloning strategy ?
auteur
Elisabeth Dirlewanger, Karima Boudehri, Christel Renaud, Camille Croset, Loick Le Dantec, E. Illa, Patrick Lambert, Catherine Deborde, Mickael M. Maucourt, Bénédicte Quilot-Turion, W. Howad, Annick Moing, Jean-Luc Poessel, Abdelhafid A. Bendahmane, Pere Arus
article
7. International Peach Symposium, Jun 2009, Lleida, Spain. 1 p
typdoc
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titre
Approche populationnelle de la durabilité de la résistance au puceron Aphis gossypii chez le melon : Projet Aptipuce
auteur
Nathalie Boissot
article
2. Journées du réseau Biologie et Adaptation des Pucerons et Organismes Associés (BAPOA), Réseau Biologie Adaptative des Pucerons et des Organismes Associés (BAPOA). FRA., Jun 2009, Paris, France
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titre
The AGRI GEN RES SAFENUT: establishing hazelnut and almond core collections on service of rationalization of national ex-situ collections
auteur
A. Solar, M. Rovira, Henri Duval, L. Bacchetta, D. Avanzato, R. Botta, P. Drogoudi, I Metzidakis, Jean Paul Sarraquigne, A.P. Silva, R. Socias, D. Spera
article
19. EUCARPIA Conference, May 2009, Ljubljana, Slovenia
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titre
Re-evaluation of the Globodera pallida resistance QTLs originating from the wild potato species Solanum spegazzinii
auteur
Bernard Caromel, Marie-Claire Kerlan, Sarah Danan, Didier Mugniery, Véronique Lefebvre
article
3. Annual Meeting, COST 872 Workshop & MC, May 2009, Toledo, Spain
typdoc
Conference papers
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https://hal.inrae.fr/hal-02751896/file/35856_20100226014042126_1.pdf BibTex
titre
Genetic and physiological determinants controlling cadmium accumulation in lettuce (Lactuca sativa)
auteur
Walid Zorrig, Catherine Sarrobert, Hatem Rouached, Brigitte B. Maisonneuve, Jean-Claude Davidian, Chedly Abdelly, Pierre Berthomieu
article
Workshop of WG 1 and WG2 on Uptake, sequestration and detoxification An integrated approach, COST action 859, Apr 2009, Szeged, Hungary
typdoc
Conference papers
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titre
Late blight quantitative resistance in potato diversity: QTL meta-analysis including the novel resistance sources Solanum sparsipilum and Solanum spegazzinii
auteur
Véronique Lefebvre, Sarah Danan, Jean-Baptiste Veyrieras, Bernard Caromel, Sophie Ewert, Jean-Eric Chauvin
article
BioExploit Science Meeting 2009, Mar 2009, Wageningen, Netherlands
typdoc
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titre
Analysis of the almond genetic diversity by SSR molecular markers - genotyping of the S-incompatibility alleles
auteur
Henri Duval, Patrick Samper, Patrick Lambert
article
2. Annual meeting of SAFENUT Project, Mar 2009, Vila Real, Portugal
typdoc
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titre
Integrative approach of size and composition of tomato fruit combining ecophysiology, genetic, genomic and metabolomic analysis
auteur
Marion Prudent, Nadia Bertin, - Phuc Thi Do, Pasquale Tripodi, Michel M. Génard, Alisdair Fernie, Silvana Grandillo, Mathilde M. Causse
article
Naro International Symposium Solanaceae, Mar 2009, Tsu, Mie, Japan
typdoc
Conference papers
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https://hal.inrae.fr/hal-02753522/file/27873_20090513025220475_1.pdf BibTex
titre
TILLING in tomato: a reverse genetic approach for tomato crop improvement. The french platforms
auteur
Florence Piron, Maryse Nicolaï, Christophe Rothan, Dani Zamir, Carole Caranta, Abdelhafid A. Bendahmane, Mathilde M. Causse
article
Naro International Symposium Solanaceae, Mar 2009, Tsu, Mie, Japan
typdoc
Conference papers
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https://hal.inrae.fr/hal-02754219/file/30892_20090513023207952_1.pdf BibTex
titre
Spatial upscaling of on-farm indicators: Concepts to use a scientific knowledge base to match policymakers' requirements
auteur
Nadine Turpin, Olivier Therond, Sophie Ewert, I. Bezlepkina, J. Alkan Olsson, Christian C. Bockstaller, S. Janssen
article
Integrated Assessment of Agriculture and Sustainable Development (AgSAP) : Setting the Agenda for Science and Policy, Mar 2009, Egmond aan Zee, Netherlands
typdoc
Conference papers
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https://hal.science/hal-01195278/file/38220_20100226110116410_1.pdf https://hal.science/hal-01195278/file/38220_20110225120002619_2.pdf BibTex
titre
Optimisation de la méthode VIGS chez le piment (Virus Induced Gene Silencing).
auteur
Jawad Aarrouf, Véronique Lefebvre
article
Journée des sélectionneurs, GAFL/INRA Avignon, Feb 2009, Avignon, France
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titre
ResistVir : co-ordination of research on genetic resistance to plant pathogenic viruses, and their vectors, in european crops
auteur
Florence Poey, Carole Caranta, Juan J. Lopez-Moya, Miguel Aranda, Mark M. Tepfer, Olivier Le Gall, Andy Maule, Margrit Laimer, Mike Adcock, Michael Wassenegger
article
12. Rencontres de Virologie Végétale, Jan 2009, Aussois, France
typdoc
Conference papers
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https://hal.inrae.fr/hal-02756226/file/26214_20090414033332255_1.pdf BibTex
titre
Rôle des facteurs d’initiation de la traduction dans l’accumulation des virus de la famille des Luteoviridae
auteur
Catherine Reinbold, Véronique Ziegler-Graff, Carole Caranta, Véronique Brault
article
12. Rencontre de Virologie Végétale, Jan 2009, Aussois, France
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titre
TILLING in tomato: a reverse genetic approach for functional analysis of eIF4E-mediated resistance and extension of the set of resistance alleles for breeding
auteur
Maryse Nicolaï, Aurélie Salgues, André Moretti, Florence Piron, Abdelhafid A. Bendahmane, Jean-Luc J.-L. Gallois, Carole Caranta
article
12. Rencontres de Virologie Végétale, Jan 2009, Aussois, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02751340/file/31872_20090415112450457_1.pdf BibTex
titre
Natural variation and functional analyses provide evidence for coevolution between plant eIF4E and potyviral VPg
auteur
Carine Charron, Maryse Nicolaï, Jean-Luc J.-L. Gallois, Pascale Sanchez, Christophe Robaglia, Benoît Moury, Alain Palloix, Carole Caranta
article
12. Rencontres de Virologie Végétale, Jan 2009, Aussois, France
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Conference papers
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https://hal.inrae.fr/hal-02751888/file/28601_20090415103149927_1.pdf BibTex
titre
Determination of fruit quality using Fourier-Tranform infrared spectroscopy : example of apricot and tomato
auteur
Jean Marc Audergon, Dominique D. Bertrand, Sylvie Bureau, Mathilde M. Causse, Alain Lecomte, Maryse Reich, Catherine M.G.C. Renard, David Ruiz, Iwona Scibisz
article
5. Rencontres du Végétal, Jan 2009, Angers, France
typdoc
Conference papers
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02757691/file/31667_20090410030623858_1.pdf BibTex
titre
Impact of fruit and leaf irradiance on tomato fruit content in sugars and ascorbic acid
auteur
Hélène Gautier, Michel M. Génard, Capucine Massot, Rebecca Stevens
article
5. Rencontres du Végétal, Jan 2009, Angers, France
typdoc
Conference papers
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https://hal.inrae.fr/hal-02757242/file/31736_20090410101915986_1.pdf BibTex
titre
The genetic control of organoleptic quality of tomato fruit: prospects for breeding
auteur
Mathilde M. Causse
article
5. Rencontres du Végétal, Jan 2009, Angers, France
typdoc
Conference papers
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https://hal.inrae.fr/hal-02756358/file/28132_20090410092332465_1.pdf BibTex
titre
Linkage disequilibrium mapping for tomato fruit quality traits
auteur
Nicolas N. Ranc, Marie-Christine Le Paslier, Stephane Munos, Sophie Rolland, Esther Pelpoir, Jean-Paul Bouchet, Sylvain S. Santoni, Dominique D. Brunel, Mathilde M. Causse
article
17. Plant & Animal Genomes Conference, Jan 2009, San Diego, United States
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titre
High throughput SNPs discovery in grapevine, poplar and tomato
auteur
Marie-Christine Le Paslier, Rémi Bounon, Aurelie A. Chauveau, Vincent Thareau, Aurelie A. Canaguier, Isabelle Clainche, Cléa Houel, Marco M. Moroldo, Sophie Rolland, Nicolas N. Ranc, Alois Bresson, Isabella Paolucci
article
17. Plant & Animal Genomes conference, Jan 2009, San Diego, United States
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Master thesis

titre
Identification du (des) gène(s) impliqué(s) dans le phénotype peu de fructose d’une population interspécifique de pêchers (Prunus persica)
auteur
Mathilde Hoerter
article
Sciences du Vivant [q-bio]. 2009
resume
A lack of fructose was observed for some individuals in an interspécific population of peaches. Fructose is the sweetest sugar and the most important in fruit flavour. We are looking for the origin of this lack of fructose (in less than ¼ of individuals) which is under the control of a major gene mapped on the first linkage group of the peach genome. The candidate gene approach was chosen in order to identify this gene. Functional candidates were selected from the fructose metabolism and then sequences extracted from Prunus databases. We also used microsynteny to find positional candidate genes. Sorbitol dehydrogenase (SDH) was the best candidate as it is responsible for fructose synthesis. However, SDH appeared no longer a candidate since it was mapped on linkage group 2. SDH gene is overexpressed in the peach genotypes with a lack of fructose, and the enzyme activity level is also higher in those genotypes. Accordingly, SDH is not responsible for the lack of fructose but may be upper regulated to counterweight this lack. The lack of fructose may be the consequence of the dysfunction of an enzyme involved in the degradation or transformation of fructose, such as fructokinases. A fructokinase (FRK-b), located on the first linkage group, near the locus of interest controlling fructose/glucose ratio, appeared a good candidate both for position and function.We found no difference between the parental sequences of the partial protein of FRK-b. A partial sequence of a Phosphoglucose isomerase (PGI) was cloned. This cloning allowed us identifying polymorphism between parents. This polymorphism is in charge of changes in the secondary structure of PGI. In order to determine if those modifications are responsible for some changes in the enzyme functionality, expression and activity levels have to be estimated between the individuals “with” and “without” fructose.
typdoc
Master thesis
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02819724/file/47645_20110422112922321_1.pdf BibTex

Poster communications

titre
Providing genomic tools to increase the efficiency of marker assisted selection of complex traits
auteur
Maryse Nicolaï, Kleovoulos Athanasiou, Anne-Marie Sage-Palloix, Ghislaine Nemouchi, Bruno Savio, Therese Phaly, Sébastien Cali, Valérie Pflieger, Nurudeen Adeniyi Alimi, Véronique Lefebvre, Marnik Vuylstecke, Alain Palloix
article
European Workshop: Genomics for research and molecular diagnostics, Oct 2009, Milan, Italy. 1 p., 2009
typdoc
Poster communications
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titre
Etude des populations de Bremia en Provence
auteur
Brigitte Maisonneuve, Salima Kherchache
article
Salon Méditerranéen Interprofessionnel de la. Filière des Fruits et Légumes (MIFFEL) 2009, Oct 2009, Avignon, France. 2009
typdoc
Poster communications
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titre
Diversité génétique de Bremia lactucae (Regel), agent du mildiou de la laitue, Lactuca sativa
auteur
Romain R. Valade, Brigitte B. Maisonneuve, Claire C. Neema
article
31. Réunion du Groupe de Biologie et Génétique des Populations, Aug 2009, Grenoble, France. , 2009, Le Petit Pois Déridé 2009
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02753390/file/39780_20100609033322919_1.pdf BibTex
titre
Genetic and physiological determinants controlling cadmium accumulation in lettuce (Lactuca sativa)
auteur
Walid Zorrig, C. Sarrobert, Aïda Rouached, Brigitte B. Maisonneuve, Jean-Claude Davidian, Chedly Abdelly, Pierre Berthomieu
article
8. Colloque National de la SFBV, Jul 2009, Strasbourg, France. 2009, 8ème Colloque National de la SFBV8th SFBV Meeting
typdoc
Poster communications
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02755969/file/36712_20100611015943979_1.pdf BibTex
titre
QTL anlysis of characters involved in fruit quality in Prunus persica and a related species and co-location with candidate genes in the European Project ISAFRUIT
auteur
Elisabeth Dirlewanger, Karima Boudehri, Christel Renaud, Mathieu Fouché, Camille Croset, Loick Le Dantec, E. Illa, Patrick Lambert, Catherine Deborde, Mickael M. Maucourt, Bénédicte Quilot-Turion, W. Howad, Annick Moing, Jean-Luc Poessel, Pere Arus
article
7. International Peach Symposium, Jun 2009, Lleida, Spain. 2 p., 2009
typdoc
Poster communications
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titre
Genetic and physiological determinants controlling cadmium accumulation in lettuce (Lactuca sativa)
auteur
Walid Zorrig, Catherine Sarrobert, Aïda Rouached, Brigitte B. Maisonneuve, Jean-Claude Davidian, Chedly Abdelly, Pierre Berthomieu
article
COST action 859 Uptake, sequestration and detoxification - An integrated approach, Apr 2009, Szeged, Hungary. 2009, COST Action 859Abstract book
typdoc
Poster communications
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titre
Under- or over-expression of some eukaryotic translation initiation factor alleles in transgenic tomato and lettuce : effect on virus infection
auteur
Marianne Mazier, Raquel Sanjuan, Fabrice Flamain, Verane Sarnette, Emmanuel Botton, Sylvie S. German-Retana, André Moretti, Benoît Moury, Bérenger Janzac, Aline Le Menn, Patrick P. Rousselle, Brigitte B. Maisonneuve, Carole Caranta
article
12. Rencontres de virologie végétale, Jan 2009, Aussois, France. 2009, Rencontres de virologie végétale
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Poster communications
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Reports

titre
Allelic diversity study of the S-RNase gene in almond
auteur
Ludovic Alaux
article
[Stage] Université d'Avignon et des Pays de Vaucluse (UAPV), Avignon, FRA. 2009, 19 p. + 9 p. annexes
resume
Almond (Prunus dulcis) has a gametophytic pollen self-incompatibility. This locus contains the S-RNase gene and the SFB gene. The aim of this study is to determine the alleles of the S-RNase gene in the Avignon INRA almond genetic resource collection. This study is in the SAFENUT project. We used PCR techniques to detect the size polymorphism of the S-RNase gene introns and determine S alleles. These genotypes are confirmed with allele-specific primers. We also sequenced the genotypes which results differed from known alleles. During this study, we genotyped 224 cultivars and found several new alleles. We noticed that the S-RNase gene has an important diversity. Indeed, a lot of different alleles of this gene exist, and these ones are associated with sequences very variable.
typdoc
Reports
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02824380/file/44599_20111114111431704_1.pdf BibTex
titre
Durabilité de la résistance du melon à A. gossypii
auteur
Nathalie Boissot
article
[Contrat] 2009
resume
La protection des cultures maraîchères contre les insectes phytophages passe traditionnellement par l’utilisation de pesticides. L’utilisation de variétés résistantes permet une production plus respectueuse de l’environnement et du consommateur. Chez le melon, il existe une résistance à la colonisation par le puceron Aphis gossypii, ravageur majeur des Cucurbitacées. Cette résistance, conférée par un gène nommé Vat, est largement utilisée dans les variétés cultivées ; elle constitue un modèle économiquement intéressant. La résistance exerce désormais une pression de sélection sur les populations d’A. gossypii à une échelle régionale. On peut craindre, de la même manière que des populations de pucerons résistantes aux insecticides ont été sélectionnées par l’usage intensif de certaines molécules actives, le développement de pucerons capables de surmonter la résistance conférée par Vat. En effet, depuis quelques années, des foyers d’A. gossypii capables de se développer sur des variétés de melon porteuses du gène Vat ont été observés sur le terrain. Le projet a pour objectif de préciser les stratégies d'utilisation de la résistance conférée par Vat afin de lutter contre son contournement, en y associant des gènes de résistance partiels (appelés QTLs) autres que Vat. Dans le cadre du projet, des génotypes de melon associant le gène Vat et des QTLs seront construits puis mis en essai de plein champ dans différents bassins de production pour mesurer la pression de sélection qu’ils exercent sur les populations d’A. gossypii.
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https://hal.inrae.fr/hal-02815516/file/Durabilit%C3%A9%20de%20la%20r%C3%A9sistance%20du%20melon%20%C3%A0%20A_1.%20gossypii BibTex
titre
Amélioration des Hôtes différentiels Bremia : obtention de lignées de laitue à résistance monogénique : Rapport de fin de contrat CTPS-CASDAR C06-08
auteur
Brigitte Maisonneuve
article
[Contrat] Enza Zaden; Gautier Semence; Rijk Zwaan; Seminis Vegetable Seeds; Syngenta France. 2009
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Ecophyto R&D. Vers des systèmes de culture économes en produits phytosanitaires, Volet 1 . Tome V. Analyse comparative de différents systèmes en cultures légumières.
auteur
Eva Brismontier, Philippe C. Nicot, Michel Pitrat, Dominique Blancard, Frederique F. Bressoud, Bernard Le Delliou, Catherine Mazollier, Mireille Navarrete, Gérard Roche, Catherine Taussig, Marc Tchamitchian, Yannie Trottin-Caudal, François Villeneuve, Gilles Wuster, Pierre Stengel, Laurent Lapchin, Charles-Antoine Dedryver, Therese Volay
article
[Rapport de recherche] INRA; Ecophyto. 2009
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https://hal.inrae.fr/hal-02822559/file/34132_20100921040645390_1.pdf BibTex
titre
Ecophyto R&D. Vers des systèmes de culture économes en pesticides. Volet 1. Tome I : méthodologie générale
auteur
Alain Carpentier, Charles-Antoine Dedryver, Raymond Reau, Therese Volay, Jean-Pierre Butault, Henri Darmency, Jean Marc Barbier, Philippe Debaeke, Marc Delos, Christian Gary, Philippe Girardin, Laurence Guichard, Jean-Marc Meynard, Philippe C. Nicot, Michel Pitrat, Bernard Rolland, Benoit Sauphanor, Philippe Viaux, Christian Walter
article
[Rapport de recherche] INRA; Ecophyto. 2009, 35 p. + ann
resume
The first report of the program ECOPHYTO R & D brings together the general principles of methods that are employed by expert groups, first of all, in various stages of the comparative study : techno-economic status of pesticide reduction (part 1) and secondly, the noted stage of setting up a network of data acquisition references (part 2)
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https://hal.science/hal-01186931/file/33397_20100715032114856_1.pdf BibTex

2008

Conference papers

titre
Étude des déterminismes physiologiques et génétiques contrôlant l'accumulation du cadmium chez la laitue (Lactuca sativa)
auteur
Walid Zorrig, Hatem Rouached, Brigitte Maisonneuve, Chedly Abdelly, P. Berthomieu
article
7. journées biotechnologiques de l'Association Tunisienne de Biotechnologie, Dec 2008, Hammamet, Tunisie
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https://hal.inrae.fr/hal-02816413/file/ZORRIG_communication_2008_1.pdf BibTex
titre
Constraints on evolution of virus avirulence factors predict the durability of corresponding plant resistances
auteur
Bérenger Janzac, Frederic Fabre, Alain Palloix, Benoît Moury
article
International Conference, Nov 2008, Cadiz, Spain
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titre
Translation initiation factors: kingpin in plant resistance to RNA viruses
auteur
Carole Caranta
article
International Conference, Nov 2008, Cadiz, Spain
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titre
Aphid and virus resistance triggered by the CC-NBS-LRR Vat melon gene
auteur
Catherine Dogimont, Nathalie Boissot, Veronique Chovelon, Sarah Tual, Xavier Grand, Vincent Rittener-Ruff, Nathalie Giovinazzo, Abdelhafid A. Bendahmane
article
International Conference, Nov 2008, Cadiz, Spain
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Peach breeding for multiple resistances to pests and diseases contributes to integrated fruit production
auteur
Thierry Pascal, Patrick Lambert, Jean-Luc Poessel, Véronique Decroocq, Marie-Hélène Sauge
article
7 International Conference on Integrated Fruit Production, Oct 2008, Avignon, France
resume
In spite of the worldwide decline in peach production, a constant stream of new varieties are being provide to fruit growers. For the greater part most of these new varieties being produced by private peach breeders, and as a consequence very few are selected on the basis of their resistance to pests or diseases, while the demand of consumers continues to be directed towards a quality fruit product which is free of pesticide residues. Within the framework of one INRA multidisciplinary group (Avignon-Bordeaux), we have developed for several years a wide applied breeding program aiming at improving the resistance of the peach tree to three of its main enemies: the green peach aphid (Myzus persicae), peach powdery mildew (Sphaerotheca pannosa var. persicae) and sharka disease (Plum Pox Virus). This work is globally conducted in a research context oriented towards varietal innovation including fruit quality and durable resistances building. In this way, two complementary approaches have been preferentially held for respectively improving the genetic gain by time unit and a better understanding of peach-enemies relationships. First, a genetic approach integrating the quantitative trait loci or major genes mapping for the development of molecular assisted selection. Second, a functional approach (i) leaning on the study of the insect behaviour and the plant metabolites involved in the resistance to M. persicae (ii) coupled to a candidate-genes research mainly developped for PPV resistance. Whole of first results and perspectives are discussed.
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Proteomics to characterize the effect of a tomato genomic region on the fruit sensitivity to cold storage
auteur
David Page, Barbara Gouble, Caroline Callot, Andre Kretzschmar, Benoit B. Valot, Mathilde M. Causse, Mireille Faurobert
article
Proteomlux 2008, Proteomics in plants, microorganisms and environment, Oct 2008, Luxembourg, Luxembourg
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titre
Consumer preferences for tomato cultivars in Europe: a comparison over three countries
auteur
S. Grandillo, N. Holthuysen, Marina Lee, B. Navez, Fiorella Sinesio, Mathilde M. Causse
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02754588/file/22767_20081103052031264_1.pdf BibTex
titre
Linkage disequilibrium mapping for tomato fruit quality traits
auteur
Mathilde M. Causse, Nicolas N. Ranc, Stephane Munos, Sophie Rolland, Marie-Christine Le Paslier, Sylvain S. Santoni, Jean-Paul Bouchet, Dominique D. Brunel
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02751766/file/25797_20081104085439913_1.pdf BibTex
titre
Phytophthora - pepper interaction: genetic, cytological and biochemical analyses
auteur
Véronique Lefebvre, Stephanie Mallard, Sarah Danan, Julien Bonnet, Arnaud Thabuis, Jawad Aarrouf, Anne-Marie Sage-Palloix, Patrick Signoret, Alain Palloix
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02756760/file/21241_20081103051417393_1.pdf BibTex
titre
Exploration of late blight quantitative resistance in potato diversity: QTL meta-analysis including the novel resistance sources Solanum sparsiplum and Solanum spegazzinii
auteur
Sarah Danan, Jean-Eric Chauvin, Bernard Caromel, Jean-Baptiste Veyrieras, Jean-Paul Dantec, Roland Pellé, Nadia Lama, Véronique Lefebvre
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02757139/file/21848_20081103053915195_1.pdf BibTex
titre
Molecular mapping in Capsicum annuum of the tomato Ve2 gene
auteur
Lorenzo Barchi, E. Portis, Véronique Lefebvre, Alain Palloix, S. Lanteri
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02757830/file/19423_20081103040013867_1.pdf BibTex
titre
Integrative approach of size and composition of tomato fruit combining ecophysiology, genetic, genomic and metabolic analysis
auteur
Mathilde M. Causse, Marion Prudent, Nadia Bertin, P. Thi Do, P. Tripodi, Michel M. Génard, A. Fernie, S. Grandillo
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02754010/file/25168_20081103050334102_1.pdf BibTex
titre
Is Phy-P5 a major broad-spectrum resistance QTL conferring resistance to Phytophthora in Solanaceae ?
auteur
Stephanie Mallard, Sophie Ewert, David Tricon, Caroline Callot, Morgane Ardisson, Véronique Lefebvre
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02752745/file/20658_20081103040615839_1.pdf BibTex
titre
The combination of resistance alleles at a major and minor effect QTLs confers a broad spectrum resistance to Globodera pallida
auteur
Bernard Caromel, Marie-Claire Kerlan, Jawad Aarrouf, Claudia Rouaux, Nadia Lama, Jean-Paul Dantec, Didier Mugniery, Véronique Lefebvre
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02757105/file/22351_20081103051212030_1.pdf BibTex
titre
Development and evaluation of functional markers for tomato DUS testing of disease resistance genes
auteur
Carole Caranta, P. Arens, Laetitia Cavellini, Sophie Rolland, André Moretti, C. Mansilla, D. Deinum, D. Calvache, F. Ponz, C. Collonier, D. Smilde, Rene Mathis, B. Vosman
article
5. Solanaceae Genome Worshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02755619/file/21699_20081103045823552_1.pdf BibTex
titre
Natural variation and functional analysis provide evidence for co-evolution between pepper eIF4E and potyviral Vpg
auteur
Carole Caranta, Carine Charron, Maryse Nicolaï, Jean-Luc J.-L. Gallois, Alain Palloix, Christophe Robaglia, Stéphane de Mita, Jacques David
article
5. Solanaceae Genome Workshop, Oct 2008, Cologne, Germany
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https://hal.inrae.fr/hal-02757223/file/24902_20081103054243791_1.pdf BibTex
titre
Isolation and molecular mapping in Capsicum annuum of the tomato Ve2 gene conferring resistance to Verticillium spp.
auteur
Lorenzo Barchi, E. Portis, Véronique Lefebvre, Alain Palloix, S. Lanteri
article
52. Italian Society of Agricultural Genetics Annual Congress, Sep 2008, Padova, Italy
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The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development
auteur
Jean-Luc Gallois, Anouchka Debast Guyon, Alain Lecureuil, Daniel Vezon, V Carpentier, Mathilde Grelon, Sandrine Bonhomme, Philippe Guerche
article
Annual Meeting of the Society-for-Experimental-Biology, Jul 2008, Marseille, France
resume
The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development. Annual Meeting of the Society-for-Experimental-Biology
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Role of the UPS pathway in sexual reproduction: Screening for Arabidopsis gametophytic mutations affecting F-box protein genes
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Sandrine Bonhomme, Monique Durandet, Anouchka Debast Guyon, Alain Lecureuil, Jean-Luc Gallois, Philippe Guerche
article
Annual Main Meeting of the Society for Experimental Biology, Jul 2008, Marseille, France. ⟨10.1016/j.cbpa.2008.04.473⟩
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absent
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https://hal.science/hal-01203913/file/45482_20110428023742804_1.pdf BibTex
titre
Major-effect late blight resistance QTLs in Solanum sparsipilum and Solanum spegazzinii map in resistance gene clusters of chromosomes VIII and X
auteur
Sarah Danan, Jean-Eric Chauvin, Véronique Lefebvre
article
PhD Summer school, Jun 2008, Wageningen, Netherlands
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Anchorage of the genomic region harbouring a major effect QTL for resistance to Phytophthora capsici in pepper to the tomato and potato genomes
auteur
Stephanie Mallard
article
PhD summer school, Jun 2008, Wageningen, Netherlands
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From the detection of disease resistance loci in Solanaceae to their comparative genetics and molecular basis
auteur
Véronique Lefebvre
article
PhD Summer School, Jun 2008, Wageningen, Netherlands
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Evolution of apricot fruit quality attributes of the released cultivars over the last 50 years
auteur
David Tricon, David Ruiz, Hedia Bourguiba, Alain Blanc, Sylvie Bureau, Barbara Gouble, Maryse Reich, Catherine M.G.C. Renard, Guy Clauzel, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Among fruit Species, apricot (Prunus armeniaca L.) is characterized by a large phenotypic diversity due to its geographical origins and phylogenetic structures. At the same time, apricot production is deeply evolving under the pressure of breeding programmes. Are cent increase of new registered cultivars has raised a strong interest in apricot, especially in Europe. Currently, modifications on fruit quality attributes have been observed. To estimate its incidence, 237 cultivars registered between 1950 and 2006 were analysed. Representative fruit samples collecte d at the maturity stage in collection or chards were observed from 2003 to 2007. Harvest period and fruit quality attributes have been characterized: fruit weight, fruit firmness, ground and colours, soluble solids content and titratable acidity. The incidence of genetic and geographic origins on fruit quality attributes were evaluated according to there gistration period. France and the United States were the mostimportant cultivar providers in the registration process. An enlargement of the genetic basis of the breeding stocks was observed with the use of new parents such as 'Goldrich' and 'Oranged'. An extension of the harvest scas on has been recenty observed. Significant increases in fruit weight and blush colourc all be explained by the use of new parents; while no significant variations on fruit firmness, soluble solids content and titratable acidity were observed due to the enlargement of the variability in the last registered cultivars.
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Evolution of apricot fruit quality attributes of the released cultivars over the last 35 years
auteur
David Tricon, David Ruiz, Hedia Bourguiba, Alain Blanc, Barbara Gouble, Marie Grotte, Catherine M.G.C. Renard, Maryse Reich, Sylvie Bureau, Guy Clauzel, Richard Brand, S. Semon, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
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New recent selections issued from INRA's apricot breeding program
auteur
Jean Marc Audergon, Alain Blanc, Frederic Gilles, Guy Clauzel, J.M. Broquaire, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, C. Pitiot
article
14. International Symposium, Jun 2008, Matera, Italy
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Identification of QTLs for fruit quality traits in apricot
auteur
David Ruiz, Patrick Lambert, Jean Marc Audergon, L. Dondini, Stefano Tartarini, M. Adami, F. Gennari, C. Cervellati, P. de Franceschi, S. Sansavini, Sylvie Bureau, Barbara Gouble, Maryse Reich, Catherine M.G.C. Renard, Daniele Bassi, R. Testolin
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Fruit quality is one of the main bottlenecks for apricot consumers. Despite the relevance of phenotyping during the field evaluation phase in breeding programmes, the molecular bases of fruit quality have been poorly investigated. The identitication of genomic regions involved in fruit quality traits can be pursued by joining the phenotypic data with a molecular-based approach. The related QTL definition offers the opportunity to optimize the apricot breeding programmes by introducing an early marker assisted selection (MAS), and identify candidate genes related to these traits. These are the main ohjectives of the ISAFRUIT WP6.I European IP Project concerning peach, apricot and apple fruits. On apricot, INRA UGAFL Avignon (Fr) and DCA, University of Bologna (It) were in charge of the characterization of two apricot populations: 'Goldrich' x 'Moniqui' and 'Lito x B081604311' (of about 120 seedlings each). Both progenies have been characterized over two consecutive years for several physical and chemical characters (blooming and maturity dates, fresh weight, firmness, colour, dry matter, kernel weight, flesh adhesion, acid and sugar contents). Genetic linkage maps of each population were constructed by SSRs markers and QTLs analyses were performed for all the investigated quality traits. Several QTLs, distributed in all the linkage groups, were detected in both populations for all the analyzed traits except fruit firmness. A good QTL stahility was observed between years and this offers an interesting opportunity to use the related markers in an MAS process.
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Comparative analysis of tunisian apricot cultivars based on morphological and molecular markers
auteur
Lamia Krichen, N. Trifi-Farah, M. Marrakchi, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Tunisian apricot genetic resources, formerly cultivated in different geographical areas of the country in the North (Testour and Ras Jbel), the Centre (Kairouan, Sfax, Mahdia) and the South (Gabes, Mareth, Jerba, Gafsa, Midess), cover different climatic conditions ranging from the sub-humid (North) to the arid (South) areas. Their phenotypic diversity is rather large, even if only a few cultivars are of market interest. For this reason, apricot landraces are threatened by severe genetic erosion. To face the problem and preserve the existing biodiversity, actions have been carried on and 48 distinct cultivars have been identitied and characterized on morphological and molecular bases. The present study deals with the comparative analysis of the characterization data sets including the relationship between molecular markers and morphological traits, and with the assessment of the phylogenetic structure of the related cultivars with regard to their geographical origin. Morphological charactterization has been assessed using 28 qualitative characters according to the UPOV descriptor and 13 quantitative morphometric parameters. AFLP molecular markers were investigated using 7 primer combinations: 268 polymorphic bands were registered suggesting a high level of polymorphism. High genetic diversity is revealed among the Tunisian apricot cultivars. The 48 cultivars were distinguishable either by morphological or by molecular bases. They were structured into two groups genetically related to two main introductions of apricot in Tunisia. Diversity structure seems to be correlated to tbe geographical distribution of the accessions where the three Northern, Central and Southern regions are clearly distinguished.
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Apricot genetic resource management. New prospects offered by phylogeographic and association genetic approaches. Application in the mediterranean basin
auteur
Hedia Bourguiba, Lamia Krichen, Neïla Trifi-Farah, Bouchaib Khadari, Sylvain S. Santoni, Mohamed Marrakchi, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
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Rubisco (r) a new french line of apricot
auteur
Jean Marc Audergon, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, Alain Blanc, Guy Clauzel, C. Pitiot
article
14. International Symposium, Jun 2008, Matera, Italy
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Apricot genetic resource management. New prospects offered by phylo-geographic and association genetic approaches. Application in the mediterranean basin germplasm
auteur
Hedia Bourguiba, Lamia Krichen, Neïla Trifi-Farah, Bouchaib Khadari, Sylvain S. Santoni, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Apricot (Prunus armeniaca L.) was introduced from China to the Mediterranean region through at least two majn routes and a large number of cultivars are here developed in the different countries. In order to characterize the actual Mediterranean genetic variability, 251 apricot accessions from France, Spain, Tunisia, and Turkey were investigated with a common set of markers. Twenty five SSR loci, covering the whole Prunus genome were chosen according to their expected polymorphism. Our results confirmed that SSR markers are efticient tools for fingerprinting cultivars and for determining the genetic structure of apricot Mediterranean populations as well. On the base of a Bayesian analysis four main phylogeograpbical groups have been identified: Tunisian, Enropean (Mediterranean & continental), Turkish and a diversification group joining accessions from central Asia to Europe.
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Carotenoid composition in apricot fruits: a preliminary investigation on three italian cultivars
auteur
Annamaria Leccese, Susanna Bartolini, Sylvie Bureau, Maryse Reich, Catherine M.G.C. Renard, Jean Marc Audergon, Raffaella Viti
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Recently, there has been increased interest in the carotenoid composition of fruits as they can have nutritional interest beyond the pro-vitamin A molecules (ά and βcarotene, β-cryptoxanthin). Among fruits, apricot species is known to be a rich source of carotenoids, responsible for fruit colour. The aim of this work was to analyze carotenoid composition at harvest and after cold storage in three apricot cultivars: 'Cafona', 'Pellecchiella' and 'Dulcinea'. Fruits were characterized for physical and chemical properties and carotenoid content at harvest and after 7 and 14 days at +4°C. Carotenoid compounds were extracted in methanol/hexane from fruit powder and analysed by HPLC-DAD. ' Pellecchiella' showed the highest β-carotene level, while 'Cafona ' and ' Dulcinca' showed similar lower b-carotene level. Despite this similarity, phytoene and phytofluene were 1.6 and 1.3 fold respectively higher in 'Cafona' than in ' Dulcinea'. In all the three cultivars, 7 days of cold storage at +4°C did not particularly affect carotenoid content. After 14 days in both Vesuvian cultivars, the three carotenes increased, while in 'Dulcinea' a decrease in phytofluene and β-carotene was registered. As regards nutritional evaluation of apricot fruit in terms of carotenoid content, it is highly recommended to make general conclusions with respect to the cultivars-specific results. Changes in β-carotene content after storage probably depend on the cultivar.
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Determination of apricot fruit quality using Fourier-transform infrared spectroscopy
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Sylvie Bureau, Maryse Reich, Barbara Gouble, Catherine M.G.C. Renard, Dominique D. Bertrand, David Ruiz, Jean Marc Audergon, Alain Lecomte
article
14. International Symposium, Jun 2008, Matera, Italy
resume
This work reports the rapid determination of apricot fruit quality traits using Fouder Transform near ( IR) and mid (MlR) infrared reflectance spectroscopy. The FT-NIR (800-2500 nm) spectra were acquired on intact fruits using an integrating sphere (diffuse reflectance). The FT-MlR spectra were acquired on fruit slurries using a horizontal ATR cell (Attenuated Total Reflectance). Relationships between spectral data and quality attributes obtained by traditional methods are evaluated by the application of chemometric techniques based on partial least squares (PLS) on fruit set divided randomly into two groups: 598 fruits for calibration and 279 for validation. The non-destructive method using NIR spectroscopy allows the prediction of soluble solids content and titratable acidity (correlation coefficient of 0.92 and 0.89, respectively, and root mean square error of prediction of 0.98% Brix and 3.62 meq.100g-1FW, respectively). The ATR-FTIR performed on apricot slurries is well adapted for the prediction of the individual compounds: sucrose, glucose, malic and citric acids (correlation coefficients greater than or equal to 0.92 and errors smaller than 16%). Prediction model for fructose, the minor compound in apricot, showed the lowest performance with r = 0.86 and error of validation of 18%. Moreover, the technique that consists in acquiring spectra and performing PCA on spectra allows the discrimination of apricot sets according to their ripening stages and their genetic characteristics.
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Apricot genetic resource management. New prospects offered by phylo-geographic and association genetic approaches. Application in the mediterranean basin germplasm
auteur
Hedia Bourguiba, Lamia Krichen, Neïla Trifi-Farah, Bouchaib Khadari, Sylvain S. Santoni, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Apricot (Prunus armeniaca L.) was introduced from China to the Mediterranean region through at least two majn routes and a large number of cultivars are here developed in the different countries. In order to characterize the actual Mediterranean genetic variability, 251 apricot accessions from France, Spain, Tunisia, and Turkey were investigated with a common set of markers. Twenty five SSR loci, covering the whole Prunus genome were chosen according to their expected polymorphism. Our results confirmed that SSR markers are efticient tools for fingerprinting cultivars and for determining the genetic structure of apricot Mediterranean populations as well. On the base of a Bayesian analysis four main phylogeograpbical groups have been identified: Tunisian, Enropean (Mediterranean & continental), Turkish and a diversification group joining accessions from central Asia to Europe.
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ISAFRUIT IP project. Genetic and molecular bases of peach and apricot fruit quality
auteur
Jean Marc Audergon, Alexandre Bachellez, Alain Blanc, Marielle Boge, K. Boudheri, Sylvie Bureau, Marie Noëlle Corre, Camille Croset, Catherine Deborde, Elisabeth Dirlewanger, L. Dondini, Anne Marie Ferréol, Barbara Gouble, Marie Grotte, Patrick Lambert, Mickael M. Maucourt, Annick Moing, Sarah Monllor, Thierry Pascal, Jean-Luc Poessel, Bénédicte Quilot, Maryse Reich, Patrice Reling, Christel Renaud, David Ruiz, Veronique Signoret, Pere Arus
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14. International Symposium, Jun 2008, Matera, Italy
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Rubisco ® a new french series of apricot
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Jean Marc Audergon, Alain Blanc, Guy Clauzel, C. Pitiot, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Few years ago, apricot fruits characterized by a deep red-purple supra-colour covering nearly the whole surface of the apricot fruit were found in the apricot germplasm, Being introduced in breeding programmes, preliminary selections were obtained and evaluated on their horticultural potential. Their interest for the consumers and their post-harvest main characteristics were investigated. While for the consumers the distinctiveness between these fruits and the conventional apricots was cleal, part of the interviewees was attracted by the innovation, while another part was not concerned. However, special attention has to be paid to the harvest because the intense red-colour masks the green ground colour of the skin. In addition, some of the accessions issued from this germplasm are characterized by a rather fast post-harvest evolution. Thus, in order to protect the consumers and to sell the best fruits as possible, it has been proposed to develop all the apricot series under the same trade mark 'Rubisco'® and under a technical agreement proposed to all the growers. So far, three cultivars have been named: 'Ravicille', 'Ravilong' and 'Ravival' with different dates of ripening.
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Determination of apricot fruit quality using Fourier-transform infrared spectroscopy
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Sylvie Bureau, David Ruiz, Maryse Reich, Barbara Gouble, Dominique D. Bertrand, Jean Marc Audergon, Catherine M.G.C. Renard
article
14. International Symposium, Jun 2008, Matera, Italy
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An apricot graft-compatibility evaluation device
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Marie Grotte, Eric Pietri, C. Pitiot, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
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An easy and reliable test to define apricot maturity
auteur
Barbara Gouble, Marie Grotte, Patrice Reling, Sylvie Bureau, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy. 1 p
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Carotenoid composition in apricot fruits of three cultivars from Italy: a preliminary investigation
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Annamaria Leccese, Sylvie Bureau, Maryse Reich, Catherine M.G.C. Renard, Jean Marc Audergon, Susanna Bartolini, Raffaella Viti
article
14. International Symposium, Jun 2008, Matera, Italy
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Study of the genetic basis of Prunus fruit quality on two apricot and two peach populations
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Jean Marc Audergon, David Ruiz, Alexandre Bachellez, Alain Blanc, Marie Noëlle Corre, Camille Croset, Anne Marie Ferréol, Patrick Lambert, Thierry Pascal, Jean-Luc Poessel, Veronique Signoret, Bénédicte Quilot, K. Boudheri, Christel Renaud, Elisabeth Dirlewanger, L. Dondini, Stefano Tartarini, S. Sansavini, Barbara Gouble, Marie Grotte, Marielle Boge, Patrice Reling, Maryse Reich, Sylvie Bureau, Catherine M.G.C. Renard, Catherine Deborde, Mickael M. Maucourt, Annick Moing, Sarah Monllor, Pere Arus
article
14. International Symposium, Jun 2008, Matera, Italy
resume
Within the ISAFRUIT European Integrated Project, a work package (6.1) is dedicated to the genetic bases of fruit quality and health properties. It is subdivided into six main tasks, two of them for the apricot and peach quality attrihutes: task I "Identification and mapping of candidate genes for fruit quality in saturated maps of peach and apricot" and task 3 'QTLs analysis of fruit quality". Six laboratories are involved in theses tasks on Prunus species and 4 Prunus progenies of 120 seedlings each are being used: two apricot progenies grown in Avignon (F) and Bologna (I), and two peach progenies grown in Avignon and Bordeaux (F). Fruit quality characterization is based on (i) physical measurements (fruit weight, colour, firmness), (ii) biochemical measurements (soluble solids content, titratable acidity) and (iii) fine metabolic profiling based on 1H RMN 1D targeting the major metabolites, associated with a phenolic compound characterization by HPLC. Saturated genetic linkage maps have been constructed for each progeny both in apricot and peach, and the phenotypic datasets will be joined with the genetic map for localising the associated QTLs. Identification and mapping of candidate genes for fruit quality traits will be the subsequent step. Particular attention will be paid to the co-linearity between species.
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New recent selections issued from INRA's apricot breeding programme
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Jean Marc Audergon, Alain Blanc, Frederic Gilles, J.M. Broquaire, Guy Clauzel, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, C. Pitiot
article
14. International Symposium, Jun 2008, Matera, Italy
resume
INRA, CENTREX and CEP INNOVATION are developing new apricot cultivars for French growers with particular attention paid to consumer's expectations. The main objectives of the related breeding programme are: prodnction regularity, fruit quality and attractiveness. Their evaluation is based on a multilocal and pluriannual set of trials located in the three major French areas of cultivation and organised as a 3-stage-valuation: (i) observation of the seedlings on their own roots (ii) evaluation of the best grafted selectious in the 3 main areas, (iii) assessment of the agronomic and commercial interest of the 'elite' accessions in small commercial orchards. At the end of the selection process two recent main types of cultivars have been introduced: - Ten new cultivars able to enlarge the harvesting season by 'conventional' type of fruit 'Soledane', 'Solimar'®, 'Candide', 'Bergerval'®, 'Bergarouge'®, 'Royal Roussillon', 'Vertige', 'Fantasme'®, 'Héléna du Roussillon'®, 'Frisson'); - Three new fruit-type apricots caracterized by a stark red blush covering 80% of the fruit shape, developed under the same trademark, 'Rubisco'® (Ravival, Ravilong and Ravicile).
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Comparative analysis of tunisian apricot cultivars based on morphological and molecular markers
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Lamia Krichen, Hedia Bourguiba, N. Trifi-Farah, M. Marrakchi, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
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An easy and reliable test to define apricot maturity
auteur
Barbara Gouble, Marie Grotte, Patrice Reling, Sylvie Bureau, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy. ⟨10.17660/ActaHortic.2010.862.85⟩
resume
Identification of maturity markers is essential to fix optimum harvest maturity and to ensure good apricot quality to the ultimate consumer. Skin degreening is the most common marker often used by fruit growers, but emergence of apricots with deep red coloured skin (purplish-blue) demands new methods to deline the optimum harvest date. Physical (colour of the flesh and the skin, perinuclear cavity volume) and physiological properties (ethylene production) of freestone apricots with various skin colours (white, orange, red) were studied during fruit growth on the tree. Development of the perinuclear cavity was proved correct as early marker of ethylene initiation in freestone apricot. Whatever the skin colour, it anticipated or was simultaneous with ethylene initiation that induces apricot ripening. An easy and reliable test for starting harvest is proposed, which consists in cutting the fruit along the suture line and twisting the two fruit halves in opposite directions. When no flesh adherence to the stone on both halves is observed, the fruit is able to ripen normally after picking:, so harvest can begin. However, best organoleptic quality will be obtained when fruit is harvested not too far from the physiological maturity.
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ISAFRUIT European project: QTLs for quality traits in apricot
auteur
David Ruiz, L. Dondini, M. Adami, C. Cervellati, P. de Franceschi, Sylvie Bureau, Barbara Gouble, Maryse Reich, Catherine M.G.C. Renard, Stefano Tartarini, Patrick Lambert, Daniele Bassi, S. Sansavini, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy
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An apricot graft-compatibility evaluation device
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Marie Grotte, Eric Pietri, C. Pitiot, Jean Marc Audergon
article
14. International Symposium, Jun 2008, Matera, Italy. ⟨10.17660/ActaHortic.2010.862.62⟩
resume
Apricot graft-compatibility is a major concern for most of the new released cultivars because they are often selected and rapidly evaluated on rather young trees, and then released without any graft-compatibility evaluation . Thus, fruit growers are directly facing breakages induced by graft-incompatibility between cultivars and root-stocks in orchard without any preventive indicator. In localized graft-incompatibility the focus is on the lack of continuity between the tissues of the root-stock and the cultivar. In order to estimate the level of fragility of the graft-union a mechanical tester bas been developed. The principle is based on the evaluation of tbe force to be applied to break the graft union. The data collected are based on the force, the pressure, the moment, the work and the diameter of the graft-combinations, all along the traction phase from thc beginning to the breakage point. The tester has been evaluated with 40 combinations of around 10 replications each in order to appreciate the internal and external variations. The preliminary results allow the separation of the graft combinations on the base of their elasticity, pressure and the surface of the junction between stock and scion.
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How different allelic combinations at resistance QTLs control the multiplication of Globodera pallida populations?
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Bernard Caromel, Didier Mugniery, Marie-Claire Kerlan, Sylvain Fournet, Véronique Lefebvre
article
2. Annual Meeting of the COST 872, May 2008, Postojna, Slovenia
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Durability of resistance to Globodera pallida from Solanum vernei, S. spegazzinii and S. sparsipilum
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Didier Fouville, Didier Mugniery, Olivier Plantard, Sylvain Fournet, Bernard Caromel, Marie-Claire Kerlan, Eric Grenier
article
2. Annual Meeting of the COST 872, May 2008, Postojna, Slovenia
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A new view on aphid resistance in melon: the role of Aphis gossypii variability
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Nathalie Boissot, Pascale Mistral, Virginie Chareyron, Catherine Dogimont
article
9. congrès international Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae, Institut National de la Recherche Agronomique (INRA). FRA., May 2008, Avignon, France
resume
Resistance to Aphis gossypii in Cucumis melo has been largely studied but A. gossypii variability has never been considered. Resistance to colonization by A. gossypii, clone NM1, and to non persistent virus transmission by this clone is conferred by a NBS-LRR gene called Vat, isolated in the PI 161375 accession. We investigated resistance to A. gossypii with four clones of A. gossypii that belong to two very distinct genotypes, NM1 and C9. The Vat gene conferred a high level of resistance to a NM1 clone and partial resistance to a C9 clone. Four additive QTL and two pairs of epistatic QTL were detected in a recombinant inbred line population derived from the cross ‘Védrantais’ x PI 161375. Half of them clearly have a specific effect on the acceptance by NM1 or C9 genotypes. We observed transgressive lines more resistant to NM1 or C9 in our RIL populations than PI 161375. Moreover, we studied resistance to A. gossypii and to virus transmission by A. gossypii in a set of 21 C. melo accessions. All the accessions resistant to virus transmission by C9 A. gossypii were resistant to virus transmission by NM1 genotype, and most of them were resistant to acceptance and colonization by the NM1 genotype. This is the phenotype of PI 161375, and we hypothesized that these phenotypes were controlled by the Vat gene. Other phenotypes were observed: resistance to NM1 A. gossypii (aphids and virus transmission) and susceptibility to C9 A. gossypii (aphids and virus transmission), independence between aphid resistance and resistance to virus transmission when using the same clone of A. gossypii. These phenotypes may be conferred by the locus Vat (different alleles) or by other locus of aphid resistance.
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Histological study of shoot organogenesis in melon (Cucumis melo L.) after genetic transformation
auteur
Veronique Chovelon, Veronique Restier, Catherine Dogimont, Jawad Aarrouf
article
9. Eucarpia Meeting on Gentics and Breeding on Cucurbitaceae, May 2008, Avignon, France
resume
Leave and cotyledon explants from two melon genotypes ‘Vedrantais’ and ‘Paul’ were cultured on different media and their regeneration was followed during 60 days. High frequency of plant regeneration was observed with ‘Vedrantais’ cotyledons cultured on MS medium added with BAP and 2-iP. Using this protocol, Agrobacterium mediated transformation was developed and a significant reduction of the plant regeneration rate was observed. Explants histological analysis revealed the presence of transformed meristematic areas but a rapid and premature cell vacuolization was observed. During plant regeneration, meristematic cells were affected by important cytological damages: cell dislocation accompanied by cell death and final collapsus. This disruption of meristematic areas may be related to an effect of A. tumefaciens transformation on melon cells and would explain the difficulties encountered to regenerate plants.
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Molecular diversity at the Vat/Pm-W resistance locus in melon
auteur
Catherine Dogimont, Veronique Chovelon, Sarah Tual, Nathalie Boissot, Vincent Rittener-Ruff, Nathalie Giovinazzo, Abdelhafid A. Bendahmane
article
9. Congrès international Eucarpia Meeting on Genentics and Breeding of Cucurbitaceae, Institut National de la Recherche Agronomique (INRA). FRA., May 2008, Avignon, France
resume
The resistance Vat gene of melon was isolated by map-based cloning. It encodes a protein with a coiled coil domain (CC), a nucleotide binding site (NBS) and leucine–rich repeats (LRR). Whereas most resistance genes confer resistance to a single pathogen, two alleles with different resistance specificities were identified at the locus. The Vat allele confers resistance to plant colonization by the aphid Aphis gossypii and to transmission of unrelated viruses by this vector specifically; the Pm-W allele confers resistance to the powdery mildew Podosphaera xanthii. Here we identified Vat-analogs in several melon genotypes using Long Range PCR, cloning and sequencing of full-length fragments. Several sources of variability were identified: (i) a different number of analogs were identified, suggesting a variable complexity of the gene cluster depending on the genotype, (ii) the number of repeats of a conserved motif of 65 amino acids within the LRR domain varied from two to five; this variation may be involved in the specific recognition of aphids or powdery mildew, (iii) finally, while the Vat allele seems to be shared by several aphid resistant accessions from various geographical origins, other alleles (genes) may be responsible for aphid resistance in some accessions; thus, a new putative allele was identified in the Indian accession 90625, which exhibits a resistance phenotype slightly distinct from that mediated by the Vat allele.
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https://hal.inrae.fr/hal-02758122/file/Dogimont_1997_1.pdf BibTex
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Inheritance of Podosphaera xanthii resistance in melo "90625
auteur
Michel Pitrat, Didier Besombes
article
9. Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae, May 2008, Avignon, France
resume
Several races of Podosphaera xanthii have been described on melon. The Indian melon line ‘90625’ (PI 313970) which had already been described as resistant in the USA is a potential source of resistance for strains recently characterized in France. We have studied the inheritance of resistance in a Recombinant Inbred Line population between ‘Védrantais’ (susceptible) and ‘90625’ using a leaf disk technique with 10 strains of P. xanthii. Potentially five genes could be present in ‘90625’. Two independent semi-dominant genes (A and B) are involved in resistance to strains 06Sm10 and S87-7 respectively. Resistance to the other strains seems to be under a digenic control, including A and B or not, with three other recessive genes. Some of these RILs could be used as additional differential lines for the characterization of the races.
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Pleiotropic effect of sex expression on fruit shape in melon
auteur
M.E. Abdelmohsin, Michel Pitrat
article
9. Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae, May 2008, Avignon, France
resume
In melon, monoecy is conferred by the dominant allele A. Many melon cultivars of different horticultural groups are andromonoecious, a/a. For facilitating commercial production of F1 hybrid seeds, a monoecious female parent would be quite useful. However, monoecy is almost always associated with a larger length-todiameter ratio of the fruit. We have tested the hypothesis that monoecy itself, allele A, is responsible for the change in fruit shape by using two sets of monoecious/andromonoecious near-isogenic lines: one derived by backcrossing A from a monoecious donor using the andromonoecious ‘Charentais T’ as the recurrent parent and one using the residual heterozygosity (H-NIL) after 6 generations of selfpollinating in a recombinant inbred line population. In both sets of near-isogenic lines, the length of the ovary and the length of the mature fruits were significantly larger in the monoecious lines than in the corresponding andromonoecious lines. The ovary diameter differed in the H-NIL set but not in the ‘Charentais’ set, while the diameter of the mature fruit was different in the ‘Charentais’ set but not in the H-NIL set. The fruit shape (ratio of length to diameter) did not change during fruit growth except for ‘Charentais mono’, which decreased. The results are consistent with the idea that the larger ovary and fruit lengths in monoecious lines are a pleiotropic effect of the absence of stamens in the female flowers (allele A). In order to obtain round monoecious lines, breeders will have to select the favourable QTLs involved in the genetic control of fruit shape.
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Genetic, genomic and ecophysiological dissection of fruit size and sugar content in tomato
auteur
Mathilde M. Causse, Marion Prudent, Nadia Bertin
article
16. EUCARPIA Meeting Section Vegetables Working Group Tomato, May 2008, Wageningen, Netherlands. 1p
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https://hal.inrae.fr/hal-02812667/file/49269_20120110110413337_1.pdf BibTex
titre
Emergence dates of Eurytoma amygdali enderlein adults in the south-east of France and control strategy.
auteur
Henri Duval, M. Millán
article
14. Grempa Meeting on Pistachios and Almonds, Mar 2008, Athens, Greece
resume
Eurytoma amygdali Enderlein was discovered in France in 1981 and is now present in the South-east area of France, but is absent in Pyrénées-Orientales and Corsica. In this study, the adult emergence dates in 2005, 2006 and 2007 have been observed in three different places. Some chemical control tests in insectarium have showed that the “Karate® Zeon” insecticide, with active ingredient the Pyrethroids ‘Lambda cyhalothrine’, has a high efficiency and retentiveness of more 23 days. These tests were confirmed in field orchards. With one single chemical spray applied at the beginning of the adult emergence, only 1 to 4 % of fruits were infested, when more than 60% of fruits were infested in the control almond orchard without insecticide. With these results, the Karate® Zeon has been legally authorized in France to be used against Eurytoma amygdali.
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https://hal.inrae.fr/hal-02758291/file/42466_20110222120536876_1.pdf BibTex
titre
Emergence dates of Eurytoma amygdali Enderlein adults in the southeast of France and control strategy
auteur
Henri Duval, M. Millán
article
14. Meeting of the Mediterranean Research Group for Almond and Pistachio, Mar 2008, Athènes, Greece
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Pyramiding mono and polygenic resistances is one strategy to provide lasting control of the resistance to the green peach aphid and powdery mildew in peach
auteur
Patrick Lambert, Marie-Hélène Sauge, Jean-Luc Poessel, Thierry Pascal
article
4. International Rosaceae Genomics Conference, Mar 2008, Pucon, Chile. pp.46
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https://hal.inrae.fr/hal-02818610/file/30315_20090513113751153_1.pdf BibTex
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Bin mapping candidates genes and SNP discovery in Prunus
auteur
E. Illa, Elisabeth Dirlewanger, Loick Le Dantec, Gaëlle Cardinet, Patrick Lambert, Jean Marc Audergon, C. Meneses, Pere Arus, W. Howad
article
4. International Rosaceae Genomics Conference, Mar 2008, Pucon, Chile. 1 p
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Genetic and proteomic approach of tomato fruit quality
auteur
Mireille Faurobert, Jamila Chaïb, Marion Barre, David Tricon, Stephane Munos, Mathilde M. Causse
article
1. International Symposium on Horticulture in Europe, Feb 2008, Vienne, Austria
resume
The organoleptic quality of tomato fruit is a complex characteristic involving a set of components such as fruit size, flaveur, aroma, texture. The research program on the genetic bases of fruit quality is focused on polymorphism evaluation and QTL characterization fur these traits. Research first mapped QTL controlling quality traits in several populations and introgressed quality trait QTL by marker-assisted selection into elite lines. This introgression allowed the production of Near-Isogenic Lines (NILs), which were characterized at several levels (quality components, metabolome, proteome and transcriptome). Subsequently a proteomic analysis of accessions and Near-Isogenic Lines (NILs) was developed to identify candidate proteins involved in the genetic variation of fruit quality. The analysis of total proteins allowed the quantification of about 1,8OO spots. Proteins showing either genetic or developmental variations were identified by MALDI-TOF and/or nano-I.C-MS/MS. The proteome was first characterized at 6 stages along fruit development of two varieties and then at two stages in 2O tomato accessions. Many spots showing differential expression were related to photosynthesis, protein turn-over, cell wall synthesis or degradation, carbohydrate metabolism, oxidative stress and senescence. Screening for proteins showing variation of quantity in NILs revealed some spots varying according to the allele at the QTL or according to the genetic background. The percentage of variation at protein level was close to the percentage of varying genes pointed out by transcriptome approach. Transcriptome and proteome analysis provided complementary results.
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Towards the use of modelling in genetic improvement: example of peach fruit quality
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Bénédicte Quilot-Turion, Michel M. Génard
article
1. International Symposium on Horticulture in Europe, Feb 2008, Vienne, Austria
resume
Improving fruit quality raises major difficulties. To overcome these difficulties, an interdisciplinary approach has been developed which consists in forwards and backwards between modelling, ecophysiological analysis and quantitative genetics. This approach has been applied to studies of fruit quality in peach. A population of 140 genotypes derived from a clone of wild peach (Prunus davidiana) by three generations of crosses with commercial cultivars of nectarine (Prunus persica) was studied and genotyped with 8O markers. The ecophysiological model predicts fruit and stone dry and fresh masses and total sugar concentration in relation to environmental conditions. Quantitative Trait Loci (QTL) were detected for all the model parameters and in many cases they were common to both years of experimentation. Co-locations between QTLs for quality traits and QTLs for parameters were observed. The QTL results were used to predict, for any genotype of the populations studied, the values of each parameter which were integrated into the ecophysiological model. This approach could provide a framework for understanding physiological and biological phenomena via the dissection of the quality traits into basic processes. The integration of genetic information into the ecophysiological model may be used for practical purposes, such as predicting the genotypic variations of a plant response to environmental conditions. It may also help to solve genotype and environment (GxE) interactions and to predict the behaviour of plants from the population with any combination of alleles under any climatic scenario.
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Profils et empreintes métaboliques par RMN du proton pour évaluer les modifications attendues et inattendues de la composition de tomates transgéniques
auteur
Yosra Ben Akal, Ethel Mendocilla Sato, Gwenaelle Spieldenner, Raphael Henry, Catherine Deborde, Stéphane Bernillon, Cécile Cabasson, Martine Dieuaide Noubhani, Aline Le Menn, Mickael M. Maucourt, Annick Moing, Christophe Rothan, Dominique D. Rolin
article
3.Journées Scientifiques, Feb 2008, Bordeaux, France
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Structure of molecular polymorphism in wild and cultivated tomato species (Solanum sp.) towards LD mapping for quality traits
auteur
Nicolas N. Ranc, Stephane Munos, Sophie Rolland, Marie-Christine Le Paslier, Esther Pelpoir, Sylvain S. Santoni, Jean-Paul Bouchet, Dominique D. Brunel, Mathilde M. Causse
article
International conference, Feb 2008, Vienne, Austria
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Mapping of QTLs for horticultural traits in three pepper populations and their association with resistance traits
auteur
Lorenzo Barchi, Véronique Lefebvre, Patrick Signoret, Anne-Marie Sage-Palloix, E. Portis, S. Lanteri, Alain Palloix
article
International conference, Feb 2008, Vienne, Austria
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Poster communications

titre
Expression or extinction of some eukaryotic translation initiation factor alleles in transgenic tomato and lettuce: effect on virus infection
auteur
Marianne Mazier, Raquel Sanjuan, Fabrice Flamain, Verane Sarnette, Sylvie S. German-Retana, André Moretti, Benoît Moury, Aline Le Menn, Patrick P. Rousselle, Brigitte B. Maisonneuve, Carole Caranta
article
International Conference, Nov 2008, Cadiz, Spain. , 2008, Genetic control of plant pathogenic viruses and their vectors: towards new resistance strategies. Book of abstracts
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Marker assisted selection of genes and QTLs for resistance combinations to Aphis gossypii in melon
auteur
Sophie Thomas, Pascale Mistral, Virginie Chareyron, Nathalie Boissot
article
9. congrès international Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae, May 2008, Avignon, France. Pitrat Michel, 2008, Cucurbitaceae 2008
resume
The Vat gene in melon confers resistance to both aphid colonization and aphid transmission of several viruses. QTLs that provide resistance to aphid were localized on the melon genome. We built genetic allelic combinations with/without the resistant allele at the Vat locus and QTLs. These combinations (homozygous for the resistance loci) had a homogeneous genetic background for 90 % of the genome. Plants with different allelic combinations were infested by two Aphis gossypii genotypes NM1 and C9, and observed for acceptance and biotic potential. The Vat gene reduced the acceptance by both aphid genotypes, but only reduced the biotic potential of NM1. QTLs had a complementary effect on acceptance. To predict the durability of the resistance we plan to investigate selection pressures that these combinations exert on aphid populations in field trials.
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QTL analysis of metabolites involved in fruit quality in Prunus species and co-location with candidate genes in the European Project ISAFRUIT - Preliminary results obtained on P. persica related species
auteur
Elisabeth Dirlewanger, Karima Boudehri, Gaëlle Cardinet, Christel Renaud, Camille Croset, Loick Le Dantec, Sarah Monllor, E. Illa, Patrick Lambert, Catherine Deborde, Mickael M. Maucourt, Bénédicte Quilot-Turion, Jean Marc Audergon, W. Howad, Annick Moing, Jean-Luc Poessel, Pere Arus
article
4. International Rosaceae Genomics Conference, Mar 2008, Pucon, Chile. 1 p., 2008
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Profils métaboliques de pêche: une étude des métabolites primaires et secondaires par RMN-1H et HPLC de deux descendances révèle de profondes variations entre génotypes et la présence de composés inattendus.
auteur
Jean-Luc Poessel, Camille Croset, Christel Renaud, Bénédicte Quilot, Catherine Deborde, Elisabeth Dirlewanger, Mickael M. Maucourt, Sarah Monllor, Veronique Signoret, Thierry Pascal, Pere Arus, Annick Moing
article
Réseau français de métabolomique et fluxomique : troisième journée scientifique, Feb 2008, Bordeaux, France. 2 p., 2008
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Diversité de virulences du Bremia lactucae dans un bassin de production
auteur
Brigitte B. Maisonneuve
article
7. Rencontres de Phytopathologie/Mycologie de la Société Française de Phytopathologie, Jan 2008, Aussois, France. poster 9, 2008
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https://hal.inrae.fr/hal-02751195/file/Maisonneuve-Poster-Bremia-Aussois2008_1.pdf BibTex

Reports

titre
Etude descriptive et comparative de collections publiques et privées de solanacées à graines
auteur
Guillaume Roch
article
[Stage] France. Université Claude Bernard Lyon 1 (UCBL), Villeurbanne, FRA. 2008, 82 p
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Structuration de la diversité génétique chez le melon Cucumis melo L
auteur
Laurana Serres-Giardi
article
[Stage] Institut National d'Etudes Supérieures Agronomiques de Montpellier (Montpellier SupAgro), Montpellier, FRA.; Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2008, 33 p
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Le melon Cucumis melo L. est une espèce d’importance horticole cultivée mondialement. Très diversifiée du point de vue morphologique, elle a connu une longue histoire de sélection et présente de nos jours 16 variétés botaniques aux types de fruits bien distincts. La structuration de la diversité génétique de l’espèce et l’histoire de sa domestication restent cependant mal connus. Connaître la structure et la diversité d’une espèce permet pourtant de mieux conserver et exploiter ses ressources. Une étude des patrons de diversité génétique et de la structuration d’une collection de 369 melons représentatifs de la diversité phénotypique connue est menée, en utilisant 24 marqueurs microsatellites répartis sur tout le génome. L’utilisation d’un algorithme de regroupement basé sur un modèle, implémenté dans le logiciel Structure, et de méthodes de regroupement sur des distances génétiques a permis de détecter 3 niveaux de structuration chez le melon : 2 groupes coïncidant avec la scission en sous-espèces, 5 groupes à forte signification géographique et 16 groupes significatifs à l’échelle des variétés botaniques et cultigroupes. L’assignation de melons sauvages à plusieurs de ces groupes et les niveaux de diversité observés accréditent l’hypothèse d’au moins trois domestications indépendantes, en Asie de l’Ouest, en Inde et en Afrique
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Rôle du gène MDHAR dans la teneur en vitamine C et le calibre du fruit chez la tomate
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Noé Gest
article
[Stage] Université d'Avignon et des Pays de Vaucluse (UAPV), Avignon, FRA. 2008, 33 p
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2007

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Conservation et valorisation des collections d'espèces légumières
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Michel Pitrat
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Les collections végétales vivantes. Place et rôles dans la conservation, Nov 2007, Nice, France
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Analyse du polymorphisme moléculaire dans le végétal
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Patrice P. This, Mathilde M. Causse, Véronique Jorge, Dominique D. Brunel
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Séminaire AIP INRA 2005-2007, Nov 2007, Paris, France
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Multiscale modelling of the emergence of virulent virus populations : towards new breeding strategies for building durable virus resistance
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Frederic Fabre, Claude Bruchou, Alain Palloix, Hervé Lecoq, Benoît Moury
article
10. International plant virus epidemiology symposium, Oct 2007, Patancheru, India
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Variability in odour emissions induced by the aphid Myzus persicae among peach cultivars and a wild related species expressing different types of resistance
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Hanane El Aouni, Benjamin Jackson, Bruno Buatois, Jean-Marie Bessière, Marie-Hélène Sauge, Jean-Luc Poessel, Jean Philippe Lacroze, Michael Staudt
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International Science Meeting of the ESF research networks VOCBAS and INTROP "Biogenic Volatile Organic Compounds Sources and fates in a changing world, Oct 2007, Montpellier, France. 1 p
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Mapping of key horticultural traits related to fruit quality, plant architecture and plant development in pepper (Capsicum annuum L.)
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Lorenzo Barchi, Véronique Lefebvre, Alain Palloix, S. Lanteri
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51. Annual congress Italian Society of Agricultural Genetics, Sep 2007, Riva del Garda, Italy
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Detection of QTLs controlling major fruit quality components in peach within the european project ISAfruit
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Elisabeth Dirlewanger, Gaëlle Cardinet, Karima Boudehri, Christel Renaud, Sarah Monllor, C. Crosset, Mickael M. Maucourt, Catherine Deborde, Jean-Luc Poessel, Annick Moing
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12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain
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ISAFRUIT: Map saturation of the advanced BC2 population peach x P. davidiana
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E. Illa, W. Howad, Patrick Lambert, Bénédicte Quilot, Jean Marc Audergon, Pere Arus
article
12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain. 1p
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ISAfruit ip project - genetic and molecular bases of peach and apricot fruit quality
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Jean Marc Audergon, Alexandre Bachellez, Alain Blanc, Marielle Boge, Karima Boudehri, Sylvie Bureau, Marie Noëlle Corre, Camille Croset, Catherine Deborde, Elisabeth Dirlewanger, L. Dondini, Anne Marie Ferréol, Barbara Gouble, Marie Grotte, Patrick Lambert, Mickael M. Maucourt, Annick Moing, Sarah Monllor, Thierry Pascal, Jean-Luc Poessel, Bénédicte Quilot, Maryse Reich, Patrice Reling, Christel Renaud, David Ruiz, Veronique Signoret, Pere Arus
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12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain
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ISAFRUIT. Study of the genetic basis of Prunus fruit quality in two peach and two apricot populations
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Jean Marc Audergon, David Ruiz, Alexandre Bachellez, Alain Blanc, Marie Noëlle Corre, Camille Croset, Anne Marie Ferréol, Patrick Lambert, Thierry Pascal, Jean-Luc Poessel, Veronique Signoret, Bénédicte Quilot, Karima Boudehri, Christel Renaud, Elisabeth Dirlewanger, L. Dondini, Barbara Gouble, Marie Grotte, Marielle Boge, Patrice Reling, Maryse Reich, Sylvie Bureau, Catherine Deborde, Mickael M. Maucourt, Annick Moing, Sarah Monllor, Pere Arus
article
12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain
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A European Integrated Project, untitled ‘ISAFRUIT’, has been engaged in January 2006. It deals with the improvement of fruit consumption by the consumers under both objectives: the optimization of the quality of the row products, and the optimization of the cultural practices. The project involves 40 Research Institutes and 20 Companies issued from 14 European countries and two associated countries. Within the whole project, a work package (6.1) is dedicated to the study of the genetics of fruit quality and health properties. It is subdivided in six main tasks, among them two concerning peach and apricot quality attributes have been already engaged: Task 1 – Identification and mapping of candidate genes for fruit quality in saturated maps of peach and apricot. Task 3 – QTLs analysis of fruit quality. Six research laboratories are involved in theses parts of the project based on the analysis of four Prunus progenies of 120 hybrid each, two apricot progenies cultivated in Avignon (Fr) and Bologna (It), and two peach progenies cultivated in Avignon and Bordeaux (Fr). Fruit quality characterization is based on (i) physical measurements (fruit weight, colour, firmness), (ii) biochemical measurements (refractometric index, titratable acidity) and (iii) on a fine metabolic profiling based on 1H RMN 1D, targeting the major metabolites, associated with a phenolic compounds characterization by HPLC. The related datasets will be joined with the genetic map under saturation for localising the associated QTLs. A particular attention will be paid to the co-linearity between species.
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Joint experiments in France and Catalonia of new flat peaches-nectarines and canning peaches from INRA
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Thierry Pascal, Iglesias I. Castellarnau, P. Blanc, C. Pitiot
article
12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain
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For several years, INRA has been developing a peach [Prunus persica (L.) Batsch] breeding program aimed partly at diversification. The selection objectives are more especially to create new varieties of peaches and flat nectarines better adapted to the needs of the French and European markets, as well as providing new canning peach varieties better suited to the constraints of the canning industry. Taking into account the interest of the Spanish market for these two fruit types, a trial was settled at IRTA (Lleida) to evaluate their agronomic potential of production in the pedo-climatic conditions of Catalonia, potentially favorable to these cultivars of medium chilling requirements. The results obtained in Spain and in France for several characteristics of the fruit (skin color, flesh firmness, size, juice traits: °Brix and acid total Meq/100 ml) and of the tree (type of pruning, flowering season, thinning, production level, ripening dates) are presented. They concern mainly two yellow flat nectarines, ‘Mesembrine’ cov and ‘Oriola’ cov, protected under the trademark ‘Platerine’®; three yellow flat peaches, ‘Oriane’ cov, ‘Ornella’ cov, and ‘Ordigan’ cov; and four original canning peach cultivars, ‘Fergold’ cov, ‘Fercluse’ cov, ‘Ferlot’ cov, and ‘Exp 7040’ cov. Their interest and performance are discussed in comparison to reference varieties. All these cultivars developed by INRA are edited in France by CEP INNOVATION.
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New recent selections issued from INRA's apricot breeding program
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Jean Marc Audergon, Alain Blanc, Frederic Gilles, Guy Clauzel, J.M. Broquaire, Barbara Gouble, Marie Grotte, Maryse Reich, Sylvie Bureau, C. Pitiot, S. Lafond
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12. Eucarpia Symposium, Sep 2007, Zaragoza, Spain
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INRA, CENTREX, and CEP INNOVATION are developing new apricot cultivars for French growers with a particular attention paid to consumer’s expectations. The main objectives of the breeding program are: production regularity, fruit quality, and attractiveness. The cultivar evaluation is based on a three-stage-evaluation: (i) observation of the hybrid on its own roots during three consecutive years of production, (ii) evaluation of the best hybrids after grafting in three representative areas of production during at least three consecutive years, (iii) assessment of the agronomic and commercial interest of the ‘elite’ accessions in pre-commercial orchards. At the end of the selection process, two main types of cultivars have been recently registered: five cultivars of conventional apricot type of fruit dedicated to the enlargement of the production period, three cultivars of new red-apricot type characterized by a stark red blush covering 80% of the fruit shape, developed under a same trade mark ‘Rubisco’ ®. All these apricot cultivars are diffused by CEP INNOVATION SARL.
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Detection of QTLs controlling major fruit quality components in peach within the European project ISAFRUIT
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Elisabeth Dirlewanger, G. Cardinet, Karima Boudehri, Christel Renaud, Sarah Monllor, E. Illa, W. Howad, Pere Arus, Camille Croset, Jean-Luc Poessel, Mickael M. Maucourt, Catherine Deborde, Annick Moing
article
12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain
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ISAFRUIT is a European integrated project aimed at increasing fruit consumption with the goal to improve health of the European citizens. One of the objectives of the work package GENFRUIT is to set the genetic basis of fruit quality by mapping the major genes and QTLs involved (Work package 6.1). This is performed on two peach (Prunus persica L. Batsch) and two apricot (Prunus armeniaca L.) progenies. Only results obtained on one of the two peach progenies, the F2 progeny of 207 hybrids issued from the ‘Ferjalou’ Jalousia® x ‘Fantasia’ cross (JxF), will be described in this paper. This progeny is segregating for six Mendelian traits, peach or nectarine (G), flat or round fruit (S), non-acid or acid fruit (D), clingstone or freestone (F), male sterility (Ps), no fruit at maturity (af), as well as for quantitative characters involved in fruit quality. This progeny was evaluated for various characters such as blooming and maturity date, fruit fresh, and dry weight, flesh firmness, juice soluble solid content; pH and acidity. The quantification of the flesh concentration in 15 to 20 major metabolites (glucose, fructose, sucrose, citrate, malate, major amino acids, and some major secondary metabolites) was performed in Bordeaux using proton NMR spectroscopy of polar extracts. Phenolics profiling, using HPLC-DAD, was performed in Avignon. Candidate genes involved in fruit sugar or acid contents and fruit development were bin mapped in the reference map ‘Texas’ x ‘Earlygold’ and in JxF. Preliminary results on QTL detection concerning fruit quality characters are presented. First colocalisations of QTLs with candidate genes were identified. In the future, the colocalisation of QTLs with candidate genes, and the colinearity of QTLs detected on the different populations analyzed in the ISAFRUIT project will be examined.
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Mapping the resistance to Plum Pox Virus (PPV) carried by the apricot 'stark early orange' using different environmental conditions and genetic backgrounds; comparison with P. davidiana
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Patrick Lambert, I. Karayiannis, Jean-Luc Poessel, M. Rubio, F. Dicenta, Jean Marc Audergon, Véronique Decroocq, Thierry Pascal, Marie Foulongne, V. Shurdi-Levraud, Veronique Restier, Alexandre Bachellez
article
12. Eucarpia Symposium, Sep 2007, Zaragoza, Spain
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Sharka disease is now recognised as the most economically detrimental virus disease in stone-fruit worldwide and numerous breeding programs that aim to select new cultivars resistant to PPV are under progress. For apricot, an important source of resistance has been identified in ‘Stark Early Orange’, a North-American cultivar. It has been used at INRA Avignon for the apricot PPV breeding program and for a rootstock-breeding program aimed at PPV resistance. For peach, P. davidiana is the only known source for resistance; it has been widely used for introducing PPV resistance into the closely related P. persica genome. Several progenies derived from these resistance sources were developed for these programs at the INRA Avignon as well as the associated genetic maps. Additionally, a number of resistance genes were cloned at the INRA Bordeaux and assigned to these maps. These progenies segregate for the PPV resistance and phenotypic evaluations were performed under contrasting environmental conditions including different PPV strains in order to explain the inheritance of the trait and map the loci involved in the resistance. This work was carried out by a group of researchers working in different fields of activities focused on PPV resistance and we propose in this paper an overview of the main findings.
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Evaluation of the current apricot variability in Tunisia. Comparison with previously described cultivars
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Lamia Krichen, N. Trifi-Farah, M. Marrakchi, Jean Marc Audergon
article
12. Eucarpia Symposium, Sep 2007, Zaragoza, Spain
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Apricot is widely cultivated in Tunisia showing a large variability among apricot landraces. Recent surveys performed in the main areas, lead to the identification of 112 accessions, 76 grafted ones issued from the northern, central, and south-eastern areas, and 36 seed-propagated ones, called ‘Bargougs’, encountered in the oasis areas. Their characterization has been assessed with morphological characters by using a subset of the UPOV descriptor, based on fruit and leaves quantitative traits. The apricot landraces characterization allowed the identification of a large variability w,hich is structured in two main groups related to fruit characters and enabled the distinction between ‘Bargougs’ accessions and varieties. Within the variety group, homogenous sub-groups have been highlighted and defined as ‘population cultivars’. Some original and interesting characters have been identified related to fruit colour, fruit shape and aroma; they will be useful for future breeding programs. Tremendous genetic erosion has been shown in the Tunisian apricot landraces by comparison with previous studies. This has to be taken in consideration by the creation of an accurate genetic resource collection.
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Rapid and non-destructive determination of soluble solids content and titratable acidity in apricot using near-infrared spectroscopy
auteur
David Ruiz, Jean Marc Audergon, Sylvie Bureau, Marie Grotte, Catherine M.G.C. Renard, Barbara Gouble, Maryse Reich
article
12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain
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Near-infrared (NIR) spectroscopy technique was used for a rapid and robust accurate determination of apricot fruit quality traits such as titratable acidity (TA) and soluble solids content (SSC). Experiments were carried out during two consecutive years (2005 and 2006). In 2005, four apricot cultivars (‘Badami’, ‘Bergeron’, ‘Goldrich’, and ‘Moniquí’) and four apricot selections (‘A 3759’, ‘A 3844’, ‘A 4034’, and ‘Iranian selection’) from different genetic origins and covering a large phenotypic variability in apricot fruit characteristics were collected at different maturity stages. In 2006, 183 hybrids coming from the cross ‘Goldrich’ x ‘Moniquí’ were analyzed. Both FT-NIR spectra on intact fruits and biochemical measurements (SSC and TA) were performed in representative samples. The FT-NIR spectra were measured using an integrating sphere (diffuse reflectance). Partial least-squares (PLS) regression was applied to develop NIR calibration models which were cross validated to evaluate the prediction ability. Statistical tools as determination coefficient (R2) and Root Mean Square Error of Prediction (RMSEP) were used to evaluate the performance of developed models. NIR models allowed a good prediction of soluble solids content (SSC) and titratable acidity (TA) (for example in 2005, models showed R2 values of 0.84 and 0.79, and RMSEP values of 0.98 and 3.62 for SSC and TA respectively). The NIR measurement technique allowed the determination of biochemical quality attributes (soluble solids content and acidity) in apricot fruit. Compared to traditional method, the NIR method is faster and non-destructive, which could be very useful for the apricot fruit industry
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Evolution of apricot fruit quality attributes in the new released cultivars
auteur
David Tricon, Hedia Bourguiba, David Ruiz, Alain Blanc, Jean Marc Audergon, Guy Clauzel, S. Semon, Sylvie Bureau, Barbara Gouble, Marie Grotte, Maryse Reich, Catherine M.G.C. Renard, Richard Brand
article
12. Eucarpia Symposium, Sep 2007, Zaragoza, Spain
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Among fruit species, apricot (Prunus armeniaca L.) is characterized by a large increase in the number of registered and/or granted cultivars over the last decades, and by an effective interest at the European level. At the same time, an evolution concerning fruit quality attributes was observed globally among registered cultivars. In order to characterize this tendency, 172 cultivars in collection were observed. Representative fruit samples have been collected and their quality attributes such as fruit weight, firmness, colour, soluble solids content, and titratable acidity were considered. The impacts of genetic and geographic origin factors were evaluated. A significant increase in fruit firmness and colour, as well as a decrease in fruit acidity, was observed. No evolution on sugar content was noticed.
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Study of an UBX gene sub-family that is preferentially expressed in pollen: functional genes or pseudogenes ?
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Jean-Luc Gallois, Jan Drouaud, Alain Lecureuil, Philippe Guerche
article
7. Colloque national de la SFBV, Société Française de Biologie Végétale (SFBV). FRA., Sep 2007, Versailles, France
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Study of an UBX gene sub-family that is preferentially expressed in pollen: functional genes or pseudogenes ?. 7. Colloque national de la SFBV
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Breeding for durability of disease resistance: analysis of the pepper-PVY interaction delivered new screening criteria for the breeder
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Alain Palloix, Valérie Ayme, Bérenger Janzac, Ghislaine Nemouchi, Pascale Mistral, Carole Caranta, Vincent Simon, Benoît Moury
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13. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2007, Warsaw, Poland
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Eggplant and Capsicum peppers : Historical texts and images
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M-Christin Daunay, Evelyne Jullian, Anna Whipkey, Jules Janick
article
13. Eucarpia Meeting on Genetics and Breeding of Capsiscum and Eggplant, Sep 2007, Warsaw, Poland
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Genetics and QTL analysis of fertility restoration in cytoplasmic male sterile pepper (Capsicum annuum L.)
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L.H. Wang, B.X. Zhang, Véronique Lefebvre, S.W. Huang, Anne-Marie Daubèze, Alain Palloix
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13. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2007, Warsaw, Poland
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Horticultural traits mapping and their association with resistance traits in pepper genome. Consequence for multi-trait breeding using marker assisted selection
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Lorenzo Barchi, Véronique Lefebvre, Patrick Signoret, Anne-Marie Sage-Palloix, Sergio Lanteri, Alain Palloix
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13. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2007, Warsaw, Poland
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Mechanism and spectrum of action of the resistance conferred by the Pvr4 gene in pepper to potyviruses and selection of virulent variants
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Bérenger Janzac, Benoît Moury, Marie-Françoise Fabre, Alain Palloix
article
13. Eucarpia meeting on Genetics and Breeeding of Capsicum and Eggplant, Sep 2007, Warsaw, Poland
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Analysis of diversity in pepper genetic resources: distribution of horticultural and resistance traits in the INRA pepper germplasm
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Anne-Marie Sage-Palloix, Francoise Jourdan, Therese Phaly, Ghislaine Nemouchi, Véronique Lefebvre, Alain Palloix
article
13. Eucarpia meeting on genetics and breeding of Capsicum and eggplant, Sep 2007, Warsaw, Poland
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Host factors required in plant-potyvirus interactions: role of initiation translation factor eIF4E and eIF4G
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Sylvie S. German-Retana, Valerie Nicaise, Bénédicte Doublet, Jocelyne J. Walter, Genevieve Roudet, Thierry Michon, Thierry Candresse, Carole Caranta, Olivier Le Gall
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13. International congress on molecular plant-microbe interactions, Jul 2007, Sorrento, Italy
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Towards the elucidation of genetic and molecular basis of resistance against cucumber mosaic virus in Arabidopsis thaliana
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Julien Petit-Pierre, Vincent Rittener, André Moretti, Mireille Jacquemond, Catherine Dogimont, Carole Caranta
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13. International congress on molecular plant-microbe interactions, Jul 2007, Sorrento, Italy
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Contrasted evolution of eIF4E genes: conservation in Arabidopsis thaliana versus selection in pepper (driven by RNA viruses?)
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Carine Charron, Pascale Sanchez, André Moretti, Alain Palloix, Stéphane de Mita, Jacques David, Carole Caranta
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13. International congress on molecular plant-microbe interactions, Jul 2007, Sorrento, Italy
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Evolution de l'abricot au plan international. Etat de la culture et prospective. Diaporama
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Jean Marc Audergon
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Journées Suisses de l'Abricot, Jul 2007, Conthey-Chateauneuf/Sion, Suisse. 32 p
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La diversité de l'abricot : forces et faiblesses de l'espèce abricotier. Diaporama
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Jean Marc Audergon
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Journées Suisses de l'abricot, Jul 2007, Conthey-Chateauneuf/Sion, Suisse. 26p
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Amino acid diversity and functional analysis of eIF4E translation initiation factors in both model and cultivated species: a comparative study
auteur
Carole Caranta, Carine Charron, Maryse Nicolaï, Pascale Sanchez, André Moretti, Christophe Robaglia, Alain Palloix
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6. PTRoPGE, May 2007, Carry - le - Rouet, France
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Two resistance QTLs display induced and constitutive, as well as additive and epistatic effects, on transcription of defense-related genes
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Michel Claverie, Véronique de Conto, Katell Jolivet, Marie-Claire Kerlan, Eric Grenier, Didier Mugniery, Véronique Lefebvre, Bernard Caromel
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1. Annual Meeting COST 872, May 2007, La Colle-sur Loup, France
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Use of genomic information in a program of sustainable resistance to Globodera pallida
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Marie-Claire Kerlan, Laura Chauvin, Eric Grenier, Didier Mugniery, Bernard Caromel
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1. Annual Meeting COST 872, May 2007, La Colle-sur Loup, France
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Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome
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Caroline Djian-Caporalino, Ariane Fazari, Marie-Jeanne Arguel, Tatiana Vernié, Céline van de Casteele, Ingrid Faure, Geraldine Brunoud, Lucette Pijarowski, Alain Palloix, Véronique Lefebvre, Pierre Abad
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1. Annual Meeting COST 872, May 2007, La Colle-sur Loup, France
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Perspectives génétiques. Diaporama
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Jean Marc Audergon
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La diversité de l'abricot dynamique technique et adaptation au marché CTIFL Balandran, Apr 2007, Balandran Bellegarde, France. 20p
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Screening of genetic resources for resistance to Xanthomonas axonopodis pv. vitians
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Brigitte B. Maisonneuve, C. Chassagne, Elodie Gautheron, Catherine Glaux, Cindy E. Morris
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Eucarpia leafy vegetables 2007, Apr 2007, Warwick, United Kingdom
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Application of molecular markers in breeding for resistance to the Plum Pox Virus in Prunus spp.
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Grégoire Marandel, Ophélie Sicard, Donna Lalli, Patrick Lambert, Thierry Pascal, Albert Abbott, Jaroslav Salava, Maria Luisa Badenes, Véronique Decroocq
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7. Journée scientifique, Apr 2007, Arcachon, France
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Implication des facteurs d'initiation de la traduction elF4E et elF4G dans le cycle des potyvirus
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Sylvie S. German-Retana, Valerie Nicaise, Jocelyne J. Walter, Bénédicte Doublet, Genevieve Roudet, Thierry Michon, Cécile Lecampion, Marie-Christine M.-C. Houvenaghel, Christophe Robaglia, Carole Caranta, Olivier Le Gall
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11. Rencontres de virologie végétale, Jan 2007, Aussois, France
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Modélisation de l'évolution des populations virales pour la construction de variétés résistantes en cultures annuelles
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Frederic Fabre, Hervé Lecoq, Alain Palloix, Vincent Ginot, Benoît Moury
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11. Rencontres, Jan 2007, Aussois, France
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Organisation génomique des QTL de résistance au Plum Pox Virus chez Prunus davidiana, cartographie comparée avec l'abricotier et développement de premiers outils pour la sélection assistée par marqueurs
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Grégoire Marandel, Ophélie Sicard, Patrick Lambert, Thierry Pascal, M.L. Badenes, J. Salava, Véronique Decroocq
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11. Rencontres de virologie végétale, Jan 2007, Aussois, France
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Le TILLING : Un outil pour générer de nouveaux allèles de résistance au virus
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Maryse Nicolaï, Aurélie Salgues, André Moretti, Florence Piron, Elodie Piednoir, Abdelhafid A. Bendahmane, Carole Caranta
article
11. Rencontres de Virologie végétale, Jan 2007, Aussois, France
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titre
Évolution contrastée des gènes eIF4E : conservation chez A. thaliana versus sélection chez le piment (exercée par les virus à ARN ?)
auteur
Carine Charron, Pascale Sanchez, André Moretti, Alain Palloix, Stéphane de Mita, Jacques David, Carole Caranta
article
11. Rencontres de Virologie Végétale, Jan 2007, Aussois, France
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titre
La variabilité des gènes d'avirulence viraux conditionne t-elle la durabilité des résistances chez la plante
auteur
Bérenger Janzac, Frederic Fabre, Alain Palloix, Benoît Moury
article
11. Rencontres, Jan 2007, Aussois, France
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titre
Vers l'élucidation des bases génétiques et moléculaires de la résistance au CMV chez Arabidopsis thaliana
auteur
Julien Petit-Pierre, Vincent Rittener, André Moretti, Mireille Jacquemond, Catherine Dogimont, Carole Caranta
article
11. Rencontres de Virologie Végétale, Jan 2007, Aussois, France
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titre
Lutte génétique contre les bioagresseurs des cultures : la durabilité, un nouveau critère de sélection
auteur
Alain Palloix, Nathalie Boissot, Valérie Ayme, Bérenger Janzac, Hervé Lecoq, Cecile Desbiez, Frederic Fabre, Benoît Moury
article
4. Rencontres du Végétal, Jan 2007, Angers, France
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https://hal.inrae.fr/hal-02756913/file/36684_20100520113207134_1.pdf BibTex
titre
Application of molecular markers in breeding for resistance to the Plum Pox Virus in Prunus spp.
auteur
Grégoire Marandel, Ophélie Sicard, Donna Lalli, Patrick Lambert, Thierry Pascal, Albert Abbott, Boris Krška, Jaroslav Salava, Maria-Luisa Badenes, Véronique Decroocq
article
15. Plant & Animal Genome, Jan 2007, San Diego, United States
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titre
Application of molecular markers in breeding for resistance to the Plum pox virus in Prunus spp
auteur
Grégoire Marandel, Ophélie Sicard, Donna A. Lalli, Patrick Lambert, Thierry Pascal, Albert G. Abbott, Boris Krška, Jaroslav Salava, Maria Luisa Badenes, Véronique Decroocq
article
Plant and animal genome XV. The international conference on the status of plant and animal genome research, Jan 2007, San Diego, United States
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Master thesis

titre
Ancrage du génome du piment aux génomes de la tomate et de la pomme de terre et au génome d'Arabidopsis thaliana
auteur
Patrick Signoret
article
Sciences du Vivant [q-bio]. 2007
resume
Tous les organismes vivants actuels sont supposés avoir évolué à partir d’ancêtres communs et sont comparables sur la base de régions chromosomiques communes. La génomique comparative consiste à utiliser des espèces modèles (Arabidopsis thaliana, tomate…) pour décrire le génome plus complexe d’espèces phylogénétiquement proches. Des cartes génétiques du piment et de la pomme de terre ont été réalisées en partie avec des sondes moléculaires venant de la tomate. Dans ce contexte, trois cartes génétiques intraspécifiques du piment ont été développées au laboratoire dans le but de cartographier sur le génome et de caractériser des locus d’intérêt agronomique, notamment le locus Phy-P5 impliqué dans la résistance quantitative (QTL) au pathogène mycélien Phytophthora capsici. Dans le cadre de notre étude, un total de 191 marqueurs moléculaires a été localisé sur la carte F5YC du piment plus particulièrement dans la région de Phy-P5 dont la position a été précisée. Les trois cartes génétiques du piment ont été alignées entre elles et ancrées aux cartes génétiques de la tomate, de la pomme de terre et d’Arabidopsis thaliana avec des marqueurs communs. Les résultats obtenus confirment que nous pouvons bénéficier d’informations de génomique sur les espèces modèles notamment pour caractériser des locus d’intérêt. L’utilisation de nouvelles méthodes de génotypage à haut débit permettra de développer de nouveaux marqueurs ancres sur l’ensemble du génome, notamment dans le but de réaliser le clonage positionnel de Phy-P5, et de tester l’hypothèse d’une origine ancestrale de ce locus.
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Master thesis
Accès au texte intégral et bibtex
https://hal.inrae.fr/hal-02824655/file/41814_20110331084257973_1.pdf BibTex

Poster communications

titre
Analyse de la diversité de la tolérance et de l'accumulation de cadmium chez la laitue
auteur
Walid Zorrig, Brigitte B. Maisonneuve, Jean-Claude Davidian, Chedly Abdelly, Pierre Berthomieu
article
7. Colloque national de la SFBV, Sep 2007, Versailles, France. 2007, 7ème colloque national de SFBV
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https://hal.inrae.fr/hal-02755999/file/33884_20100611012418516_1.pdf BibTex
titre
Molecular determinants of recognition specificity at the aphid and powdery mildew Vat/Pm-W resistance locus in melon
auteur
Catherine Dogimont, Veronique Chovelon, Sarah Tual, Nathalie Boissot, Vincent Rittener, Nathalie Giovinazzo, Abdelhafid A. Bendahmane
article
13. International congress on molecular plant-microbe interactions, Jun 2007, Sorrento, Italy. 2007, 13. International congress on molecular plant-microbe interactions. Book of abstracts
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titre
Study of the genetic basis of Prunus fruit quality in 2 peach and 2 apricot populations
auteur
Patrick Lambert, Bénédicte Quilot, Jean Marc Audergon, Alexandre Bachellez, Alain Blanc, Marielle Boge, Karima Boudehri, Sylvie Bureau, Marie Noëlle Corre, Camille Croset, Catherine Deborde, Elisabeth Dirlewanger, L. Dondini, Anne Marie Ferréol, Barbara Gouble, Marie Grotte, W. Howad, Mickael M. Maucourt, Annick Moing, Sarah Monllor, Thierry Pascal, Jean-Luc Poessel, Maryse Reich, Patrice Reling, Christel Renaud, David Ruiz, S. Sansavini, Veronique Signoret, Stefano Tartarini, Pere Arus
article
2. ISAfruit General Assembly, Jun 2007, Bologne, Italy. 1 p., 2007
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titre
New interpretation of the resistance to Bremia lactucae of some differential lettuce varieties
auteur
Brigitte B. Maisonneuve
article
Eucarpia Leafy vegetables 2007 Conference, Apr 2007, Warwick, United Kingdom. 2007, Eucarpia Leafy vegetables 2007 Conference Abstracts
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titre
A new tool for gene tagging in lettuce (Lactuca sativa): the Tnt1 retrotransposon from tobacco
auteur
Marianne Mazier, Emmanuel Botton, Fabrice Flamain, Jean-Paul Bouchet, Béatrice Courtial, M-Christine M.-C. Chupeau, Yves Y. Chupeau, Brigitte B. Maisonneuve, Helene Lucas
article
Eucarpia Leafy vegetables 2007 Conference, Apr 2007, Warwick, United Kingdom. 2007, Eucarpia Leafy vegetables 2007 Conference Abstracts
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Reports

titre
Outil de gestion de données de séquences nucléotidiques et d'alignements
auteur
Philippe Nunez
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2007, 79 p
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titre
Amélioration des méthodes de régénération et de transformation génétique du melon (Cucumis melo L.) dans le cadre de la validation fonctionnelle du gène A
auteur
El-Batoul Djouani
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2007, 29 p
resume
L’objectif de mon stage est la mise au point d’un protocole de régénération par organogenèse et de transformation génétique avec Agrobacterium tumefaciens du melon (Cucumis melo L.) dans le cadre de la validation fonctionnelle du gène A. Deux génotypes ont été testés (Védrantais et Paul), ainsi que deux types d’explants (cotylédons pré-germés et feuilles), suivant deux protocoles (Mendi et Pech). Les résultats des expériences de régénération simple ont montré que le génotype Védrantais régénère mieux que Paul. L’étude de l’interaction entre ces trois facteurs (génotype, type d’explant et milieu de culture) a permis de définir les paramètres les plus favorables : la culture de cotylédons prégermés du génotype Védrantais sur le milieu de régénération de Pech (0,225 mg/L de BAP et 0,203 mg/L de 2.IP) permet le développement de bourgeons sur 70,66% des explants. Dans le but d’améliorer la transformation génétique, nous avons testé trois traitements au moment de la coculture : agitation des explants en présence de la bactérie (V0), utilisation du vide (V+) ou vortex des explants avec le carborundum (C+). L’estimation du niveau de transformation a été faite par le nombre d’explants transformés prélevés 2 et 20 jours après coculture et par leur niveau d’expression du gène GUS suivant une échelle de notation (de 0 à 5). Les résultats obtenus ont montré que le vide et le carborundum améliorent la transformation, mais diminuent la capacité de régénération des explants. Une comparaison entre la régénération des explants avec ou sans transformation a permis de montrer que le taux de régénération diminue fortement après transformation. Une étude cytologique a démontré que les cellules qui régénèrent sont également celles qui se transforment (cellules sous épidermiques) mais qu’environ 30 jours après coculture, ces cellules perdent leur organisation et dégénèrent progressivement ; ce qui peut expliquer le très faible taux de régénération de plantules transformées obtenu sur cette espèce.
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https://hal.inrae.fr/hal-02823248/file/22525_20080703013809390_1.pdf BibTex
titre
Etude de la résistance du melon à un virus émergent, le CVYV
auteur
Sylvie Chaumard
article
[Stage] Université d'Avignon et des Pays de Vaucluse (UAPV), Avignon, FRA. 2007, 34 p
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titre
Epidémiologie et diversité génétique de l'agent pathogène Bremia lactucae Regel en région PACA
auteur
Veronique Cruanas
article
[Stage] Université d'Avignon et des Pays de Vaucluse (UAPV), Avignon, FRA. 2007, 44 p
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titre
Les fruits et légumes dans l'alimentation : enjeux et déterminants de la consommation [Synthèse du rapport d'expertise]
auteur
Pierre P. Combris, Marie Josèphe Amiot, France Caillavet, Mathilde M. Causse, Jean Dallongeville, Martine Padilla, Catherine M.G.C. Renard, Louis-Georges Soler, . Unité Expertise Collective, Prospective Et Etudes
article
[0] INRA. 2007, 82 p
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https://hal.inrae.fr/hal-02818289/file/inra-fruits-legumes-synthese1_1.pdf BibTex
titre
Les fruits et légumes dans l'alimentation : enjeux et déterminants de la consommation [Rapport d'expertise]
auteur
Pierre P. Combris, Marie Josèphe Amiot, France Caillavet, Mathilde M. Causse, Jean Dallongeville, Martine Padilla, Catherine M.G.C. Renard, Louis-Georges Soler, . Unité Expertise Collective, Prospective Et Etudes
article
[0] INRA. 2007, 374 p
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titre
Caractérisation de la diversité moléculaire au locus Vat/Pm-W chez le melon
auteur
Xavier Grand
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2007, 25 p
resume
Le puceron Aphis gossypii (Glover) et Le champignon Podosphaera xanthii, agent responsable de l’oïdium, sont des ravageurs des cultures de melon. Il existe des accessions de melon montrant des phénotypes de résistance vis-à-vis de ces bio-agresseurs. Le locus Vat (Virus Aphid Transmission) est un locus de résistance qui confère la résistance au puceron A. gossypii. Le gène Vat, à ce locus, code pour une protéine de type coiled-coil nucleotide-binding leucine-rich-repeat. Deux allèles au locus Vat ont été identifiés jusqu’à présent : l’allèle Vat qui confère le phénotype de résistance au puceron et l’allèle Pm-W qui confère la résistance aux races 1,2 et 3 de P. xanthii. La différence majeure entre ces deux allèles est le nombre de répétitions d’un motif répété de 195 nucléotides au niveau de l’exon 2 du gène. Le nombre de ces répétitions est-il responsable de la spécificité de reconnaissance des facteurs d’avirulence d’A. gossypii et de l’oïdium ? Nous avons montré que la présence d’allèles et d’homologues du gène Vat présentant quatre motifs répétés est généralement associée au phénotype de résistance au puceron, et la présence d’allèles et d’homologues à cinq motifs répétés est associée au phénotype de résistance à l’oïdium. Les séquences obtenues chez l’accession 90625 montrent que la résistance au puceron A. gossypii n’est pas exclusivement conférée par l’allèle Vat.
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titre
Résistance à la bactériose foliaire de la laitue causée par Xanthomonas campestris pv. vitians : étude de géniteurs potentiels du genre Lactuca et analyse du déterminisme génétique
auteur
Perrine Moris
article
[Stage] Institut National d'Horticulture (INH), Angers, FRA. 2007, 48 p
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2006

Conference papers

titre
Profils métaboliques de la pêche et de l’abricot pour l’étude des bases génétiques de la qualité des fruits de Prunus dans le projet européen Isafruit
auteur
Jean-Luc Poessel, Annick Moing, Jean Marc Audergon, Alexandre Bachellez, Alain Blanc, Marielle Boge, K. Boudheri, Sylvie Bureau, Marie Noëlle Corre, Camille Croset, Catherine Deborde, Elisabeth Dirlewanger, L. Dondini, Anne Marie Ferréol, Barbara Gouble, Marie Grotte, Patrick Lambert, Mickael M. Maucourt, Sarah Monllor, Thierry Pascal, Bénédicte Quilot, Maryse Reich, Patrice Reling, Christel Renaud, David Ruiz, Veronique Signoret, Pere Arus
article
2. Journées Scientifiques du Réseau Français de Métabolomique et Fluxomique, Dec 2006, Saint Sauves d'Auvergne, France
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titre
Les Bactérioses foliaires chez la laitue : recherche de résistances
auteur
Brigitte Maisonneuve
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2006, Avignon, France
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titre
Un abricot de qualité ? Diaporama
auteur
Jean Marc Audergon
article
Miffel, Oct 2006, Avignon, France. 18 p
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titre
Caractérisation de la diversité génétique chez la tomate
auteur
Sheh May Tam, Mireille Faurobert, Tomasz Pawlowski, Cecile Garchery, Helene Burck, Corinne C. Mhiri, Mathilde M. Causse, Marie Angele M. A. Grandbastien
article
6. Colloque National du BRG, Oct 2006, La Rochelle, France
resume
Characterisation of tomato genetic diversity. In the period of agricultural intensification, modern plant breeding techniques have often resulted in drastic reduction of plant genetic diversity. Conservation of biodiversity is recognised as an important issue as many plant breeding programs routinely rely on germplasms as sources to develop agronomic traits of interest. Constant evaluation of plant genetic resources happens via the development of functional tools which accurately reflect existing diversity. Molecular markers are widely used to assay genetic diversity due to relative ease of utility, high polymorphism levels and furthermore, may be combined with other data, e.g. physiological approaches to facilitate direct targeting of interesting traits. In the framework of the French Solanaceae Biological Resources Center (CRB) to maintain diverse genetic resources, the Research Unit for Genetic Improvement of Fruit and Vegetables at INRA-Montfavet (UGAFL) holds a large collection of tomato (Solanum lycopersicum) and related wild species (Solanum subsection Lycopersicon). High phenotypic variation is displayed by the tomato fruit, especially in fruit size owing to domestication. UGAFL has accrued many accessions differing for fruit type, size and composition, well representing the natural variation observed for this genus. This collection thus provides the material basis for a number of breeding programs involved in improving pathogen resistance, nutritional and organoleptic qualities of tomato. Our project, in collaboration between UGAFL and the Laboratory of Cell Biology at INRA-Versailles, intended to characterise the genetic diversity of the collection comprising of 85 accessions, including 53 tomato lines/cultivars and 32 accessions of related wild species using different tools. We developed novel markers based on SSAP (Sequence-Specific Amplification Polymorphisms) analysis of insertion polymorphisms from three distinct families of copia-type retrotransposons (Tnt1, ToRTL1 and T135). Results show that combined SSAP insertion profiles of Tnt1, ToRTL1 and T135 are congruent and useful for inferring host-species phylogeny. Groups of species accessions were recovered,while the tree topology is in agreement with other previous phylogenetic analyses, distinguishing groups of red and green fruited species, self compatible and self incompatible species. This also demonstrates a strong association between hostspecies phylogeny and the distribution of retrotransposon insertions. Diversity within the tomato collection is much lower compared to wild species. Nonetheless, SSAP insertion profiles reflect the genetic background of the lines and several clear groupings according to fruit types were recovered, such as clusters of round or fleshy types with large fruits, as well as clusters of industrial-long self-pruning types. Most cherry and small-fruited types occupy less derived positions than larger-fruited tomatoes, agreeing with the hypothesis of small fruit size being an ancestral character. Altogether, our results show that retrotransposon insertion polymorphisms proved highly useful for diversity analyses by successfully genotyping all accessions and reflecting underlying genetic divergences of host genomes. Subsequent integration of SSAP markers with other physiological markers, such as proteomic markers, or with neutral markers, will be performed.
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https://hal.inrae.fr/hal-02758106/file/21217_20080408030427546_1.pdf BibTex
titre
Development of molecular bridge markers between Solanaceae species for comparative mapping of resistance loci to Phytophthora spp.
auteur
Sarah Danan, Patrick Signoret, Bernard Caromel, Véronique Lefebvre
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Etude transcriptomique de la résistance quantitative de la pomme de terre au nématode à kyste Globoderra pallida : validation de TDFs et recherche de gènes candidats par Q-PCR
auteur
Michel Claverie, Véronique de Conto Haby, Katell Jolivet, Eric Grenier, Marie-Claire Kerlan, Didier Mugniery, Véronique Lefebvre, Bernard Caromel
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Approches génomiques et moléculaires de la résistance aux bioagresseurs
auteur
Carole Caranta
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Cartographie haute résolution de QTL de résistance aux Phytophtora colinéaires entre le piment et la pomme de terre
auteur
Stephanie Mallard, Sarah Danan, Julien Bonnet, David Tricon, Patrick Signoret, Anne-Marie Sage-Palloix, Therese Phaly, Anne Blattes, Bernard Caromel, Nadia Lama, Jawad Aarrouf, Jean-Eric Chauvin, Alain Palloix, Véronique Lefebvre
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Déterminants de spécificité des interactions plantes-bioagresseurs: caractérisation moléculaire du locus de résistance Vat chez le melon
auteur
Catherine Dogimont, Veronique Chovelon, Sarah Tual, Vincent Rittener, Nathalie Giovinazzo, Jérôme Pauquet, Emilie Burget, Lynda Hagen, Nathalie Boissot, Michel Pitrat, Abdelhafid A. Bendahmane
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Effects of quality QTL introgression on tomato fruit proteome
auteur
Mireille Faurobert, Marion Barre, Jamila Chaïb, Mathilde M. Causse
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Towards the positional cloning of the QTL Phy-P5 affecting Phytophthora blight resistance in pepper
auteur
Julien Bonnet, David Tricon, Anne-Marie Sage-Palloix, Bernard Caromel, Abdelhafid A. Bendahmane, Alain Palloix, Véronique Lefebvre
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
L'association de 2 QTL est nécessaire pour conférer une résistance de haut niveau et à large spectre au nématode à kyste G. pallida chez la pomme de terre
auteur
Bernard Caromel, Didier Mugniery, Marie-Claire Kerlan, Michel Claverie, Laura Chauvin, Nadia Lama, Jean-Paul Dantec, Claudia Rouaux, Josiane Quéré, Daniel Ellisèche, Véronique Lefebvre
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Vers une sélection assistée par marqueurs chez la pomme de terre pour la résistance au nématode à kyste Globodera pallida
auteur
Marie-Claire Kerlan, Bernard Caromel, Didier Mugniery, Laura Chauvin, Jean-Paul Dantec, Sonia Madec, Josiane Quéré, Véronique Lefebvre, Daniel Ellisèche
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Caractérisation du polymorphisme des gènes eIF4E impliqués dans la résistance aux potyvirus et impact sur l'interaction avec ces virus
auteur
Carine Charron, Maryse Nicolaï, Jean-Luc J.-L. Gallois, Sandrine Ruffel, Benoît Moury, Carole Caranta
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Approche transcriptomique pour l'identification de gènes impliqués dans les interactions Arabidopsis thaliana /potyvirus
auteur
Valerie Schurdi-Levraud, - Feng Xiaoyan, Yannick Sourrigues, Maud M. Rivard, Johann J. Petit, Ludivine Taconnat, Christophe Rothan, Jean-Pierre J.-P. Renou, Olivier Le Gall, Carole Caranta, Frederic Revers
article
Rencontres Scientifiques du DGAP, Sep 2006, Batz sur mer, France
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titre
Watermelon resistance to powdery mildew race 1 and race 2
auteur
Angela Davis, Antonia Tetteh, Todd Wehner, Ammon Levi, Michel Pitrat
article
Cucurbitaceae 2006, Sep 2006, Asheville, United States
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titre
First images of Cucurbita in Europe
auteur
Harry Paris, Jules Janick, M-Christin Daunay
article
Cucurbitaceae 2006, Sep 2006, Asheville, United States
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titre
Cartographie génétique et physique fine des gènes A et G contrôlant le déterminisme sexuel du melon
auteur
Mohamed Fergany, Christelle Troadec, Mazen Rajab, Adnane Boualem, Marion M. Dalmais, Michel M. Caboche, Abdelhafid A. Bendahmane, Didier Besombes, Nathalie Giovinazzo, Catherine Dogimont, Michel Pitrat
article
Cucurbitaceae 2006, Sep 2006, Asheville, United States
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titre
Development of an image database of Cucurbitaceae
auteur
Jules Janick, Anna Whipkey, Harry Paris, M-Christin Daunay, Evelyne Jullian
article
Cucurbitaceae 2006, Sep 2006, Asheville, United States
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titre
A high resolution intra-specific linkage map of pepper (Capsicum annuum L.) based on RILs and identification of the most informative individuals
auteur
Lorenzo Barchi, Julien Bonnet, E. Portis, S. Lanteri, I. Nagy, Véronique Lefebvre, Alain Palloix
article
50. Annual Congress, Sep 2006, Ischia, Italy
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titre
Genetic and functional characterization of the peach - Myzus persicae interaction : towards breeding for durable resistance within integrated orchard management
auteur
Marie-Hélène Sauge, Thierry Pascal, Jocelyne Kervella, Jean Philippe Lacroze, Marie Noëlle Corre, Frédéric Pfeiffer, Isabelle Grechi, Francoise F. Lescourret, Annick Moing, Christel Renaud, Monique Gaudillère, Jean-Luc Poessel
article
Organisation Internationale de Lutte Biologique (OILB)- Section Régionale Ouest paléarctique - Groupe de travail protection intégré en vergers, Sep 2006, Ballandran, France. 1 p
resume
Integrated production of fruit and vegetable species is one major field of research at Avignon INRA centre. Breeding for durable resistance to the green peach aphid, Myzus persicae (Sulzer) and other enemies of peach crop (Prunus persica L. Batsch) has been initiated fifteen years ago. Several sources of resistance are available among wild and domesticated peaches. Because all resistance genitors used in these programmes are of poor agronomic value, improvement of fruit quality is a long-lasting process. In order to avoid the erosion of the level of plant resistance during this process, the detailed analysis of the underlying mechanisms was undertaken. Here, we present some aspects of the work carried out on the genetic and physiological mechanisms of peach resistance to M. persicae. In addition to the analysis of the plant-aphid interaction under the influence of plant genetic factors, the effects of technical operations, such as winter pruning or nitrogen fertilization, on the aphid population dynamics are being investigated. Varietal resistance combined to cultural practices can be considered as an ultimate strategy to increase the efficacy of aphid control in orchards.
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titre
Host factors recruited by plant viruses during the infection process
auteur
Valerie Nicaise, Sylvie S. German-Retana, Bénédicte Doublet, Thierry Michon, Genevieve Roudet, Jocelyne J. Walter, Carole Caranta, Olivier Le Gall
article
APS Annual Meeting, Jul 2006, Québec, Canada. ⟨10.1094/PHYTO.2006.96.6.S1⟩
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Host factors recruited by plant viruses during the infection process
auteur
Valerie Nicaise, Sylvie S. German-Retana, Bénédicte Doublet, Thierry Michon, Genevieve Roudet, Jocelyne J. Walter, Carole Caranta, Olivier Le Gall
article
EMBO Workshop, Jul 2006, Helsinki, Finland
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Iconography of the Solanaceae from antiquity to the XVIIth century a rich source of information on genetic diversity and uses
auteur
M-Christin Daunay, Henri Laterrot, Jules Janick
article
6. International Solanaceae Conference, Jun 2006, Madison, United States
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Plants, Solanaceae and Solanum species: the lifelong passions of Richard Neville Lester (13 june 1937- 4 april 2006)
auteur
M-Christin Daunay, Gerard van Der Weerden
article
6. International Solanaceae Conference, Jun 2006, Madison, United States
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Development of an image database of Solanaceae
auteur
Jules Janick, Anna Whipkey, Harry Paris, Evelyne Jullian, M-Christin Daunay
article
6. International Solanaceae Conference, Jun 2006, Madison, United States
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Application of molecular markers in breeding for resistance to the Plum pox virus in Prunus spp
auteur
Ophélie Sicard, J.M. Soriano, Grégoire Marandel, D.A. Lalli, Patrick Lambert, M.L. Badenes, A.G. Abbott, Thierry Candresse, Olivier Le Gall, Véronique Decroocq
article
20. International Symposium on Virus and Virus-like diseases of temperate fruit crops, May 2006, Antalya, Turkey
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titre
La diversité des espèces légumières
auteur
Patrick P. Rousselle, Claude Foury, Michel Pitrat
article
8. Colloque scientifique de la Société Nationale d'Horticulture, May 2006, Bourges, France
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Metabolic profiling of shoot apices infested by the peach-potato aphid in susceptible and resistant peach cultivars
auteur
Jean-Luc Poessel, Marie-Helene Sauge-Collet, Marie Noëlle Corre, Christel Renaud, Monique Gaudillère, Mickael M. Maucourt, Catherine Deborde, Claire Dufour, Michele Loonis, Jean Philippe Lacroze, Thierry Pascal, Annick Moing
article
Proceedings from the 4th International Plant Metabolomics Conference, Apr 2006, Berkshire, United Kingdom
resume
1H NMR and targeted HPLCs were used to analyse the response of peach (Prunus persica L. Batsch) to infestation by the peach-potato aphid (Myzus persicae Sulzer, PPA). The response was studied in “Rubira”, a red-leaf cultivar resistant to PPA (Pascal et al, 2002) that shows strong level of induced resistance two days after infestation (Sauge et al, 2002) and “GF305”, a susceptible cultivar. Growing shoot apices, that are the preferred settling and feeding site of PPA, were sampled 48 hours after infestation and analysed for their 1H NMR profiles and their levels of main primary and secondary metabolites. Whereas no obvious differences were detected between metabolic profiles of infested and non-infested plants in the “GF305” susceptible cultivar, clear-cut changes in the 1H NMR and HPLC profiles were observed in Rubira following infestation. Carbohydrates and most organic acids showed a marked decrease. Several amino acids, including lysine and branched-chain and aromatic amino acids, showed a large accumulation whereas levels of glutamine, proline and threonine were greatly reduced. Infestation of Rubira by PPA also triggered accumulation of secondary metabolites, including phenolic and cyanogenic compounds. This first metabolomic approach of plant responses to infestation by an aphid species provides new insights into the metabolic pathways affected by insect feeding. Hypotheses on the mechanisms involved in induced resistance are discussed.
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NEWVIR - New strategy to control RNA virus infections targeted on host factors required for virus life cycle
auteur
Carole Caranta, Sylvie S. German-Retana, Carine Charron, Jean-Luc J.-L. Gallois, Sandrine Ruffel, Pascale Sanchez, Jocelyne J. Walter, Bénédicte Doublet, Geneviève Tavert-Roudet, Valerie Nicaise, Thierry Michon, Olivier Le Gall, Marianne Mazier, Christophe Robaglia
article
Séminaire Génoplante, Apr 2006, Arles, France
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GRASP IN - A global Ralstonia solanacearun project and initiatives to explore and survey phylogenetic diversity and to revisit resistance sources in Solanaceae
auteur
Chih-Hung Lin, Gabriel Mahbou, Mounira Elbaz, Jaw-Fen Wang, Sylvie Priou, Caroline Guilbaud, Christian Boucher, Emmanuel Wicker, Franck Bertolla, Alain Palloix, André Moretti, Rémi Kahane, Marie-Christine Daunay, Alice Guidot, Philippe Prior
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7. Rencontres Plantes-Bactéries, Mar 2006, Aussois, France
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Influence du fonds génétique sur l'expression de gènes et de QTL de qualité du fruit chez la tomate aux niveaux macroscopiques et moléculaires
auteur
Mathilde M. Causse
article
Influence du fonds génétique sur l'expression de gènes ou de transgènes, Mar 2006, Paris, France
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Translation initiation factors: components required for the virus life cycle and targets for plant resistance
auteur
Carole Caranta
article
7. Rencontres Plantes-Bactéries, Mar 2006, Aussois, France
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Characterisation and influence of floral abortion in french apricot crop area
auteur
Jean-Michel J.-M. Legave, Jean-Claude Richard, Dominique D. Fournier
article
12. International Symposium on Apricot Culture and Decline, Feb 2006, Avignon, France
resume
In apricot, abortion of floral primordium through tissue necrosis (commonly called 'floral anomalies') can occur early and intensively from 'dormant' stage or later with necrosis limited to pistil at flowering stage. Under the climatic conditions of French Mediterranean growing area, some varieties are unfruitful or irregular due to a high susceptibility to floral abortion. Nevertheless, in the French breeding program focused on disease resistance and fruit quality, some susceptible varieties are necessarily used as genetic resource. Therefore investigations on floral abortion were performed during six successive years in Mediterranean regions more particularly. Results recorded from a large genetic variability in varions climatic conditions allowed to characterise floral abortion regarding different features: main factors învolved, relationship with tree architecture, occurrence of abortion during floral development, influence on annual and biennal fruit bearing
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Impacts du réchauffement climatique sur la phénologie et le potentiel de floraison des arbres fruitiers. Texte + diaporama
auteur
Jean-Michel J.-M. Legave, Nadine N. Brisson, Marjorie Domergue, M.E. Liennard, Bernard Baculat, Bernard Seguin, André Belouin, Jean-Claude Richard, Alain Blanc, Guy Clauzel, B. Lafarge, Jacques Claverie, C. Hilaire, V. Mathieu, Caroline Pinet, B. Moulon
article
Rencontre chercheurs-professionnels, Feb 2006, Avignon, France
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Analysis of the interaction melon-melon necrotic spot virus controlled by the recessive resistance gene Nsv
auteur
Christine Nieto, Monica Morales, Gisella Orjeda, Veronica Truniger, Michel M. Caboche, Catherine Dogimont, Jordi Garcia-Mas, Miguel Aranda, Abdelhafid A. Bendahmane
article
14. Plant and animal genome, Jan 2006, San Diego, United States. 1 p
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QTLs controlling fruit quality trait in tomato: stability of individual effect and epistasis
auteur
Jamila Chaïb, Laurent Lecomte, Michel Buret, Frederic F. Hospital, Mathilde M. Causse
article
14. Plant & Animal Genome, Jan 2006, San Diego, United States
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Genetic analysis of temperate fruit quality and health in the new ISAFRUIT European Project
auteur
Pere Arus, Jean Marc Audergon, Silviero Sansavini, Francois Laurens, Frank Dunemann, Charles Eric Durel, Eric van de Weg, Marc Lahaye, Elisabeth Dirlewanger, Yves Lespinasse
article
14. Plant & Animal Genome, Jan 2006, San Diego, United States
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Poster communications

titre
Les Ressources Génétiques Lactuca
auteur
Brigitte Maisonneuve, Anne Pesme-Glemin
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2006, Avignon, France. 2006
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Le Mildiou de la laitue
auteur
Brigitte Maisonneuve, Anne Pesme-Glemin
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2006, Avignon, France. 2006
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titre
Des Résistances au Bremia plus Durables
auteur
Brigitte Maisonneuve, Anne Pesme-Glemin
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2006, Avignon, France. 2006
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titre
La laitue
auteur
Brigitte Maisonneuve, Anne Pesme-Glemin
article
MIFFEL, salon Méditerranéen Interprofessionnel de la Filière Fruits Et Légumes, Oct 2006, Avignon, France. 2006
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titre
Epidémiologie du mildiou (Bremia lactucae) dans une culture de laitue associant une lignée résistante à une variété sensible
auteur
Brigitte B. Maisonneuve, Claude de Vallavieille-Pope, Michel Pitrat
article
6. Rencontres de Phytopathologie/Mycologie de la Société Française de Phytopathologie, Jan 2006, Aussois, France. , 1 p., 2006
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Reports

titre
Analyse de la résistance au watermelon mosaic virus et au cucumber mosaic virus chez Arabidopsis thaliana
auteur
Virginie Pacaly
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2006, 32 p
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Résistance aux bactérioses foliaires de la laitue. Recherche et étude de géniteurs de résistance dans le genre Lactuca
auteur
Elodie Gautheron
article
[Stage] Institut Supérieur d’Agriculture de Beauvais (ISAB), Beauvais, FRA. 2006, 40 p
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titre
Vers la mise au point d'un protocole d'extraction des protéines de parois de tomate pour l'étude de la texture du fruit
auteur
Caroline Callot
article
[Stage] Institut Universitaire Professionnalisé. Université de Picardie Jules Verne (UPJV), Amiens, FRA. 2006, 56 p
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2005

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titre
Effets de l'infestation sur le profil métabolique d'apex de variétés de pêcher sensibles et résistantes au puceron vert
auteur
Jean-Luc Poessel, Marie-Hélène Sauge, Marie Noëlle Corre, Catherine Deborde, Claire Dufour, Christel Renaud, Monique Gaudillère, Mickael M. Maucourt, Michele Loonis, Jean Philippe Lacroze, Thierry Pascal, Annick Moing
article
1. Réseau français de métabolomique et fluxomique, Dec 2005, Toulouse, France. 1 p
resume
Les bases fonctionnelles des interactions entre plante et insectes piqueurs suceurs tels les pucerons restent mal connues malgré l’impact de ces ravageurs en agriculture. Dans le cadre de la caractérisation fonctionnelle de l’interaction entre le puceron Myzus persicae, espèce modèle en écologie, et son hôte primaire, le pêcher (Prunus persica L. Batsch), espèce modèle pour l’étude des Rosacées, nous avons mis en oeuvre une démarche d’analyse du métabolome sur les apex de pêcher, site d’alimentation privilégié de l’insecte. Nous avons étudié, par spectroscopie de RMN du proton et analyses HPLC ciblées sur les principaux pools de métabolites primaires et secondaires hydrosolubles, les modifications de profil métabolique induites par l’infestation dans les apex de deux variétés de pêcher, sensible (GF305) ou résistante (Rubira) à M. persicae [1,2]. L’analyse en composante principale de la signature spectrale obtenue par RMN et les analyses en HPLC révèlent de profondes modifications des profils métaboliques chez la variété résistante 48 heures après l’infestation : on observe notamment une baisse de la teneur de la plupart des sucres et acides organiques, de certains acides aminés (glutamine, proline, thréonine) alors que les teneurs en acides aminés ramifiés (leucine, isoleucine, valine) et aromatiques (phénylalanine, tyrosine) et en métabolites secondaires (composés phénoliques et cyanogéniques) augmentent. Les modifications observées chez la variété sensible sont faibles et pour la plupart divergent de celles observées chez la variété résistante. Ces résultats permettent d’émettre des hypothèses sur les voies métaboliques impliquées dans la réponse à l’infestation et sur la nature des effecteurs pouvant jouer un rôle dans la résistance induite chez Rubira. Cette étude met en lumière l’apport de l’approche métabolomique pour l’étude des interactions plante – insectes piqueurs suceurs.
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titre
Rétrotransposons comme outils d'analyse de la diversité chez la tomate et le poivron/piment
auteur
Marie Angele M. A. Grandbastien, Sheh May Tam, Mathilde M. Causse, Véronique Lefebvre, Alain Palloix, Aat Vogelaar, Corinne C. Mhiri
article
Les légumes. Un patrimoine à transmettre et à valoriser, Oct 2005, Angers, France
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titre
Origine et histoire de nos légumes
auteur
Michel Pitrat
article
Les légumes : un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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titre
Le réseau des ressources génétiques du melon
auteur
Michel Pitrat, Didier Besombes, J.P. Bouchet
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Avantages et inconvénients des différents modes de gestion des ressources génétiques de légumes existant en Europe
auteur
M.C. Daunay
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Evaluation de quelques critères de qualité sur trois groupes de tomate de frais (Solanum esculentum)
auteur
Helene Burck, Marie Blachier, Sophie Mirabel, Mathilde M. Causse, Patrick P. Rousselle
article
Les légumes un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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titre
Diversité des acteurs et des modes de conservation des espèces légumières: pré-enquête au travers du cas de la tomate
auteur
N. Perrot, Christian Deverre, Stephane Bellon, M.C. Daunay
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Le réseau des ressources génétiques du melon
auteur
Michel Pitrat, Didier Besombes, Jean-Paul Bouchet, - Sélectionneurs Privés
article
Les légumes Un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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Evaluation de quelques critères de qualité sur trois groupes de tomate de frais (Solanum esculentum)
auteur
Helène Burck, M. Blachier, S. Mirabel, Mathilde M. Causse, Patrick Rousselle
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Diversité des ressources génétiques chez le piment (Capsicum sp.) au sein de la collection INRA
auteur
A.M. Sage-Daubèze, Ghislaine Nemouchi, Therese Phaly, Alain Palloix
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Exemple d'évaluation des ressources génétiques du melon pour les résistances
auteur
Michel Pitrat, Didier Besombes, Hervé Lecoq, - Sélectionneurs Privés
article
Les légumes un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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titre
Le réseau français "ressources génétiques des solanacées à graines : aubergine, piment, tomate
auteur
M-Christin Daunay, Ingrid Mazal, Evelyne Jullian, Anne-Marie Daubèze, Therese Phaly, Francoise Jourdan, Helene Burck
article
Les légumes un patrimoine à transmettre, Sep 2005, Angers, France
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titre
Le réseau français Ressources génétiques des solanacées à graines: aubergine, piment, tomate
auteur
M.C. Daunay, I. Mazal, Evelyne Jullian, A.M. Daubèze, Therese Phaly, F. Jourdan, Helène Burck
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Diversité des ressources génétiques chez le Piment (Capsicum sp.) au sein de la collection INRA
auteur
Anne-Marie Sage-Daubèze, Ghislaine Nemouchi, Therese Phaly, Alain Palloix
article
Les légumes : un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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titre
Origine et histoire de nos légumes
auteur
Michel Pitrat
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Retrotransposons comme outils d'analyse de la diversité chez la tomate et le poivron/piment
auteur
Marie Angele M. A. Grandbastien, S.M. Tam, Mathilde M. Causse, Véronique Lefebvre, Alain Palloix, A. Vogelaar, Corinne C. Mhiri
article
Les légumes: un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France. 1 p
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titre
Réflexions sur la qualité, avec une référence particulière à l'aubergine
auteur
M-Christin Daunay
article
Les légumes un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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titre
Avantages et inconvénients des différents modes de gestion des ressources génétiques de légumes existant en Europe
auteur
M-Christin Daunay
article
Les légumes un patrimoine à transmettre et à valoriser, Sep 2005, Angers, France
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titre
Intérêt des espèces sauvages de Lactuca comme source de résistance à des bioagresseurs pour la laitue
auteur
Brigitte B. Maisonneuve
article
Les légumes : un patrimoine à transmettre et à valoriser, Association Française pour la Conservation des Espèces Végétales (AFCEV). FRA. Institut National d'Horticulture (INH), FRA. Bureau des Ressources Génétiques (BRG), FRA., Sep 2005, Angers, France. 1 p
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CGIS: an information system used for designing primers of candidate genes using Arabidopsis whole genome and Solanaceae EST databases
auteur
Cécile Bres, Jean-Paul Bouchet, L. Nucci, Bernard Caromel, Véronique Lefebvre
article
16. Triennal Conference of the EAPR, Jul 2005, Bilbao, Spain
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The synergic action of two resistance QTls originating from Solanum sparsipilum is required to enclose Globodera pallida juveniles in a necrosis
auteur
Bernard Caromel, Didier Mugniery, Marie-Claire Kerlan, Nadia Lama, Jean-Paul Dantec, Claudia Rouaux, Daniel Ellisseche, Françoise Rousselle-Bourgeois, Véronique Lefebvre
article
16. Triennal Conference of the EAPR, Jul 2005, Bilbao, Spain
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CDNA-AFLP screening for candidate genes differentially expressed in a Solanum clone resistant to Globodera pallida
auteur
K. Jolivet, Eric Grenier, Didier Mugniery, Magali Esquibet, Didier Fouville, Claudia Rouaux, Laura Chauvin, Daniel Ellissèche, Marie-Claire Kerlan, J.P. Dantec, Bernard Caromel, J.P. Bouchet, Nadia Lama, Véronique Lefebvre
article
16. Triennal Conference of the EAPR, Jul 2005, Bilbao, Spain. 1 p
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Update on french caribbean safeguarding invasive species CIRAD-INRA initiatives
auteur
Claude Vuillaume, Pierre-Yves Teycheney, Michel Dollet, Jean Daugrois, Claude Pavis, Alain Palloix, Cica Urbino, Christian Lavigne, Thierry Goguey, Dominique Martinez, Thierry Lefrançois, Sophie Molia
article
41. Annual meeting CFCS, Jul 2005, Le Gosier, Guadeloupe, France
resume
Cinq réseaux de recherche font actuellement l'objet de projets de constitution d'un réseau associatif de chercheurs caraïbéens dans le domaine de la sécurité sanitaire et des maladies émergentes et en progression dans la Caraïbe : la maladie à bégomovirus de la tomate, le jaunissement mortel du cocotier, les maladies des agrumes (tristeza, chancre citrique, CVC et greening), les maladies de la canne à sucre et les cercos- porioses et de BSV du bananier. Les premières missions de prises de contact et de finalisation de rédaction des projets de réseaux des partenaires débuteront en mai 2005 dans le cadre d'un projet global financé par l'Etat français et le Conseil Régional de Guadeloupe. Des analyses de risques phytosanitaires ont été réalisées en 2003 et 2004 par le Cirad en relation avec la Mission de Coopération Phytosanitaire du Ministère de l'Agriculture, de l'alimentation, de la Pêche et de la Ruralité. Un sixième réseau fonctionne depuis 1999, il inclut les services vétérinaires, les laboratoires de diagnostic, les universités, les partenaires nationaux et internationaux de la surveillance de la santé animale. Dans le cadre des activités de ce réseau, des missions d'appui technique et de diagnostic auprès des Etats de l'OECS ont débuté en 2004 et se poursuivront en 2005 principalement sur financement de l'ambassade de France.
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titre
Activités de recherche relatives aux pathosystèmes Solanacées / Phytophthora à l'UGAFL de Montfavet-Avignon
auteur
Véronique Lefebvre, . Inra
article
Réunion Oomycètes, Jun 2005, Clermont-Ferrand, France
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titre
Epidémiologie de Bremia lactucae dans des cultures de laitues sous abri
auteur
Brigitte B. Maisonneuve, G. Baudhuin, E. Martin, C. de Valavieille-Pope, Michel Pitrat
article
Réunion Oomycètes, Societe Francaise de Phytopathologie (SFP). FRA., Jun 2005, Clermont-Ferrand, France
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Application of Fourier-transform infrared (FT-IR) spectroscopy for the evaluation of quality traits in apricot fruits
auteur
S. Bureau, M. Reich, J.M. Audergon, Guy Albagnac
article
13. International Symposium on Apricot breeding and culture, Jun 2005, Murcie, Spain. 1 p
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QTL analysis of PPV (sharka) resistance in an F1 apricot "Polonais" x "Stark early orangé" progeny. Cross validation under Marcus and Dideron PPV infection strains
auteur
Patrick Lambert, F. Dicenta, I. Karayiannis, M. Rubio, Jean Marc Audergon
article
13. International symposium on apricot breeding and culture, Jun 2005, Murcia, Spain. 1 p
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titre
Application of Fourier-transform infrared (FT-IR) spectroscopy for the evaluation of quality traits in apricot fruits
auteur
Sylvie Bureau, Maryse Reich, C. Marfisi, Jean Marc Audergon, Guy Albagnac
article
13. International Symposium, Jun 2005, Murcia, Spain
resume
This work reports the first results concerning the rapid determination of quality traits of apricot using near (NIR) and middle (MIR) Fourier transform infrared reflectance spectroscopy and multivariate analysis. The FT-NIR spectra were acquired in duplicate on intact fruits using an integrating sphere (diffuse reflectance). A good prediction was obtained for soluble solids content and titratable acidity. The FT-MIR spectra were acquired for fruit purees using a horizontal ATR cell (Attenuated Total Reflectance). The prediction of sucrose, glucose, fructose and malic and citric acid contents was good.
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titre
Prospection, charaterization and assessment of apricot genetic resources in the mediterranean region for the production in arid and semi-arid areas
auteur
M.J. Asins, R. Guerriero, R. Viti, P. Monteleone, S. Bartolini, M. Laghezali, Aurélia Mamouni, M. Mlika, R. Hellali, Lamia Krichen, M. Ben Mimoun, R. Gulcan, S. Paydas, Guy Albagnac, S. Bureau, Barbara Gouble, Marie Grotte, M. Reich, Jean-Michel J.-M. Legave, Guy Clauzel, P. Lambert, A. Blanc, A.M. Ferréol, J.C. Richard, Veronique Signoret, J.M. Audergon, . Iamz
article
13. International Symposium on apricot breeding and culture, Jun 2005, Murcia, Spain. 1 p
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Comparison between ripening on trees and post-harvest evolution for apricot cultivars contrasting in their rate of ethylene production
auteur
Barbara Gouble, Marie Grotte, Patrice Reling, Maryse Reich, Sylvie Bureau, Guy Clauzel, Jean Marc Audergon, Guy Albagnac
article
13. International Symposium, Jun 2005, Murcia, Spain
resume
Fruit elaboration in orchards and post-harvest evolution are amongst the key factors to be kept under control in order to ensure fruit quality for the consumers. Due to the large variability already described in apricot ethylene production rate, we have focused on the comparison of contrasting apricot cultivars. For four apricot varieties (‘Moniqui’, ‘Goldrich’, ‘Bergeron’ and ‘A3759’), post-harvest storage at 23°C has been compared with fruit ripening on the tree, for three main traits: ethylene production, fruit firmness and colour changes. An earlier and higher ethylene production rate, associated with a faster loss of firmness, was observed post-harvest, in comparison with the fruit evolution on the tree. Colour changes also have been noticed. Differences amongst cultivars have been highlighted clearly.
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Variability, organisation and identification of Tunisian apricot (Prunus armeniaca l.) cultivars using AFLP markers
auteur
Lamia Krichen, N. Trifi-Farah, M. Marrakchi, Patrick Lambert, Jean Marc Audergon
article
13. International Symposium, Jun 2005, Murcia, Spain
resume
Widely adapted in most of the temperate areas in the world, apricot can be found in Tunisia in many contrasting zones, exhibiting a large variability, both from the climatic and the ecological points of view. In order to assess the genetic variability of this species, surveys were conducted in several areas of the country. Trees which were propagated by grafting and by seed were sampled. A total of 181 accessions were collected and characterised using AFLP markers. DNA extracted from young leaves was amplified with seven EcoRI-MseI AFLP primer combinations. Autoradiographs revealed a set of 303 polymorphic markers from the total of 398 detected bands, representing a high average polymorphism. Hierarchical clustering (UPGMA) revealed a large genetic diversity. It enabled the identification of several subgroups characterised by their earliness, type of fruit, type of propagation and geographic origin. It also allowed the resolution of some cases of homonymy and synonymy.
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Optimisation of the apricot improvement by a join conventional and molecular approach applied to the main agronomic traits ABRIGEN
auteur
J.M. Audergon, P. Lambert, A. Blanc, F. Gilles, J.C. Richard, Guy Albagnac, S. Bureau, Marie Grotte, Barbara Gouble, M. Reich, Jean-Michel J.-M. Legave, Guy Clauzel, F. Dicenta, R. Guerriero, R. Viti, P. Monteleone, S. Bartolini, M. Scortichini, A.M. Simeone, J.M.S. Martins, P. Psallidas, J. Tsiantos
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13. International Symposium on apricot breeding and culture, Jun 2005, Murcia, Spain. 1 p
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Kinetics of apricot fruit ethylene production in the orchard and post-harvest: a mathematical modelling approach
auteur
Marie Grotte, Barbara Gouble, Patrice Reling, Guy Clauzel, Jean Marc Audergon
article
13. International Symposium, Jun 2005, Murcia, Spain
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QTL analysis for resistance to sharka disease (PPV-Dideron strain) in apricot using an F1 progeny derived from ´Stark Early Orange´
auteur
Patrick Lambert, Jean Marc Audergon, F. Dicenta, M. Rubio
article
13. International Symposium, Jun 2005, Murcia, Spain
resume
Since the late 1980s, sharka disease has become a serious threat for apricot (Prunus armeniaca). It is caused by an aphid-borne virus, Plum pox virus (PPV), which is included in the genus Potyvirus. Two major types of PPV occur: Dideron (PPV-D) and Marcus (PPV-M) strains for which the only known sources of resistance in apricot have been identified in North-American cultivars. As traditional breeding methods are time-consuming, the identification of the regions of the genome involved in resistance to PPV is a promising solution for the selection of new resistant cultivars. For this purpose, phenotypic evaluations were carried out in Spain, in a greenhouse, during 4 vegetative cycles, using PPV-Dideron RB 3.30 isolate, on a progeny derived from a cross between the susceptible cv. ‘Polonais’ and ‘Stark Early Orange’, a resistant cultivar. Quantitative trait loci (QTL) analyses were then performed with QTL Cartographer software, using different statistical procedures. Three genomic regions involved in PPV resistance were detected. One of these mapped to the upper distal region of linkage group 1 of ‘Stark Early Orange’ and accounted for 56% of the phenotypic variation. Two additional regions accounting for minor effects were detected on 2 homologous linkage groups of the maps of both cultivars. PPV resistance seems to be controlled by one major dominant factor and putative additional recessive factors are suspected.
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Relationship and genetic variability of cell expansion and polyploidy during tomato pericarp development
auteur
Catherine Cheniclet, W.Y. Rong, Mathilde M. Causse, Nathalie Frangne, Jean-Pierre Renaudin
article
6. Colloque national de la SFBV, Apr 2005, Arcachon, France
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La résistance totale du piment au pepper veinal mottle virus résulte de la combinaison de mutations dans les gènes eIF4E1 et eIF(iso)4E. Communication orale
auteur
Sandrine Ruffel, Jean-Luc J.-L. Gallois, Carine Charron, Benoît Moury, Alain Palloix, Carole Caranta
article
10. Rencontres, Mar 2005, Aussois, France
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titre
Modifications de la régulation du génome de plante consécutives à l'infection par un virus: étude comparative entre la tomate et Arabidopsis thaliana
auteur
Xu Ping Feng, Maud M. Rivard, Johann J. Petit, Ludivine Taconnat, Christophe Rothan, Jean-Pierre J.-P. Renou, Valerie Schurdi-Levraud, Olivier Le Gall, Carole Caranta, Frederic Revers
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10. Rencontres de virologie végétale, Mar 2005, Aussois, France
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Production d'un sérum polyclonal spécifique du parietaria mottle ilarvirus (PMoV), et étude de sa transmission par le pollen. Poster
auteur
Eric Verdin, Patrick Gognalons, Loïc Cardin, André Moretti, Giuseppe Parrella, Anne-Cécile Cotillon, Mireille Jacquemond, Georges Marchoux
article
10. Rencontres, Mar 2005, Aussois, France
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titre
La résistance quantitative au virus de la sharka chez les Prunus : premiers résultats de la validation génétique et fonctionnelle de gènes candidats impliqués dans l'interaction Prunus / PPV
auteur
Véronique Decroocq, Valerie Schurdi-Levraud, Marie Foulongne, Ophélie Sicard, Magali Eychenne, E. Matthieu, Jean-Philippe Eyquard, Patrick Lambert, Thierry Pascal, D. Abernathy, C. Romero, M.L. Badenes, Olivier Le Gall
article
10. Rencontres de virologie végétale, Mar 2005, Aussois, France. 1 p
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Plusieurs substitutions ponctuelles chez le potato virus Y (PVY, potyvirus) permettent indépendamment le contournement d'une résistance monogénique chez le piment. Communication orale
auteur
Valérie Ayme, Sylvie Souche, Alain Palloix, Carole Caranta, Mireille Jacquemond, Benoît Moury
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10. Rencontres, Mar 2005, Aussois, France
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Role of eIF4E gene redundancy in plant resistance to viruses
auteur
Jean-Luc J.-L. Gallois, Carine Charron, Sandrine Ruffel, Christophe Robaglia, Catherine Dogimont, Carole Caranta
article
10. Rencontres, Mar 2005, Aussois, France
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titre
Modélisation de l'élaboration des performances agronomiques sous l'influence du milieu, des techniques, du génotype. La qualité des pêches et sa variabilité. Diaporama
auteur
Francoise F. Lescourret, Claude Bussi, Bénédicte Quilot
article
Production Fruitière Intégrée, Jan 2005, Paris, France. 10 p
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Efficiency of marker-assisted selection for organoleptic quality in tomato
auteur
Mathilde M. Causse, Jamila Chaïb, Laurent Lecomte, Frederic F. Hospital, Michel Buret
article
13. Conference Plant and Animal Genomes, Jan 2005, San Diego, United States
resume
In order to help breeders in their attempt at improving organoleptic quality of fresh market tomato a QTL mapping experiment was performed, followed by marker-assisted breeding. The detection of QTLs controlling the variation of several fruit quality traits was performed in the progeny of a cross between a cherry tomato chosen for its good flavour and a line with bigger but less tasty fruits. The lines were evaluated for physical, chemical and sensorial traits. QTLs were detected for all the traits and 8 major clusters of QTLs were detected. The favourable alleles for quality traits came from the cherry tomato, showing the potential usefulness of this line for organoleptic quality improvement. On the contrary, this line provided smaller fruits at all the fruit weight QTLs. A marker-assisted selection scheme was thus set up to transfer into elite lines the five regions carrying the most important QTLs for fruit quality. A backcross scheme was optimised taking into account both theoretical aspects and practical constraints. Three recurrent lines were chosen in order to study the effect of genetic background on QTL expression. After three backcrosses and two selfing generations, lines carrying one to five introgressed regions were selected and evaluated. About half of the QTLs appeared to be stable over genetic backgrounds and generations. A positive improvement of quality components was observed, but fruit weight could not be recovered as expected. Several epistatic interactions among QTLs were shown, which limit the possible progress.
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The International Solanaceae Genome Initiative (SOL): Biodiversity of scientists and plants
auteur
Mathilde M. Causse, . International Solanaceae Genome Project Community
article
13. Conference Plant & Animal Genomes, Jan 2005, San Diego, United States
resume
The “International Solanaceae Genome Project (SOL): Systems Approach to Diversity and Adaptation” was initiated in November 2003. The goal of this grassroots project are to establish a network of information, resources and scientists to tackle two of the most significant questions in plant biology and agriculture: 1- How can a common set of genes/proteins give rise to a wide range of morphologically and ecologically distinct organisms that occupy our planet? 2- How a deeper understanding of genetic basis of plant diversity can be harnessed to better meet the needs of society in an environmentally-friendly and sustainable manner? The Solanaceae family is ideally suited to address both of these questions. It includes more than 3000 species adapted to a whole range of habitat and climate. Solanaceae crops have been intensively selected by man, allowing their use as models to study the evolutionary interface between plants and people. The unique and ancient mode of conservation of genome organization at the macro and micro levels, makes the family a unique subject to explore the basis of phenotypic diversity, evolution of gene function and adaptation to natural and agricultural environments. In this overview I will describe the start of common journey of scientists from 30 different countries into unexplored biodiversity
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Recherche de résistances à Xanthomonas campestris pv. vitians, agent responsable de bactériose foliaire chez la laitue
auteur
Brigitte B. Maisonneuve, C. Chassagne
article
Rencontres, Jan 2005, Angers, France
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https://hal.inrae.fr/hal-02760135/file/Maisonneuve-rencCTIFL-janv05-Angers-Xantho_1.pdf BibTex
titre
New detection of QTLs controlling major fruit quality components in peach (résumé)
auteur
Elisabeth Dirlewanger, Patrick Cosson, Christel Renaud, Jean-Luc Poessel, R. Monet, Annick Moing
article
6. International Peach Symposium, Jan 2005, Santiago, Chile
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Combining ecophysiological and genetic models to analyse peach organoleptic quality
auteur
Bénédicte Quilot-Turion, J. Kervella, Thierry Pascal, Michel M. Génard, Francoise F. Lescourret, B.H. Wu
article
6. International peach symposium, Jan 2005, Santiago du Chili, Chile
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Poster communications

titre
En quoi l'étude de l'épidémiologie des maladies à bégomovirus de la tomate peut-elle aider à la conception de systèmes de culture innovants ?
auteur
Claude Pavis, Cica Urbino, Maure Rousseau, David Lange, Marie Robin, Albert Huc, Raphaelle Gotin, Nathalie Boissot
article
41. Annual ùmeeting CFCS, Jul 2005, Le Gosier, Guadeloupe, France. Caribbean Food Crops Society, Caribbean Food Crops Society Proceedings, 41 (n° 2), 601 p., 2005, Proceedings of the 41st annual meeting "Alternatives to high input agriculture in the Caribbean: towards the elaboration on innovative systems"
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Epidémiologie des maladies à begomovirus en Guadeloupe
auteur
Claude Pavis, Cica Urbino, M. Marquier, J. Agrapart, Marie Robin, Maure Rousseau, David Lange, Nathalie Boissot
article
10. Rencontres de virologie végétale, Mar 2005, Aussois, France. 1 p., 2005
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Reports

titre
Le nombre de loges chez la tomate: vers le clonage d'un QTL
auteur
David Tricon
article
[Stage] Faculté des Sciences de St Jérôme. Université de Provence (Aix Marseille 1), Marseille, FRA. 2005, 40 p
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Etude du déterminisme génétique et physiologique de la coloration rouge chez l'abricotier et validation d'un test précoce de la pigmentation rouge
auteur
A. Mathé
article
[Stage] Institut National Polytechnique (INP), Toulouse, FRA.; Ecole Nationale Supérieure Agronomique (ENSA), Toulouse, FRA. 2005, 44 p
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Biologie moléculaire et bioinformatique dans le cadre du projet Apophys. Hybridation de produits d'amplification PCR spécifiques de gènes candidats sur filtres haute-densité et réalisation de l'interface web de consultation du système d'information des gènes candidats
auteur
S. Coumes
article
[Stage] IUT Génie Biologique. Université de Provence (Aix Marseille 1), Digne les Bains, FRA. 2005, 90 p
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Validation fonctionnelle de gènes de résistance récessive chez la tomate et le melon
auteur
Amira Youssef
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), FRA.; Ecole Nationale Supérieure Agronomique (ENSA), Montpellier, FRA. 2005, 32 p
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Cartographie génétique fine d'un locus à effet quantitatif sur la résistance aux Phytophthora spp. chez le piment
auteur
M. Kerneïs
article
[Stage] Faculté des Sciences de Luminy. Université Paul Cézanne (Aix Marseille 3), Marseille, FRA. 2005, 37 p
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2004

Conference papers

titre
Peach fruit quality : from QTLs to Rosaceae synteny
auteur
Elisabeth Dirlewanger, Patrick Cosson, Cédric Renaud, Jean-Luc Poessel, R. Monet, Annick Moing
article
France Israël Agro-Biotechnologies Workshop, Dec 2004, Auzeville, France
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Amino acid diversity at the translation initiation factor 4E (eIF4E) involved in recessive resistance to several plant viruses
auteur
Carole Caranta, Catherine Dogimont, Sandrine Ruffel, Valerie Nicaise, Olivier Le Gall, Véronique Decroocq
article
Séminaire, Dec 2004, Paris, France
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Génomique fonctionnelle chez l'abricotier
auteur
Jérôme Grimplet, Charles Romieu, Nancy Terrier, S. Bureau, Marie Grotte, Barbara Gouble, Isabelle Marty, Guy Albagnac, J.M. Audergon, J.P. Bouchet, P. Lambert
article
Séminaire, Dec 2004, Paris, France
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Biotechnologies in horticulture (vegetables, ornamentals, fruits, medicinal and aromatic plants), a survey of techniques and examples
auteur
M.C. Daunay, J. Boccon-Gibod, Alain Cadic, Elisabeth E. Chevreau, Noëlle Dorion, Daniel Ellissèche, Marianne Mazier, J.N. Plages
article
8. Symposium, Nov 2004, Velika Plana
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titre
Screening of genes involved in textural changes in fruit using texture-targeted micro-array analysis on tomato NILs
auteur
Damien Sudre, L. Tichit, David Page, Mathilde M. Causse, Isabelle Marty
article
3. Plant GEMS, Sep 2004, Lyon, France
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titre
Map-based cloning of the gene Vat from melon, which confers resistance to both aphid colonization and aphid transmission of several viruses
auteur
J. Pauquet, E. Burget, Lynda Hagen, Veronique Chovelon, Aline Le Menn, N. Valot, Sophie Desloire, Michel M. Caboche, Patrick Rousselle, Michel Pitrat, Abdelhafid Bendahmane, Catherine Dogimont
article
3. Plant GEMS, Sep 2004, Lyon, France
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titre
Suppression subtractive hybridization using QTL-near isogenic lines of tomato to identify transcripts involved in fruit quality traits
auteur
David Page, Mathilde M. Causse, L. Tichit, Isabelle Marty
article
3. Plant GEMS, Sep 2004, Lyon, France
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titre
Positional cloning of a recessive resistance gene to the melon necrotic spot carmovirus and plant-virus interactions implicated in the resistance
auteur
G. Orjeda, Catherine Dogimont, Christine Nieto, M. Morales, J. Garcia, M. Aranda, Abdelhafid Bendahmane
article
3. Plant GEMS, Sep 2004, Lyon, France
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titre
Plant resistance against potyvirus: a subtle interplay between the eukaryotic translation initiation factor eIF4E and viral-encoded proteins
auteur
Jean-Luc J.-L. Gallois, Sandrine Ruffel, Benoît Moury, Alain Palloix, Christophe Robaglia, K. Browning, Carole Caranta
article
3. Plant GEMS, Sep 2004, Lyon, France
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titre
Fine-mapping the Phy-P5 QTL involved in the resistance to Phytophthora in pepper and anchorage with the ultra-high density potato map
auteur
Véronique Lefebvre, Sarah Danan, J. Bonnet, C. Boudet, M. Hédont, Bernard Caromel, A.M. Daubèze, Veronique Signoret, Anne Blattes, Therese Phaly, Alain Palloix
article
3. Plant GEMS, Sep 2004, Lyon, France
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titre
Integrated approach of the characterization of tomato fruit quality
auteur
Jamila Chaïb, Stephane Munos, C. Mihr, Mireille Faurobert, Nadia Bertin, Isabelle Marty, Mathilde M. Causse
article
3. Plant GEMS, Sep 2004, Lyon, France
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Solanaceae genetic resources in Europe : the ECP/GR network
auteur
M-Christin Daunay, W. Dooijeweert, Lorenzo Maggioni
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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titre
Genetic control of tomato fruit quality traits
auteur
Mathilde M. Causse, Jamila Chaïb, Laurent Lecomte, Mireille Faurobert, Stephane Munos, Michel Buret, Isabelle Marty
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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Eggplant genetic resources network (EGGNET))
auteur
M-Christin Daunay, J.W. Hennart, G. van Der Weerden, G. Barendse, J. Haanstra, R.N. Lester, W. van Dooijeweert, J. Prohens, F. Bletsos, G. Polignano, A. Börner, J.Y. Peron
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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QTLs for resistance to the cyst nematode Globodera pallida, deriving from Solanum sparsipilum mapped in resistance gene clusters
auteur
Bernard Caromel, Didier Mugniery, Marie-Claire Kerlan, Daniel Ellisèche, S. Andrzejewski, Nadia Lama, Jean-Paul Dantec, Claudia Rouaux, Carole Caranta, Peggy Cavallin, Françoise Rousselle-Bourgeois, Véronique Lefebvre
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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titre
Use of molecular markers to detect clones bearing QTLs derived from Solanum sparsipilum and Solanum spegazzinii conferring resistance to potato cyst nematode Globodera pallida
auteur
Laura Chauvin, Marie-Claire Kerlan, Didier Mugniery, Jean-Paul Dantec, Josiane Quéré, Bernard Caromel, Véronique Lefebvre, Daniel Ellisèche
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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titre
Solanaceae as a model system for the molecular characterization of hosts factors required for potyvirus infection
auteur
Carole Caranta, Sandrine Ruffel, Jean-Luc J.-L. Gallois, Nicolas Fabre, Alain Palloix, Abdelhafid A. Bendahmane, Christophe Robaglia
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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titre
Screening genes involved in texture changes in fruit using texture-targeted micro-arrays on tomato lines
auteur
Damien Sudre, Line Tichit, David Page, Mathilde M. Causse, Isabelle Marty
article
1. Solanaceae Genome Workshop 2004, Sep 2004, Wageningen, Netherlands
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titre
Genetic mapping of quantitative trait loci involved in the resistance to plum pox virus in Prunus davidiana
auteur
Véronique Decroocq, Marie Foulongne, Patrick Lambert, Thierry Pascal, Valerie Schurdi-Levraud, Jocelyne Kervella, Olivier Le Gall
article
European Meeting '04 on plum pox, Sep 2004, Skierniewice, Poland. 1 p
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titre
Resistances to Bremia lactucae from Lactuca virosa; Research for a strategy for durable resistance
auteur
Brigitte Maisonneuve, E. Martin, C. de Vallavieille-Pope, Michel Pitrat
article
17. International lettuce and leafy vegetable conference, Aug 2004, Montreal, Canada
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titre
A virtual fruit to analyse the genetic variability of quality traits. Diaporama
auteur
Bénédicte Quilot-Turion, Michel M. Génard, J. Kervella, Francoise F. Lescourret
article
International workshop, Jul 2004, Clermont-Ferrand, France
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titre
Map-based cloning of the Vat gene from melon conferring resistance to both aphid colonization and aphid transmission of several viruses
auteur
J. Pauquet, E. Burget, Lynda Hagen, Veronique Chovelon, Aline Le Menn, N. Valot, Sophie Desloire, Michel M. Caboche, Patrick Rousselle, Michel Pitrat, Abdelhafid Bendahmane, Catherine Dogimont
article
8. EUCARPIA Meeting, Jul 2004, Olomouc, Czech Republic
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titre
Susceptibility to sulfur dusting and inheritance in melon
auteur
Laure Perchepied, Christophe Périn, Nathalie Giovinazzo, Didier Besombes, Catherine Dogimont, Michel Pitrat
article
8. EUCARPIA Meeting, Jul 2004, Olomouc, Czech Republic
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titre
A physical map covering the Nsv locus in melon
auteur
J. Garcia-Mas, M. Morales, H. van Leeuwen, A. Monfort, P. Puigdomenech, Pere Arus, Christine Nieto, Maria Aranda, Catherine Dogimont, G. Orjeda, Michel M. Caboche, Abdelhafid Bendahmane
article
8. EUCARPIA Meeting, Jul 2004, Olomouc, Czech Republic
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titre
Genetic analysis of resistance to Fusarium oxysporum F. sp. melonis race 1.2 in melon
auteur
Laure Perchepied, Catherine Dogimont, Michel Pitrat
article
8. EUCARPIA Meeting, Jul 2004, Olomouc, Czech Republic
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titre
Molecular mapping of the melon Fom-1/prv locus
auteur
Y. Brotman, I. Kovalski, Catherine Dogimont, Michel Pitrat, N. Katzir, R. Perl-Treves
article
8. EUCARPIA Meeting, Jul 2004, Olomouc, Czech Republic
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titre
Comparison of plant leaf area, light interception efficiency, plant photosynthesis and crop yield of tomato lines of contrasted architecture
auteur
Hélène Gautier, Hervé Sinoquet, V. Douzon, J.C. L'Hotel, C. Polizzano, Mathilde Causse
article
4. International Workshop on functional-structural plant models, Jun 2004, Montpellier, France
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absent
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titre
Proteomics of genetic diversity in relation to tomato fruit size
auteur
T. Pawlowski, Marie Angele M. A. Grandbastien, Mireille Faurobert, C. Mihr, Mathilde M. Causse
article
5. International Symposium, Jun 2004, Verone, Italy
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titre
Apricot postharvest ability in relation to ethylene production: influence of picking time and cultivar
auteur
Barbara Gouble, S. Bureau, Marie Grotte, M. Reich, Patrice Reling, J.M. Audergon
article
5. International Symposium, Jun 2004, Verone, Italy
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titre
Proteome analysis of organoleptic quality in tomato
auteur
C. Mihr, Mireille Faurobert, J.P. Bouchet, Mathilde M. Causse, T. Pawlowski, Luc Négroni, M. Rossignol, M. Sommerer
article
5. International Symposium, Jun 2004, Verone, Italy
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Genetic analysis of major fruit quality components in peach. Comparison with apricot (résumé)
auteur
Elisabeth Dirlewanger, Patrick Cosson, Cédric Renaud, P. Lambert, J.M. Audergon, Bénédicte Quilot-Turion, Veronique Signoret, Jean-Luc Poessel, Abdelhafid Bendahmane, Annick Moing
article
2. International Rosaceae Genome Mapping Conference, May 2004, Clemson, United States
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EST in Prunus genus (apricot and peach)
auteur
Jérôme Grimplet, Charles Romieu, F.X. Sauvage, Nancy Terrier, P. Lambert, J.M. Audergon, Elisabeth Dirlewanger, Patrick Cosson, Loïck Le Dantec, Annick Moing
article
International rosaceae genome mapping conference, May 2004, Clemson, SC, United States
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titre
Heredity of tomato mosaic virus (ToMV) and potato virus Y (PVY) resistance in eggplant (Solanum melongena L.)
auteur
Marie-Christine Daunay, Carole Caranta, F. Pedron, Chrystel Deulvot, M.H. Dussault, André Moretti, Evelyne Jullian, Sandrine Ruffel, Frédéric Moquet, M. Majde, Kahsay Gebre Selassie, Patrick Gognalons, Georges Marchoux
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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Comparison of S. melongena MM738 and S. linnaeanum MM195 for salinity resistance
auteur
M.C. Daunay, Stephane Adamowicz, Jacques Le Bot, Josiane Hostalery, J. Fabre
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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Eggplant (Solanum melongena) fruit colour: pigments, measurements and genetics
auteur
M.C. Daunay, S. Aubert, A. Frary, S. Doganlar, R.N. Lester, G. Barendse, G. van Der Weerden, J.W. Hennart, J. Haanstra, F. Dauphin, Evelyne Jullian
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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PVY evolution toward virulence to the resistance conferred by pvr2-3 in pepper and consequences for its durability
auteur
Valérie Ayme, Olivier Pierrugues, Sylvie Souche, Ghislaine Nemouchi, Carole Caranta, Mireille Jacquemond, Joel J. Chadoeuf, Alain Palloix, Benoît Moury
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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Involvement of the eukaryotic translation initiation factor elF4E in Solanaceae-Potyvirus interactions
auteur
Sandrine Ruffel, M.H. Dussault, Marie-Laure Lesage, André Moretti, Alain Palloix, Marie-Christine Daunay, Benoît Moury, Abdelhafid Bendahmane, Christophe Robaglia, Carole Caranta
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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Results of three cycles of marker-assisted backcross in pepper for resistance QTLs to Phytophthora capsici
auteur
Véronique Lefebvre, A. Thabuis, Bertrand Servin, A.M. Daubèze, Patrick Signoret, Frederic F. Hospital, Alain Palloix
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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titre
Performance of phenotypic selection for a polygenic trait: QTL conservation and loss across the cycles of selection of pepper for resistance to Phytophthora capsici
auteur
Alain Palloix, G. Bernard, A. Thabuis, A.M. Daubèze, Therese Phaly, Patrick Signoret, Véronique Lefebvre
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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titre
Fine-mapping of a quantitative resistance locus to Phytophthora spp. and development of NIL-QTLs in pepper
auteur
Véronique Lefebvre, J. Bonnet, C. Boudet, M. Hédont, Patrick Signoret, Therese Phaly, Anne Blattes, Sarah Danan, Alain Palloix
article
12. Meeting on genetics and breeding of Capsicum and eggplant, May 2004, Noordwijkerhout, Netherlands
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titre
Genetic, cytological and molecular bases of the resistance to root-knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)
auteur
C. Djian-Caporalino, F. Berthou, Ariane Fazari, Véronique Lefebvre, Alain Palloix, Anthony Pegard, Lucette Pijarowski
article
12. EUCARPIA Meeting, May 2004, Noordwijk, Netherlands
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titre
Polymorphisme et localisation chez l'abricotier et le pêcher de gènes de résistance et de défense potentiellement impliqués dans la réponse de la plante au virus de la sharka
auteur
P. Lambert, C. Mantin, Véronique Decroocq, Marie Foulongne, V. Shurdi-Levraud, J. Kervella, J.M. Audergon
article
8. Journées, May 2004, Clermont-Ferrand, France
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Etude génétique de composantes majeures de la qualité organoleptique et nutritionnelle de la pêche
auteur
Elisabeth Dirlewanger, Patrick Cosson, Cédric Renaud, Jean-Luc Poessel, Frédéric Laigret, Annick Moing
article
8. Journées Biologie Moléculaire des Ligneux, May 2004, Clermont-Ferrand, France
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Identification of QTL involved in resistance to Hemiptera in Cucumis melo
auteur
Nathalie Boissot, C. Marchal, Nicolas Sauvion, Denis Lafortune, Virginie Chareyron, J.A. Huc, E. Etresse, Daniel Marival, Claude Pavis, Catherine Dogimont
article
16. Biennal international plant resistance to insects workshop, Mar 2004, Baton Rouge, United States. 1 p
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[Some characteristics of fruit and vegetable commodity systems]
auteur
Benoit B. Jeannequin, F. Dosba, Marie Josèphe Amiot, J.C. Lescourret, Jean-Claude Montigaud, Daniel Hassan, Dominique Blancard, Michel Pitrat, Yvonne Couteaudier
article
Salon international de l'agriculture, Feb 2004, Paris, France. 28 p
resume
Le document synthétique a été réalisé à partir d'une étude conduite sur le fonctionnement actuel des filières fruits et légumes par un groupe de chercheurs représentant différentes activités de recherche pour ce secteur. Il répond à la volonté de l'Inra d'établir les bases d'une culture commune et d'une vision partagée permettant un dialogue direct entre équipes de recherche mais aussi avec les professionnels. En analysant le fonctionnement de ces filières, ses contraintes, ses atouts et ses principaux enjeux, l'objectif de l'institut est de comprendre, et si possible entrevoir, les questions qui sont ou seront posées à ces filières. Cette action est la première étape d'une démarche de co-construction avec les acteurs des filières des problématiques de recherche que les auteurs choisisent collectivement de traiter. Par cette approche "partenariale", ils souhaitent créer une proximité plus grande entre chercheurs de l'Inra et professionnels des filières fruits et légumes pour élaborer des réponses collectives présentant des bénéfices partagés et réciproques.
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Amélioration de l'abricotier: état d'avancement et perspectives du programme conduit par l'INRA
auteur
J.M. Audergon, Guy Clauzel
article
Séminaire Gotheron, Jan 2004, St Marcel les Valence, France
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Hérédité de la résistance à l'oïdium et au mildiou dans une lignée de melon
auteur
Laure Perchepied, Marc Bardin, Michel Pitrat
article
5. Rencontres de Phytopathologie/Mycologie, Jan 2004, Aussois, France
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titre
Genetic and functional genomic analysis of organoleptic quality components in tomato fruit
auteur
Mireille Faurobert, C. Mihr, Stephane Munos, L. Lecomte, Mathilde M. Causse
article
12. Plant and animal genome, Jan 2004, San Diego, CA, United States
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Identification of molecular markers linked to Fusarium oxysporum fsp melonis race 1.2 in melon.
auteur
Laure Perchepied, Catherine Dogimont, Michel Pitrat
article
12. Plant and animal genome, Jan 2004, San Diego, CA, United States
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Poster communications

titre
Modification de la teneur en acides aminés des apex de pêcher en réponse à l'infestation par le puceron vert Myzus persicae (Sulzer) : rôle potentiel dans la résistance
auteur
Jean-Luc Poessel, M.N. Corre, Monique Gaudillère, M.H. Sauge, J.P. Lacroza, Cédric Renaud, Catherine Deborde, M. Maucourt, Thierry Pascal, Annick Moing
article
Ecole thématique "Réponses des plantes aux facteurs environnementaux abiotiques", Nov 2004, Montpellier, France. 1 p., 2004
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A new tool for gene tagging in lettuce (Lactuca sativa): the Tnt1 LTR-retrotransposon from tobacco
auteur
Marianne Mazier, Emmanuel Botton, E. Flamain, Béatrice Courtial, Verane Sarnette, M.C. Chupeau, Yves Y. Chupeau, Brigitte B. Maisonneuve, Helene Lucas
article
3. Plant GEMS, Sep 2004, Lyon, France. 2004, Plant genomics european meetings
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Pollen-mediated gene flow by insects in Lactuca sativa
auteur
Brigitte Maisonneuve, S. Valéry, Nicolas Morison, Bernard Vaissière
article
17. International lettuce and leafy vegetable conference, Aug 2004, Montréal, Canada. 2004, 17. International congress on lettuce and leafy vegetables
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titre
Identification of resistance to potato yellow mosaic virus (Guadeloupe) and Tomato yellow Mosaic Virus (Reunion) among some Lycopersicon species.
auteur
Hélène Delatte, Cica Urbino, Martial Grondin, Albert Huc, Nathalie Boissot, Bernard Reynaud, Jacques Dintinger
article
1st International symposium on tomato disease and 19th annual tomato disease worshop, Jun 2004, Orlando, United States. 2004
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titre
Phloem-mediated expression of peach resistance and susceptibility in response to feeding by the aphid Myzus persicae
auteur
M.H. Sauge, J.P. Lacroze, Thierry Pascal, J. Kervella, Jean-Luc Poessel, . Société Française de Biologie Végétale
article
Le phloème dans tous ses états, Apr 2004, Poitiers, France. 1 p., 2004
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Développement de Bremia lactucae sur une variété de laitues cultivée en association avec une résistance introgressée de Lactuca virosa
auteur
Brigitte B. Maisonneuve, E. Martin, C. de Valavieille-Pope, Michel Pitrat
article
5. Rencontres de Phytopathologie/Mycologie, Jan 2004, Aussois, France. 2004, Journées Jean Chevaugeon
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Reports

titre
Etude de l'influence du génotype et de la souche d'Agrobacterium tumefaciens sur les taux de régénération et de transformation de la tomate
auteur
Marion Prudent
article
[Stage] Institut National d'Horticulture (INH), Angers, FRA. 2004, 42 p
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titre
Recherche et étude de résistance aux bactérioses foliaires chez la laitue
auteur
C. Chassagne
article
[Stage] Ecole Nationale Supérieure Agronomique (ENSA), Toulouse, FRA. 2004, 32 p
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titre
Caractérisation par approche protéomique de QTL de qualité organoleptique chez la tomate Lycopersicon esculentum
auteur
David Tricon
article
[Stage] IUP Agrosciences. Université d'Avignon et des Pays de Vaucluse (UAPV), FRA. 2004, 56 p
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titre
Epidémiologie de Bremia lactucae dans des cultures de laitue en association
auteur
G. Baudhuin
article
[Stage] Ecole Nationale des Ingenieurs des Travaux Agricoles (ENITA), Lempdes, FRA.; Laboratoire de Physiologie et Génétique végétales. Université Clermont Auvergne (UCA), Aubière, FRA. 2004, 25 p
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titre
Recherche par la technique cDNA-AFLP de gènes impliqués dans l'interaction piment / Phytophthora capsici
auteur
A. Imbert-Fillols
article
[Stage] UFR Sciences exactes et de la nature. Université d'Avignon et des Pays de Vaucluse (UAPV), FRA. 2004, 31 p
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Mesure de l'efficacité de la résistance du melon au puceron Aphis gossypii conférée par le gène Vat, à l'état homozygote et hétérozygote, en culture de plein champ
auteur
C. Henry
article
[Stage] IUT Génie biologique. Université d'Avignon et des Pays de Vaucluse (UAPV), FRA. 2004, 20 p
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titre
Outil de consultation et de modification d'une base de ressources génétiques
auteur
G. Gagniard
article
[Stage] Institut d'Informatique d'Entreprise. Conservatoire National des Arts et Métiers [CNAM] (CNAM), Evry, FRA. 2004, 31 p
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titre
Traitement bio-informatique de gènes candidats impliqués dans les mécanismes de résistance aux bioagresseurs de la pomme de terre
auteur
L. Nucci
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), FRA. 2004, 45 p
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2003

Conference papers

titre
Obtencion y caracterizacion preliminar de plantas de tomate (Lycopersicon esculentum) transformadas con genes de capside de diferentes potyvirus
auteur
Miryam Valenzuela, Aline Le Menn, Patrick Rousselle
article
13. Congreso, Oct 2003, Marbella, Chile
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titre
Genetic relationships between peach (Prunus persica (L.) Batsch) and closely related wild species using SSR markers
auteur
Marie Foulongne, J. Minier, Thierry Pascal, J. Kervella
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Breeding for a new generation of Prunus rootstocks based on marker-assited selection : a European initiative
auteur
Elisabeth Dirlewanger, Marc M. Kleinhentz, Frédéric Laigret, J. Gomez-Aparisi, M.J. Rubio-Cabetas, Michel Claverie, Nathalie Bosselut, Roger R. Voisin, Daniel Esmenjaud, C. Xiloyannis, B. Dichio, Jean-Luc Poessel, M. Di Vito, P. Arus, W. Howad
article
11. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2003, Angers, France
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titre
Inheritance of organoleptic traits of apricot
auteur
Veronique Signoret, S. Bureau, M. Reich, Barbara Gouble, Guy Clauzel, Guy Albagnac, J.M. Audergon
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Agronomic evaluation of INRA Prunus rootstocks for two japanese plum cultivars: Friar and Black Amber
auteur
Henri Duval
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Comparative mapping of Prunus armeniaca, Prunus cerasifera x Prunus armeniaca and Prunus reference map
auteur
P. Lambert, Mireille Faurobert, Esther Pelpoir, J.L. Moreau, Jean-Luc Poessel, J.M. Audergon
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Use of Myrobolan species (Prunus cerasifera) as rootstocks for almond and peach
auteur
Henri Duval, Veronique Signoret, R. Joly
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Re-examination of an introgression procedure of a polygenic resistance to powdery mildew from wild Prunus davidiana into peach in the light of molecular data and Monte-Carlo simulations
auteur
J. Kervella, Marie Foulongne, Thierry Pascal, F. Pfeiffer, K. Moreau, P. Lambert
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Breeding for a new generation of root-knot nematode resistant prunus rootstock based on MAS: a european initiative
auteur
Elisabeth Dirlewanger, Marc Kleinhentz, Frédéric Laigret, M. Claverie, Nathalie Bosselut, Jean-Luc Poessel, Pere Arus, J. Gomez-Aparisi, M. Di Vito, C. Xiloyannis, Daniel Esmenjaud
article
Eucarpia symposium, Sep 2003, Angers, France
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titre
Inheritance of plum pox virus resistance in apricot
auteur
M. Rubio, P. Martínez-Gómez, J.M. Audergon, F. Dicenta
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Genetic relationships between peach (Prunus persica L.) and wild closely related species using SSR markers
auteur
Marie Foulongne, J. Minier, Thierry Pascal, J. Kervella
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Evaluation of INRA Prunus rootstocks for two japanese plum cultivars, Friar and Blackamber
auteur
Henri Duval, Jean-Luc Poessel
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Development of apricot microsatellites for cultivar identification
auteur
Jamila Chaïb, L.S. Hagen, Lamia Krichen, P. Lambert, Bruno Fady, J.M. Audergon
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Bioanalysis of apricot (Prunus armeniaca) fruit ESTs
auteur
Jérôme Grimplet, Charles Romieu, J.M. Audergon, Guy Albagnac, P. Lambert, J.P. Bouchet, S. Bureau, Marie Grotte, Barbara Gouble, Isabelle Marty, M. Reich, Nancy Terrier
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Inheritance of floral abortion in apricot tree
auteur
Jean-Michel J.-M. Legave, J.M. Audergon, J.C. Richard, Guy Clauzel, R. Viti
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Use of Myrobalan species (Prunus cerasifera) for almond and peach rootstocks
auteur
Henri Duval
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Inheritance of floral necrosis trait in apricot tree
auteur
Jean-Michel J.-M. Legave, J.M. Audergon, J.C. Richard, R. Viti, Guy Clauzel
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Studying inheritance of morphological and architectural traits on fruit trees: constraints and perspectives of INRA research programs
auteur
Evelyne E. Costes, P.E. Lauri, F. Laurens, J.M. Audergon, Jean-Michel J.-M. Legave, Nathalie Moutier, Andre Belouin, M. Lelezec, J. Claverie, F. Felort, J.L. Regnard
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Analysis of morphological and architectural traits of apricot cultivars grown in different environmental conditions
auteur
Dominique Fournier, Evelyne E. Costes, J.C. Salles, Vincent Segura, Guy Clauzel, J.M. Audergon, Jean-Michel J.-M. Legave
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Inheritance of organoleptic quality traits of apricot
auteur
Veronique Signoret, S. Bureau, M. Reich, Barbara Gouble, Guy Clauzel, Guy Albagnac, J.M. Audergon
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Location of peach, almond, apricot, plum and cherry genes on a syntenic Prunus linkage map
auteur
Elisabeth Dirlewanger, Patrick Cosson, P. Lambert, M.T. Dettori, Ignazio Verde, Daniel Esmenjaud, J.M. Audergon, R. Quarta, Pere Arus, Frédéric Laigret
article
Eucarpia symposium, Sep 2003, Angers, France
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titre
Testing of genetic control hypothesis for plum pox virus resistance in apricot
auteur
M. Rubio, P. Martínez-Gómez, F. Dicenta, J.M. Audergon
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Re-examination of an introgression procedure of a polygenic resistance to powdery mildew from wild Prunus davidiana into peach in the light of molecular data and monte-carlo simulations
auteur
Marie Foulongne, Thierry Pascal, F. Pfeiffer, J. Kervella
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Transcriptomics and proteomics tools towards ripening markers assisted selection in apricot
auteur
Jérôme Grimplet, Charles Romieu, F.X. Sauvage, P. Lambert, J.M. Audergon, Nancy Terrier
article
11. Eucarpia Symposium, Sep 2003, Angers, France
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titre
Comparative mapping of Prunus armeniaca, Prunus cerasifera x Prunus armeniaca and Prunus reference map
auteur
P. Lambert, Mireille Faurobert, Jean-Luc Poessel, J.M. Audergon
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Development of apricot microsatellites for cultivar identification
auteur
Jamila Chaïb, L.S. Hagen, Lamia Krichen, Bruno Fady, J.M. Audergon
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
Analysis of morphological and architectural traits of apricot varieties grown in different environmental conditions
auteur
Dominique Fournier, Evelyne E. Costes, J.C. Salles, Vincent Segura, Guy Clauzel, Jean-Michel J.-M. Legave, J.M. Audergon
article
EUCARPIA Symposium, Sep 2003, Angers, France
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titre
The key role of the eukariotic initiation factor 4E (elF4E) in plant-potyvirus interactions
auteur
Sandrine Ruffel, M.H. Dussault, A. Duprat, Alain Palloix, Benoît Moury, Frederic Revers, Abdelhafid Bendahmane, Christophe Robaglia, Carole Caranta
article
11. International Congress on molecular plant-microbe interactions, Jul 2003, St Petersbourg, Russia
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titre
The cap-binding protein, eIF4E, controls susceptibility to Lettuce mosaic potyvirus in Arabidopsis thaliana and lettuce
auteur
Valerie Nicaise, Sylvie S. German-Retana, Raquel Sanjuan, Frederic Revers, Thomas Guiraud, Martine Peypelut, Marie-Pierre Dubrana, Marianne Mazier, Brigitte B. Maisonneuve, Thierry Candresse, Christophe Robaglia, Carole Caranta, Olivier Le Gall
article
11. International Congress on molecular plant-microbe interactions, Jul 2003, St-Petersburg, Russia
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titre
Whole-plant regulation of root N uptake in A. thaliana: the search of the integrative mechanisms
auteur
Laurence Lejay, Stephane Munos, M. Cerezo, X. Gansel, M. Lepetit, Alain Gojon
article
5. Colloque Général de la Société Française de Physiologie Végétale, Jul 2003, Orsay, France
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titre
Efficiency and limits of marker-assisted selection for organoleptic quality in tomato
auteur
Mathilde M. Causse
article
7. International Congress, Jun 2003, Barcelone, Spain
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titre
Genetic and genomic analysis of organoleptic quality components in tomato fruit
auteur
Mathilde M. Causse
article
7. International Congress, Jun 2003, Barcelone, Spain
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titre
The eukaryotic translation initiation factor 4E (elF4E): a target for plant resistance against potyviruses
auteur
Sandrine Ruffel, M.H. Dussault, A. Duprat, Alain Palloix, Benoît Moury, Frederic Revers, Abdelhafid Bendahmane, Christophe Robaglia, Carole Caranta
article
7. International Congress, Jun 2003, Barcelone, Spain
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titre
Rôle du facteur d'initiation de la traduction eIF4E dans la sensibilité de la laitue au LMV
auteur
Valerie Nicaise, Sylvie German Retana, Rafael Sanjuán, Marie-Pierre Dubrana, Marianne Mazier, Brigitte Maisonneuve, Thierry Candresse, Carole Caranta, Olivier Le Gall
article
Journées des microbiologistes de l'INRA, May 2003, Dourdan, France
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titre
Molecular characterization of mo1, a recessive gene associated with Lettuce mosaic potyvirus resistance in lettuce
auteur
Valerie Nicaise, Sylvie German Retana, R. Sanjuan, Marie-Pierre Dubrana, Marianne Mazier, Brigitte B. Maisonneuve, Thierry Candresse, Carole Caranta, Olivier Le Gall
article
EUCARPIA Meeting Leafy vegetables conference 2003, Mar 2003, Noordwijkerhout, Netherlands
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titre
Lactuca virosa, a source of disease resistance genes for lettuce breeding: results and difficulties for gene introgression
auteur
Brigitte Maisonneuve
article
EUCARPIA Meeting Leafy vegetables conference 2003, Mar 2003, Noordwijkerhout, Netherlands
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titre
Rôle du facteur d'initiation de la traduction EIF4E dans la sensibilité de la laitue au LMV
auteur
Valerie Nicaise, Sylvie German Retana, R. Sanjuan, Marie-Pierre Dubrana, Marianne Mazier, Brigitte B. Maisonneuve, Thierry Candresse, Carole Caranta, Olivier Le Gall
article
9. Rencontres, Feb 2003, Aussois, France
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titre
Rôle du facteur d'initiation de la traduction 4E (EIF4E) dans la résistance aux potyvirus chez les solanacées: résistance hôte et résistance non hôte
auteur
Sandrine Ruffel, M.H. Dussault, R. Sanjuan, Chrystel Deulvot, A. Moretti, M.L. Lesage, Alain Palloix, M.C. Daunay, Carole Caranta
article
9. Rencontres, Feb 2003, Aussois, France
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titre
Clonage positionnel du gène de résistance vat (virus aphid transmission) chez le melon
auteur
J. Pauquet, E. Burget, Lynda Hagen, Michel M. Caboche, Michel Pitrat, Abdelhafid Bendahmane, Catherine Dogimont
article
9. Rencontres, Feb 2003, Aussois, France
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titre
Le locus récessif PVR2 contrôlant la résistance au potato virus y chez le piment correspond au facteur eucaryotique d'initiation de la traduction EIF4E
auteur
Sandrine Ruffel, M.H. Dussault, Alain Palloix, Benoît Moury, Abdelhafid Bendahmane, Christophe Robaglia, Carole Caranta
article
9. Rencontres, Feb 2003, Aussois, France
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titre
Sélection de souches de potato virus Y (PVY) adaptées à une résistance polygénique quantitative chez le piment (capsicum annuum L.) et une recherche des déterminants viraux du contournement
auteur
Valérie Ayme, Benoît Moury, Carole Caranta, Alain Palloix, Mireille Jacquemond
article
9. Rencontres, Feb 2003, Aussois, France
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titre
Impact du réchauffement climatique sur la phénologie des arbres fruitiers dans la vallée du Rhône
auteur
Marjorie Domergue, Nadine Brisson, Jean-Michel J.-M. Legave, V. Matthieu, Bernard Baculat, Bernard Seguin
article
Séminaire STICS, Jan 2003, Arles, France
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titre
Résistances de la laitue à Bremia lactucae: efficacité et limites
auteur
Brigitte B. Maisonneuve
article
Rencontres phytosanitaires légumes et fraises, Jan 2003, Balandran, France
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Poster communications

titre
Agronomical studies of transgenic lettuce with a nitrate reductase gene from tobacco
auteur
Brigitte B. Maisonneuve, V. Dubois, Marianne Mazier, Yves Y. Chupeau
article
EUCARPIA Meeting Leafy vegetables conference 2003, Mar 2003, Noordwijkerhout, Netherlands. CGN, 2003, Eucarpia leafy vegetables 2003
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titre
Eukaryotic translation initiation factor 4E (eIF4E) controls lettuce susceptibility to Lettuce mosaic virus
auteur
Valerie Nicaise, Sylvie German Retana, Rafael Sanjuán, Marie-Pierre Dubrana, Marianne Mazier, Brigitte Maisonneuve, Thierry Candresse, Carole Caranta, Olivier Le Gall
article
International Congress of Plant Pathology, Feb 2003, Christchurch, New Zealand. 2003
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titre
Obtention et études de laitues transgéniques comportant différentes portions de génome viral
auteur
Fabrice Flamain, Emmanuel Botton, Arnaud Boisnard, J. Mörbel, Colette Tourneur, Benoît Moury, Verane Sarnette, Brigitte B. Maisonneuve, Thierry Wetzel, Thierry Candresse, Yves Y. Chupeau, Olivier Le Gall, Marianne Mazier
article
9. Rencontres, Feb 2003, Aussois, France. 2003, Virologie végétale
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Reports

titre
Déterminants moléculaires de l'interaction Solanacées-potyvirus : rôle de eIF4E et des autres membres du complexe d'initiation de la traduction dans la résistance
auteur
Nelly Fabre
article
[Stage] Faculté des Sciences de Luminy. Université de la Méditerranée (Aix Marseille 2), Marseille, FRA. 2003, 32 p
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titre
Résistance induite du pêcher, Prunus persica (L.) Batsch cv. Rubira au puceron vert, Myzus persicae Sulzer: étude de la systémie et de l'implication des composés phénoliques
auteur
Elodie Piednoir
article
[Stage] Université d'Orléans (UO), FRA. 2003, 40 p
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titre
Recherche par bulk segregant analysis de marqueurs moléculaires liés aux résistances polygéniques au mildiou (Pseudoperonospora cubensis) chez le melon (Cucumis melo)
auteur
C. Struillou
article
[Stage] France. Université de Rennes 1 (UR1), Rennes, FRA. 2003, 32 p
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titre
Intégration du gène eIF4E codant pour l'initiation à la traduction dans la tomate et caractérisation préliminaire des transformants
auteur
A. Imbert-Fillols
article
[Stage] IUP Agrosciences. Université d'Avignon et des Pays de Vaucluse (UAPV), FRA. 2003, 28 p
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2002

Conference papers

titre
Durability of zucchini yellow mosaic virus resistances in cucurbits
auteur
Hervé Lecoq, Cecile Desbiez, Catherine Wipf-Scheibel, Myriam Girard, Michel Pitrat
article
Cucurbitaceae 2002, Dec 2002, Naples FL, United States
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titre
Evaluation a posteriori de l'efficacité d'un shéma d'introgression d'une résistance polygénique à l'oïdium chez le pêcher
auteur
Marie Foulongne, Thierry Pascal, J. Kervella
article
7. Rencontre, Oct 2002, Avignon, France
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titre
Traitements bio-informatiques de trois banques d'EST de fruits d'abricot
auteur
J.P. Bouchet
article
7. Rencontre, Oct 2002, Avignon, France
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titre
Synteny among Prunus genomes
auteur
Elisabeth Dirlewanger, Patrick Cosson, Annick Moing, Claude Poizat, M.J. Aranzana, M.T. Dettori, Ignazio Verde, P. Lambert, Lynda Hagen, J.M. Audergon, M.L. Badenes, R. Quarta, Frédéric Laigret, Pere Arus
article
7. Rencontre du groupe de biologie moléculaire des ligneux, Oct 2002, Avignon, France
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titre
Isolement et caractérisation de microsatellites chez le cèdre et l'abricotier
auteur
Jamila Chaïb, Bruno Fady, L.S. Hagen, J.M. Audergon
article
7. Rencontre, Oct 2002, Avignon, France
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titre
Approche protéomique de la résistance du pêcher au puceron vert
auteur
Mireille Faurobert, M.H. Sauge, Thierry Pascal, J. Kervella
article
7. Rencontre, Oct 2002, Avignon, France
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titre
Construction de deux cartes génétiques de l'abricotier (Prunus armeniaca) basées sur l'utilisation de la synténie et des cartes génétiques développées chez les autres espèces fruitières du genre Prunus
auteur
P. Lambert, L.S. Hagen, J.M. Audergon
article
7. Rencontre du groupe de biologie moléculaire des ligneux, Oct 2002, Avignon, France
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titre
Utilisation d'outils génomiques dans les programmes d'amélioration des plantes. Quelques exemples chez les plantes maraîchères
auteur
Michel Pitrat, Mathilde M. Causse
article
Colloque INRA, Oct 2002, Montpellier, France
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titre
Increase in phenolic content in the resistant peach cultivar 'Rubira' infested by the Green peach aphid, Myzus persicae
auteur
Jean-Luc Poessel, M.N. Corre, J. Kervella, J.P. Lacroze, M.H. Sauge
article
21. International Conference on Polyphenols, Sep 2002, Marrakech, Morocco
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titre
Recent advances in molecular breeding: the example of tomato breeding for flavor traits
auteur
L. Lecomte, A. Gautier, Aurélia Luciani, Philippe Duffe, Frederic F. Hospital, Michel Buret, Mathilde M. Causse
article
25. ISHS Symposium, Aug 2002, Toronto, Canada
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titre
Resistance to biotic stress in fruit trees
auteur
Yves Lespinasse, Charles Eric Durel, A. Eskes, Daniel Esmenjaud, Jean-Luc Poessel
article
26. IHC-Genetics and Breeding of Tree Fruits and Nuts, Aug 2002, Toronto, Canada
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titre
The ultra dense marker map of potato; new methods and new applications
auteur
H. van Os, J. van Vliet, Julie Bakker, Bernard Caromel, Véronique Lefebvre, F. Rousselle-Bourgeois, I. Barrena, E. Ritter, E. Isidore, D. Milbourne, Glenn Bryan, Robbie Waugh, H.J. van Eck
article
15. Triennal Conference of the EAPB, Jul 2002, Hambourg, Germany
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titre
Preliminary anchoring of the potato ultra high density map with the tomato map through a bridge progeny
auteur
S. Andrzejewski, Bernard Caromel, B. Ghareeb, Véronique Lefebvre, F. Rousselle-Bourgeois
article
15. Triennal Conference of the EAPB, Jul 2002, Hambourg, Germany
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titre
Application of a potato UHD genetic linkage map for BAC landing and contig initiation in a region of linkage group V
auteur
G.J. Bryan, H.J. van Eck, D. Milbourne, E. Isidore, K. Mclean, J. Mcnicoll, S. Linton, I. Tierney, A. Purvis, H. van Os, Julie Bakker, F. Rousselle-Bourgeois, E. Ritter, Robbie Waugh
article
15. Triennal Conference of the EAPB, Jul 2002, Hambourg, Germany
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titre
Breeding for a new generation of Prunus rootstocks: an example of marker-assisted selection
auteur
Elisabeth Dirlewanger, Marc M. Kleinhentz, Roger R. Voisin, Michel Claverie, Anne-Claire Lecouls, Daniel Esmenjaud, Jean-Luc Poessel, Mireille Faurobert, Pere Arus, Joachin Gomez-Aparisi, Cristos Xiloyannis, Mauro Di Vito
article
1. International Symposium, Jun 2002, Zaragoza, Spain
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titre
Apport des études de synténie: comparaison des génomes chez les rosacées fruitières
auteur
Elisabeth Dirlewanger, Annick Moing, C. Etienne, Patrick Cosson, Claude Poizat, Beatrice Denoyes-Rothan, E. Lerceteau-Köhler, Charles Eric Durel, L. Hibrand Saint-Oyan, Mireille Faurobert, J.M. Audergon, P. Lambert, Pere Arus, Frédéric Laigret
article
12. Colloque, May 2002, Bordeaux, France
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titre
Résistance aux nématodes à galles chez les prunus: un exemple de sélection assistée par marqueurs pour la création de nouveaux porte-greffes
auteur
Daniel Esmenjaud, Elisabeth Dirlewanger, Nathalie Bosselut, G. Salesses, A. Bonnet, R. Voisin, Véronique Bergougnoux, A.C. Lecouls, M. Claverie, Pere Arus, J. Gomez-Aparisi, C. Xiloyannis, M. Di Vito, Jean-Luc Poessel, Mireille Faurobert
article
12. Colloque, May 2002, Bordeaux, France
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titre
Cartographie moléculaire de facteurs de la qualité organoleptique des fruits en vue de leur utilisation en sélection
auteur
Mathilde M. Causse, L. Lecomte, Michel Buret, J. Kervella, Marie Foulongne, J.M. Audergon
article
12. Colloque sur les Recherches fruitières: les apports de la biologie moléculaire en arboriculture fruitère, May 2002, Bordeaux, France
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titre
Durable virus resistance in plants through conventional approaches. A challenge
auteur
Hervé Lecoq, Cecile Desbiez, Benoît Moury, Michel Pitrat
article
8. International Plant Virus Epidemiology Symposium, May 2002, Aschersleben, Germany
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titre
Taxonomic position of pepper veinal mottle potyvirus and pathotypic relationships with sources of resistance in peppers
auteur
Benoît Moury, Kahsay Gebre Selassie, Patrick Gognalons, Alain Palloix, Georges Marchoux
article
8. International Plant Virus Epidemiology Symposium, May 2002, Aschersleben, Germany
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titre
Inheritance of fruit quality traits in an apricot progeny. Influence of ethylene
auteur
J.M. Audergon, Barbara Gouble, S. Bureau, H. Chahine, Didier Mbéguié-A-Mbéguié, B. Fils-Lycaon, M. Reich, Alain Giard, Guy Albagnac
article
6. International Meeting on Biology and Biotechnology of the Plant Hormone Ethylene, Apr 2002, Murcie, Spain. 1 p
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titre
Regulation by ethylene of carotenoid biosynthesis in fruits of two contrasted apricot varieties. Poster
auteur
S. Bureau, Barbara Gouble, M. Reich, J.M. Audergon, Guy Albagnac
article
6. International Meeting on Biology and Biotechnology of the Plant Hormone Ethylene, Apr 2002, Murcia, Spain. 1 p
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titre
Genetic analysis of organoleptic quality components in fresh market tomato
auteur
Mathilde M. Causse, V. Saliba-Colombani, L. Lecomte, Michel Buret
article
Annual Main Meeting of the Society for Experimental Biology, Apr 2002, Swansea, United Kingdom
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titre
Cartographie fine d'un QTL majeur de résistance à Phytophthora capsici chez le piment en vue de sa caractérisation moléculaire et fonctionnelle
auteur
C. Boudet, Alain Palloix, Véronique Lefebvre
article
4. Rencontres de Phytopathologie/Mycologie, Mar 2002, Aussois, France
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titre
Analyse génétique de la résistance à l'oïdium chez le génotype de melon PI 124112
auteur
Marc Bardin, Laure Perchepied, Catherine Dogimont, Philippe C. Nicot, Michel Pitrat
article
4. Rencontres de Phytopathologie/Mycologie, Mar 2002, Aussois, France
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titre
La génétique au service de la protection des plantes : les programmes de l'INRA d'Avignon en amélioration de la résistance des arbres fruitiers
auteur
J. Kervella
article
Rencontre chercheurs - professionnels, Jan 2002, Avignon, France
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titre
Situacion practica de la lucha genetica contra los agentes patogenos de las hortalizas en cultivos protegidos, en la cuenca mediterranea
auteur
H. Laterrot
article
12. Symposium Internacioanl : Ecologia y produccion integrada en cultivos horticolas de invernadero, Jan 2002, Valencia, España
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Poster communications

titre
Résistance à Bremia lactucae chez la laitue. Poster
auteur
Brigitte B. Maisonneuve
article
4. Rencontres de Phytopathologie/Mycologie, Mar 2002, Aussois, France. 2002, Journées Jean Chevaugeon
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Reports

titre
Etude de l'intéraction du pêcher Prunus persica face au puceron vert Myzus persicae
auteur
F. Convers
article
[Stage] Institut Catholique des Sciences de la Vie et de la Terre (ICSVT), Le Puy en Velay, FRA. 2002, 48 p
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titre
Conception d'un outil de consultation de ressources génétiques sur le melon
auteur
B. Lepaul
article
[Stage] IUP. Université d'Avignon et des Pays de Vaucluse (UAPV), Montfavet, FRA. 2002, 57 p
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titre
Etude, chez la laitue, du déterminisme génétique de résistances à Bremia lactucae identifiées chez L. virosa
auteur
Wen Ying Rong
article
[Stage] Institut National Agronomique Paris Grignon (INAPG), Paris, FRA. 2002, 29 p
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titre
Etude de certains aspects de la transformation génétique de la tomate à des fins d'optimisation
auteur
Nicolas Veronese
article
[Stage] Institut Universitaire de Technologie d'Avignon (IUT d'Avignon), Avignon, FRA. 2002, 31 p
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titre
Marquage moléculaire du gène dominant Pot-2 impliqué dans la résistance au virus Y de la pomme de terre (PVY) chez la tomate
auteur
Peggy Cavallin
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 2002, 27 p
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titre
Première cartographie génétique de porte-greffes Prunus à l'aide de marqueurs AFLP
auteur
S. Passera
article
[Stage] Université d'Avignon et des Pays de Vaucluse (UAPV), FRA. 2002, 38 p
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2001

Conference papers

titre
Diversity of african vegetable Solanum species and its implications for a better understanding of plant domestication
auteur
R.N. Lester, M.C. Daunay
article
Symposium dedicated to the 100th birthday of Rudolf Mansfeld, Oct 2001, Gatersleben, Germany
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titre
Potyviridae: a major threat to cucurbit crops worldwide
auteur
Hervé Lecoq, Cecile Desbiez, Catherine Dogimont, Michel Pitrat
article
2. International Symposium on Cucurbits, Sep 2001, Tsukuba, Japan
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titre
Genetic control of fruit quality and maturation traits in melon
auteur
Christophe Périn, M.C. Gomez-Jimenez, Lynda Hagen, Catherine Dogimont, J.C. Pech, Alain Latche, Jean-Marc J.-M. Lelievre, Michel Pitrat
article
2. International Symposium, Sep 2001, Tsukuba, Japan
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titre
Apricot breeding: update and perspectives
auteur
B. Bassi, Jean Marc Audergon
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Characterization and influence of floral abortion in french apricot crop area
auteur
Jean-Michel J.-M. Legave, Jean-Claude Richard, Dominique D. Fournier
article
12. International Symaposium, Sep 2001, Avignon, France
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titre
Optimisation of apricot breeding by a joint conventional and molecular approach applied to the main agronomic traits. Abrigen project
auteur
Jean Marc Audergon, Alain Giard, Patrick Lambert, Alain Blanc, Frederic Gilles, Veronique Signoret, Jean-Claude Richard, Guy Albagnac, Sylvie Bureau, Barbara Gouble, Marie Grotte, Maryse Reich, Jean-Michel J.-M. Legave, Guy Clauzel, F. Dicenta, M. Scortichini, A.M. Simeone, R. Guerriero, R. Viti, P. Monteleone, S. Bartolini, J.M.S. Martins, J. Tsiantos, P. Psallidas
article
12. International Symposiuù, Sep 2001, Avignon, France
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titre
Experiments conducted by INRA on bacterial canker.
auteur
J.M. Audergon, A. Vigouroux
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Characterization of Orangé de Provence apricots (Prunus armeniaca L.) fruits at a commercial mature stage from a production area
auteur
Sylvie Bureau, H. Gouble, Maryse Reich, Guy Jacquemin, Guy Albagnac, K. Robini, L. Hashim, D.E. Ladeveze, Jean Marc Audergon, F. Gurrieri
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Architectural diversity of apricot trees: which morphological characters can be used to classify cultivars
auteur
Evelyne E. Costes, Dominique D. Fournier, Jean Marc Audergon, Jean-Michel J.-M. Legave, Guy Clauzel
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Inheritance of floral abortion in progenies of Stark Early Orangé apricot
auteur
Jean-Michel J.-M. Legave, Jean-Claude Richard, R. Viti
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Solédane", "Florilège" and Bergarouge" Avirine: three new apricot cultivars for french country
auteur
Jean Marc Audergon, Jean-Marie Duffillol, Frederic Gilles, Guy Clauzel, Dominique Chauffour, Alain Giard, Alain Blanc, J.M. Broquaire, B. Moulon
article
12. International Symposium on Apricot culture and decline, Sep 2001, Avignon, France
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titre
Prediction of sensory data by instrument measurements of representative apricot (Prunus armeniaca L.) cultivars
auteur
K. Robini, L. Hashim, D.E. Ladeveze, Sylvie Bureau, Barbara Gouble, Maryse Reich, Guy Jacquemin, Guy Albagnac, F. Gurrieri, Jean Marc Audergon
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Physiological and genetic studies on Apricot/Prunus rootstocks graft compatibility
auteur
Jean-Luc Poessel, Mireille Faurobert, Michele Loonis, Gilles Olivier, Marie Noëlle Corre, Veronique Restier, Jean Marc Audergon, Martin Masse
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Preliminary results on inheritance of floral anomalies trait through the study of stark early orange progenies
auteur
Jean-Michel J.-M. Legave, R. Viti, J.C. Richard
article
12. Congrès International, Sep 2001, Avignon, France
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titre
Characterization and inheritance of apricot leaf necrosis observed on Manicot cultivar after sharka inoculation
auteur
I. Guillet-Bellanger, P. Saussac, Jean Marc Audergon
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Genetic and physiological studies on apricot / Prunus graft compatibility
auteur
Jean-Luc Poessel, Mireille Faurobert, M.N. Corre, G. Olivier, Veronique Restier, F. Gurrieri, M. Massé, J.M. Audergon
article
12. Congrès International, Sep 2001, Avignon, France
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titre
Fruit ripening of contrasted apricot varieties: physical, physiological and biochemical changes
auteur
Sylvie Bureau, H. Chahine, H. Gouble, Maryse Reich, Guy Albagnac, Jean Marc Audergon
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Evaluation of a mapping strategy based on syntheny: using the Prunus reference map for apricot
auteur
Patrick Lambert, L.S. Hagen, Jean Marc Audergon, Pere Arus
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Seasonal protein variations in apricot bark
auteur
Mireille Faurobert, Laurent Gomez
article
12. International Symposium, Sep 2001, Avignon, France
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titre
National agreement for the evaluation of fruit tree cultivars and rootstocks
auteur
J. Lichou, Jean Marc Audergon, Caroline Pinet, C. Chamet, J.M. Broquaire
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Evaluation of resistance of apricot progeny (Vesta X Seo) to plum pox virus
auteur
B. Krska, I. Oukropec, Jean Marc Audergon, P. Kominek
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Modeling the apricot sugar contents in relation to fruit growth
auteur
Michel M. Génard, Francoise F. Lescourret, Jean Marc Audergon, Maryse Reich, Guy Albagnac
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Inhibition of apricot ethylene biosynthesis by CO2
auteur
Barbara Gouble, Maryse Reich, Patrick Varoquaux, Guy Albagnac, D. Mbeguie-A-Mbeguie, Jean Marc Audergon
article
12. International Symposium on Apricot Culture and Decline, Sep 2001, Avignon, France
resume
Due to a large amount of 'Bergeron' apricot (Prunus armeniaca L.) production (around 70 000 tons, nearly 50% of the French apricot production), this variety is frequently commercialized after cold storage at 1°C. Problem of chilling-injuries and/or quick evolution after cold storage are observed. Treatment with an ethylene inhibitor combined with a higher storage temperature could be a solution to maintain fruit quality up to the consumption. The objective of this study was to evaluate the practical and theoretical impact of CO2 treatment during a short storage period at 10°C.
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Breeding for compatible apricot rootstocks cumulating resistance to plum pox virus and root-knot nematodes: the P x Dasycarpa way
auteur
Jean-Luc Poessel, Mireille Faurobert, Daniel Esmenjaud, Elisabeth Dirlewanger, Marie-Claude Lemoine, F. Gurrieri, P. Michelot, S. Lafond
article
12. International Symposium, Sep 2001, Avignon, France
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Characterisation of apricot cultivars infected with plum pox virus using virus concentration in leaves
auteur
I. Guillet-Bellanger, Jean Marc Audergon
article
12. International Symposium, Sep 2001, Avignon, France
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Apricot storage with 1-MCP
auteur
H. Gouble, Patrick Varoquaux, J.M. Audergon, G. Regiroli
article
12. Congrès International, Sep 2001, Avignon, France
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titre
Prospection, characterization and assessment of apricot genetic resources in the mediterranean region for the production in arid and semi-arid areas
auteur
Jean Marc Audergon, M.J. Asins, R. Guerriero, R. Viti, P. Monteleone, S. Bartolini, M. Laghezali, Aurélia Mamouni, M. Mlika, R. Hellali, Lamia Krichen, M. Ben Mimoun, R. Gulcan Ege, S. Paydas, Guy Albagnac, Sylvie Bureau, H. Gouble, Marie Grotte, Maryse Reich, Jean-Michel J.-M. Legave, Guy Clauzel, Patrick Lambert, Alain Blanc, Jean-Claude Richard
article
12. International Symposium, Sep 2001, Avignon, France
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The regulation mechanisms of carotenoids biosynthesis in apricot (Prunus armeniaca)
auteur
G. Sarkissian, Isabelle Marty, Sylvie Bureau, Maryse Reich, Barbara Gouble, Marie Josephe Amiot, Guy Albagnac, Jean Marc Audergon
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Influence of grafting technique on macroscopical graft incompatibility symptoms: comparison of chip budding and ring budding
auteur
F. Gurrieri, G. Olivier, Mireille Faurobert, Jean-Luc Poessel
article
12. International Symposium, Sep 2001, Avignon, France
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titre
Prospection, characterization and assessment of apricot genetic ressources in the mediterranean region for the production in arid and semi-arid areas
auteur
Jean Marc Audergon, R. Guerriero, R. Viti, P. Monteleone, M.D. Laghezali, A. Mamouni, R. Hellali, L. Krichen, M. Ben Mimoun, S. Paydas, Jean-Michel J.-M. Legave, Patrick Lambert, Alain Blanc, Jean-Claude Richard, M.J. Asins, S. Bartolini, M.D. Mlika, R. Gulcan, G. Albagnac, Sylvie Bureau, Barbara Gouble, Marie Grotte, Marlis Reich, Guy Clauzel
article
International Symposium on Apricot Culture and Decline, Sep 2001, Avignon, France
resume
Under the frame of an EEC - INCO-DC programme, 'APRIMED' research project is under development with the objective to characterize, to preserve and to identify the apricot genetic resources in part of the Mediterranean Region (Turkey, Spain, Italy, France,. Morocco and Tunisia). An integrated and multidisciplinary approach divided in 3 main tasks has been developed. Prospection and preservation of genetic resources - During the prospection, 103 accessions have been identified in Morocco and S8 in Tunisia; while the preservation of 430 clones has been performed in France, 279 in Italy and 94 in Turkey.
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Influence of variety on the organoleptic quality of tomato
auteur
L. Hashim, K. Robini, D. Ladeveze, Mathilde M. Causse
article
Analyse sensorielle, Jul 2001, Dijon, France
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titre
Comparison of peach genotypes using an ecophysiological model. Application to fruit dry mass growth
auteur
Bénédicte Quilot-Turion, Michel M. Génard, Francoise F. Lescourret, J. Kervella
article
6. International Symposium, Jul 2001, Davis, CA, United States
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QTLs and genes controlling peach fruit quality
auteur
C. Etienne, Elisabeth Dirlewanger, Patrick Cosson, Laurence Svanella-Dumas, R. Monet, Annick Moing, Christophe Rothan, Catherine Bodénès, Christophe Plomion, J. Kervella
article
5. International peach symposium, Jul 2001, Davis, United States
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titre
Biochemical comparison of mature fruits from Prunus persica, Prunus davidiana and their hybrids
auteur
Annick Moing, Laurence Svanella-Dumas, Jean-Luc Poessel, Michèle Loonis, J. Kervella
article
5. International peach symposium, Jul 2001, Davis, United States
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titre
Breeding for Prunus rootstocks cumulating resistance to root-knot nematodes and favorable agronomic traits under mediterranean environments: a european project
auteur
Elisabeth Dirlewanger, G. Salesses, A. Bonnet, Marc Kleinhentz, Pere Arus, Daniel Esmenjaud, Nathalie Bosselut, R. Voisin, J. Gomez-Aparasi, Véronique Bergougnoux, A.C. Lecouls, C. Xiloyannis, Jean-Luc Poessel, Mireille Faurobert, M. Di Vito
article
5. International peach symposium, Jul 2001, Davis, United States
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titre
Introgression of a polygenic resistance to powdery mildew from a wild species Prunus davidiana into peach (Prunus persica (L.) Batsch), a case study of marker assisted selection in fruit tree
auteur
Marie Foulongne, Thierry Pascal, F. Pfeiffer, J. Kervella
article
5. International Symposium, Jul 2001, Davis CA, United States
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titre
Prunus davidiana and derived progenies: a valuable material for fruit quality studies
auteur
J. Kervella, Marie Foulongne, R. Kraif, Thierry Pascal, F. Pfeiffer, Michel Pradier, Annick Moing, Cédric Renaud, C. Etienne, Michel M. Génard, Bénédicte Quilot-Turion, Francoise F. Lescourret, Josiane Hostalery, Christophe Rothan, M. Reich
article
5. International peach symposium, Jul 2001, Davis, CA, United States
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titre
Preliminary observations on the resistance to sharka in peach and related species
auteur
Thierry Pascal, F. Pfeiffer, J. Kervella
article
5. International Peach symposium, Jul 2001, Davis, CA, United States
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titre
Current status of the Allium collections in France (april 2001)
auteur
Veronique Chovelon, Florence Esnault
article
ECP/GR vegetatively propagated Allium meeting, May 2001, Gatersleben, Germany
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titre
Chi-3P1, a class III chitinase of Capsicum annuum L
auteur
- Ching-Ming Cheng, Alain Palloix, Véronique Lefebvre
article
3. International Symposium, May 2001, Ancona, Italy
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titre
Recherche de génotypes de Cucumis melo résistants à Pseudomonas syringae pv. aptata
auteur
C.M.H. Riffaud, David Lejon, Didier Besombes, Nathalie Giovinazzo, Michel Pitrat, Cindy E. Morris
article
5. Congrès de la Société Française de Phytopathologie, Mar 2001, Angers, France
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titre
Evolution et extension des virus chez les solanacées
auteur
Georges Marchoux, Kahsay Gebre Selassie, R. Légnani, Benoît Moury, R. Nono-Womdim, Carole Caranta
article
5. Congrès de la Société Française de Phytopathologie, Mar 2001, Angers, France
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titre
Emergence et/ou introductions de nouveaux virus sur tomate
auteur
Kahsay Gebre Selassie, Georges Marchoux, Giuseppe Parrella, Brigitte Delecolle, Patrick Gognalons, André Moretti, Anne-Cécile Cotillon, Anne Dalmon, Olivier Dufour
article
5. Congrès de la Société Française de Phytopathologie, Mar 2001, Angers, France
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titre
Cartographie fine d'un locus de résistance récessif aux potyvirus chez le piment (Capsicum annuum L.) en vue de son clonage positionnel
auteur
Sandrine Ruffel, Germain Gaudinat, Ghislaine Nemouchi, M. Havard, Alain Palloix, Carole Caranta
article
8. Rencontres, Mar 2001, Aussois, France
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titre
Utilisation de lignées d'introgression pour la cartographie génétique des gènes de résistance Pot-1 et Am chez la tomate
auteur
Carole Caranta, Giuseppe Parrella, André Moretti, Marie-Laure Lesage, Patrick Gognalons, Caroline Morel, Kahsay Gebre Selassie, Mireille Jacquemond
article
8. Rencontres, Mar 2001, Aussois, France
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titre
Recherche de sources de résistance au watermelon chlorotic stunt begomovirus (WMCSV) chez le melon (Cucumis melo L.) et hérédité de la résistance
auteur
M. Taha Yousif, A. Khey-Pour, Bruno Gronenborn, Michel Pitrat, Catherine Dogimont
article
8. Rencontres, Mar 2001, Aussois, France
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titre
Ressources génétiques et résistance aux virus
auteur
Michel Pitrat
article
8. Rencontres, Mar 2001, Aussois, France
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titre
Déterminant moléculaire du contournement de la résistance de Lycopersicon hirsutum par le PVY
auteur
Caroline Morel, Laurent Guilbaud, Benoît Moury, Carole Caranta, Mireille Jacquemond
article
8. Rencontres, Mar 2001, Aussois, France
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titre
Réaction nécrotique portée par la variété d'abricotier Manicot après inoculation avec le virus de la sharka. Caractérisation et conséquences
auteur
J.M. Audergon
article
8. Rencontres, Mar 2001, Aussois, France
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titre
Etude des protéines de réserves végétatives du pêcher par électrophorèse en SDS-PAGE
auteur
Laurent Gomez, Mireille Faurobert, Marie Odile Jordan
article
1. Rencontres, Jan 2001, Autrans, France
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titre
An integrated genetic linkage map of pepper (Capsicum annuum)
auteur
I. Paran, Véronique Lefebvre, J.R. van Der Voort, Laurane Landry, R. van Wijk, H. Verbakel, Carole Caranta, K. Livingstone, M. Jahn, Alain Palloix, J. Peleman
article
9. Conference, Jan 2001, San Diego, United States
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titre
Calculation of ultra-high density genetic linkage maps of heterozygous outbreeders : chromosome 1 of potato as a model study
auteur
E. Isidore, H. van Os, S. Andrzejewski, Julie Bakker, J. Buntjer, Bernard Caromel, H. van Eck, B. Ghareeb, Véronique Lefebvre, D. Milbourne, E. Ritter, F. Rousselle-Bourgeois, Robbie Waugh
article
9. Conference, Jan 2001, San Diego CA, United States
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titre
Identification of quantitative trait loci involved in partial restriction of cucumber mosaic virus (CMV) long-distance movement in pepper
auteur
Carole Caranta, A.M. Daubèze, Stéphanie Pflieger, Véronique Lefebvre, A. Thabuis, Anne Blattes, Ghislaine Nemouchi, Therese Phaly, Patrick Signoret, Alain Palloix
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
An artificial inoculation method to test the resistance of pepper to Leveillula taurica on detached organs
auteur
Marc Bardin, Anne-Marie Daubèze, Muna E. Suliman, Corinne Romiti, Véronique Lefebvre, Youssif F. Mohamed, Alain Palloix, Philippe C. Nicot
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Constructing multiresistant genotypes of sweet pepper for cultivation in the tropics
auteur
E.A. Ahmed, A.M. Daubèze, Denis Lafortune, T. Depestre, R. Nono Wondim, C. Duranton, T. Berke, N.B. Gaddagimath, Ghislaine Nemouchi, Therese Phaly, Alain Palloix
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Looking for common genetic factors conferring resistance to Phytophthora nicotianae and P. capsici in the pepper line CM 334
auteur
M.B. Allagui, A.M. Daubèze, Alain Palloix
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Management of eggplant and pepper genetic resources in Europe : networks are emerging
auteur
M.C. Daunay, Evelyne Jullian, F. Dauphin
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Comparative mapping of resistance quantitative trait loci to Phytophthora capsici in three pepper accessions
auteur
Véronique Lefebvre, A. Thabuis, Stéphanie Pflieger, A.M. Daubèze, Carole Caranta, Therese Phaly, Anne Blattes, Ghislaine Nemouchi, Patrick Signoret, Alain Palloix
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Marker-assisted introgression of quantitative trait loci controlling resistance to Phytophthora capsici Leonian originating from different accessions
auteur
A. Thabuis, Véronique Lefebvre, A.M. Daubèze, Patrick Signoret, Therese Phaly, Anne Blattes, Ghislaine Nemouchi, M. Havard, Véronique de Conto, Alain Palloix
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Progress in the variation studies and genetic control of important viruses of peppers in mediterranean countries
auteur
Georges Marchoux, Kahsay Gebre Selassie, Benoît Moury, Patrick Gognalons, Carole Caranta, Alain Palloix
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Variability of pepper veinal mottle potyvirus isolates and their impact on breeding for resistance in peppers
auteur
Kahsay Gebre Selassie, Carole Caranta, Patrick Gognalons, Ghislaine Nemouchi, Benoît Moury, Alain Palloix, Georges Marchoux
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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titre
Evaluation of eggplant genetic resources for resistance to tomato spotted wilt virus (TSWV)
auteur
F. Pedron, Marie-Christine Daunay, Kahsay Gebre Selassie, Patrick Gognalons, Evelyne Jullian, Georges Marchoux
article
11. EUCARPIA Meeting, 2001, Antalya, Turkey
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Poster communications

titre
Résistance naturelle aux virus chez les plantes
auteur
Carole Caranta
article
3. Journées francophones de virologie, Apr 2001, Paris, France. 1 p., 2001
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titre
Mise au point d'un test de résistance au virus de la mosaïque de la laitue (LMV) sur plantules in vitro
auteur
Marianne Mazier, Fabrice Flamain, Aurélie Rieu, Sylvie Souche, Hervé Lot, E. Martin, Brigitte B. Maisonneuve
article
8. Rencontres, Mar 2001, Aussois, France. 2001, Virologie végétale
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titre
Difficultés dans l'utilisation des résistances identifiées chez des espèces sauvages : exemple de la résistance au virus de la mosaïque de la laitue (LMV) de Lactuca virosa
auteur
Brigitte B. Maisonneuve, Yannick Bellec, E. Martin, Hervé Lot
article
8. Rencontres, Mar 2001, Aussois, France. 2001, Virologie végétale
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Reports

titre
Construction d'un simulateur pour la maîtrise de la qualité des pêches par les opérations culturales en verger de pêchers. Rapport final 2001
auteur
Francoise F. Lescourret, N. Moitrier, Michel M. Génard, Bénédicte Quilot-Turion, Daniel Plenet, Robert Habib, Jacques Besset, Claude Bussi, T. Girard, A. Duc, Stephane Bellon, Christine de Sainte Marie, J.M. Crestin, J. Kervella, Gerard Pelloux, . Inra, . Conseil Régional Provence Alpes Cote d'Azur
article
INRA--B02651, 2001
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titre
Recherche de QTL impliqués dans la qualité des fruits de tomate en explorant les potentialités offertes par une espèce sauvage, L. hirsutum, apparentée à la tomate cultivée
auteur
L. Barrot
article
[Stage] France. Université de Rennes 1 (UR1), Rennes, FRA. 2001, 50 p
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titre
Cartographie fine et caractérisation phénotypique d'un QTL majeur de résistance aux potyvirus chez le piment (Capsicum annuum L.)
auteur
E. Guichard
article
[Stage] France. Université de Rennes 1 (UR1), Rennes, FRA. 2001, 35 p
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titre
Recherche de marqueurs pour la résistance au mildiou chez la pomme de terre
auteur
V. Fontaine
article
[Stage] IUT Sciences. Université d'Avignon et des Pays de Vaucluse (UAPV), FRA. 2001, 43 p
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titre
Validation et cartographie fine de QTL impliqués dans la qualité organoleptique des fruits de tomate
auteur
A. Gautier
article
[Stage] Université Pierre et Marie Curie - Paris 6 (UPMC), FRA. 2001, 50 p
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2000

Conference papers

titre
The candidate gene approach for the identification of genes and QTLs: on the frontier between genetics and physiology
auteur
Mathilde M. Causse
article
4. General meeting of the SFPV, Dec 2000, Besançon, France
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titre
Diversity and interactions between potyvirus resistance factors in pepper : keys for durable resistance
auteur
Carole Caranta, Benoît Moury, Kahsay Gebre Selassie, Sandrine Ruffel, Giuseppe Parrella, A.M. Daubèze, Alain Palloix
article
Durable resistance : key to sustainable agriculture, Nov 2000, Wageningen, Netherlands
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titre
Breeding pepper for durable resistance against worldwide potyvirus : the LIRA intertropical network program
auteur
Alain Palloix, E.A. Ahmed, A.M. Daubèze, Denis Lafortune, T. Depestre, R. Nono Wondim, C. Duranton, T. Berke
article
Durable resistance : key to sustainable agriculture, Nov 2000, Wageningen, Netherlands
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titre
Les incompatibilités de greffe chez les arbres fruitiers
auteur
Jean-Luc Poessel, Mireille Faurobert, F. Ermel-Fontaine
article
3. Rencontres du Groupe de la Sainte-Catherine, Nov 2000, Orléans, France
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titre
Salinity effect on pepper cultivars: interactive effect of sodium and zinc
auteur
Pierre Cornillon, Alain Palloix, Magali Auge
article
2.Balkan Symposium, Oct 2000, Thessaloniki, Greece
resume
The pepper (Capsicum annuum L.) cultivation takes place primarily in warm countries where the salinity problems are often responsible for the reduction of yield at the quantity and quality level. So the adaptation of pepper to salinity conditions is very important to the improvement of the production, which implies the knowledge about the pepper behaviour when sodium chloride is present in the substrate. The study allowed the comparison of 10 inbred lines of pepper in saline conditions and the study was managed in hydroponic culture comparing the behaviour of inbreed lines with 100 mM NaCl and without NaCl in nutrient solution. When 100 mM of sodium chloride was put into the nutrient solution, the reduction of growth varied between 47 % for HDA 1109 and more than 65 % for HDA 103 compared to the reference. These different behaviours seemed to be connected to a change in sodium and zinc balance in the blade. The best growth of HDA 1109 was obtained when the plant accumulated sodium in the blade (260 mM) with a low level of zinc (24 mg/kg DM). The lowest growth of HDA 103 was linked to a low concentration of sodium (59 mM) and an accumulation of zinc in the blade (153 mg/kg DM). Inbred lines Mi 71 and Mi 96 had an intermediary behavior with a weak growth and a marked accumulation of sodium and zinc in the blade
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titre
Les nouvelles races de Bremia et les résistances aux pucerons chez la laitue
auteur
Brigitte B. Maisonneuve
article
Journée Technique, Oct 2000, Chateaurenard, France
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titre
Epidémiologie et contrôle de l'oïdium du piment
auteur
Muna E. Suliman, Marc Bardin, Anne-Marie Daubèze, Youssif F. Mohamed, Philippe C. Nicot
article
Réunion, Oct 2000, Bordeaux, France
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titre
Transfer by marker-assisted backcross of QTLs confering resistance to Phytophthora capsici Leon originating from different accessions
auteur
A. Thabuis, Véronique Lefebvre, A.M. Daubèze, Anne Blattes, Therese Phaly, Patrick Signoret, Ghislaine Nemouchi, Alain Palloix
article
11. Meeting, Aug 2000, Paris, France
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titre
Transfer of QTLs controlling fruit quality traits into tomato elite lines by marker-assisted backcross
auteur
L. Lecomte, Philippe Duffe, N. Baffert, Frederic F. Hospital, Mathilde M. Causse
article
11. Meeting, Aug 2000, Paris, France
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titre
Disease resistance in tomato : practical situation
auteur
H. Laterrot
article
Eucarpia tomato 2000, Aug 2000, Varsovie, Poland. ⟨10.1007/s11738-000-0048-8⟩
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titre
Biological and molecular characterization of two tomato strains of potato virus Y (PVY)
auteur
Caroline Morel, Patrick Gognalons, Laurent Guilbaud, Carole Caranta, Kahsay Gebre Selassie, Georges Marchoux, Mireille Jacquemond
article
Eucarpia Tomato'2000, Aug 2000, Varsovie, Poland. ⟨10.1007/s11738-000-0050-1⟩
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titre
Molecular tagging of the Am gene from Lycopersicon hirsutum f. glabratum PI 134417 using AFLP markers
auteur
Giuseppe Parrella, Isabelle Hochu, Kahsay Gebre Selassie, Patrick Gognalons, André Moretti, Georges Marchoux, Carole Caranta
article
EUCARPIA Tomato '2000, Aug 2000, Varsovie, Poland. ⟨10.1007/s11738-000-0036-z⟩
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titre
Genetic resources of eggplant (Solanum melongena) and allied species : a new challenge for molecular geneticists and eggplant breeders
auteur
M.C. Daunay, R.N. Lester, Christiane Gebhardt, J.W. Hennart, M. Jahn, A. Frary, S. Doganlar
article
5. International Solanaceae Conference, Jul 2000, Nijmegen, Netherlands
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titre
Cartographie de locus de résistance au nématode à kyste chez la pomme de terre
auteur
Bernard Caromel, Didier Mugniery, Véronique Lefebvre, D. Ellisèche, F. Rouselle-Bourgeois
article
7. Journées du réseau AUF Biotechnologies végétales : amélioration des plantes et sécurité alimentaire, Jul 2000, Montpellier, France
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titre
Resistance of tomato to Botrytis cinerea
auteur
Philippe C. Nicot, A.L. Pellier, André Moretti, Carole Caranta, P. Roussel
article
12. International Botrytis Symposium, Jul 2000, Reims, France
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titre
Cartographie de QTL de résistance au nématode à kyste chez la pomme de terre
auteur
Bernard Caromel, Didier Mugniery, Daniel Ellissèche, Véronique Lefebvre, F. Rousselle-Bourgeois
article
7. Journées scientifiques, Jul 2000, Montpellier, France
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titre
Some comments on intraspecific classification of cultivars of melon
auteur
Michel Pitrat, P. Hanelt, K. Hammer
article
7. EUCARPIA Meeting on Cucurbit genetics and breeding, Mar 2000, Ma'ale Ha Hamisha, Israel
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titre
Construction of a reference genetic map of melon
auteur
Christophe Périn, Lynda Hagen, Catherine Dogimont, Véronique de Conto, L. Lecomte, Michel Pitrat
article
7. EUCARPIA Meeting on Cucurbit genetics and breeding, Mar 2000, Ma'ale Ha Hamisha, Israel
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titre
Genetic relationships between apricot (Prunus armeniaca L.) and related species using AFLP markers
auteur
L.S. Hagen, P. Lambert, J.M. Audergon, Bouchaïb Khadari
article
International symposium, Mar 2000, Montpellier, France
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titre
Marker-assisted selection for the transfer of QTLs controlling fruit quality traits into tomato elite lines
auteur
Mathilde M. Causse, L. Lecomte, N. Baffert, Philippe Duffe, Frederic F. Hospital
article
International symposium, Mar 2000, Montpellier, France
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titre
Introgression of a partial resistance to Phytophthora capsici Leon. into a pepper elite line by marker assisted backcrosses
auteur
A. Thabuis, Véronique Lefebvre, A.M. Daubèze, Patrick Signoret, Therese Phaly, Anne Blattes, Alain Palloix
article
International symposium, Mar 2000, Montpellier, France
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titre
Introgression of a partial resistance to Phytophthora capsici Leon. into a pepper elite line by marker assisted backcrosses
auteur
A. Thabuis, Véronique Lefebvre, A.M. Daubèze, Patrick Signoret, Therese Phaly, Ghislaine Nemouchi, Anne Blattes, Alain Palloix
article
International Symposium, Mar 2000, Montpellier, France
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titre
Metabolic and genetic study of fruit acidity in peach
auteur
Annick Moing, C. Etienne, Elisabeth Dirlewanger, Dominique Rolin, J. Kervella, Laurence Svanella-Dumas, J.P. Gaudillère, P. Raymond, R. Monet, Christophe Rothan
article
Nouveaux horizons en sciences et biotechnologies végétales, Mar 2000, Toulouse-Labège, France
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titre
Mapping QTLs and genes involved in tomato fruit weight and sugar content
auteur
Mathilde M. Causse, Michel Buret, V. Saliba-Colombani, C. Chevalier, Christophe Rothan
article
International conference on the status of plant and animal genome research, Jan 2000, San Diego CA, United States
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titre
Genetic resources of eggplant (Solanum melongena L.) and allied species : a new challenge for molecular geneticists and eggplant breeders
auteur
M.C. Daunay, Christiane Gebhardt, J.W. Hennart, M. Jahn, R. Neville Lester
article
International Conference on the Status of Plant and Animal Genome Research, Jan 2000, San Diego, United States
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titre
Development and utility of cleaved amplified polymorphic sequence (CAPS) and restriction fragment length polymorphisms (RFLP) markers linked to the Fusarium wilt resistance gene fom 2 in melon (Cucumis melo L.)
auteur
- Xiangyang Zheng, D.W. Wolff, S. Baudracco-Arnas, Michel Pitrat
article
International Conference on the Status of Plant and Animal Genome Research, Jan 2000, San Diego, United States
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titre
QTL and candidate gene approaches to analyse genes involved in peach fruit quality
auteur
C. Etienne, Annick Moing, Laurence Svanella, Elisabeth Dirlewanger, J. Kervella, Christophe Rothan
article
International Conference on the status of plant and animal genome research, Jan 2000, San Diego, United States
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titre
A genetic linkage map of eggplant
auteur
A. Frary, S. Doganlar, M.C. Daunay, R.N. Lester, S.D. Tanksley
article
International Conference on the Status of Plant and Animal Genome Research, Jan 2000, San Diego, United States
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Reports

titre
Influence des déterminants variétaux sur la qualité organoleptique de la tomate
auteur
N. Marquer
article
[Stage] Technopôle Brest-Iroise. Ecole Supérieure de Microbiologie et de Sécurité Alimentaire (ESMISAB), Plouzané, FRA. 2000, 72 p
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1999

Conference papers

titre
La sélection variétale de l'ail, perspectives d'évolution
auteur
Veronique Chovelon
article
Carrefour technique CEFEL, Dec 1999, Beaumont de Lomagne, France
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titre
L'approche gène candidat pour la caractérisation moléculaire et fonctionnelle des QTL de résistance à Phytophthora capsici et à d'autres parasites chez le piment
auteur
Véronique Lefebvre, Stéphanie Pflieger, Céline Labourey, Carole Caranta, A.M. Daubèze, Alain Palloix
article
Réunion, Nov 1999, Antibes, France
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titre
Analysis of stylar ribonucleases (S-Rnases) in an almond progeny of 'Ferralise x Tuono
auteur
Henri Duval, A. Boutard, Mireille Faurobert
article
11. GREMPA Seminar on pistachios and almonds, Sep 1999, Sanliurfa, Turkey
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titre
Contrôle génétique de la variation des teneurs en composés volatils chez la tomate
auteur
Mathilde M. Causse, V. Saliba-Colombani, Michel Buret, D. Langlois, Isabelle Lesschaeve
article
18. Journées Internationales Huiles et Extraits, Sep 1999, Digne les Bains, France. pp.323-328
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titre
Introgression of a polygenic resistance to Sphaerotheca pannosa in peach
auteur
Marie Foulongne, Thierry Pascal, F. Pfeiffer, J. Kervella
article
1. International powdery mildew conference, Aug 1999, Avignon, France
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titre
Effect of temperature on germination and germ tube elongation of Leveillula taurica.
auteur
Muna E. Suliman, Marc Bardin, Anne-Marie Daubèze, Youssif F. Mohamed, Philippe C. Nicot
article
1. Conference, Aug 1999, Avignon, France
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titre
Evaluation of peach and wild related species genotypes for resistance to Sphaerotheca pannosa.
auteur
M. Arregui, Thierry Pascal, F. Pfeiffer, Marie Foulongne, J. Kervella
article
1. International powdery mildew conference, Aug 1999, Avignon, France
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titre
Aggressiveness of Leveillula taurica on peppers.
auteur
Muna E. Suliman, Marc Bardin, A.M. Daubèze, Youssif F. Mohamed, Philippe C. Nicot
article
1. Conference, Aug 1999, Avignon, France
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titre
Virulence of Sphaerotheca fuliginea and Erysiphe cichoracearum on melon and genetic analysis of resistance of melon genotypes 'PI 124112' and 'PI 414723
auteur
Marc Bardin, Catherine Dogimont, Michel Pitrat, Philippe C. Nicot
article
1. Conference, Aug 1999, Avignon, France
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titre
Tomato farnesyl diphosphate synthase encoding genes are highly expressed during early fruit development and in young organs
auteur
J. Gaffé, A. Vidal, Mathilde M. Causse, Linda Stammitti, J.P. Carde, Philippe Gallusci
article
Tomato molecular biology, Jul 1999, York, CA, United States
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titre
Mapping QTLs and genes involved in tomato fruit weight and sugar content
auteur
Mathilde M. Causse, Michel Buret, V. Saliba-Colombani, C. Chevalier, Christophe Rothan
article
Tomato molecular biology, Jul 1999, York, CA, United States
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titre
Expression of phosphoenolpyruvate carboxylase during tomato fruit development
auteur
C. Guillet, Christophe Rothan, D. Just, N. Benard, Agnes Destrac, Mathilde M. Causse, P. Raymond
article
Tomato molecular biology, Jul 1999, York, CA, United States
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titre
New resistance against downy mildew and lettuce mosaic potyvirus in wild Lactuca spp
auteur
Brigitte B. Maisonneuve, Yannick Bellec, Sylvie Souche, Hervé Lot
article
Eucarpia leafy vegetables 99, Jun 1999, Olomouc, Czech Republic
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titre
Molecular linkage mapping of a diploid progeny between Solanum tuberosum and S. spegazzinii
auteur
Bernard Caromel, F. Rousselle Bourgeois, Véronique Lefebvre, Didier Mugniery
article
14. Triennial Conference of the European Association for Potato Research, May 1999, Sorrente, Italy
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titre
Genetic mapping of disease resistance genes in melon (Cucumis melo L.)
auteur
Catherine Dogimont, Christophe Périn, Lynda Hagen, Marc Bardin, Michel Pitrat
article
Scientific Cooperation in Agriculture, Apr 1999, Toulouse, France
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titre
Genetic analysis of resistance to bacterial wilt and late blight in tomato
auteur
N.H. Grimsley, H. Laterrot, Brigitte B. Mangin, P. Moreau, J. Olivier, Philippe Prior, P. Thoquet, J.F. Wang
article
Symposium, Apr 1999, Toulouse, France
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titre
Resistance to aphid-borne viruses in vegetable crops
auteur
Hervé Lecoq, Michel Pitrat
article
7. International Symposium, Apr 1999, Aguadulce (Almeria), Spain
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titre
Sélection assistée par marqueurs : transfert de QTL contrôlant la qualité organoleptique du fruit de tomate chez des lignées élites
auteur
Mathilde M. Causse, V. Saliba-Colombani, Philippe Duffe, Michel Buret
article
Marqueurs moléculaires et stratégies génomiques chez les végétaux, Mar 1999, Méribel, France
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titre
Différentes résistances au cucurbit aphid borne yellows luteovirus chez le melon contrôlées par deux gènes récessifs complémentaires
auteur
Catherine Dogimont, A. Thabuis, Michel Pitrat, Hervé Lecoq
article
7. Rencontres, Mar 1999, Aussois, France
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titre
Obtention de clones cDNA infectieux de PVY
auteur
Caroline Morel, Carole Caranta, Laurent Guilbaud, Mireille Jacquemond
article
7. Rencontres, Mar 1999, Aussois, France
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titre
Comparative mapping of virus resistance genes regions in solanaceous species
auteur
Carole Caranta, Stéphanie Pflieger, Véronique Lefebvre, Alain Palloix, Anne Blattes
article
International Conference on the Status of Plant and Animal Genome Research, Jan 1999, San Diego, United States
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titre
Genome distribution of potyvirus resistance genes in pepper
auteur
Carole Caranta, Alain Palloix, A.M. Daubèze, A. Thabuis, Véronique Lefebvre, Ghislaine Nemouchi, Therese Phaly, Anne Blattes
article
International Conference on the Status of Plant and Animal Genome Research, Jan 1999, San Diego, United States
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titre
The european prunus mapping project : results on peach (P. persica)
auteur
Elisabeth Dirlewanger, M.T. Dettori, Ignazio Verde, M. Viruel, Annick Moing, Veronique Pronier, J. Kervella, R. Monet, R. Quarta
article
7. Conference, Jan 1999, San Diego, United States
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Poster communications

titre
Mapping of the virulence determinants in lettuce mosaic virus spontaneous variants
auteur
Olivier Le Gall, E. Redondo, Brigitte B. Maisonneuve, Sylvie Souche, Hervé Lot, Thierry Candresse
article
9. International congress, Jul 1999, Amsterdam, Netherlands. 2000, Molecular plant-microbe interactions. Book of abstracts
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titre
Transgenic lettuce for lettuce mosaic potyvirus resistance
auteur
Marianne Mazier, Sylvie Dinant, Yannick Bellec, Fabienne Gaudefroy, Yves Y. Chupeau, Brigitte B. Maisonneuve
article
Eucarpia Leafy vegetables, Jun 1999, Olomouc, Czech Republic. Palacky University, 1999, Eucarpia leafy vegetables 99
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Poster communications
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Reports

titre
Etude de l'expression transcriptionnelle et traductionnelle de gènes en réponse à l'infection par Phytophthora capsici chez le piment
auteur
Céline Labourey
article
[Stage] Ecole Nationale d'Industrie Laitière (ENIL), Mamirolle, FRA. 1999, 28 p
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titre
Marquage moléculaire par PCR spécifique du génome de la pomme de terre. Etude de polymorphisme au sein d'une population
auteur
Catherine Massonnet
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), FRA. 1999, 49 p
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titre
Spécificité vis a vis de différentes souches, des QTL de résistance au virus de la mosaïque du concombre (CMV) chez le melon
auteur
L. Lecomte
article
[Stage] France. Université de Rennes 1 (UR1), Rennes, FRA. 1999, 41 p
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1998

Conference papers

titre
Construction of a genetic map of melon with molecular markers and horticultural traits
auteur
Christophe Périn, Lynda Hagen, Catherine Dogimont, Véronique de Conto, Michel Pitrat
article
Cucurbitaceae'98, Nov 1998, Pacific Grove, United States
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titre
Cucurbit viruses : the classics and the emerging
auteur
Hervé Lecoq, G.C. Wisler, Michel Pitrat
article
Cucurbitaceae'98, Nov 1998, Pacific Grove, United States
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titre
Resistance to fungal diseases of foliage in melon
auteur
Michel Pitrat, Catherine Dogimont, Marc Bardin
article
Cucurbitaceae'98, Nov 1998, Pacific Grove, United States
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titre
L'enroulement chlorotique de l'abricotier : symptomatologie, gamme d'hôtes et méthode de lutte
auteur
Jean-Marc Lemaire, Jean-Pierre Jullian, Halina Ferrière, J.M. Audergon, Henri Duval
article
Journées techniques nationales de l'agriculture biologique. Arboriculture 1998, Nov 1998, Cavaillon, France
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titre
Resistance to bacteria in tomato
auteur
H. Laterrot
article
15. Eucarpia 1998 general congress, Sep 1998, Viterbo, Italy
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titre
QTLs for organoleptic quality in fresh market tomato
auteur
V. Saliba, Mathilde M. Causse, J. Philouze, Michel Buret, D. Langlois, Sylvie Issanchou, Isabelle Lesschaeve
article
15. Eucarpia General Congress, Sep 1998, Viterbo, Italy
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titre
In vitro regeneration of tomato
auteur
Maëlle Carré, Denis Duplat, Patrick Rousselle
article
15. Eucarpia General Congress, Sep 1998, Viterbo, Italy
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titre
Efficiency of recurrent selection for improving adaptation and fruit quality in tomato
auteur
Patrick Rousselle, Mathilde M. Causse, E. Pepey, J. Philouze
article
15. Eucarpia General Congress, Sep 1998, Viterbo, Italy
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titre
Efficiency of resistance to alfalfa mosaic virus in Lycopersicon esculentum PI134417 under different environmental and biological conditions
auteur
Giuseppe Parrella, H. Laterrot, Kahsay Gebre Selassie, Georges Marchoux
article
6. Convegno annuale SIPAV. Patologia vegetale e agricoltura sostenibile, Sep 1998, Campobasso, Italy. pp.162
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titre
Spectrum of resistance to root knot nematodes and inheritance of heat stable resistance in pepper (Capsicum annuum L.)
auteur
C. Djian-Caporalino, Lucette Pijarowski, Ariane Januel, Véronique Lefebvre, Therese Phaly, Alain Palloix, A. Dalmasso, Pierre Abad
article
10. Meeting, Sep 1998, Avignon, France
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titre
Breeding multiresistant bell peppers for intertropical cultivation conditions : the Lira program
auteur
Alain Palloix, T. Depestre, A.M. Daubèze, Denis Lafortune, R. Nono Wondim, Ahmed Elamin Ali, C. Duranton
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
Candidate gene approach for identifying QTLs involved in pepper/pathogen interactions
auteur
Stéphanie Pflieger, Véronique Lefebvre, Anne Blattes, Carole Caranta, Alain Palloix
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
The use of wild genetic resources for eggplant (Solanum melongena) breeding. 1. Seed dormancy in four Solanum species
auteur
M.C. Daunay, M.M. Poveda-Aguilar, R.N. Lester, Evelyne Jullian
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
Genetic linkage between the y locus and the capsanthin-capsorubin synthase gene : a discriminating tool of red and yellow fruited peppers
auteur
Véronique Lefebvre, A. Badillo, A.M. Daubèze, Anne Blattes, C. Ferrière, B. Camara, Alain Palloix
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
The use of wild genetic resources for eggplant (Solanum melongena breeding. 2. Crossability and fertility of interspecific hybrids
auteur
M.C. Daunay, R.N. Lester, Anne Dalmon, M. Ferri, W. Kapilima, M.M. Poveda Aguilar, Evelyne Jullian
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
Potyvirus resistance in pepper : AFLP mapping and characterization of the resistance mechanisms controlled by the Pvr4 and pvr5 genes
auteur
Carole Caranta, A. Thabuis, A. Romero, A.M. Daubèze, Anne Blattes, Ghislaine Nemouchi, J.C. Chauvet, F. Ponz, Alain Palloix
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
Characterising and fine mapping of the Me3 gene conferring heat-stable resistance to root-knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.) using AFLPs
auteur
C. Djian-Caporalino, Lucette Pijarowski, Ariane Januel, Véronique Lefebvre, Carole Caranta, J.C. Chauvet, Anne Blattes, Alain Palloix, A. Dalmasso, Pierre Abad
article
10. Meeting, Sep 1998, Avignon, France
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titre
Production and characterization of somatic hybrids of eggplant (Solanum melongena L.) with its wild relatives
auteur
C. Collonier, I.S. Mariska, M.C. Daunay, A. Servaes, G. Ducreux, D. Sihachakr
article
10. Meeting EUCARPIA, Sep 1998, Avignon, France
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titre
Two-dimensional protein electrophoresis study of zygotic and somatic embryogenesis in cypress (Cupressus sempervirens L.)
auteur
A. Sallandrouze, Mireille Faurobert, Mohamed El Maataoui, H.E. Espagnac
article
3. Siena 2D Electrophoresis Meeting, Aug 1998, Sienne, Italy
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titre
Evaluation of the interspecific progeny Prunus persica cv Summergrand x Prunus davidiana for disease resistance and some agronomic features
auteur
Thierry Pascal, F.G. Pfeiffer, M.H. Sauge, Daniel Esmenjaud
article
4. International Peach Symposium, Jun 1998, Bordeaux, France
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titre
Variability of tomato spotted wilt virus isolates in relation with stability of resistance on pepper and tomato genotypes
auteur
Kahsay Gebre Selassie, Patrick Gognalons, Benoît Moury, Alain Palloix, Georges Marchoux
article
4. International Symposium on tospoviruses and thrips in floral and vegetable crops, May 1998, Wageningen, Netherlands
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titre
Variabilité génétique et prise en compte des caractéristiques de l'arbre dans les programmes d'amélioration génétique des espèces fruitières ligneuses à l'INRA
auteur
F. Laurens, Jean Marc Audergon, J. Claverie, Henri Duval, E. Germain, J. Kervella, M. Le Lezec, J.M. Lespinasse
article
11. Colloque sur les recherches fruitières, Mar 1998, Montpellier, France
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titre
QTLs for organoleptic quality in fresh market tomato
auteur
V. Saliba, Mathilde M. Causse, J. Philouze, Michel Buret, D. Langlois, Isabelle Lesschaeve
article
International Conference on the status of plant and animal genome research, Jan 1998, San Diego, United States
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titre
Disease resistance genes analogs as candidates for genes and QTLs involved in pepper/pathogens interactions
auteur
Stéphanie Pflieger, Véronique Lefebvre, Anne Blattes, Carole Caranta, A.M. Daubèze, Ghislaine Nemouchi, Therese Phaly, Alain Palloix
article
International Conference on the status of plant and animal genome research, Jan 1998, San Diego, United States
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Reports

titre
Saturation d'une carte intraspécifique de la tomate et cartographie de gènes et de QTL intervenant dans la croissance et la qualité du fruit
auteur
L. Gervais
article
[Stage] Ecole Nationale Supérieure Agronomique. Université de Rennes 1 (UR1), FRA. 1998, 43 p
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titre
Influence de quelques paramètres sur la régénération in vitro de la tomate (Lycopersicon esculentum Mill.)
auteur
Radia Ayadi
article
[Stage] France. Université de Rennes 1 (UR1), Rennes, FRA. 1998, 50 p
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titre
Etude de la qualité des abricots. Caractérisation du déterminisme génétique des principaux critères de qualité chez l'abricotier
auteur
C. Mathieu
article
[Stage] IUT. Université de Rouen, Evreux, FRA. 1998, 62 p
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titre
Etude des caractères phénologiques, agronomiques morphologiques et biochimiques d'une descendance de prunier japonais
auteur
Caroline Agostini
article
[Stage] Université Montpellier 2 (Sciences et Techniques) (UM2), Montpellier, FRA. 1998, 29 p
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1997

Conference papers

titre
Stratégies de lutte contre les virus des cultures maraîchères. Exemple des solanacées
auteur
Georges Marchoux, Kahsay Gebre Selassie, Alain Palloix, A.M. Daubèze
article
5. Conférence Internationale, Dec 1997, Tours, France
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titre
In vivo analysis of the regulation of starch synthesis in maize grain using a quantitative genetic approach. Poster
auteur
C. Thévenot, M. Sene, Agnès Leonardi, Mathilde M. Causse, L. Prioul, L. Prioul
article
3. Colloque général de la SFPV, Dec 1997, Toulouse, France
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titre
Use of molecular markers for improving tomato quality and disease resistances
auteur
Mathilde M. Causse
article
Colloque Franco-Israélien, Oct 1997, Versailles, France
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titre
QTLs for quality and flavour in fresh market tomatoes
auteur
Mathilde M. Causse, V. Saliba, J. Philouze, Michel Buret, D. Langlois, Isabelle Lesschaeve
article
Tomato Breeders Roundtable, Oct 1997, Ithaca, United States
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titre
Inheritance of stylar ribonucleases in two almond progenies and their correlation with self-compatibility
auteur
R. Boskovic, K.R. Tobutt, M. Rovira, Melanie Romero, I. Battle, Henri Duval
article
2. International symposium on pistachios and almonds, Aug 1997, Davis, United States
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titre
Breeding for multiresistance in peach tree
auteur
J. Kervella, Thierry Pascal, F. Pfeiffer, Elisabeth Dirlewanger
article
4. International Symposium, Jun 1997, Bordeaux, France
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titre
Taking into account relationships between ecophysiological processes in the breeding for peach tree characteristics
auteur
J. Kervella, F. Pfeiffer, Loic L. Pagès, Michel M. Génard, Valérie Serra
article
4. International peach symposium, Jun 1997, Bordeaux, France
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titre
Evaluation of the interspecific progeny Prunus persica cv Summergrand X Prunus davidiana for disease resistance and some agronomic features
auteur
Thierry Pascal, J. Kervella, F.G. Pfeiffer, M.H. Sauge, Daniel Esmenjaud
article
4. International Symposium, Jun 1997, Bordeaux, France
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titre
Mapping quantitative trait loci controlling peach leaf curl resistance
auteur
M.A. Viruel, Delphine Madur, Elisabeth Dirlewanger, Thierry Pascal, J. Kervella
article
4. International Peach Symposium, Jun 1997, Bordeaux, France
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titre
Peach breeding in France
auteur
Thierry Pascal, R. Monteux-Caillet
article
4. International Symposium, Jun 1997, Bordeaux, France
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titre
Pollination of three main apricot cultivars in France
auteur
J.M. Audergon, J.M. Dufillol, Caroline Pinet, P. Blanc
article
11. International Symposium, May 1997, Veria, Greece
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titre
Contribution to the study of inheritance of the character self-incompatibility in apricot
auteur
J.M. Audergon, R. Guerriero, P. Monteleone, R. Viti
article
11. International Symposium, May 1997, Veria, Greece
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titre
Susceptibility of apricot cultivars to plum pox virus
auteur
I. Karayiannis, B. Di Terlizzi, J.M. Audergon
article
11. International Symposium, May 1997, Veria, Greece
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titre
Effect of ethylene on certain quality parameters of apricot fruit (Prunus armeniaca L.) during maturation and postharvest evolution
auteur
H. Chahine, Barbara Gouble, J.M. Audergon, M. Souty, Guy Albagnac, Guy Jacquemin, M. Reich, M. Hugues
article
11. International Symposium, May 1997, Veria, Greece
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titre
Results of investigation of some resistant and susceptible to sharka apricot cultivars and hybrids
auteur
D. Ogasanovic, R. Plazinic, J.M. Audergon
article
11. International Symposium, May 1997, Veria, Greece
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titre
Apricot breeding in France : 2 new apricot selections for french growers
auteur
J.M. Audergon, Dominique Chauffour, Guy Clauzel, J.M. Duffillol, F. Gilles, J.M. Broquaire, Laura Estève
article
11. International Symposium, May 1997, Veria, Greece
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titre
Diversity, history and production of cultivated cucurbits
auteur
Michel Pitrat, M. Chauvet, C. Foury
article
1. International Symposium, May 1997, Adana, Turkey
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titre
Régulation de l'absorption du nitrate chez les ligneux fruitiers
auteur
Marie Odile M. O. Jordan, J.P. Goutouly
article
Séminaire, Apr 1997, Montpellier, France
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titre
Projet de clonage du gène Vat de résistance du melon au puceron Aphis gossypii. Communication orale
auteur
Lynda Hagen, Catherine Dogimont, Marianne Mazier, Lise Jouanin, Michel Pitrat
article
Réunion de l'AIP Gènes de résistance aux maladies, Mar 1997, Isle sur la Sorgue, France
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Resistance to cucurbit aphid-borne yellows luteovirus in Cucumis melo L. : screening and genetical analysis. Communication orale
auteur
Catherine Dogimont, A. Bussemakers, S. Slama, J. Martin, Hervé Lecoq, Michel Pitrat
article
Luteovirus, Mar 1997, Cirencester, United Kingdom
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titre
Transformation génétique de la pomme de terre pour la résistance au PVY (Potyvirus)
auteur
Patrick Rousselle, Laura Chauvin, Christophe Robaglia, Jean-Pierre Albouy, F. Rousselle-Bourgeois, Colette Tourneur
article
6. Rencontres de virologie végétale, Mar 1997, Aussois, France
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1996

Conference papers

titre
Confirmation of incompatibility alleles in almond cultivars prodicted by analysis of stylar ribonucleases
auteur
Henri Duval, I. Batlle, R. Boskovic, K.R. Tobutt
article
10. Colloque du GREMPA, Oct 1996, Meknès, Morocco
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titre
La progression d'Eurytoma amygdali en France et les méthodes de lutte possibles
auteur
Henri Duval, Pascal Froment
article
10. Colloque du GREMPA, Oct 1996, Meknès, Maroc
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titre
Recherche de références sur les teneurs foliaires en éléments minéraux chez l'amandier
auteur
D. Courtial, Pascal Froment, Henri Duval
article
10. Colloque du GREMPA, Oct 1996, Meknès, Maroc
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1995

Conference papers

titre
Décomposition phénotypique et génétique de résistances complexes à plusieurs virus chez le piment (Capsicum annum L.) : organisation des facteurs de résistance sur le génome
auteur
Carole Caranta, Alain Palloix, Véronique Lefebvre, A.M. Daubèze, Kahsay Gebre Selassie, Georges Marchoux
article
AIP Gènes de résistance, Nov 1995, Seillac, France
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titre
Genetic mapping of QTL responsible for resistance to bacterial wilt in tomato variety Hawaii 7996
auteur
Philippe Thoquet, Jocelyne Olivier, Béatrice Bazin, Philippe Moreau, Jean Luc Pariente, Henri Laterrot, Brigitte B. Mangin, Nigel Grimsley
article
AIP gènes de résistance aux maladies, Nov 1995, Seillac, France
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titre
Cartographie génétique des facteurs de résistance à Globodera pallida et au virus de l'enroulement chez la pomme de terre
auteur
Bernard Caromel, Françoise Rousselle-Bourgeois
article
AIP Gènes de résistance aux maladies, Nov 1995, Seillac, France
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1994

Conference papers

titre
Management of a collection of Solanum species for eggplant (Solanum melongena) breeding purposes
auteur
M.C. Daunay, Anne Dalmon, R.N. Lester
article
4. International Solanaceae Conference, 1994, Adelaide, Australia
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1993

Conference papers

titre
Caractérisation biochimique du métabolisme anaérobie de l'abricot, incidence variétale
auteur
Sylvaine Bitteur, François-Xavier Sauvage, Clotilde Verries, Jean Sarris, M Souty, Jean-Patrice Robin, Jean-Marc Audergon
article
2. Rencontres sur l'abricotier, 1993, Avignon, France
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1991

Reports

titre
Multiplication in vitro de Leveillula taurica sur feuilles de piment (Capsicum annuum): essai d'application à la sélection
auteur
Annick Chiltz
article
[Stage] Centre de Formation et de Promotion Horticole (CFPH), Ecully cedex, FRA. 1991, 33 p
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